Search

This study aimed to evaluate the supplementation of the probiotic Pseudoalteromonas piscicida 1Ub to the biofloc system as an ecofriendly strategy for protecting white shrimp (Penaeus vannamei) from Vibrio parahaemolyticus infection. Shrimp with an average body weight of (0.50 ± 0.09) g were reared in 30 glass jars with a working volume of 2.5 L at a density of 20 ind/L. Shrimp were reared for 5 d for each treatment, which included the biofloc system without and with 106 colony forming unit (CFU) per mL probiotic. The regular clear water system was used as control. All treatment groups were challenged with 103, 105, and 107 CFU/mL V. parahaemolyticus. For the negative control, shrimp were reared without V. parahaemolyticus. The results showed that the density of V. parahaemolyticus cocultured with P. piscicida 1Ub decreased and the density of V. parahaemolyticus in rearing water and shrimp body in the probiotic-treated group was lower than that in the control group (P

Micro minerals play a crucial role in the physiological processes of fish, acting as enzyme cofactors and stabilizing enzyme and protein structures. One of the essential micro minerals needed in the body is zinc (Zn). Zinc is utilized for various physiological processes, including the maintenance of enzymatic processes within the fish's body. The aim of this literature review is to analyze the optimal level of zinc supplementation in feed to enhance the growth performance of Nile tilapia (Oreochromis niloticus), based on the reported findings of conducted research. The literature sources were obtained from accredited national journals, international journals, research outcomes, theses, and dissertations over the past 20 years. The results of this literature review indicate that zinc supplementation in feed can have a positive impact on overall fish growth by increasing digestive enzyme activity, particularly protease enzymes. The level of zinc supplementation in fish is influenced by several factors including, fish size, feed consumption quantity, and duration of maintenance. The optimal dietary zinc intake for Nile tilapia growth is reported to be in the range of 20–50 mg/kg. Keywords: growth, literature study, Nile tilapia, zinc ABSTRAK Mikro mineral memiliki peran yang sangat penting dalam fisiologi tubuh ikan yang berfungsi sebagai kofaktor enzim dan menjadi penstabil struktur enzim maupun protein. Salah satu mikro mineral yang memiliki fungsi penting dan diperlukan dalam tubuh adalah unsur seng (Zn). Unsur Zn digunakan untuk proses fisiologis termasuk pemeliharaan proses enzimatik dalam tubuh ikan. Tujuan studi pustaka ini adalah menganalisis tingkat suplementasi Zn yang optimum dalam pakan untuk meningkatkan performa pertumbuhan ikan nila Oreochromis niloticus berdasarkan laporan hasil-hasil penelitian yang telah dilakukan. Sumber pustaka didapatkan dari jurnal nasional terakreditasi, jurnal internasional, hasil penelitian, tesis, dan disertasi pada kurun waktu 20 tahun terakhir. Hasil studi pustaka ini menunjukkan bahwa suplementasi Zn dalam pakan dapat memberikan pengaruh positif pada pertumbuhan ikan secara umum melalui peningkatan aktivitas enzim pencernaan terutama enzim protease. Tingkat suplementasi Zn pada ikan dipengaruhi oleh beberapa faktor diantaranya, ukuran ikan, jumlah konsumsi pakan, dan lama pemeliharaan. Nilai asupan Zn yang memberikan pertumbuhan optimum pada ikan nila adalah sebesar 20–50 mg/kg. Kata kunci: ikan nila, pertumbuhan, seng, studi pustaka

This study aimed to evaluate the inhibition of V. parahaemolyticus infection in the whiteleg shrimp (Penaeus vannamei) reared in different volumes of biofloc system. Post-larval shrimp with an average body weight of 0.28 ± 0.01 g were reared in 15 aquariums with working volume of 33.3 L and stocking density of 3 shrimp per liter. The shrimp were reared for 21 days in a biofloc system (C:N ratio of 10) with biofloc volume of 5, 10, and 15 mL/L, and challenged with V. parahaemolyticus at a density of 103 CFU/mL initially. The positive control treatment involved shrimp reared without biofloc and challenged, while the negative control treatment involved shrimp reared without biofloc and without challenged. The shrimp was fed with commercial feed while the protein content of 39-40% four times a day. The results showed that the presumptive Vibrio count (PVC) and the population of V. parahaemolyticus in the biofloc treatments were significantly lower than the positive control (p

The different carbohydrate complexities are thought to affect the bioavailability of carbon sources for heterotrophic bacteria in the biofloc formation process and produce different biofloc characteristics. Based on these, the purpose of this study was to evaluate the effect of using carbon sources with different levels of complexity on the characteristics and quality of biofloc in pacific white shrimp (Penaeus vannamei) rearing. The carbon sources used in this study were dextrose (DX), potato starch (ST), and α-cellulose (CL). The experimental tests were the TAN (total ammonia nitrogen) reduction test which was carried out for 16 hours and the shrimp rearing test which was carried out for 30 days. Parameters measured to determine the characteristics of biofloc in shrimp rearing test were bifloc volume, biofloc particle size, biofloc nutrient composition, and water quality. The DX treatment produced the lowest TAN in the TAN reduction test. The shrimp rearing test showed the biofloc had different nutrient quality between the treatments. Meanwhile, the water quality between the treatments had relatively close values, but the CL treatment was able to suppress TAN and NO2 lower than the DX and ST treatments during the rearing period. In addition, it is feared that the biofloc volume of the DX treatment which is higher than the ST and CL treatments is feared to be the main cause of stress in shrimp. The conclusion of this study is that complex carbohydrates, especially cellulose, have more potential in improving the performance of the biofloc system. Keywords: biofloc volume, carbohydrate complexity, nutrient composition, particle size ABSTRAK Kompleksitas karbohidrat diduga dapat memengaruhi bioavaibilitas sumber karbon bagi bakteri heterotrof dalam proses pembentukan bioflok, sehingga kompleksitas karbohidrat yang berbeda diduga dapat menghasilkan karakteristik bioflok yang berbeda. Berdasarkan hal tersebut maka tujuan dari penelitian ini adalah untuk mengevaluasi pengaruh penggunaan sumber karbon dengan tingkat kompleksitas yang berbeda terhadap karakteristik dan kualitas bioflok pada pemeliharaan udang vaname (Penaeus vannamei). Sumber karbon yang digunakan dalam penelitian ini adalah dekstrosa (DX), tepung kentang (ST), dan α-selulosa (CL) dan rancangan percobaan yang digunakan adalah RAL (rancangan acak lengkap). Penelitian ini memiliki dua jenis pengujian yang dilakukan secara paralel, yaitu uji penurunan TAN (total ammonia nitrogen) yang dilakukan selama 16 jam dan uji pemeliharaan udang yang dilakukan selama 30 hari. Parameter yang diukur untuk mengetahui karakteristik bioflok pada pemeliharaan udang adalah volume biflok, ukuran partikel bioflok, komposisi nutrien bioflok, dan kualitas air pemeliharaan. Perlakuan DX menghasilkan TAN paling rendah pada uji penurunan TAN. Bioflok yang dihasilkan dari uji pemeliharaan udang menunjukkan kualitas nutrien bioflok yang berbeda-beda antar perlakuanya. Sementara itu kualitas air antar perlakuan memiliki nilai yang relatif berdekatan, tetapi perlakuan CL mampu menekan TAN dan NO2 lebih rendah dari perlakuan DX dan ST selama masa pemeliharran. Selain itu, volume bioflok akhir perlakuan perlakuan DX yang lebih tinggi dari perlakuan ST dan CL dikhawatirkan dapat menjadi penyebab utama stres pada udang. Kesimpulan dari penelitian ini adalah karbohidrat kompleks khususnya selulosa lebih berpotensi dalam meningkatkan performa sistem bioflok. Kata kunci: kompleksitas karbohidrat, komposisi nutrisi, ukuran partikel, volume bioflok

Cinnamaldehyde is an active organic compound and an aromatic aldehyde substance found in cinnamon oil. It is a new feed supplement for animals that can improve growth, nutrient digestion and absorption, lipid use, and immunity. The present study evaluated the effects of dietary trans-cinnamaldehyde 98 % (CIN) on nutrient utilization, antioxidant capacity, and growth performance in Nile tilapia (Oreochromis niloticus). Juveniles used was weighing at about 19.77 ± 0.10 g received dietary CIN at different doses (0, 0.25, 0.5, 0.75, and 1.0 g/kg feed). The fish were maintained in a glass aquarium of 95 × 45 × 35 cm3 in size at a stocking density of 20 fish per aquarium and fed three times a day to clear satiation. Dietary carbohydrate digestion and absorption were significantly improved in fish fed 0.75 g/kg CIN, as indicated by increased amylase activity and glucose tolerance test. The latter was subsequently confirmed by increases in relative ir, glut4, hx and gs expression that indicated the increase in glucose absorption led to an increase in glucose utilization as an energy source and the synthesis of glycogen as an energy reserve. Interestingly, dietary CIN supplementation also resulted in the downregulation of fas, which facilitates the conversion of glucose to fatty acids, and the upregulation of cpt1a and hsl, indicating increased fatty acid oxidation for energy. CIN at a range of 0.5–0.75 g/kg improved the antioxidant status in the liver, as illustrated by elevated glutathione peroxidase and superoxide dismutase activities (both P < 0.05). Final weight, feed consumption, the specific growth rate, feed efficiency, and protein retention increased were significantly in all CIN groups, with the highest values reached in the 0.5 g/kg CIN (all P < 0.05). Based on a polynomial orthogonal analysis of the fish-specific growth rate, the optimum dosage of CIN inclusion is 0.42 g/kg.

