1. Implications on the Pb bioaccumulation and metallothionein levels due to dietary and waterborne exposures: The Callinectes danae case
- Author
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Bruno Galvão de Campos, Julia Reali Costa Melo, José Roberto Machado Cunha da Silva, Denis Moledo de Souza Abessa, Paloma Kachel Gusso-Choueri, Andrews Krupinski Emerenciano, Isabella C. Bordon, Déborah Inês Teixeira Fávaro, Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), and Centro do Reator de Pesquisa (CRPq)
- Subjects
Gills ,Male ,0106 biological sciences ,Gill ,Muscle tissue ,Callinectes ,Brachyura ,Health, Toxicology and Mutagenesis ,Hepatopancreas ,Artificial food ,010501 environmental sciences ,01 natural sciences ,Acclimatization ,Blue crabs ,Toxicity Tests ,medicine ,Animals ,Metallothionein ,0105 earth and related environmental sciences ,biology ,Chemistry ,Muscles ,010604 marine biology & hydrobiology ,Public Health, Environmental and Occupational Health ,General Medicine ,biology.organism_classification ,PROTEÍNAS ,Pollution ,Crustacean ,Bioaccumulation ,Metallothionein-like proteins ,medicine.anatomical_structure ,Lead ,Metals ,Environmental chemistry ,Water Pollutants, Chemical ,Biomarkers - Abstract
Made available in DSpace on 2018-12-11T17:21:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-10-30 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This study aimed to assess the bioaccumulation of Pb and induction of metallothionein-like proteins (MT) in Callinectes danae through single and combined dietary and waterborne exposures. Male C. danae individuals were collected in the south area of the Cananéia-Iguape-Peruíbe Protected Area (APA-CIP), in São Paulo State, Brazil. After an acclimatization period, exposure assays were performed during 7 and 14 days, at two Pb concentrations (0.5 e 2.0 µg/g) in 4 treatments: 1) control; 2) contaminated water only; 3) contaminated food only; 4) contaminated water and food. The results indicate that C. danae is highly tolerant to Pb exposure at the evaluated concentrations. In gills, Pb bioaccumulation is more dependent of water efflux and time of exposure (higher Pb values). However, pathways act simultaneously in the induction of MT expression in this tissue. The decreases in Pb accumulation in the combined treatments and MT increases after 14 days in gills suggests that these proteins play a detoxification function in the presence of Pb. In hepatopancreas, depending on the predominance of a certain pathway or combined pathways, accumulation occured at different times. For muscle tissue, bioaccumulation was observed due to contaminated water exposure, but not dietary exposure, probably because Pb concentrations were low. UNESP - Univ Estadual Paulista Campus do Litoral Paulista Núcleo de Estudos em Poluição e Ecotoxicologia Aquática (NEPEA), Praça Infante Dom Henrique s/n° Parque Bitaru, CEP 11330-900 USP ‐ Univ. de São Paulo Instituto de Ciências Biomédicas (ICB)- Departamento de Biologia Celular e do Desenvolvimento, Avenida Professor Lineu Prestes, 1524 - Cidade Universitária, CEP 05508-900 IPEN ‐ Instituto de Pesquisas Energéticas e Nucleares Centro do Reator de Pesquisa (CRPq), venida. Professor Lineu Prestes 2242 - Cidade Universitária, CEP: 05508-000 UNESP - Univ Estadual Paulista Campus do Litoral Paulista Núcleo de Estudos em Poluição e Ecotoxicologia Aquática (NEPEA), Praça Infante Dom Henrique s/n° Parque Bitaru, CEP 11330-900 FAPESP: 2014/01576-6
- Published
- 2018