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Os estudos de Wiliam Labov, da década de 1960, cuja centralidade é a heterogeneidade linguística, ensejaram vários trabalhos no intuito de desnudar e deslegitimar metalinguagens preconceituosas advindas da equivocada noção da homogeneidade linguística. Todavia, esse tipo de abordagem inoportuna sobre a língua ainda é recorrente e precisa ser combatida, sobretudo, nas escolas. Nessa perspectiva, em consonância com a proposta do Programa de Mestrado Profissional em Letras, intervimos em sala de aula objetivando a conscientização dos discentes sobre o reconhecimento do Preconceito Linguístico como forma de combatê-lo, propondo a produção de contos com enredos que tratassem do referido preconceito linguístico. Com nossa intervenção, os resultados obtidos foram satisfatórios, uma vez que foi apresentada e compreendida a problemática do preconceito linguístico por meio da produção dos contos dos discentes.

Como indivíduos dotados de linguagem, os seres humanos se comunicam a partir de gêneros discursivos. Partindo disso, tem-se como objetivo neste trabalho analisar como o gênero discursivo charge representa e (re)constrói, discursivamente, o evento da Comissão Parlamentar de Inquérito (CPI). Para isso, o presente texto apoia-se nos pressupostos da Análise de Discurso Crítica (FAIRCLOUGH, 2003), ao compartilhar da ideia de discurso como prática social. Na análise de duas charges que tratam do evento CPI da Covid-19, percebe-se que a (re)construção de uma dada situação sociodiscursiva se dá a partir da mobilização de recursos semânticos como a ironia e a metáfora, como modo de fomentar uma reflexão crítica acerca do negacionismo e do anticientificismo do discurso bolsonarista. Conclui-se que o uso de gêneros discursivos não se dá de modo aleatório, mas sim como ações que geram criticidade e mudanças na estrutura social.

The study aimed to evaluate the effect of powdered coconut water-based diluent (ACP-101c) associated with extra virgin coconut oil (CO) as an external cryoprotectant in the conservation of cryopreserved buck sperm. For cryopreservation, the ejaculates from four bucks were pooled and divided into three aliquots and diluted at 37°C for treatments T1 (ACP-101c + 2.5% egg yolk +7% glycerol), T2 (ACP-101c + 2.5% CO +7% glycerol), and T3 (ACP-101c + 5% CO +7% glycerol). Then, the samples were packaged and cooled at a rate of 1.07°C/min decrease. Upon reaching 4°C, the samples were stored in a refrigerator at 4°C for 30 minutes for stabilization. After this period, the straws were frozen in nitrogen vapor for 15 minutes and then immersed and stored in liquid nitrogen at -196°C. After thawing, the samples were evaluated for sperm kinetics, plasma membrane integrity, acrosomal integrity, membrane functionality, mitochondrial activity (MA), and sperm morphology. In this study, no statistically significant differences were observed between the three treatments regarding the kinetic parameters (

The quality of post-thawing goat sperm is critical to the success of artificial insemination protocols and may be influenced by extenders, cryoprotectants, and antioxidant substances. Therefore, the objective of this study was to evaluate the effects of the antioxidant anethole on goat sperm diluted in preservation medium based on powdered coconut water (ACP-101c) and frozen. For that, each ejaculate was submitted to the following treatments: ACP-101c (control); control plus supplementation with 30, 300, or 2000 µg/mL anethole. The samples were thawed and evaluated for morphology, kinetics, membrane integrity, and reactive oxygen species (ROS). The addition of anethole increased morphological abnormalities (P < 0.05), however, it did not affect sperm kinetics. Flow cytometry analysis showed that sperm cells cryopreserved with 300 µg/mL anethole had lower acrosome integrity than those cryopreserved in other treatments. Evaluation of oxidative stress revealed that cells stored in the presence of 2000 µg/mL anethole had small amounts of ROS when compared to those preserved in the control medium alone or supplemented with 300 µg/mL anethole (P < 0.05). After cryopreservation of sperm with 2000 µg/mL anethole, the highest percentage of viable sperm without ROS was observed (P < 0.05). In conclusion, despite reducing ROS levels, the supplementation of anethole in ACP-101c did not affect sperm kinetics or membrane integrity post-thawing, however, it did cause morphological damage to sperm. A qualidade do espermatozoide caprino pós-descongelação é crítica para o sucesso dos protocolos de inseminação artificial e pode ser influenciada por extensores, crioprotetores e substâncias antioxidantes. Portanto, o objetivo deste estudo foi avaliar os efeitos do antioxidante anetole sobre espermatozoides caprinos diluídos em meio de conservação à base de água de coco em pó (ACP-101c) e congelados. Para tanto, cada ejaculado foi submetido aos seguintes tratamentos: ACP-101c (controle); controle mais suplementação com 30, 300 ou 2000 ug / mL de anetole. As amostras foram descongeladas e avaliadas quanto à morfologia, cinética, integridade de membranas e espécies reativas de oxigênio. A adição de anetole aumentou as anormalidades morfológicas (P < 0,05), no entanto, não afetou a cinética dos espermatozoides. A análise da citometria de fluxo mostrou que as células de esperma criopreservadas com 300 ug / mL anethole tinham integridade acrosma menor do que aquelas criopreservadas em outros tratamentos. A avaliação do estresse oxidativo revelou que as células armazenadas na presença de 2000 ug / mL anethole apresentaram pequenas quantidades de ROS quando comparadas às preservadas em meio de controle isoladamente ou suplementadas com 300 ug / mL anethole (P < 0,05). Após a criopreservação de espermatozoides com 2000 ug / mL anethole, observou-se a maior porcentagem de espermatozoides viáveis sem ROS (P < 0,05). A população com espermatozoides viáveis sem ROS foi maior quando utilizado 2.000 ug / mL (P < 0,05). Em conclusão, apesar de reduzir os níveis de ROS, a suplementação de anetole em ACP-101c não afetou a cinética espermática e a integridade da membrana pós-descongelação, entretanto, causou danos morfológicos nos espermatozoides.

