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277 results on '"Joshi, Amrita"'

3. Macrophage-specific inhibition of the histone demethylase JMJD3 decreases STING and pathologic inflammation in diabetic wound repair

Author

Audu, Christopher O., Melvin, William J., Joshi, Amrita D., Wolf, Sonya J., Moon, Jadie Y., Davis, Frank M., Barrett, Emily C., Mangum, Kevin D., Deng, Hongping, Xing, Xianying, Wasikowski, Rachel, Tsoi, Lam C., Sharma, Sriganesh B., Bauer, Tyler M., Shadiow, James, Corriere, Matthew A., Obi, Andrea T., Kunkel, Steven L., Levi, Benjamin, Moore, Bethany B., Gudjonsson, Johann E., Smith, Andrew M., and Gallagher, Katherine A.

Published
2022
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4. Coronavirus induces diabetic macrophage-mediated inflammation via SETDB2

Author

Melvin, William J., Audu, Christopher O., Davis, Frank M., Sharma, Sriganesh B., Joshi, Amrita, DenDekker, Aaron, Wolf, Sonya, Barrett, Emily, Mangum, Kevin, Zhou, Xiaofeng, Bame, Monica, Ruan, Alex, Obi, Andrea, Kunkel, Steven L., Moore, Bethany B., and Gallagher, Katherine A.

Published
2021

7. Efficacy & safety of brolucizumab 6.0 mg versus 3.6 mg in diabetic macular edema.

Author

Mishra, Sanjay Kumar, Kumar, Pradeep, Joshi, Amrita, Saraf, Aman, Awasthi, Abhijeet, Dhar, Supriya, M, Khaleel, Kumar, Atul, Rana, Vipin, and D, Ravi

Subjects
OPTICAL coherence tomography, MACULAR edema, FOURTH grade (Education), INJECTIONS, MEDICAL sciences
Abstract

Background: Management of Diabetic Macular edema (DME) requires repeated injections. Therefore newer Anti-VEGFs like Brolucizumab with longer durability have been introduced. We compared two different dosages of Brolucizumab, 6.0 mg and 3.6 mg, for their safety & efficacy in treatment of DME, in treatment naïve patients over 52 weeks. Method: A prospective, pilot randomised controlled, single centre, double blinded, two arm comparative study was conducted between Dec 2022 to Apr 2024. The study recruited 82 patients of DME who were randomised into two groups of 41 patients each, one group to be treated with Brolucizumab 6.0 mg in 50 μL and the other to receive 3.6 mg in 30 μL. All patients received the first dose of Brolucizumab at 0 week and were then followed up at every 4 weeks for detailed ophthalmic and OCT macula examination. Those who met the pre-defined re-treatment criteria were re-injected with Brolucizumab, the dose being fixed for each group throughout the study. All patient receiving an injection were further followed up on Day 1, Day 7 and Day 28 to look for any adverse reactions. The efficacy parameters included change in best corrected visusal acuity (BCVA), contrast and central macular thickness (CMT) on Optical Coherence Tomography. The average number of injections recd in each group were also calculated. Results: The change in BCVA from baseline in 6.0 mg group was 0.54 LogMAR units and 3.6 mg group was 0.59 LogMAR units, which was not statistically significant. The reduction in CMT from baseline in 6.0 mg group was 133.2 µm (μ) and 3.6 mg group was 110.6 μ, which was not statistically significant. The improvement in contrast from baseline in 6.0 mg group was 0.74 and 3.6 mg group was 0.95, with p value of 0.0002. The re-injection interval was 14.21 weeks in 6.0 mg group and 15.56 weeks for 3.6 mg subgroup. The total number of adverse events in both groups were similar at 70 in 6.0 mg group and 47 in 3.6 mg group with only one grade 4 adverse event occurring in each group. Conclusion: The results of present study show that the safety and efficacy of both doses of Brolucizumab, i.e. 6.0 mg and 3.6 mg, for treating diabetic macular edema is similar. Trial registration Study was registered with Clinical trials registry of India (CTRI ref no. CTRI/2023/06/054105), registered on 14 Nov 2022. [ABSTRACT FROM AUTHOR]

Published
2025
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10. Diabetic Wound Keratinocytes Induce Macrophage JMJD3-Mediated Nlrp3 Expression via IL-1R Signaling.

