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35 results on '"Juli Vincent"'

1. Crystal structure of a phosphoribosylaminoimidazole mutase PurE (TM0446) from Thermotoga maritima at 1.77-A resolution

Author

Jeff Velasquez, Tanya Biorac, Adam Godzik, Peter Kuhn, Raymond C. Stevens, Guenter Wolf, Jie Ouyang, Heath E. Klock, Ross Floyd, Xiaoping Dai, Andreas Kreusch, Rebecca Page, Bill West, Keith O. Hodgson, Alyssa Robb, Hsiu-Ju Chiu, Jaume M. Canaves, Xianhong Wang, Scott A. Lesley, Linda S. Brinen, Andrew T. Morse, Juli Vincent, John S. Kovarik, Timothy M. McPhillips, Kin Moy, Marc-André Elsliger, Ashley M. Deacon, Mitchell D. Miller, Robert Schwarzenbacher, Ian A. Wilson, Frank von Delft, Eileen Ambing, Glen Spraggon, Mike DiDonato, Said Eshagi, Henry van den Bedem, Eric Hampton, Jamison Cambell, Eric Koesema, Carina Grittini, Kevin Quijano, Cathy Karlak, Polat Abdubek, Daniel McMullan, Inna Levin, John Wooley, Slawomir K. Grzechnik, Lukasz Jaroszewski, and Qingping Xu

Subjects
Models, Molecular, biology, Protein Conformation, Chemistry, Molecular Sequence Data, Resolution (electron density), Imidazoles, Reproducibility of Results, Crystal structure, Crystallography, X-Ray, biology.organism_classification, Lyase, Biochemistry, Crystallography, Mutase, Structural Biology, Thermotoga maritima, Amino Acid Sequence, Crystallization, Intramolecular Transferases, Molecular Biology
Published
2016

2. Crystal structure of a methionine aminopeptidase (TM1478) from Thermotoga maritima at 1.9 A resolution

Author

Peter Kuhn, Ross Floyd, Linda S. Brinen, Jaume M. Canaves, Guenter Wolf, Fred Rezezadeh, John Wooley, Kevin Quijano, Mitchell D. Miller, Xiaoping Dai, Raymond C. Stevens, Xianhong Wang, Scott A. Lesley, Marc-André Elsliger, Andreas Kreusch, Rebecca Page, Cathy Karlak, Kin Moy, Slawomir K. Grzechnik, Carina Grittini, Jeff Velasquez, Bill West, Adam Godzik, Qingping Xu, Said Eshagi, Frank von Delft, Ian A. Wilson, Alyssa Robb, Andrew T. Morse, Glen Spraggon, Juli Vincent, John S. Kovarik, Jie Ouyang, Lukasz Jaroszewski, Daniel McMullan, Robert Schwarzenbacher, Heath E. Klock, Eric Koesema, Ashley M. Deacon, Keith O. Hodgson, Henry van den Bedem, Timothy M. McPhillips, and Eric Sims

Subjects
Models, Molecular, Binding Sites, biology, Protein Conformation, Chemistry, Stereochemistry, Methionine aminopeptidase, Molecular Sequence Data, Resolution (electron density), Crystal structure, Crystallography, X-Ray, biology.organism_classification, Aminopeptidases, Biochemistry, Protein Structure, Tertiary, Bacterial Proteins, Structural Biology, Thermotoga maritima, Hydrolase, Methionyl Aminopeptidases, Amino Acid Sequence, Molecular Biology
Published
2016

3. Crystal structure of a putative PII-like signaling protein (TM0021) from Thermotoga maritima at 2.5 A resolution

Author

Jeff Velasquez, Mike DiDonato, Kevin Quijano, Xianhong Wang, Scott A. Lesley, Henry van den Bedem, Qingping Xu, Marc-André Elsliger, Polat Abdubek, Eric Hampton, Jie Ouyang, Timothy M. McPhillips, Xiaoping Dai, Jaume M. Canaves, Said Eshagi, Bill West, Guenter Wolf, Frank von Delft, Carina Grittini, Alyssa Robb, Lukasz Jaroszewski, Keith O. Hodgson, John Wooley, Andrew T. Morse, Mitchell D. Miller, Daniel McMullan, Inna Levin, Juli Vincent, H.-J. Chiu, Cathy Karlak, Robert Schwarzenbacher, Kin Moy, Slawomir K. Grzechnik, John S. Kovarik, Jamison Cambell, Eric Koesema, Peter Kuhn, Adam Godzik, Raymond C. Stevens, Tanja Biorac, Ross Floyd, Heath E. Klock, Linda S. Brinen, Andreas Kreusch, Rebecca Page, Glen Spraggon, Ashley M. Deacon, Ian A. Wilson, and Eileen Ambing

Subjects
Physics, Models, Molecular, biology, PII Nitrogen Regulatory Proteins, Resolution (electron density), Molecular Sequence Data, Synchrotron radiation, Reproducibility of Results, Particle accelerator, Crystal structure, biology.organism_classification, Crystallography, X-Ray, Biochemistry, Protein Structure, Secondary, law.invention, Crystallography, Bacterial Proteins, Structural Biology, law, Signaling proteins, Thermotoga maritima, Amino Acid Sequence, Molecular Biology, Signal Transduction
Published
2016

4. Crystal structure of a novel Thermotoga maritima enzyme (TM1112) from the cupin family at 1.83 A resolution

Author

Lukasz Jaroszewski, Eric Hampton, Carina Grittini, Keith O. Hodgson, Tanya Biorac, Said Eshaghi, Adam Godzik, Kin Moy, Kevin Quijano, Marc-André Elsliger, Daniel McMullan, Inna Levin, Fred Rezezadeh, John Wooley, Alyssa Robb, Raymond C. Stevens, Frank von Delft, Guenter Wolf, Heath E. Klock, Timothy M. McPhillips, Qingping Xu, Andrew T. Morse, Juli Vincent, Linda S. Brinen, Xiaoping Dai, Cathy Karlak, Eric Sims, Slawomir K. Grzechnik, Mike DiDonato, Jaume M. Canaves, Polat Abdubek, Henry van den Bedem, Peter Kuhn, Jie Ouyang, Bill West, Xianhong Wang, Scott A. Lesley, Ross Floyd, Jeff Velasquez, Eric Koesema, Ron Reyes, Robert Schwarzenbacher, Mitchell D. Miller, Andreas Kreusch, Rebecca Page, Ashley M. Deacon, Ian A. Wilson, Eileen Ambing, and Glen Spraggon

Subjects
chemistry.chemical_classification, Models, Molecular, Binding Sites, Magnetic Resonance Spectroscopy, biology, Resolution (electron density), Molecular Sequence Data, Crystal structure, biology.organism_classification, Biochemistry, Protein Structure, Secondary, Crystallography, Enzyme, chemistry, Bacterial Proteins, Structural Biology, Structural Homology, Protein, Thermotoga maritima, Amino Acid Sequence, Crystallization, Molecular Biology, Conserved Sequence
Published
2016

