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Mammalian cell display technology uses eukaryotic protein expression system to display proteins on cell surfaces and has become an important method in biological research. Although mammalian cell display technology has many advantages and development potential, certain attributes of the displayed protein remain uncharacterized, such as whether the displayed proteins re-enter the cell and how displayed proteins move into the cell. Here, we present the endocytosis mechanism, motility behavior, and transport kinetics of displayed proteins determined using HaloTag as the displayed protein and quantum dot-based single-particle tracking. The displayed protein enters the cell through clathrin-mediated endocytosis and is transported through the cell in three stages, which is dependent on microfilaments and microtubules. The dynamic information obtained in this study provides answers to questions about endocytosis and postendocytosis transport of displayed proteins and, therefore, is expected to facilitate the development of surface display technology.

Choledocholithiasis is a severe disorder that affects a significant portion of the world's population. Treatment using endoscopic sphincterotomy (EST) has become widespread; however, recurrence post-EST is relatively common. The bile microbiome has a profound influence on the recurrence of choledocholithiasis in patients after EST; however, the key pathogens and their functions in the biliary tract remain unclear.To investigate the biliary microbial characteristics of patients with recurrent choledocholithiasis post-EST, using next-generation sequencing.This cohort study included 43 patients, who presented with choledocholithiasis at the Guangdong Second Provincial General Hospital between May and June 2020. The patients had undergone EST or endoscopic papillary balloon dilation and were followed up for over a year. They were divided into either the stable or recurrent groups. We collected bile samples and extracted microbial DNA for analysis through next-generation sequencing. Resulting sequences were analyzed for core microbiome and statistical differences between the diagnosis groups; they were examined using the Kyoto Encyclopedia of Genes and Genomes pathway hierarchy level using analysis of variance. Correlation between the key genera and metabolic pathways in bile, were analyzed using Pearson's correlation test.The results revealed distinct clustering of biliary microbiota in recurrent choledocholithiasis. Higher relative abundances (RAs) ofWe demonstrated differences in the bile microbiome of patients with recurrent choledocholithiasis compared to that in patients with stable disease, thereby adding to the current knowledge on its microbiologic etiology.

First, microarc oxidation (MAO) was performed on the surface of the LA103Z magnesium (Mg) alloy. Then, Mg-Al-Co layered double hydroxide (LDH) coatings were grown in situ on the surface of the MAO ceramic coating via hydrothermal treatment at different temperatures. Scanning electron microscopy (SEM), energy-dispersive spectrometry, and x-ray diffraction were used to characterize the microstructure, chemical composition, and phase formation of the composite coatings. The corrosion resistance of the coatings was investigated by the polarization curve and electrochemical impedance spectroscopy. The results indicated that Mg-Al-Co LDH nanosheets covered the surface of the MAO ceramic coating and tended to grow in the recessed area. As the hydrothermal treatment temperature increased, the size of the LDH sheets increased, and the distribution became denser. The electrochemical tests showed that the self-corrosion current density (8.3260 × 10−7 A·cm−2) of the composite coatings prepared at 90 °C possessed optimal corrosion resistance, which was two orders of magnitude lower than that of the MAO ceramic coating (2.3874 × 10−5 A·cm−2). The corrosion resistance of the composite coatings decreased as the preparation temperature increased (100, 110 °C). The Mg-Al-Co LDH coating significantly enhanced the corrosion resistance of the MAO ceramic-coated alloy by sealing holes and microcracks, but high temperature destroyed the internal structure of the composite coatings and reduced their corrosion resistance.

A solution of Al(NO3)3.9H2O and NaOH was used to perform Mg-Al LDH plugging treatment on the LA103Z magnesium-lithium alloy micro-arc oxidation ceramic layer. The effect of treatment temperature on...

