98 results on '"Joseph Kamau"'
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2. Characterization and Optimization of Coal-Biomass Blends for Syngas Production as Alternative Transport Fuel Sources for Two-Stroke Internal Combustion Engines in Tricycles-Tuk Tuks in Kenya
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Benson Kariuki, Paul Njogu, Joseph Kamau, and Robert Kinyua
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Chemistry ,QD1-999 - Abstract
Conventional fuel sources, such as fossil fuels, have become unaffordable for the low end of the economy and thus the need for sustainable alternatives. Gasification of coal and biomass is considered as an alternative source for small-sized engines, such as tricycles in Kenya. Mui basin coal is a low-quality coal, available in Kenya, and blending with selected biomass (rice husks, Prosopis juliflora, and Hyphaene compressa) presents a new source of alternative transport fuel through cogasification and upgrading. The selected biomass samples were collected, air-dried, and crushed to 60 μm and subjected to calorific determination, proximate, and ultimate analysis. Blending was done at various ratios weight by weight, while thermochemical determinations were conducted using the American Society for Testing and Materials (ASTM). The fixed carbon content was reported as 29.3 ± 5.51% for Mui basin coal, 21.1 ± 1.55% for rice husk, and 31.0 ± 1.00% for H. compressa. Blending was found to improve the hydrogen:carbon ratio with an average rate of 0.12 and decreased the sulphur and nitrogen content, leading to low emissions. The study revealed that calorific values of the fuel sources were as follows: Mui basin coal, 20.41 ± 0.15 MJ/kg; P. juliflora, 18.68 ± 0.17 MJ/kg; and H. compressa, 18.69 ± 0.00 MJ/kg, while rice husks had 12.95 ± 0.1 MJ/kg. Blending was also found to improve calorific values by average values of 0.4 MJ/kg for P. juliflora, 1.3 MJ/kg for H. compressa, and 2.28 MJ/kg for rice husk. The moisture content was reduced by 1–2% for all fuel sources; ash content was reduced by 33%, while the volatile matter was increased by 11% for P. juliflora, 7% for H compressa, and 0.2% for rice husks. The optimal blend of ratio 1 : 1 when cogasified produced syngas, which was upgraded through cleaning and cooling. The upgraded syngas had a moderate ratio of hydrogen to carbon monoxide with the right modification and retrofitting; it can be applied in internal combustion engines of tricycles-“tuk tuks.” The study reveals that the optimal blending ratio of 1 : 1 for coal:biomass reduces the pollution ash content, increases calorific values, and enhances syngas fuel properties to moderate ratio.
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- 2024
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3. Performance enhancement and emissions reduction in a diesel engine using oleander and croton biodiesel doped with graphene nanoparticles
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Treza Wambui, Meshack Hawi, Francis Njoka, and Joseph Kamau
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biodiesel ,compression ignition engine ,emission ,nano additives ,engine performance ,Renewable energy sources ,TJ807-830 - Abstract
Biodiesel is considered a suitable substitute for petroleum diesel because it is renewable, environment-friendly, and has a low carbon footprint. However, its high density, high viscosity and low heating value prevents it from replacing petroleum diesel completely. This study investigates the performance and emission characteristics of a compression ignition engine operating on oleander and croton biodiesel doped with graphene nanoparticles. Five fuel samples are used, including diesel (D100), diesel - 80% blended with oleander and croton biodiesel - 20% (OCB20) and OCB20 dosed with graphene nanoparticles at mass fractions of 50 ppm (mg/L), 75 ppm (mg/L) and 100 ppm (mg/L), respectively. The chemical composition of biodiesel and graphene nanoparticles is analyzed using Fourier Transform Infrared (FTIR) spectroscopy while the morphology of the nanoparticles is analyzed using Scanning Electron Microscope (SEM). Engine tests reveal a significant improvement in brake thermal efficiency, especially at 75 ppm concentration which is 2.76% and 18.93% higher than diesel and OCB20, respectively, and a reduction in brake specific fuel consumption by 2.44% and 16.67% compared to diesel and OCB20, respectively. Carbon monoxide (CO) and unburnt hydrocarbon emissions (UHC) decreases for the 50 ppm sample, recording 8.58% and 21.65% reduction in CO and 52.2% and 50% in UHC compared to the diesel and OCB20, respectively. However, Oxides of Nitrogen (NOx) emissions increase. The results indicate that graphene nanoparticle-enhanced biodiesel can adequately substitute petroleum diesel, albeit with NOx reduction techniques.
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- 2023
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4. Applying an evolutionary mismatch framework to understand disease susceptibility.
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Amanda J Lea, Andrew G Clark, Andrew W Dahl, Orrin Devinsky, Angela R Garcia, Christopher D Golden, Joseph Kamau, Thomas S Kraft, Yvonne A L Lim, Dino J Martins, Donald Mogoi, Päivi Pajukanta, George H Perry, Herman Pontzer, Benjamin C Trumble, Samuel S Urlacher, Vivek V Venkataraman, Ian J Wallace, Michael Gurven, Daniel E Lieberman, and Julien F Ayroles
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Biology (General) ,QH301-705.5 - Abstract
Noncommunicable diseases (NCDs) are on the rise worldwide. Obesity, cardiovascular disease, and type 2 diabetes are among a long list of "lifestyle" diseases that were rare throughout human history but are now common. The evolutionary mismatch hypothesis posits that humans evolved in environments that radically differ from those we currently experience; consequently, traits that were once advantageous may now be "mismatched" and disease causing. At the genetic level, this hypothesis predicts that loci with a history of selection will exhibit "genotype by environment" (GxE) interactions, with different health effects in "ancestral" versus "modern" environments. To identify such loci, we advocate for combining genomic tools in partnership with subsistence-level groups experiencing rapid lifestyle change. In these populations, comparisons of individuals falling on opposite extremes of the "matched" to "mismatched" spectrum are uniquely possible. More broadly, the work we propose will inform our understanding of environmental and genetic risk factors for NCDs across diverse ancestries and cultures.
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- 2023
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5. Draft genome sequences of three emerging beta-lactamase-producing Escherichia coli in the camel production system in Northern Kenya [version 2; peer review: 2 approved]
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Rachael Gachogo, Irene Karegi, Brian Ogoti, Victor Musyoki, Dino Martins, Frank Onyambu, and Joseph Kamau
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Genome Note ,Articles ,E.coli ,beta-lactamase ,AMR ,genome ,whole genome sequencing ,camel - Abstract
We report the draft genome sequences and annotation of three beta-lactamase-producing Escherichia coli ( E.coli) strains isolated from fecal samples of healthy camels in Laikipia county, Kenya. This data adds to the online genome resources to support the ongoing antimicrobial resistance surveillance in the livestock-wildlife interface.
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- 2023
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6. Draft genome sequences of three emerging beta-lactamase-producing Escherichia coli in the camel production system in Northern Kenya [version 2; peer review: 2 approved]
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Brian Ogoti, Dino Martins, Joseph Kamau, Irene Karegi, Victor Musyoki, Rachael Gachogo, and Frank Onyambu
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E.coli ,beta-lactamase ,AMR ,genome ,whole genome sequencing ,camel ,eng ,Medicine ,Science - Abstract
We report the draft genome sequences and annotation of three beta-lactamase-producing Escherichia coli (E.coli) strains isolated from fecal samples of healthy camels in Laikipia county, Kenya. This data adds to the online genome resources to support the ongoing antimicrobial resistance surveillance in the livestock-wildlife interface.
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- 2023
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7. Molecular evidence of Anaplasma phagocytophilum in olive baboons and vervet monkeys in Kenya
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Sophie Jerusa Masika, Gerald Mwangi Muchemi, Tequiero Abuom Okumu, Samson Mutura, Dawn Zimmerman, and Joseph Kamau
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Zoonosis ,Olive baboons ,Vervet monkeys ,Kenya ,Anaplasma phagocytophilum ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Background Nonhuman primates (NHPs) play a significant role in zoonotic spill-overs, serving as either reservoirs, or amplifiers, of multiple neglected tropical diseases, including tick-borne infections. Anaplasma phagocytophilum are obligate intracellular bacteria of the family Anaplasmatacae, transmitted by Ixodid ticks and cause granulocytic anaplasmosis (formerly known as Human Granulocytic Ehrlichiosis (HGE)) in a wide range of wild and domestic mammals and humans too. The aim of this study was to determine whether Anaplasma phagocytophilum was circulating in olive baboons and vervet monkeys in Laikipia County, Kenya. Results Some 146 blood samples collected from olive baboons and 18 from vervet monkeys from Mpala Research Center and Ol jogi Conservancy in Laikipia County were screened for the presence of Anaplasma species using conventional Polymerase Chain Reaction (PCR), and then A. phagocytophilum was confirmed by sequencing using conventional PCR targeting 16S rRNA. This study found an overall prevalence of 18.3% for Anaplasma species. DNA sequences confirmed Anaplasma phagocytophilum in olive baboons for the first time in Kenya. Conclusion This study provides valuable information on the endemicity of A. phagocytophilum bacteria in olive baboons in Kenya. Future research is needed to establish the prevalence and public health implications of zoonotic A. phagocytophilum isolates and the role of nonhuman primates as reservoirs in the region.