5. Crystal structure of gamma-glutamyl phosphate reductase (TM0293) from Thermotoga maritima at 2.0 A resolution

Author

Slawomir K. Grzechnik, Marc-André Elsliger, Raymond C. Stevens, John Wooley, Frank von Delft, Peter Kuhn, Jaume M. Canaves, Ross Floyd, Timothy M. McPhillips, Jeff Velasquez, Kin Moy, Lukasz Jaroszewski, Jie Ouyang, Eric Koesema, Ian A. Wilson, Robert Schwarzenbacher, Daniel McMullan, Michael S. Nelson, Alyssa Robb, Guenter Wolf, Rebecca Page, Juli Vincent, Andrew C. Morse, Bill West, Xiaoping Dai, Kevin Rodrigues, Carina Grittini, Linda S. Brinen, H. Klock, Ashley M. Deacon, Glen Spraggon, John S. Kovarik, Adam Godzik, Henry van den Bedem, Xianhong Wang, Scott A. Lesley, Andreas Kreusch, Keith O. Hodgson, and Mitchell D. Miller

Subjects
chemistry.chemical_classification, Models, Molecular, biology, Stereochemistry, Glutamate-5-Semialdehyde Dehydrogenase, Resolution (electron density), Molecular Sequence Data, Crystal structure, Reductase, biology.organism_classification, Crystallography, X-Ray, Biochemistry, Aldehyde Oxidoreductases, chemistry, Bacterial Proteins, Structural Biology, Oxidoreductase, Thermotoga maritima, Gamma-glutamyl phosphate, Amino Acid Sequence, Molecular Biology
Published
2016

6. Crystal structure of an Udp-n-acetylmuramate-alanine ligase MurC (TM0231) from Thermotoga maritima at 2.3 A resolution

Author

Guenter Wolf, Peter Kuhn, Daniel McMullan, Inna Levin, Ross Floyd, Hsiu-Ju Chiu, Carina Grittini, John S. Kovarik, Ian A. Wilson, Eric Hampton, Kin Moy, Eileen Ambing, Glen Spraggon, Alyssa Robb, Jaume M. Canaves, Heath E. Klock, Kevin Quijano, John Wooley, Marc-André Elsliger, Andrew T. Morse, Timothy M. McPhillips, Bill West, Juli Vincent, Christian C. Lee, Polat Abdubek, Jie Ouyang, Frank von Delft, Xiaoping Dai, Jeff Velasquez, Linda S. Brinen, Slawomir K. Grzechnik, Tanya Biorac, Adam Godzik, Raymond C. Stevens, Jamison Cambell, Eric Koesema, Ashley M. Deacon, Andreas Kreusch, Rebecca Page, Xianhong Wang, Scott A. Lesley, Robert Schwarzenbacher, Mike DiDonato, Cathy Karlak, Henry van den Bedem, Qingping Xu, Keith O. Hodgson, Lukasz Jaroszewski, Said Eshagi, and Mitchell D. Miller

Subjects
Models, Molecular, chemistry.chemical_classification, DNA ligase, biology, Chemistry, Molecular Sequence Data, Resolution (electron density), Crystal structure, Crystallography, X-Ray, biology.organism_classification, Biochemistry, Crystallography, Bacterial Proteins, UDP-N-acetylmuramate-alanine ligase, Structural Biology, Thermotoga maritima, Amino Acid Sequence, Peptide Synthases, Molecular Biology
Published
2016

7. Crystal structure of Hsp33 chaperone (TM1394) from Thermotoga maritima at 2.20 Å resolution

Author

Slawomir K. Grzechnik, Gye Won Han, Michael DiDonato, Heath E. Klock, Sanjay Agarwalla, John Wooley, Robert Schwarzenbacher, Daniel McMullan, Hsiu-Ju Chiu, Guenter Wolf, Ron Reyes, Marc-André Elsliger, Lukasz Jaroszewski, Polat Abdubek, Edward Nigoghossian, Raymond C. Stevens, Eric Hampton, Ashley M. Deacon, Tanya Biorac, Adam Godzik, Jaume M. Canaves, Qingping Xu, Ian A. Wilson, Jeff Velasquez, Mitchell D. Miller, Justin Haugen, Aprilfawn White, Eric Koesema, Kin Moy, Jessica Paulsen, Peter Kuhn, Eileen Ambing, Glen Spraggon, Juli Vincent, Kevin Quijano, Joanna Hale, Carina Grittini, Christopher L. Rife, Andreas Kreusch, Scott A. Lesley, Michael Hornsby, Henry van den Bedem, Herbert L. Axelrod, and Keith O. Hodgson

Subjects
biology, Chemistry, Molecular Sequence Data, Zinc Fingers, Crystal structure, biology.organism_classification, Biochemistry, Crystallography, Bacterial Proteins, Structural Biology, Thermotoga maritima, Chaperone (protein), Hsp33, biology.protein, Amino Acid Sequence, Crystallization, Molecular Biology, Heat-Shock Proteins, Molecular Chaperones
Published
2005

8. High-throughput Protein Production for X-ray Crystallography and Use of Size Exclusion Chromatography to Validate or Refute Computational Biological Unit Predictions

Author

Justin Haugen, Heath E. Klock, Joanna Hale, Juli Vincent, Edward Nigoghossian, Polat Abdubek, Daniel McMullan, Scott A. Lesley, Jaume M. Canaves, Kevin Quijano, and Jessica Paulsen

Subjects
Proteomics, Computational model, Protein Conformation, Size-exclusion chromatography, Computational Biology, General Medicine, Computational biology, Biology, Crystallography, X-Ray, biology.organism_classification, Biochemistry, Structural genomics, Crystallography, Protein structure, Bacterial Proteins, Structural Biology, Thermotoga maritima, Protein purification, Proteome, Chromatography, Gel, Genetics, Protein quaternary structure
Abstract

The production of large numbers of highly purified proteins for X-ray crystallography is a significant bottleneck in structural genomics. At the Joint Center for Structural Genomics (JCSG; http://www.jcsg.org), specific automated protein expression, purification, and analytical methods are being utilized to study the proteome of Thermotoga maritima. Anion exchange and size exclusion chromatography (SEC), intended for the production of highly purified proteins, have been automated and the procedures are described here in detail. Analytical SEC has been included as a standard quality control test. A biological unit (BU) is the macromolecule that has been proven or is presumed to be functional. Correct assignment of BUs from protein structures can be difficult. BU predictions obtained via the Protein Quaternary Structure file server (PQS; http://pqs.ebi.ac.uk/) were compared to SEC data for 16 representative T. maritima proteins whose structures were solved at the JCSG, revealing an inconsistency in five cases. Herein, we report that SEC can be used to validate or disprove PQS-derived oligomeric models. A substantial amount of associated SEC and structural data should enable us to use certain PQS parameters to gauge the accuracy of these computational models and to generally improve their predictions.

