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Following the most severe mass extinction event during the Phanerozoic, the Permian-Triassic mass extinction (PTME, ~251.9 Ma), Early Triassic marine fossil communities were thought to be depauperate, poorly diversified, and dominated by abundant and cosmopolitan disaster or opportunistic taxa. Full re-establishment of complex marine ecosystems was thought to have not occurred until, ~8 million years after the PTME, being represented by the Luoping Biota. The highly suppressed Early Triassic marine ecosystem has been thought to be a consequence of recurrent environmental stresses, including high sea surface temperature, episodes of oceanic acidification, and anoxic/euxinic events mainly occurring during the Permian-Triassic transition, the late Dienerian and late Smithian. Alternatively, it can also result from preservation and sampling biases, which are often neglected in many previous works. Here, we report an exceptionally preserved Early Triassic fossil assemblage, the Guiyang Biota, from the Daye Formation near Guiyang, South China. The Guiyang Biota comprises at least 12 classes and 19 orders, including diverse fish fauna and malacostracans, revealing a trophically-complex marine ecosystem. High-precision U-Pb dating shows that the age of the Guiyang Biota is 250.83 +0.07/-0.06 million years ago. This is only 1.08 ± 0.08 million years after the severe Permian-Triassic mass extinction, and this assemblage therefore represents the oldest known Mesozoic lagerstätte so far. The Guiyang Biota indicates the rapid rise of modern-type marine ecosystems after the Permian-Triassic mass extinction.

The conformational state of DNA fine-tunes the transcriptional rate and abundance of RNA. Here we report that DNA G-quadruplex (G4-DNA) accumulates in neurons in an experience-dependent manner, and that this is required for the transient silencing and activation of genes that are critically involved in learning and memory. In addition, site-specific resolution of G4-DNA by dCas9-mediated deposition of the helicase DHX36 impairs fear extinction memory. Dynamic DNA structure states therefore represent a key molecular mechanism underlying memory consolidation.One-Sentence SummaryG4-DNA is a molecular switch that enables the temporal regulation of the gene expression underlying the formation of fear extinction memory.

Circular RNAs (circRNAs) comprise a novel class of regulatory RNAs that are abundant in the brain, particularly within synapses. They are highly stable, dynamically regulated, and display a range of functional roles, including as decoys for miRNAs and proteins and, in some cases, translation. Early work in animal models revealed an association between circRNAs and neurodegenerative and neuropsychiatric disorders; however, relatively few studies have shown a causal link between circRNA function and memory. To address this knowledge gap, we sequenced circRNAs in the synaptosome compartment of the medial prefrontal cortex of fear extinction trained male C57BL/6J mice and found 12837 circRNAs enriched at the synapse, including Cdr1as. Targeted knockdown of Cdr1as in the neural processes of the infralimbic prefrontal cortex of male C57BL/6J mice led to impaired fear extinction memory. Altogether, our findings highlight the importance of localised circRNA activity at the synapse for memory formation and suggest that circRNAs may have a more widespread effect on brain function than previously thought.

Although central to an understanding of Earth's paleogeography and the myriad processes that it affects, the geometry of Pangea during the early phase of its lifetime has remained a topic of contention since the plate tectonic revolution. Despite decades of analysis and discussion, the crux of this debate still largely hinges on sparse, legacy paleomagnetic data derived from early Permian rocks of the Moroccan Meseta. In this work, we present the results of a study designed to revisit, update and expand on those key data, with the provision of new geochronologic and paleomagnetic results from six Permo-Carboniferous basins in central Morocco. New U-Pb zircon ages from volcanic rocks substantiate and refine existing geochronological data and reveal that volcanism among the studied basins spanned approximately 30 Ma, from at least 305 to 277 Ma, but was possibly punctuated by a more intense pulse in the mid-early Permian (~285 Ma). These new U-Pb data furthermore suggest that the age estimates previously assigned to the key poles from the Moroccan Meseta are probably too young, by some ~10 Ma. New paleomagnetic results from six basins yielded a common, well-defined remanent magnetization from 20 sites that is demonstrated to be pre-middle Permian in age. However, it remains unclear whether this magnetization (which is directionally similar to the previous paleomagnetic data from the same basins) is a primary magnetization acquired at ~285 Ma, or a syn-folding remagnetization that was acquired shortly thereafter, at ~275 Ma. In adopting both interpretations as alternative hypotheses, we examine the corresponding paleogeographic implications of both. Through exhaustive comparisons with reference apparent polar wander paths and the direct reconstruction of Gondwana using these new paleomagnetic results, we demonstrate that in either case (i.e. whether the remanent magnetization is primary or was acquired soon after during folding) the data is compatible with a Pangea A geometry during the early Permian. We close with a topical discussion of some persisting arguments used to defend Pangea B and why we consider them to be flawed.