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- 2021
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8. Draft genome sequences of three emerging beta-lactamase-producing Escherichia coli in the camel production system in Northern Kenya [version 1; peer review: 1 approved, 1 approved with reservations]
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Rachael Gachogo, Irene Karegi, Brian Ogoti, Victor Musyoki, Dino Martins, Frank Onyambu, and Joseph Kamau
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Genome Note ,Articles ,E.coli ,beta-lactamase ,AMR ,genome ,whole genome sequencing ,camel - Abstract
We report the draft genome sequences and annotation of three beta-lactamase-producing Escherichia coli ( E.coli) strains isolated from fecal samples of healthy camels in Laikipia county, Kenya. This data adds to the online genome resources to support the ongoing antimicrobial resistance surveillance in the livestock-wildlife interface.
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- 2022
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9. Role of Employee Commitment in Total Quality Management on Performance of Institutions of Higher Learning in Kenya
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Joseph, Kamau Njau, primary, Mwenda, Lilian, primary, and Wachira, Anita, primary
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- 2024
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10. Rainfed Rice Farming Production Constrains and Prospects, the Kenyan Situation
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Dianga, Al-Imran, primary, N. Musila, Ruth, additional, and W. Joseph, Kamau, additional
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- 2022
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11. Metagenomic Investigation of Ticks From Kenyan Wildlife Reveals Diverse Microbial Pathogens and New Country Pathogen Records
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Koray Ergunay, Mathew Mutinda, Brian Bourke, Silvia A. Justi, Laura Caicedo-Quiroga, Joseph Kamau, Samson Mutura, Irene Karagi Akunda, Elizabeth Cook, Francis Gakuya, Patrick Omondi, Suzan Murray, Dawn Zimmerman, and Yvonne-Marie Linton
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tick ,metagenomics ,wildlife ,pathogen ,surveillance ,Microbiology ,QR1-502 - Abstract
Focusing on the utility of ticks as xenosurveillance sentinels to expose circulating pathogens in Kenyan drylands, host-feeding ticks collected from wild ungulates [buffaloes, elephants, giraffes, hartebeest, impala, rhinoceros (black and white), zebras (Grévy’s and plains)], carnivores (leopards, lions, spotted hyenas, wild dogs), as well as regular domestic and Boran cattle were screened for pathogens using metagenomics. A total of 75 host-feeding ticks [Rhipicephalus (97.3%) and Amblyomma (2.7%)] collected from 15 vertebrate taxa were sequenced in 46 pools. Fifty-six pathogenic bacterial species were detected in 35 pools analyzed for pathogens and relative abundances of major phyla. The most frequently observed species was Escherichia coli (62.8%), followed by Proteus mirabilis (48.5%) and Coxiella burnetii (45.7%). Francisella tularemia and Jingmen tick virus (JMTV) were detected in 14.2 and 13% of the pools, respectively, in ticks collected from wild animals and cattle. This is one of the first reports of JMTV in Kenya, and phylogenetic reconstruction revealed significant divergence from previously known isolates and related viruses. Eight fungal species with human pathogenicity were detected in 5 pools (10.8%). The vector-borne filarial pathogens (Brugia malayi, Dirofilaria immitis, Loa loa), protozoa (Plasmodium spp., Trypanosoma cruzi), and environmental and water-/food-borne pathogens (Entamoeba histolytica, Encephalitozoon intestinalis, Naegleria fowleri, Schistosoma spp., Toxoplasma gondii, and Trichinella spiralis) were detected. Documented viruses included human mastadenovirus C, Epstein-Barr virus and bovine herpesvirus 5, Trinbago virus, and Guarapuava tymovirus-like virus 1. Our findings confirmed that host-feeding ticks are an efficient sentinel for xenosurveillance and demonstrate clear potential for wildlife-livestock-human pathogen transfer in the Kenyan landscape.
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- 2022
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12. Epidemiological Dynamics of Foot-and-Mouth Disease in the Horn of Africa: The Role of Virus Diversity and Animal Movement
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Fanos Tadesse Woldemariyam, Christopher Kinyanjui Kariuki, Joseph Kamau, Annebel De Vleeschauwer, Kris De Clercq, David J. Lefebvre, and Jan Paeshuyse
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foot-and-mouth disease ,virus diversity ,livestock movement ,wild ungulates ,Horn of Africa ,Microbiology ,QR1-502 - Abstract
The Horn of Africa is a large area of arid and semi-arid land, holding about 10% of the global and 40% of the entire African livestock population. The region’s livestock production system is mainly extensive and pastoralist. It faces countless problems, such as a shortage of pastures and watering points, poor access to veterinary services, and multiple endemic diseases like foot-and-mouth disease (FMD). Foot-and-mouth disease is one of the most economically important livestock diseases worldwide and is endemic in most developing countries. Within Africa, five of the seven serotypes of the FMD virus (FMDV) are described, but serotype C is not circulating anymore, a burden unseen anywhere in the world. The enormous genetic diversity of FMDV is favored by an error-prone RNA-dependent RNA polymerase, intra-typic and inter-typic recombination, as well as the quasi-species nature of the virus. This paper describes the epidemiological dynamics of foot-and-mouth disease in the Horn of Africa with regard to the serotypes and topotypes distribution of FMDV, the livestock production systems practiced, animal movement, the role of wildlife, and the epidemiological complexity of FMD. Within this review, outbreak investigation data and serological studies confirm the endemicity of the disease in the Horn of Africa. Multiple topotypes of FMDV are described in the literature as circulating in the region, with further evolution of virus diversity predicted. A large susceptible livestock population and the presence of wild ungulates are described as complicating the epidemiology of the disease. Further, the husbandry practices and legal and illegal trading of livestock and their products, coupled with poor biosecurity practices, are also reported to impact the spread of FMDV within and between countries in the region. The porosity of borders for pastoralist herders fuels the unregulated transboundary livestock trade. There are no systematic control strategies in the region except for sporadic vaccination with locally produced vaccines, while literature indicates that effective control measures should also consider virus diversity, livestock movements/biosecurity, transboundary trade, and the reduction of contact with wild, susceptible ungulates.
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- 2023
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13. The Importance of Customer Focus on the Institutional Performance of Private Universities in Kenya
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Joseph, Kamau Njau, primary, Wachira, Anita, primary, and Mwenda, Lilian, primary
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- 2024
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14. Norovirus infections and knowledge, attitudes and practices in food safety among food handlers in an informal urban settlement, Kenya 2017
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Eliud Wainaina, Christina A. Otieno, Joseph Kamau, Atunga Nyachieo, and Sara A. Lowther
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Norovirus ,Food handlers ,Informal settlement ,Kenya ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Introduction A leading cause of acute gastroenteritis, norovirus can be transmitted by infected food handlers but norovirus outbreaks are not routinely investigated in Kenya. We estimated norovirus prevalence and associated factors among food handlers in an informal urban settlement in Nairobi, Kenya. Methods We conducted a cross-sectional survey among food handlers using pretested questionnaires and collected stool specimens from food handlers which were analyzed for norovirus by conventional PCR. We observed practices that allow norovirus transmission and surveyed respondents on knowledge, attitudes, and practices in food safety. We calculated odd ratios (OR) with 95% confidence intervals (CI) to identify factors associated with norovirus infection. Variables with p
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- 2020
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15. Ownership Structure, Bank Stability and the Financial Performance of Commercial Banks in South Sudan
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Bak Barnaba Chol, Elizabeth Kalunda Nthambi, and Joseph Kamau
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foreign ownership ,private-local ownership ,public ownership ,joint venture ,financial performance ,Economic history and conditions ,HC10-1085 ,Economic theory. Demography ,HB1-3840 - Abstract
Since independence in 2011, the Republic of South Sudan has witnessed growth in the financial systems and the overall economy. This has led to growth in the number of financial institutions in the country. There is however minimal research on their overall performance. Hence the current research sought to determine the effect of ownership structure, bank stability and the financial performance of commercial banks in South Sudan. The population for the study was all the 29 commercial banks in South Sudan. Secondary data was collected for the period 2012-2017 from audited annual financial reports of individual banks and the Central Bank of South Sudan reports while primary data was collected by the use of a semi-structured questionnaire. Collected data was edited, sorted and coded into SPSS 23 for subsequent data analysis using SPSS 23 statistical analysis tool. This research utilized both descriptive and inferential statistical methods in the analysis. Statistical tests to be utilized in the study included t-tests, f-test, regression models and ANOVA models. Findings of the research were presented using frequencies, percentages, means, standard deviation, correlation coefficients, charts, tables, and other statistical measures. Results of the study indicated there was a statistically significant moderating effect of ownership structure on the financial performance of commercial banks in South Sudan. This study recommends that the government should adopt better measures to safeguard public-owned commercial banks to improve their efficiency and performance.