Author

Wolf, Sonya J., Audu, Christopher O., Moon, Jadie Y., Joshi, Amrita D., Melvin, William J., Barrett, Emily C., Mangum, Kevin, de Jimenez, Gabriela Saldana, Rocco, Sabrina, Buckley, Sam, Ahmed, Zara, Wasikowski, Rachael, Kahlenberg, J. Michelle, Tsoi, Lam C., Gudjonsson, Johann E., and Gallagher, Katherine A.

Subjects
MYELOID cells, GENE expression, WOUND healing, NLRP3 protein, TISSUE wounds
Abstract

Macrophage (Mφ) plasticity is critical for normal wound repair; however, in type 2 diabetic wounds, Mφs persist in a low-grade inflammatory state that prevents the resolution of wound inflammation. Increased NLRP3 inflammasome activity has been shown in diabetic wound Mφs; however, the molecular mechanisms regulating NLRP3 expression and activity are unclear. Here, we identified that diabetic wound keratinocytes induce Nlrp3 gene expression in wound Mφs through IL-1 receptor–mediated signaling, resulting in enhanced inflammasome activation in the presence of pathogen-associated molecular patterns and damage-associated molecular patterns. We found that IL-1α is increased in human and murine wound diabetic keratinocytes compared with nondiabetic controls and directly induces Mφ Nlrp3 expression through IL-1 receptor signaling. Mechanistically, we report that the histone demethylase, JMJD3, is increased in wound Mφs late post-injury and is induced by IL-1α from diabetic wound keratinocytes, resulting in Nlrp3 transcriptional activation through an H3K27me3-mediated mechanism. Using genetically engineered mice deficient in JMJD3 in myeloid cells (Jmjd3 f/f lyz2 Cre+), we demonstrate that JMJD3 controls Mφ-mediated Nlrp3 expression during diabetic wound healing. Thus, our data suggest a role for keratinocyte-mediated IL-1α/IL-1R signaling in driving enhanced NLRP3 inflammasome activity in wound Mφs. These data also highlight the importance of cell cross-talk in wound tissues and identify JMJD3 and the IL-1R signaling cascade as important upstream therapeutic targets for Mφ NLRP3 inflammasome hyperactivity in nonhealing diabetic wounds. Article Highlights: The molecular mechanism regulating macrophage (Mφ) NLRP3 inflammasome activity in diabetic wounds remains unclear. Diabetic wound keratinocytes induce Nlrp3 gene expression and enhance inflammasome activation in wound Mφs through IL-1 receptor–mediated signaling. IL-1α is increased in human and murine wound diabetic keratinocytes compared with nondiabetic controls and directly induces Mφ Nlrp3 expression via histone demethylase, JMJD3. JMJD3 controls Mφ-mediated Nlrp3 expression during wound healing. [ABSTRACT FROM AUTHOR]

Published
2024
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12. The Histone Methyltransferase Setdb2 Modulates Macrophage Phenotype and Uric Acid Production in Diabetic Wound Repair

Author

Kimball, Andrew S., Davis, Frank M., denDekker, Aaron, Joshi, Amrita D., Schaller, Matthew A., Bermick, Jennifer, Xing, Xianying, Burant, Charles F., Obi, Andrea T., Nysz, Dylan, Robinson, Scott, Allen, Ron, Lukacs, Nicholas W., Henke, Peter K., Gudjonsson, Johann E., Moore, Bethany B., Kunkel, Steve L., and Gallagher, Katherine A.

Published
2019
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14. Reverse nucleus drop: A bizarre phenomenon

Author

Joshi, Amrita, Mishra, Sanjay, Sati, Alok, and Kumar, Sonali

Subjects
Health
Abstract

Byline: Amrita. Joshi, Sanjay. Mishra, Alok. Sati, Sonali. Kumar Case 1 A 60-year-old lady presented to our clinic with diminution of vision for three weeks. On slit-lamp examination, we noted [...]

Published
2023

16. Bandage contact lens - The master masquerader

Author

Joshi, Amrita, Mishra, Sanjay, Sati, Alok, and Kumar, Pradeep

Subjects
Patient compliance, Contact lenses, Health
Abstract

Byline: Amrita. Joshi, Sanjay. Mishra, Alok. Sati, Pradeep. Kumar A 60-year-old gentleman presented to our cornea clinic with a complaint of diminution of vision. On examination, the following were observed: [...]

Published
2022

24. Sepsis Induces Prolonged Epigenetic Modifications in Bone Marrow and Peripheral Macrophages Impairing Inflammation and Wound Healing

Author

Davis, Frank M., Schaller, Matthew A., Dendekker, Aaron, Joshi, Amrita D., Kimball, Andrew S., Evanoff, Holly, Wilke, Carol, Obi, Andrea T., Melvin, William J., Cavassani, Karen, Scola, Melissa, Carson, Beau, Moser, Stephanie, Blanc, Victoria, Engoren, Milo, Moore, Bethany B., Kunkel, Steven L., and Gallagher, Katherine A.

