6 results on '"Julia Molle"'
Search Results
2. Using DNA origami nanorulers as traceable distance measurement standards and nanoscopic benchmark structures
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Egbert Buhr, Mario Raab, Bernd Bodermann, Detlef Bergmann, Ija Jusuk, Julia Molle, Philip Tinnefeld, and Harald Bosse
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0301 basic medicine ,Nanostructure ,Materials science ,Microscope ,lcsh:Medicine ,Nanotechnology ,02 engineering and technology ,Article ,law.invention ,03 medical and health sciences ,Nanoruler ,law ,Microscopy ,Calibration ,DNA origami ,lcsh:Science ,Nanoscopic scale ,Fluorescent Dyes ,Multidisciplinary ,lcsh:R ,DNA ,Reference Standards ,021001 nanoscience & nanotechnology ,Nanostructures ,Benchmarking ,030104 developmental biology ,Microscopy, Fluorescence ,Measurement uncertainty ,lcsh:Q ,0210 nano-technology - Abstract
In recent years, DNA origami nanorulers for superresolution (SR) fluorescence microscopy have been developed from fundamental proof-of-principle experiments to commercially available test structures. The self-assembled nanostructures allow placing a defined number of fluorescent dye molecules in defined geometries in the nanometer range. Besides the unprecedented control over matter on the nanoscale, robust DNA origami nanorulers are reproducibly obtained in high yields. The distances between their fluorescent marks can be easily analysed yielding intermark distance histograms from many identical structures. Thus, DNA origami nanorulers have become excellent reference and training structures for superresolution microscopy. In this work, we go one step further and develop a calibration process for the measured distances between the fluorescent marks on DNA origami nanorulers. The superresolution technique DNA-PAINT is used to achieve nanometrological traceability of nanoruler distances following the guide to the expression of uncertainty in measurement (GUM). We further show two examples how these nanorulers are used to evaluate the performance of TIRF microscopes that are capable of single-molecule localization microscopy (SMLM).
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- 2018
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3. The quality of German - language patient decision aids for oncological patients on the internet
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Julia Möller, Lena Josfeld, Christian Keinki, Nathalie Zieglowski, Jens Büntzel, and Jutta Hübner
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Patient decision aids ,Patient information ,Shared-decision-making ,Decision-making process ,Therapy decision ,Medical screening ,Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
Abstract Background Previous studies have already shown that decision aids are a suitable tool for patient decision-making. The aim of this work is to conduct an online search for freely available, German-language patient decision aids (PDAs) for cancer patients, followed by an assessment of their quality. For this purpose, a rating tool that is as manageable as possible was developed on the basis of already existing quality criteria. Methods A simulated patient online search was conducted via the four most frequently used search engines in Germany. A quality assessment tool was created utilizing international and national guidelines, with a focus on practicality and manageability. Subsequently, the identified PDAs were rated by 4 raters based on the rating tool. Results The number of German-language oncology PDAs is low (n = 22 of 200 URLs) with limited variability regarding rare cancers. Most originate from non-profit organizations. The overall quality is low, as indicated by an average of 57.52% of the maximum evaluation points of the developed quality assessment tool. Reference values used to assess quality were related to e.g. support/effectiveness, adaptation, layout, etc. No qualitative differences were found regarding different publishers. Quality differed between PDAs of different length, with longer PDAs achieving better results. Conclusion Overall, the supply and quality of German-language PDAs is not satisfactory. The assessment tool created in this study provides a solid, but more manageable basis, for developing and identifying high-quality PDAs. Practice implications PDAs should be increasingly used by physicians in practice. For this, a quick qualitative assessment of PDAs in everyday life must be possible. Future research has to investigate especially the aspect of the length of a PDA in more detail.