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- 2020
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16. Author Correction: Predicting the potential for zoonotic transmission and host associations for novel viruses
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Pranav S. Pandit, Simon J. Anthony, Tracey Goldstein, Kevin J. Olival, Megan M. Doyle, Nicole R. Gardner, Brian Bird, Woutrina Smith, David Wolking, Kirsten Gilardi, Corina Monagin, Terra Kelly, Marcela M. Uhart, Jonathan H. Epstein, Catherine Machalaba, Melinda K. Rostal, Patrick Dawson, Emily Hagan, Ava Sullivan, Hongying Li, Aleksei A. Chmura, Alice Latinne, Christian Lange, Tammie O’Rourke, Sarah Olson, Lucy Keatts, A. Patricia Mendoza, Alberto Perez, Cátia Dejuste de Paula, Dawn Zimmerman, Marc Valitutto, Matthew LeBreton, David McIver, Ariful Islam, Veasna Duong, Moctar Mouiche, Zhengli Shi, Prime Mulembakani, Charles Kumakamba, Mohamed Ali, Nigatu Kebede, Ubald Tamoufe, Samuel Bel-Nono, Alpha Camara, Joko Pamungkas, Kalpy J. Coulibaly, Ehab Abu-Basha, Joseph Kamau, Soubanh Silithammavong, James Desmond, Tom Hughes, Enkhtuvshin Shiilegdamba, Ohnmar Aung, Dibesh Karmacharya, Julius Nziza, Daouda Ndiaye, Aiah Gbakima, Zikankuba sajali, Supaporn Wacharapluesadee, Erika Alandia Robles, Benard Ssebide, Gerardo Suzán, Luis F. Aguirre, Monica R. Solorio, Tapan N. Dhole, Nguyen T. T. Nga, Peta L. Hitchens, Damien O. Joly, Karen Saylors, Amanda Fine, Suzan Murray, William B. Karesh, Peter Daszak, Jonna A. K. Mazet, PREDICT Consortium, and Christine K. Johnson
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Biology (General) ,QH301-705.5 - Published
- 2023
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17. A Novel Coronavirus and a Broad Range of Viruses in Kenyan Cave Bats
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Joseph Kamau, Koray Ergunay, Paul W. Webala, Silvia A. Justi, Brian P. Bourke, Maureen W. Kamau, James Hassell, Mary N. Chege, David K. Mwaura, Cynthia Simiyu, Sospeter Kibiwot, Samson Onyuok, Laura Caicedo-Quiroga, Tao Li, Dawn M. Zimmerman, and Yvonne-Marie Linton
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coronavirus ,astrovirus ,retrovirus ,bat ,metagenome ,Kenya ,Microbiology ,QR1-502 - Abstract
Background and Methods: To investigate virus diversity in hot zones of probable pathogen spillover, 54 oral-fecal swabs were processed from five bat species collected from three cave systems in Kenya, using metagenome sequencing. Results: Viruses belonging to the Astroviridae, Circoviridae, Coronaviridae, Dicistroviridae, Herpesviridae and Retroviridae were detected, with unclassified viruses. Retroviral sequences were prevalent; 74.1% of all samples were positive, with distinct correlations between virus, site and host bat species. Detected retroviruses comprised Myotis myotis, Myotis ricketti, Myotis daubentonii and Galidia endogenous retroviruses, murine leukemia virus-related virus and Rhinolophus ferrumequinum retrovirus (RFRV). A near-complete genome of a local RFRV strain with identical genome organization and 2.8% nucleotide divergence from the prototype isolate was characterized. Bat coronavirus sequences were detected with a prevalence of 24.1%, where analyses on the ORF1ab region revealed a novel alphacoronavirus lineage. Astrovirus sequences were detected in 25.9%of all samples, with considerable diversity. In 9.2% of the samples, other viruses including Actinidia yellowing virus 2, bat betaherpesvirus, Bole tick virus 4, Cyclovirus and Rhopalosiphum padi virus were identified. Conclusions: Further monitoring of bats across Kenya is essential to facilitate early recognition of possibly emergent zoonotic viruses.
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- 2022
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18. The intersection of land use and human behavior as risk factors for zoonotic pathogen exposure in Laikipia County, Kenya.
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Joseph Kamau, Elizabeth Ashby, Lindsey Shields, Jennifer Yu, Suzan Murray, Megan Vodzak, Allan Ole Kwallah, Peris Ambala, and Dawn Zimmerman
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Arctic medicine. Tropical medicine ,RC955-962 ,Public aspects of medicine ,RA1-1270 - Abstract
A majority of emerging infectious diseases (EIDs) are zoonotic, mainly caused through spillover events linked to human-animal interactions. We conducted a survey-based human behavioral study in Laikipia County, Kenya, which is characterized by a dynamic human-wildlife-livestock interface. Questionnaires that assessed human-animal interactions, sanitation, and illnesses experienced within the past year were distributed to 327 participants among five communities in Laikipia. This study aimed to 1) describe variation in reported high-risk behaviors by community type and 2) assess the relationship between specific behaviors and self-reported illnesses. Behavioral trends were assessed in R via Fisher's exact tests. A generalized linear mixed model with Lasso penalization (GLMMLasso) was used to assess correlations between behaviors and participants' self-reported illness within the past year, with reported behaviors as independent variables and reported priority symptoms as the outcome. Reported behaviors varied significantly among the study communities. Participants from one community (Pastoralist-1) were significantly more likely to report eating a sick animal in the past year (p< 0.001), collecting an animal found dead to sell in the past year (p
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- 2021
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19. Trend analysis and fatality causes in Kenyan roads: A review of road traffic accident data between 2015 and 2020
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Joseph Kamau Muguro, Minoru Sasaki, Kojiro Matsushita, and Waweru Njeri
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road traffic accidents (rta) ,machine learning ,lda ,kenya ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
With increasing population and motorization, Kenya as well as other African countries are faced with a tragic road traffic accidents (RTA). This paper looks at 5-year (2015–2020) data downloaded from National Transport and Safety Authority (NTSA) website, to identify trends and review progress of the traffic accidents in the country. The objective is to assess the prevalence of accidents within affected groups and location to identify trends and generalized causative agency from the reported data. From literature review, research activity focused on RTA in the country is minimal compared to the social significance accidents poses. The data were extracted and classified using Latent Dirichlet Allocation, a machine learning algorithm modelled in Matlab to group reported accident briefs into general categories/topic which are closely related. Four categories were identified as leading causes of fatality in the country: Knocking down victims, hit-and-run, losing control and head on collision. The identified causes point to preventable driver’s errors which agrees with other researchers. From trend analysis, fatalities and injuries have increased by 26% and 46.5%, respectively since January 2015 to January 2020. This paper found that injuries in vulnerable road users: pedestrians, pillion passengers and motorcyclist, has seen a foldfold increment compared to 2015 data. From the discussion, urgent fine-tuning of policing to protect vulnerable road user as well as curb the overly decried driver behavior is needed. The paper recommends fine-tuning of data collection, capturing details of accident that will be useful in modeling and data analysis for future planning.
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- 2020
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20. Endemicity of Yaws and Seroprevalence of Treponema pallidum Antibodies in Nonhuman Primates, Kenya
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Dawn M. Zimmerman, Emily H. Hardgrove, Michael E. von Fricken, Joseph Kamau, Daniel Chai, Samson Mutura, Velma Kivali, Fatima Hussein, Peris Ambala, Andrea Surmat, Joseph G. Maina, and Sascha Knauf
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Treponema pallidum ,bacteria ,yaws ,antibodies ,baboon ,Kenya ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Human yaws has historically been endemic to Kenya, but current epidemiologic data are lacking. We report seroprevalence for Treponema pallidum antibodies in olive baboons (Papio anubis) and vervet monkeys (Chlorocebus pygerythrus) in Laikipia County, Kenya. Our results suggest endemicity of the yaws bacterium in monkeys, posing a possible zoonotic threat to humans.
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- 2019
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21. Food from faeces: Evaluating the efficacy of scat DNA metabarcoding in dietary analyses.
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David Thuo, Elise Furlan, Femke Broekhuis, Joseph Kamau, Kyle Macdonald, and Dianne M Gleeson
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Medicine ,Science - Abstract
Scat DNA metabarcoding is increasingly being used to track the feeding ecology of elusive wildlife species. This approach has greatly increased the resolution and detection success of prey items contained in scats when compared with other classical methods. However, there have been few studies that have systematically tested the applicability and reliability of this approach to study the diet of large felids species in the wild. Here we assessed the effectiveness of this approach in the cheetah Acinonyx jubatus. We tested how scat degradation, meal size, prey species consumed and feeding day (the day a particular prey was consumed) influenced prey DNA detection success in captive cheetahs. We demonstrated that it is possible to obtain diet information from 60-day old scats using genetic approaches, but the efficiency decreased over time. Probability of species-identification was highest for food items consumed one day prior to scat collection and the probability of being able to identify the species consumed increased with the proportion of the prey consumed. Detection success varied among prey species but not by individual cheetah. Identification of prey species using DNA detection methods from a single consumption event worked for samples collected between 8 and 72 hours post-feeding. Our approach confirms the utility of genetic approaches to identify prey species in scats and highlight the need to account for the systematic bias in results to control for possible scat degradation, feeding day, meal size and prey species consumed especially in the wild-collected scats.