SummaryThe RNA modification N6-methyladenosine (m6A) is critically involved in the regulation of gene activity underlying experience-dependent plasticity, and is necessary for the functional interplay between RNA and RNA binding proteins (RBPs) in the nucleus. However, the complete repertoire of m6A-modified RNA interacting RBPs in the synaptic compartment, and whether they are involved in fear extinction, have yet to be revealed. Using RNA immunoprecipitation followed by mass spectrometry, we discovered 12 novel, synapsespecific, learning-induced m6A readers in the medial prefrontal cortex of male C57/B6 mice. m6A RNA-sequencing also revealed a unique population of learning-related m6A-modified RNAs at the synapse, which includes a variant of the long non-coding RNA (lncRNA) metastasis associated lung adenocarcinoma transcript 1 (Malat1). m6A-modified Malat1 binds to a subset of novel m6A readers, including cytoplasmic FMR1 interacting protein 2 (CYFIP2) and dihydropyrimidase-related protein 2 (DPYSL2) and a cell-type-specific, state-dependent, and synapse-specific reduction in m6A-modified Malat1 disrupts the interaction between Malat1 and DPYSL2 and impairs fear extinction. The consolidation of fear-extinction memory therefore relies on an interaction between m6A-modified Malat1 and select RBPs in the synaptic compartment.

SummaryLong noncoding RNAs (lncRNAs) represent a multidimensional class of regulatory molecules involved in many aspects of brain function. Emerging evidence indicates that lncRNAs are expressed at the synapse; however, a direct role for their activity in this subcellular compartment in memory formation has yet to be demonstrated. Using lncRNA capture-seq on synaptosomes, we identified a significant number of lncRNAs that accumulate at synapses within the infralimbic prefrontal cortex of adult male C57/Bl6 mice. Among these is a splice variant related to the stress-associated lncRNA, Gas5. RNA immunoprecipitation followed by mass spectrometry and single molecule imaging revealed that this Gas5 isoform, in association with the RNA binding proteins G3bp2 and Caprin1, regulates the activity-dependent trafficking and clustering of RNA granules in dendrites. In addition, we found that cell-type-specific, state-dependent, and synapse-specific knockdown of the Gas5 variant led to impaired fear extinction memory. These findings identify a new mechanism of fear extinction that involves the dynamic interaction between local lncRNA activity and the coordination of RNA condensates in the synaptic compartment.