The micro-arc oxidization (MAO) ceramic layer on an LA103Z Mg-Li alloy substrate was treated with a hydrothermal treatment at 90 °C for various times. The effect of the hydrothermal treatment time on the microstructure and corrosion behavior of MAO/LDH composite coatings in 3.5 wt.% NaCl solution was investigated, and the mechanism of hydrothermal film formation and corrosion was discussed. The results show that MgO on the surface of the MAO ceramic coating was partially dissolved during the hydrothermal treatment, and the released Mg2+ ions combined with OH− ions in the hydrothermal solution to form Mg(OH)2 nanosheets, which were deposited on the surface of the ceramic coating and its pores. The hydrogen evolution rates of the MAO/LDH composite coatings rank as MAO/LDH-24 h

To improve the corrosion resistance of the ceramic layer prepared by micro-arc oxidation (MAO) on magnesium alloy, a hydrophobic Mg-Al-Co layered double hydroxide (LDH) coating was fabricated on MA...

Using the principles and methods of dendrochronology, we measured tree-ring width of four dominant coniferous species,运用树木年代学的原理和方法,对普达措国家公园大果红杉、长苞冷杉、高山松和麦吊云杉4个优势针叶树种的年轮宽度进行测量,建立年轮宽度差值年表。分析年表与香格里拉气象站的日、月气候数据的相关性,研究4个优势针叶树种的径向生长对气候因子的响应。结果表明: 大果红杉的年生长速率最高,长苞冷杉的年生长速率最低;4种针叶树径向生长对气候因子的响应存在物种特异性,大果红杉与气候因子的相关性最强,麦吊云杉的径向生长对气候因子的响应不敏感;长苞冷杉树轮宽度年表与上年冬季(11、12月)和当年夏季(7月)的平均温度呈显著正相关;大果红杉树轮宽度年表与生长季早期(6月)温度呈显著正相关,与同期降水量和相对湿度呈显著负相关;而高山松树轮宽度年表与生长季早期(5月)的降水量和相对湿度呈显著正相关,与同期最高温度呈显著负相关,表明高山松的径向生长主要受生长季早期水分可利用性的影响。.

The influence of heat treatment from 500 to 1100 °C on the 5 wt.% H2SO4 solution-induced corrosion resistance of high-velocity oxygen-fuel sprayed WC-17Co coatings on 42CrMo steel was investigated, by using x-ray diffractometer (XRD), scanning electron microscopy (SEM)-energy-dispersive spectrometer (EDS), and polarization curve methods. XRD analysis showed decrease in W2C phase intensity with recrystallization of amorphous Co and generation of new Co3W3C and Co6W6C phases with heat treatment. Porosity distribution did not follow a particular pattern; it initially increased and then decreased with increasing temperature. Corrosion resistance sequence of the as-sprayed and heat-treated coatings in 5 wt.% H2SO4 solution was C-5 > C-9 > C-A > C-7 > C-11. Furthermore, microstructure and phase structure of heat-treated coatings revealed the formation of different discontinuous plate-like oxide films on the surface of the heat-treated coatings which indicated the vital effect of binder structure on the corrosion resistance.

Nickel matrix and Si3N4 micron particles were co-deposited on the aluminum alloy by pulse electro-deposition for high temperature performance. Meanwhile, the oxidation resistance was evaluated through the high temperature oxidation test. The phase structure, micrographs and components of the composite coatings were investigated by means of X-ray diffraction (XRD), scanning electron microscopy (SEM) together with energy dispersive spectroscopy (EDS) respectively. The results indicated that Si3N4 particles were uniformly distributed across the coating and there were no pores and cracks or other defects at the coating/substrate interface. Ni-Si3N4 composite coatings are characterized by pyramidal micro-crystallite structure. The thickness of Ni-Si3N4 composite coatings were up to 80 μm for 2h. The results also revealed that the Ni-Si3N4 composite coatings presented better oxidation resistance than the pure Ni coating and aluminum alloy at high temperature. After oxidation at 673 K for 8h, the oxidation resistance of Ni-Si3N4 composite coatings presented the improved oxidation resistance behavior compared to pure Ni and the aluminum alloy, respectively.

