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The augmented demand for sustainable nanocomposites has paved the way to explore naturally derived materials. Nanocellulose, with its bountiful sources and inherent properties, ranks top in the list of biofillers with a perspective of reducing the carbon footprint. A systematic study is required to understand the reinforcing effect of various types of nanocellulose. In the present work, we selected three types of nanocellulose, i.e., cellulose nanocrystal (CNC), cellulose nanofiber (CNF) and microfibrillated cellulose (MFC), to investigate the effect of geometrical structure on the properties of unvulcanized natural rubber (NR). Incorporating these fillers improved the tensile strength and modulus of natural rubber films significantly through reinforcement via filler network structure. The reinforcing effect of CNF was found to be higher compared to CNC and MFC, where an increase of 3.85 MPa in tensile strength from the neat sample was obtained. More uniform dispersion was evident through transmission electron microscopy, atomic force microscopy and Raman imaging for CNF in the rubber matrix. The structural properties were determined using Raman spectra and X-ray diffraction. The rheological studies revealed a good interaction between filler and NR. The work presented comprehensively compares different types of nanocellulose as reinforcing filler in NR matrix, which will help the researchers select an ideal type for their specific application and, thus, the proper usage of renewable resources, leading to sustainability and a circular economy.

Crohn’s disease (CD) is characterized as a chronic, relapsing, and progressive disorder with a complex etiology involving interactions between host, microbiome, and the external environment. Genome wide association studies (GWAS) suggest several genetic variations in the diseased individuals but that explains only a small proportion of susceptibility to disease conditions. This indicates the possible role of epigenome which links environmental factors to the genetic variation in the disease etiology. The current study is focused on the DNA methylome evolution with disease progression. We performed Reduced Representation Bisulfite Sequencing (RRBS) to analyze differential DNA methylation in the diseased and healthy mucosal tissues of 2 different groups of CD patients: non-surgical and surgical, categorized based on the severity of disease and standard of care needed. Patients in both groups have unique DNA methylation signature compared to the healthy tissue. After removing single nucleotide polymorphisms (SNPs), 1,671 differentially methylated loci were found in the non-surgical and 3,334 in the surgical group of which only 206 were found overlapping in both groups. Furthermore, differential DNA methylation was noted in some of the GWAS associated genes implicated in CD. Also, functional enrichment analysis showed high representation of several key pathways where differential methylations were observed, and these can be implicated in CD pathogenesis. We identified specific DNA methylation patterns in the mucosal DNA of surgical and non-surgical CD patients which indicates evolution of the methylome as the disease progresses from initial to the advance stage. These unique patterns can be used as DNA methylation signatures to identify different stages of the disease.

Heritage issues have increased significantly in recent years. However, they tend to remain in the cultural sphere and are often resistant to scientific analyses. If we have to deal with the contradictory matter of sustainability in design for ancient buildings, such hindrances appear frequently. A crucial aspect in Architecture has always been its capacity to dispose internal spaces and apertures in a manner that enhances the balance of light and thus provides attuned perception and well-being. Poor performance in that respect raised objections against the prestige of admirable works and famed artists. If we reject the absurd idea of accurately reproducing identical buildings in the same place repeatedly, how are we supposed to benefit from the said knowledge without the help of any objective design tools? It is easy to agree that at least we would need some scientific support to transmit such proper effects. Aware of the former notions, authors have developed a novel simulation software called DianaX, which is based on mathematical models and equations produced and expanded by Joseph Cabeza-Laïnez, from roughly 1990 to 2018. This non-commercial software deals with radiative exchanges in all kinds of surfaces (for instance domes, vaults, cylinders, hyperboloids and curves in general). It also includes direct sun in the simulations unlike most programs. Therefore, it is ideally suited for the analysis of heritage architecture and especially that which identifies with the Renaissance, baroque and neoclassical epochs. The case of temples from the baroque period resumes the conflict expressed in the first paragraph and the Jesuit Church of Saint Louis (1699-1731) is one of the most relevant examples of efficient illumination found in Mediterranean latitudes, having been recently restored. In this article, we would like to discuss the subtle and interesting implications of employing our simulation software for lighting in such a complex baroque temple. The methodology would be to identify the main energy sources within the church in order to construct a suitable model for simulation. Subsequently we apply the said software DianaX to such model and establish the most significant results trying to compare them with available on-site measurements. Finally, a strategy to enhance day-lighting and supplement it with other light sources in the church is proposed.

