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IntroductionThe clitoris is partially responsible for sexual arousal. The integrity of the extracellular matrix is essential for clitoral erection. Sexual dysfunction is a phenomenon associated with age.Material and methodsThe clitoris of cadavers of 20- to 80-year-old women was excised and histologically processed. Stereological analysis was performed to quantify the volumetric density of collagen, elastic fibers, and smooth muscle.ResultsA significant increase in collagen and a decrease in smooth muscle and elastic fibers were observed in older women.ConclusionsIn short, these changes caused by aging could contribute to female sexual dysfunction concerning clitoral orgasm.

IntroductionIt is known that the quality and quantity of milk is directly related to the dietary habits of the mother. Despite that, the rates of maternal malnutrition during lactation are increasing in several countries; thus, observing its effects on the offspring is relevant. The present study aims to verify the effects of maternal malnutrition during breastfeeding on the skulls of adult-aged Wistar rats.Material and methodsThirty-six newborn rats were divided in three groups: the control group, in which the mother received a regular commercial diet containing 23% protein in unlimited amounts; the protein-energy restriction group, in which the dam received a commercial diet containing 8% protein in unlimited amounts; the energy restricted group, in which the dam received a commercial diet containing 23% of protein in limited amounts. After weaning, all rats received the same diet as the control group until 180 days of age. Then, the rats were euthanized, and their crania were excised and measured in radiographic images. Afterwards, their skull was decalcified with nitric acid (5%) and histological samples were obtained and the thickness of the diploe was verified. Descriptive statistics and ANOVA followed by the Newman-Keuls test were performed for comparison purposes.ResultsIt was observed that the skull from the protein-energy restriction and energy-restriction groups was smaller and thinner than that of the control group in several parameters.ConclusionsMaternal malnutrition during the lactation period caused long-term effects in skull morphology of Wistar rats. These effects could not be reversed after regulation of the diet.

Introduction Knowledge of the prostatic portion of the urethra is essential to the comprehension of urinary continence phenomena. However, there are only a small number of studies that have addressed this relationship and analyzed the ultrastructure of the prostatic urethra. Material and methods A three-dimensional analysis of the fibrous components and the extracellular matrix of the prostatic urethra in 10 normal humans was performed with a scanning electron microscope. The prostates were obtained from 10 men (18 to 30 years old) who had died from accidents. The specimens were fixated in a modified Karnovsky solution for 48 h at 4°C, washed in a sodium phosphate buffered solution (PBS) for 2 h at 4°C and then incubated in 40 ml of 2 M NaOH at room temperature for 8 days (cellular digestion). Afterwards, the three-dimensional organization of the prostatic urethra stroma was observed. Results It was observed that the prostates had a narrowed fibrous septa which condensed and formed a network in order to support the submucosa. Furthermore, it was also observed that they formed a homogeneous fibrous layer (pars fibroreticularis), which lined the urethral wall. Conclusions Knowledge of the urethra composition is essential to understand the physiopathological aspects of urinary incontinence. Moreover, our results showed a great amount of connective tissue underlying the prostatic urethra wall.

Introduction: The Caroticoclinoid Foramen (CCF) is a variation found in the sphenoid. It gives passage to the internal carotid artery and it is a surgically significant structure when dealing with the cavernous sinus. There is debate, however, regarding its prevalence, especially in populations from South America. Aim: To assess the prevalence and size of the CCF in a Brazilian sample. Materials and Methods: This was a cross-sectional study carried out for a period of 10 months during March 2019 and January 2020. The present study was conducted in 365 dry human skulls from two Universities in the Southeast region of Brazil. The caroticolinoid foramen was observed and if present, measured. Results: Of the 365 skulls examined, the foramen was present in 101 (prevalence of 27.6%). The foramen was bilateral in 88 cases, while 13 skulls presented this variation unilaterally (7 on the right and 6 on the left side). Regarding its classification, out of 190 CCF analysed, 69 were pertaining to the complete type, while 120 cases were classified as incomplete and 1 from the contact type. The mean anteroposterior diameter was 4.87±0.69 mm (right side) and 4.86±0.79 mm (left side), and the Transverse Diameter (TD) was 4.85±0.75 mm (right side) and 4.74±0.73 mm (left side). Sixty-nine skulls had data regarding sex and age. The age ranged from 1 month old to 104-year-old (mean of 37.79±21.85-year-old). The male to female ratio was 2:1, being the only relation with statistical significance (p