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- 2019
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22. Potential application of DNA barcoding technology for identification of medicinal plant species traded in Nairobi County, Kenya
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Ann Mwaura, Ombori Omwoyo, and Joseph Kamau
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General Mathematics - Abstract
There exists a long history of herbal products use but recently there are concerns on authenticity and safety of some these products. Majority of the herbal products traded in Kenyan markets are processed plant parts, which have been dried or in powder form and are extremely difficult to identify taxonomically. This study was carried out in selected markets in Nairobi County, the capital city of Kenya to assess authenticity of herbal products traded in the region using deoxyribonucleic acid (DNA) barcoding technology by applying internal transcribed spacer (ITS) and ribulose -1,5-bisphosphate carboxylase Large subunit (rbcL) markers. One hundred and twelve herbal products were purchased from practicing herbalists and analyzed using ITS and rbcL DNA barcoding markers. DNA was extracted from acquired samples, amplified and sequences generated. BLAST search with edited generated sequences against the NCBI database was done to recover the expected species. Our findings showed that 35% of the herbal products were wrongly labeled and contained different species compared to those listed on the labels. Out of 92 samples with positive sequences, 58 matched their expected species after the BLAST search. Among the authentic species identified are O. lanceolata, M. oleifera, W. ugandensis, O. europaea, Acacia species, and C. spinarum. However, 32 species did not match the expected species as listed on labels and were concluded to have been substituted. Among them were R. prinoides, T. abyssinica, U. massaica and A. indica, which were substituted with their close relatives, while R. tridentate, Z. usambarense and Prunus africana were substituted with unrelated species with no reported medical benefits. ITS marker had remarkable identification efficiency at 98% to species level compared to rbcL at 89%. We recommend further studies to establish at what stage substitution takes place, as well as utilization of DNA barcoding technology for medicinal plants authentication and verification
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- 2023
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23. Techno-Economic Analysis of a Refractance Window Dryer Prototype Developed by Kenya Industrial Research and Development Institute
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Winstone Asugo Nyaguti, George Wafula Wanjala, Joseph Kamau, and Samuel Warui
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Psychiatry and Mental health ,Engineering ,Architectural engineering ,business.industry ,Industrial research ,Window (computing) ,Techno economic ,business - Abstract
The Refractance window dryer (RWD) is a fourth generation dehydration technology. RWD is used to dry heat-sensitive materials because it retains high nutrient content, colour, flavour, aroma and bioactive compounds. The dehydrated products have a high sensory quality. There were no RWD in East Africa despite their excellent performance hence need for local fabrication of RWD Prototypes that can meet the technical, economical and socio-economical requirements. This paper describes the performance evaluation and economic analysis of the RWD prototype developed at the Kenya Industrial and Development Institute (KIRDI), Kenya. Indicators such as drying rate and drying time were used to assess its technical performance. The economic performance of the dryer was appraised using Net Present Value (NPV), Internal Rate of Return (IRR), Benefit-Cost Ratio (BCR), and Payback Period (PBP). Mango pulp and African leafy vegetables were dried using the RWD prototype at KIRDI between May-July, 2021. During drying process, moisture content of mango pulp was reduced from 88% to 15% in one hour while African leafy vegetable from 88% to 5% in 40 minutes. The economic evaluation of the dryer revealed an IRR of 31%, NPV of 808223.515 @ 24% and PBP of 3.6 years. KIRDI RWD prototype is a versatile technology that can be deployed in remote settings resulting to reduction of post-harvest losses as well as carbon emission. Currently, sun drying, solar drying, cabinet drying, spray drying, drum drying, fluidized bed drying and freeze drying are available in Kenya. They possess various advantages and equally significant drawbacks. Therefore the adoption of KIRDI’s Refractance window dryer would be beneficial to Micro Small and Medium enterprises in providing timely drying services and improve their socio-economic status.
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- 2021
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24. TiO2–CU Thin Film Material for Optical Hydrogen Gas Sensor Applications
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Kamau, Joseph Kamau, primary
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- 2022
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25. Employing multivariate analysis to determine the drivers of productivity on the North Kenya Bank and in Kenyan territorial waters
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Charles Mitto, Shigalla B. Mahongo, Oliver Ochola, Chepkemboi Labatt, Melkizedeck Osore, Boaz Ohowa, Charles Magori, Joseph Kamau, and Margareth S. Kyewalyanga
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Kenya ,Oceanography ,Fishing industry ,Productivity (ecology) ,business.industry ,Ocean current ,Territorial waters ,Environmental science ,Upwelling ,Marine ecosystem ,Monsoon ,business - Abstract
A complex mix of natural processes exist in nearshore and offshore waters which influence coastal and marine ecosystem productivity. An understanding of the biogeochemical processes involved is a key element in interdisciplinary studies of primary production, oceanic flux and storage of carbon dioxide. Water circulation in the East African region is influenced by coastal currents driven by monsoon winds. There are four oceanic currents influencing Kenya’s coastal waters; namely the East African Coastal Current, the Somali Current, the Southern Equatorial Current and the Equatorial Counter Current. The Kenyan fishing industry is slowly embracing offshore fishing grounds, and the North Kenya Bank is emerging as the next fishery frontier. This study aims to provide insight on the processes driving the productivity of Kenya’s territorial waters. The variable Si* (the difference between available silicate [Si(OH)4] and nitrate [NO3-]) was employed as a proxy of upwelling. It was highly positively correlated to chlorophyll-a, indicating that upwelling is a major phenomenon driving productivity in Kenyan territorial waters. Particulate Organic Carbon (POC) and Dissolved Oxygen (DO) exhibited a lesser positive correlation with chlorophyll-a, implying that remineralization also has some influence in the productivity of the area.
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- 2021
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26. Livelihood impacts and adaptation in fishing practices as a response to recent climatic changes in the upwelling region of the East African Coastal Current
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Shigalla B. Mahongo, Joseph Kamau, Faith Kimanga, Fridah Munyi, Jacob Ochiewo, Edward Waiyaki, and Nicholas John Karani
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0106 biological sciences ,010504 meteorology & atmospheric sciences ,Descriptive statistics ,010604 marine biology & hydrobiology ,Fishing ,Vulnerability ,Climate change ,Pelagic zone ,Monsoon ,Livelihood ,01 natural sciences ,Geography ,Upwelling ,Socioeconomics ,0105 earth and related environmental sciences - Abstract
A socio-economic assessment was carried out at Amu and Shela in Lamu County and Ngomeni in Kilifi County on the coast of Kenya. The aim was to establish fisher perspectives on the livelihood impacts of changes in upwelling associated with the East African Coastal Current, and adaptations in fishing practices to determine the vulnerability, resilience and adaptation options for fisheries dependent communities in this upwelling region. Primary data and information were collected through direct observation, semi-structured interviews, key informant interviews and oral histories. Descriptive and non-parametric analysis was conducted for quantitative data and content analysis for qualitative data. The study covered 92 respondents out of which 90 were male. About 82.5 percent of the respondents had attained different levels of primary school education and below, and were therefore highly vulnerable to climate change impacts. Furthermore, 80.4 percent of the respondents were aged between 20 years and 49 years with a mean age of 40 years, thus falling into the economically active age category. In terms of livelihoods, fishing and fishing-related activities formed the primary livelihoods at the three study sites with fishing being the main occupation for 93 percent of the respondents. Fishing effort was higher during the north-east monsoon season. Fifty two percent of the respondents targeted small pelagic species. The main changes observed included increased fishing effort and a decline in the quantity of fish caught per fisher, and changes in the composition of fish species. Changes in the composition of fish species have further been compounded by a decline in rainfall over time, sea level rise, irregular wind patterns and increased temperatures. The decline in fish catch further led to a general decline in income and welfare. The climatic changes increased vulnerability of the fishing communities.
