Search

Your search keyword '"Jullien, Frederic"' showing total 15 results
Start Over Author "Jullien, Frederic"
15 results on '"Jullien, Frederic"'

2. Virus-induced gene silencing of the two squalene synthase isoforms of apple tree ( Malus × domestica L.) negatively impacts phytosterol biosynthesis, plastid pigmentation and leaf growth

Author

Gallón, Sandra M. Navarro, Elejalde-Palmett, Carolina, Daudu, Dimitri, Liesecke, Franziska, Jullien, Frédéric, Papon, Nicolas, de Bernonville, Thomas Dugé, Courdavault, Vincent, Lanoue, Arnaud, Oudin, Audrey, Glévarec, Gaëlle, Pichon, Olivier, Clastre, Marc, St-Pierre, Benoit, Atehortùa, Lucia, Yoshikawa, Nobuyuki, Giglioli-Guivarc’h, Nathalie, and Besseau, Sébastien

Published
2017

4. Co-designing innovative cropping systems that match biophysical and socio-economic diversity : The DATE approach to Conservation Agriculture in Madagascar, Lao PDR and Cambodia

Author

Husson, Olivier, Quoc, Hoá Tran, Boulakia, Stéphane, Chabanne, André, Tivet, Florent, Bouzinac, Serge, Lienhard, Pascal, Michellon, Roger, Chabierski, Stéphane, Boyer, Johnny, Enjalric, Frank, Moussa, Narcisse, Jullien, Frédéric, Balarabe, Oumarou, Rattanatray, Bounmy, Castella, Jean-Christophe, Charpentier, Hubert, and Séguy, and Lucien

Published
2016

5. Biosynthesis of monoterpene scent compounds in roses

Author

Magnard, Jean-Louis, Roccia, Aymeric, Caissard, Jean-Claude, Vergne, Philippe, Sun, Pulu, Hecquet, Romain, Dubois, Annick, Oyant, Laurence Hibrand-Saint, Jullien, Frédéric, Nicolè, Florence, Raymond, Olivier, Huguet, Stéphanie, Baltenweck, Raymonde, Meyer, Sophie, Claudel, Patricia, Jeauffre, Julien, Rohmer, Michel, Foucher, Fabrice, Hugueney, Philippe, Bendahmane, Mohammed, and Baudino, Sylvie

Published
2015

9. Role of petal-specific orcinol O-methyltransferases in the evolution of rose scent (1)

Author

Scalliet, Gabriel, Lionnet, Claire, Le Bechec, Mickael, Dutron, Laurence, Magnard, Jean-Louis, Baudino, Sylvie, Bergougnoux, Veronique, Jullien, Frederic, Chambrier, Pierre, Vergne, Philippe, Dumas, Christian, Cock, J. Mark, and Hugueney, Philippe

Subjects
Methyltransferases -- Chemical properties, Phytochemistry -- Research, Roses -- Physiological aspects, Roses -- Genetic aspects, Biological sciences, Science and technology
Published
2006

14. Agrobacterium-mediated transformation of lavandin (Lavandula x intermedia Emeric ex Loiseleur)

Author

Dronne, Sandrine, Moja, Sandrine, Jullien, Frederic, Berger, Françoise, Caissard, Jean-Claude, Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales (LBVPAM), and Université Jean Monnet [Saint-Étienne] (UJM)

Subjects
lamiaceae, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, ß-glucuronidase, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Agrobacterium tumefaciens, [SDE.IE]Environmental Sciences/Environmental Engineering, transformation, fungi, lavandin, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, neomycin phosphotransferase II
Abstract

International audience; Lavandin (Lavandula x intermedia Emeric ex Loiseleur) is an aromatic plant, the essential oil of which is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. The qualitative or quantitative modification of its terpenes-containing essential oil by genetic engineering could have important scientific and commercial applications. In this study, we report the first Agrobacterium tumefaciens-mediated gene transfer into lavandin. The transformation protocol was optimized by lengthening precultivation and cocultivation periods and by testing five different bacterial strains. We obtained transformed callus lines at a frequency of 40-70% with strains AGL1/GI, EHA105/GI and C58/GI. Transgenic shoots were regenerated from these kanamycin resistant calli and rooted on selective medium with 150 mg l −1 kanamycin. The final percentage of transgenic plants obtained varied from 3 to 9%, according to the Agrobacterium strain used, within 6 months of culture. The presence of the introduced β-glucuronidase and neomycin phosphotransferase II genes was shown both by PCR and Southern blot analysis. Transgene expression was investigated using histoenzymatic β-glucuronidase assays, leaf callus assays and RT-PCR. Results showed that both β-glucuronidase and neomycin phosphotransferase II genes were expressed at a high level in at least 41% of the transgenic plants regenerated. This efficient transformation strategy could be used to modify some genetic traits of lavandin (flower colour, pathogens resistance) and to study the biosynthesis of the major monoterpene components of its essential oil (linalool, linalyl acetate, camphor and 1,8-cineole).

Published
1999
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results