Effects were assessed of the dilutants TRIS and ACP - 101c® with the addition of different guinea fowl (Numida meleagris) egg yolk concentrations. Fifteen ejaculates were collected from five goats of the Anglo Nubian breed. The ejaculates were pooled and then divided into 12 groups, two control groups (GC1 TRIS, with 2.5% Gallus gallus domesticus hen egg yolk GOGD), (GC2 Control Group ACP - 101c®, with the addition of 2.5% Gallus gallus domesticus hen egg yolk GOGD) and ten experimental groups (EG), containing TRIS and ACP added with different concentrations of egg yolk from guinea hen (Numida meleagris) (TRIS 2,5% GONM; TRIS 5% GONM; TRIS 10% GONM; TRIS 15% GONM; TRIS 20% GONM; ACP® 2,5% GONM; ACP® 5% GONM; ACP® 10% GONM; ACP® 15% GONM; ACP® 20% GONM). Then cryopreservation was carried out and the samples stored in liquid nitrogen (-196 °C). After seven days, the samples were thawed and assessed for spermatic kinetics, immunofluorescence and sperm morphology. Analysis of GOMN by the CASA system showed that the various parameters were similar to those of GOGD (P>0.05). The membrane integrity, mitochondrial potential and the acrosome were not influenced by the treatment (P>0.05) nor by the dilutant used for cryopreservation (P>0.05). The spermatic morphology was also preserved by the different GOGD and GONM concentrations in the ACP® and TRIS dilutants, with no statistically significant differences (P

Testicular degeneration is highly prevalent in ruminants reared in a tropical climate in Brazil. It is therefore necessary to assess semen quality periodically in this animals. The objective of this work was to define standards images (thermograms) by using infrared thermography (TIV), associated with computerized seminal evaluation (CASA) to predict testicular alterations in a non-invasive way. For this, 10 goat males were selected, and testis insulated to evaluate the seminal quality and testicular superficial temperature recorded in four different moments: before, during and early and later after insulation. Semen collections and rectal temperatures (TR) and superficial testis (TS) by TIV were assessed for 60 days after insulation. Data were expressed in media and standard errors and analysis of variation (ANOVA) with comparison between the moments using Tukey test at 5% of probability. Changes in seminal quality followed the same standard of changes in TS, with no influence of the latter one on rectal temperature. The mean testicular temperature increased more than 2 degrees above the normal mean testis temperature, with significant increasing (p

 Na postura padrão, a coluna apresenta curvaturas normais e os ossos dos membros inferiores ficam em alinhamento. Quando o componente estrutural é alterado, o corpo humano modifica-se para desempenhar tal situação da melhor forma possí­vel, o que pode ocasionar em um desvio postural. O objetivo desta pesquisa é avaliar a postura e seus comprometimentos em discentes de um curso de Fisioterapia, traçando o perfil clí­nico socioeconômico, conhecendo os comprometimentos posturais dos participantes da pesquisa. Tratou-se de um estudo transversal, descritivo e quantitativo realizado entre os meses de agosto de 2015 a junho de 2016, no Centro Universitário Estácio do Ceará com 32 discentes do curso de Fisioterapia. Os dados foram coletados através de uma ficha de avaliação postural desenvolvida para o inquérito e analisada no programa Excel® 2013 e confrontados com a literatura vigente sobre o assunto. O gênero feminino foi predominante em 75% da amostra. Em relação ao estado civil dos participantes, 87,5% eram solteiros. A média de idade foi de 24,4 anos. Em relação ao Índice de Massa Corpórea, foi evidenciado que 28 participantes (87,5%) apresentaram um peso normal, de acordo com sua altura e peso. Para verificar a presença de escoliose, foi realizado o Teste de Adams. Evidenciou-se que 72% dos participantes apresentaram gibosidade, 12,50% apresentaram gibosidade lombar, 43,75% apresentaram gibosidade torácica e 15,63% apresentaram gibosidade toracolombar. A maioria dos estudantes apresentou escoliose. No entanto, estudos com uma amostra maior devem ser realizados, a fim de que resultados mais abrangentes possam ser obtidos.Palavras-chave: escoliose, postura, Fisioterapia.

Effects were assessed of the dilutants TRIS and ACP - 101c® with the addition of different guinea fowl (

Platelet-rich plasma (PRP) has been considered a promising therapeutic alternative, since platelets are rich in growth factors that are used in the Regenerative Medicine field. However, fresh PRP cannot be stored for long periods. This study aimed to develop a protocol for obtaining lyophilized canine PRP capable of maintaining viability after its reconstitution. For that purpose, canine PRP extraction and lyophilization protocols were initially tested. Subsequently, assays were carried out to quantify the growth factors VEGF and TGF-β, before and after the lyophilization process, gelation test and the three-dimensional gel structure analysis of the reconstituted lyophilized PRP by electron microscopy, as well as in vitro cell proliferation test in lyophilized PRP gel. Additionally, the immunogenicity test was performed, using allogeneic samples of lyophilized PRP. The results showed that the lyophilized PRP had adequate therapeutic concentrations of growth factors VEGF and TGF-β (9.1pg/mL and 6161.6pg/mL, respectively). The reconstituted PRP gel after lyophilization showed an in vitro durability of 10 days. Its electron microscopy structure was similar to that of fresh PRP. In the cell proliferation test, an intense division process was verified in mesenchymal stem cells (MSCs) through the three-dimensional mesh structure of the lyophilized PRP gel. The immunogenicity test showed no evidence of an immune reaction. The findings were promising, suggesting the possibility of having a lyophilized canine PRP that can be marketed. New in vivo and in vitro studies must be carried out for therapeutic confirmation.