Published
2005

9. Crystal structure of the global regulatory protein CsrA from Pseudomonas putida at 2.05 Å resolution reveals a new fold

Author

Keith O. Hodgson, Marc-André Elsliger, Edward Nigoghossian, Aprilfawn White, Lukasz Jaroszewski, Michael Hornsby, Henry van den Bedem, Guenter Wolf, Slawomir K. Grzechnik, Gye Won Han, Qingping Xu, Tanya Biorac, Adam Godzik, Kevin Quijano, Raymond C. Stevens, Joanna Hale, Hsiu-Ju Chiu, Herbert L. Axelrod, Heath E. Klock, Jessica Paulsen, Mitchell D. Miller, Scott A. Lesley, Carina Grittini, John Wooley, Christopher L. Rife, Robert Schwarzenbacher, Daniel McMullan, Michael DiDonato, Justin Haugen, Ron Reyes, Polat Abdubek, Jeff Velasquez, Eric Koesema, Juli Vincent, Eric Hampton, Peter Kuhn, Andreas Kreusch, Ian A. Wilson, Eileen Ambing, Kin Moy, Jaume M. Canaves, Ashley M. Deacon, Glen Spraggon, and Eric Sims

Subjects
Models, Molecular, Regulation of gene expression, Protein Folding, biology, Pseudomonas putida, Chemistry, Molecular Sequence Data, RNA-Binding Proteins, RNA-binding protein, Crystal structure, Crystallography, X-Ray, biology.organism_classification, Biochemistry, Microbiology, Bacterial Proteins, Structural Biology, Amino Acid Sequence, Dimerization, Molecular Biology
Published
2005

10. Crystal structure of S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA) from Thermotoga maritima at 2.0 Å resolution reveals a new fold

Author

Polat Abdubek, Slawomir K. Grzechnik, Scott A. Lesley, Raymond C. Stevens, Kevin Quijano, Michael Hornsby, Henry van den Bedem, Guenter Wolf, Marc-André Elsliger, Heath E. Klock, Edward Nigoghossian, Lukasz Jaroszewski, Keith O. Hodgson, Jie Ouyang, John Wooley, Justin Haugen, Jaume M. Canaves, Jessica Paulsen, Aprilfawn White, Daniel McMullan, Inna Levin, Eric Koesema, Peter Kuhn, Ashley M. Deacon, Mitchell D. Miller, Gye Won Han, Qingping Xu, Ian A. Wilson, Herbert L. Axelrod, Hsiu-Ju Chiu, Eileen Ambing, Glen Spraggon, Tanya Biorac, Adam Godzik, Ron Reyes, Joanna Hale, Juli Vincent, Carina Grittini, Irimpan I. Mathews, Kin Moy, Andreas Kreusch, Michael DiDonato, Robert Schwarzenbacher, Eric Hampton, and Jeff Velasquez

Subjects
Models, Molecular, Protein Folding, biology, Protein Conformation, Chemistry, Stereochemistry, Crystal structure, Isomerase, Crystallography, X-Ray, Ligands, biology.organism_classification, Biochemistry, Protein structure, Structural Biology, Thermotoga maritima, Transfer RNA, Solvents, Protein folding, Isomerases, Molecular Biology
Published
2005

11. Crystal structure of a novel manganese-containing cupin (TM1459) from Thermotoga maritima at 1.65 Å resolution

Author

Keith O. Hodgson, Raymond C. Stevens, Guenter Wolf, Kevin Quijano, Alyssa Robb, Andrew T. Morse, Juli Vincent, Heath E. Klock, Adam Godzik, Eric Koesema, Marc-André Elsliger, Andreas Kreusch, Rebecca Page, Frank von Delft, Timothy M. McPhillips, Inna Levin, Lukasz Jaroszewski, Jaume M. Canaves, Carina Grittini, Eric Sims, Xianhong Wang, Qingping Xu, Scott A. Lesley, Ashley M. Deacon, Linda S. Brinen, Jeff Velasquez, Ron Reyes, Slawomir K. Grzechnik, Ian A. Wilson, Kin Moy, Mike DiDonato, Jie Ouyang, Said Eshagi, Fred Rezezadeh, Henry van den Bedem, Glen Spraggon, John S. Kovarik, John Wooley, Xiaoping Dai, Daniel McMullan, Peter Kuhn, Bill West, Eric Hampton, Ross Floyd, Cathy Karlak, Mitchell D. Miller, and Robert Schwarzenbacher

Subjects
Models, Molecular, Molecular Sequence Data, chemistry.chemical_element, Crystal structure, Manganese, Biochemistry, Protein Structure, Secondary, Bacterial protein, Protein structure, Bacterial Proteins, Structural Biology, Thermotoga maritima, Amino Acid Sequence, Molecular Biology, Binding Sites, biology, Chemistry, Resolution (electron density), biology.organism_classification, Protein Structure, Tertiary, Structural homology, Crystallography, Structural Homology, Protein, Multiprotein Complexes, Crystallization, Oxidoreductases
Published
2004

12. Crystal structure of a glycerophosphodiester phosphodiesterase (GDPD) from Thermotoga maritima (TM1621) at 1.60 Å resolution

Author

Slawomir K. Grzechnik, Henry van den Bedem, Bill West, Kevin Quijano, Guenter Wolf, Jamison Cambell, Eric Koesema, Heath E. Klock, Timothy M. McPhillips, Daniel McMullan, Kin Moy, Jaume M. Canaves, Andreas Kreusch, Rebecca Page, Eric Sims, Glen Spraggon, John Wooley, Fred Rezezadeh, John S. Kovarik, Marc-André Elsliger, Keith O. Hodgson, Alyssa Robb, Cathy Karlak, Xiaoping Dai, Jie Ouyang, Raymond C. Stevens, Andrew T. Morse, Juli Vincent, Frank von Delft, Said Eshagi, Eugenio Santelli, Carina Grittini, Xianhong Wang, Scott A. Lesley, Jeff Velasquez, Lukasz Jaroszewski, Robert Schwarzenbacher, Mitchell D. Miller, Qingping Xu, Ian A. Wilson, Ashley M. Deacon, Tanya Biorac, Adam Godzik, Linda S. Brinen, Peter Kuhn, and Ross Floyd

Subjects
Models, Molecular, Glycerophosphodiester phosphodiesterase, biology, Phosphoric Diester Hydrolases, Stereochemistry, Chemistry, Resolution (electron density), Reproducibility of Results, Phosphodiesterase, Crystal structure, Crystallography, X-Ray, biology.organism_classification, Biochemistry, Protein Structure, Secondary, Protein Structure, Tertiary, Structural Biology, Thermotoga maritima, Hydrolase, Crystallization, Molecular Biology
Published
2004

13. Crystal structure of an HEPN domain protein (TM0613) from Thermotoga maritima at 1.75 Å resolution

Author

Adam Godzik, Jie Ouyang, Xiaoping Dai, Bill West, Timothy M. McPhillips, Daniel McMullan, John S. Kovarik, Mitchell D. Miller, Guenter Wolf, Andreas Kreusch, Keith O. Hodgson, Rebecca Page, Marc-André Elsliger, Kin Moy, Robert Schwarzenbacher, Frank von Delft, Henry van den Bedem, Linda S. Brinen, Heath E. Klock, Jeff Velasquez, Raymond C. Stevens, Lukasz Jaroszewski, Peter Kuhn, Jaume M. Canaves, Heidi Erlandsen, Ross Floyd, Eric Koesema, Alyssa Robb, Andrew T. Morse, Juli Vincent, Ashley M. Deacon, Glen Spraggon, Slawomir K. Grzechnik, Ian A. Wilson, Xianhong Wang, Scott A. Lesley, John Wooley, Carina Grittini, and Kevin Quijano

Subjects
Physics, biology, Protein domain, Resolution (electron density), Synchrotron radiation, Particle accelerator, Crystal structure, biology.organism_classification, Biochemistry, law.invention, Crystallography, Structural Biology, law, Chemical physics, Thermotoga maritima, Molecular Biology
Published
2004

