Search

Your search keyword '"Juan Sebastian Ramirez‐Prado"' showing total 9 results
Start Over Author "Juan Sebastian Ramirez‐Prado"
9 results on '"Juan Sebastian Ramirez‐Prado"'

1. A new role for histone demethylases in the maintenance of plant genome integrity

Author

Javier Antunez-Sanchez, Matthew Naish, Juan Sebastian Ramirez-Prado, Sho Ohno, Ying Huang, Alexander Dawson, Korawit Opassathian, Deborah Manza-Mianza, Federico Ariel, Cecile Raynaud, Anjar Wibowo, Josquin Daron, Minako Ueda, David Latrasse, R Keith Slotkin, Detlef Weigel, Moussa Benhamed, and Jose Gutierrez-Marcos

Subjects
chromatin, DNA methylation, epimutation, transposon, Medicine, Science, Biology (General), QH301-705.5
Abstract

Histone modifications deposited by the Polycomb repressive complex 2 (PRC2) play a critical role in the control of growth, development, and adaptation to environmental fluctuations of most multicellular eukaryotes. The catalytic activity of PRC2 is counteracted by Jumonji-type (JMJ) histone demethylases, which shapes the genomic distribution of H3K27me3. Here, we show that two JMJ histone demethylases in Arabidopsis, EARLY FLOWERING 6 (ELF6) and RELATIVE OF EARLY FLOWERING 6 (REF6), play distinct roles in H3K27me3 and H3K27me1 homeostasis. We show that failure to reset these chromatin marks during sexual reproduction results in the transgenerational inheritance of histone marks, which cause a loss of DNA methylation at heterochromatic loci and transposon activation. Thus, Jumonji-type histone demethylases play a dual role in plants by helping to maintain transcriptional states through development and safeguard genome integrity during sexual reproduction.

Published
2020
Full Text
View/download PDF

2. MAPK-triggered chromatin reprogramming by histone deacetylase in plant innate immunity

Author

David Latrasse, Teddy Jégu, Huchen Li, Axel de Zelicourt, Cécile Raynaud, Stéphanie Legras, Andrea Gust, Olga Samajova, Alaguraj Veluchamy, Naganand Rayapuram, Juan Sebastian Ramirez-Prado, Olga Kulikova, Jean Colcombet, Jean Bigeard, Baptiste Genot, Ton Bisseling, Moussa Benhamed, and Heribert Hirt

Subjects
Biology (General), QH301-705.5, Genetics, QH426-470
Abstract

Abstract Background Microbial-associated molecular patterns activate several MAP kinases, which are major regulators of the innate immune response in Arabidopsis thaliana that induce large-scale changes in gene expression. Here, we determine whether microbial-associated molecular pattern-triggered gene expression involves modifications at the chromatin level. Results Histone acetylation and deacetylation are major regulators of microbial-associated molecular pattern-triggered gene expression and implicate the histone deacetylase HD2B in the reprogramming of defence gene expression and innate immunity. The MAP kinase MPK3 directly interacts with and phosphorylates HD2B, thereby regulating the intra-nuclear compartmentalization and function of the histone deacetylase. Conclusions By studying a number of gene loci that undergo microbial-associated molecular pattern-dependent activation or repression, our data reveal a mechanistic model for how protein kinase signaling directly impacts chromatin reprogramming in plant defense.

Published
2017
Full Text
View/download PDF

3. CmLHP1 proteins play a key role in plant development and sex determination in melon ( Cucumis melo )

Author

Natalia Yaneth Rodriguez‐Granados, Juan Sebastian Ramirez‐Prado, Rim Brik‐Chaouche, Jing An, Deborah Manza‐Mianza, Sanchari Sircar, Christelle Troadec, Melissa Hanique, Camille Soulard, Rafael Costa, Catherine Dogimont, David Latrasse, Cécile Raynaud, Adnane Boualem, Moussa Benhamed, Abdelhafid Bendahmane, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre of Microbial and Plant Genetics, Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and ANR-17-CE20-0019,EPISEX,Vers la comprehension du controle epigenetique du determinisme du sexe chez les cucurbitaceae(2017)

Subjects
HETEROCHROMATIN PROTEIN-1, FLOWERING TIME, Arabidopsis, sex determination, Plant Development, Plant Science, UNISEXUAL FLOWERS, Histones, cucurbits, LHP1, Cucumis melo, Gene Expression Regulation, Plant, flower development, Genetics, CATALYTIC SUBUNIT, ENCODES, DNA METHYLATION, Plant Proteins, GENE-EXPRESSION, sex allocation, polycomb-mediated gene repression, food and beverages, POLYCOMB-GROUP PROTEINS, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Cell Biology, PRC2, Cucurbitaceae, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding
Abstract