We present a scientific discussion about Chinese historical architecture and cultural paradigms in order to analyze the formation of building patterns objectively connected to environmental features. In this regard, we will demonstrate the process of standardization from architectural modules related in different levels of composition around "voids", onto cosmological urban tissues in harmony with nature. The conclusions show that we can only understand Chinese architectural patterns in relation to Dào or nature, and in turn, they possess profound social and environmental values from which we receive useful lessons to advance towards sustainability in architecture and urban planning. The authors believe that it is critical for China and the world to find a new approach to the building construction industry with an ecological and philosophical background recognizable as "Chinese" and based in its own past. In order to support the information provided in the first part of the article, the authors have conducted an environmental analysis of the traditional Chinese urban layout whose results greatly confirm the initial hypotheses, i.e. the historical fashion of constructing neighborhoods improves conditions of the town in terms of comfort and is able to save energy, thus reducing pernicious change effects.

Concepts to ameliorate the continued mismatch between demand for liver allografts and supply include the acceptance of allografts that meet extended donor criteria (ECD). ECD grafts are generally associated with an increased rate of complications such as early allograft dysfunction (EAD). The costs of liver transplantation for the health care system with respect to specific risk factors remain unclear and are subject to change. We analyzed 317 liver transplant recipients from 2013 to 2018 for outcome after liver transplantation and hospital costs in a German transplant center. In our study period, 1‐year survival after transplantation was 80.1% (95% confidence interval: 75.8%‐84.6%) and median hospital stay was 33 days (interquartile rage: 24), with mean hospital costs of €115,924 (SD €113,347). There was a positive correlation between costs and laboratory Model for End‐Stage Liver Disease score (rs = 0.48, P

Perfluorooctanoic acid (PFOA), a ubiquitous environmental toxicant from the Per- and polyfluoroalkyl substances (PFAS) family has been implicated in toxicity of various organs. Several epidemiological studies have linked PFOA to different lung injuries and diseased conditions. However, the implication of PFOA in affecting epigenetic regulators and SARS-CoV-2 infection pathways in the lung are unknown. The present work explores the accumulation of PFOA in lungs and changes in mRNA expression of DNA methylation regulator genes DNA methyltransferases (Dnmts) and ten-eleven translocation (Tets) along with the membrane proteins angiotensin converting enzyme 2 (Ace2) and transmembrane Serine Protease 2 (Tmprss2) genes involved in the SARS-CoV-2 virus infection. CD1 mice were orally exposed to 5 and 20 mg/kg/day PFOA for 10 days and the lung tissues were analyzed using LCMS, qPCR, and pyrosequencing techniques. PFOA was shown to accumulate in the lung tissues and increase in a dose-dependent manner. Dnmts and Tets were significantly downregulated upon at least one of the PFOA dosing concentration, whereas Ace2 and Tmprss2 show significant increase in their expression level. Further, CpG islands in the promotor region of Tmprss2 exhibited significant hypomethylation in PFOA treated groups, which supports its increased gene expression level. Current study reveals the implication of PFOA induced DNA methylation changes in lungs and their possible role in upregulation of Ace2 and Tmprss2. It is possible that increased expression of these membrane receptors due to PFOA exposure can lead to higher susceptibility of SARS-CoV-2 infections.

Abstract Background Absence from school can lead to lower educational achievement and poor health. Little is known about school absence in primary education. This study’s first aim was to examine the prevalence of school absence in primary schools and differing types of absence, including sickness absence. The second aim was to determine which pupil characteristics and types of absence were associated with extensive sickness absence. Methods The school absence registries for the school year 2015–2016 were analysed retrospectively in eight mainstream primary schools with 2216 pupils, and six schools for special primary education with 1000 pupils in the West-Brabant region of the Netherlands. Descriptive analyses, χ2-tests, Mann-Whitney U tests and logistic regression analyses were performed. Results The one-year prevalence of school absence was 85% in mainstream primary schools and 79% in special schools. Sickness absence was the most prevalent type of absence, occurring in 75 and 71% of pupils, respectively The prevalence of extensive sickness absence was 13 and 23%, respectively. In mainstream schools, extensive sickness absence was associated with a young age, low parental educational level, more doctor’s visits and unauthorised absence, and in special schools with more doctor’s visits, other authorised absence, tardiness and unauthorised absence. Conclusions The prevalence of extensive sickness absence was high, and as this was associated with other types of absence, these pupils missed even more days of school. Public health research, policy and practice should address sickness absence among primary school pupils, to prevent adverse effects on children’s development.