Published
2019
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25. The histone methyltransferase Whsc1 regulates TGFβ-driven macrophage to myofibroblast transition

Author

Mangum, Kevin D, primary, Joshi, Amrita D, additional, Melvin, William J, additional, Mazumder, Purba, additional, Erturk, Asuman, additional, Wolf, Sonya, additional, Barrett, Emily C, additional, Moon, Jadie Yoonjoo, additional, Obi, Andrea, additional, Kunkel, Steven L, additional, Moore, Bethany B, additional, and Gallagher, Katherine, additional

Published
2023
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28. IL-17A-mediated JMJD3 regulation of integrin alpha 3 gene expression alters migration of diabetic keratinocytes and impairs wound repair.

Author

Moon, Jadie Yoonjoo, primary, Wolf, Sonya, additional, Audu, Christopher, additional, Melvin, William, additional, Joshi, Amrita, additional, Mangum, Kevin, additional, Barrett, Emily, additional, Davis, Frank, additional, Mazumder, Purba, additional, Rocco, Sabrina, additional, Erturk, Ilkim, additional, and Gallagher, Katherine, additional

Published
2023
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41. Integration of Edge–AI Into IoT–Cloud Architecture for Landslide Monitoring and Prediction

Author

Joshi, Amrita, Agarwal, Saurabh, Kanungo, Debi Prasanna, and Panigrahi, Rajib Kumar

Abstract

This article presents the development and first-time implementation of an IoT–edge–AI–cloud architecture in an actual landslide location for real-time monitoring and prediction. The proposed architecture benefits the time-critical landslide application by introducing artificial intelligence (AI) and decision-making at the edge of the network. This architecture can address the issues related to network, data packet drops, and device overload while optimizing energy consumption, response latency, and prediction accuracy, all simultaneously. A data offloading scheme is implemented to address the issue of data-packet drops by the IoT-end nodes. This architecture employs an incremental learning approach that periodically retrains the AI model at the edge using real-time data to optimize the prediction accuracy, thus reducing cloud dependency. Compression techniques are also implemented on the edge server to develop light-weighted AI models that can easily run on resource-constrained edge devices.

Published
2024
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45. IFN-κ is critical for normal wound repair and is decreased in diabetic wounds

Author

Wolf, Sonya J., primary, Audu, Christopher O., additional, Joshi, Amrita, additional, denDekker, Aaron, additional, Melvin, William J., additional, Davis, Frank M., additional, Xing, Xianying, additional, Wasikowski, Rachael, additional, Tsoi, Lam C., additional, Kunkel, Steven L., additional, Gudjonsson, Johann E., additional, O’Riordan, Mary X., additional, Kahlenberg, J. Michelle, additional, and Gallagher, Katherine A., additional

Published
2022
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48. Interferon kappa (IFNk) in keratinocytes is critical for normal wound repair and is decreased in diabetic wounds

Author

Wolf, Sonya, primary, Audu, Christopher O, additional, Joshi, Amrita D, additional, denDekker, Aaron, additional, Melvin, William J., additional, Xing, Xianying, additional, Wasikowski, Rachael, additional, Tsoi, Lam C, additional, Gudjonsson, Johann E, additional, Kahlenberg, J Michelle, additional, and Gallagher, Katherine A, additional

Published
2022
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49. Dextran-Mimetic Quantum Dots for Multimodal Macrophage ImagingIn Vivo, Ex Vivo, andIn Situ

Author

Deng, Hongping, primary, Konopka, Christian J., additional, Prabhu, Suma, additional, Sarkar, Suresh, additional, Medina, Natalia Gonzalez, additional, Fayyaz, Muhammad, additional, Arogundade, Opeyemi H., additional, Vidana Gamage, Hashni Epa, additional, Shahoei, Sayyed Hamed, additional, Nall, Duncan, additional, Youn, Yeoan, additional, Dobrucka, Iwona T., additional, Audu, Christopher O., additional, Joshi, Amrita, additional, Melvin, William J., additional, Gallagher, Katherine A., additional, Selvin, Paul R., additional, Nelson, Erik R., additional, Dobrucki, Lawrence W., additional, Swanson, Kelly S., additional, and Smith, Andrew M., additional

Published
2022
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