14. Crystal structure of uronate isomerase (TM0064) fromThermotoga maritimaat 2.85 Å resolution

Author

Keith O. Hodgson, Andreas Kreusch, Ian A. Wilson, Jie Ouyang, Henry van den Bedem, Lukasz Jaroszewski, Glen Spraggon, Xianhong Wang, Scott A. Lesley, Jaume M. Canaves, Chittibabu Guda, Alyssa Robb, Marc A. Elsliger, Bill West, Thomas L. Selby, Said Eshaghi, Raymond C. Stevens, Andrew T. Morse, Juli Vincent, Linda S. Brinen, Peter Kuhn, John S. Kovarik, Robert Schwarzenbacher, Ross Floyd, Adam Godzik, Daniel McMullan, John Wooley, Eric Koesema, Mitchell D. Miller, Jeff Velasquez, Ashley M. Deacon, Carina Grittini, Mark A. Miller, Slawomir K. Grzechnik, Kevin Rodrigues, Heath E. Klock, Xiaoping Dai, Kin Moy, Timothy M. McPhillips, Cathy Karlak, and Guenter Wolf

Subjects
Physics, Crystallography, biology, Structural Biology, Thermotoga maritima, Resolution (electron density), Crystal structure, Isomerase, biology.organism_classification, Molecular Biology, Biochemistry
Published
2003

15. Structure Analysis of Peptide Deformylases from Streptococcus pneumoniae, Staphylococcus aureus, Thermotoga maritima and Pseudomonas aeruginosa: Snapshots of the Oxygen Sensitivity of Peptide Deformylase

Author

Glen Spraggon, Andreas Kreusch, Daniel McMullan, Kenneth Ng, Heath E. Klock, Tanya Shin, Juli Vincent, Ian Warner, Chris C. Lee, Scott A. Lesley, and Christer Ericson

Subjects
Models, Molecular, Staphylococcus aureus, Molecular Sequence Data, Static Electricity, Peptide, Biology, Crystallography, X-Ray, medicine.disease_cause, Aminopeptidases, Mass Spectrometry, Protein Structure, Secondary, Amidohydrolases, Microbiology, Peptide deformylase, Structural Biology, Hydrolase, medicine, Thermotoga maritima, Amino Acid Sequence, Molecular Biology, Escherichia coli, chemistry.chemical_classification, Binding Sites, Molecular Structure, Sequence Homology, Amino Acid, Pseudomonas aeruginosa, biology.organism_classification, Recombinant Proteins, Protein Structure, Tertiary, N-terminus, Streptococcus pneumoniae, Enzyme, Biochemistry, chemistry, Oxidation-Reduction
Abstract

Peptide deformylase (PDF) has received considerable attention during the last few years as a potential target for a new type of antibiotics. It is an essential enzyme in eubacteria for the removal of the formyl group from the N terminus of the nascent polypeptide chain. We have solved the X-ray structures of four members of this enzyme family, two from the Gram-positive pathogens Streptococcus pneumoniae and Staphylococcus aureus , and two from the Gram-negative bacteria Thermotoga maritima and Pseudomonas aeruginosa . Combined with the known structures from the Escherichia coli enzyme and the recently solved structure of the eukaryotic deformylase from Plasmodium falciparum , a complete picture of the peptide deformylase structure and function relationship is emerging. This understanding could help guide a more rational design of inhibitors. A structure-based comparison between PDFs reveals some conserved differences between type I and type II enzymes. Moreover, our structures provide insights into the known instability of PDF caused by oxidation of the metal-ligating cysteine residue.

Published
2003

16. Crystal structure of thy1, a thymidylate synthase complementing protein fromThermotoga maritimaat 2.25 Å resolution

Author

Susan S. Taylor, Xiaoping Dai, Peter Kuhn, H. Klock, Eric Koesema, Ross Floyd, Ian A. Wilson, Irimpan I. Mathews, Jie Ouyang, Mark A. Miller, Kin Moy, Guenter Wolf, Lukasz Jaroszewski, Slawomir K. Grzechnik, Andreas Kreusch, Cathy Karlak, John M. Kovarik, Jeff Velasquez, Raymond C. Stevens, Alyssa Robb, Xianhong Wang, Scott A. Lesley, Timothy M. McPhillips, Linda S. Brinen, Carina Grittini, Ashley M. Deacon, Glen Spraggon, Andrew T. Morse, Juli Vincent, Mitchell D. Miller, Said Eshaghi, John Wooley, Jaume M. Canaves, Chittibabu Guda, Bill West, Thomas L. Selby, Kevin Rodrigues, Daniel McMullan, Adam Godzik, Henry van den Bedem, Keith O. Hodgson, and Marc A. Elsliger

Subjects
biology, Biochemistry, Structural Biology, Thermotoga maritima, Resolution (electron density), biology.protein, Crystal structure, biology.organism_classification, Molecular Biology, Thymidylate synthase
Published
2002

17. Crystal structure of a conserved hypothetical protein (gi: 13879369) from Mouse at 1.90 A resolution reveals a new fold

Author

Ian A. Wilson, Eileen Ambing, Ashley M. Deacon, Andreas Kreusch, Jeff Velasquez, Jessica Paulsen, Juli Vincent, Hsiu-Ju Chiu, Herbert L. Axelrod, Polat Abdubek, Michael DiDonato, Gye Won Han, Carina Grittini, Jaume M. Canaves, Raymond C. Stevens, Guenter Wolf, Qingping Xu, Heath E. Klock, Slawomir K. Grzechnik, Michael Hornsby, Christopher L. Rife, Henry van den Bedem, Scott A. Lesley, Kevin Quijano, Marc-André Elsliger, Peter Kuhn, Edward Nigoghossian, Keith O. Hodgson, Aprilfawn White, Joanna Hale, Tanya Biorac, Adam Godzik, Eric Hampton, Mitchell D. Miller, Eric Koesema, Justin Haugen, Eric Sims, John Wooley, Ron Reyes, Daniel McMullan, Robert Schwarzenbacher, Kin Moy, Lukasz Jaroszewski, and Glen Spraggon

Subjects
Models, Molecular, Protein Folding, Chemistry, Hypothetical protein, Proteins, Fold (geology), Crystal structure, Crystallography, X-Ray, Biochemistry, Protein Structure, Secondary, Molecular Weight, Crystallography, Mice, Open Reading Frames, Structural Biology, Transferase, Animals, Databases, Protein, Molecular Biology, Conserved Sequence
Published
2005

18. Crystal structure of a putative modulator of DNA gyrase (pmbA) from Thermotoga maritima at 1.95 A resolution reveals a new fold

Author

Peter Kuhn, Kevin Quijano, Slawomir K. Grzechnik, Marc-André Elsliger, Ian A. Wilson, Justin Haugen, Polat Abdubek, Edward Nigoghossian, Aprilfawn White, Eileen Ambing, John Wooley, Glen Spraggon, Kin Moy, Carina Grittini, Robert Schwarzenbacher, Daniel McMullan, Heath E. Klock, Hsiu-Ju Chiu, Raymond C. Stevens, Ashley M. Deacon, Guenter Wolf, Mitchell D. Miller, Ron Reyes, Joanna Hale, Jessica Paulsen, Eric Koesema, Gye Won Han, Scott A. Lesley, Andreas Kreusch, Lukasz Jaroszewski, Tanya Biorac, Adam Godzik, Juli Vincent, Qingping Xu, Keith O. Hodgson, Michael Hornsby, Henry van den Bedem, Eric Sims, Herbert L. Axelrod, Michael DiDonato, Jeff Velasquez, Christopher L. Rife, Eric Hampton, and Jaume M. Canaves

