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Your search keyword '"Juan Fernando Sorribas-Berjón"' showing total 2 results
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2 results on '"Juan Fernando Sorribas-Berjón"'

1. Analysis of age-related left ventricular collagen remodeling in living donors: Implications in arrhythmogenesis

Author

Laura García-Mendívil, María Pérez-Zabalza, Konstantinos Mountris, Sam Duwé, Nick Smisdom, Marta Pérez, Lluís Luján, Esther Wolfs, Ronald B. Driesen, José María Vallejo-Gil, Pedro Carlos Fresneda-Roldán, Javier Fañanás-Mastral, Manuel Vázquez-Sancho, Marta Matamala-Adell, Juan Fernando Sorribas-Berjón, Javier André Bellido-Morales, Francisco Javier Mancebón-Sierra, Alexánder Sebastián Vaca-Núñez, Carlos Ballester-Cuenca, Aida Oliván-Viguera, Emiliano Diez, Laura Ordovás, and Esther Pueyo

Subjects
Disease, Pathophysiology, Computational bioinformatics, Science
Abstract

Summary: Age-related fibrosis in the left ventricle (LV) has been mainly studied in animals by assessing collagen content. Using second-harmonic generation microscopy and image processing, we evaluated amount, aggregation and spatial distribution of LV collagen in young to old pigs, and middle-age and elder living donors. All collagen features increased when comparing adult and old pigs with young ones, but not when comparing adult with old pigs or middle-age with elder individuals. Remarkably, all collagen parameters strongly correlated with lipofuscin, a biological age marker, in humans. By building patient-specific models of human ventricular tissue electrophysiology, we confirmed that amount and organization of fibrosis modulated arrhythmia vulnerability, and that distribution should be accounted for arrhythmia risk assessment. In conclusion, we characterize the age-associated changes in LV collagen and its potential implications for ventricular arrhythmia development. Consistency between pig and human results substantiate the pig as a relevant model of age-related LV collagen dynamics.

Published
2022
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2. Chronological and biological aging of the human left ventricular myocardium: Analysis of microRNAs contribution

Author

Estel Ramos-Marquès, Elisa Garrido-Huéscar, Juan Carlos Oliveros, Javier André Bellido-Morales, Carlos Ballester-Cuenca, José María Vallejo-Gil, Francisco Javier Mancebón-Sierra, Gorka Muñiz, Javier Fañanás-Mastral, Aida Oliván-Viguera, Manuel F. Jiménez-Navarro, Carlos Gómez-González, Juan Fernando Sorribas-Berjón, Margarita Segovia-Roldan, Hazel Santander-Badules, Alberto Cebollada, Laura García-Mendívil, Maria Perez-Zabalza, Alexánder Sebastián Vaca-Núñez, Jose M. Villaescusa, Esther Pueyo, Marta Matamala-Adell, Laura Ordovás, Emiliano Raúl Diez, Manuel Vázquez-Sancho, Sabarathinam Srinivasan, Pedro Carlos Fresneda-Roldán, Rafael Torres-Pérez, [Ramos-Marquès,E, García-Mendívil,L, Pérez-Zabalza,M, Santander-Badules,H, Srinivasan,S, Garrido-Huéscar,E, Segovia-Roldán,M, Oliván-Viguera,A, Ordovás,L, Pueyo,E] Biomedical Signal Interpretation and Computational Simulation group (BSICoS), Aragón Institute of Engineering Research, University of Zaragoza, Zaragoza, Spain. [Ramos-Marquès,E, Pueyo,E] BSICoS, IIS Aragón, Zaragoza, Spain. [Oliveros,JC, Torres-Pérez,R] Bioinformatics for Genomics and Proteomics, National Center of Biotechnology- Spanish National Research Council, Madrid, Spain. [Cebollada,A] Biocomputation unit, IACS, Zaragoza, Spain. [Vallejo-Gil,JM, Fresneda-Roldán,PC, Fañanás-Mastral,J, Vázquez-Sancho,M, Matamala-Adell,M, Sorribas-Berjón,JF, Bellido-Morales,JA, Mancebón-Sierra,FJ, Vaca-Núñez,AS, Ballester-Cuenca,C] Department of Cardiovascular Surgery, University Hospital Miguel Servet, Zaragoza, Spain. [Jiménez-Navarro,M] Heart Area, Hospital Clínico Universitario Virgen de la Victoria, CIBERCV, IBIMA, Universidad de Málaga, UMA, Málaga, Spain. [Villaescusa,JM] UGC Heart Area, Cardiovascular Surgery Department, Hospital Universitario Virgen de la Victoria de Málaga, Fundación Pública Andaluza para la Investigación de Málaga en Biomedicina y Salud (FIMABIS), CIBERCV Enfermedades Cardiovasculares, Instituto de Salud Carlos III, University of Málaga, Madrid, Spain. [Gómez-González,C, Muñiz,G] Department of Pathology, San Jorge Hospital, Huesca, Spain. [Diez,E] Institute of Experimental Medicine and Biology of Cuyo (IMBECU), CONICET, Mendoza, Argentina. [Ordovás,L] ARAID Foundation, Zaragoza, Spain. [Pueyo,E] Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Zaragoza, Spain, and The Genotype-Tissue Expression (GTEx) Project was supported by the Common Fund of the Office of the Director of the National Institutes of Health, and by NCI, NHGRI, NHLBI, NIDA, NIMH, and NINDS.