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- 2021
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27. Factors influencing spatial patterns in primary productivity in Kenyan territorial waters
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Boaz Ohowa, James Kilionzi, Amon Kimeli, Shigalla B. Mahongo, Edward Kimani, Melckzedeck K. Osore, Charles Mitto, Noah Ngisiang’e, Oliver Ochola, Harrison Ong'anda, Joseph Kamau, and Charles Magori
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0106 biological sciences ,geography ,geography.geographical_feature_category ,010504 meteorology & atmospheric sciences ,010604 marine biology & hydrobiology ,Pelagic zone ,01 natural sciences ,chemistry.chemical_compound ,Oceanography ,Nutrient ,Nitrate ,chemistry ,Productivity (ecology) ,Archipelago ,Territorial waters ,Environmental science ,Upwelling ,Mangrove ,0105 earth and related environmental sciences - Abstract
This study was formulated to investigate productivity systems within Kenyan territorial waters. The interaction of processes on the margins of the marine waters, particularly the influx of fresh water loaded with sediments and nutrients, influences productivity of coastal waters. These deposited sediments, rich in nutrients, create a topographic barrier to the northerly flowing East African Coastal Current (EACC). Phosphate and nitrate peaks observed around the North Kenya Bank area provide evidence of an upwelling event. The contribution of sediments from the Lamu archipelago mangrove system is evident from the high observed particulate organic carbon (POC) input around the area. The system around the Lamu archipelago did not however show high chlorophyll-a levels despite the high POC influx. This may be due to the low levels of limiting phosphate in the surrounding waters, contrary to the observation further north in the region where high chlorophyll-a levels and corresponding higher phosphate levels were apparent. Productivity was largely supported by upwelling and organic matter mineralization. High levels of chlorophyll corresponded to high pelagic fish densities in the south (around 4.5°S) and north of the study area (around 2.5°S).
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- 2021
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28. Agronomic Performance and Carotenoid Content of Kenyan Yellow-Fleshed Cassava Clones
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Peninah, Njenga, primary, Richard, Edema, additional, Joseph, Kamau, additional, and George, Abong’, additional
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- 2022
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29. Morphological and molecular characterization of cultivated yam (Dioscorea species) in selected counties in Kenya
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Morris Muthini, Grace W. Gatheri, Joseph Kamau, and Valentine Atieno
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0106 biological sciences ,biology ,Phylogenetic tree ,Species distribution ,Dendrogram ,General Medicine ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Phylogenetics ,Genotype ,Botany ,Dioscorea ,010606 plant biology & botany - Abstract
This study was conducted to characterize Dioscorea spp. in Kenya using morphological and molecular characteristics. Data on 22 morphological traits were subjected to cluster analysis and multivariate analysis using principal component (PCA). The dendrogram of cluster analysis revealed three main groups: Species distribution based on PC-1 and PC-2 showed the distantly related species in each quarter; D. alata L. (1st quarter), D. bulbifera L. (2nd quarter), D. cayenensis Lam. (3rd quarter) and D. minutiflora Engl. (4th quarter). In molecular characterization, one sub-cluster grouped D. minutiflora Engl. and D. burkilliana J. Miege as one genetic group. However not all D. minutiflora Engl. species were in one specific cluster showing that there may be variation within the species. D. alata L. and D. bulbifera were seen to be potentially related because they shared a common origin. D. bulbifera L. and D. cayenensis Lam. genotypes clustered together, indicating that the species might be closely related. Generally, the rbcL marker demonstrated the phylogeny of Kenyan Dioscorea spp L. Comparison of morphological and molecular data analysis gave almost similar results. From the study, the phylogenetic relationships of Kenyan Dioscorea spp. were established and morphological and molecular characterization was efficient in establishing species relatedness among Dioscorea spp. Key words: Dioscorea spp., rbcL, principal component analysis, molecular characterization, morphological characterization, yams.
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- 2020
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30. Norovirus infections and knowledge, attitudes and practices in food safety among food handlers in an informal urban settlement, Kenya 2017
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Sara A. Lowther, Atunga Nyachieo, Christina A. Otieno, Joseph Kamau, and Eliud Wainaina
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Adult ,Male ,Hand washing ,medicine.medical_specialty ,Health Knowledge, Attitudes, Practice ,Food Safety ,Food handlers ,Adolescent ,Cross-sectional study ,Food Handling ,media_common.quotation_subject ,medicine.disease_cause ,Disease Outbreaks ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Hygiene ,Environmental health ,medicine ,Humans ,030212 general & internal medicine ,media_common ,Caliciviridae Infections ,0303 health sciences ,030306 microbiology ,business.industry ,Public health ,lcsh:Public aspects of medicine ,Norovirus ,Informal settlement ,Public Health, Environmental and Occupational Health ,lcsh:RA1-1270 ,Middle Aged ,Food safety ,Kenya ,Diarrhea ,Cross-Sectional Studies ,Female ,Biostatistics ,medicine.symptom ,business ,Research Article - Abstract
Introduction A leading cause of acute gastroenteritis, norovirus can be transmitted by infected food handlers but norovirus outbreaks are not routinely investigated in Kenya. We estimated norovirus prevalence and associated factors among food handlers in an informal urban settlement in Nairobi, Kenya. Methods We conducted a cross-sectional survey among food handlers using pretested questionnaires and collected stool specimens from food handlers which were analyzed for norovirus by conventional PCR. We observed practices that allow norovirus transmission and surveyed respondents on knowledge, attitudes, and practices in food safety. We calculated odd ratios (OR) with 95% confidence intervals (CI) to identify factors associated with norovirus infection. Variables with p Results Of samples from 283 respondents, 43 (15.2%) tested positive for norovirus. Factors associated with norovirus detection were: reporting diarrhea and vomiting within the previous month (AOR = 5.7, 95% CI = 1.2–27.4), not knowing aerosols from infected persons can contaminate food (AOR = 6.5, 95% CI = 1.1–37.5), not knowing that a dirty chopping board can contaminate food (AOR = 26.1, 95% CI = 1.6–416.7), observing respondents touching food bare-handed (AOR = 3.7, 95% CI = 1.5–11.1), and working in premises without hand washing services (AOR = 20, 95% CI = 3.4–100.0). Conclusion The norovirus infection was prevalent amongst food handlers and factors associated with infection were based on knowledge and practices of food hygiene. We recommend increased hygiene training and introduce more routine inclusion of norovirus testing in outbreaks in Kenya.
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- 2020
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31. Proximate analysis of fruits and vegetables wastes from Nairobi County, Kenya
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Joseph M. Mwaniki, Francis B. Mwaura, Damaris Mbui, and Joseph Kamau
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Toxicology ,Moisture ,Proximate analysis ,Fruits and vegetables ,General Earth and Planetary Sciences ,Environmental science ,Biomass ,Comfrey ,Proximate ,Water content ,General Environmental Science - Abstract
Proximate analysis of twenty fruits and vegetable waste from Nairobi County was evaluated. They were obtained from Kangemi and Wakulima markets. Standard procedures were used for the analysis of crude fat, protein, fiber, carbohydrates, moisture, ash, nitrogen-free extract and energy. The results obtained revealed that moisture content was in the range of 82.8 to 95.86% apart from sweet potato and banana which was 62.05 and 74.30% respectively. Protein range was between 0.57 to 3.49% with high-fat content being recorded in avocado at 9.03%. The ash content was highest in comfrey at 3.46% and lowest in mango at 0.44%. The carbohydrate level obtained by the difference method was lowest in courgette at 1.99% with crude fiber ranging from 0.69 to 2.73%. The total calculated energy ranged from 1.94 to 39.98 Kcal/100g. The macro-nutrient concentrations were 3.59 and 1.53% for potassium and calcium respectively. Lead, iron and zinc were detected at 15.1±3.6, 3742±235 and 176±11 ppm respectively. There is the presence of proximate properties in the edible portion of wasted fruits and vegetable and therefore, this study recommends proper fruits and vegetable handling during harvest, transportation, storage and marketing. Besides, the unavoidable waste should be used as biomass in energy production to deal with landfilling issues in the market places.
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- 2020
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32. Absence of molecular evidence of filovirus circulating in bats and rodents in Laikipia North Sub-county, Kenya: a cross sectional study
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Peris Ambala, Fatima Hussein, Samson Mutura, George Gachara, Nelson Menza, Lindsey McCrickard Shields, Megan Vodzak, Susan Murray, Marc Valitutto, Dawn Zimmerman, and Joseph Kamau
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- 2022
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33. Evaluation of the detection of staA, viaB and sopE genes in Salmonella spp. using the polymerase chain reaction (PCR)
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Francis Kariuki, Pauline Getanda, Atunga Nyachieo, Gerald Juma, Peter Kinyanjui, and Joseph Kamau
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Salmonella ,Escherichia coli ,Genetics ,General Medicine ,Serogroup ,Kenya ,Polymerase Chain Reaction ,Molecular Biology ,Biochemistry ,Microbiology - Abstract
Typhoid fever is caused by the bacteria Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) and remains a significant health problem in many developing countries. Lack of adequate diagnostic capabilities has contributed greatly in making typhoid fever endemic in these regions. Reliable and inexpensive diagnostic tests are needed to improve the management of this disease burden. We evaluated the ability of staA, viaB and sopE genes to detect and differentiate between the three most prevalent Salmonella spp. in Kenya (S. Typhi, S. Typhimurium and S. Enteritidis) using conventional polymerase chain reaction (PCR). The staA primers and viaB primers were found to be specific only for the different strains of S. Typhi, producing PCR products of 585 bp and 540 bp, respectively. The sopE primers was demonstrated to be specific for all Salmonella spp. producing a 465 bp PCR product with no amplification with E. coli and S. boydii bacterial strains.