This study aimed to evaluate the growth performance and carbohydrate metabolism of Pacific whiteleg shrimp after feeding with different cinnamaldehyde concentrations and protein-energy ratios. The study used a completely randomized design with six treatments in triplicates. The treatments were S003213; treatment S053213; treatment S052814; treatment S102814; treatment S052815; and treatment S102815. The study was conducted for 56 days in a 76 L volume aquarium using shrimps with 1.38 ± 0.01 g at 200 individuals/m3. The results showed that the S053213 treatment was significantly different (P

Biofloc technology has been shown to have a positive impact on shrimp culture by controlling pathogenic Vibrio. This study aimed to analyze the effect of biofloc on the Vibrio composition in water and shrimp gut, as well as the growth performance and microvilli of Pacific white shrimp (Penaeus vannamei). Shrimp post-larvae measuring 0.66 ± 0.02 g were reared in a glass aquarium (working volume 22 L) of 6 units with a density of 150 shrimp m-3 for 28 days. The treatments included rearing in the biofloc system with C/N ratio 10, and without a biofloc system as a control. The results showed that Vibrio was highly prevalent in the control gut (4.13%) and biofloc water (3.77%), but only a few were found in the control water (0.16%) and biofloc-treated gut (0.11%). V. hepatarius (1.10%) and V. nereis (1.06%) were found to dominate the Vibrio bacterial community in the biofloc system maintenance media, while Vibrio sp. Hep-1b-8 (2.26%) and V. parahaemolyticus (0.80%) known as a pathogenic bacteria dominated the control shrimp gut. The biofloc system significantly increased the digestive enzyme activity, growth performance, and microvilli length in the shrimp gut. In conclusion, the application of a biofloc system in shrimp culture can affect the composition and abundance of bacterial communities in both the culture environment and the shrimp gut, and improve growth performance with higher digestive enzyme activity and longer microvilli in the gut. Keywords: biocontrol, biofloc, microbiota, shrimp, Vibrio ABSTRAK Teknologi bioflok menunjukkan dampak positif pada kegiatan budidaya udang vaname dengan mengendalikan bakteri patogen salah satunya Vibrio. Penelitian ini bertujuan untuk menganalisis efek bioflok terhadap komposisi Vibrio pada air dan usus udang, serta kinerja pertumbuhan dan mikrovili udang vaname (Penaeus vanamei). Udang post-larva berukuran 0.66 ± 0.02 g dipelihara pada akuarium kaca (volume air 22 L) sebanyak 6 unit dengan kepadatan 150 ekor m-3 selama 28 hari. Perlakuan meliputi pemeliharaan udang pada sistem bioflok dengan rasio C/N 10, dan tanpa sistem bioflok sebagai perlakuan kontrol. Hasil pengamatan menunjukkan kelimpahan Vibrio yang cukup tinggi pada usus perlakuan kontrol (4.13%) dan air perlakuan bioflok (3.77%), tetapi sangat sedikit ditemukan pada air perlakuan kontrol dan usus perlakuan bioflok. Selanjutnya, V. hepatarius (2.26%) dan V. nereis (1.06%) terdeteksi mendominasi komunitas bakteri Vibrio di media pemeliharaan pada sistem bioflok, sedangkan Vibrio sp. Hep-1b-8 (2.26%) dan V. parahaemolyticus (0.80%) mendominasi usus udang vaname pada perlakuan kontrol. Sistem bioflok juga mampu meningkatkan aktivitas enzim pencernaan, performa pertumbuhan, dan panjang mikrovili usus secara signifikan (P < 0.05). Kesimpulan dari penelitian ini adalah penerapan sistem bioflok pada budidaya udang vaname mampu memengaruhi komposisi dan kelimpahan komunitas bakteri Vibrio pada lingkungan budidaya maupun pada usus udang, serta meningkatkan performa pertumbuhan dengan aktivitas enzim pencernaan yang lebih baik dan mikrovilli usus yang lebih panjang. Kata kunci: bioflok, biokontrol, microbiota, udang vaname, Vibrio

Phytoadditives have been acknowledged to have some beneficial effects on metabolic processes and health of some aquaculture species. This study aimed to evaluate the effect of dietary citral on carbohydrate utilization, growth and health performance of vannamei shrimp P. vannamei. This study used a completely randomized design (CRD) with four dietary treatments with different citral doses (0, 50, 75, and 100 mg citral/kg feed) in triplicates. Shrimp were reared for 60 days using fiber containers (50×50×80cm) of 30 shrimp/container. The frequency of feeding is given four times a day. Dietary citral supplementation of 100 mg/kg resulted in higher shrimp growth and feed utilization efficiency than those of the control. The level of amylase enzyme, post-prandial blood glucose, muscle, and liver glycogen were higher in the treatments with dietary citral than those of the controls. The shrimp immune response was also higher in the shrimp fed with dietary citral diets as shown by the higher total hemocyte count, respiratory burst, and phenoloxidase activity compared to those of the control. At the same time, antioxidant capacity in the hepatopancreas as indicated by superoxide dismutase and malondialdehyde level were also improved in shrimp with citral treatment. It can be concluded that dietary citral could increase the utilization carbohydrate, growth performance and health of shrimp at a concentration 75 mg/kg. Keywords: citral, carbohydrate, growth, health, P. vannamei ABSTRAK Secara umum telah diketahui bahwa fitoaditif memiliki pengaruh yang menguntungkan pada proses metabolisme dan kesehatan beberapa spesies akuakultur. Penelitian ini bertujuan untuk mengevaluasi pengaruh penambahan citral pada pakan terhadap pemanfaatan karbohidrat, kinerja pertumbuhan dan kesehatan udang vanname. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan dosis citral yang berbeda (0, 50, 75, dan 100 mg citral/kg pakan) dengan tiga ulangan. Udang vanname dipelihara selama 60 hari menggunakan wadah bak fiber (50×50×80cm) sebanyak 30ekor/wadah. Frekuensi pemberian pakan diberikan sebanyak empat kali sehari. Penambahan citral 100 mg/kg menghasilkan pertumbuhan udang yang lebih tinggi dan efisiensi pemanfaatan pakan dibandingkan kontrol. Tingkat enzim amilase, glukosa hemolim post-prandial, glikogen otot, dan glikogen hati lebih tinggi pada perlakuan penambahan citral dibandingkan kontrol. Respon imun udang juga lebih tinggi pada udang yang diberi penambahan citral seperti yang ditunjukkan oleh jumlah total hemosit, respiratory burst, dan aktivitas fenoloksidase yang lebih tinggi dibandingkan dengan kontrol. Pada saat yang sama, aktivitas antioksidan dalam hepatopankreas udang yang ditunjukkan oleh Superoksida dismutase dan Malondialdehida juga meningkat pada udang dengan perlakuan citral. Dapat disimpulkan bahwa penambahan citral dapat meningkatkan pemanfaatan karbohidrat, pertumbuhan dan kesehatan udang pada konsentrasi 75. Kata kunci: citral, karbohidrat, kesehatan, pertumbuhan, udang vaname

Aeromonas hydrophila is the main causative agent of ulcerative disease in catfish and causes considerable economic losses to Indonesian aquaculture. This study evaluates the prebiotic activity and the effect of feed supplementation of dayak onion simplicia powder (DOSP) on the immune response and survival of catfish infected with A. hydrophila. Five doses (0.20, 0.25, 0.30, 0.35, and 0.40 g/mL) of DOSP were tested in vitro to assess the prebiotic activity score. The results showed that a dose of 0.20 g/mL gave a significantly (P

Applying an external carbohydrate source to stimulate heterotrophic bacteria growth in biofloc systems is a common practice which could be simplified by combining the carbohydrate source with the feed into one pellet. In the current study, such an approach was tested in Pacific white shrimp, Litopenaeus vannamei. Wheat bran (WB) containing non-starch polysaccharides (NSP), a hardly digestible carbohydrate, was mixed with a commercial-like shrimp diet (control; CONdiet) to create a wheat bran rich diet (WBdiet). Shrimp were fed isonitrogenously, resulting in the WBdiet group receiving equal absolute amount of nitrogen but more carbon than the CONdiet group. A digestibility trial was performed in a clear-water recirculating aquaculture system for 35 days, using 6 replicates aquaria per diet. Compared to the CONdiet, shrimp fed with the WBdiet had a reduced overall digestibility and an increased faecal carbon to nitrogen ratio (C:N ratio) from 12 to 20. Following the digestibility trial, a second experiment was performed to monitor the growth performance of the shrimp reared in biofloc systems for 42 days, using three replicate tanks per diet. The dissolved nitrogen species concentration was lower in the tanks fed the WBdiet, while the dissolved carbon concentration was similar between the diets. From a system perspective, similar amounts of nitrogen accumulated across the system compartments with both diets, with maximum 3% total nitrogen loss reached at the end of the experiment. The percentage of carbon loss per kg feed was similar between diets. Overall, diluting the CONdiet with wheat bran and feeding both diets isonitrogenously did not reduce shrimp survival and had minor effect on growth (2% reduction of the specific growth rate). Therefore, incorporating NSP-rich ingredients, such as wheat bran applied as one pellet has a potential to simplify the carbon addition management in a biofloc system.

Aquaponics and biofloc are aquaculture techniques for reducing nitrogenous waste with less water exchange. Aquaponics reduces nitrate (NO3-) through the role of vegetable plants, while biofloc assimilates ammonia (NH4+) through the role of floc-forming bacteria. In this study a collaboration was designed between aquaponics and biofloc called aquabioponics. This study was conducted to evaluate the performance of tilapia production, as well as to observe the dynamics of P, K, Ca, Mg, Fe, Mn, Zn, and Cu minerals formed in the system. This study used a completely randomized design with three treatments and three replications, namely aquaponics (AP), biofloc (BF), and aquabioponics (AB). The AP integrates water in fish tank with vegetable, and does not use additional organic carbon sources. BF uses the addition of an organic carbon source, but does not integrate vegetables. Meanwhile, AB uses the addition of an organic carbon source (half of the dose in BF) and also integrates vegetables. Nile tilapia Oreochromis niloticus with a length of 5.56 ± 0.13 cm and a weight of 5.92 ± 0.47 g totaling 200 individuals were monitored for 60 days in a fiber tank containing 500 L of water in each treatment. The vegetables used in the AP and AB are Pok Choi which is harvested every 30 days. In this study, aquabioponics is a system that produces the best tilapia production performance. The minerals Ca, Mg, Mn, Zn, and Cu are thought to be essential in aquaponics and aquabioponics, while Mg is thought to be essential in bioflocs. Keywords: aquaponic, aquabioponic, biofloc, minerals, Nile tilapia ABSTRAK Akuaponik dan bioflok adalah teknik akuakultur untuk mereduksi limbah nitrogen dengan sedikit pertukaran air. Akuaponik mereduksi nitrat (NO3-) melalui peran tanaman sayuran, sedangkan bioflok mengasimilasi amonia (NH4+) melalui peran bakteri pembentuk flok. Penelitian ini merancang kolaborasi antara akuaponik dan bioflok yang disebut akuabioponik. Penelitian dilakukan untuk mengevaluasi kinerja produksi ikan nila, serta mengamati dinamika mineral P, K, Ca, Mg, Fe, Mn, Zn, dan Cu yang terbentuk di dalam sistem. Penelitian ini menggunakan rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan, yaitu akuaponik (AP), bioflok (BF), dan akuabioponik (AB). AP mengintegrasikan air pemeliharaan ikan dengan penanaman sayur, dan tidak menggunakan penambahan sumber karbon organik. BF menggunakan penambahan sumber karbon organik, namun tidak menggintegrasikan sayuran. AB menggunakan penambahan sumber karbon organik (setengah dari dosis pada BF) dan juga mengintegrasikan sayuran. Ikan nila Oreochromis niloticus dengan panjang 5.56±0.13 cm dan bobot 5.92 ± 0.47 g sebanyak 200 ekor dipelihara selama 60 hari pada bak fiber berisi 500 L air di setiap perlakuan. Sayuran pada AP dan AB adalah Pok Choi yang dipanen setiap 30 hari. Pada penelitian ini, akuabioponik merupakan sistem yang menghasilkan kinerja produksi ikan nila terbaik. Mineral Ca, Mg, Mn, Zn, dan Cu diduga esensial pada akuaponik dan akuabioponik, sedangkan pada bioflok mineral yang diduga esensial adalah Mg. Kata kunci: akuaponik, akuabioponik, bioflok, ikan nila, mineral

