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Genetic polymorphisms could explain the inter-individual differences in the oral health-related quality of life (OHRQoL) of children with anterior open bite (AOB). Objective: To assess the impact of AOB on OHRQoL in children and to evaluate whether MTR (rs1805087), MTRR (rs1801394), TGFβ1 (rs1800469) and TNF-α (rs1799964, rs1799724 and rs1800629) genes are potential biomarkers for OHRQoL in children with AOB. Study design: A cross-sectional study was performed with 173 children aged between 2–6 years. The Brazilian version of Early Childhood Oral Health Impact Scale (ECOHIS) was applied. Genetic polymorphisms were analyzed using real-time PCR. Mann-Whitney U-test and Chi-square were used. Results: The overall mean ECOHIS scores were 5.49 (SD= 5.72) and 3.45 (SD = 4.49) (p < 0.01) in the AOB and control groups, respectively. Children with the CC genotype of TNF-α (rs1799724) had a significantly higher psychological QoL level. The MTRR AA genotype group showed a lower QoL level in the child subscale (p = 0.006), function (p = 0.017), and psychological (p = 0.006) domains. There was no significant difference between OHRQoL and the genetic polymorphisms in MTR and TGFβ1. Conclusions: Genetic polymorphisms in TNF-α and MTRR are associated with the impact on the OHRQoL in children with AOB.

A mordida aberta anterior (MAA) merece destaque ímpar, já que além de ser a mais frequente entre as discrepâncias verticais e comprometer a estética dental, interfere também na função mastigatória e na fala do paciente, afetando consequentemente a sua auto- -estima. Por seu caráter multifatorial, essa má oclusão pode ser causada por diversas razões, tais como a herança genética ou desenvolvimento maxilofacial inerente a cada individuo. O presente trabalho teve como objetivo descrever o tratamento de um paciente com MAA. O paciente BS de 7 anos e 5 meses de idade possuía má oclusão de Classe I com mordida aberta anterior (-2,8 mm). O plano de tratamento foi dividido em duas fases: na primeira, foi utilizado o aparelho de Haas com grade lingual na arcada superior; na arcada inferior, utilizou-se um expansor removível. Na sequência do tratamento, instalou-se o aparelho fixo em ambas as arcadas. O tratamento teve duração de 9 (nove) meses e conseguiu o fechamento da mordida aberta anterior. A segunda fase iniciou-se após a irrupção dos segundos molares permanentes e teve duração de 13 meses. Como conclusão se observou que o tratamento da mordida aberta anterior em duas fases foi uma excelente escolha para o tratamento do paciente aqui relatado. Na primeira fase, a mordida foi fechada e as arcadas foram alargadas para permitir o posicionamento adequado de todos os dentes permanentes, que tiveram seu posicionamento perfeitamente detalhado na segunda fase.

Objectives To explore the association between genetic polymorphisms in vitamin D receptor (VDR), vitamin D serum levels, and variability in dental age. Material and methods This cross-sectional study was based on an oral examination, panoramic radiograph analysis, and genotype analysis from biological samples. Dental age was evaluated using two different methods: Demirjian et al. (Hum Biol 45:211–227, 1973) and Hofmann et al. (J Orofac Orthop.78:97–111, 2017). The genetic polymorphisms BglI (rs739837) and FokI (rs2228570) in VDR were genotyped through real-time PCR. The vitamin D level was also measured in the serum. Delta (dental age–chronological age) was compared among genotypes in VDR in the co-dominant model. Multiple linear regression analysis was also performed. An established alpha of 5% was used. Results Genotype distributions of BglI and FokI were not associated with dental maturity (p > 0.05). In the logistic regression analyses, genotypes in BglI and FokI and vitamin D levels were not associated with variability in dental age (p > 0.05). Conclusions The genetic polymorphisms BglI and FokI in VDR and the vitamin D levels were not associated with variability in dental age. Clinical relevance To unravel the factors involved in dental maturity can improve dental treatment planning in pediatric and orthodontic practice.

Zebrafish (Danio rerio, formerly named Brachydanio rerio) is an established model organism used in health research for a long time. In the past decades, some research groups have been using zebrafish model to understand the genes involved in tooth development. Objectives: To introduce zebrafish as an animal model for tooth development researchers and to highlight the advantages and limitations of this model. Literature review: Tooth development (also known as odontogenesis) is a complex process that relies on precise control of several critical regulatory pathways. Because of the complex development and morphogenesis of teeth, many human developmental dental anomalies arise due to disruptions during tooth development. The knowledge regarding signaling pathways and genes expressed during tooth development has mainly been discovered using mice (not modified and genetically modified) and other rodents. Zebrafish (Danio rerio, formerly named Brachydanio rerio) has also been used by some recent researchers. Results: Zebrafish as a model organism has several advantages, and for which a large and diverse set of genetic and molecular tools are already available. Zebrafish has an easy husbandry system and a relatively fast embryonic development. Conclusion: This review provides some insights for the use of zebrafish in understanding tooth development and developmental dental alterations for clinical and basic researchers alike.