Ni-B/Ni-B-B4C composite coatings on the substrate of medium-carbon steel (45#) were successfully fabricated by electroless plating in a low temperature bath. The corrosion resistance of Ni-B coating were examined by using electrochemical workstation. The results indicated that there are no pores and cracks and other defects at the interface between the substrate and composite coating. It was found that the corrosion resistance of Ni-B coating increased and then decreased with the addition of NaBH4 increasing, and the best addition in plating bath was about 0.9 g/L. While the corrosion resistance of Ni-B coating heat-treated at 400°C with NaBH4 addition 0.9 g/L was the worst.

Area-selectively liquid-phase sintering (AS-LPS) method was developed to control the coarsening of Si particles in Al/60vol.% Si composites with the help of sol-gel-derived interfacial modification, achieved by pre-coating discontinuous Al2O3 film on Si powders. The results show that due to the local presence of less than 1 μm-thick films, Si particles can approximately preserve their as-received size and morphology after being sintered at 950 °C. Whereas, for those prepared by use of the unmodified Si powders, Si phases evolve to several hundred-micron-sized flakes, being similar to the typical eutectic Si in Al-Si casting alloys. It is assumed that the Al2O3-film-coated areas at Al/Si interface control the dissolution of Si into Al liquid, while in uncoated areas Al matrix and Si particles bond together by LPS, thus realizing AS-LPS.

The growth of microarc oxidation (MAO) coatings on aluminum substrates was controlled via changing solution temperature from 10 to 60 °C. The results show that an elevation of the temperature lowers the applied voltage and the coating thickness. The coating formed in low-temperature solution demonstrates large-sized pores and contains lots of elements only originating from solute such as P, W, and V. On the contrary, numerous small-sized pores disperse homogeneously on the coating synthesized in high-temperature solution. It is assumed that the variation of solution temperature affects the dissolving capacity of the alkaline solution and the adsorptive capacity of solute anions, thus dominating the growth behaviors of MAO coating.

In this research, micro-hardness and wear resistance of two types of electroless coatings were investigated including Ni-P and Ni-P-B4C composite coatings. Dispersible B4C particles and electroless Ni-P alloy were codeposited on carbon steel by electroless plating and then heat treated at 200, 400 and 600 °C for 1 h, respectively. The cross-section morphology and microstructure of the composite coatings were characterized. Meanwhile, the micro-hardness and tribological behavior of composite coatings were evaluated. The results showed that the Ni-P-B4C composite coating presents better wear resistance in comparison with that of Ni-P coating. The Ni-P-B4C composite coating with heat treated at 400 °C exhibits high micro-hardness and good wear resistance, which the highest hardness is 1200 HV, the minimum wear weight loss is 0.12 mg and the lowest friction coefficient is 0.2054.

To study the microbial community structure on the root surface of patients with periodontitis.Bacterial plaque and tissues from the root neck (RN group),root middle (RM group) and root tine (RT group) of six teeth with mobility 3 in one patient with periodontitis were sampled.The V3V4 region of 16S rRNA was sequenced on the Illumina MiSeq platform.The microbial community structure was analyzed by Mothur,Qiime and SPSS software.The principal component analysis (PCoA) results indicated that the RM samples had a similar microbial community structure as that of the RT samples,which was significant different from that of the RN samples.Thirteen phyla were detected in the three groups of samples,which included 7 dominant phyla.29 dominant genera were detected in 184 genera.The abundance ofThere is region-specificity of microbial community structure on the root surface of patients with periodontitis.

Bilinear derivative method is widely used in calculating multi-soliton solutions of some nonlinear evolution equation. The paper proves some frequently used properties of bilinear derivative from the perspective of the definition of bilinear derivative, hoping to be useful for learning and teaching in nonlinear science.