Subjects
Regulation of gene expression, Models, Molecular, Protein Folding, biology, Chemistry, Stereochemistry, Molecular Sequence Data, Crystal structure, biology.organism_classification, Crystallography, X-Ray, Biochemistry, DNA gyrase, Bacterial Proteins, Structural Biology, DNA Gyrase, Thermotoga maritima, Amino Acid Sequence, Molecular Biology, Dimerization
Published
2005

19. Crystal structure of an Apo mRNA decapping enzyme (DcpS) from Mouse at 1.83 A resolution

Author

John Wooley, Justin Haugen, Peter Kuhn, Heath E. Klock, Scott A. Lesley, Joanna Hale, Hsiu-Ju Chiu, Guenter Wolf, Tanya Biorac, Adam Godzik, Qingping Xu, Xiaoping Dai, Polat Abdubek, Slawomir K. Grzechnik, Ron Reyes, Glen Spraggon, Michael DiDonato, Eric Koesema, Carina Grittini, Gye Won Han, Raymond C. Stevens, Marc-André Elsliger, Jeff Velasquez, Edward Nigoghossian, Daniel McMullan, Mitchell D. Miller, Kevin Quijano, Keith O. Hodgson, Aprilfawn White, Robert Schwarzenbacher, Eric Hampton, Juli Vincent, Kin Moy, Lukasz Jaroszewski, Ian A. Wilson, Herbert L. Axelrod, Michael Hornsby, Henry van den Bedem, Eileen Ambing, Ashley M. Deacon, Jessica Paulsen, Jaume M. Canaves, Andreas Kreusch, and Timothy M. McPhillips

Subjects
Models, Molecular, Messenger RNA, Chemistry, Resolution (electron density), DCPS, Molecular Sequence Data, RNA-binding protein, Crystal structure, Crystallography, X-Ray, Biochemistry, Sensitivity and Specificity, Protein Structure, Secondary, Mice, Apoenzymes, Structural Biology, Endoribonucleases, Animals, Amino Acid Sequence, RNA, Messenger, Molecular Biology, Decapping enzyme
Published
2005

20. Crystal structure of an indigoidine synthase A (IndA)-like protein (TM1464) from Thermotoga maritima at 1.90 A resolution reveals a new fold

Author

Jaume M. Canaves, Slawomir K. Grzechnik, Peter Kuhn, Heath E. Klock, Lukasz Jaroszewski, Jie Ouyang, Ron Reyes, Raymond C. Stevens, John Wooley, Keith O. Hodgson, Olga Zagnitko, Kin Moy, Polat Abdubek, Guenter Wolf, Andreas Kreusch, Rebecca Page, Jeff Velasquez, Glen Spraggon, Michael DiDonato, Joanna Hale, Xianhong Wang, Scott A. Lesley, Qingping Xu, Mitchell D. Miller, Cathy Karlak, Michael Hornsby, Henry van den Bedem, Daniel McMullan, Inna Levin, Alyssa Robb, Kevin Quijano, Bill West, Eric Sims, Eric Hampton, Andrew T. Morse, Juli Vincent, Jamison Cambell, Eric Koesema, Justin Haugen, Robert Schwarzenbacher, Ashley M. Deacon, Carina Grittini, Ian A. Wilson, Hsiu-Ju Chiu, Eileen Ambing, Marc-André Elsliger, Edward Nigoghossian, Tanya Biorac, Adam Godzik, and Gye Won Han

Subjects
Models, Molecular, Protein Folding, biology, ATP synthase, Chemistry, Stereochemistry, Protein Conformation, Fold (geology), Crystal structure, biology.organism_classification, Crystallography, X-Ray, Biochemistry, Bacterial Proteins, Structural Biology, Thermotoga maritima, biology.protein, Molecular Biology, Indigoidine, Piperidones
Published
2005

21. Crystal structure of an alanine-glyoxylate aminotransferase from Anabaena sp. at 1.70 A resolution reveals a noncovalently linked PLP cofactor

Author

Guenter Wolf, Gye Won Han, Kin Moy, Kevin Quijano, Slawomir K. Grzechnik, Michael Hornsby, Henry van den Bedem, Andreas Kreusch, Jessica Paulsen, Heath E. Klock, Polat Abdubek, Hsiu-Ju Chiu, Rebecca Page, Qingping Xu, Ron Reyes, Marc-André Elsliger, Peter Kuhn, Edward Nigoghossian, Eric Hampton, Andrew T. Morse, Jeff Velasquez, Frank von Delft, Juli Vincent, Lukasz Jaroszewski, Tanya Biorac, Adam Godzik, Olga Zagnitko, Michael DiDonato, Daniel McMullan, Inna Levin, Carina Grittini, Bill West, Keith O. Hodgson, Joanna Hale, Xiaoping Dai, Eric Koesema, Mitchell D. Miller, Justin Haugen, John Wooley, Xianhong Wang, Scott A. Lesley, Timothy M. McPhillips, Ashley M. Deacon, Glen Spraggon, Eric Sims, Robert Schwarzenbacher, Ian A. Wilson, Eileen Ambing, Jie Ouyang, Raymond C. Stevens, Jaume M. Canaves, and Aprilfawn White

Subjects
Models, Molecular, Protein Conformation, Stereochemistry, Molecular Sequence Data, Anabaena sp, Alanine glyoxylate aminotransferase, Electrons, Crystal structure, Crystallography, X-Ray, Biochemistry, Cofactor, Structural Biology, Humans, Transferase, Amino Acid Sequence, Molecular Biology, Transaminases, Sequence Homology, Amino Acid, biology, Chemistry, Resolution (electron density), Proteins, Stereoisomerism, Anabaena, Protein Structure, Tertiary, biology.protein
Published
2005

22. Crystal structure of an alpha/beta serine hydrolase (YDR428C) from Saccharomyces cerevisiae at 1.85 A resolution

Author

Timothy M. McPhillips, Eric Sims, Eric Hampton, Xiaoping Dai, Jaume M. Canaves, John Wooley, Aprilfawn White, Carina Grittini, Slawomir K. Grzechnik, Daniel McMullan, Inna Levin, Joanna Hale, Heath E. Klock, Lukasz Jaroszewski, Peter Kuhn, Keith O. Hodgson, Justin Haugen, Olga Zagnitko, Guenter Wolf, Kevin Quijano, Kin Moy, Hsiu-Ju Chiu, W. Peti, Joseph W. Arndt, Qingping Xu, Polat Abdubek, Mitchell D. Miller, Andrew T. Morse, Jeff Velasquez, Juli Vincent, Bill West, Jie Ouyang, Marc-André Elsliger, Gye Won Han, Edward Nigoghossian, Andreas Kreusch, Michael Hornsby, Henry van den Bedem, Rebecca Page, Robert Schwarzenbacher, Michael Didonato, Eric Koesema, Frank von Delft, Xianhong Wang, Scott A. Lesley, Ashley M. Deacon, Glen Spraggon, Raymond C. Stevens, Ian A. Wilson, Eileen Ambing, Ron Reyes, Tanya Biorac, and Adam Godzik