The extracellular matrix (ECM) of the fallopian tubes is subject to several changes due to hormonal influences and aging. However, there is a lack of studies regarding its arrangement in older women. We aimed to analyze the organization of ECMcomponents, including collagen and elastic fibers, in the fallopian tube’s ampulla from young and old women by means of scanning electron microscopical and stereological methods. Twenty-one samples were analyzed: 12 from female cadavers in a fertile age (G1) and 9 from the climacteric period (G2). Masson’s trichrome stain was used to observe collagen and smooth muscle, while Weigert’s Fuchsin-Resorcin was employed to observe elastic fibers. Statistical analysis was performed by the Wilcoxon-Mann-Whitney test with the aid ofthe R(©) software. The tissue was also fixed for scanning electron microscopic analysis in a modified Karnovsky solution and the three-dimensional organization of fibrous connective tissue was observed and compared. Statistically significant differences (P < 0.01) were found in all stereologic comparisons of the extracellular matrix between the groups, which revealed a higher volumetric density of the fibrous tissue in the climacteric group. Scanning electron microscopy showed degenerative alterations of extracellular matrix. According to our results, aging caused significant changes to the elements of the extracellular matrix and the smooth muscle of the fallopian tubes.

The anterior vaginal wall is subject to many diseases, such as pelvic organ prolapse. The pathophysiology of this illness is multifactorial, and as such, structural components of the vagina are involved. Furthermore, it is more prevalent in older women. There is a lack of data in the literature regarding the extracellular matrix components of the vaginal wall and its changes with aging. The work presented herein aims to perform a stereological study of the extracellular matrix in young and old women. It was observed a decrease of the volumetric density of smooth muscle (45.5 ± 3.2 % and 32.8 ± 5.8 % for the G1 and G2 samples, respectively) and an increase of collagen and elastic fibers with age (35.9 ± 2.1 % and 54.1 ± 5.9 % for the G1 and G2, respectively) in the mucosa of the vaginal wall. These results could help to better understand the pathophysiology of pelvic organ prolapse concerning the aging process.

Variations of the forearm muscles are well described in the literature. In spite of that, reports regarding the accessory flexor carpi ulnaris muscle are scarce due to its rarity. This muscle usually originates from the medial epicondyle of the humerus with the flexor muscle mass and inserts itself into the palmar aponeurosis or the pisiform bone together with the flexor carpi ulnaris muscle. Supernumerary and anomalous muscles of the anterior compartment of the forearm have been associated with ulnar nerve and artery compression. Furthermore, they may also mimic neuromas and soft tissue tumors. The study of supernumerary muscles is necessary, as they may be an option to tendon grafts and muscle flaps. The aim of this paper is to describe the presence of the accessory flexor carpi ulnaris muscle on the left forearm of a cadaver fixated in a phenol solution and discuss its clinical and anthropological significance.

SUMMARY: The supracondylar process is a hook shaped projection of the humerus. It is located superior to its medial epicondyle. This anatomic variation is often presented together with the Struther’s ligament, a fibrous arcade that connects the supracondylar process to the medial epicondyle. Both structures have been associated with neurovascular bundle compression on the distal third of the arm, as the median and ulnar nerves and the brachial and ulnar arteries may pass through the osteofibrous canal that these variants create. The SciElo, Pubmed, Scopus, TRIP, MEDLINE, COCHRANE and ScienceDirect databases were searched with the term “supracondylar process” or “supracondyloid process” with the purpose of performing a meta-analysis of this bony spur. Heterogeneity between studies was evaluated using I2 estimation and the Cochran Q statistic test. A random effect model was used for all analysis. A total of twenty articles (26.415 humeri) were included in this meta-analysis. The pooled prevalence of the supracondylar process was 0.68 % (95 % Confidence Interval: 0.47 % to 0.92 %). This variant was more commonly found in women than in men (statistically significant difference) and more commonly found on the left side than the right. The presence of the supracondylar process alone may induce neurovascular bundle compression. It can also be injured in traumatic or stress fractures. Knowledge of this variation and its prevalence may reduce misdiagnosis in radiographic images.