Aquaponics is the cultivation of fish and plants in a series of systems, either in one container or in separate containers, by paying attention to the balance between fish, plants, and aquatic microorganisms. One of the obstacles in aquaponic activities is that vegetable growth is not optimal due to a lack of dissolved nutrients, so further research is needed to make better plant growth. This study aimed to evaluate catfish and water spinach growth performance in an aquaponic system by adding AB mix nutrients at different fish stocking densities. This study used a completely randomized design with three differences in stocking densities of catfish (150 fish/m3, 200 fish/m3, and 250 fish/m3) as test animals with four replications where AB mix was added to each treatment. The growth performance of catfish is better in control with a stocking density of 250 fish/m3 and without adding nutrients. The growth performance of catfish decreased with the addition of AB mix nutrients, while the growth of water spinach was better. The growth rate of catfish without adding AB mix nutrients was higher than in other treatments. In contrast, all treatments with the addition of AB mix increased the growth performance of water spinach, with the stocking density of catfish not affecting the growth of water spinach plants. Keywords: aquaponics, water spinach, catfish, growth ABSTRAK Akuaponik merupakan budidaya ikan dan tanaman dalam satu rangkaian sistem, baik dalam satu wadah maupun dalam wadah terpisah dengan memperhatikan keseimbangan antara ikan, tanaman dan mikroorganisme perairan. Salah satu kendala sistem akuaponik adalah pertumbuhan sayuran yang tidak optimal karena kurangnya nutrisi terlarut. Tujuan dari penelitian ini untuk mengevaluasi performa pertumbuhan lele dan kangkung dalam sistem akuaponik dengan penambahan nutrisi AB mix dan pada padat tebar ikan yang berbeda. Penelitian menggunakan rancangan acak lengkap dengan tiga perbedaan padat tebar ikan lele (150 ekor/m3, 200 ekor/m3, dan 250 ekor/m3) sebagai hewan uji sebanyak empat ulangan di mana nutrien AB mix ditambahkan disetiap perlakuan. Kinerja pertumbuhan ikan lele lebih baik pada perlakuan kontrol dengan padat tebar 250 ekor/m3 dan tanpa penambahan nutrisi AB mix. Performa pertumbuhan ikan lele menurun dengan adanya penambahan nutrisi AB mix, sedangkan pertumbuhan tanaman kangkung lebih baik. Laju pertumbuhan ikan lele dengan tanpa penambahan nutrisi AB mix lebih tinggi dibandingkan perlakuan lainnya, sedangkan semua perlakuan dengan penambahan AB mix meningkatkan performa pertumbuhan tanaman kangkung dengan jumlah padat tebar ikan lele tidak berpengaruh pada pertumbuhan tanaman kangkung. Kata kunci: akuaponik, kangkung, lele, pertumbuhan

ABSTRAK Pengamatan parameter kualitas air, tanah, kesehatan dan pertumbuhan merupakan hal yang penting untuk dilakukan dalam budidaya udang vaname. Keterkaitan antar parameter tersebut perlu untuk diketahui dan dianalisis lebih lanjut. Penelitian ini bertujuan untuk melihat keterkaitan antara berbagai parameter kualitas air, kualitas tanah, koefisien teknis budidaya dan parameter kesehatan udang dalam kegiatan budidaya udang dengan nilai sedimen redok potensial yang berbeda. Penelitian ini dilakukan dengan perbedaan nilai sedimen redok potensial yaitu 69,33 ± 14,5 mV, 151,00 ± 8,89 mV, dan 210,00 ± 17,32 mV dengan empat ulangan. Udang sebanyak 25 ekor dengan bobot rata rata 1,37 ± 0,04 g dan padat tebar 144 ekor/m2 digunakan dalam penelitian selama 30 hari. Selama penelitian parameter yang diamati meliputi parameter kualitas air, kesehatan dan pertumbuhan. Parameter kualitas air tidak menunjukkan perbedaaan nyata antar perlakuan kecuali nilai pH dan oksigen terlarut. Pada nilai redok potensial yang rendah nilai pH air cenderung lebih tinggi sedangkan pada nilai redok potensial yang lebih tinggi nilai oksigen terlarut lebih tinggi. Parameter kualtias air yang menunjukkan keterkaitan berdasarkan analisis komponen utama (PCA) dan klaster adalah konduktivitas dan TDS sedangkan parameter kualitas tanah yang saling berkaitan adalah total P, total Fe, total Mn dan total S. Adapun parameter pertumbuhan dan kesehatan udang tidak memenuhi syarat untuk diuji lanjut dengan PCA. Kata kunci: kualitas air, PCA, redok potensial, sedimen, udang putih.

This study was aimed to evaluate the influence of corncob hydrolysis on its crude fiber content, digestibility level, and utilization in the red Nile tilapia diet. This study was performed in two steps, namely hydrolysis and digestibility test. The first study step was enzymatic hydrolysis using 0.4 g/kg cocktail enzyme, followed by chemical hydrolysis using hydrochloric acid (HCl) at different treatments, i.e. concentration, incubation period, and ratio. The second step was designed using a completely randomized design with five treatments, i.e. Rd (reference diet), TJt15% (15% unhydrolyzed corncob), TJt30% (30% unhydrolyzed corncob), TJh15% (15% corncob hydrolysis), and TJh30% (30% corncob hydrolysis). The average weight of tilapia was 15.86 ± 0.19 g/fish. The hydrolyzed corncob meal used for the second study step from the hydrolysis production could reduce 57.53% of crude fiber, 38.15% of NDF fiber fraction, 6.43% of ADF fiber fraction, and 61.96% of hemicellulose. The digestibility test results showed that the hydrolyzed corn cob diet obtained a higher digestibility level, digestive enzyme activity, and blood plasma protein than the unhydrolyzed corncob diet (P

Thus, this research was conducted to evaluate the supplementation of heat-killed Lactobacillus plantarum at higher dosages and investigate the effect of heat-killed L. plantarum supplementation on the challenges of Streptococcus agalactiae. A feeding trial was conducted for 90 (initial average body weight of 12.52–12.69 g) days, while a disease challenge was conducted for 17 days. Dietary treatments were formulated to have a ranging level of heat-killed L. plantarum L-137: (1) control treatment without heat-killed L. plantarum, (2) diet containing 10 mg/kg heat-killed L. plantarum L-137 preparation (LP20, which contains 20% heat-killed L. plantarum L-137), (3) diet containing 20 mg/kg LP20, (4) diet containing 100 mg/kg LP20, and (5) diet containing 250 mg/kg LP20. All the diets were formulated to have equal values of protein and energy. Dietary supplementation of heat-killed L. plantarum L-137 improved tilapia growth performance and higher robustness against S. agalactiae infection. Therefore, a 10–20 mg/kg LP20 feed supplementation level is recommended to support the tilapia growth. In addition, an LP20 dietary supplementation level of 250 mg/kg feed is recommended for higher protection against S. agalactiae.

Cultivation of Moina sp is still constrained by its quality, productivity, and sustainability. The alternative solution is the use of cultivation media materials that have high nutritional content and easily available in large quantities to support the quality and productivity of Moina sp. and meet the needs of live feed. The objective of the study was to evaluate the effect of various culture medium on the productivity and nutritional quality of Moina sp.. Five culture media were tested in laboratory scale, i.e. organic ingredient (BO), Chlorella sp. (Ch), Chlorella sp. + organic ingredients (ChBO), biofloc (BF) and biofloc + organic ingredients (BFBO). While in mass scale, four culture media were tested, i.e. Chlorella sp. (Ch), Chlorella sp. + Organic Ingredients (ChBO), Biofloc (BF) and Biofloc + Organic Ingredients (BFBO). The peaks of Moina sp. density in different treatments were achieved in different days. ChBO treatments significantly had higher productivity (P

This study aimed to evaluate the effect of organic selenium supplementation on diet with different protein levels on the growth performance and protein utilization of African catfish juvenile. A randomized 2×3 factorial design with two dietary protein levels (27% and 32%) and three dietary selenium (Se) supplementation levels (0 mg/kg, 3 mg/kg, and 6 mg/kg diet) in triplicates were applied in the study. African catfish juvenile with an initial average body weight and body length of 27.00 ± 0.14 g and 15.0 ± 0.5 cm, respectively, was reared in 18 units of aquarium (141 L) at a density of 142 fish/m3 for a rearing period of 40 days. Increasing organic Se supplementation level up to 6 mg/kg at high protein feed resulted in higher fish growth and final biomass, lower FCR, and higher protein utilization efficiency than those of other treatments. Furthermore, supplementation of organic Se also resulted in lower lipid and higher Se concentrations in the fish body as well as higher blood protein level compared to those of the control. In conclusion, the result of this study suggested that dietary supplementation of organic Se up to 6 mg/kg could enhance the growth and protein utilization in African catfish fed with both low and high protein diet. Keywords: African catfish, growth, dietary protein, protein utilization, organic selenium. ABSTRAK Penelitian ini bertujuan mengevaluasi pengaruh suplementasi selenium organik pada pakan dengan kadar protein yang berbeda terhadap kinerja pertumbuhan dan pemanfaatan protein pakan ikan lele Clarias gariepenus. Penelitian didesain menggunakan rancangan acak lengkap faktorial 2×3 dengan dua tingkat protein pakan (27% dan 32%) dan tiga tingkat suplementasi selenium (Se) pakan (0 mg/kg, 3 mg/kg, dan 6 mg/kg diet) sebanyak tiga ulangan. Ikan lele yang digunakan memiliki bobot awal rata-rata dan panjang tubuh 27 ± 0.14 g dan 15.0 ± 0.5 cm, dipelihara dalam 18 unit akuarium (141 L) dengan kepadatan 142 ekor/m3 selama 40 hari pemeliharaan. Peningkatan suplementasi Se organik hingga 6 mg/kg pada ikan yang diberi pakan protein tinggi menghasilkan kinerja pertumbuhan ikan dan biomassa akhir yang lebih tinggi, FCR yang lebih rendah, dan efisiensi pemanfaatan protein pakan yang lebih tinggi daripada perlakuan lain. Selain itu, suplementasi Se organik juga menghasilkan kadar lemak yang lebih rendah dan konsentrasi Se tubuh yang lebih tinggi serta kadar protein darah yang lebih tinggi. Kesimpulan dari penelitian ini yaitu suplementasi Se organik pada pakan hingga 6 mg/kg dapat meningkatkan kinerja pertumbuhan dan pemanfaatan protein pakan pada ikan lele yang diberi pakan dengan kadar protein rendah dan tinggi. Kata kunci: ikan lele, pertumbuhan, protein pakan, pemanfaatan protein, selenium organik.