The Precambrian comprises the vast majority of Earth’s history. Preserved archives contain essential information about the first few billion years for planetary evolution of our planet. Despite covering a large part of the history of our planet, these outcrops are not so abundant due to erosion and frequently occur in disparate areas. In order to relate them and to establish a timeline of geological events in a world lacking biochronology, we rely on accurate radio-isotopic age determinations. These are, however, rather scarce and still leave several hundreds of million years long time intervals undated. In this study, we present U-Pb age determinations from volcanic and sedimentary units of the Paleoproterozoic Transvaal Supergroup, South Africa. The Transvaal Supergroup is an exceptionally well preserved sequence and therefore accounts for a very large amount of geochemical data. Due to its capacity to produce large data sets the preferred technique in U-Pb zircon geochronology for ancient sediments is LA-ICP-MS. It allows the aqcuisition of maximum depositional ages (MDA) in a fast way and at a relatively low cost. However, the large analytical uncertainty preclude the temporal resolution to distinguish between different processes in such old rocks. Moreover, the standard dating procedure rarely includes zircon treatment via chemical abrasion to mitigate common problems such as open system behavior due to radioactive decay damage related Pb loss. In consequence, interpreted ages might be severely disturbed and may yield MDA’s that are tens to hundreds of million years too young. As an alternative, the much more work-intensive CA-ID-TIMS technique allows the obtention of more accurate and more precise ages, preferably using zircon grains that have previously been screened for their LA-ICP-MS U-Pb age. Our new combined LA-ICP-MS and CA-ID-TIMS data indicates that the glaciogenic Makganyene Formation has a MDA of ~2.42 Ga. Younger age clusters at around ~2.2 Ga from LA-ICP-MS dating disappear with chemical abrasion and have to be interpreted as artifacts of radiation-damage related Pb loss. These new results have important implications for both environmental evolution during the Neoarchean/Paleoproterozoic, as well as for the regional geology. The Makganyene diamictites are thought to represent the oldest Paleoproterozoic glaciation in South Africa. The data also corroborate the hypothesis that the directly overlying-to-locally-interfingered mafic volcanic Ongeluk Formation is ~200 Ma older than the volcanic rocks ~2250 Ma Hekpoort Formation in the East Transvaal basin. We therefore reject the long-standing correlation between both units, as previously published.We demonstrate that LA-ICP-MS is not capable to provide a robust and reliable MDA’s in ancient clastic sediments. CA-ID-TIMS analysis provides dates of significantly higher accuracy, because the chemical abrasion is minimizing Pb-loss in the crystal. Therefore, for studies relying on U-Pb zircon geochronology, we encourage the application of CA-ID-TIMS in the youngest populations previously identified with the LA-ICP-MS. This is particularly important for establishing reliable maximum depositional ages in sedimentary rocks.

We determine the full set of coefficients for the completely general 4-loop gauge and 3-loop Yukawa $ \beta $-functions for the most general renormalizable four-dimensional theories. Using a complete parametrization of the $ \beta $-functions, we compare the general form to the specific $ \beta $-functions of known theories to constrain the unknown coefficients. The Weyl consistency conditions provide additional constraints, completing the determination., Comment: 27 pages, 519 tensor structures and coefficients

Four voluminous ignimbrites (150–500 km3) erupted in rapid succession at 27 Ma in the central San Juan caldera cluster, Colorado. To reconstruct the timescales and thermal evolution of these magma reservoirs, we used zircon ID-TIMS U–Pb geochronology, zircon LA-ICP-MS geochemistry, thermal modeling, and zircon age and crystallization modeling. Zircon geochronology reveals dispersed zircon age spectra in all ignimbrites, with decreasing age dispersion through time that we term a ‘chimney sweeping’ event. Zircon whole-grain age modeling suggests that 2σ zircon age spans represent approximately one-quarter of total zircon crystallization timescales due to the averaging effect of whole-grain, individual zircon ages, resulting in zircon crystallization timescales of 0.8–2.7 m.y. Thermal and zircon crystallization modeling combined with Ti-in-zircon temperatures indicates that magma reservoirs were built over millions of years at relatively low magmatic vertical accretion rates (VARs) of 2–5 × 10–3 m y−1 (2–5 × 10–6 km3 y−1 km−2), and we suggest that such low VARs were characteristic of the assembly of the greater San Juan magmatic body. Though we cannot unequivocally discern between dispersed zircon age spectra caused by inheritance (xenocrystic or antecrystic) versus prolonged crystallization from the same magma reservoir (autocrystic), our findings suggest that long-term magma input at relatively low VARs produced thermally mature upper crustal magma reservoirs resulting in protracted zircon crystallization timescales. Compiling all U–Pb ID-TIMS zircon ages of large ignimbrites, we interpret the longer timescales of subduction-related ignimbrites as a result of longer term, lower flux magmatism, and the shorter timescales of Snake River Plain ignimbrites as a result of shorter term, higher flux magmatism.