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- 2021
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34. Large projected reductions in marine fish biomass for Kenya and Tanzania in the absence of climate mitigation
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Gladys M. Okemwa, Said S. Mgeleka, Johnstone O. Omukoto, Warwick H. H. Sauer, Melita Samoilys, Joseph S. Sululu, Zoe Jacobs, Kennedy Osuka, Sévrine F. Sailley, Robert J. Wilson, Joseph Kamau, Mathew Ogalo Silas, and Michael J. Roberts
- Subjects
Fishery ,Biomass (ecology) ,Effects of global warming ,Fisheries and climate change ,Maximum sustainable yield ,Fishing ,Environmental science ,Climate change ,Exclusive economic zone ,Management, Monitoring, Policy and Law ,Aquatic Science ,Oceanography ,Livelihood - Abstract
Climate change is projected to cause significant reductions in global fisheries catch during the 21st Century. Yet, little is understood of climate change impacts on tropical fisheries, which support many livelihoods, as is the case in the Western Indian Ocean region (WIO). Here, we focus on two central WIO countries ― Kenya and Tanzania ― and run a multi-species fish model (Size Spectrum Dynamic Bio-climate Envelope Model; SS-DBEM) for 43 species of commercial and artisanal importance, to investigate the effects of climate change. We include both national Exclusive Economic Zones (EEZs) as domains. The model was forced by data from a biogeochemical model (NEMO-MEDUSA), run under the high emissions scenario Representative Concentration Pathway (RCP) 8.5, until the end of the 21st century. Impacts of fisheries and climate change were investigated by running SS-DBEM under five scenarios of fishing pressures to predict a range of possible future scenarios. Fishing pressure was represented as the Maximum Sustainable Yield (MSY), expressed as MSY0, MSY1, MSY2, MSY3 and MSY4 representing fishing mortality of 0, 1, 2, 3 and 4 times MSY, respectively. Large reductions in average fish biomass were projected over the 21st Century, with median reductions of fish species biomass of 63–76% and 56–69% for the Kenyan and Tanzanian EEZs respectively across the fishing scenarios. Tunas were particularly impacted by future climate change, with the six modelled species exhibiting biomass reductions of at least 70% in both EEZs for all fishing scenarios during the 21st Century. Reductions in fish biomass were much more severe during the second half of the 21st Century, highlighting the benefits to tropical fisheries of global action on climate change.
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- 2021
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35. 自律走行車での3D ARエンターテインメント時のドライバーの危険状況に対する反応の評価
- Author
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MUGURO JOSEPH KAMAU
- Published
- 2021
36. Microplastics in Kenya's marine nearshore surface waters: Current status
- Author
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Charles Mitto Kosore, Loice Ojwang, Justin Maghanga, Joseph Kamau, Daniel Shilla, Gert Everaert, Farhan R. Khan, and Yvonne Shashoua
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Polyethylene ,Microplastics ,Aquatic Science ,Oceanography ,Pollution ,Kenya ,Plastics ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Microplastics (MPs) were collected at six locations along Kenya's marine nearshore surface waters using a 300 μm mesh-size manta net. The samples were washed over a 125-μm mesh size sieve No.120 into a glass jar and preserved in 70% ethanol. MPs were sorted, counted visually under a dissecting microscope then identified using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. A total of 1473 particles with an overall mean concentration of 0.58 ± 1.29 MPs m
- Published
- 2021
37. Predicting the potential for zoonotic transmission and host associations for novel viruses
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Pranav Pandit, Simon Anthony, Tracey Goldstein, Kevin Olival, Megan Doyle, Nicole Gardner, Brian Bird, Woutrina Smith, David Wolking, Kristen Gilardi, Corina Monagin, Terra Kelly, Marcela Uhart, Jonathan Epstein, Catherine Machalaba, Melinda Rostal, Patrick Dawson, Emily Hagan, Ava Sullivan, Hongying Li, Aleksei Chmura, Alice Latinne, Christian Lange, Tammie O'Rourke, Sarah Olson, Lucy Keatts, A. Patricia Mendoza, Alberto Perez, Catia Dejuste de Paula, Dawn Zimmerman, Marc Valitutto, Matthew LeBreton, David McIver, Ariful Islam, Veasna Duong, Moctar Mouiche, Zheng-Li Shi, Prime Mulembakani, Charles Kumakamba, Mohamed Ali, Nigatu Kebede, Ubald Tamoufe, Samuel Bel-Nono, Alpha Camara, Joko Pamungkas, Julien Kalpy Coulibaly, Ehab Abu-Basha, Joseph Kamau, Soubanh Silithammavong, James Desmond, Tom Hughes, Enkhtuvshin Shiilegdamba, Ohnmar Aung, Dibesh Karmacharya, Julius Nziza, Daouda Ndiaye, Aiah Gbakima, Zikankuba Sijali, Supaporn Wacharapluesadee, Erika Alandia Robles, Benard Ssebide, Gerardo Suzán, Luis Aguirre, Monica Solorio, Tapan Dhole, Peta Hitchens, Damien Joly, Karen Saylors, Amanda Fine, Suzan Murray, William Karesh, Peter Daszak, Jonna Mazet, PREDICT Consortium, and Christine Johnson
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viruses ,Zoonoses ,Africa ,Viruses ,Medicine (miscellaneous) ,Animals ,Humans ,Animals, Wild ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology ,Host Specificity - Abstract
Host-virus associations have co-evolved under ecological and evolutionary selection pressures that shape cross-species transmission and spillover to humans. Observed virus-host associations provide relevant context for newly discovered wildlife viruses to assess knowledge gaps in host range and estimate pathways for potential human infection. Using models to predict virus-host networks, we predicted the likelihood of humans as host for 513 newly discovered viruses detected by large scale wildlife surveillance at high-risk animal-human interfaces in Africa, Asia, and Latin America. Predictions indicated that novel coronaviruses are likely to infect a greater number of host species than viruses from other families. Our models further characterize novel viruses through prioritization scores and directly inform surveillance targets to identify host ranges for newly discovered viruses.
- Published
- 2021
38. Ownership structure, bank stability and the financial performance of commercial banks in South Sudan
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Elizabeth Kalunda Nthambi, Joseph Kamau, and Bak Barnaba Chol
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media_common.quotation_subject ,Population ,Accounting ,Financial system ,Audit ,private-local ownership ,lcsh:Economic history and conditions ,Sudan ,lcsh:Social Sciences ,Ownership Structure ,joint venture ,0502 economics and business ,Statistical analysis ,Overall performance ,050207 economics ,education ,Financial Performance ,Statistical hypothesis testing ,media_common ,Government ,education.field_of_study ,050208 finance ,Financial performance ,foreign ownership ,Foreign ownership ,business.industry ,05 social sciences ,lcsh:Economic theory. Demography ,Regression analysis ,Independence ,lcsh:HB1-3840 ,lcsh:H ,Central bank ,public ownership ,lcsh:HC10-1085 ,Business - Abstract
Since independence in 2011, the Republic of South Sudan has witnessed growth in the financial systems and the overall economy. This has led to growth in the number of financial institutions in the country. There is however minimal research on their overall performance. Hence the current research sought to determine the effect of ownership structure, bank stability and the financial performance of commercial banks in South Sudan. The population for the study was all the 29 commercial banks in South Sudan. Secondary data was collected for the period 2012-2017 from audited annual financial reports of individual banks and the Central Bank of South Sudan reports while primary data was collected by the use of a semi-structured questionnaire. Collected data was edited, sorted and coded into SPSS 23 for subsequent data analysis using SPSS 23 statistical analysis tool. This research utilized both descriptive and inferential statistical methods in the analysis. Statistical tests to be utilized in the study included t-tests, f-test, regression models and ANOVA models. Findings of the research were presented using frequencies, percentages, means, standard deviation, correlation coefficients, charts, tables, and other statistical measures. Results of the study indicated there was a statistically significant moderating effect of ownership structure on the financial performance of commercial banks in South Sudan. This study recommends that the government should adopt better measures to safeguard public-owned commercial banks to improve their efficiency and performance.
- Published
- 2019
39. THE INFLUENCE OF INFORMATION SYSTEMS ON M-COMMERCE PERFORMANCE IN KENYA’S COMMERCIAL BANKS
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Joseph Kamau Ngugi, Doreen Muriu, and Zachary Mosoti
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Research design ,0209 industrial biotechnology ,021103 operations research ,Data collection ,Descriptive statistics ,business.industry ,Process (engineering) ,Mobile commerce ,0211 other engineering and technologies ,02 engineering and technology ,Unit of analysis ,Strategy implementation ,020901 industrial engineering & automation ,Information system ,Marketing ,business - Abstract
Purpose: The purpose of the study was to determine the influence of information systems strategy implementation on Mobile-Commerce (M-Commerce) performance in Kenya’s commercial banks. Methodology: The study adopted a positivism research philosophy and explanatory research design as appropriate methodology for the study. The units of analysis for the study comprised of five managers from different management levels and departments from each of the 40 commercial banks in Kenya. Questionnaires were used for data collection. The collected data was analyzed using inferential and descriptive statistics.Findings The study findings indicated that there was positive and statistically significant relationship between information systems and m-commerce performance. The path coefficient was positive and significant at 0.05 level, t-value of 2.031, p-value =0.043. Unique Contribution to theory, practice and policy: The study informs commercial banks in Kenya, Central Bank of Kenya, Communication Authorities and the scholars on the opportunities in the development of appropriate strategy implementation process and the risk of not adopting the right strategy.
