1. Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig
- Author
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Kobayashi, Yoshiomi, Shigyo, Michiko, Platoshyn, Oleksandr, Marsala, Silvia, Kato, Tomohisa, Takamura, Naoki, Yoshida, Kenji, Kishino, Akiyoshi, Bravo-Hernandez, Mariana, Juhas, Stefan, Juhasova, Jana, Studenovska, Hana, Proks, Vladimir, Driscoll, Shawn P, Glenn, Thomas D, Pfaff, Samuel L, Ciacci, Joseph D, and Marsala, Martin
- Subjects
Biological Sciences ,Stem Cell Research - Induced Pluripotent Stem Cell - Human ,Stem Cell Research - Induced Pluripotent Stem Cell ,Neurosciences ,Neurodegenerative ,Transplantation ,Stem Cell Research - Nonembryonic - Human ,Stem Cell Research ,Regenerative Medicine ,Spinal Cord Injury ,Development of treatments and therapeutic interventions ,5.2 Cellular and gene therapies ,Adult ,Animals ,Humans ,Rats ,Cell Differentiation ,Cellular Reprogramming ,Genetic Vectors ,Graft Survival ,Induced Pluripotent Stem Cells ,Injections ,Spinal ,Neural Stem Cells ,Sendai virus ,Specimen Handling ,Stem Cell Transplantation ,Swine ,Tissue and Organ Harvesting ,Treatment Outcome ,Brain ,Spinal Cord ,human induced pluripotent stem cells ,neural precursor cells ,cryopreservation ,spinal cord ,human injection device ,immunosuppressed adult pig ,Technology ,Medical and Health Sciences ,Neurology & Neurosurgery ,Biological sciences - Abstract
One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that are simple to use is critical for potential future dissemination of any spinally targeted cell-replacement therapy into general medical practice. Here, we compared the engraftment properties of established human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cell (NPCs) line once cells were harvested fresh from the cell culture or previously frozen and then grafted into striata or spinal cord of the immunodeficient rat. A newly developed human spinal injection device equipped with a spinal cord pulsation-cancelation magnetic needle was also tested for its safety in an adult immunosuppressed pig. Previously frozen NPCs showed similar post-grafting survival and differentiation profile as was seen for freshly harvested cells. Testing of human injection device showed acceptable safety with no detectable surgical procedure or spinal NPCs injection-related side effects.
- Published
- 2023