In this talk we discuss recent computations of the top quark mass dependence of QCD amplitudes describing Higgs boson production in gluon fusion. We compute terms in the expansion for a large top quark mass, which reduces the Feynman diagrams to products of massless integrals and massive tadpole integrals which contain the top mass dependence. In particular we discuss the real and virtual corrections to double Higgs production at NNLO, and the virtual corrections to single Higgs production at N3LO., 8 Pages, 6 Figures. Contribution to the 15th International Symposium on Radiative Corrections (RADCOR) and the XIX Workshop on Radiative Corrections for the LHC and Future Colliders (LoopFest)

This essay draws on Cheryl Harris’s essay ‘Whiteness as Property’ to explore the ways in which nineteenth-century nationalist thinking haunts medieval studies. Using the Old English poem Beowulf as its central example, the essay examines how, during its early scholarly history, Beowulf was identified as the property of various nations and peoples. It was the subject of claims and counterclaims, but all the litigants agreed that, whomever the poem might belong to, it revealed important properties of their identity. This essay also argues that the structures of thought that define early Beowulf scholarship continue to haunt aspects of twenty-first century political culture in Britain. It demonstrates how the idea of ‘the English’ is often simplified and reified and illustrates how this way of thinking about the nation – as self-identical, coherent, and unified – is often indebted to medievalist thinking which simplifies and reifies medieval culture and history.

The end-Triassic mass extinction (ETME) is thought to have been caused by voluminous, pulsed volcanic activity of the Central Atlantic Magmatic Province (CAMP). Over the last decades, various geochemical signals and proxy records, including δ13C, pCO2, iridium and other platinum-group elements, mercury, polycyclic aromatic hydrocarbons (PAH), charcoal and SO2, have been directly or indirectly attributed to CAMP magmatism. Here, we compile and discuss these various records in a stratigraphic framework to present a cohesive chain of events for the CAMP and the end-Triassic mass extinction. Mercury and iridium anomalies indicate that CAMP activity commenced prior to the onset of the marine extinctions (as marked by the last occurrence of the Triassic ammonoid Choristoceras marshi or closely related species), and a negative δ13C excursion in organic matter (the Marshi CIE). This CIE may be explained by input of light carbon to the atmosphere from CAMP lavas of the Tiourjdal and Prevalent groups. Pedogenic carbonate below and above the Prevalent group in North America indicates a more than twofold increase in atmospheric pCO2. Subsequent n-alkane C-isotopes, and stomatal pCO2 data seem to indicate a temporary cooling after the Marshi CIE, which is consistent with climate models incorporating volcanic emissions of both CO2 and SO2. Records of excess iridium and Hg/TOC indicate intensified magmatism during the extinction interval. Tectonic and perhaps epeirogenic (i.e. doming due to rise of magma) activity is suggested by the occurrence of multiple and widespread seismites in Europe. Atmospheric pCO2 proxies indicate global warming, which culminated contemporaneously with a second negative CIE (the Spelae CIE) at the level of the first occurrence of the ammonoid Psiloceras spelae, the index taxon fot the Triassic−Jurassic boundary (TJB). Global warming at this level is corroborated by increased wildfire activity testified by charcoal and pyrolytic PAH records. Just prior to the increase in pCO2 from stomatal proxy data, fossil plants exhibit SO2-induced damage indicating excess sulfur dioxide deposition priot to and across the TJB. This coincides with increased ratios of heavy molecular PAHs (coronene/benzo(a)pyrene) in sediments, which may suggest metamorphism of organic sediments also occurred across the TJB. This suggests that thermogenic release of light carbon and sulfur from sill intrusions in the Trans-Amazonian basins, where both evaporate- and organic-rich sediments are known to have been intruded, may have played an important role during the course of the ETME. Geochemical traces of magmatism, i.e. Ir and Hg, appear to have gradually disappeared during the Hettangian, suggesting that later phases of CAMP were less voluminous. Stomatal proxy data from Greenland and n-alkane C-isotope data from the UK, together with oxygen isotope data from carbonate fossils in the UK, may indicate that the global warming at the Spelae CIE was succeeded by another short-term cooling event. A gradual decrease in δ13C culminated at the top-Tilmanni CIE, marking the beginning of a long-term steady state with more negative C-isotope values than prior to the ETME. At this time, terrestrial ecosystems appear to have stabilized globally and ammonoids had begun to rediversify.