O referido trabalho teve como objetivo testar blocos mineralizadores para a suplementação de caprinos na região de Pentecoste, Ceará. Foram utilizados bloquetes sólidos constituídos de frações proteicas, macro e micro minerais, adicionados de água de coco em pó e moléculas químicas ativas para controle das endoparasitoses e ectoparasitoses. No período de 60 (sessenta) dias de disponibilidade ad libitum dos blocos para os animais, os mesmos foram pesados e controlados quanto a análise de obterminacao de parasitoses sanguíneos, e exames sorológicos para o diagnóstico das helmintoses presentes durante a duração da suplementação. Após os 60 dias dos tratamentos, os animais mostraram um melhor ganho de peso, uma taxa menor de ovos por grama de fezes, além de uma menor incidência de eimeirose. A suplementação mineral e proteica conferiu aos animais uma melhor performance produtiva e sanitária, demonstrando que o emprego e adoção por parte de produtores de caprinos dessa inovação tecnológica, permitirá aos animais uma fonte nutricional voltada para a realidade do criatório de caprinos com uma relação custo/benefício favorável.

Background Cancer remains as one of the major causes of mortality worldwide. Recent advances in nanoparticles based therapy mark a new era on cancer treatment. Many groups have investigated biological/physical effects of nanoparticles on tumour cells and how these vary with physical parameters such as particle size, shape, concentration and distribution. Magnetic hyperthermia (MHT) can be an alternative or an add-value therapy with demonstrated effectiveness. MHT uses magnetic nanoparticles, which can be directly applied to the tumour, where, by applying an external ac magnetic field, will promote a localized temperature increment that can be controlled. Methods LiFe5O8 nanocrystallites were synthesized by a novel and eco-friendly sol-gel process, using powder coconut water (PCW) has a mediated reaction medium. The dried powders were heat-treated between 400 and 1000 °C, being analysed their structure, morphology and magnetic properties; cytotoxicity and magnetic hyperthermia assays were performed. Results The heat-treated LiFe5O8 powder led to crystallite sizes from 50 nm to 200 nm. Increasing the temperature of the heat treatment, secondary phases tend to disappear. The cytotoxicity results revealed a non-cytotoxicity property, for concentrations below 5 mg/mL. The sample HT at 1000 °C, revealed hysteresis and magnetic saturation of 73 emu.g−1 at 300 K and a specific absorption rate(SAR) of about 80 W/g. Conclusions It was possible to synthesize LiFe5O8 by a novel, eco-friendly and sustainable route. The nanoparticles have a high heat efficiency, especially the sample heat-treated at 1000 °C, with SAR values similar to Fe3O4, showing potential to be used in the treatment of cancer, by MHT.

Esse trabalho teve como objetivo avaliar os efeitos da refrigeracao e congelacao do semen caprino utilizando diluentes de origem vegetal. Para isso, foi utilizado 20 amostras de semen de cinco machos caprinos, em que foi avaliado imediatamente apos a coleta, diluidos em ACP-101 e extrato liofilizado de Aloe vera e controle Tris . O semen foi refrigerado a 4 °C e congelado a -196 °C. Ao termino da refrigeracao e apos descongelacao as amostras foram avaliadas quanto aos parâmetros cineticos de motilidade, velocidades e viabilidade. Os resultados das avaliacoes, demonstraram que o diluente ACP apresentou resultados superiores em comparacao aos tratamentos com Aloe vera . Portanto, a utilizacao do ACP-101 associado aos crioprotetores proporciona um excelente meio para criopreservacao do semen de caprinos. Novos estudos sao necessarios para verificar em que concentracao o aloe vera pode ser utilizado na criopreservacao do semen exercendo outros beneficios.

Niobium oxides are presently very important materials for electronic device applications. In this work, a new approach, using the proteic sol–gel route, was used to prepare niobium pentoxide powders. This approach uses powdered coconut water (PCW) for the powders production. The obtained powders, with an amorphous structure, were heat-treated at different temperatures and their structural and morphological properties were studied and related to their dielectric properties. Dielectric measurements as a function of frequency (100 Hz–1 MHz) and temperature (200–400 K) were performed. The heat-treatment promotes the formation of crystalline structures, mainly Nb2O5 with orthorhombic structure, which transforms to monoclinic with treatments at 1000 °C. The formation of CaNb2O6 and NaNbO3 crystallites, in the samples treated at 800 and 1000 °C is related to the PCW base composition. The samples revealed a quasi-independence of the dielectric constant with temperature and frequency, presenting a $$\varepsilon^{\prime}$$ ≈ 70 and tan ≈ 0.006, at room temperature and 100 kHz.

Eucalyptus radiata é uma espécie vegetal originária da Austrália, ecologicamente importante e fonte de óleos essenciais (OE) com potenciais atividades biológicas como ação antiinflamatória, antioxidante, analgésica, redutora de fadiga, hipoglicemiante, inseticida e bactericida que em geral são atribuídas a composição bioquímica presente no OE, sobretudo ao composto marjoritário 1,8-cineol. Contudo, inexistem estudos investigando o possível potencial cicatrizante do OE de E. radiata, o que torna esta planta um importante alvo para novas formulações terapêuticas. Desse modo, o presente estudo teve por objetivo caracterizar o efeito cicatrizante do OE de E. radiata em feridas dermatológicas. Para tanto, foi elaborado um creme de uso tópico com OE de E. radiata, que foi submetido a caracterização físico-química e a análise in vivo (Wistar albinus). Como resultados pode-se evidenciar que o creme elaborado com o OE de E. radiata (1,5, 3,5 e 5%) apresentou aspecto homogêneo característico de emulsão fase oleosa/aquosa, sem presença de grumos, não apresentou alterações significativas no aspecto, cor e odor, quanto ao pH (3,82 - 3,90) e a densidade, não houve grandes variações entre as formulações. Portanto, a utilização do OE de E. radiata na formulação de um creme apresentou-se ser viável, mantendo o odor característico do óleo, adequada espalhabilidade, não apresentou mudança de coloração, mantendo se constante sua estabilidade na maioria dos testes realizados. O gel creme a base de 5% do OE de E. radiata, neste trabalho, demonstrou ter potencial de estimular e favorecer o processo de cicatrização in vivo em ratos Wistar allbinus.

