1. Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells
- Author
-
Hye Won Jeon, Sue Shin, Kyou Sup Han, Eun Youn Roh, Eun Young Song, Kang Sup Yoon, Jong Hyun Yoon, Nam Hee Jung, Dong Soon Lee, Byoung Jae Kim, and Joung Sung Kim
- Subjects
Cell Survival ,medicine.medical_treatment ,Cell Culture Techniques ,Bioengineering ,Applied Microbiology and Biotechnology ,Umbilical cord ,Umbilical Cord ,Andrology ,Immunophenotyping ,Low intensity ultrasound ,medicine ,Humans ,Ultrasonics ,Cell Proliferation ,Chemistry ,Cell growth ,business.industry ,Growth factor ,Mesenchymal stem cell ,Ultrasound ,Mesenchymal Stem Cells ,General Medicine ,Cord lining ,medicine.anatomical_structure ,Immunology ,business ,Biotechnology - Abstract
The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 x 10(5) cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation.
- Published
- 2008