14 results on '"Joonhaeng Lee"'
Search Results
2. Effectiveness of Sodium Iodide Root Canal Filling Pastes in Primary Teeth
- Author
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Soo Jin Chang, Yujin Kim, Junghwan Lee, Jongsoo Kim, Joonhaeng Lee, Mi Ran Han, Jisun Shin, and Jongbin Kim
- Subjects
General Medicine - Abstract
Objectives: This study aimed to compare the physical properties and antibacterial effectiveness of iodoform based root filling pastes, Vitapex® and Metapex®, with sodium iodide root filling paste (NaI paste) for primary teeth. Materials and Methods: The physical properties (flowability, film thickness, radiopacity) of the pastes were evaluated according to ISO 6876:2012. The antibacterial activity against Enterococcus faecalis strain (ATCC 6538) was evaluated using a direct contact test. Results: There was no significant statistical difference (p > 0.05) observed in the flow and film thickness of NaI paste when compared to the currently available root canal filling materials. The average flow capacities for Vitapex®, Metapex®, and NaI paste were 15.40 mm, 21.25 mm, and 20.01 mm, respectively. The average film thickness for Vitapex®, Metapex®, and NaI paste were 33.3 μl, 22.6 μl, and 25.0 μl, respectively. However, NaI paste showed lower radiopacity than the existing materials, and this difference was statistically significant (p < 0.05) NaI paste demonstrated higher antimicrobial activity than the available materials, and this difference was also statistically significant (p < 0.05). Conclusion: Compared to the existing commercialized root canal filling materials, NaI paste exhibited similar performance in terms of flow and film thickness, and superior antimicrobial activity against E. faecalis . Hence, NaI paste could be a promising root filling material for primary teeth and may be a potential alternative to existing materials.
- Published
- 2023
3. Evaluation of Setting Time, Solubility, and Compressive Strength of Four Calcium Silicate-Based Cements
- Author
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Yuji Jang, Yujin Kim, Junghwan Lee, Jongsoo Kim, Joonhaeng Lee, Mi Ran Han, Jongbin Kim, and Jisun Shin
- Subjects
General Medicine - Abstract
This study aimed to compare the physical properties of 4 kinds of calcium silicatebased cements (CSCs): 2 kinds of powder-liquid mix type (RetroMTA® [RTMX] and Endocem® MTA Zr [EZMX]) and 2 kinds of premixed type (Well-Root™PT [WRPR] and Endocem® MTA premixed [ECPR]) CSCs, respectively. Further, we assessed the setting times, solubility values, and compressive strengths of the cements. The shortest setting time was observed for EZMX (123.33 ± 5.77 seconds), followed by RTMX (146.67 ± 5.77 seconds), ECPR (260.00 ± 17.32 seconds), and WRPR (460.00 ± 17.32 seconds), respectively. The highest solubility was observed for WRPR (9.01 ± 0.55%), followed by RTMX (2.17 ± 0.07%), EZMX (0.55 ± 0.03%), and ECPR (0.17 ± 0.03%). Furthermore, the highest compressive strength was observed for ECPR (76.67 ± 25.67 Mpa), followed by WRPR (38.39 ± 7.25 Mpa), RTMX (35.07 ± 5.34 Mpa), and EZMX (4.07 ± 0.60 Mpa). In conclusion, the premixed type CSCs (WRPR and ECPR) exhibited longer setting times compared to the powder-liquid mix type CSCs (EZMX and RTMX). The solubility test showed that ECPR had the lowest solubility while WRPR had the highest solubility, with a statistically significant difference between them (p < 0.0083). Additionally, the compressive strength test showed that ECPR had the highest compressive strength, while EZMX had the lowest compressive strength, also with a statistically significant difference between them (p < 0.0083). ECPR is a promising material as it is premixed, eliminating the need for mixing time, and it has also demonstrated improved solubility and compressive strength, making it a potentially favorable option for clinical use.
