Search

Your search keyword '"Julia Helena Oliveira de Barros"' showing total 3 results
Start Over Author "Julia Helena Oliveira de Barros"
3 results on '"Julia Helena Oliveira de Barros"'

1. Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow

Author

Rosana de Almeida Santos, Karina Dutra Asensi, Julia Helena Oliveira de Barros, Rafael Campos Silva de Menezes, Ingrid Rosenburg Cordeiro, José Marques de Brito Neto, Tais Hanae Kasai-Brunswick, and Regina Coeli dos Santos Goldenberg

Subjects
menstrual blood-derived mesenchymal stromal cells, bone marrow mesenchymal stromal cells, human umbilical vein endothelial cells, angiogenesis, Biology (General), QH301-705.5, Chemistry, QD1-999
Abstract

Several therapies are being developed to increase blood circulation in ischemic tissues. Despite bone marrow-derived mesenchymal stromal cells (bmMSC) are still the most studied, an interesting and less invasive MSC source is the menstrual blood, which has shown great angiogenic capabilities. Therefore, the aim of this study was to evaluate the angiogenic properties of menstrual blood-derived mesenchymal stromal cells (mbMSC) in vitro and in vivo and compared to bmMSC. MSC’s intrinsic angiogenic capacity was assessed by sprouting and migration assays. mbMSC presented higher invasion and longer sprouts in 3D culture. Additionally, both MSC-spheroids showed cells expressing CD31. mbMSC and bmMSC were able to migrate after scratch wound in vitro, nonetheless, only mbMSC demonstrated ability to engraft in the chick embryo, migrating to perivascular, perineural, and chondrogenic regions. In order to study the paracrine effects, mbMSC and bmMSC conditioned mediums were capable of stimulating HUVEC’s tube-like formation and migration. Both cells expressed VEGF-A and FGF2. Meanwhile, PDGF-B was expressed exclusively in mbMSC. Our results indicated that mbMSC and bmMSC presented a promising angiogenic potential. However, mbMSC seems to have additional advantages since it can be obtained by non-invasive procedure and expresses PDGF-B, an important molecule for vascular formation and remodeling.

Published
2020
Full Text
View/download PDF

2. Human Menstrual Blood-Derived Mesenchymal Cells Improve Mouse Embryonic Development

Author

Regina Coeli Dos Santos Goldenberg, Karina Dutra Asensi, Anna Luiza Lima Nascimento, Tania M. Ortiga-Carvalho, Marcel Frajblat, Julia Helena Oliveira de Barros, Rosana de Almeida Santos, Tais Hanae Kasai-Brunswick, Marianna Ferreira Gonçalves, and Cherley Borba Vieira de Andrade

Subjects
Stromal cell, media_common.quotation_subject, 0206 medical engineering, Biomedical Engineering, Embryonic Development, Bioengineering, 02 engineering and technology, Biology, Biochemistry, Angiopoietin-2, Biomaterials, Endometrium, 03 medical and health sciences, Humans, Cells, Cultured, Menstrual blood, 030304 developmental biology, media_common, 0303 health sciences, Hepatocyte Growth Factor, Mesenchymal stem cell, Embryogenesis, Cell Differentiation, Mesenchymal Stem Cells, Embryo culture, Embryo, Mammalian, 020601 biomedical engineering, Fibronectins, Cell biology, Blastocyst, Culture Media, Conditioned, Female, Reproduction
Abstract

There is a constant need for improving embryo culture conditions in assisted reproduction. One possibility is to use mesenchymal stem/stromal cells derived from menstrual blood (mbMSCs), with an endometrial origin. In this study, we sought to analyze the expansion of mouse embryos in a direct coculture model with mbMSCs. Our results showed that after five passages, mbMSCs presented a spindle-shaped morphology, with surface markers that were comparable with the normal mesenchymal cell phenotype. mbMSCs could differentiate into adipogenic and osteogenic lineages and secrete angiopoetin-2 and hepatocyte growth factor. The coculture experiments employed 103 two-cell-stage embryos that were randomly divided into two groups: control (

Published
2020

3. Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow

Author

Karina Dutra Asensi, Rosana de Almeida Santos, José Marques de Brito Neto, Tais Hanae Kasai-Brunswick, Rafael Campos Silva de Menezes, Regina Coeli Dos Santos Goldenberg, Ingrid Rosenburg Cordeiro, and Julia Helena Oliveira de Barros

Subjects
CD31, Angiogenesis, Neovascularization, Physiologic, Bone Marrow Cells, Chick Embryo, Biology, menstrual blood-derived mesenchymal stromal cells, Article, Catalysis, lcsh:Chemistry, Inorganic Chemistry, angiogenesis, Paracrine signalling, Cell Movement, In vivo, medicine, Animals, Humans, Physical and Theoretical Chemistry, lcsh:QH301-705.5, Molecular Biology, Cells, Cultured, Spectroscopy, Cell Proliferation, human umbilical vein endothelial cells, Migration Assay, Organic Chemistry, Mesenchymal stem cell, Cell Differentiation, Mesenchymal Stem Cells, bone marrow mesenchymal stromal cells, General Medicine, Chondrogenesis, Immunohistochemistry, Computer Science Applications, medicine.anatomical_structure, lcsh:Biology (General), lcsh:QD1-999, Cancer research, Female, Bone marrow
Abstract

Several therapies are being developed to increase blood circulation in ischemic tissues. Despite bone marrow-derived mesenchymal stromal cells (bmMSC) are still the most studied, an interesting and less invasive MSC source is the menstrual blood, which has shown great angiogenic capabilities. Therefore, the aim of this study was to evaluate the angiogenic properties of menstrual blood-derived mesenchymal stromal cells (mbMSC) in vitro and in vivo and compared to bmMSC. MSC`s intrinsic angiogenic capacity was assessed by sprouting and migration assays. mbMSC presented higher invasion and longer sprouts in 3D culture. Additionally, both MSC-spheroids showed cells expressing CD31. mbMSC and bmMSC were able to migrate after scratch wound in vitro, nonetheless, only mbMSC demonstrated ability to engraft in the chick embryo, migrating to perivascular, perineural, and chondrogenic regions. In order to study the paracrine effects, mbMSC and bmMSC conditioned mediums were capable of stimulating HUVEC&rsquo, s tube-like formation and migration. Both cells expressed VEGF-A and FGF2. Meanwhile, PDGF-B was expressed exclusively in mbMSC. Our results indicated that mbMSC and bmMSC presented a promising angiogenic potential. However, mbMSC seems to have additional advantages since it can be obtained by non-invasive procedure and expresses PDGF-B, an important molecule for vascular formation and remodeling.

Published
2020

Catalog

Books, media, physical & digital resources
See catalog results