Avaliar se existe correlação entre maturação esquelética e maturação dentária em crianças brasileiras. Material e métodos: A amostra foi composta por 37 documentações ortodônticas de pacientes que iniciaram o tratamento ortodôntico para correção da maloclusão classe II na Universidade Positivo. Incluíram-se no estudo documentações de pacientes com idade variando de 10 a 16 anos e de ambos os sexos. Excluíram-se documentações de pacientes com alterações sistêmicas, histórico de traumatismos na região da face, fissuras labiopalatinas e agenesias dentárias. O método de avaliação da maturação mão-punho e o método de avaliação da maturação vertebral cervical serviram para análise da maturação esquelética. Os métodos de avaliação dos estágios de desenvolvimento dentário de Demirjian e Hofmann foram utilizados para avaliação da maturação dentária e o coeficiente de correlação de Pearson, para determinar a força de correlação entre as variáveis. O nível de significância usado foi de 5%. Resultados: A idade cronológica demonstrou correlação moderada com o método de avaliação da maturação esquelética de mão-punho, o método de avaliação da maturação esquelética vertebral cervical e a maturação dentária (r = 0,525, p = 0,003; r = 0,450, p = 0,014; r = 0,564, p = 0,004 e r = 0,436, p = 0,011, respectivamente). O método de avaliação da maturação mão-punho evidenciou correlação forte com o método de avaliação da maturação vertebral cervical (r = 0,864, p

Objective: To evaluate the correlation between the Body Mass Index (BMI) and dental development in patients with Class II malocclusion. Materials and Methods: This was a retrospective study with a sample of 37 patients presenting Class II malocclusion, who were undergoing orthodontic treatment. The age, gender, weight and height data were collected from the medical records, together with the initial CT scan. Dental development was assessed using the method of Demirjian et al. (1973). Pearson’s correlation coefficient was used to determine the correlation strength between variables. The statistical significance considered was p

Objective To investigate SNPs in bone‐ and cartilage‐related genes and their interaction in the aetiology of sagittal and vertical skeletal malocclusions. Settings and sample population This study included 143 patients and classified as follows: skeletal class I (n = 77), class II (n = 47) and class III (n = 19); maxillary retrusion (n = 39), protrusion (n = 52) and well‐positioned maxilla (n = 52); mandibular retrognathism (n = 50), prognathism (n = 50) and well‐positioned mandible (n = 43); normofacial (n = 72), dolichofacial (n = 55) and brachyfacial (n = 16). Materials and methods Steiner's ANB, SNA, SNB angles and Ricketts’ NBa‐PtGn angle were measured to determine the skeletal malocclusion and the vertical pattern. Nine SNPs in BMP2, BMP4, SMAD6, RUNX2, WNT3A and WNT11 were genotyped. Chi‐squared test was used to compare genotypes among the groups. Multifactor dimensionality reduction (MDR) and binary logistic regression analysis, both using gender and age as co‐variables, were also used. We performed Bonferroni correction for multiple testing. Results Significant associations at P < .05 were observed for SNPs rs1005464 (P = .042) and rs235768 (P = .021) in BMP2 with mandibular retrognathism and for rs59983488 (RUNX2) with maxillary protrusion (P = .04) as well as for rs708111 (WNT3A) with skeletal class III (P = .02; dominant model), rs1533767 (WNT11) with a brachyfacial skeletal pattern (P = .01, OR = 0.10; dominant model) and for rs3934908 (SMAD6) with prognathism (P = .02; recessive model). After the Bonferroni correction, none of the SNPs remained associated. The MDR predicted some interaction for skeletal class II, dolichofacial and brachyfacial phenotypes. Conclusion Our results suggest that SNPs in BMP2, BMP4, SMAD6, RUNX2, WNT3A and WNT11 could be involved in the aetiology of sagittal and vertical malocclusions.