The first reported in-situ synchrotron X-ray diffraction experiments for the inertia friction welding process are presented. The evolution of the microstructure around the weld interface has been quantified throughout the process., The widespread use and development of inertia friction welding is currently restricted by an incomplete understanding of the deformation mechanisms and microstructure evolution during the process. Understanding phase transformations and lattice strains during inertia friction welding is essential for the development of robust numerical models capable of determining optimized process parameters and reducing the requirement for costly experimental trials. A unique compact rig has been designed and used in-situ with a high-speed synchrotron X-ray diffraction instrument to investigate the microstructure evolution during inertia friction welding of a high-carbon steel (BS1407). At the contact interface, the transformation from ferrite to austenite was captured in great detail, allowing for analysis of the phase fractions during the process. Measurement of the thermal response of the weld reveals that the transformation to austenite occurs 230 °C below the equilibrium start tem­per­ature of 725 °C. It is concluded that the localization of large strains around the contact interface produced as the specimens deform assists this non-equilibrium phase transformation.

The pH drop in the hindgut of the horse is caused by lactic acid-producing bacteria which are abundant when a horse’s feeding regime is excessively carbohydrate rich. This drop in pH below six causes hindgut acidosis and may lead to laminitis. Lactic acid-producing bacteria Streptococcus equinus and Mitsuokella jalaludinii have been found to produce high amounts of L-lactate and D-lactate, respectively. Early detection of increased levels of these bacteria could allow the horse owner to tailor the horse’s diet to avoid hindgut acidosis and subsequent laminitis. Therefore, 16s ribosomal ribonucleic acid (rRNA) sequences were identified and modified to obtain target single stranded deoxyribonucleic acid (DNA) from these bacteria. Complementary single stranded DNAs were designed from the modified target sequences to form capture probes. Binding between capture probe and target single stranded deoxyribonucleic acid (ssDNA) in solution has been studied by gel electrophoresis. Among pairs of different capture probes and target single stranded DNA, hybridization of Streptococcus equinus capture probe 1 (SECP1) and Streptococcus equinus target 1 (SET1) was portrayed as gel electrophoresis. Adsorptive stripping voltammetry was utilized to study the binding of thiol modified SECP1 over gold on glass substrates and these studies showed a consistent binding signal of thiol modified SECP1 and their hybridization with SET1 over the gold working electrode. Cyclic voltammetry and electrochemical impedance spectroscopy were employed to examine the binding of thiol modified SECP1 on the gold working electrode and hybridization of thiol modified SECP1 with the target single stranded DNA. Both demonstrated the gold working electrode surface was modified with a capture probe layer and hybridization of the thiol bound ssDNA probe with target DNA was indicated. Therefore, the proposed electrochemical biosensor has the potential to be used for the detection of the non-synthetic bacterial DNA target responsible for equine hindgut acidosis.