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- 2019
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40. Socioeconomic status effects on health vary between rural and urban Turkana
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Benjamin Muhoya, Charles Waigwa, Dino J. Martins, Julie Peng, Joseph Kamau, Amanda J. Lea, Michael Gurven, Varada Abhyankar, Lucas P. Henry, Julien F. Ayroles, and Francis Lotukoi
- Subjects
education.field_of_study ,early life adversity ,Operationalization ,Reproductive success ,Offspring ,Health, Toxicology and Mutagenesis ,social gradients in health ,Population ,Pastoralism ,AcademicSubjects/SCI01130 ,Medicine (miscellaneous) ,Context (language use) ,Turkana ,socioeconomic status ,Editor's Choice ,AcademicSubjects/MED00860 ,Social determinants of health ,Original Research Article ,Psychology ,education ,Socioeconomic status ,Ecology, Evolution, Behavior and Systematics ,pastoralism ,Demography - Abstract
Background and objectives Understanding the social determinants of health is a major goal in evolutionary biology and human health research. Low socioeconomic status (often operationalized as absolute material wealth) is consistently associated with chronic stress, poor health and premature death in high-income countries. However, the degree to which wealth gradients in health are universal—or are instead made even steeper under contemporary, post-industrial conditions—remains poorly understood. Methodology We quantified absolute material wealth and several health outcomes among a population of traditional pastoralists, the Turkana of northwest Kenya, who are currently transitioning toward a more urban, market-integrated lifestyle. We assessed whether wealth associations with health differed in subsistence-level versus urban contexts. We also explored the causes and consequences of wealth-health associations by measuring serum cortisol, potential sociobehavioral mediators in early life and adulthood, and adult reproductive success (number of surviving offspring). Results Higher socioeconomic status and greater material wealth predicts better self-reported health and more offspring in traditional pastoralist Turkana, but worse cardiometabolic health and fewer offspring in urban Turkana. We do not find robust evidence for either direct biological mediators (cortisol) or indirect sociobehavioral mediators (e.g. adult diet or health behaviors, early life experiences) of wealth–health relationships in either context. Conclusions and implications While social gradients in health are well-established in humans and animals across a variety of socioecological contexts, we show that the relationship between wealth and health can vary within a single population. Our findings emphasize that changes in economic and societal circumstances may directly alter how, why and under what conditions socioeconomic status predicts health. Lay Summary High socioeconomic status predicts better health and more offspring in traditional Turkana pastoralists, but worse health and fewer offspring in individuals of the same group living in urban areas. Together, our study shows that under different economic and societal circumstances, wealth effects on health may manifest in very different ways.
- Published
- 2021
41. An ethnobotanical study of medicinal plants commonly traded in Kajiado, Narok and Nairobi counties, Kenya
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Ann Mwaura, Joseph Kamau, and Omwoyo Ombori
- Subjects
Prunus africana ,Osyris lanceolata ,Geography ,Traditional medicine ,biology ,Ethnobotany ,Plant species ,food and beverages ,Baseline data ,biology.organism_classification ,Medicinal plants - Abstract
Over eighty percent of the world population depend on traditional medicine for their basic health care needs. A study was carried out in three counties in Kenya (Kajiado, Narok and Nairobi) to document the common plant species traded as medicinal or herbal remedies. Structured interviews and questionnaire were administered to herbalists with prior informed consent, who were willing to disclose information on the source, plant type and parts of the herbal medicine they were selling and ailments treated. The folk or common names were recorded and later translated to scientific names using para-taxonomists and previous published data. Majority of the herbalists interviewed were between the ages of 40-59 years and comprised of mostly women (54%). The investigations revealed that eighty-six (86) plant species were traded as medicinal plants out of which 51% were commonly traded across the three counties. The study further revealed that the most traded plant parts were stem, bark and roots which could pose a threat to conservation of the species due to complete or partial destruction of the trees during harvesting. Aloe species, Prunus africana and Osyris lanceolata were highly traded an indication of their preference by local inhabitants to treat particular ailments. The generated list of medicinal plants species will form baseline data that could be used to generate a comprehensive list of all plant species traded as herbal medicine in Kenya. The commonly traded plants can also be included in pharmacological studies which may lead to development of new and potential drugs.
- Published
- 2020
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42. Evaluation of staA, viaB and sopE genes in Salmonella detection using conventional polymerase chain reaction (PCR)
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Pauline Kwamboka Getanda, Joseph Kamau, P.W. Kinyanjui, Francis Kariuki, Gerald Juma, and Atunga Nyachieo
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Serotype ,Salmonella ,Pcr cloning ,Biology ,biology.organism_classification ,medicine.disease ,medicine.disease_cause ,Typhoid fever ,Microbiology ,law.invention ,Salmonella enterica ,law ,medicine ,Gene ,Bacteria ,Polymerase chain reaction - Abstract
Typhoid fever is caused by the bacteria Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) and remains a significant health problem in many developing countries. The lack of adequate diagnostic capabilities in these poor resource settings have contributed greatly in making typhoid fever endemic in these regions. Reliable and inexpensive diagnostic tests are needed to improve the management of this disease burden. This study evaluated the ability of staA, viaB and sopE genes to detect Salmonella spp. Conventional polymerase chain reaction (PCR) amplification of staA, viaB and sopE genes of Salmonella was used to detect and differentiate between the three most prevalent Salmonella spp. in Kenya (S. Typhi, S. Typhimurium and S. Enteritidis). The staA primers (StaA-Forward / StaA-Reverse) and viaB primers (vi-Forward / vi-Reverse) were found to be specific only for the different strains of S. Typhi, producing PCR products of 585 bp and 540 bp respectively. No amplification was observed with S. Typhimurium, S. Enteritidis, E. coli and S. boydii bacterial strains. The sopE primers (SopE-Forward / SopE-Reverse) was demonstrated to be specific for all Salmonella spp. producing a 465 bp PCR product with no amplification observed with the E. coli and S. boydii bacterial strains. Conventional PCR using these staA and viaB primers for detection of S. Typhi shows great potential for diagnosis of typhoid fever however, further studies need to be carried out with actual food samples and human samples (blood, stool or saliva) to determine the effectiveness of this method in the detection of common Salmonella spp. in Kenya.Author summaryTyphoid fever is a severe disease caused by the bacteria Salmonella Typhi (S. Typhi) and is a significant health problem in many developing countries. The lack of adequate diagnostic capabilities in poor resource settings common in most public health facilities in Kenya and Africa in general, hinder prompt diagnosis of typhoid fever. Currently, the available diagnostic tests are often expensive and more so not readily available in most resource poor endemic areas. This has often led to misdiagnosis of the disease, thereby delaying appropriate treatment and making typhoid fever widespread in most resource poor areas. This study examines the ability of three different genes to detect and differentiate between the three most prevalent Salmonella strains in Kenya using a readily available and widely used genetic test known as conventional polymerase chain reaction (PCR). This research found that staA and viaB genes were specific only for S. Typhi, while the sopE gene was specific for all Salmonella strains. Consequently, conventional PCR using these staA and viaB genes for detection of S. Typhi shows great potential to be used as a readily available diagnostic tool to detect the presence of the S. Typhi organism in individuals or foods sample in Kenya.
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- 2020
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43. Shelf‐break upwelling and productivity over the North Kenya Banks: The importance of large‐scale ocean dynamics
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Francesco Nencioli, Stuart C. Painter, Joseph Kamau, Martin R. Palmer, Michael J. Roberts, Juliane Wihsgott, Fatma Jebri, Zoe Jacobs, Ekaterina Popova, Meric Srokosz, and Dionysios E. Raitsos
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Ocean dynamics ,Indian ocean ,Geophysics ,Oceanography ,Scale (ratio) ,Space and Planetary Science ,Geochemistry and Petrology ,Earth and Planetary Sciences (miscellaneous) ,Environmental science ,Upwelling ,Shelf break ,Productivity - Abstract
The North Kenya Banks (NKBs) have recently emerged as a new frontier for food security and could become an economically important fishery for Kenya with improved resources providing better accessibility. Little research has been done on the mechanisms supporting high fish productivity over the NKBs with information on annual and interannual environmental variability lacking. Here we use a high‐resolution, global, biogeochemical ocean model with remote sensing observations to demonstrate that the ocean circulation exerts an important control on the productivity over the NKBs. During the Northeast Monsoon, which occurs from December to February, upwelling occurs along the Kenyan coast, which is topographically enhanced over the NKBs. Additionally, enhanced upwelling events, associated with widespread cool temperatures, elevated chlorophyll, nutrients, primary production, and phytoplankton biomass, can occur over this region. Eight such modeled events, characterized by primary production exceeding 1.3 g C/m−2/day, were found to occur during January or February from 1993–2015. Even though the upwelling is always rooted to the NKBs, the position, spatial extent, and intensity of the upwelling exhibit considerable interannual variability. The confluence zone between the Somali Current and East African Coastal Current (referred to as the Somali‐Zanzibar Confluence Zone) forms during the Northeast Monsoon and is highly variable. We present evidence that when the Somali‐Zanzibar Confluence Zone is positioned further south, it acts to enhance shelf‐edge upwelling and productivity over the NKBs. These findings provide the first indication of the environmental controls that need to be considered when developing plans for the sustainable exploitation of the NKB fishery.