This study was aimed to evaluate the use of corn steep powder (CSP) as a plant protein source in Oreochromis niloticus diet. A commercial feed with 28% protein content and 368 kcal/g energy was used as reference diet, while the test feed consisting of various CSP content, namely 0%, 10%, 20%, and 30% and feed containing soybean meal (SBM) at the level of 20% and 30%. Tilapia were used in the trial with the initial body weight of 6.44 ± 0.29 g, and reared for thirty days in the aquarium at the density of fifteen and fed 3 times daily at a satiation level. All diets were supplied by 0.5% of Cr2O3 as an indicator for digestibility measurement. This study applied the completely randomized design experimental method containing six diet treatments and four replications. The result showed that CSP contains 40.27% protein, 26.10% lactic acid, and minerals. CSP is low in crude fiber and anti-nutritional factors. This study results that increasing the level of CSP significantly decreased feed acidity (P

This study aimed to evaluate the production performance and nitrogen and phosphorus mass balance of biofloc-based intensive African catfish Clarias gariepinus culture at different densities. African catfish with an average body weight of 2.64 ± 0.06 g was randomly distributed into 12 units of round tank with a working volume of 2 m3 of water and maintained for 8 weeks. A completely randomized experimental design with four treatments (in triplicates), i.e. a control treatment at a fish density of 500 fish m-3 with regular water exchange and without organic carbon source addition, and biofloc treatments (BFT) at three different densities, i.e. 500 fish m-3 (BFT500), 750 fish m-3 (BFT750), and 1000 fish m-3 (BFT1000). Biofloc systems were performed with a regular addition of tapioca flour (40% C). The production performance between biofloc system and the control was not significantly different, however water and nitrogen utilizations were significantly more efficient in biofloc system than those of the control. The highest fish specific growth rate was observed in BFT1000 and BFT500 (6.01% day-1 and 5.96% day-1, respectively) (P

Betaine plays some important roles in feed utilization and fish metabolism. The aim of this study was to evaluate the effect of dietary betaine supplementation on the growth performance and feed utilizationin hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀). A completely randomized experimental design with four dietary levels of betaine, i.e.0.0%, 0.5%, 1.0%, and 2.0% in quadruplicate was done.Hybrid grouper juvenile obtained from Brackishwater Aquaculture Development Center, Situbondo, with an initial body length and body weight of 5.89 ± 0.05 cm and 2.86 ± 0.09 g, respectively was used as the tested animal. The fish was maintained in 60 cm x 40 cm x 40 cm aquaria with 75 L working capacity with individual recirculating system with a fish density of 15 fish/aquarium for 50 days. Experimental diet was provided to apparent satiation twice a day. The results of this study demonstrated that dietary betaine at a level of 0.5% resulted in higher feed utilization efficiency, protein and methionine retentions, and growth performance and lower ammonia excretion than those of the control (P

This research aimed to evaluate the health status, growth performance, and meat quality of African catfish Clarias gariepinus maintained in biofloc and nonbiofloc systems. This study applied 3 treatments with 4 replications, i.e. K500 (control, a non-biofloc system at a fish stocking density of 500/m3), BF500 (biofloc system at a fish stocking density of 500/m3), and BF700 (biofloc system at a fish stocking density of 700 /m3). The initial body length and bodyweight of the fish used in this experiment were 10–12 cm and 10–12g, respectively. Feeding was done by using a commercial feed containing 29.76% protein content. The results showed that fish survival in treatments BF500 and BF700 were significantly different (P

The present study aims to evaluate the effect of dietary fulvic acid supplementation on the accumulation of Pb and Cd and the growth performance of Nile tilapia Oreochromis niloticus fed with diets containing green mussel with high Cd and Pb content. Nile tilapia juveniles with a mean body weight of 8.9 ± 0.6 g were randomly distributed into 15 aquarium units (100 L, 17 fish per aquarium). Five experimental diets were formulated to contain 10% mussel meal with high Pb and Cd concentrations, and five levels of fulvic acid, i.e. 0, 100, 200, 400 and 800 mg kg−1 and fed to the fish to the satiation level for 60 days of culture. The consumption of diets containing Pb and Cd resulted in the accumulation of these heavy metals in the fish, however, fulvic acid dietary supplementation could reduce the accumulation of the heavy metals. Furthermore, the dietary supplementation of fulvic acid up to 400 mg kg−1 had significantly improved the fish growth performance and feed utilization. In conclusion, the dietary supplementation of fulvic acid of 200 mg kg−1 could be recommended to prevent Pb and Cd accumulation and to enhance the growth of Nile tilapia fed with diet containing heavy metals.

ABSTRACT This study aimed to evaluate sago pulp fermentation method and its effect on crude fiber content, digestibility, and utilization of sago pulp as a feed raw material for Nile tilapia. Fermentation was done using three different fermenters, i.e. yeast tapai and baker’s yeast with five doses of 10 g/kg, 20 g/kg, 50 g/kg,70 g/kg,100 g/kg, respectively, and sheep rumen liquid with five doses of 100 mL/kg, 200 mL/kg, 300 mL/kg, 500 mL/kg and 1000 mL/kg. The incubation time was 0, 24, 72, and 96 hours. In the digestibility test, tilapia (25 g) was stocked at a density of 7 fish/aquarium. Three different diets were applied in quadruplicate, i.e. reference diet (100% reference diet), unfermented sago pulp (AS), and fermented sago pulp (ASF). Three different dietary treatments (in quadruplicate) containing different carbohydrate sources were tested, i.e. cassava flour as a comparion (G), unfermented sago pulp (AS), and fermented sago pulp (ASF). Fermentation of sago pulp with tapai yeast at a dose of 50 g/kg at 72 hours incubation time could reduce crude fiber by 35.76%, neutral detergent fiber (NDF) by 32.68%, and hemicellulose by 60.39%. Fermentation with yeast tapai could significantly increase sago pulp dry matter digestibility by 34% and carbohydrate digestibility by 21%, as well as increase glucose absorption. The growth experiment showed that the use of ASF diets resulted in higher specific growth rate (3.31 ± 0.12%/ day), protein retention (47.34 ± 5.23%) and fat retention (85.58 ± 5.44%) than those of AS dietary. In conclusion, fermentation of sago pulp using yeast tapai at a dose of 50 g/kg at 72 hours incubation could reduce crude fiber content and increase dry matter and carbohydrate digestibilities, so that it can be used as a source of carbohydrates in tilapia diet. Keywords : carbohydrate, digestibility, fermentation, fiber, Nile tilapia, sago pulp ABSTRAK Penelitian ini bertujuan mengevaluasi metode fermentasi ampas sagu dan pengaruhnya terhadap kandungan serat kasar, kecernaan, dan pemanfaatan ampas sagu sebagai bahan baku pakan ikan nila. Fermentasi dilakukan dengan penambahan tiga perlakuan bahan fermentor yaitu ragi tape dan ragi roti ditambahkan dengan dosis masing-masing sebanyak 10 g/kg, 20 g/kg, 50 g/kg, 70 g/kg, 100 g/kg, dan cairan rumen domba yang ditambahkan dengan dosis 100 mL/kg, 200 mL/kg, 300 mL/kg, 500 mL/kg, dan 1000 mL/kg. Lama waktu inkubasi 0, 24, 72, dan 96 jam. Pada uji kecernaan digunakan ikan nila (25 g) yang dipelihara dengan kepadatan tujuh ekor per akuarium. Pada uji ini dilakukan tiga perlakuan pakan dengan empat ulangan, yaitu pakan acuan, ampas sagu tanpa fermentasi (AS), dan ampas sagu fermentasi (ASF). Percobaan dilakukan dengan tiga perlakuan pakan (4 ulangan) dengan tiga sumber karbohidrat yang berbeda yaitu gaplek (G) sebagai pembanding, ampas sagu (AS), dan ampas sagu fermentasi (ASF). Fermentasi ampas sagu dengan menggunakan ragi tape sebanyak 50 g/kg dengan lama inkubasi 72 jam dapat menurunkan serat kasar tertinggi sebanyak 35.76%, dan menurunkan fraksi serat neutral detergent fiber (NDF) dan hemisellulosa masing-masing sebanyak 32.68% dan 60.39%. Perlakuan fermentasi ampas sagu dapat meningkatkan nilai kecernaan bahan sebesar 34%, kecernaan karbohidrat sebesar 21%, serta penyerapan glukosa. Hasil uji pertumbuhan menunjukkan bahwa perlakuan ASF memberikan nilai laju pertumbuhan spesifik (3.31 ± 0.12%/hari), retensi protein (47.34 ± 5.23%) dan retensi lemak (85.58 ± 5.44%) yang lebih tinggi dibandingkan perlakuan AS (P

ABSTRACT The objective of this research was to determine the optimum level of organic selenium supplementation in improving the growth performance of Nile tilapia Oreochromis niloticus fed with diet containing low protein level. Basal diet was formulated to contain 28% protein with three different levels of organic selenium supplementation, namely 0 (control), 3, and 6 mg Se/kg feed. Furthermore, to evaluate selenium function on protein utilization and sparring effect, a diet with 30% of protein content was also used as a comparison. In this regard, this study was conducted using a completely randomized experimental design with four treatments and three replications. Nile tilapia with an average body weight of 8.05 ± 0.25 g were reared in the 100×50×50 cm3 aquarium at a density of 15 fish/aquarium. The experimental fish were reared for 60 days and fed three times daily to apparent satiation levels. Dietary supplementation of organic Se resulted in higher fish biomass, lower feed conversion ratio, higher protein retention and daily growth rate compared to the control diets with 28% and 30% protein levels. In conclusion, dietary supplementation of organic Se at 3 mg organic Se/kg feed could significantly increase protein utilization and improve the growth performance of Nile tilapia. Keywords: tilapia, protein sparing effect, organic selenium, feed ABSTRAK Tujuan dari penelitian ini adalah menentukan tingkat suplementasi selenium organic pada pakan untuk meningkatkan kinerja pertumbuhan ikan nila Oreochromis niloticus. Pakan basal diformulasikan mengandung 28% protein dengan tiga tingkat selenium organik yaitu 0 (kontrol), 3, dan 6 mg Se/kg pakan. Selanjutnya untuk mengevaluasi penggunaan selenium pada pemanfaatan protein dan efek sparring, pakan dengan 30% protein juga ditambahkan sebagai perlakuan pembanding. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan dan tiga ulangan. Ikan nila dengan bobot rata-rata adalah 8.05 ± 0.25 g dipelihara dalam akuarium 100 × 50 × 50 cm3 dengan kepadatan 15 ikan/akuarium. Ikan dipelihara selama 60 hari dan diberi pakan tiga kali sehari secara at satiation. Pemberian pakan dengan suplementasi Se organic menghasilkan biomassa ikan yang lebih tinggi, rasio konversi pakan yang lebih rendah, retensi protein dan laju pertumbuhan harian yang lebih tinggi daripada perlakuan control dengan kadar protein 28% dan 30%. Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa suplemtasi pakan ikan nila dengan 3 mg Se organik/kg pakan dapat meningkatkan pemanfaatan protein pakan dan kinerja pertumbuhan ikan Nila. Kata kunci: ikan nila, protein sparing effect, selenium organik, pakan.