Zircon occasionally crystallizes in evolved melt pockets in mafic large igneous province (LIP) magmas, and in these cases, it is used to provide high-precision age constraints on LIP events. The precision and accuracy of high-precision ages from LIPs are crucially important, because they may be implicated in mass extinctions. However, why zircon crystallizes in these magmas is not clearly understood, since their mafic compositions should limit zircon saturation. Here, we investigate the occurrence of zircon (and baddeleyite) in intrusive and extrusive mafic rocks from Central Atlantic Magmatic Province (CAMP) using petrography, trace-element analysis, Ti temperatures, Hf and oxygen isotopes, and high-precision U–Pb geochronology, along with petrological and thermal modeling. We provide new ages for CAMP sills that intruded into Paleozoic sediments in Brazil, indicating that the high and low Ti magmatism in this area occurred synchronously over 264 ± 57 ka. We show that upper crustal assimilation, especially of shales, during the emplacement of the CAMP likely led to zircon saturation. Assimilation of upper crustal sediments is also supported by high δ18O values and some rare negative εHf values in the zircon crystals. The only extrusive sample analyzed was the North Mountain basalt in Nova Scotia, Canada. This sample contains a large age variation in its zircon crystals (up to 4 Ma), and the older crystals have slightly more negative εHf values suggesting the presence of small (micron scale) xenocrystic cores associated with very late-stage sediment assimilation. However, the CAMP dataset as a whole suggests that the presence of xenocrystic cores is rare. Assuming no xenocrystic cores, and considering the zircon undersaturated nature of LIP mafic melts, the oldest zircon age clusters in a population should record the magma emplacement (or time when assimilation occurred), and the younger ages in a population are more likely to reflect Pb loss, especially given the high U concentrations of LIP zircon. Our identification of heterogeneous isotopic and elemental compositions in LIP zircon indicates that zircon in these magmas saturate in isolated minute melt pockets just before the system cools below its solidus.

Future precision measurements of Higgs boson decays will determine the branching fraction for the decay into two photons with a precision at the one percent level. To fully exploit such measurements, equally precise theoretical predictions need to be available. To this end we compute four-loop QCD corrections in the large top quark mass expansion to the Higgs boson--photon form factor, which enter the two-photon decay width at next-to-next-to-next-to-leading order. Furthermore we obtain corrections to the two-photon decay width stemming from the emission of additional gluons, which contribute for the first time at next-to-next-to-leading order. Finally, we combine our results with other available perturbative corrections and estimate the residual uncertainty due to missing higher-order contributions., 18 pages, 4 figures

The Antimonio depositional system ranges from the Upper Permian to the uppermost Lower Jurassic. It is the only locality of Upper Triassic shallow marine carbonates in Mexico and through its speculative basement; it provides a potential link between the terrane framework of Mexico and the North American Cordillera. Various paleotectonic models have been proposed to explain the mixed siliciclastic-carbonate successions of the Antimonio depositional system in Sonora, Mexico, including interpreting it as a distinct allochthonous terrane or having a position close to the Laurentian basement. Microfacies, paleontological and paleomagnetic studies have suggested a paleogeographic position in low latitudes close to Laurentia. However, the basement structures of the Antimonio depositional system are poorly known and have numerous uncertainties. Here, we use detrital zircon geochronology to identify the sources of terrestrial sediments of the Antimonio depositional system and to propose potential sedimentary distribution patterns. The results allow us to clarify its paleotectonic position and consolidate the geodynamic model of Gonzalez-Leon et al. (2005), in which the Antimonio depositional system was attached to the Caborca block. Four major zircon age distributions of detrital zircon were determined in the Barra los Tanques and Sierra del Alamo sections of the Rio Asuncion Formation (Antimonio depositional system: (1) a Permo-Triassic group (207–292 Ma); (2) the group/population of 1.0–1.2 Ga; (3) a group clustering between 1.3 and 1.5 Ga and (4) the group of 1.6–1.8 Ga. This new data set was compared with published detrital zircon data from localities along the North American Cordillera, using two-sample Kolmogorov-Smirnov tests and hierarchical clustering. The results allow us to identify potential sources located in the Cordilleran continental arc and the ancestral Rocky Mountains, as well as in sediments and volcanic deposits of their respective surrounding areas. We suggest that the transport for these sediments was likely provided through the Chinle river system, and long-shore oceanic currents. Combining published data from Magsat magnetic anomalies with stratigraphy based paleontology, and our new geochronological data analysis, we suggest that the Antimonio depositional system must have been part of Laurentia and potentially linked to the Caborca block during the Late Triassic.