- Published
- 2023
4. Comparison of Color Stability and Surface Roughness of 3D Printing Resin by Polishing Methods
- Author
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Heeju Kim, Yujin Kim, Jongsoo Kim, Joonhaeng Lee, Mi Ran Han, Jisun Shin, and Jongbin Kim
- Subjects
General Medicine - Abstract
This study aimed to compare the color stability and surface roughness of three-dimensional (3D) printing resin according to polishing methods. 3D-printed resin specimens were fabricated at TC-80DP (Graphy, Seoul, Korea) with a stereolithography 3D printer, and the specimens were divided into three groups, each of which was not polished, was polished using Enhance®, and was polished using a Sof-LexTM disc. The CIE L*a*b* value and the surface roughness of each group were measured and immersed in artificial saliva and orange juice after 0, 1, 7, 30, and 60 days, and the color difference (ΔE*) was calculated. As a result of the study, no noticeable color change was observed in artificial saliva, but a noticeable color change was demonstrated in orange juice after 60 days of immersion, and the difference was significant. In the Sof-LexTM group, surface roughness according to the solution was found to be significantly higher in the orange juice than that in artificial saliva. No significant difference in color change was found according to the polishing method, but surface roughness was significantly lower in the Sof-LexTM group than both that of the unpolished group and that of the Enhance® group. Nevertheless, all groups exhibited clinically acceptable properties regardless of their higher surface roughness than the threshold for plaque accumulation. Overall, this study recommends utilizing Sof-LexTM for polishing 3D printing resin when used in primary anterior tooth coverage.
- Published
- 2023
5. CareCall: a Call-Based Active Monitoring Dialog Agent for Managing COVID-19 Pandemic.
- Author
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Sang-Woo Lee 0001, Hyunhoon Jung, SukHyun Ko, Sunyoung Kim, Hyewon Kim, Kyoungtae Doh, Hyunjung Park, Joseph Yeo, Sang-Houn Ok, Joonhaeng Lee, Seongjae Choi, SeungTae Hwang, Eun-Young Park, Gwang-Ja Ma, Seok-Joo Han, Kwang-Seung Cha, Nako Sung, and Jung-Woo Ha 0001
- Published
- 2020
6. pH, Ion Release Capability, and Solubility Value of Premixed Mineral Trioxide Aggregates
- Author
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Seolah Back, YuJi Jang, Junghwan Lee, Joonhaeng Lee, Jisun Shin, Jongbin Kim, Miran Han, and JongSoo Kim
- Subjects
General Medicine - Abstract
The current study aimed to compare the pH, solubility value, and ion release capability of premixed mineral trioxide aggregates (MTAs) versus conventional pulp capping materials before and after setting. The following materials were used: resin-modified calcium silicate cement (TheraCal LC®, TLC), resin-modified calcium hydroxide cement (Ultra-BlendTM plus, UBP), and 2 kinds of premixed MTA (Endocem MTA® premixed regular 〔EMPR〕 and Well-RootTM PT 〔WRP〕). The specimens of each material were prepared before and after setting and were immersed in distilled water. The materials’ pH and solubility value were assessed. Next, three kinds of ion (calcium, sulfide, and strontium) released by pulp capping materials were evaluated via inductively coupled plasma atomic emission spectrometry. In the after-setting group, the pH of TLC and UBP decreased. However, the pH of the premixed MTAs increased with time. TLC released a higher concentration of strontium ion compared with the other materials. Meanwhile, EMPR released a significantly high concentration of sulfide ion (p < 0.05). In the after-setting group, the 2 kinds of premixed MTAs released a significantly higher concentration of calcium ion compared with the other materials (p < 0.05). In the after-setting group, EMPR had a significantly low solubility value (p < 0.05). The Kruskal-Wallis test, followed by the Mann-Whitney U test with Bonferroni correction, was used in statistical analysis. In conclusion, resin-modified calcium silicate cement, modified calcium hydroxide cement, and the 2 kinds of premixed MTAs had an alkaline pH and low solubility value and they released various concentrations of ions after setting.