ABSTRACT Fermentation liquid from mangrove leaves Avicennia marina contains microorganisms, nutrients, and secondary metabolites. This study aimed to identify bacteria and the compounds in fermentation liquid of mangrove leaves A. marina and measured their inhibitory capacity against pathogenic bacteria Stenotrophomonas maltophilia which causes ice-ice disease in seaweed. Molecular analysis which aimed the 16S rRNA gene showed that the bacteria in fermentation liquid consisted of eight types of Bacillus, Bacillus subtilis MSAR-01, Bacillus megaterium MSAR-02, Bacillus firmus MSAR-03, Bacillus thuringiensis MSAR-04, Bacillus subterranerus MSAR-05, Bacillus vietnamensis MSAR-06, Bacillus sp. MSAR-07, Bacillus circulans MSAR-08, with the best inhibitory power indicated by B. subtilis MSAR-01, B. vietnamensis MSAR-06, and Bacillus sp. MSAR-07. The administration of lactic acid, bacteriocin, total fermentation liquid, and supernatant as much as 15 mL produce inhibition to S. maltophilia indicated better result than using one or a combination of several types of bacterial isolates. The inhibition of single bacterial enriched fermentation and supernatant liquids was better than bacterial combination enrichment. Keywords: Avicennia marina, fermentation, ice-ice, mangrove

ABSTRACT The study aimed to evaluate the utilization of seaweed Caulerpa racemosa as feed ingredient for tiger shrimp Penaeus monodon. This research consisted of two different stages, i.e. digestibility and growth test. Tiger shrimp with average body weight of 5.70 ± 0.42 g was reared during digestibility test. The measured parameters were total protein, calsium, magnesium, and energy digestibility. The growth test was managed by using a completely randomized design consisted of four different treatments (in triplicates) of dietary C. racemosa meal addition levels, i.e. 0 (control), 10, 20, and 30%. Tiger shrimp with an average body weight of 0.36 ± 0.02 g were cultured for 42 days in plastic containers (70×45×40 cm) with a stocking density of 15 shrimp/container. Apparent dry matter, protein, calcium, magnesium, and energy digestibilities of C. racemosa were 51.82, 88.67, 68.44, 16.39, 60.30%, respectively. The results presented that the growth performance of tiger shrimp fed with diet containing 10% of C. racemosa was not significantly different with the control (P>0.05). However, the growth performance of the shrimp fed with diet containing more than 20% of C. racemosa decreased. The enzyme activitity of superoxide dismutase (SOD) increased with the higher level of dietary addition of C. racemosa. It can be concluded that C. racemosa was possibly applied up to 10% in the feed formulation for tiger shrimp. Keywords: Caulerpa racemosa, Penaeus monodon, digestibility, growth performance, shrimp ABSTRAK Penelitian ini bertujuan mengevaluasi pemanfaatan rumput laut Caulerpa racemosa sebagai bahan baku pakan udang windu Penaeus monodon. Penelitian ini dilakukan dengan dua tahap, yaitu uji kecernaan C. racemosa dan uji pertumbuhan udang. Udang windu yang digunakan pada uji kecernaan berbobot 5,70 ± 0,42 g. Parameter uji yang diukur meliputi kecernaan total, protein, kalsium, magnesium, dan energi. Uji pertumbuhan dilakukan menggunakan rancangan acak lengkap dengan empat perlakuan dan tiga ulangan, yaitu penggunaan tepung C. racemosa sebesar 0 (kontrol), 10, 20, dan 30%. Udang windu dengan bobot 0,36 ± 0,02 g dipelihara dalam wadah kontainer plastik ukuran 70×45×40 cm (volume air sebanyak 90 L) dengan kepadatan 15 ekor tiap wadah selama 42 hari. Hasil penelitian menunjukkan kecernaan total C. racemosa pada udang windu 51,82%, kecernaan protein 88,67%, kecernaan kalsium 68,44%, kecernaan magnesium 16,39%, dan kecernaan energi 60,30%. Penelitian tahap kedua pada kinerja pertumbuhan udang yang mengonsumsi pakan mengandung 10% C. racemosa, tidak memberikan nilai yang berbeda nyata dengan udang yang mengonsumsi pakan kontrol. Namun, kinerja pertumbuhan udang menurun setelah mengonsumsi pakan yang mengandung C. racemosa di atas 20%, sedangkan aktivitas enzim superoxide dismutase (SOD) meningkat. Dari penelitian ini dapat disimpulkan bahwa penambahan C. racemosa ke dalam formula pakan sampai 10% dapat digunakan sebagai bahan baku pakan udang windu. Kata kunci: Caulerpa racemosa, Penaeus monodon, kecernaan, kinerja pertumbuhan, udang

ABSTRACT Lysozymes play an important role in the first line of defense in fish and potentially used as an immunity status biomarker and immune responses evaluation in fish, which often found in two types, i.e. chicken-type and goose-type (c- and g-type, respectively). To recent, the information related to the sequences and the expression analysis of the c- and g-type lysozyme genes in African catfish is still limited. In the present study, we report a partial cloning and mRNA expression analysis of c-type and g-type lysozymes in African catfish Clarias gariepinus. We have successfully cloned and partially identify the c-type, and g-type lysozyme genes of C. gariepinus, which consist of 594 and 560 of coding sequences, respectively. Catalytic and other conserved residues were identified by multiple sequences alignment and they showed high similarity with other teleost fish species. mRNA levels of the genes were analyzed by using qPCR method and their expressions in the spleen, liver, and head kidney were rapidly modulated after Aeromonas hydrophila injection, with different patterns were observed in each organ. These results confirmed that c- and g-type lysozymes played an important role in non-specific immunity against A. hydrophila infection. This study provided valuable information that can be used to understand the African catfish immune systems for better disease and stress management in C. gariepinus culture. Keywords: lysozymes, gene identification, gene expression, bacterial infection, African catfish ABSTRAK Lisozim berperan dalam sistem pertahanan dini pada ikan dan sangat potensial digunakan sebagai marka status imunitas dalam evaluasi respons imun. Lisozim umum ditemukan dalam dua tipe pada ikan: tipe-ayam (tipe-c) dan tipe-angsa (tipe-g). Informasi terkait sekuens gen dan ekspresi gen kedua tipe lisozim pada ikan lele dumbo sangat terbatas. Pada penelitian ini, kami melaporkan kloning gen secara parsial, dan analisis ekspresi gen dari kedua tipe lisozim pada ikan lele dumbo C. gariepinus. Sekuens parsial gen lisozim tipe-c dan tipe-g yang berhasil diidentifikasi adalah sepanjang 594 dan 560 pasang basa. Situs katalitik dan residu khas memiliki tingkat kesamaan yang tinggi dengan spesies ikan yang lain. Analisis mRNA dilakukan dengan metode quantitative PCR (qPCR). Ekspresi kedua gen di jaringan ginjal depan, limpa, dan hati dengan cepat terinduksi pasca infeksi bakteri A. hydrophila dengan pola yang berbeda. Hasil ini menunjukkan bahwa lisozim tipe-c dan tipe-g memiliki peran yang sangat penting dalam sistem imun ikan lele dumbo terhadap infeksi A. hydrophila. Penelitian ini menghasilkan informasi penting yang dapat digunakan untuk mempelajari sistem imun ikan lele dumbo dan sebagai acuan dalam penanganan dan manajemen penyakit pada budidaya ikan lele dumbo. Kata kunci: lisozim, identifikasi gen, ekspresi gen, infeksi bakteri, ikan lele dumbo

ABSTRACT This study was conducted to evaluate the digestibility of biofloc meal collected from catfish culture as a feed raw material for Pacific white shrimp Litopenaeus vannamei diet. A basal feed with 43% crude protein content was used as a control diet and mixed with 2% of binders and 0.5 % of Cr2O3 as a marker for digestibility. The experimental diets were made by mixing 67.5% of the basal diet with 30% of biofloc meal, 2% of binders and 0.5 % of Cr2O3. Nine units of glass tanks (90 cm ×40 cm× 35 cm) filled with 100 L seawater were used as the experimental culture units. White shrimp with an average body weight of 5.61 ± 0.09 g was randomly distributed to each experimental tank at a density of 20 shrimp/tank. The feed was offered at a level of 5% shrimp biomass per day at a frequency of four times a day. The results showed that the dry matter digestibility of feed with 30% biofloc meal in shrimp were similar to that of the reference diet. However, protein and fat digestibility of feed containing biofloc meal were considerably higher than those of the reference diet. Feeding shrimp with 30% biofloc meal diet resulted in higher survival and specific growth rate and lower feed conversion ratio than those of the control. The digestibility of bioflocs dry matter, protein and lipid in Pacific white shrimp obtained in this study were 54.9%, 76.3% and 79.3%, respectively. Keywords: biofloc, digestibility, catfish, shrimp ABSTRAK Penelitian ini dilakukan untuk mengevaluasi kecernaan tepung bioflok yang diambil dari media pemeliharaan ikan lele sebagai bahan pakan udang vaname Litopenaeus vannamei. Pakan yang mengandung kadar protein 43% digunakan sebagai pakan control dan dicampur dengan 2% binder dan 0.5% Cr2O3 sebagai marker untuk kecernaan. Pakan perlakuan dibuat dengan mencampurkan 67.5% pakan control dengan 30% tepung bioflok, 2% binder dan 0.5% Cr2O3. Penelitian menggunakan sembilan unit akuarium (90 cm ×40 cm ×35 cm) yang diisi 100 L air laut. Udang vaname dengan bobot rata-rata 5.61 ± 0.09 g ditebar secara acak pada setiap akuarium perlakuan pada kepadatan 20 ekor/akuarium. Pakan diberikan dengan tingkat pemberian pakan 5% biomassa per hari sebanyak empat kali sehari. Hasil penelitian ini menunjukkan bahwa kecernaan pakan dengan 30% tepung bioflok tidak berbeda nyata dengan pakan acuan. Namun kecernaan protein dan lemak pakan yang mengandung tepung bioflok terlihat lebih tinggi daripada pakan kontrol. Pemberian pakan dengan tepung bioflok sebanyak 30% juga menghasilkan tingkat kelangsungan hidup, laju pertumbuhan spesifik udang dan rasio konversi pakan yang lebih baik daripada udang yang diberi pakan kontrol. Kecernaan bahan, protein dan lemak tepung bioflok pada udang yang didapat dalam penelitian masing-masing adalah 54.9%, 76.3% dan 79.3%. Kata-kata kunci: bioflok, ikan lele, kecernaan, udang

The supply of land-based agricultural products as aquafeed raw materials is challenged by limitations on space and water, and by environmental damage. Marine environments offer a vast opportunity for the expansion of aquaculture, including the production of feed raw materials. Besides fishmeal and fish oil, which are generated from capture fisheries, the use of marine-based feed raw materials from aquaculture production is not yet in common practice. Here, we discuss the potential of underutilized marine organisms that can be cultured by extracting nutrients from their environment and are nutritionally compatible for use as alternative feed materials in aquaculture. We identify marine organisms such as blue and green mussels, Ulva spp., and microbial floc that are nutritionally suitable as aquafeed raw material and may further act as bioremediators. However, environmental factors that affect productivity and the risk of pollutant accumulations, which would potentially reduce the safety of aquaculture products for human consumption, may pose challenges to such applications of extractive organisms. Therefore, the development of pretreatment and processing technologies will be critical for improving the nutritional quality and safety of these raw materials for aquafeed production.