During the transition period in sheep, which consists of pre-partum and the beginning of lactation, the demands for glucose are extremely high and its absence or reduction causes several metabolic problems to arise leading to economic losses. Looking for alternative sources to growth promoters, sources of energy precursors of natural origin are being studied. This study aims to evaluate the effects and influence on plasma levels of Glucose, BHB and NEFA in pregnant ewes supplemented with coconut powder (ACP®) during the transition period. Two groups of ewes were used, totaling 13 animals of the Santa Inês breed that were supplemented with 30 g of powder coconut water (ACP) per day from 110 days before calving to 60 days after calving. Blood samples were taken on the day of delivery, 7, 21, 30 and 60 days after delivery. It was possible to observe that the test group (ACPg) remained stable with animals maintaining glucose levels without showing changes, even on the day of delivery, despite the fact that 80% of the ewes had twin births. The levels of BHB and NEFA were also better compared to the control group (Cg). The Cg showed greater instability throughout the experiment, with moments of hyper and hypoglycemia, BHB and NEFA also showed alterations. Lambs from ACPg showed better carcass growth compared to Cg. Therefore, the collection of results support the idea that, supplementation of ACP to pregnant ewes could be an important tool to reduce negative energy balance and offspring development in pregnant ewes.

As parasitoses gastrintestinais são os principais obstáculos enfrentados em caprinos, contribuindo para o aumento da mortalidade e consequente queda na produção animal. A presente pesquisa objetivou identificar a prevalência de helmintos gastrintestinais em caprinos de um município do Estado do Piauí. Trata-se de uma pesquisa experimental de natureza quantitativa. Constituiu a população alvo desta pesquisa, 45 caprinos sem raça definida provenientes de uma fazenda situada na zona rural do município de Teresina-PI. Esses animais receberam alimentação e sal mineral, de acordo o padrão recomendado tecnicamente para utilização no rebanho da propriedade. Os 120 animais selecionados para o estudo foram submetidos a exames coproparasitológicos e coprocultura. Independente de sexo, os animais possuem em média dois anos de idade, com peso médio de 38kg ± 500 gr. As fezes para a realização da análise coproparasitológica foram colhidas da ampola retal de cada animal. Os resultados apontaram a prevalência 84% de Haemonchus sp, 0,3% Oesophagostomun sp e 13% de Trichostrongylus sp. Considera-se então, relevante um esquema de vermifugação conforme um protocolo de um profissional, contemplando a região, hábitos, e demais características para direcionamento correto de vermífugos, no caso de soluções específicas.

The physical properties of the cubic and ferrimagnetic spinel ferrite LiFe5O8 has made it an attractive material for electronic and medical applications. In this work, LiFe5O8 nanosized crystallites were synthesized by a novel and eco-friendly sol-gel process, by using powder coconut water as a mediated reaction medium. The dried powders were heat-treated (HT) at temperatures between 400 and 1000 &deg, C, and their structure, morphology, electrical and magnetic characteristics, cytotoxicity, and magnetic hyperthermia assays were performed. The heat treatment of the LiFe5O8 powder tunes the crystallite sizes between 50 nm and 200 nm. When increasing the temperature of the HT, secondary phases start to form. The dielectric analysis revealed, at 300 K and 10 kHz, an increase of &epsilon, &prime, (&asymp, 10 up to &asymp, 14) with a tan&delta, almost constant (&asymp, 0.3) with the increase of the HT temperature. The cytotoxicity results reveal, for concentrations below 2.5 mg/mL, that all samples have a non-cytotoxicity property. The sample heat-treated at 1000 &deg, C, which revealed hysteresis and magnetic saturation of 73 emu g&minus, 1 at 300 K, showed a heating profile adequate for magnetic hyperthermia applications, showing the potential for biomedical applications.

Systemic lupus erythematosus (SLE) is a complex chronic autoimmune disease characterized by tissue damage and widespread inflammation in response to environmental challenges. Deposition of immune complexes in kidneys glomeruli are associated with lupus nephritis, determining SLE diagnosis. Periodontitis is a chronic inflammatory disease characterized by clinical attachment and bone loss, caused by a microbial challenge – host response interaction. Deposition of immune complex at gingival tissues is a common finding in the course of the disease. Considering that, the primary aim of this study is to investigate the deposition of immune complexes at gingival tissues of SLE patients compared to systemically healthy ones, correlating it to periodontal and systemic parameters. Twenty-five women diagnosed with SLE (SLE+) and 25 age-matched systemically healthy (SLE–) women were included in the study. Detailed information on overall patient's health were obtained from file records. Participants were screened for probing depth (PD), clinical attachment loss (CAL), gingival recession (REC), full-mouth bleeding score (FMBS) and plaque scores (FMPS). Bone loss was determined at panoramic X-ray images as the distance from cementenamel junction to alveolar crest (CEJ-AC). Gingival biopsies were obtained from the first 15 patients submitted to surgical periodontal therapy of each group, and were analyzed by optical microscopy and direct immunofluorescence to investigate the deposition of antigen-antibody complexes. Eleven (44%) patients were diagnosed with active SLE (SLE-A) and 14 (56%) with inactive SLE (LES-I). Mean PD, CAL and FMBS were significantly lower in SLE+ than SLE–(p< 0.05; Mann Whitney). The chronic use of low doses of immunosuppressants was associated with lower prevalence of CAL >3 mm. Immunofluorescence staining of markers of lupus nephritis and/or proteinuria was significantly increased in SLE+ compared to SLE–, even in the presence of periodontitis. These findings suggest that immunomodulatory drugs in SLE improves periodontal parameters. The greater deposition of antigen-antibody complexes in the gingival tissues of patients diagnosed with SLE may be a marker of disease activity, possibly complementing their diagnosis.