Alterations of the normal gut microbiota can cause various human health concerns. Environmental chemicals are one of the drivers of such disturbances. The aim of our study was to examine the effects of exposure to perfluoroalkyl and polyfluoroalkyl substances (PFAS)—specifically, perfluorooctane sulfonate (PFOS) and 2,3,3,3-tetrafluoro-2-(heptafluoropropoxy) propanoic acid (GenX)—on the microbiome of the small intestine and colon, as well as on liver metabolism. Male CD-1 mice were exposed to PFOS and GenX in different concentrations and compared to controls. GenX and PFOS were found to have different effects on the bacterial community in both the small intestine and colon based on 16S rRNA profiles. High GenX doses predominantly led to increases in the abundance of Clostridium sensu stricto, Alistipes, and Ruminococcus, while PFOS generally altered Lactobacillus, Limosilactobacillus, Parabacteroides, Staphylococcus, and Ligilactobacillus. These treatments were associated with alterations in several important microbial metabolic pathways in both the small intestine and colon. Untargeted LC-MS/MS metabolomic analysis of the liver, small intestine, and colon yielded a set of compounds significantly altered by PFOS and GenX. In the liver, these metabolites were associated with the important host metabolic pathways implicated in the synthesis of lipids, steroidogenesis, and in the metabolism of amino acids, nitrogen, and bile acids. Collectively, our results suggest that PFOS and GenX exposure can cause major perturbations in the gastrointestinal tract, aggravating microbiome toxicity, hepatotoxicity, and metabolic disorders.

Objectives Sellar pathologies are frequently found on imaging performed to investigate headache. However, both headache and incidental sellar lesions are common. Hence, this study prospectively examined headache prevalence, phenotype, and severity in patients with sellar pathologies and the impact of transsphenoidal surgery on headache. Methods Patients undergoing transsphenoidal resection of sellar lesions were consecutively recruited. At baseline, participants were defined as having headache or not and headache phenotype was characterized using validated questionnaires. Headache severity was assessed at baseline and 6 months postoperatively using the Headache Impact Test-6 (HIT-6) and Migraine Disability Assessment Score (MIDAS). Tumor characteristics were defined using radiological, histological, and endocrine factors. Primary outcomes included baseline headache prevalence and severity and headache severity change at 6 months postoperatively. Correlation between headache and radiological, histological, and endocrine characteristics was also of interest. Results Sixty participants (62% female, 47.1 ± 18.6 years) were recruited. Sixty-three percent possessed baseline headache. HIT-6 scores were higher in patients with primary headache risk factors, including younger age (R 2 = −0.417, p = 0.010), smoking history (63.31 ± 7.93 vs 54.44 ± 9.21, p = 0.0060), and family headache history (68.13 ± 7.01 vs 54.94 ± 9.11, p = 0.0030). Headaches were more common in patients with dural invasion (55.70 ± 12.14 vs 47.18 ± 10.15, p = 0.027) and sphenoid sinus invasion (58.87 ± 8.97 vs 51.29 ± 10.97, p = 0.007). Postoperative severity scores improved more with higher baseline headache severity (HIT-6: R 2 = −0.682, p < 0.001, MIDAS: R 2 = −0.880, p < 0.0010) and dural invasion (MIDAS: −53.00 ± 18.68 vs 12.00 ± 17.54, p = 0.0030). Conclusion Headaches in sellar disease are likely primary disorders triggered or exacerbated by sellar pathology. These may respond to surgery, particularly in patients with severe headache and dural invasion. [ABSTRACT FROM AUTHOR]