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- 2023
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4. Superresolution microscopy with transient binding
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Philip Tinnefeld, Mario Raab, Dina Grohmann, Daniel Schmitt-Monreal, Zhike He, Susanne Holzmeister, and Julia Molle
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0301 basic medicine ,Chemistry ,Resolution (electron density) ,Biomedical Engineering ,Bioengineering ,Nanotechnology ,DNA ,Ligand (biochemistry) ,Superresolution ,Fluorescence ,3. Good health ,03 medical and health sciences ,030104 developmental biology ,Microscopy, Fluorescence ,Microscopy ,Biophysics ,Molecule ,Transient (oscillation) ,Fluorescent Dyes ,Biotechnology - Abstract
For single-molecule localization based superresolution, the concentration of fluorescent labels has to be thinned out. This is commonly achieved by photophysically or photochemically deactivating subsets of molecules. Alternatively, apparent switching of molecules can be achieved by transient binding of fluorescent labels. Here, a diffusing dye yields bright fluorescent spots when binding to the structure of interest. As the binding interaction is weak, the labeling is reversible and the dye ligand construct diffuses back into solution. This approach of achieving superresolution by transient binding (STB) is reviewed in this manuscript. Different realizations of STB are discussed and compared to other localization-based superresolution modalities. We propose the development of labeling strategies that will make STB a highly versatile tool for superresolution microscopy at highest resolution.
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- 2016
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5. Towards structural biology with super-resolution microscopy
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Leonhard Jakob, Philip Tinnefeld, Mario Raab, Julia Molle, Dina Grohmann, and Johann Bohlen
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0301 basic medicine ,Diffraction ,Materials science ,Super-resolution microscopy ,Resolution (electron density) ,Nanotechnology ,DNA ,Fluorescence ,03 medical and health sciences ,030104 developmental biology ,Förster resonance energy transfer ,Microscopy, Fluorescence ,DNA nanotechnology ,Microscopy ,Fluorescence microscope ,Fluorescence Resonance Energy Transfer ,General Materials Science ,Fluorescent Dyes - Abstract
Fluorescence resonance energy transfer (FRET) has been instrumental in determining the structure and dynamics of biomolecules but distances above 8 nanometers are not accessible. However, with the advent and rapid development of super-resolution (SR) microscopy, distances between two fluorescent dyes below 20 nanometers can be resolved, which hitherto has been inaccessible for fluorescence microscopy approaches due to the limited resolving power of an optical imaging system that is determined by the fundamental laws of light diffraction (referred to as the diffraction limit). Therefore, the question arises whether SR microscopy can ultimately close the resolution gap between FRET and the diffraction limit and whether SR microscopy can be employed for the structural interrogation of proteins in the sub-20 nm range? Here, we show that the combination of DNA nanotechnology and single-molecule biochemistry allows the first step towards the investigation of the structural organization of a protein via SR microscopy. Limiting factors and possible future directions for the full implementation of SR microscopy as a structural tool are discussed.
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- 2018
6. Behavioral Observations in Northern UGANDA: Development of a Coding System to Assess Mother–Child Interactions in a Post-war Society
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Julia Möllerherm, Elizabeth Wieling, Regina Saile, Marion Sue Forgatch, Frank Neuner, and Claudia Catani
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behavioral observations ,structured observation ,post-conflict setting ,mother-child dyads ,cultural adaptation ,parenting ,Psychology ,BF1-990 - Abstract
There is growing interest in causes and consequences of disruptions in parent-child relationships in post-war environments. Recent studies mainly relied on self-reports to gain information about family dynamics following war exposure. Considering the limitations of self-report measures, we see the need for an in-depth examination of post-conflict parenting based on observational and quantitative data. The aim of the present study was the development of a coding system for a culturally bound description of parent–child interactions in northern Uganda, where virtually the entire population has been severely affected by 20 years of civil war. Interactions of 101 mothers and their 6- to 12-year-old children were observed during a structured interaction task (problem solving discussion). Foundation for the development of the coding system was the Family and Peer Process Code (FPP code). The cultural adaptation of the FPP code was based on in-depth qualitative analyses of the problem solving task, including a combination of inductive and deductive latent content analyses of textual data and videotapes, member checking and consultations of experts in the field of behavioral observations. The final coding system consists of 35 exhaustive and mutually exclusive content codes including codes for verbal, vocal, and compliance behavior as well as 14 affect codes. Findings indicate that the assessment of behavioral observations in post-conflict settings provides unique insights into culture- and context-specific interaction patterns and may be critical for the development and evaluation of parenting interventions.
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- 2019
- Full Text
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