The annealing treatment of AZ91 cast magnesium alloy was carried out at 415°C for 24h and the alloy was then slowly cooled to room temperature in furnace. As a results, the coarse divorced eutectic phase distributed along the grain boundary dissolve into Mg matrix during the isothermal process, and the thin lamellar β-Mg17Al12 phase precipitates from the magnesium solid solution with a type of pearlite precipitation during furnace cooling. During the cooling process of annealing treatment, the number of β phases as perlite-type were increased with the furnace cooling time. In the early stage of furnace cooling, the β phase nucleated preferentially in the grain near the boundary, only a small quantity of β phase precipitates.In the subsequent cooling process, the rate of precipitate about β phase increased remarked as the time of furnace cooling prolonging, the number of β phase increases dramatically, and the lamellar β phase evenly distributes inside most of the grain.

The microstructure and corrosion behavior of commercial AZ91/AZ91-0.4%Nd alloys were investigated by OM, immersion test and weight-loss method. It was found that the number of Nd element in the AZ91 magnesium alloy has effect on the grain refining efficiency, the coarse β-Mg17Al12phase distributed along the grain boundaries transformed into granular, and the granular or acicular Al3Nd phase precipitated in matrix. The addition of Nd element significantly reduces the corrosion rate of AZ91-0.4%Nd magnesium alloy, as a result the corrosion resistance of alloy was improved obviously.

The corrosion behavior of AZ91-0.4%Nd alloys was investigated by immersion test in 3.5wt.% NaCl at 25°C. The phase compositions and microstructure of the AZ91-0.4%Nd alloy were characterized by XRD and OM, respectively. The results show that the number of Nd element in the AZ91 magnesium alloy has effect on the grain refining efficiency, and the granular or acicular Al3Nd phase precipitate in matrix.The corrosion products of the AZ91-0.4%Nd alloy mainly composed of Mg(OH)2and Al. The addition of Nd element significantly refine the microstructure and improve the compactness of corrosion products of AZ91-0.4%%Nd magnesium alloy, as a result the corrosion resistance of alloy was improved obviously.

The microstructure and element distribution of AZ80 magnesium alloy as-cast and after annealing treatment were studied by OM, SEM, XRD, EDS and TEM. The results show that the coarse divorced eutectic phase distributed along the grain boundary dissolve into Mg matrix during the isothermal process at 415°C for 24h. During the cooling process of annealing treatment, the lameller-shape β-Mg17Al12 nucleated preferentially in the grain near the boundary, then grows towards the neighbor grains with longitudinal direction, which precipitated from magnesium solid solution as perlite-type precipitation. When cooled to room temperature, the lameller-shape β-Mg17Al12 was uniformly distributed in the matrix, and the lamellar spacing inside β phase is about 1-2μm.

The atomic diffusion and mechanical properties of as-cast AZ80 magnesium alloy after solution treatment at different time were studied by OM,SEM,EDS as well as tensile testing. The results show that the coarse β-Mg17Al12 phase distributed along the grain boundaries as net microstructure is almost dissolved after solution treatment, and the content of Al that in the α-Mg matrix is well distributed with the solution time prolonged. Because of the β-Mg17Al12 phase reducing and granulating, the function of precipitates phase strengthening was depressed and the hardness (HB) of alloy dropped obviously. However, the tensile strength(σb ) and elongation(δ) enhanced remarkably and the yield strength (σ0.2) decreased slightly.

To study microbial diversity of peri-implantitis subgingival with high-throughput sequencing, and investigate microbiological etiology of peri-implantitis.Subgingival plaques were sampled from the patients with peri-implantitis (D group) and non-peri-implantitis subjects (N group). The microbiological diversity of the subgingival plaques was detected by sequencing V4 region of 16S rRNA with Illumina Miseq platform. The diversity of the community structure was analyzed using Mothur software.A total of 156 507 gene sequences were detected in nine samples and 4 402 operational taxonomic units (OTUs) were found. Selenomonas, Pseudomonas, and Fusobacterium were dominant bacteria in D group, while Fusobacterium, Veillonella and Streptococcus were dominant bacteria in N group. Differences between peri-implantitis and non-peri-implantitis bacterial communities were observed at all phylogenetic levels by LEfSe, which was also found in PcoA test.The occurrence of peri-implantitis is not only related to periodontitis pathogenic microbe, but also related with the changes of oral microbial community structure. Treponema, Herbaspirillum, Butyricimonas and Phaeobacte may be closely related to the occurrence and development of peri-implantitis.