Purpose: The aim of this study was to evaluate the physical and chemical characteristics of coconut water and to analyze the use of coconut water solution for the conservation of human corneas. Methods: This was an experimental and controlled study performed at the Eye Bank of the General Hospital of Fortaleza. The coconut water-based solution was prepared at the Goat Seed Technology Laboratory of the Department of Veterinary Medicine of the State University of Ceará. Discarded corneas from the Eye Bank were divided into two groups for sequential experiments: G1, coconut water-based solution (experimental group), and G2, conservative treatment with OPTISOL GS® (control group). The osmolality of corneas in G1 was analyzed sequentially at 275, 300, 325, 345, 365, and 400 mOsm/L. The viability of the corneas was determined by specular microscopy and biomicroscopy on the first, third, and seventh days. Results: Corneas preserved in a solution of 365 and 345 mOsm/L had a transparency of 8 mm until the third day and had diffuse edema in the periphery, central folds, and partial epithelium loss until the seventh day. The 365-mOsm/L solution was associated with the worst results during follow-up. Corneas placed in Optisol-GS retained their original aspects. Conclusions: Coconut water-based preservative partially maintained corneal transparency and epithelial integrity, especially during the first three days of follow-up. The coconut water-based solutions used were not effective for use as preservatives in a human eye bank. RESUMO Objetivos: As características físico-químicas e o baixo custo da água de coco foram fundamentais para o este estudo. Analisar o uso de solução a base de água de coco como meio de conservação de córneas humanas em banco de olhos. Métodos: Estudo experimental e controlado realizado no Banco de Olhos do Hospital Geral de Fortaleza. Utilizou-se solução à base de água de coco preparada no laboratório de Tecnologia de Sêmen de Caprinos do Departamento de Medicina Veterinária da Universidade Estadual do Ceará. Foram usadas córneas de descartes divididas em dois grupos: G1 (Conservante com água de coco) - grupo experimental e G2 (grupo Conservante com OPTISOL GS®) grupo controle, em experimentos sequenciais. A osmolaridade do G1 foi analisada sequencialmente com 275, 300, 325, 345, 365 e 400 mOsm/L. A viabilidade das córneas foram realizadas por microscopia especular e biomicroscopia nos 1º, 3º e 7º dias. Resultados: As córneas em solução de 365 e 345 mOsm/L apresentavam transparência nos 8mm centrais até o 3º dia, com edema em toda periferia, dobras centrais e edema 2+, com perda parcial do epitélio até 7º dia, sendo o de maior osmolaridade com melhor transparência durante o seguimento. Grupo com 275, 300 e 400 mOsm/L, córnea opaca, edema difuso, perda total do epitélio no 3º dia. As córneas em Optisol mantiveram seus aspectos. Conclusões: O conservante à base de água de coco manteve em parte a transparência corneana e a integridade epitelial, especialmente nos primeiros 3 dias de seguimento. A solução conservante com água de coco nas formulações utilizadas não se mostrou eficaz para o uso em banco de olhos humanos.

Background: Sperm sexing aims to separate sperm populations in carriers of the “X” or “Y” chromosome. Currently, flow cytometry is a technique that allows greater accuracy; however, it causes structural changes in sperm, reduces viability, and has a high cost. As a result, other methods have been researched, including immunosexing, which uses monoclonal antibodies to detect sex-specific surface antigens. Thus, the objective of this study was to evaluate the immunosexing technique using a monoclonal antibody against sex-specific protein (HY) in the conservation of ram and goat semen in ACP101/102c.Materials, Methods & Results: Ejaculates from 5 rams and 5 goats were collected with the aid of an artificial vagina; they were evaluated and submitted to the immunosexing protocol, according to the manufacturer's recommendations, using the Monoclonal Antibody Kit specific for mammalian sperm with “Y” chromosomes (HY; HY Biotechnology, Rio de Janeiro, RJ, Brazil). After sexing, the supernatant was resuspended in the cryopreservation diluent: ACP ram (ACP101/102c + 20% egg yolk + 7% glycerol) and ACP goat (ACP101/102c + 2.5% egg yolk + 7% glycerol), packaged in 0.25 mL straws, refrigerated at 4°C, stabilized for 30 min, frozen in liquid nitrogen vapor (-60°C) for 15 min, immersed in liquid nitrogen, and stored in cryogenic cylinders. The samples were evaluated in natura (T1), after immunosexing (T2) and after thawing (T3) for sperm motility subjectively using conventional microscopy (40x). Plasma membrane integrity (IMP) and sperm cell morphology were evaluated by the smear staining technique using eosin-nigrosine dye, and the percentages of healthy and morphologically defect spermatozoa were determined. In the evaluation of ram semen regarding sperm motility and IMP, no statistically significant differences were observed between treatments after sexing in the evaluation of absolute data (P > 0.05), with the difference being observed only between T1 and T2, and T3 (P < 0.05). Regarding the relative percentage and sperm morphology, no statistically significant differences were observed (P > 0.05). Regarding the evaluation of goat semen samples, the motility parameters were consistent with the technique submitted; however, the IMP data did not appear as expected, requiring further evaluation for a better assessment of the technique for this species.Discussion: The data obtained from ram semen submitted to the immunosexing protocol, regarding the absolute evaluation of motility and IMP, demonstrated that the non-sexed semen (T1) was superior to the sexed treatments (T2 and T3); however, it is noteworthy that freezing started with approximately 50% of the cells, since the immunosexing technique results in a loss of viability of approximately 50% of the sperm, which corresponds to the ratio of sperm carrying the X chromosome. In addition, when the data in this study were transformed into relative values, no statistical differences were observed, indicating that the immunosexing protocol, as well as the freezing protocol, did not significantly affect the quality of ram sperm cells. In relation to the immunosexing of goat semen, future studies should be conducted in vitro to define a more appropriate protocol for the species and, in addition, in vivo studies should be performed to prove the quality of the technique. It was concluded that the immunosexing process using a monoclonal antibody against sex-specific protein (HY) associated with the use of powdered coconut water diluent (ACP101/102c) in the cryopreservation of semen proved to be efficient in the in vitro evaluation of ovine species.