ABSTRACTThis study aimed to evaluate the growth performance and resistance to salinity stress of striped catfish juvenile reared in biofloc with different feeding rate (FR). The treatments applied in this study were rearing the fish in biofloc with FR 5% and 8% of biomass per day, and rearing the fish with a FR of 8% per day without biofloc system as the control. The fish with an initial average length of 1.81 ± 0.20 cm were stocked in 9 units of 50 L aquaria with density of 40 ind/aquaria (800 ind/m3) for 15 days rearing period. In biofloc systems, the addition of tapioca as a source of organic carbon was done every day with an estimated C/N ratio of 10. No water exchange was done in biofloc systems, whereas regular water exchange was applied in the control. Results of the experiment showed that survival was not significantly different amongst treatments (P>0.05). However, the specific growth rate of the fish in biofloc system with a FR of 8% per day showed the highest value and was significantly different from other treatments (P Keywords: biofloc, feeding rate, growth, salinity stress test, striped catfish ABSTRAKPenelitian ini bertujuan untuk mengevaluasi kinerja pertumbuhan dan ketahanan benih ikan patin terhadap stres salinitas yang dipelihara dalam sistem bioflok dengan tingkat pemberian pakan (FR) berbeda. Perlakuan yang terdapat dalam penelitian ini adalah benih patin yang dipelihara dalam sistem bioflok dengan FR 5% dan 8% per hari, dan pemeliharaan benih dengan FR 8% per hari tanpa penambahan sumber karbon sebagai kontrol. Benih patin dengan panjang rata-rata awal 1,81 ± 0,20 cm dipelihara dalam 9 unit akuarium dengan volume air 50 L dan kepadatan 40 ekor/akuarium (800 ekor/m3) selama 15 hari. Pada sistem bioflok, penambahan tapioka sebagai sumber karbon dilakukan setiap hari dengan C/N 10. Pada sistem bioflok tidak dilakukan pergantian air, sedangkan pada kontrol dilakukan pergantian air. Kelangsungan hidup ikan tidak berbeda nyata antar perlakuan. Namun, tingkat pertumbuhan spesifik ikan dalam sistem bioflok dengan FR 8% per hari menunjukkan nilai tertinggi dan berbeda nyata antar perlakuan (P0,05). Uji stres salinitas dilakukan dengan merendam 15 ekor benih patin pada air dengan salinitas 20 g/L selama satu jam. Kelangsungan hidup setelah uji stres salinitas dari benih yang dipelihara di bioflok secara signifikan lebih tinggi dibandingkan kontrol (P Kata kunci: bioflok, feeding rate, pertumbuhan, uji stres salinitas, ikan patin

ABSTRACTThis study was aimed to evaluate the utilisation of biofloc meal collected from biofloc-based catfish intensive culture as a mix ingredient for Nile tilapia Oreochromis niloticus and common carp Cyprinus carpio diet. A control diet containing 29.03% crude protein was used in this experiment. Experimental diet was made by mixing 30% biofloc waste meal with the control diet and repelleted after the addition of 2% of binder. To determine the experimental feed digestibility, 0.5% of Cr2O3 was added as a marker for digestibility. The feed was offered to satiation at a frequency of 3 times a day for 28 days of experimentation. Nile tilapia and common carp juveniles with an initial average body weight of 11.72±0.04 g and 8.81±0.04 g, respectively, were used as the experimental animals. Each fish species were randomly stocked with a density of 10 fish/aquarium (30´45´30 cm3). The results showed that dry matter digestibility of diets with 30 % biofloc waste meal in both fish species were significantly lower than those of the controls (PKeywords: biofloc meal, digestibility, growth performance, tilapia, common carp ABSTRAK Penelitian ini bertujuan untuk mengevaluasi pemanfaatan tepung bioflok yang dikumpulkan dari limbah pemeliharaan ikan lele intensif berbasis teknologi bioflok sebagai campuran pakan untuk ikan nila Oreochromis niloticus dan ikan mas Cyprinus carpio. Pakan kontrol yang digunakan adalah pakan komersial dengan kadar protein 29,03%. Pembuatan pakan uji dilakukan dengan mencampurkan tepung limbah bioflok (30%) dengan pakan kontrol (67,5%) dan dibentuk pelet kembali setelah dilakukan penambahan binder sebanyak 2% dan Cr2O3 sebanyak 0,5% sebagai penanda untuk menganalisis kecernaan pakan dengan tepung limbah bioflok. Pakan diberikan secara at satiation dengan frekuensi pemberian pakan 3 kali/hari selama 28 hari pemeliharaan. Bobot rata-rata ikan awal adalah 11,72±0,04 g untuk ikan nila, dan 8,81±0,04 g untuk ikan mas dengan kepadatan awal masing-masing 10 ekor/akuarium (30´45´30 cm3). Hasil penelitian ini menunjukkan bahwa nilai kecernaan total pakan dengan tepung limbah bioflok baik pada ikan nila maupun ikan mas lebih rendah daripada pakan kontrol (P0,05). Kata kunci: tepung limbah bioflok, kecernaan, kinerja pertumbuhan, ikan nila, ikan mas

ABSTRACT This research was conducted to evaluate the effect of different Artemia feeding period on the size variation of catfish Clarias sp. larvae. One thousand two days post hatched larvae with an average body length of 0.7±0.03 cm were randomly distributed into 25 L round plastic tanks. The treatment conducted for 13 days with four treatment; without Artemia, given Artemia 1, 2, or 3 days in the first culture period. The results showed that 15 days old fish has the same survival rate in all treatments; and has two size distribution i.e. the small size (S) and medium size (M). Increasingly the period of administration of Artemia, the percentage of the amount of M-size fish increases, thereby giving Artemia were able to increase growth of larvae. Feeding catfish with Artemia for two days has shown producing better growth. Keywords: catfish, Artemia, size variation, growth performance ABSTRAK Penelitian ini dilakukan untuk mengevaluasi pengaruh perbedaan periode pemberian pakan Artemia terhadap kinerja pertumbuhan larva ikan lele Clarias sp. Seribu ekor larva lele yang berumur dua hari dengan panjang rata-rata 0,7±0,03 cm ditebar dalam wadah dengan volume air 25 L. Budidaya ikan dilakukan selama 13 hari dengan empat perlakuan, yaitu perlakuan tanpa pemberian Artemia, pemberian Artemia selama satu, dua, atau tiga hari di awal masa budidaya. Hasil penelitian menunjukkan bahwa ikan umur 15 hari memiliki sintasan yang sama di semua perlakuan, serta memiliki dua sebaran ukuran, yakni ukuran kecil (S) dan ukuran sedang (M). Semakin lama periode pemberian Artemia, persentase jumlah ikan ukuran M meningkat, sehingga pemberian Artemia pada larva ikan lele mampu meningkatkan pertumbuhan. Pemberian Artemia selama dua hari memberikan pertumbuhan yang lebih baik. Kata kunci: ikan lele, Artemia, keragaman ukuran, kinerja pertumbuhan

Organic carbon source and C/N ratio play an important role in aquaculture system with biofloc technology application. Nitrogen control by adding carbohydrates to the water to stimulate heterotrophic bacterial growth by converting nitrogen into bacterial biomass. The study investigated the effect molasses, tapioca, tapioca by product and rice bran as carbon sources in a biofloc media at three different C/N ratios i.e. 10, 15, and 20 on total ammonia reduction in biofloc media. Five liters of biofloc media in a conical tank was prepared for each replicate, which consisted of 500 mL of biofloc suspension collected from a shrimp culture unit with biofloc technology application and 4.5 L seawater. Pacific white shrimp culture was performed in 40L glass aquaria at a shrimp density of 30/aquarium. There was a significant interaction between carbon source and the C/N ratio applied (P

ABSTRACT Freshwater prawn Macrobrachium rosenbergii is one of the most important aquaculture commodities in Indonesia. This study evaluated the effect of dietary β-glucan supplementation (0%, 0.075%, 0.15%, and 0.225%) on the immune parameters, survival, and growth of giant freshwater prawn. Prawn juvenile with an initial body weight of 1.25±0.041 g was randomly distributed into eight units of glass aquaria (150 L) with a density of 16 animals/aquarium. Phenoloxydase activity, total and differential haemocyte counts, survival, and specific growth rate of the prawn were measured and calculated on the final day of experiment (day 42). Phenoloxydase activity, total and differential haemocyte counts of β-glucan treatments were higher than control, whereas no significant difference was observed in survival. The highest specific growth rate was observed in the 0.15% β-glucan supplementation treatment with 1.92%/day. Keywords: phenoloxydase activity, haemocyte, β-glucan, specific growth rate, giant freshwater prawn ABSTRAK Udang galah Macrobrachium rosenbergii merupakan salah satu komoditas akuakultur yang penting di Indonesia. Penelitian ini dilakukan untuk mengevaluasi pengaruh suplementasi pakan dengan β-glukan sebesar 0%, 0,075%, 0,15%, dan 0,225% terhadap parameter imunitas dan kinerja pertumbuhan udang galah. Juvenil udang dengan bobot tebar rata-rata 1,25±0,041 g ditebar secara acak pada delapan unit akuarium (150 L) dengan padat tebar 16 ekor/akuarium. Parameter yang diamati adalah aktivitas fenoloksidase (PO), jumlah dan diferensiasi hemosit, sintasan, dan laju pertumbuhan spesifik yang diukur dan dihitung setelah 42 hari masa pemeliharaan. Hasil penelitian menunjukkan bahwa nilai aktivitas PO, total hemosit, dan diferensial hemosit pada udang yang diberi pakan dengan suplementasi β-glukan cenderung lebih tinggi dibandingkan dengan kontrol. Sintasan masing-masing perlakuan tidak berbeda nyata dengan perlakuan kontrol. Laju pertumbuhan spesifik tertinggi terdapat pada perlakuan β-glukan 0,15% sebesar 1,92%/hari. Kata kunci: aktivitas phenoloxydase, hemosit, β-glukan, udang galah, laju pertumbuhan spesifik

This study aimed to evaluate the utilization of smoked skipjack processing byproduct meal (SPBM) to reduce the use of fish meal (FM) for juvenile humpback grouper Cromileptes altivelis. This study consisted of digestibility test of SPBM and biological test to observe growth performance. Five isonitrogenous and isocaloric experimental diets were used: Diet A contains 0% SPBM as a control diet, and diets B, C, D, and E contain 25%, 50%, 75%, and 100% of SPBM protein to substitute FM, respectively. Digestibility trial was performed for 14 days by adding Cr2O3 into the experimental diets and collecting fecal matter 40–60 minutes after each feeding. For growth trial, juvenile humpback grouper were kept in glass aquariums and fed by the experimental diet until apparent satiation for 60 days. Our result shows that the dry matter and protein SPBM apparent digestibility coefficient is lower compared to FM. The fish that was fed with diet B and C performed a comparable specific growth rate, feed efficiency, and protein retention compared to control (p > 0.05). However, lower specific growth rate, feeding efficiency, and protein retention were observed in fish that was fed with diet D (75% SPBM) and diet E (100% SPBM; p

