1. Microwave assisted high performance liquid chromatography for the separation of triacylglycerols in vegetable oils using an evaporative light scattering detector
- Author
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M. S. Prats-Moya, José Luis Todolí-Torro, Silvia Carballo-Marrero, Salvador E. Maestre-Pérez, Universidad de Alicante. Departamento de Química Analítica, Nutrición y Bromatología, Análisis de Alimentos, Química Culinaria y Nutrición (AAQCN), and Análisis de Polímeros y Nanomateriales
- Subjects
Almonds ,food.ingredient ,Materials science ,Argan oil ,Trilinolein ,Food chemistry ,01 natural sciences ,High-performance liquid chromatography ,Analytical Chemistry ,0404 agricultural biotechnology ,food ,Chromatography detector ,Phase (matter) ,Plant Oils ,Scattering, Radiation ,Microwaves ,Chromatography, High Pressure Liquid ,Triglycerides ,Chromatography ,Tiger nuts ,Elution ,010401 analytical chemistry ,Temperature ,Water ,04 agricultural and veterinary sciences ,General Medicine ,040401 food science ,Nutrición y Bromatología ,0104 chemical sciences ,Blood chemistry ,Triacylglycerols ,Química Analítica ,MW-HPLC ,Vegetable oils ,Food Analysis ,Triolein ,Microwave ,Food Science - Abstract
Microwave (MW) radiation was applied to perform the separation of triacylglycerols (TGs) in oil samples. The novelty of the work lies in the application of MW radiation to assist the separation of several non-polar compounds employing a totally organic mobile phase. Once the influence of the evaporative light scattering detector (ELSD) variables on the sensitivity was optimized, the TGs separation was compared conditioning the column with either a conventional HPLC or a MW oven. Contrary to previous applications in which the mobile phase contained water, the improvement in sensitivity using MW was not as significant in comparison with conventional heating but it allowed a shortening in the retention times of several TGs in about 50% respect elution at room temperature. The method was finally applied for the quantification of most common TGs in almond, tiger nut, and argan oil. Authors wish to thank the Spanish Ministry of Science, Innovation and Universities for the financial support (Project Ref. PGC2018-100711-B-I00).
- Published
- 2019
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