Variations of the forearm musculature are vastly described in the literature. The palmaris longus muscle is one of the most variable structures of the human body. It usually arises from the medial epicondyle of the humerus and inserts itself into the palmar aponeurosis. Anatomy textbooks and recent papers state that this muscle may act as a weak wrist flexor. The present work aims to report an anatomical variation where the palmaris longus muscle was abnormally hypertrophied and was completely fleshy throughout the whole forearm. Anatomical variations of the palmaris longus may induce symptoms of neurovascular bundle compression, especially of the median nerve. The palmaris longus muscle may be used in tendon graft or muscle graft, due to its lack of apparent function. Furthermore, it is one of the anatomical landmarks for local anaesthesia procedures. (Folia Morphol 2018; 77, 2: 403-405).

El objetivo de esta investigacion fue analizar y cuantificar los cambios de las fibras reticulares en la uretra prostatica de pacientes con hiperplasia prostatica benigna (HPB) y compararlo con un grupo control. Muestras de tejido de uretra prostatica se obtuvieron de diez pacientes (rango de edad 65 a 79 anos, media 66) con sintomas clinicos de obstruccion del tracto urinario inferior que se habian sometido a prostatectomia abierta. Las diez muestras del grupo de control (muestras uretrales de tejido de la zona de transicion) se obtuvieron de prostatas durante la autopsia de sujetos adultos con muerte accidental menores de 25 anos. El Vv de las fibras reticulares se determino con metodos estereologicos de 25 campos al azar por muestra utilizando el metodo de valor de conteo con el sistema M-42. Los datos cuantitativos se analizaron mediante la prueba de Kolmogorov-Smirnov y Mann-Whitney U. El Vv (media ± DE) en el grupo control y BPH, respectivamente, fueron: 23,4 ± 1,8 y 30,3 ± 1,2 (0,001). La BPH causo aumento significativo de fibras reticulares en uretra prostatica.

El musculo gemelo superior (SGM) que se origina de la superficie externa de la parte posterior del isquion, se une a la parte superior del tendon del musculo obturador interno, y se introduce con el en la superficie medial del trocanter mayor. En este trabajo se describe una variacion anatomica en la que el SGM esta ausente bilateralmente. Debido a la escaza descripcion de esta variacion, es relevante conocer la ausencia de la SGM, con el fin de mejorar el conocimiento anatomico y clinico de este hallazgo.

The first cervical vertebra is subject to numerous anatomical variations. One of these is posterior arch agenesis, which is classified into five distinct morphological types. Together, all types of posterior arch agenesis comprise only 4% of atlas variations. Furthermore, complete agenesis of the posterior arch associated with the presence of the posterior tubercle is rare. This work reports a case of posterior arch agenesis with the presence of the posterior tubercle in a 33 year-old male victim of a motor vehicle collision. Despite being asymptomatic, this anatomical variation can present with headaches and neck pain. It is mostly found as an incidental finding in imaging studies performed by the emergency team and, as a result, it is often misdiagnosed as a C1 fracture. Knowledge of the variations relating to the first cervical vertebra is therefore essential to avoid delays in diagnosis and treatment of polytraumatized patients. Level of evidence V; Case report.

Background The aim of this study was to evaluate the leptin levels in the serum and peritoneal fluid (PF) and the protein expression in three different peritoneal ectopic implants in patients who underwent surgery for deep infiltrating endometriosis. Methods All patients had been treated at the Department of Gynecology of the Pedro Ernesto University Hospital, Rio de Janeiro. The study group consisted of 15 patients who underwent surgery for adnexal masses and infertility, while the control group consisted of ten women who underwent surgery for tubal ligation. Peritoneal fluid and samples tissues were collected during surgery. Serum samples were obtained before anesthesia. In this study, the leptin levels in the serum and peritoneal fluid (PF) were evaluated by ELISA. The protein expression of leptin and its receptors (ObR) and aromatase enzyme were evaluated by Western blot analysis of the intestine, uterosacral ligament and vaginal septum in the ectopic implants. The t-test and one-way ANOVA with Holm-Sìdak post-test were used, and p