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The luteinizing hormone receptor (LHR) is a glycoprotein member of the G protein-coupled receptors superfamily. It participates in corpus luteum formation and ovulation in females and acts in testosterone synthesis and spermatogenesis in males. In this study, we extracted RNA from sheep testicles and synthetized the cDNA to amplify the gene lhr-bed. This gene consists of 762 bp and encodes 273 amino acids of the extracellular domain of LHR. The lhr-bed was cloned into pJET1.2/blunt, then subcloned into pCOLD II, and finally, transformed in E. coli BL21 (DE3) cells. Because the induced rLHR-Bed protein was found in the insoluble fraction, we followed a modified purification protocol involving induction at 25 °C, subjection to denaturing conditions, and on-column refolding to increase solubility. We confirmed rLHR-Bed expression by means of Western blot and mass spectrometry analysis. It is currently known that the structure stem-loop 5′UTR on pCOLD II vector is stable at 15 °C. We predicted and obtained RNAfold stability at 25 °C. We successfully obtained the recombinant LHR extracellular domain, with protein yields of 0.2 mg/L, and purity levels of approximately 90%, by means of a single chromatographic purification step. The method described here may be used to obtain large quantities of rLHR-Bed in the future.

Currently agriculture has difficulty keeping up with the demand for food around the world, which has generated a boom in the development of sustainable alternatives for producing food and caring for the environment. Therefore, the present study aims to show a backyard system comprising 50 cm × 50 cm pinewood boxes where maize and peanut were tested under control and compost conditions. The experiments were carried out for nine months starting from compost production and the sowing of the crops, which were irrigated with temporary rain. The compost was produced by converting ~213 kg of organic residues into ~300 kg of mature compost. The fertilizer treatment consisted of two doses of compost (1 kg doses). The developing plants were compared between conditions in both crops. In addition, the nutritional values of the compost and compost and soil were evaluated. Interestingly, the correlation analyses of the morphological properties of the soils showed that the effects of the nutrients were positively associated with the morphology of the crops studied. Finally, the yield produced for maize was 9 kg/m2 and 6.6 kg/m2 and that for peanuts was 184 g/m2 and 73 g/m2 under compost and control conditions, respectively. We consider that the development of new alternatives for producing food in times of crisis or situations of limited resources is necessary for the development of humanity and the care of the environment.

The Yucatan Peninsula, located in southern Mexico, is a central honey-producing region with extraordinary biodiversity of melliferous plants. Approximately 900 plant species have been described as being a source of nectar and pollen for bees and other pollinators. They provide ecosystem services that help to keep plant biodiversity high and mitigate the effects of climate change. This study aimed to reveal the diversity of the pollen content in honey through a melissopalynological analysis of 22 honey samples collected in February–August 2021 from the north-central area of Campeche, Mexico. The extraction of pollen from the honey was carried out using standard methods for melissopalynological analysis. The honeys were classified by botanical origin to determine their floral sources and a diverse spectrum of 19 pollen types from 13 families was identified. Only eight were predominant: Milleria quinqueflora, Gymnopodium floribundum, Terminalia buceras, Amaranthus spinosus, Zea mays, Talisia floresii, Guazuma ulmifolia, and Croton icche. Our research shows the high quality of the honey analyzed and highlights the diversity and critical role of local melliferous flora and crops in beekeeping development in southern Mexico. The results in this study are useful for confirming the botanical origins of honey, generating information for designing nature conservation and agroecosystem management strategies, and increasing the knowledge of beekeepers in Campeche, Mexico.