Introdução: A má postura caracteriza-se pela relação defeituosa entre as várias partes do corpo que produz uma maior tensão nas estruturas de apoio. Objetivo: Comparar a prevalência de alterações posturais da coluna vertebral em alunos do ensino fundamental de duas escolas, uma da rede privada e outra da rede pública, do municí­pio de Quixadá/CE. Métodos: Avaliaram-se 40 alunos voluntários de ambas as escolas, sendo 48% do sexo masculino e 52% do sexo feminino, com idades entre 11 e 15 anos. Analisaram-se com ajuda de bolas de isopor sobrepostas nos principais relevos anatômicos e em seguida fotografias, nas quatro vistas, anterior, posterior, lateral esquerda e lateral direita, de forma estática, posicionados atrás do simetrógrafo, analisando através do software Coreldraw, marcando as alterações mais evidentes. Resultados: Demonstraram a saúde deficiente da coluna vertebral em escolares, com a identificação de várias alterações posturais, sendo mais prevalente na instituição da rede municipal de Quixadá/CE. Sendo elas: hipercifose, desequilí­brio pélvico, desequilí­brio escapular, inclinação lateral cervical, anteriorização cervical e elevação unilateral do ombro. Entretanto, os alunos da instituição da rede privada apresentaram as mesmas alterações, sendo em um menor í­ndice. Conclusão: Observou-se que o fator socioeconômico das instituições e dos alunos que as compõe pode provocar futuras alterações posturais, devido às mobí­lias e a forma de transporte de materiais serem inadequadas. O estudo sugere a presença de um fisioterapeuta no ambiente escolar onde seria possí­vel intervir na evolução dessas alterações e a fixação dos desvios, visando o trabalho preventivo e educacional.Palavras-chave: postura, epidemiologia, escoliose, Fisioterapia.

A acne vulgar é uma dermatose crônica do folículo pilossebáceo, que possui, como fatores fundamentais, hiperprodução sebácea. Não existe perfil epidemiológico universal para a mesma. Atinge ambos os gêneros e é um problema de saúde pública. O objetivo deste trabalho foi avaliar a eficácia do tratamento do gel da Camomila (Matricaria chamomilla L.) associado ao LED Vermelho de baixa frequência (4,82 – 3,84Hz) no tratamento da Acne Vulgar. Trata-se de um estudo de campo, experimental com abordagem quanti – qualitativa incluindo 20 pacientes do gênero feminino atendidas em uma clínica de Fisioterapia Dermatofuncional da Cidade de Floriano-PI. As pacientes foram alocadas em grupos de cinco mulheres cada. Para avaliação do estado da pele foram utilizadas a Escala Visual de Percepção facial - EVPF e a avaliação por Contagem no Número de Lesão – CNL. Ao fim do estudo constatou-se que o tratamento da acne com uso do gel da camomila a 5% associada ao aparelho de LED Vermelho de baixa frequência (4,82 – 3,84Hz), mostrou-se efetivo na diminuição do processo inflamatório da pele. Apresentando-se como uma terapêutica segura, acessível e eficaz. Sendo também importante outros cuidados especiais com a pele nos pacientes com essa afecção dermatológica.

Objetivou-se avaliar o efeito do extrato de bromelina sobre a qualidade espermática, pós-descongelação na espécie caprina. Para tanto, foram utilizados cinco caprinos da raça Anglo Nubiana, com idade reprodutiva, clinicamente saudáveis. Foram realizadas colheitas de sêmen. Após a análise da concentração, o volume total do pool foi dividido em cinco grupos. Um pertencente ao grupo controle, composto de (ACP-101/102®) e quatro grupos experimentais com ACP-101/102® enriquecidos com extrato de bromelina nas concentrações de 5%; 10%; 15% e 20%. As amostras foram criopreservadas com auxílio do aparelho Tk3000®. Após o período de sete dias as amostras foram descongeladas e submetidas a avaliações pelo sistema CASA. Termo resistência, sondas fluorescente, teste cometa e teste de análise ultraestrutural de espermatozoides por meio de microscopia eletrônica de transmissão (MET). Na avaliação da cinética espermática pós-descongelação foi possível observar que os parâmetros motilidade espermática total, o grupo bromelina a 5%, se destacou dentre os demais, já na velocidade curvilínea e velocidade média da trajetória o grupo controle apresentou os melhores resultados. Quanto à integridade do DNA espermático as concentrações de bromelina (5%; 10%; 15% e 20%) não apresentaram diferença significativa em relação ao grupo controle, demonstrando que essa substância não apresenta genotoxicidade às células espermáticas. Na análise ultraestrutural o grupo bromelina a 5% demonstrou os melhores resultados. Nesse sentido, o extrato de bromelina adicionada a meios diluentes, visando à criopreservação, caracteriza-se como uma substância promissora, sobretudo da manutenção da integridade do DNA espermático. Contudo, mais estudos são necessários para a sua padronização.