International audience; In monoecious melon (Cucumis melo), sex is determined by the differential expression of sex determination genes (SDGs) and adoption of sex-specific transcriptional programs. Histone modifications such as H3K27me3 have been previously shown to be a hallmark associated to unisexual flower development in melon; yet, no genetic approaches have been conducted for elucidating the roles of H3K27me3 writers, readers, and erasers in this process. Here we show that melon homologs to Arabidopsis LHP1, CmLHP1A and B, redundantly control several aspects of plant development, including sex expression. Cmlhp1ab double mutants displayed an overall loss and redistribution of H3K27me3, leading to a deregulation of genes involved in hormone responses, plant architecture, and flower development. Consequently, double mutants display pleiotropic phenotypes and, interestingly, a general increase of the male:female ratio. We associated this phenomenon with a general deregulation of some hormonal response genes and a local activation of male-promoting SDGs and MADS-box transcription factors. Altogether, these results reveal a novel function for CmLHP1 proteins in maintenance of monoecy and provide novel insights into the polycomb-mediated epigenomic regulation of sex lability in plants.

Published
2022

4. Polycomb-dependent differential chromatin compartmentalization determines gene coregulation in Arabidopsis

Author

Jing An, Javier Antunez-Sanchez, Natalia Y. Rodriguez-Granados, Martin Crespi, David Latrasse, Ying Huang, Juan Sebastian Ramirez-Prado, Magdy M. Mahfouz, Federico Ariel, Lorenzo Concia, Moussa Benhamed, Sanchari Sicar, Deborah Manza-Mianza, Fredy Barneche, Heribert Hirt, Aline V. Probst, José F. Gutierrez-Marcos, Rim Brik-Chaouche, Catherine Bergounioux, Cécile Raynaud, Simon Amiard, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Warwick [Coventry], King Abdullah University of Science and Technology (KAUST), Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Universidad Nacional del Litoral [Santa Fe] (UNL), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Institut Universitaire de France (IUF)China Scholar Council fellowships 201806690005, ANR-19-CE20-0001,3DWheat,Une approche tridimensionnelle de génomique fonctionnelle pour identifier les cibles contrôlant la réponse au stress thermique chez le blé(2019), Raynaud, Cécile, and Une approche tridimensionnelle de génomique fonctionnelle pour identifier les cibles contrôlant la réponse au stress thermique chez le blé - - 3DWheat2019 - ANR-19-CE20-0001 - AAPG2019 - VALID

Subjects
[SDV]Life Sciences [q-bio], macromolecular substances, Interactome, HiChIP, Capture Hi-C, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, Transcription (biology), Arabidopsis, [SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Genetics, Genetics (clinical), ComputingMilieux_MISCELLANEOUS, Epigenomics, Chromatin loops, biology, biology.organism_classification, Chromatin, Cell biology, Chromatin architecture, Polycomb, Histone, biology.protein, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Chromatin Loop, Reprogramming
Abstract

In animals, distant H3K27me3-marked Polycomb targets can establish physical interactions forming repressive chromatin hubs. In plants, growing evidence suggests that H3K27me3 acts directly or indirectly to regulate chromatin interactions, although how this histone modification modulates 3D chromatin architecture remains elusive. To decipher the impact of the dynamic deposition of H3K27me3 on the Arabidopsis thaliana nuclear interactome, we combined genetics, transcriptomics, and several 3D epigenomic approaches. By analyzing mutants defective for histone H3K27 methylation or demethylation, we uncovered the crucial role of this chromatin mark in short- and previously unnoticed long-range chromatin loop formation. We found that a reduction in H3K27me3 levels led to a decrease in the interactions within Polycomb-associated repressive domains. Regions with lower H3K27me3 levels in the H3K27 methyltransferase clf mutant established new interactions with regions marked with H3K9ac, a histone modification associated with active transcription, indicating that a reduction in H3K27me3 levels induces a global reconfiguration of chromatin architecture. Altogether, our results reveal that the 3D genome organization is tightly linked to reversible histone modifications that govern chromatin interactions. Consequently, nuclear organization dynamics shapes the transcriptional reprogramming during plant development and places H3K27me3 as a key feature in the coregulation of distant genes.