Subjects
Models, Molecular, Saccharomyces cerevisiae Proteins, Hydrolases, Protein Conformation, Molecular Sequence Data, Saccharomyces cerevisiae, Molecular Conformation, Alpha (ethology), Crystal structure, Crystallography, X-Ray, Biochemistry, Protein Structure, Secondary, Structural Biology, Hydrolase, Serine, Amino Acid Sequence, Beta (finance), Molecular Biology, Binding Sites, biology, Chemistry, Resolution (electron density), Computational Biology, Serine hydrolase, biology.organism_classification, Protein Structure, Tertiary, Software
Published
2005

23. Crystal structure of a formiminotetrahydrofolate cyclodeaminase (TM1560) from Thermotoga maritima at 2.80 A resolution reveals a new fold

Author

Keith O. Hodgson, Kin Moy, Jie Ouyang, Polat Abdubek, Guenter Wolf, Heath E. Klock, Hsiu-Ju Chiu, Xiaoping Dai, Mitchell D. Miller, Marc-André Elsliger, Frank von Delft, Robert Schwarzenbacher, Michael Hornsby, Henry van den Bedem, Carina Grittini, Jeff Velasquez, Tanya Biorac, Adam Godzik, Lukasz Jaroszewski, Olga Zagnitko, Eric Sims, Raymond C. Stevens, Qingping Xu, Eric Koesema, Andreas Kreusch, Michael DiDonato, Rebecca Page, Bill West, Jaume M. Canaves, Peter Kuhn, Aprilfawn White, Daniel McMullan, Inna Levin, Alyssa Robb, Andrew T. Morse, Juli Vincent, Eric Hampton, Ian A. Wilson, Slawomir K. Grzechnik, Xianhong Wang, Scott A. Lesley, Eileen Ambing, Ashley M. Deacon, Glen Spraggon, John Wooley, Kevin Quijano, and Ron Reyes

Subjects
Models, Molecular, Ammonia-Lyases, Stereochemistry, Protein Conformation, Molecular Sequence Data, Molecular Conformation, Crystal structure, Crystallography, X-Ray, Biochemistry, Protein Structure, Secondary, Formiminotetrahydrofolate cyclodeaminase, Structural Biology, Enzyme Stability, Thermotoga maritima, Amino Acid Sequence, Molecular Biology, Binding Sites, biology, Chemistry, Fold (geology), Lyase, biology.organism_classification, Protein Structure, Tertiary, Models, Chemical, Crystallization
Published
2005

24. Crystal structure of a tandem cystathionine-beta-synthase (CBS) domain protein (TM0935) from Thermotoga maritima at 1.87 A resolution

Author

Jaume M. Canaves, Timothy M. McPhillips, Kevin Quijano, Linda S. Brinen, Mitchell D. Miller, Peter Kuhn, Polat Abdubek, Ross Floyd, Mike DiDonato, John Wooley, Guenter Wolf, Jamison Cambell, Eric Koesema, Marc-André Elsliger, Raymond C. Stevens, Carina Grittini, Daniel McMullan, Inna Levin, Xiaoping Dai, Henry van den Bedem, Said Eshagi, Cathy Karlak, Alyssa Robb, Andrew T. Morse, Juli Vincent, Kin Moy, Jie Ouyang, Slawomir K. Grzechnik, Andreas Kreusch, Rebecca Page, Bill West, Frank von Delft, Keith O. Hodgson, Heath E. Klock, Hsiu-Ju Chiu, Robert Schwarzenbacher, Xianhong Wang, Scott A. Lesley, John S. Kovarik, Jeff Velasquez, Lukasz Jaroszewski, Qingping Xu, Eric Hampton, Tanya Biorac, Adam Godzik, Ashley M. Deacon, Ian A. Wilson, Eileen Ambing, and Glen Spraggon

Subjects
Models, Molecular, Crystallography, biology, Tandem, Resolution (electron density), Molecular Sequence Data, CBS domain, Cystathionine beta-Synthase, Crystal structure, biology.organism_classification, Biochemistry, Cystathionine beta synthase, Protein Structure, Secondary, Protein Structure, Tertiary, Bacterial Proteins, Structural Biology, Thermotoga maritima, biology.protein, Transferase, Amino Acid Sequence, Molecular Biology
Published
2004

25. Crystal structure of an allantoicase (YIR029W) from Saccharomyces cerevisiae at 2.4 A resolution

Author

Lukasz Jaroszewski, Alyssa Robb, Glen Spraggon, John Wooley, Andrew T. Morse, Jie Ouyang, Juli Vincent, Qingping Xu, Jaume M. Canaves, John S. Kovarik, Slawomir K. Grzechnik, Jamison Cambell, Eric Koesema, Mitchell D. Miller, Guenter Wolf, Linda S. Brinen, Daniel McMullan, Bill West, Xianhong Wang, Scott A. Lesley, Eric Hampton, Fred Rezezadeh, Raymond C. Stevens, Inna Levin, Timothy M. McPhillips, Heath E. Klock, Ian A. Wilson, Eric Sims, Mike DiDonato, Jeff Velasquez, Carina Grittini, Peter Kuhn, Eileen Ambing, Henry van den Bedem, Andreas Kreusch, Rebecca Page, Cathy Karlak, Ross Floyd, Robert Schwarzenbacher, Keith O. Hodgson, Ashley M. Deacon, Xiaoping Dai, Kevin Quijano, Kin Moy, Polat Abdubek, Ron Reyes, Tanya Biorac, Adam Godzik, Marc-André Elsliger, Frank von Delft, and Hsiu-Ju Chiu

Subjects
Models, Molecular, biology, Chemistry, Molecular Sequence Data, Resolution (electron density), Saccharomyces cerevisiae, Crystal structure, Crystallography, X-Ray, biology.organism_classification, Biochemistry, Protein Structure, Secondary, Ureohydrolases, Protein Structure, Tertiary, Multienzyme Complexes, Structural Homology, Protein, Structural Biology, Hydrolase, Allantoicase, Amino Acid Sequence, Molecular Biology, Conserved Sequence
Published
2004

26. Crystal structure of an orphan protein (TM0875) from Thermotoga maritima at 2.00-A resolution reveals a new fold

Author

Lukasz Jaroszewski, Ian A. Wilson, Alyssa Robb, Bill West, Carina Grittini, Glen Spraggon, Andrew T. Morse, Juli Vincent, Said Eshagi, Marc-André Elsliger, Timothy M. McPhillips, Raymond C. Stevens, Kevin Quijano, Keith O. Hodgson, Ashley M. Deacon, Frank von Delft, Guenter Wolf, Jaume M. Canaves, Mitchell D. Miller, Kin Moy, Adam Godzik, Xianhong Wang, Scott A. Lesley, Jie Ouyang, Eric Koesema, Henry van den Bedem, Linda S. Brinen, Robert Schwarzenbacher, Xiaoping Dai, Constantina Bakolitsa, Daniel McMullan, Andreas Kreusch, Rebecca Page, Peter Kuhn, H. Klock, Ross Floyd, Cathy Karlak, Slawomir K. Grzechnik, John Wooley, Jeff Velasquez, and John S. Kovarik