Um meio diluente ideal para a criopreservação seminal deve suprir as células espermáticas com energia, proteção e manutenção de um ambiente adequado à sua sobrevivência. Objetivou-se avaliar a integridade in vitro do sêmen caprino criopreservado em meio ACP-101c® associado à gema de ovo de Numida meleagris, por meio de duas técnicas de análise individual da célula espermática. Foram colhidos 15 ejaculados de cinco caprinos, com auxílio de uma vagina artificial. Os ejaculados foram reunidos em um pool, e dividido em 12 grupos, sendo dois grupos controles: GC1 TRIS, com adição 2,5% da gema de ovo de Gallus gallus domesticus GOGD, GC2 ACP-101c®, com adição 2,5% da gema de ovo de Gallus gallus domesticus GOGD e dez grupos experimentais GE, contendo a adição da gema de ovo de Numida meleagris. Seguidamente, as alíquotas foram envasadas em palhetas francesas de 0,25 mL e congeladas com auxílio do aparelho TK3000® e armazenadas em nitrogênio líquido. Amostras foram descongeladas após sete dias e avaliadas quanto à integridade do DNA e quanto a sua ultraestrutura individual, por meio do teste cometa e microscopia eletrônica de transmissão, respectivamente. Nos resultados obtidos pelo teste cometa, não foi evidenciado diferença estatística quanto ao comprimento da cauda, entre os grupos TRIS acrescido de GONM, nas concentrações de 2,5%, 5% e 10% em relação ao grupo controle TRIS 2,5% de GOGD. Também não houve diferença estatística quanto ao percentual de fragmentação de DNA na cauda, nos grupos TRIS com 2,5%; 5%; e 20% de GONM em comparação ao grupo controle TRIS 2,5% GOGD (P>0,05). Os grupos ACP® com 10%, 15% e 20% de GONM apresentaram maior comprimento de cauda quando comparados aos grupos ACP com 2,5% e 5% de GONM e grupo controle (ACP® 2,5% de GOGD). Na análise ultraestrutural o grupo ACP® com 10% de GONM, destacou-se com melhor integridade celular frente aos demais grupos, inclusive, frente às amostras avaliadas do grupo controle. Dessa forma, conclui-se que a gema de ovo de Numida meleagris, como crioprotetor externo de membrana, acrescida aos diluentes ACP-101c® ou TRIS, pode reduzir os danos causados durante o processo de criopreservação do sêmen caprino.

Background : Sperm sexing is increasing in use because pre-determining the sex of the calf allows greater profitability and promotes significant gains in the productive systems that utilize the technique. Deployment of a low-cost and practical preservation methodology may further favor the cost-benefit ratio. Flow cytometry, the most commonly used sexing technique, has high costs and is very restricted. As an alternative, immunosexing has been studied, which uses sex-specific monoclonal antibodies. Thus, the objective of this study was to evaluate the immunosexing technique in conjunction with cryopreservation in ACP-102c and examine its economic aspects with regard to ram semen. Materials, Methods & Results : Ejaculates from two ram individuals were collected with the aid of an artificial vagina, evaluated, and submitted to the immunosexing protocol, according to the manufacturer's recommendations, using the Monoclonal Antibody Kit specific for mammalian sperm with “Y” chromosomes (HY; HY Biotechnology, Rio de Janeiro-RJ, Brazil). After sexing, the supernatant was resuspended in the cryopreservation diluent: ACP (ACP-102c + 20% egg yolk + 7% glycerol), packaged in 0.25 mL straws, refrigerated to 4°C, stabilized for 30 min, frozen in liquid nitrogen vapors (-60°C) for 15 min, immersed in liquid nitrogen, and stored in cryogenic cylinders. The samples were thawed and evaluated for sperm kinetics both by using computerized semen analysis with SCA ® software ( Sperm Class Analyzer version 5.0) and subjectively comparing specimens from the two animals using conventional microscopy (40x). Plasma membrane integrity (IMP) and sperm cell morphology were evaluated by the smear staining technique using eosin-nigrosine dye, and the percentages of healthy and normal spermatozoa were determined. A bibliographic survey and a market study of similar products and technologies were carried out to provide an economic viability metanalysis of the bioproduct (ACP-102c) and bioprocess (immunosex). The data were analyzed using R-project © , and comparisons made between animals and between thawing periods using T test . There were no statistically significant differences between animals and between periods ( P > 0.05), except for the normal sperm parameter, in which animal A1 had the lowest percentage ( P < 0.05). As for the cost-benefit ratio, flow cytometry as a technique is more laborious and expensive, while immunosexing associated with cryopreservation in ACP-102c diluent has proven more practical, with regards to both sperm sexing techniques and diluents for sperm conservation. Discussion: In general, the quality of cryopreserved sexed semen was lower than that of non-sexed semen; however, in this study, both in the comparison between animals and between evaluation periods, similar values of motility, viability, and sperm morphology were obtained for sexed and several non-sexed cryopreserved semen samples, demonstrating that immunosexing did not severely affect the sperm structure, and that the ACP-102c conservation medium was efficient at maintaining the plasma membrane of these sperm. In the evaluation of economic aspects, it was observed that immunosexing, associated with cryopreservation in ACP-102c diluent, proved to be the most practical technique, requiring only conventional equipment, and allowing a greater field of application, since the immunosexing semen can be used for primiparous and multiparous females. Thus, it was concluded that immunosexing associated with cryopreservation in an ACP-102c diluent was more cost-effective, more practical, and had significantly improved sperm quality results after sexing.

Pigmented purpuric dermatoses (PPD) include a spectrum of diseases with different clinical aspects, but with similar histopathological features. Specific clinical findings allow the division of PPD in variants. Schamberg's disease is the most common. Treatment is sometimes ineffective and recurrences are common. There are reports of patients who responded well to the use of colchicine. We report the case of a 32-year-old woman, previously healthy, with a history of onset of asymptomatic lesions in legs. She presented purpuric skin eruptions and brownish stains diffusely distributed in the lower limbs. Biopsy was compatible with PPD. We decided for the introduction of colchicine, with good clinical response. The patient has been followed on outpatient basis for ten months without recurrence.