Granular activated carbon (GAC) was used to remove bromide (Br⁻) and bromate (BrO(3)(-)) from drinking water in both bench- and pilot-scale experiments. The present study aims to minimize BrO(3)(-) formation and eliminate BrO(3)(-) generated during the ozonation of drinking water, particularly in packaged drinking water. Results show that the Br⁻ and BrO(3)(-) levels in GAC-treated water decreased in both bench- and pilot-scale experiments. In the bench-scale experiments, when the empty bed contact time (EBCT) was 5 min, the highest reduction rates of Br(-) in the mineral and ultrapure water were found to be 74.9% and 91.2%, respectively, and those of BrO(3)(-) were 94.4% and 98.8%, respectively. The GAC capacity for Br⁻ and BrO(3)(-) removal increased with the increase in EBCT. Reduction efficiency was better in ultrapure water than in mineral water. In the pilot-scale experiments, the minimum reduction rates of Br⁻ and BrO(3)(-) were 38.5% and 73.2%, respectively.

ϵ-Poly-L-lysine (ϵ-PL) is an L-lysine homopolymer with strong antimicrobial activity, which is generally produced by Streptomyces strains. ϵ-PL is only produced under acidic conditions in liquid culture, and to improve the current understanding of ϵ-PL biosynthesis, the present study was undertaken to investigate the effects of ϵ-PL on its producer Streptomyces ahygroscopicus GIM8, under acidic and neutral conditions. The results indicated that a neutral pH favored ϵ-PL adsorption onto the cells, whereas minimal adsorption occurred at pH 4.0, the maximum pH for ϵ-PL production. At pH 7.0, small amounts of ϵ-PL caused considerable ATP leakage from the cells, which showed increased membrane permeability. Conversely, ATP leakage was inhibited by ϵ-PL at pH 4.0. Transmission electron microscopy investigation indicated that the cytoplasmic membrane was the primary site of ϵ-PL activity at pH 7.0, and that cell shape was maintained. Metabolic activity profiles revealed that ϵ-PL decreased cellular metabolic activity at a relatively low rate at pH 7.0. However, the toxic effect was significantly enhanced at pH 4.0. Based on these data, a mechanism for the effect of ϵ-PL on ϵ-PL-producing cells under neutral and acidic conditions is proposed. Additionally, acidic conditions may potentially be required for ϵ-PL biosynthesis in liquid culture because low pH can increase membrane permeability and prevent binding of ϵ-PL onto cells, both of which favor the secretion of the ϵ-PL produced by the cells into the broth. This research contributes to the current understanding of ϵ-PL biosynthesis.

To establish a method for simultaneous detection of norovirus (NV), rotavirus (RV), astrovirus (AV) and hepatitis A virus (HAV) by multiplex reverse transcription-polymerase chain reaction (RT-PCR).Specific primers of the four viruses were designed based on the high conserved sequences, the reaction system and conditions optimized and the specificity and sensitivity confirmed. The method was then applied to detect the four viruses in clinical samples.The steady detection limits were 100 pg/ml for hepatitis A virus, 50 pg/ml for rotavirus, norovirus and astrovirus respectively. When the developed method was used to detect clinical fecal samples, 62 (48.44%) were identified as rotavirus, 8 (6.25%) as norovirus, 11 (8.59%) as astrovirus and 4 (3.12%) as hepatitis A virus in a total of 128 samples.Data from our study showed that multiplex RT-PCR system could be used to simultaneously detect the four viruses in routine monitoring and risk assessment in disease outbreaks with high specificity and sensitivity.