- Published
- 2022
7. Difference in Bonding Strength of RMGIC according to Type of Hemostatic Agent in Primary Tooth
- Author
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Seolah Back, Joonhaeng Lee, Jongbin Kim, Miran Han, and Jong Soo Kim
- Subjects
stomatognathic diseases ,stomatognathic system - Abstract
The purpose of this study was to compare the effect of the hemostatic agent containing aluminum chloride with hemostatic agent containing ferric sulfate on the shear bond strength of resin-modified glass ionomer cement(RMGIC) to dentin in primary tooth. Twenty extracted non-carious human primary teeth were collected in this study. The specimens were cut to expose dentin and polished. The specimens were randomly seperated into 3 groups for treatment; group I: polyacrylic acid(PAA), RMGIC; group II: aluminum chloride, PAA, RMGIC; group III: ferric sulfate, PAA, RMGIC Ten specimens from each group were subjected to shear bond strength test. The mean shear bond strength of each group was as follows: 10.07 ± 1.83 MPa in Group I, 7.62 ± 0.78 MPA in group II, 5.23 ± 0.78 MPa in group III. There were significant differences among all groups(p < 0.001). In conclusion, both aluminum chloride hemostatic agent and ferric sulfate hemostatic agent decreased the shear bond strength of RMGIC to dentin. And ferric sulfate hemostatic agent decreased the shear bond strength of RMGIC more than the aluminium chloride hemostatic agent.
- Published
- 2021
8. Effect of Aluminum Chloride Hemostatic Agent on Bonding Strength of RMGIC in Primary Tooth
- Author
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Seung-Hee Woo, Joonhaeng Lee, Jong-Soo Kim, Miran Han, and Jisun Shin
- Subjects
Hemostatic Agent ,Materials science ,chemistry.chemical_element ,Chloride ,Hemostatics ,stomatognathic diseases ,stomatognathic system ,chemistry ,Bonding strength ,Aluminium ,medicine ,Primary Tooth ,Composite material ,medicine.drug - Abstract
The purpose of this study was to evaluate the effect of a hemostatic agent containing aluminum chloride on the shear bond strength of resin-modified glass ionomer cement (RMGIC) to the dentin of primary teeth. Thirty-six extracted non-carious human primary teeth were collected in this study. Dentin surfaces were cut and polished. The specimens were randomly divided into 4 groups; group I: RMGIC without conditioning; group II: polyacrylic acid (PAA), RMGIC; group III: aluminum chloride, RMGIC; group IV: aluminum chloride, PAA, RMGIC. All teeth were thermocycled between 5.0℃ and 55.0℃ for 5000 cycles. Fifteen specimens from each group were subjected to shear bond strength test and 3 specimens from each group were inspected using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy. The mean shear bond strength of each group was as follows: 4.04 ± 0.88 MPa in group I, 8.29 ± 1.40 MPa in group II, 1.39 ± 0.47 MPa in group III, 6.24 ± 2.76 MPa in group IV. There were significant differences among all groups (p < 0.001). SEM image of the dentinal tubules were partially exposed in group III and group IV. Fully exposed dentinal tubules were found in group II. In conclusion, aluminum chloride decreased the shear bond strength of RMGIC to dentin, regardless of PAA conditioning.
- Published
- 2021
9. Effect of Sodium Fluoride Varnish and Potassium Iodide on Remineralization Efficacy of Silver Diamine Fluoride
- Author
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Jisun Shin, Junyong Ahn, Jong-Soo Kim, Kunho Lee, Joonhaeng Lee, and Miran Han
- Subjects
Remineralisation ,chemistry.chemical_compound ,Chemistry ,visual_art ,Varnish ,Sodium fluoride ,visual_art.visual_art_medium ,chemistry.chemical_element ,Silver diamine fluoride ,Iodine ,Nuclear chemistry - Abstract
The purpose of this study was to compare the effect of sodium fluoride(NaF) varnish and potassium iodide(KI) on remineralization efficacy of silver diamine fluoride(SDF) by measuring microhardness and evaluating surface morphology by scanning electron microscope(SEM). Artificial caries lesions were induced on extracted primary molars and vickers microhardness was measured. Specimens were randomly separated into 4 groups for treatment. The specimens in group I were treated with SDF, group II with NaF varnish after SDF, group III with KI after SDF and group IV with distilled water. After 8 days of pH cycling, vickers microhardness was measured and difference before and after treatment was calculated. For SEM, 2 samples were evaluated respectively after enamel polishing, lesion formation and after pH cycling. Group III showed highest increase in microhardness. Group I showed higher increase in microhardness than Group II but without statistical difference. Group IV showed lowest increase in microhardness value among 4 groups. On SEM image, group I, II and III showed smoother and less irregular surface compared to group IV. Amorphous crystal pellicles were observed in group III. In conclusion, SDF, SDF and NaF, SDF and KI groups showed smoother surface and increase in microhardness suggesting the possibility that remineralization effect might take place in oral conditions. In addition, in limited conditions of this study, applying NaF varnish after SDF did not increase the remineralization efficacy of SDF while KI significantly increased the remineralization efficacy of SDF. However, additional study considering various conditions that might affect demineralization and remineralization in clinical situations need to be conducted.