ABSTRACT This research was aimed to examine the growth performance and non-specific immune response of Pacific white shrimp (Litopenaeus vannamei) fed on the diet supplemented with β-(1,3) glucan (BG) and poly-β-hydroxybutyrate (PHB) as feed additives. Shrimp juvenile at an initial body weight of 2.06±0.03 g was randomly distributed into 12 units of aquaria at a density of 20 shrimps/tank and reared for 42 days. The treatments applied in this study were control (without feed additives), 1.5 g/kg BG, 10 g/kg PHB and 1,5 g/kg BG+10 g/kg PHB. Results showed that shrimp fed on 1.5 /kg BG-supplemented feed had significantly higher growth performance and non-specific immune response. Keywords: growth, shrimp, non-specific immune response, Litopenaeus vannamei ABSTRAK Penelitian ini bertujuan untuk menguji kinerja pertumbuhan dan respons imun nonspesifik udang vaname Litopenaeus vannamei yang diberi pakan dengan penambahan feed additive berupa β-(1,3) glukan (BG) dan poli-β-hidroksibutirat (PHB). Juvenil udang 2,06±0,03 g dipelihara pada 12 unit akuarium dengan empat perlakuan dan tiga ulangan, serta padat tebar 20 ekor/tank selama 42 hari pemeliharaan. Perlakuan yang diberikan dalam penelitian ini yaitu penambahan BG (1,5 g/kg), PHB (10 g/kg), dan BG (1,5 g/kg)+PHB (10 g/kg), serta kontrol (tanpa penambahan feed additive). Hasil penelitian menunjukkan bahwa udang yang diberi 1,5 g/kg BG memiliki kinerja pertumbuhan dan respons imun nonspesifik yang terbaik. Kata kunci: pertumbuhan, udang, respons imun nonspesifik, Litopenaeus vannamei

ABSTRACTThe objective of the present experiment was to study the most optimum taurine enrichment concentration of rotifers in improving Pacific white shrimp larva Litopenaeus vannamei survival and development. White shrimp larvae at sixth naupliar stage were reared in 12 units of 500 L fibre glass tanks with a stocking density of 125 ind/L. Starting from zoea two stage (Z-2), the larva was provided with rotifers with different taurine enrichment concentration according to the treatments, i.e. 0 mg/L enrichment medium (A), 25 mg/L (B), 50 mg/L(C), and 100 mg/L (D). The results show that different taurine concentration in the enrichment media increased taurine level in rotifers. Furthermore, the administration of taurine enriched rotifers up to 50 mg/L significantly improved larval survival and may accelerate larval development. The experimental results also concluded that a concentration of 50 mg/L is the most optimum taurine enrichment concentration of rotifers for the improvement of white shrimp larval survival and developmental stage.Keywords: taurine, rotifer, white shrimp, enrichmentABSTRAKPenelitian ini bertujuan untuk mengkaji konsentrasi optimum taurin melalui pengayaan pada rotifera terhadap tingkat kelangsungan hidup dan perkembangan stadia larva udang vaname Litopenaeus vannamei. Larva udang vaname stadia naupli-6 dipelihara dalam 12 tangki fiberglass volume 500 L dengan kepadatan 125 ind/L. Dimulai sejak stadia zoea 2 (Z-2) larva diberi rotifera yang diperkaya dengan taurin dengan konsentrasi yang berbeda sesuai dengan perlakuan, yaitu 0 mg/L media pengkaya (A), 25 mg/L (B), 50mg/L (C), dan 100mg/L (D). Hasil penelitian menunjukkan pengayaan taurin pada konsentrasi yang berbeda menyebabkan peningkatan kandungan taurin rotifera. Sementara pemberian rotifera yang diperkaya taurin hingga 50 mg/L meningkatkan kelangsungan hidup dan mempercepat perkembangan stadia larva udang. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa pemberian rotifera yang diperkaya taurin dengan konsentrasi 50 mg/L menghasilkan tingkat kelangsungan hidup tertinggi (53,5%) dan perkembangan larva yang tercepat (pKata kunci: taurin, rotifera, udang vanname, pengayaan

ABSTRACTThe objective of this study was to study the effect of incubation period on the nutritional content of Azolla sp. meal fermented by Trichoderma harzianum, and its optimum supplementation level in the feed of tilapia Oreochromis sp. In incubation period treatments, fermentation of Azolla meal was performed in two, six, eight, and ten days (AF2, AF6, AF8, AF10) using Trichoderma harzianum as the fermentor. The fish used in this study was tilapia Oreochromis sp. with an average weight of 10.59±1.29 g. The design of the feeding treatments was repeletting commercial feed with Azolla leaves by with different supplementation levels, i.e. 0% (A/control), 30% (B), 60% (C), and 90% (D). Faecal collection for digestibility measurement was conducted for 15 days and fish growth rate was observed for 40 days. Azolla meal fermented for two days (AF2) showed the best results among the other treatments with a crude fiber decrease of 37.19% and protein increase of 38.65%. The results of this study indicate that fermentation can increase the nutritional quality of Azolla meal and its most optimal supplementation level in the diet of tilapia is 30%.Keywords: crude fiber, Azolla sp., tilapiaABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh lama waktu fermentasi daun mata lele Azolla sp. menggunakan kapang Trichoderma harzianum serta dosis optimal dalam pakan ikan nila Oreochromis sp. Proses fermentasi tepung daun mata lele dilakukan selama dua, enam, delapan, dan sepuluh hari (AF2, AF6, AF8, AF10). Ikan uji pada penelitian ini menggunakan ikan nila Oreochromis sp. dengan bobot rata-rata 10,59±1,29 g yang ditebar sebanyak 6 ekor/akuarium berukuran 50×45×30 cm3. Sebagai pakan perlakuan yakni repeletting daun mata lele dengan pakan komersil pada tingkat suplementasi 0% (A/kontrol), 30% (B), 60% (C), dan 90% (D). Pemeliharaan ikan uji dan pengumpulan data dilakukan dengan mengumpulkan feses ikan untuk uji ketercernaan selama 15 hari dan mengamati pertumbuhan ikan selama 40 hari. Tepung daun mata lele yang difermentasi selama dua hari (AF2) memiliki hasil yang paling baik di antara perlakuan lainnya yakni dengan penurunan serat kasar sebesar 37,19% dan peningkatan protein sebesar 38,65%. Hasil penelitian ini menunjukkan bahwa fermentasi dapat meningkatkan kualitas nutrisi daun mata lele serta dosis optimal pemanfaatan tepung daun mata lele fermentasi dalam pakan ikan nila adalah sebesar 30%.Kata kunci: serat kasar, Azolla sp., ikan nila

ABSTRACT Two experiments were conducted to evaluate the effect of sheep rumen liquor enzyme addition on the reduction of cocoa-pod husk meal (CPHM) fiber content, and the digestibility of hydrolyzed CPHM for tilapia Oreochromis niloticus. In the first trial, sheep rumen liquor enzyme was added with various concentration, i.e. 0, 50, 100 and 150 mL/kg CPHM with three different incubation periods, namely 0, 12, and 24 hours. In the second trial, digestibility was determined by the addition of Cr2O3 as the indicator in both reference and experimental diets, i.e. feed with hydrolyzed CPHM and unhydrolyzed CPHM. Tilapia with an average body weight of 3.86±0.44 g were stocked at a density of 15 fish/aquarium and were maintained for 15 days. In the first trial, CPHM hydrolyzed with 150 mL/kg and incubated for 12 and 24 hour showed the lowest crude fiber content (21.38% and 21.67%). Apparent digestibility coefficient of hydrolyzed CPHM was 33.95%, which was higher than unhydrolyzed CPHM (10.97%). As conclusions sheep rumen liquor enzyme addition was effective to decrease crude fiber content of CPHM and improve the apparent digestibility coefficient of CPHM for tilapia diet. Keywords: sheep rumen liquor enzyme, cocoa-pod husk meal, digestibility, tilapia ABSTRAK Dua tahap penelitian dilakukan untuk mengevaluasi penambahan enzim cairan rumen domba dalam menurunkan kandungan serat kasar kulit buah kakao (KBK) dan mengevaluasi ketercernaan KBK yang telah dihidrolisis dengan enzim cairan rumen domba dalam pakan ikan nila Oreochromis niloticus. Pada penelitian tahap satu, enzim cairan rumen domba ditambahkan dengan konsentrasi yang berbeda yaitu 0, 50, 100, dan 150 mL/kg KBK dengan lama inkubasi yang berbeda yaitu 0, 12, dan 24 jam. Pada penelitian tahap dua, nilai ketercernaan ditentukan dengan menggunakan indikator Cr2O3 yang ditambahkan ke dalam pakan acuan dan pakan perlakuan, yaitu pakan dengan penambahan KBK yang telah dihidrolisis dengan dosis terbaik pada penelitian tahap satu (KBKe) dan kulit buah kakao tanpa hidrolisis (KBK). Ikan nila yang digunakan mempunyai bobot rata-rata 3,86±0,44 g ditebar dengan kepadatan 15 ekor/akuarium dan dipelihara selama 15 hari. Hasil penelitian tahap satu menunjukkan hidrolisis KBK dengan menggunakan cairan rumen 150 mL/kg dan lama waktu inkubasi 12 jam dan 24 jam mempunyai nilai serat kasar KBK terendah yaitu sebesar 21,38% dan 21,67%. Pada uji ketercernaan terlihat bahwa nilai ketercernaan bahan KBKe lebih tinggi (33,95%) dibandingkan dengan KBK (10,97%). Dengan demikian dapat disimpulkan bahwa penambahan enzim cairan rumen domba dapat menurunkan kandungan serat kasar kulit buah kakao dan meningkatkan ketercernaan kulit buah kakao pada pakan ikan nila. Kata kunci: enzim cairan rumen domba, kulit buah kakao, ketercernaan, ikan nila

The aim of this research was evaluating the quality and digestibility of fermented local feedstuff as well as its effect on growth performance of common carp Cyprinus carpio juvenile. The local feedstuffs tested in this experiment were kapok seed, cassava peel, copra, rubber seed, and palm kernel meal. The previously milled feedstuff was fermented with instant yeast Saccharomyces cerevisiae with a dose of 0.9% w/w and incubated for 24 hours. Following this, the fermented feedstuffs were dried, mixed with reference diet with a ratio of 3:7 and supplemented with 0.5% Cr2O3 as digestibility test indicator. Common carp juveniles with an initial average body weight of 14,11±1,28 g were cultured for 30 days. Fish feces were collected since the 6th day of culture, and followed by laboratory analyses. The results show that fermentation could increase the crude protein content of feedstuff with a range of 16.85‒31.11%, and decrease crude fiber with a range of 2.45‒31.65% with the exception of copra. Furthermore, fermentation also increased the feed digestibility including protein, energy, and total digestibility by the tested fish, as it is shown that the use of fermented feedstuffs may increase protein digestibility 3.88‒11.73%, 2,21‒10,24%, and 3,63‒72,37%. Finally, it can be concluded that fermentation can increase the digestibility of feed with local ingredients by common carp juvenile. Keywords: fermentation, digestibility, local materials, common carp

This study evaluated the effect of biofloc technology (BFT) application on water quality and production performance of red tilapia Oreochromis sp. at different stocking densities. Three different fish densities were applied, i.e. 25, 50, and 100 fish/m3, and for each density there were Control (without external carbon input) and BFT treatments. Mixed sex red tilapia with an initial average body weight 77.89 ± 3.71 g was cultured in 3 m3 concrete tanks for 14 weeks. Molasses was added on BFT treatments as the organic carbon source at a C/N ratio of 15. Control treatments of each density tested showed more fluctuated water quality parameters throughout the experimental period. The highest TAN and nitrite-nitrogen were observed in control treatment at a stocking density of 100 fish/m3 (3.97 mg TAN/L and 9.29 mg NO2-N/L, respectively). The highest total yield was observed in control treatment at the highest density treatment (43.50 kg), whereas the highest survival was obtained by BFT treatment at a density of 25 fish/m3 (97.78 ± 0.77%). Total feed used in BFT was lower than that of control treatments in particular at 50 fish/m3 density (P < 0.05) suggesting that biofloc could be continuously harvested by the fish as other source of food.

