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COVID-19 is caused by severe acute respiratory SARS-CoV-2. Regardless of the availability of treatment strategies for COVID-19, effective therapy will remain essential. A promising approach to tackle the SARS-CoV-2 could be small interfering (si) RNAs. Here we designed the small hairpin RNA (named as shRNA688) for targeting the prepared 813 bp Est of the S protein genes (Delta). The conserved and mutated regions of the S protein genes from the genomes of the SARS-CoV-2 variants in the public database were analyzed. A 813 bp fragment encoding the most part of the RBD and partial downstream RBD of the S protein was cloned into the upstream red florescent protein gene (RFP) as a fusing gene in the pCMV-S-Protein RBD-Est-RFP plasmid for expressing a potential target for RNAi. The double stranded of the DNA encoding for shRNA688 was constructed in the downstream human H1 promoter of the plasmid in which CMV promoter drives enhanced green fluorescent protein (EGFP) marker gene expression. These two kinds of the constructed plasmids were co-transfected into HEK293T via Lipofectamine 2000. The degradation of the transcripts of the SARS-CoV-2 S protein fusing gene expressed in the transfected HEK293T treated by RNAi was analyzed by RT-qPCR with a specific probe of the targeted SARS-CoV-2 S protein gene transcripts. Our results showed that shRNA688 targeting the conserved region of the S protein genes could effectively reduce the transcripts of the S protein genes. This study provides a cell model and technical support for the research and development of the broad-spectrum small nucleic acid RNAi drugs against SARS-CoV-2 or the RNAi drugs for the other hazard viruses which cause human diseases.

Scientific understanding of regional wind speed variation characteristics and driving mechanisms is of great practical significance to the management and rational utilization of wind energy resources, ecological protection and governance, and sustainable development of agriculture. Taking the Shiyang River Basin in inland river basin of monsoon marginal zone as the study area, this paper analyzed wind speed changes and influencing factors at different time and space scales. The results showed that, first of all, the annual average wind speed showed a downward trend as a whole in Shiyang River Basin, and the change of spring wind speed and the decreasing frequency of daily max-wind speed above Grade 5 were the main contributors. The turning point of annual average wind speed was consistent with that in spring and autumn (1981) and earlier than that in winter and summer (1982). Secondly, as far as the wind speed change tendency rate is concerned, before the turning point, Yongchang area had the largest decline rate with obvious “2000 m effect”, while after the turning point, Wuwei Basin had the largest rise rate with obvious “basin effect”. Focusing on the downstream desert oasis region, the sand-driving wind speed showed a decreasing trend as a whole, and showed a “cliff-like” decline after 1988. The rising trend of sand-driving wind speed after 2014 will pose greater challenges to ecological governance. Finally, under the background of unbalanced global warming, the wind speed changes in different periods were driven by the circulation system changes. In 1970–1981, the decline of the wind speed was the result of the combined action of Arctic Oscillation and Asia Polar Vortex. However, after 1981, it was affected by North Atlantic Oscillation, Asian Meridional Circulation, Tibet Plateau Circulation, India-Burma Trough, Western Pacific Subtropical High and Northern Hemisphere Subtropical High.

Background: After the 2009 influenza A(H1N1)pdm09 pandemic, China established its first severe acute respiratory infections (SARI) sentinel surveillance system. Methods: We analyzed data from SARI cases in 10 hospitals in 10 provinces in China from February 2011 to October 2013. Results: Among 5,644 SARI cases, 330 (6%) were influenza-positive. Among these, 62% were influenza A and 38% were influenza B. Compared with influenza-negative cases, influenza-positive SARI cases had a higher median age (20.0 years vs.11.0, p = 0.003) and were more likely to have at least one underlying chronic medical condition (age adjusted percent: 28% vs. 25%, p < 0.001). The types/subtypes of dominant strains identified by SARI surveillance was almost always among dominant strains identified by the influenza like illness (ILI) surveillance system and influenza activity in both systems peaked at the same time. Conclusions: Data from China's first SARI sentinel surveillance system suggest that types/subtypes of circulating influenza strains and epidemic trends among SARI cases were similar to those among ILI cases. [ABSTRACT FROM AUTHOR]

The increase in the frequency and intensity of extreme weather events around the world has led to the frequent occurrence of global disasters, which have had serious impacts on the society, economic and ecological environment, especially fragile arid areas. Based on the daily maximum temperature and daily minimum temperature data of four meteorological stations in Shiyang River Basin (SRB) from 1960 to 2015, the spatio-temporal variation characteristics of extreme temperature indices were analyzed by means of univariate linear regression analysis, Mann–Kendall test and correlation analysis. The results showed that the extreme temperatures warming indices and the minimum of daily maximum temperature (TXn) and the minimum of daily minimum temperature (TNn) of cold indices showed an increasing trend from 1960 to 2016, especially since the 1990s, where the growth rate was fast and the response to global warming was sensitive. Except TXn and TNn, other cold indices showed a decreasing trend, especially Diurnal temperature (DTR) range, which decreased rapidly, indicating that the increasing speed of daily min-temperature were greater than of daily max-temperature in SRB. In space, the change tendency rate of the warm index basically showed an obvious altitude gradient effect that decreased with the altitude, which was consistent with Frost day (FD0) and Cool nights (TN10p) in the cold index, while Ice days (ID0) and Cool days (TX10p) are opposite. The mutation of the cold indices occurred earlier than the warm indices, illustrating that the cold indices in SRB were more sensitive to global warming. The change in extreme temperatures that would have a significant impact on the vegetation and glacier permafrost in the basin was the result of the combined function of different atmospheric circulation systems, which included the Arctic polar vortex, Western Pacific subtropical high and Qinghai-tibet Plateau circulation.

In order to improve the effect of chemical production management and improve the information level of management, this paper attempts to study chemical production management by integrating the Internet of Things (IoT) technology into the design of the parts quality management system, with a view to achieving automatic acquisition and detection. The study finds that the data information tested by the wireless interface is basically correct within 15 metres around the factory, showing that the system designed in this paper can greatly improve the collection, acquisition and accessing of the quality management information of factories and has certain application value.