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12. Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface

Author

Voltrova, Barbora, primary, Hybasek, Vojtech, additional, Blahnova, Veronika, additional, Sepitka, Josef, additional, Lukasova, Vera, additional, Vocetkova, Karolina, additional, Sovkova, Vera, additional, Matejka, Roman, additional, Fojt, Jaroslav, additional, Joska, Ludek, additional, Daniel, Matej, additional, and Filova, Eva, additional

Published
2019
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16. The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells

Author

Filova,Elena, Fojt,Jaroslav, Kryslova,Marketa, Moravec,Hynek, Joska,Ludek, Bacakova,Lucie, Filova,Elena, Fojt,Jaroslav, Kryslova,Marketa, Moravec,Hynek, Joska,Ludek, and Bacakova,Lucie

Abstract

Elena Filova,1 Jaroslav Fojt,2 Marketa Kryslova,1 Hynek Moravec,2 Ludek Joska,2 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Czech Republic Abstract: Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties. Keywords: nanostructure, titanium nanotubes, cell adhesion, Saos-2 cells, osteogenic differentiation

Published
2015

28. On the analysis of optical signals from Ti35Nb6Ta and Ti6Al4V surfaces

Author

Penttinen, Niko, Hason, Stanislav, Joska, Ludek, Cvrcek, Ladislav, and Silvennoinen, Raimo

Abstract

Chemical components and initial optical responses of Ti35Nb6Ta alloy are reported. Polished titanium and other titanium alloy Ti6Al4V served as reference surfaces. The chemical composition was determined with an X-ray photoelectron spectroscope (XPS) for the surfaces as well as for water, phosphate buffered saline (PBS) and for human plasma fibrinogen (HPF in PBS) exposed surfaces. The reflectance of the surfaces was modeled utilizing Bruggeman's model, to evaluate the optical changes that the chemical reaction of each liquid can produce. After the model, a diffractive optical element (DOE) based sensor was used to determine the temporal optical signal from the sample surfaces. The coherent and non-coherent signals gathered with DOE sensor were compared to the reflectance model. Exposing to the liquids showed surface oxidation, which could produce lowered reflectance of the surface. The model and the initial temporal responses showed similarities in non-coherent reflectance.

Published
2011
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