Subjects
Models, Molecular, Molecular Sequence Data, Sequence Homology, Fold (geology), Crystal structure, Biology, biology.organism_classification, Crystallography, X-Ray, Biochemistry, Protein Structure, Secondary, Crystallography, Bacterial Proteins, Structural Biology, Structural Homology, Protein, Thermotoga maritima, Multiprotein Complexes, Amino Acid Sequence, Molecular Biology
Published
2004

27. Crystal structure of a putative NADPH-dependent oxidoreductase (GI: 18204011) from mouse at 2.10 A resolution

Author

Andreas Kreusch, Rebecca Page, Qingping Xu, Henry van den Bedem, Carina Grittini, Xiaoping Dai, Raymond C. Stevens, Slawomir K. Grzechnik, Hsiu-Ju Chiu, Jie Ouyang, Guenter Wolf, Bill West, Kevin Quijano, Heath E. Klock, Xianhong Wang, Scott A. Lesley, Michael Didonato, Jamison Cambell, Polat Abdubek, Marc-André Elsliger, Jaume M. Canaves, Eric Koesema, Kin Moy, Lukasz Jaroszewski, Frank von Delft, Alyssa Robb, Keith O. Hodgson, Andrew T. Morse, Juli Vincent, Inna Levin, Jeff Velasquez, John Wooley, Cathy Karlak, Mitchell D. Miller, Glen Spraggon, Peter Kuhn, Eric Hampton, Robert Schwarzenbacher, Tanya Biorac, Adam Godzik, Daniel McMullan, Ron Reyes, Timothy M. McPhillips, Eric Sims, Ian A. Wilson, Eileen Ambing, and Ashley M. Deacon

Subjects
chemistry.chemical_classification, Models, Molecular, Binding Sites, Chemistry, Stereochemistry, Resolution (electron density), Molecular Sequence Data, Crystal structure, Crystallography, X-Ray, Biochemistry, Protein Structure, Secondary, Protein Structure, Tertiary, Crystallography, Mice, Structural Biology, Oxidoreductase, Structural Homology, Protein, Animals, Amino Acid Sequence, Quinone Reductases, Oxidoreductases, Molecular Biology, NADP
Published
2004

28. Crystal structure of a ribose-5-phosphate isomerase RpiB (TM1080) from Thermotoga maritima at 1.90 A resolution

Author

Xianhong Wang, Scott A. Lesley, Jie Ouyang, Timothy M. McPhillips, Marc-André Elsliger, Kevin Quijano, John S. Kovarik, Linda S. Brinen, Guenter Wolf, Ashley M. Deacon, Robert Schwarzenbacher, Kin Moy, Henry van den Bedem, Frank von Delft, John Wooley, Raymond C. Stevens, Glen Spraggon, Peter Kuhn, Alyssa Robb, Jeff Velasquez, Ross Floyd, Andrew T. Morse, Mitchell D. Miller, Juli Vincent, Jaume M. Canaves, Andreas Kreusch, Keith O. Hodgson, Rebecca Page, Xiaoping Dai, Slawomir K. Grzechnik, Bill West, Said Eshagi, Cathy Karlak, Ian A. Wilson, Carina Grittini, Eric Koesema, Adam Godzik, Daniel McMullan, Inna Levin, Lukasz Jaroszewski, Qingping Xu, and Heath E. Klock

Subjects
Models, Molecular, biology, Chemistry, Stereochemistry, Resolution (electron density), Reproducibility of Results, Isomerase, Crystal structure, biology.organism_classification, Crystallography, X-Ray, Biochemistry, Protein Structure, Secondary, Ribose-5-phosphate isomerase, Structural Biology, Thermotoga maritima, Crystallization, Molecular Biology, Aldose-Ketose Isomerases
Published
2004

29. Crystal structure of a PIN (PilT N-terminus) domain (AF0591) from Archaeoglobus fulgidus at 1.90 A resolution

Author

Jie Ouyang, Andreas Kreusch, Rebecca Page, John Wooley, Heath E. Klock, Ashley M. Deacon, Mitchell D. Miller, Hsiu-Ju Chiu, Jaume M. Canaves, Glen Spraggon, Mike DiDonato, Jamison Campbell, Henry van den Bedem, Linda S. Brinen, Guenter Wolf, Kin Moy, Eric Hampton, Marc-André Elsliger, Keith O. Hodgson, Tanya Biorac, Adam Godzik, Ian A. Wilson, Peter Kuhn, Jeff Velasquez, Fred Rezezadeh, Ross Floyd, Bill West, Eileen Ambing, Ron Reyes, Raymond C. Stevens, Timothy M. McPhillips, Frank von Delft, Xiaoping Dai, Eric Sims, John S. Kovarik, Eric Koesema, Robert Schwarzenbacher, Alyssa Robb, Andrew T. Morse, Juli Vincent, Xianhong Wang, Scott A. Lesley, Kevin Quijano, Carina Grittini, Slawomir K. Grzechnik, Polat Abdubek, Daniel McMullan, Inna Levin, Cathy Karlak, Qingping Xu, and Lukasz Jaroszewski

Subjects
Exonucleases, Models, Molecular, Protein Folding, Protein Conformation, Archaeal Proteins, Molecular Sequence Data, Crystal structure, Siphoviridae, Crystallography, X-Ray, Biochemistry, Protein Structure, Secondary, Viral Proteins, Protein structure, Structural Biology, Amino Acid Sequence, Molecular Biology, Peptide sequence, Binding Sites, Sequence Homology, Amino Acid, Resolution (electron density), Archaeoglobus fulgidus, Protein Structure, Tertiary, N-terminus, Crystallography, Protein folding, PIN domain
Published
2004

30. Crystal structure of an aspartate aminotransferase (TM1255) from Thermotoga maritima at 1.90 A resolution

Author

Glen Spraggon, Slawomir K. Grzechnik, Andreas Kreusch, Rebecca Page, Kevin Quijano, Polat Abdubek, Jie Ouyang, Marc-André Elsliger, H.-J. Chiu, Cathy Karlak, Adam Godzik, Raymond C. Stevens, Alyssa Robb, Said Eshagi, Frank von Delft, Kin Moy, Tanja Biorac, Guenter Wolf, Andrew T. Morse, Juli Vincent, Linda S. Brinen, Heath E. Klock, Jaume M. Canaves, Carina Grittini, Robert Schwarzenbacher, John Wooley, Ashley M. Deacon, Daniel McMullan, Inna Levin, Peter Kuhn, Xiaoping Dai, Jamison Cambell, Ian A. Wilson, Ross Floyd, Eric Koesema, Mitchell D. Miller, Jeff Velasquez, Eileen Ambing, Xianhong Wang, Scott A. Lesley, Keith O. Hodgson, John S. Kovarik, Lukasz Jaroszewski, Mike DiDonato, Henry van den Bedem, Qingping Xu, Eric Hampton, Bill West, and Timothy M. McPhillips

Subjects
Models, Molecular, biology, Chemistry, Resolution (electron density), Molecular Sequence Data, Crystal structure, biology.organism_classification, Crystallography, X-Ray, Biochemistry, Crystallography, Bacterial Proteins, Structural Biology, Thermotoga maritima, Transferase, Amino Acid Sequence, Aspartate Aminotransferases, Molecular Biology, Peptide sequence
Published
2004

