1. The Nrd1-like protein Seb1 coordinates cotranscriptional 3′ end processing and polyadenylation site selection
- Author
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Rob van Nues, Jürg Bähler, Jean-François Lemay, Xiaochuan Liu, Samuel Marguerat, Marc Larochelle, Bin Tian, Sander Granneman, François Bachand, Mainul Hoque, and Judit Hunyadkürti
- Subjects
0301 basic medicine ,Cleavage factor ,Saccharomyces cerevisiae Proteins ,Transcription Elongation, Genetic ,Polyadenylation ,Termination factor ,Amino Acid Motifs ,Vesicular Transport Proteins ,RNA polymerase II ,Saccharomyces cerevisiae ,Cleavage and polyadenylation specificity factor ,Biology ,Nrd1 ,03 medical and health sciences ,Protein Domains ,Transcription (biology) ,Schizosaccharomyces ,Genetics ,RNA Processing, Post-Transcriptional ,Psychology And Cognitive Sciences ,transcription termination ,Medical And Health Sciences ,Transcription termination ,Biological Sciences ,3′ end processing ,Post-transcriptional modification ,030104 developmental biology ,Terminator (genetics) ,Seb1 ,biology.protein ,RNA Polymerase II ,Schizosaccharomyces pombe Proteins ,S. pombe ,Research Paper ,Developmental Biology - Abstract
Termination of RNA polymerase II (RNAPII) transcription is associated with RNA 3′ end formation. For coding genes, termination is initiated by the cleavage/polyadenylation machinery. In contrast, a majority of noncoding transcription events in Saccharomyces cerevisiae does not rely on RNA cleavage for termination but instead terminates via a pathway that requires the Nrd1–Nab3–Sen1 (NNS) complex. Here we show that the Schizosaccharomyces pombe ortholog of Nrd1, Seb1, does not function in NNS-like termination but promotes polyadenylation site selection of coding and noncoding genes. We found that Seb1 associates with 3′ end processing factors, is enriched at the 3′ end of genes, and binds RNA motifs downstream from cleavage sites. Importantly, a deficiency in Seb1 resulted in widespread changes in 3′ untranslated region (UTR) length as a consequence of increased alternative polyadenylation. Given that Seb1 levels affected the recruitment of conserved 3′ end processing factors, our findings indicate that the conserved RNA-binding protein Seb1 cotranscriptionally controls alternative polyadenylation.
- Published
- 2016