The purpose of the study was to investigate the association between single nucleotide polymorphisms (SNPs) in genes encoding estrogen receptors (ESR1 and ESR2, respectively) and delayed tooth emergence (DTE). This cross-sectional study was composed of biological unrelated children of both sexes, age ranging from 11 to 13 years old. DTE was defined when the successor primary tooth was still present in the oral cavity after its exfoliation time or the absence of the permanent tooth emergence into the oral cavity. Children were diagnosed with DTE when they had at least one delayed permanent tooth, according to age of exfoliation of each tooth proposed by The American Dental Association. Genomic DNA from saliva was used to evaluate the SNPs in ESR1 (rs9340799 and rs2234693) and ESR2 (rs1256049 and rs4986938) using Real-Time PCR. Chi-square or Fisher exact tests and Logistic Regression adjusted by age and gender were performed. SNP-SNP interaction was accessed by multifactor dimensionality reduction (MDR) analysis also adjusted by gender and age. The established alpha of this study was 5%. Among 537 included children, 296 (55%) were in the “DTE” group and the 241 (45%) were in the “Control” group. Age and gender were not statistically different among the groups (p>0.05). Genotype distribution of the SNPs rs9340799, rs2234693, rs1256049 and rs4986938 were not associated with DTE (p> 0.05). The models elected by MDR were not statistically significant either. Conclusions: The studied SNPs in ESR1 and ESR2 were not associated with permanent DTE. RESUMO O objetivo do presente estudo foi investigar a associação entre polimorfismos de nucleotídeo único (SNPs) em genes que codificam receptores de estrógeno (ESR1 e ESR2, respectivamente) e o retardo na emergência dentária (DTE). Este estudo transversal foi composto por crianças biológicas não relacionadas de ambos os sexos, com idades entre 11 e 13 anos. O DTE foi definido pela presença do dente decíduo na cavidade bucal após seu tempo e também, quando as crianças apresentaram pelo menos um dente permanente com atraso. O DNA genômico foi usado para avaliar os SNPs em ESR1 (rs9340799 e rs2234693) e ESR2 (rs1256049 e rs4986938) usando PCR em tempo real. Foram realizados testes Qui-quadrado ou exato de Fisher e Regressão Logística ajustados por idade e sexo. A interação SNP-SNP foi acessada pela análise de redução de dimensionalidade multifatorial (MDR), também ajustada por sexo e idade. O alfa de 5% foi estabelecido. Entre 537 crianças incluídas, 296 (55%) estavam no grupo “DTE” e 241 (45%) estavam no grupo “Controle”. A idade e o sexo não foram estatisticamente diferentes entre os grupos (p> 0,05). A distribuição de genótipos dos SNPs rs9340799, rs2234693, rs1256049 e rs4986938 não foi associada ao DTE (p> 0,05). Os modelos eleitos pelo MDR também não foram estatisticamente significativos. Conclusões: Os SNPs estudados na ESR1 e ESR2 não foram associados ao DTE na dentição permanente.

Ankylosed teeth may have a significant esthetic and functional impact especially at the anterior segment of the upper arch. Treatment of ankylosed teeth is challenging. The objective of this case report is to describe a clinical case in which an ankylosed tooth was treated with the use of osteogenic distraction associated with simplified orthodontic biomechanics. A 17-year-old female Caucasian patient presented with a Class II malocclusion, severe maxillary dental crowding, moderate mandibular dental crowding, anterior open bite, upper midline deviation to the right, and upper right central incisor in infraocclusion due to ankylosis. Treatment involved the use of the ankylosed tooth as anchorage for the distalization of the right upper segment to correct the Class II malocclusion and to create space prior to surgery. After one week of surgical osteotomy, traction of the tooth and bone segment was initiated with the use of intermaxillary elastics. The ankylosed tooth was moved to the desired position. Bone formation and mucogingival tissue adaptation were observed. Thus, esthetic and functional improvement was achieved. Osteogenic distraction associated with simplified orthodontic biomechanics is an alternative to the treatment of ankylosed teeth which can replace the use of distractor screws, making treatment simpler and more accessible.

In recent years, the use of radar wind profilers (RWP) at airports has grown significantly with the aim of supporting decision makers to maintain the safety of aircraft landings and takeoffs.The RWP provide vertical profiles of averaged horizontal wind speed and direction and vertical wind velocity for the entire Atmospheric Boundary Layer (ABL) and beyond. In addition, RWP with Radio-Acoustic Sounding System (RASS) are able to retrieve virtual temperature profiles in the ABL.RWP data evaluation is usually based on the so-called Doppler Beam Swinging method (DBS) which assumes homogeneity and stationarity of the wind field. Often, transient eddies violate this homogeneity and stationarity requirement. Hence, incorrect wind profiles can relate to transient eddies and present a problem for the forecast of high-impact weather phenomena in airports. This work intends to provide a method for removing outliers in such profiles based on historical data and other variables related to the Atmospheric Boundary Layer stability profile in the study region.For this study, a dataset of almost one year retrieved from a RWP LAP3000 with RASS Extension is used for a wind profile correction algorithm development.The algorithm consists of the detection of outliers in the wind profiles based on the thermodynamic structure of the ABL and the generation of the corrected profiles.Results show that the algorithm is capable of identifying and correcting unrealistic variations in speed caused by transient eddies. The method can be applied as a complement to the RWP data processing for better data reliability. Keywords: atmospheric boundary layer; stability profile; wind profile

Objective: The purpose of this study was to evaluate the correlation between the growth maturity indicators in orthodontic patients. Design: This cross-sectional study was performed on 37 orthodontic patients (17 males and 20 females). An anamnesis, clinical and image examination, and blood sample collection were performed. The inclusion criteria were non-syndromic Class II patients of both gender, age ranging between 10 to 16 years. The lateral cephalometric radiographs were evaluated using 6-stage cervical vertebrae maturation (CVM) technique. The hand-wrist radiographs were staged using the 11-stage skeletal maturation indicator (SMI) technique. Blood was collected in the same week of the images to quantify IGF-1 levels in serum. Data were tested for normality by Shapiro–Wilk test. The Pearson test was used to determine the correlation strength between the variables (alpha of 5%). Results: A strong correlation was observed only between SMI stages and CVM stages in the total sample (r=0.864; p