Nanomaterials have engaged response from the scientific world in recent decades due to their exceptional physical and chemical properties counter to their bulk. They have been widely used in a polymer matrix to improve mechanical, thermal, barrier, electronic and chemical properties. In rubber nanocomposites, nanofillers dispersion and the interfacial adhesion between polymer and fillers influences the composites factual properties. In the present work, a comparison of the hybrid effects of carbon black with two different nanofillers (graphene oxide and nanoclay) was studied. The 70/30 composition of chlorobutyl rubber/natural rubber elastomer blend was taken as per the blend composition optimized from our previous studies. The hybrid effects of graphene oxide and nanoclay in dispersing the nanofillers were studied mainly by analyzing nanocomposite barrier properties. The results confirm that the combined effect of carbon black with graphene oxide and nanoclay could create hybrid effects in decreasing the gas permeability. The prepared nanocomposites which partially replace the expensive chlorobutyl rubber can be used for tyre inner liner application. Additionally, the reduction in the amount of carbon black in the nanocomposite can be an added advantage of considering the environmental and economic factors.

Diverse problems of radiative transfer remain as yet unsolved due to the difficulties of the calculations involved, especially if the intervening shapes are geometrically complex. The main goal of our investigation in this domain is to convert the equations that were previously derived into a graphical interface based on the projected solid-angle principle. Such a procedure is now feasible by virtue of several widely diffused programs for Algorithms Aided Design (AAD). Accuracy and reliability of the process is controlled in the basic examples by means of subroutines from the analytical software DianaX, developed at an earlier stage by the authors, though mainly oriented to closed cuboidal or curved volumes. With this innovative approach, the often cumbersome calculation procedure of lighting, thermal or even acoustic energy exchange can be simplified and made available for the neophyte, with the undeniable advantage of reduced computer time.

Perfluorooctanoic acid (PFOA) has been implicated in various toxicities including neurotoxicity, genotoxicity, nephrotoxicity, epigenetic toxicity, immunotoxicity, reproductive toxicity, and hepatotoxicity. However, information on the accumulation of PFOA in the intestine and its toxic effects on intestinal epigenetics and tight junction (TJ) genes is sparse. CD1 mice were dosed with PFOA (1, 5, 10, or 20 mg/kg/day) for 10 days, and its accumulation and induced alterations in the expression of epigenetic and tight junction genes in the small intestine and colon were evaluated using LC–MS and qPCR techniques. PFOA reduced the expression levels of DNA methyltransferases (Dnmt1, Dnmt3a, Dnmt3b) primarily in the small intestine whereas, in the colon, a decrease was observed only at high concentrations. Moreover, ten-eleven translocation genes (Tet2 and Tet3) expression was dysregulated in the small intestine, whereas in the colon Tets remained unaffected. The tight junction genes Claudins (Cldn), Occludin (Ocln), and Tight Junction Protein (Tjp) were also heavily altered in the small intestine. TJs responded differently across the gut, in proportion to PFOA dosing. Our study reveals that PFOA triggers DNA methylation changes and alters the expression of genes essential for maintaining the physical barrier of intestine, with more profound effects in the small intestine compared to the colon.

While there is evidence for variations in prevalence rates of childhood wheeze and asthma between countries, longitudinal, individual-level data are needed to understand these differences. The aim of this study was to examine variations in prevalence rates of childhood asthma, wheeze and wheeze with asthma in Europe. We analysed datasets from 10 MeDALL (Mechanisms of the Development of ALLergy) cohorts in eight countries, representing 26 663 children, to calculate prevalence rates of wheeze and asthma by child age and wheeze with asthma at age 4 years. Harmonised variables included outcomes parent-reported wheeze and parent-reported doctor-diagnosed asthma, and covariates maternal education, parental smoking, pets, parental asthma, doctor-diagnosed allergic rhinitis, doctor-diagnosed eczema and wheeze severity. At age 4 years, asthma prevalence varied from 1.72% in Germany to 13.48% in England and the prevalence of wheeze varied from 9.82% in Greece to 55.37% in Spain. Adjusted estimates of the proportion of 4-year-old children with wheeze diagnosed with asthma remained highest in England (38.14%, 95% CI 31.38–44.90%) and lowest in Spain (15.94%, 95% CI 6.16–25.71%). The large differences in prevalence rates of asthma, wheeze and wheeze with asthma at age 4 years between European cohorts may indicate that childhood asthma is more readily diagnosed in some countries while going unrecognised elsewhere.