31. Crystal structure of an HEPN domain protein (TM0613) from Thermotoga maritima at 1.75 A resolution

Author

Heidi, Erlandsen, Jaume M, Canaves, Marc-André, Elsliger, Frank, von Delft, Linda S, Brinen, Xiaoping, Dai, Ashley M, Deacon, Ross, Floyd, Adam, Godzik, Carina, Grittini, Slawomir K, Grzechnik, Lukasz, Jaroszewski, Heath E, Klock, Eric, Koesema, John S, Kovarik, Andreas, Kreusch, Peter, Kuhn, Scott A, Lesley, Daniel, McMullan, Timothy M, McPhillips, Mitchell D, Miller, Andrew, Morse, Kin, Moy, Jie, Ouyang, Rebecca, Page, Alyssa, Robb, Kevin, Quijano, Robert, Schwarzenbacher, Glen, Spraggon, Raymond C, Stevens, Henry, van den Bedem, Jeff, Velasquez, Juli, Vincent, Xianhong, Wang, Bill, West, Guenter, Wolf, Keith O, Hodgson, John, Wooley, and Ian A, Wilson

Subjects
Models, Molecular, Bacterial Proteins, Genes, Bacterial, Molecular Sequence Data, Reproducibility of Results, Thermotoga maritima, Amino Acid Sequence, Crystallography, X-Ray, Protein Structure, Tertiary
Published
2004

32. Crystal structure of an iron-containing 1,3-propanediol dehydrogenase (TM0920) from Thermotoga maritima at 1.3 A resolution

Author

John Wooley, Xiaoping Dai, Glen Spraggon, Said Eshaghi, Keith O. Hodgson, Slawomir K. Grzechnik, Jaume M. Canaves, Mitchell D. Miller, Lukasz Jaroszewski, John S. Kovarik, Jie Ouyang, Eric Koesema, Timothy M. McPhillips, Adam Godzik, Frank von Delft, Kin Moy, Alyssa Robb, Andrew T. Morse, Juli Vincent, Jeff Velasquez, Raymond C. Stevens, Guenter Wolf, Ian A. Wilson, Andreas Kreusch, Peter Kuhn, Rebecca Page, Ross Floyd, Robert Schwarzenbacher, Marc A. Elsliger, Xianhong Wang, Scott A. Lesley, Ashley M. Deacon, Linda S. Brinen, Henry van den Bedem, Kevin Rodrigues, Bill West, Thomas L. Selby, Daniel McMullan, Carina Grittini, Mark A. Miller, Cathy Karlak, Chittibabu Guda, and Heath E. Klock

Subjects
Models, Molecular, Stereochemistry, Molecular Sequence Data, Alcohol oxidoreductase, Dehydrogenase, Crystal structure, Crystallography, X-Ray, Biochemistry, chemistry.chemical_compound, Bacterial Proteins, Structural Biology, Oxidoreductase, Iron-Binding Proteins, Thermotoga maritima, 1,3-Propanediol, Amino Acid Sequence, Molecular Biology, Alcohol dehydrogenase, chemistry.chemical_classification, biology, Resolution (electron density), Alcohol Dehydrogenase, biology.organism_classification, Alcohol Oxidoreductases, chemistry, biology.protein
Published
2004

33. Crystal structure of uronate isomerase (TM0064) from Thermotoga maritima at 2.85 A resolution

Author

Robert, Schwarzenbacher, Jaume M, Canaves, Linda S, Brinen, Xiaoping, Dai, Ashley M, Deacon, Marc A, Elsliger, Said, Eshaghi, Ross, Floyd, Adam, Godzik, Carina, Grittini, Slawomir K, Grzechnik, Chittibabu, Guda, Lukasz, Jaroszewski, Cathy, Karlak, Heath E, Klock, Eric, Koesema, John S, Kovarik, Andreas, Kreusch, Peter, Kuhn, Scott A, Lesley, Daniel, McMullan, Timothy M, McPhillips, Mark A, Miller, Mitchell D, Miller, Andrew, Morse, Kin, Moy, Jie, Ouyang, Alyssa, Robb, Kevin, Rodrigues, Thomas L, Selby, Glen, Spraggon, Raymond C, Stevens, Henry, van den Bedem, Jeff, Velasquez, Juli, Vincent, Xianhong, Wang, Bill, West, Guenter, Wolf, Keith O, Hodgson, John, Wooley, and Ian A, Wilson

Subjects
Models, Molecular, Molecular Structure, Thermotoga maritima, Crystallography, X-Ray, Aldose-Ketose Isomerases, Protein Structure, Tertiary
Published
2003

34. Crystal structure of thy1, a thymidylate synthase complementing protein from Thermotoga maritima at 2.25 A resolution

Author

Peter, Kuhn, Scott A, Lesley, Irimpan I, Mathews, Jaume M, Canaves, Linda S, Brinen, Xiaoping, Dai, Ashley M, Deacon, Marc A, Elsliger, Said, Eshaghi, Ross, Floyd, Adam, Godzik, Carina, Grittini, Slawomir K, Grzechnik, Chittibabu, Guda, Keith O, Hodgson, Lukasz, Jaroszewski, Cathy, Karlak, Heath E, Klock, Eric, Koesema, John M, Kovarik, Andreas T, Kreusch, Daniel, McMullan, Timothy M, McPhillips, Mark A, Miller, Mitchell, Miller, Andrew, Morse, Kin, Moy, Jie, Ouyang, Alyssa, Robb, Kevin, Rodrigues, Thomas L, Selby, Glen, Spraggon, Raymond C, Stevens, Susan S, Taylor, Henry, van den Bedem, Jeff, Velasquez, Juli, Vincent, Xianhong, Wang, Bill, West, Guenter, Wolf, John, Wooley, and Ian A, Wilson

Subjects
Models, Molecular, Thermotoga maritima, Thymidylate Synthase, Crystallography, X-Ray, Sensitivity and Specificity
Published
2002

35. Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline

Author

Chittibabu Guda, Timothy M. McPhillips, I.I. Mathews, Tanya Shin, Adam Godzik, Jaume M. Canaves, Andreas Kreusch, Marc-André Elsliger, Peter G. Schultz, M. Miller, John Wooley, Alyssa Robb, Lukasz Jaroszewski, Mark A. Miller, Juli Vincent, Daniel McMullan, Raymond C. Stevens, Linda S. Brinen, Peter Kuhn, Heath E. Klock, Daniel Scheibe, Thomas L. Selby, Ian A. Wilson, Keith O. Hodgson, Ashley M. Deacon, Susan S. Taylor, Scott A. Lesley, and Glen Spraggon

Subjects
Models, Molecular, Multidisciplinary, biology, Proteome, Protein Conformation, Pipeline (computing), Computational biology, Biological Sciences, biology.organism_classification, Molecular biology, Aquatic organisms, Structural genomics, Open Reading Frames, Protein structure, Thermotoga maritima, bacteria, Cloning, Molecular, Throughput (business), Genome, Bacterial, Protein Structure Initiative
Abstract

Structural genomics is emerging as a principal approach to define protein structure–function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima . By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.

Published
2002

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