Published
2021

5. Histone modification ChIP-seq on Arabidopsis thaliana plantlets

Author

Moussa Benhamed, David Latrasse, Juan Sebastian Ramirez-Prado, Center for Plant Systems Biology (PSB Center), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Universitaire de France (IUF), and ANR-19-CE20-0001,3DWheat,Une approche tridimensionnelle de génomique fonctionnelle pour identifier les cibles contrôlant la réponse au stress thermique chez le blé(2019)

Subjects
biology, Arabidopsis thaliana, Immunoprecipitation, Strategy and Management, Mechanical Engineering, Metals and Alloys, biology.organism_classification, Industrial and Manufacturing Engineering, Chromatin, Cell biology, ChIP-seq, Histone, Gene expression, Methods Article, biology.protein, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Protein–DNA interaction, Chromatin immunoprecipitation, Epigenomics
Abstract

International audience; Characterizing the molecular mechanisms regulating gene expression is crucial for understanding the regulatory processes underlying physiological responses to environmental and developmental signals in eukaryotes. The covalent modification of histones contributes to the compaction levels of chromatin, as well as the recruitment of the transcriptional machinery to specific loci, facilitating metastable changes in gene activity. ChIP-seq (Chromatin Immunoprecipitation followed by sequencing) has become the gold standard method for determining histone modification profiles among different organisms, tissues, and genotypes. In the current protocol, we describe a highly robust method for performing ChIP-seq of histone modifications in Arabidopsis thaliana plantlets. Besides its robustness, this method uses in-house-prepared buffers for chromatin extraction, immunoprecipitation, washing, and elusion, making it cost-effective in contrast to commercial kits.

Published
2021

6. Author response: A new role for histone demethylases in the maintenance of plant genome integrity

Author

Moussa Benhamed, Matthew Naish, Juan Sebastian Ramirez-Prado, Javier Antunez-Sanchez, Detlef Weigel, Minako Ueda, Deborah Manza-Mianza, José F. Gutierrez-Marcos, David Latrasse, Korawit Opassathian, Alexander Dawson, Ying Huang, Sho Ohno, Cécile Raynaud, R. Keith Slotkin, Federico Ariel, Josquin Daron, and Anjar Wibowo

Subjects
Genome integrity, Histone Demethylases, Computational biology, Biology
Published
2020

7. The matrix revolutions: towards the decoding of the plant chromatin three-dimensional reality

Author

Cécile Raynaud, David Latrasse, Ying Huang, Juan Sebastian Ramirez-Prado, Natalia Y. Rodriguez-Granados, Moussa Benhamed, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), This work was funded by the Agence Nationale de la Recherche ANR (3DWheat ANR-19-CE20-0001-01) and (EPICLISPE ANR-18-CE20-0015) projects, and by the Institut Universitaire de France (IUF). YH is funded by the China Scholarship Council (grant agreement 201806690005)., ANR-19-CE20-0001,3DWheat,Une approche tridimensionnelle de génomique fonctionnelle pour identifier les cibles contrôlant la réponse au stress thermique chez le blé(2019), and ANR-18-CE20-0015,EPICLIPSE,Dynamique des marques épigénétiques dans les cellules hôtes infectées et subversion de l'épigénome par le pathogène.(2018)

Subjects
0106 biological sciences, 0301 basic medicine, Transcription factories, transcription factories, ICONS, Physiology, Plant Science, Biology, 01 natural sciences, Genome, Chromosomes, Plant, Chromosome conformation capture, 03 medical and health sciences, Hierarchical organization, Animals, TADs, Gene, Topology (chemistry), 2. Zero hunger, Cell Nucleus, chromosome configuration, food and beverages, ploidy, R-loops, Compartmentalization (psychology), [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, Plants, chromatin loops, Chromatin, 030104 developmental biology, Evolutionary biology, Chromatin conformation, 010606 plant biology & botany
Abstract

In recent years, we have witnessed a significant increase in studies addressing the three-dimensional (3D) chromatin organization of the plant nucleus. Important advances in chromatin conformation capture (3C)-derived and related techniques have allowed the exploration of the nuclear topology of plants with large and complex genomes, including various crops. In addition, the increase in their resolution has permitted the depiction of chromatin compartmentalization and interactions at the gene scale. These studies have revealed the highly complex mechanisms governing plant nuclear architecture and the remarkable knowledge gaps in this field. Here we discuss the state-of-the-art in plant chromosome architecture, including our knowledge of the hierarchical organization of the genome in 3D space and regarding other nuclear components. Furthermore, we highlight the existence in plants of topologically associated domain (TAD)-like structures that display striking differences from their mammalian counterparts, proposing the concept of ICONS—intergenic condensed spacers. Similarly, we explore recent advances in the study of chromatin loops and R-loops, and their implication in the regulation of gene activity. Finally, we address the impact that polyploidization has had on the chromatin topology of modern crops, and how this is related to phenomena such as subgenome dominance and biased gene retention in these organisms.

