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Objective: To observe the clinical effect of modified Yu Rong San gel eye mask in the treatment of dark circles. Methods: 96 cases of patients with dark circles were randomly divided into treatment group and control group. In the control group and the treatment group, the common eye mask and the modified Yu Rong San gel eye mask were applied to the black circle area respectively. After 15min, water was used to wash the face, the application of the mask for one time a day, four weeks, one course, and three courses were observed. Results: After three courses of treatment, the total effective rate of the treatment group was higher than that of the control group (P

Lipid-soluble pigments make great contributions to the color of green tea. This study aimed to rapidly and simultaneously measure six main types of lipid-soluble pigments in green tea by using the visible and near-infrared (Vis-NIR) spectroscopy. A total of 135 tea samples with five kinds and three grades were collected for spectral scanning and color measurement, and their lipid-soluble pigments contents were measured by high performance liquid chromatography. It can be found that tea color was closely related to the six pigments. And there were significant differences in lipid-soluble pigments contents among these kinds and grades. Finally, quantitative determination models of the six pigments obtained excellent results with Rp2 of 0.975, 0.973, 0.993, 0.919, 0.962 and 0.965 respectively based on multiple linear regression with the characteristic wavelengths. These results demonstrated that the Vis-NIR spectroscopy combined with chemometrics is a powerful tool for rapid determination of lipid-soluble pigments in green tea.

Guozhen Gao,1,* Wenjun Li,2,* Sha Liu,1,* Dongmei Han,1 Xingwei Yao,1 Juanjuan Jin,1 Dezhi Han,1 Weijing Sun,1 Xiangjun Chen1 1Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia 010051, China; 2Department of Cardio and Nephrology, The 253rd Hospital of PLA, Hohhot, Inner Mongolia 010051, China *These authors contributed equally to this work Purpose: In our previous study, we identified that lncRNA ILF3 antisense RNA 1 (ILF3-AS1) is increased and has oncogenic roles in melanoma. However, the cause of the upregulation of ILF3-AS1 and the modulation between ILF3-AS1 and ILF3 in melanoma are still unknown. This study aimed to investigate the significances of the interaction between ILF3-AS1 and ILF3 in melanoma.Materials and methods: The expression of ILF3 in melanoma tissues and cell lines was measured by quantitative real-time PCR (qRT-PCR). The interactions between ILF3-AS1 and ILF3 were explored by the RNA immunoprecipitation assay, the transcription inhibition assay, qRT-PCR, the chromatin immunoprecipitation assay, and Western blot. Gain-of-function and loss-of-function assays were performed to investigate the effects of ILF3 and ILF3-AS1 on melanoma proliferation, migration, and invasion.Results: ILF3 is also increased in melanoma tissues and cell lines. Increased expression of ILF3 predicts poor survival of melanoma patients. Mechanistic investigation revealed that ILF3 directly binds ILF3-AS1, increases ILF3-AS1 transcript stability, and upregulates ILF3-AS1 transcript levels. ILF3-AS1 represses the binding of EZH2 to the promoter of ILF3, induces euchromatin formation at ILF3 promoter, and activates ILF3 transcription. Thus, ILF3 and ILF3-AS1 form positive feedback loop, which induces the upregulation of ILF3 and ILF3-AS1 in melanoma. The expression of ILF3-AS1 is positively correlated with ILF3 in melanoma tissues. Functional assays revealed that overexpression of ILF3 promotes melanoma proliferation, migration, and invasion. Depletion of ILF3 inhibits melanoma proliferation, migration, and invasion. Moreover, concurrent depletion of ILF3 and ILF3-AS1 significantly suppresses melanoma proliferation, migration, and invasion.Conclusion: Both ILF3-AS1 and ILF3 are increased in melanoma. ILF3-AS1 and ILF3 positively regulate each other. Concurrent targeting ILF3-AS1 and ILF3 has significant tumor-suppressive roles in melanoma. Our data suggested that targeting the positive feedback loop between ILF3 and ILF3-AS1 may be promising therapeutic strategies for melanoma. Keywords: lncRNA, ILF3-AS1, feedback loop, ILF3, melanoma, progression

Cervical cancer is the most common malignant tumor of the female reproductive system. Its incidence rate ranks first among female malignant tumors in China and has been increasing in recent years. However, pelvic lymph node metastasis is an important factor affecting the prognosis of cervical cancer, because pelvic lymph node metastasis determines the scope of surgery, it is also an important basis for postoperative adjuvant treatment. Therefore, an accurate and effective method is needed to detect the presence of lymphatic metastases in time to guide the need for systematic pelvic lymph node dissection. This article explores the clinical application of nano-tracer in the surgical treatment of cervical cancer patients and the impact of postoperative complications on patients with pelvic sentinel lymph nodes. The results show that the success rate of sentinel lymph node tracing in cervical cancer surgery is high, and it is a safe and efficient lymph tracer, which can provide an effective way to study cervical micro metastasis and lymphatic chemotherapy.

With the rapid development of urbanization and mechanization, the contradictions of supply-demand imbalance of urban traffic is increasingly intensifying, particularly the bus supply- demand relationship, which brings about urban problems directly and restricts the socio-economic development. In this paper, a comprehensive systematic analysis on shortcomings of China's existing public transport system was carried out through the public transportation in Hangzhou, then an M2M-based bus information system model was put forward, and the key theory as well as the implementation technology. With the same amount of buses, the proposed M2M-based bus information system can improve people's travel comfort and balance the degree of crowdedness in buses. Except for the urban traffic coordination, it can achieve large-scale flow of passengers in Hangzhou, especially during the rush hours, showing an obvious time and spatial effect, lower the operation cost of urban traffic and cut travel time of passengers. The comprehensive bus coordination system of Hangzhou was discussed through the GPS real-time monitoring of bus information, data processing and the application of bus scheduling information at any time of the vehicle-mounted mobile display device. Corresponding solutions and implementation cases were provided in this paper. Index Terms - public transport system, M2M-based bus information system, urban traffic coordination