Subjects
0301 basic medicine, Male, Aging, Phenomena and Processes::Genetic Phenomena::Phenotype [Medical Subject Headings], Cardiac fibrosis, Envejecimiento, Persons::Persons::Tissue Donors::Living Donors [Medical Subject Headings], Bioinformatics, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Probability::Risk::Risk Factors [Medical Subject Headings], Transcriptome, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], 0302 clinical medicine, CDKN2A, Ventrículos cardíacos, Heart ventricles, Donadores vivos, MicroARNs, microRNA, transcriptomic age marker, Phenotype, Anatomy::Cardiovascular System::Heart::Heart Ventricles [Medical Subject Headings], 3. Good health, medicine.anatomical_structure, Heart aging, Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Computational Biology [Medical Subject Headings], biological aging, Biomarker (medicine), Female, Redes reguladoras de genes, Senescence, gene regulation network, Heart Ventricles, Check Tags::Male [Medical Subject Headings], Phenomena and Processes::Genetic Phenomena::Genetic Structures::Base Sequence::Regulatory Sequences, Nucleic Acid::Gene Regulatory Networks [Medical Subject Headings], Biology, Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Transcription, Genetic::Transcriptome [Medical Subject Headings], 03 medical and health sciences, Biological aging, Phenomena and Processes::Physiological Phenomena::Physiological Processes::Growth and Development::Aging [Medical Subject Headings], medicine, Humans, Living donors, Gene regulation network, Original Paper, Myocardium, biomarkers, Cell Biology, Original Articles, Anatomy::Cardiovascular System::Heart::Myocardium [Medical Subject Headings], medicine.disease, Transcriptoma, Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Antisense Elements (Genetics)::RNA, Antisense::MicroRNAs [Medical Subject Headings], MicroRNAs, 030104 developmental biology, Biomarcadores, Miocardio, Check Tags::Female [Medical Subject Headings], Ventricle, heart aging, Transcriptomic age marker, 030217 neurology & neurosurgery, Biomarkers
Abstract

Aging is the main risk factor for cardiovascular diseases. In humans, cardiac aging remains poorly characterized. Most studies are based on chronological age (CA) and disregard biological age (BA), the actual physiological age (result of the aging rate on the organ structure and function), thus yielding potentially imperfect outcomes. Deciphering the molecular basis of ventricular aging, especially by BA, could lead to major progresses in cardiac research. We aim to describe the transcriptome dynamics of the aging left ventricle (LV) in humans according to both CA and BA and characterize the contribution of microRNAs, key transcriptional regulators. BA is measured using two CA‐associated transcriptional markers: CDKN2A expression, a cell senescence marker, and apparent age (AppAge), a highly complex transcriptional index. Bioinformatics analysis of 132 LV samples shows that CDKN2A expression and AppAge represent transcriptomic changes better than CA. Both BA markers are biologically validated in relation to an aging phenotype associated with heart dysfunction, the amount of cardiac fibrosis. BA‐based analyses uncover depleted cardiac‐specific processes, among other relevant functions, that are undetected by CA. Twenty BA‐related microRNAs are identified, and two of them highly heart‐enriched that are present in plasma. We describe a microRNA‐gene regulatory network related to cardiac processes that are partially validated in vitro and in LV samples from living donors. We prove the higher sensitivity of BA over CA to explain transcriptomic changes in the aging myocardium and report novel molecular insights into human LV biological aging. Our results can find application in future therapeutic and biomarker research., The biological age, measured by transcriptional markers, explains better than the chronological age the transcriptional dynamics of the human aging myocardium. microRNAs that change expression in the aging left ventricle are identified. Such microRNAs potentially regulate genes with cardiac‐specific functions. Cardiac‐enriched microRNAs that increase or decrease with biological age are present in plasma. They are thus candidate biomarkers to represent the biological age of the heart.

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