Published
2020

8. The Polycomb protein LHP1 regulates Arabidopsis thaliana stress responses through the repression of the MYC2‐dependent branch of immunity

Author

Abdelhafid Bendahmane, Ying Huang, Sylvie Citerne, Cécile Raynaud, Maëlle Jaouannet, Deborah Manza-Mianza, Moussa Benhamed, Heribert Hirt, Rim Brik-Chaouche, Juan Sebastian Ramirez-Prado, David Latrasse, Natalia Y. Rodriguez-Granados, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, King Abdullah University of Science and Technology (KAUST), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA)

Subjects
0106 biological sciences, 0301 basic medicine, H3K27me3, [SDV]Life Sciences [q-bio], Mutant, Arabidopsis, Polycomb-Group Proteins, Pseudomonas syringae, Plant Science, drought, 01 natural sciences, chemistry.chemical_compound, stress, Gene Expression Regulation, Plant, Arabidopsis thaliana, Plant Immunity, Abscisic acid, ComputingMilieux_MISCELLANEOUS, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Jasmonic acid, Cell biology, Chromatin, Salicylic Acid, Cyclopentanes, Biology, 03 medical and health sciences, LHP1, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Genetics, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Epigenetics, Oxylipins, Transcription factor, Psychological repression, Plant Diseases, Arabidopsis Proteins, Cell Biology, Ethylenes, biology.organism_classification, Polycomb, 030104 developmental biology, MYC2, aphid, chemistry, Aphids, Transcriptome, 010606 plant biology & botany, pathogen, Abscisic Acid, Transcription Factors
Abstract

International audience; Polycomb repressive complexes (PRCs) have been traditionally associated to the regulation of developmental processes in various organisms, including higher plants. However, similar to other epigenetic regulators, there is accumulating evidence for their role in the regulation of stress and immune-related pathways. In the current study we show that the PRC1 protein LHP1 is required for the repression of the MYC2 branch of jasmonic acid (JA)/ethylene (ET) pathway of immunity. Loss of LHP1 induces the reduction in H3K27me3 levels in the gene bodies of ANAC019 and ANAC055, as well as some of their targets, leading to their transcriptional up-regulation. Consistently, increased expression of these two transcription factors leads to the mis-regulation of several of their genomic targets. The lhp1 mutant mimics the MYC2, ANAC019 and ANAC055 over-expressers in several of their phenotypes, including increased aphid resistance, ABA sensitivity and drought tolerance. In addition, like the MYC2 and ANAC over-expressers, lhp1 displays reduced Salicylic Acid (SA) content caused by a de-regulation of ICS1 and BSMT1, as well as increased susceptibility to the hemibiotrophic pathogen Pseudomonas syringae pv. tomato DC3000. Together, our results indicate that LHP1 regulates the expression of stress responsive genes as well as the homeostasis and responses to the stress hormones SA and ABA. This protein emerges as a key chromatin player fine-tuning the complex balance between developmental and stress-responsive processes.

Published
2019

9. The Arabidopsis APOLO and human UPAT sequence-unrelated long noncoding RNAs can modulate DNA and histone methylation machineries in plants

Author

Camille Fonouni-Farde, Aurélie Christ, Thomas Blein, María Florencia Legascue, Lucía Ferrero, Michaël Moison, Leandro Lucero, Juan Sebastián Ramírez-Prado, David Latrasse, Daniel Gonzalez, Moussa Benhamed, Leandro Quadrana, Martin Crespi, and Federico Ariel

Subjects
Long noncoding RNA, DNA methylation, PRC1, Polycomb, RdDM, Thermomorphogenesis, Biology (General), QH301-705.5, Genetics, QH426-470
Abstract

Abstract Background RNA-DNA hybrid (R-loop)-associated long noncoding RNAs (lncRNAs), including the Arabidopsis lncRNA AUXIN-REGULATED PROMOTER LOOP (APOLO), are emerging as important regulators of three-dimensional chromatin conformation and gene transcriptional activity. Results Here, we show that in addition to the PRC1-component LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), APOLO interacts with the methylcytosine-binding protein VARIANT IN METHYLATION 1 (VIM1), a conserved homolog of the mammalian DNA methylation regulator UBIQUITIN-LIKE CONTAINING PHD AND RING FINGER DOMAINS 1 (UHRF1). The APOLO-VIM1-LHP1 complex directly regulates the transcription of the auxin biosynthesis gene YUCCA2 by dynamically determining DNA methylation and H3K27me3 deposition over its promoter during the plant thermomorphogenic response. Strikingly, we demonstrate that the lncRNA UHRF1 Protein Associated Transcript (UPAT), a direct interactor of UHRF1 in humans, can be recognized by VIM1 and LHP1 in plant cells, despite the lack of sequence homology between UPAT and APOLO. In addition, we show that increased levels of APOLO or UPAT hamper VIM1 and LHP1 binding to YUCCA2 promoter and globally alter the Arabidopsis transcriptome in a similar manner. Conclusions Collectively, our results uncover a new mechanism in which a plant lncRNA coordinates Polycomb action and DNA methylation through the interaction with VIM1, and indicates that evolutionary unrelated lncRNAs with potentially conserved structures may exert similar functions by interacting with homolog partners.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results