Application of bioflocs technology and probiotics has improved water quality and production of Pacific white shrimp (Litopenaeus vannamei) culture. This experiment was to verify the effect of probiotic bacteria addition and different carbon:nitrogen (C:N) ratio on water quality and performance of Pacific white shrimp nursery culture. Nursery culture was carried out for 25 days in an aquarium under laboratory condition with stock density of one Post-Larvae (PL) (poslarval) per liter (24 PL/aquarium) of PL16 shrimp. Different C:N ratio resulted a significant difference on shrimp production performance. Treatment of 10 C:N ratio demonstrated the best shrimp growth (20.37 ± 0.48% per day in weight and 6.05 ± 0.41% per day in length), harvesting yield (1180 ± 62 g/m3) and feed efficiency (121 ± 6%). There was however no significant difference observed between treatments in water quality.

Application of bio-flocs technology (BFT) in aquaculture offers a solution to avoid environmental impact of high nutrient discharges and to reduce the use of artificial feed. In BFT, excess of nutrients in aquaculture systems are converted into microbial biomass, which can be consumed by the cultured animals as a food source. In this experiment, upconcentrated pond water obtained from the drum filter of a freshwater tilapia farm was used for bio-flocs reactors. Two carbon sources, sugar and glycerol, were used as the first variable, and two different levels of salinity, 0 and 30 ppt, were used as the second variable. Bio-flocs with glycerol as a carbon source had higher total n-6 PUFAs (19.1 ± 2.1 and 22.3 ± 8.6 mg/g DW at 0 and 30 ppt, respectively) than that of glucose (4.0 ± 0.1 and 12.6 ± 2.5 mg/g DW at 0 and 30 ppt). However, there was no effect of carbon source or salinity on crude protein, lipid, and total n-3 PUFAs contents of the bio-flocs.

Bacterial disease is one of the most common diseases in aquaculture practices which have a significant impact. Several researches noted that pathogenicity of a certain bacteria can be determined by its quorum sensing activity. Quorum sensing is a communication process of a certain bacteria with the same or different species of bacteria which involves the releasing and capturing of signal molecule to and from the environment. This activity will activate a certain target gene which further resulted in the expression of a phenotype by the bacteria. With regard to this characteristic, one of the methods to control bacterial diseases is by quorum sensing disruption. Several species of algae, both micro and macro, have been found to be able to intervense bacterial quorum sensing and thus can be used as an alternative in bacterial disease control. Key words: quorum sensing, bacterial disease, aquaculture, algae Abstrak Penyakit bakteri adalah salah satu penyakit yang paling umum dalam akuakultur dengan dampak yang cukup signifikan. Beberapa penelitian menunjukkan bahwa tingkat patogenitas suatu bakteri salah satunya ditentukan oleh aktivitas kuorum sensing bakteri. Kuorum sensing bakteri merupakan suatu proses komunikasi yang dilakukan oleh bakteri dengan bakteri lainnya baik yang sejenis maupun berlainan jenis yang berupa pelepasan dan penangkapan molekul sinyal menuju dan dari lingkungan sekitar bakteri tersebut. Aktivitas inilah yang akan menentukan ekspresi suatu gen target seperti patogenitas, sehingga salah satu metode yang dapat digunakan dalam mengendalikan penyakit yang disebabkan oleh bakteri adalah dengan mengganggu aktivitas kuorum sensing bakteri. Beberapa jenis alga, baik mikro maupun makro, diketahui dapat mengintervensi aktivitas kuorum sensing, dan dapat menjadi salah satu alternatif bagi pengendalian penyakit bakterial. Kata-kata kunci: kuorum sensing, penyakit bakterial, akuakultur, alga

ABSTRACTThe objective of this experiment was to study microbial abundance and diversity in the water, and immune parameters of red tilapia Oreochromis sp. cultured in bioflok system with different fish stocking densities. The experiment comprised of two different factors, carbon source addition (bioflocs and control), and fish stocking density (25 fish/m3, 50 fish/m3, dan 100 fish/m3), with an experimental period of 99 days. Microbial load in water was determined biweekly, whereas immune parameters represented by fish blood profile were measured on day 0, 50, and 90. There was no significant difference in total bacteria count in the water of all treatments; there was however a tendency shown by all treatments that the microbial load in water increased along with the culture period. There were 4 genera of bacteria which particularly found in bioflok system, which are Acinetobacter sp., Corynobacterium sp., Listeria sp., dan Pseudomonas sp, and are suggested to play a role in bioflok formation. The percentage of phagocytic index of fish in bioflok system was higher than that in control, and may indicate that bioflok may stimulate the fish immune system.Keywords: bioflocs, red tilapia, bacteria, blood profile. ABSTRAKPenelitian ini bertujuan untuk mengidentifikasi kelimpahan dan keragaman jenis bakteri dalam air dan parameter imunitas ikan nila Oreochromis sp. yang dipelihara dalam sistem bioflok dengan kepadatan ikan yang berbeda. Penelitian terdiri atas dua faktor perlakuan yaitu penambahan sumber carbon (bioflok dan kontrol), dan padat penebaran ikan (25 ekor/m3, 50 ekor/m3, dan 100 ekor/m3) dengan lama waktu pemeliharaan ikan selama 99 hari. Kelimpahan bakteri diukur setiap 2 minggu sekali selama masa pemeliharaan. Parameter imunitas meliputi gambaran darah diukur dengan pengambilan contoh darah yang dilakukan pada tiga ekor ikan pada hari ke 0, 50, dan 99. Kelimpahan bakteri pada semua perlakuan pada setiap titik pengamatan tidak menunjukkan perbedaan yang nyata. Semua perlakuan menunjukkan kecenderungan peningkatan kelimpahan bakteri seiring dengan masa pemeliharaan. Terdapat 4 genus bakteri yang hanya ditemukan pada kolam bioflok yaitu Acinetobacter sp., Corynobacterium sp., Listeria sp., dan Pseudomonas sp yang diduga berperan dalam pembentukan bioflok. Persentase indeks fagositik pada ikan dengan perlakuan bioflok lebih tinggi dibanding kontrol, yang mengindikasikan peran bioflok sebagai stimulus sistem imun.Kata kunci: bioflok, nila merah, bakteri, gambaran darah.

Bioflocs technology (BFT) is one of the developing technology in aquaculture which aimed to improve water quality and to enhance nutrient utilization efficiency. This technology is mainly based on the conversion of inorganic nitrogen in particular ammonia by heterotrophic bacteria into microbial biomass which further can be consumed by aquaculture organisms. The objective of this review is to discuss various aspect of BFT application in aquaculture including bioflocs formation process, technical requirement, bioflocs nutritional content and characterization techniques. Keywords: bioflocs, nitrogen, heterotrophic bacteria, ammonia, C/N ratio ABSTRAK Teknologi bioflok (BFT) merupakan salah satu teknologi yang saat ini sedang dikembangkan dalam akuakultur yang bertujuan untuk memperbaiki kualitas air dan meningkatkan efisiensi pemanfaatan nutrient. Teknologi ini didasarkan pada konversi nitrogen anorganik terutama ammonia oleh bakteri heterotrof menjadi biomassa mikroba yang kemudian dapat dikonsumsi oleh organisme budidaya. Tujuan penulisan makalah ini adalah untuk memaparkan berbagai aspek dalam BFT termasuk proses pembentukan bioflok, persyaratan teknis, kandungan nutrisi bioflok dan teknik karakterisasinya. Kata kunci: bioflok, nitrogen, bakteri heterotrof, ammonia, rasio C/N

ABSTRACTExperiment was performed to assess the effect of 17a-methyltestosterone (MT) treatment on Congo tetra fish larvae. To evaluate the optimal pattern of MT treatment, three different treatments were administrated. Three months old larvae were submerged in three different doses of MT; 1, 2 and 4 mg/l. These studies showed that the highest percentage of male fish was obtained by 4 mg/l MT treatment, 87,17%. The 2 mg/l and 1 mg/l MT treatments obtained 77,53% and 69,86% male respectively, two times higher than control, 38,96%. On the other hand, the 4 mg/l MT treatment also resulted the highest percentage of hermaphrodite fishes, 17,58%. The highest survival rate was shown by 1 mg/l MT treatment, 62,77% and the lowest was shown by the 4 mg/l MT treatment, 47,20%. The highest rate of fish length and weight was shown by the 4 mg/l MT treatment, 4,4 cm and 1,65 gram respectively. These findings suggest that MT treatment offers an advantage in growth of tetra Congo larvae. Key word : Sex reversal, methyltestosterone, Congo tetra fish, Micraleptus intterruptus. ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh perendaman larva di dalam larutan hormon 17a-metiltestosteron pada dosis 1, 2 dan 4 mg/l larutan. Persentase tertinggi ikan jantan dihasilkan oleh perlakuan 4 mg/l, yaitu 87,17%. Perlakuan 2 mg/l dan 1 mg/l masing-masing menghasilkan 77,53% dan 69,86% sedangkan kontrol menghasilkan 38,96% jantan. Efek lain dari perlakuan MT ini adalah hermafroditisme. Perlakuan 4 mg/l menghasilkan persentase hermafrodit tertinggi yaitu 17,58%, sedangkan pada kontrol kelangsungan hidup tertinggi diperoleh pada perlakuan 1 mg/l (62,77%) dan terendah pada perlakuan 4 mg/l (47,20%). Hasil tersebut menunjukkan adanya pengaruh dosis hormon terhadap kelangsungan hidup ikan. Pengukuran bobot dan panjang ikan pada setiap perlakuan menunjukkan nilai tertinggi dihasilkan oleh perlakuan 4 mg/l yaitu 1,65 gram dan 4,40 cm. Hal ini diduga bahwa hormon MT merangsang pula pertumbuhan ikan.Kata kunci : Pergantian kelamin, metiltestosteron, ikan tetra Kongo, Micraleptus intterruptus.