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The genetic diversity in the red clover USA core collection was measured by RAPD and isozyme markers,comparing different approaches of isozyme data analysis and comparing the results of RAPD with those of isozymes. A totalof 15 isozyme loci and 114 RAPD fragments were analyzed. Genetic diversity measured with RAPD and isozymes was high.The analyses of isozymes as binary data was highly correlated with the allele-frequency approach. There was no correlationbetween the distance matrices of different markers. Isozyme data grouped the accessions of red clover in four groups. Thevariation evidenced by AMOVA was higher among and within groups in the populations than at the intra-population level.Results showed the high diversity in the red clover core collection and indicated some populations that could be used inbreeding programs of the crop in Brazil.

Com o objetivo de descrever a variabilidade genética e química da planta medicinal chapéu de couro (Echinodorus grandiflorus Mich. e outras espécies) existente no Rio Grande do Sul, foram coletadas plantas em 16 diferentes localidades e transplantadas para uma área experimental na Universidade Federal de Pelotas, em Pelotas-RS. As plantas foram cultivadas em planossolo alagado no inverno e com irrigação suplementar quando houve umidade insuficiente no solo. A adubação das plantas foi calcáreo, cinza de casca de arroz e vermicomposto. As folhas e sementes foram colhidas na primavera e verão. Foi procedida a descrição dos ecotipos, através dos parâmetros fenológicos: início da brotação, vigor da brotação, florescimento, antese, maturação das sementes e, agronômicos: resistência ao frio e à insolação, competição com plantas daninhas e peso da matéria seca foliar, bem como uma análise dos compostos químicos majoritários nas folhas. As avaliações permitiram concluir que: 1- a espécie predominante no Rio Grande do Sul é a Echinodorus grandiflorus; 2 - os ecotipos de Cruz Alta e de São Gabriel são recomendados para cultivo como precocidade e produção de matéria seca; 3 - os ecotipos de Arroio Grande e Jaguarão são recomendados para cultivo pela composição química mais numerosa.The aim of this research work was to describe the genetic and chemical variability of the medicinal specie "chapéu de couro" (Echinodorus grandiflorus and other species), which is found in Rio Grande do Sul State, Brazil. The plants had been collected at sixteen different sites and planted again in an experimental area at the Federal University of Pelotas (UFPel), in the town of Pelotas-RS. The plants have grown in waterlands, by keeping them underwater during the winter and adding water when the soil was dry. The plants have been fed up with lime, ash of rice waste and vermcomposite. The leaves and the seeds have been collected during spring and summer. The phenology of the different material has been observed to proceed the botanical and physiological description of ecotypes, through field parameters and in the experimental area. The chemical composition of the major chemical substances in the leaves of both native and non native plants was also analyzed. The evaluation led to conclude that: 1- the vailing specie in Rio Grande do Sul State is Echinodorus grandiflorus; 2 - the ecotypes from Cruz Alta and São Gabriel are prefered for cropping due to their early and dry matter production; 3 - the ecotypes from Arroio Grande and Jaguarão are prefered for cropping due to your rich chemical composition.