Made available in DSpace on 2019-10-04T12:38:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-07-01 The aim of this study was to evaluate the effect of coconut water as a component of extender in different formulations for cooling equine sperm. One ejaculate of fourteen stallions was collected. Sperm was diluted to 50 x 10(6) sperm/mL using five different extenders: ACP-105: powdered coconut water extender (ACP-105, ACP Biotecnologia, Brazil); ACP-Milk: ACP-105 + 20 g/L of skimmed milk; ACP-EY 2.5%: ACP105 + 2.5% of egg yolk; ACP-EY 5%: ACP-105 + 5% of egg yolk; and BotuSemen (Botupharma, Botucatu, Brazil) and cooled in passive cooling device (BotuFlex, Botupharma, Botucatu, Brazil) at 5 and 15 degrees C for 24 hours. Sperm kinetics and plasma membrane integrity (PMI) were evaluated by computer-assisted sperm analysis and fluorescence staining, respectively, at TO (before cooling) and T24 (24 hours after cooling). Sperm kinetics did not differ at TO among groups (P > .05); however, at T24, these parameters were significantly lower in ACP-105 (5 degrees C, total motility [TM]: 9.2 +/- 3.6%; progressive motility [PM]: 2.7 +/- 1.6%; percentage of fast-moving spermatozoa [RAP]: 4.8 +/- 3.0%; 15 degrees C, TM: 10.6 +/- 3.0%; PM: 1.1 +/- 0.5%; RAP: 4.8 +/- 1.9%) and ACP-EY 5% (5 degrees C, TM: 28.0 +/- 6.3%; PM: 5.7 +/- 1.8%; RAP: 15.9 +/- 6.0%; 15 degrees C, TM: 30.0 +/- 6.0%; PM: 6.9 +/- 2.1%; RAP: 17.6 +/- 5.3%) compared with BotuSemen (5 degrees C, TM: 66.2 +/- 5.6%; PM: 21.1 +/- 2.8%; RAP: 53.9 +/- 6.1%; 15 degrees C, TM: 63.4 +/- 5.4%; PM: 17.2 +/- 2.8%; RAP: 51.4 +/- 6.3%) (P < .05). All groups exhibited similar PMI at tested moments and cooling temperatures (5 degrees C: 83%; 15 degrees C: 84%) (P> .05). Further studies are necessary to evaluate powdered coconut water in different compositions of sperm extender; however, coconut-based extender as used in this study was not an alternative to preserve sperm parameters of cooled equine sperm. (C) 2019 Elsevier Inc. All rights reserved. Univ Estadual Ceara, Dept Vet Med, Fortaleza, Ceara, Brazil Sao Paulo State Univ, Dept Anim Reprod & Vet Radiol, Sch Vet Med & Anim Sci, Botucatu, SP, Brazil Sao Paulo State Univ, Dept Anim Reprod & Vet Radiol, Sch Vet Med & Anim Sci, Botucatu, SP, Brazil

The objective of the current study was to observe the performance kinetics (motilities and velocities) of the spermatozoa from Prochilodus brevis (curimatã), Colossoma macropomum (tambaqui) and Piaractus brachypomus (pirapitinga) species in different times post-activation. The sperm of P. brevis, C. macropomum and P. brachypomus species were collected after hormonal induction with carp pituitary extract. The samples with not contamination with water, urine or feces had motility subjective, morphology, osmolality and concentration analyzed. The samples selected were analyzed with Sperm Class Analyzer. Spermatozoa motility and velocities were captured at 10, 30, 60 and 120 s post-activation. No significant differences in total motility of P. brevis spermatozoa were observed between 10 s and 30 s post-activation. However, significant reduction was observed in 60 s. This reduction was more accentuated after 120 s. The same pattern of spermatozoa motility decline happened for C. macropomum and P. brachypomus. Velocities also followed the same pattern for the three species. There was significant reduction in velocities after 30 s; this reduction was more significant after 60 s. There was no significance difference between 60 s and 120 s post-activation. Sperm of C. macropomum and P. brachypomus show satisfactory sperm quality up to 60 s after activation. On the other hand, sperm of P. brevis up to 120 s after activation. These findings show that the rate of sperm motility in different times post activation is change for each species tested.

This study aimed to evaluate the extract of Aloe vera (AV) associated or not with 10% Dimethylsulfoxide (DMSO) in cryopreservation of tambaqui semen. For the formation of the pools (n= 14), 30 males were hormonally induced twice. Each pool had the objective motility, curvilinear velocity, straight-line velocity, average path velocity and morphology analyzed before and after cryopreservation of semen. The means for cryopreservation were constituted of Powder Coconut Water-104 diluent added DMSO and/or AV (5 or 10%). After cryopreservation, motility, velocities and morphology were reduced significantly when compared to fresh semen. For sperm motility the best treatment was that using only DMSO (20,86±8,31) and DMSO + 5% AV (15.71±9.77). For the velocities, the worse treatment was DMSO+10% AV. Treatment with only the addition of DMSO had a significantly higher effect than others on percentage of morphologically normal sperm. The mean correlation found was between motilityand the rate of morphologically normal sperm (r = 0.687). In conclusion, the addition of AV does not provide greater protection for spermatozoa during cryopreservation.

SUMMARY The aim of this study was to evaluate the effects of the protein-calorie malnutrition in BALB/c isogenic mice infected with Lacazia loboi, employing nutritional and histopathological parameters. Four groups were composed: G1: inoculated with restricted diet, G2: not inoculated with restricted diet, G3: inoculated with regular diet, G4: not inoculated with regular diet. Once malnutrition had been imposed, the animals were inoculated intradermally in the footpad and after four months, were sacrificed for the excision of the footpad, liver and spleen. The infection did not exert great influence on the body weight of the mice. The weight of the liver and spleen showed reduction in the undernourished groups when compared to the nourished groups. The macroscopic lesions, viability index and total number of fungi found in the footpads of the infected mice were increased in G3 when compared to G1. Regarding the histopathological analysis of the footpad, a global cellularity increase in the composition of the granuloma was observed in G3 when compared to G1, with large numbers of macrophages and multinucleated giant cells, discrete numbers of lymphocytes were present in G3 and an increase was observed in G1. The results suggest that there is considerable interaction between Jorge Lobo's disease and nutrition.