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- 2020
44. Endemicity of Yaws and Seroprevalence of Treponema pallidum Antibodies in Nonhuman Primates, Kenya
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Emily H. Hardgrove, Daniel Chai, Peris Ambala, Samson Kamawe Mutura, Joseph Kamau, Sascha Knauf, Dawn Zimmerman, Joseph G. Maina, Velma Kivali, Fatima Hussein, Michael E. von Fricken, and Andrea Surmat
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Primates ,Microbiology (medical) ,genetic structures ,Epidemiology ,nonhuman primates ,030231 tropical medicine ,lcsh:Medicine ,Papio anubis ,primate ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,0302 clinical medicine ,Seroepidemiologic Studies ,biology.animal ,parasitic diseases ,Research Letter ,Prevalence ,Animals ,antibodies ,Seroprevalence ,Public Health Surveillance ,lcsh:RC109-216 ,Primate ,Treponema pallidum ,030212 general & internal medicine ,Vervet monkey ,bacteria ,Treponema ,vervet monkey ,baboon ,biology ,Monkey Diseases ,lcsh:R ,yaws ,biology.organism_classification ,Antibodies, Bacterial ,Kenya ,Virology ,Endemicity of Yaws and Treponema pallidum Antibodies in Nonhuman Primates, Kenya ,zoonoses ,Infectious Diseases ,Laikipia County ,biology.protein ,Epidemiologic data ,Antibody ,Baboon - Abstract
Human yaws has historically been endemic to Kenya, but current epidemiologic data are lacking. We report seroprevalence for Treponema pallidum antibodies in olive baboons (Papio anubis) and vervet monkeys (Chlorocebus pygerythrus) in Laikipia County, Kenya. Our results suggest endemicity of the yaws bacterium in monkeys, posing a possible zoonotic threat to humans. US Agency for International Development Emerging Pandemic Threats PREDICT ProjectUnited States Agency for International Development (USAID) [GHN-A-OO-09-00010-00]; German Research FoundationGerman Research Foundation (DFG) [DFG KN 1097/3-1] This study was supported by the US Agency for International Development Emerging Pandemic Threats PREDICT Project (cooperative agreement mo. GHN-A-OO-09-00010-00) and the German Research Foundation (grant DFG KN 1097/3-1 to S.K.).
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- 2019
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45. Population genetics of Trypanosoma evansi from camel in the Sudan.
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Bashir Salim, Thierry de Meeûs, Mohammed A Bakheit, Joseph Kamau, Ichiro Nakamura, and Chihiro Sugimoto
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Arctic medicine. Tropical medicine ,RC955-962 ,Public aspects of medicine ,RA1-1270 - Abstract
Genetic variation of microsatellite loci is a widely used method for the analysis of population genetic structure of microorganisms. We have investigated genetic variation at 15 microsatellite loci of T. evansi isolated from camels in Sudan and Kenya to evaluate the genetic information partitioned within and between individuals and between sites. We detected a strong signal of isolation by distance across the area sampled. The results also indicate that either, and as expected, T. evansi is purely clonal and structured in small units at very local scales and that there are numerous allelic dropouts in the data, or that this species often sexually recombines without the need of the "normal" definitive host, the tsetse fly or as the recurrent immigration from sexually recombined T. brucei brucei. Though the first hypothesis is the most likely, discriminating between these two incompatible hypotheses will require further studies at much localized scales.
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- 2011
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46. Productivity driven by Tana river discharge is spatially limited in Kenyan coastal waters
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Francesco Nencioli, Stuart C. Painter, Stephen Kelly, Amon Kimeli, Amina Makori, Philip Kibet Langat, Stephen Carpenter, Dionysios E. Raitsos, Damaris Mutia, Fatma Jebri, Ekaterina Popova, Michael J. Roberts, Joseph Kamau, and Zoe Jacobs
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0106 biological sciences ,Wet season ,Kenya ,010504 meteorology & atmospheric sciences ,Discharge ,010604 marine biology & hydrobiology ,Management, Monitoring, Policy and Law ,Aquatic Science ,Oceanography ,Livelihood ,01 natural sciences ,Fishery ,Nutrient ,Productivity (ecology) ,Habitat ,Environmental science ,Bay ,0105 earth and related environmental sciences - Abstract
The Tana River is the longest river system in Kenya (~1000 km) and contributes ~ 50% of the total river discharge to Kenyan coastal waters. The river discharges significant amounts of nutrients and sediments, reaching ~24,000 tons per day during the rainy season (March–April), into Ungwana Bay (North Kenya Banks). The bay is an important habitat for high-value Panaeid prawn species which sustain important small-scale fisheries, semi-industrial bottom trawl prawn fisheries, and is the livelihood mainstay in the surrounding counties. In this study we analysed >20 years of satellite-derived chlorophyll-a observations (Chl-a, an index of phytoplankton biomass), along with in situ river discharge and rainfall data, to investigate if the Tana River discharge is a major driver of local phytoplankton biomass in Ungwana Bay and for the neighbouring Kenyan shelf. We find that during the rainy inter-monsoon (March–April), a significant positive relationship (r = 0.63, p < 0.0001) exists between river discharge and phytoplankton biomass. There is a clear time-lag between rainfall, river discharge (1-month lag) and local chlorophyll biomass (2-months lag after discharge). Unlike offshore waters which exhibit bi-annual chl-a peaks (0.22 mg m−3 in February, and 0.223 mg m−3 in August/September), Ungwana Bay displays a single peak per annum in July (2.51 mg m−3), with indications that river discharge sustains phytoplankton biomass for several months. Satellite-derived observations and Lagrangian tracking simulations indicate that higher Chl-a concentrations remain locally within the bay, rather than influencing the broader open waters of the North Kenya Banks that are mainly impacted by the wider oceanic circulation.
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- 2021
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47. Analysis of Combining Ability for Early Maturity and Yield in Rice (Genus: Oryza) at the Kenyan Coast
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Dianga, Al-Imran, primary, Joseph, Kamau W., additional, and Musila, Ruth N., additional
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- 2020
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48. Development of neck surface electromyography gaming control interface for application in tetraplegic patients’ entertainment
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Muguro, Joseph Kamau, primary, Sasaki, Minoru, additional, Matsushita, Kojiro, additional, Njeri, Waweru, additional, Laksono, Pringgo Widyo, additional, and Suhaimi, Muhammad Syaiful Amri bin, additional
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- 2020
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49. Trend analysis and fatality causes in Kenyan roads: A review of road traffic accident data between 2015 and 2020
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Muguro, Joseph Kamau, primary, Sasaki, Minoru, additional, Matsushita, Kojiro, additional, and Njeri, Waweru, additional
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- 2020
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50. EFFECT OF COMPREHENSIVE STRATEGIC DECISION-MAKING AND LONG-TERM ORIENTATION ON THE ORGANIZATIONAL PERFORMANCE OF DAIRY CO-OPERATIVES IN KENYA
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Joseph Kamau Ngugi, George O. K’Aol, and Joshua Wathanga
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education.field_of_study ,Descriptive statistics ,Product innovation ,Corporate governance ,Population ,Statistics ,Market orientation ,Revenue ,Business ,education ,Moderation ,Organizational performance - Abstract
Purpose: The purpose of this study was to investigate the effect of comprehensive strategic decision-making and long-term orientation on the organizational performance of dairy co-operatives in Kenya. Methodology: The study adopted the positivist research philosophy and descriptive correlational research design. The population of the study consisted of 198 executive directors/managers of active dairy co-operatives in eight counties in the Mt. Kenya region. A sample size of 184 was drawn using stratified random sampling, and data was collected using self-administered questionnaires. The data was then analyzed using descriptive statistics of frequency, mean, and standard deviation. Additionally, inferential data analysis methods of Pearson’s correlation, ANOVA, and multiple linear regression were used to test the hypotheses. Results : The multiple linear regression results indicated that long-term orientation significantly predicted revenue per customer, b = 9.85, t (141) = 3.35, p
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- 2017
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