- Published
- 2021
10. Characterization of Odontoblasts in Supernumerary Tooth-derived Dental Pulp Stem Cells between Passages by Real-Time PCR
- Author
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Joonhaeng Lee, Jongbin Kim, Sol Song, Sang-Eun Ji, and Jong-Soo Kim
- Subjects
stomatognathic diseases ,Odontoblast ,Real-time polymerase chain reaction ,Supernumerary tooth ,stomatognathic system ,Dental pulp stem cells ,Biology ,Molecular biology - Abstract
The aim of this study is to compare the properties of odontoblast gene of early passage cells and late passage cells derived from impacted maxillary supernumerary teeth. Impacted supernumerary teeth with maxilla were extracted from 12 patients (8 males, 4 females) between 6 - 9 years old without medical history. Real-time polymerase chain reaction (PCR) was conducted to compare characterization of odontoblast cell in the 3rd and 10th passage, and between with bone inducing additive group and without additive group. Genes for odontoblasts characteristics are osteonectin (ONT), alkaline phosphatase (ALP), osteocalcin (OCN), dentin matrix protein 1 (DMP-1) and dentin sialophosphoprotein (DSPP). The level of gene expression was in a decreasing order of ONT, ALP, OCN, DMP-1 and DSPP in the 3rd passage, and in decreasing order of ONT, DMP-1, OCN, ALP, and DSPP in the 10th passage in the undifferentiation and differentiation group. The order of ONT, DMP-1, and OCN did not changed. ALP and DMP-1 were switched in order. ALP and DMP-1 may be used as important markers for differentiating between the 3rd passage and 10th passage cells. Considering that supernumerary tooth was extracted young age and the time required to cultured 10th passage was short, supernumerary tooth can be considered a useful donor site of dental pulp stem cells.
- Published
- 2021
11. Antibacterial Effect on Enterococcus Faecalis and Physical Properties of Chitosan Added Calcium Hydroxide Canal Filling Material
- Author
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Sol Song, Joonhaeng Lee, Jisun Shin, Jongbin Kim, Jung-Hwan Lee, and Yu-Jin Kim
- Subjects
0303 health sciences ,Calcium hydroxide ,biology ,Radiodensity ,technology, industry, and agriculture ,macromolecular substances ,030206 dentistry ,Antibacterial effect ,biology.organism_classification ,Enterococcus faecalis ,carbohydrates (lipids) ,Chitosan ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,chemistry ,030304 developmental biology ,Nuclear chemistry - Abstract
The aim of this study was to evaluate the antibacterial effect on Enterococcus Faecalis and physical properties of chitosan added calcium hydroxide canal filling material.Low, medium, high molecular weights of chitosan powder were mixed with calcium hydroxide canal filling material. Also, for each molecular weight group, 1.0, 2.0, 5.0 wt% of chitosan powder were added. An overnight culture of E. faecalis was adjusted to 1 × 106 CFU/ml. For test of antibacterial effect, three different molecular weights of 2.0 wt% chitosan and three different concentrations of high molecular weight chitosan were mixed with calcium hydroxide canal filling material. The absorbance of plates was analyzed using spectrophotometer at 570 nm with a reference wavelength of 600 nm. Physical properties such as flow, film thickness and radiopacity were examined according to ISO 6876 : 2012.All molecular weight type of chitosan containing material showed inhibitory effect against E. faecalis growth compared to non-chitosan added calcium hydroxide canal filling material group (p < 0.05). High molecular weight chitosan containing material showed the most antibacterial effect. Also, the antibacterial effect decreased as the incorporated amount of chitosan decreased (p < 0.05). Every molecular weight group of material containing chitosan had a tendency for reduced flow and radiopacity, increased film thickness according to amount of chitosan. Low molecular weight of 1.0 wt% chitosan addition did not show any significant difference of physical properties compared to conventional calcium hydroxide canal filling material.In conclusion, for reinforcement of antibacterial effect against E. faecalis and for favorable physical properties, 2.0 wt% of chitosan adding is recommended. Considering its antibacterial effect of chitosan, further studies are required for clinical application of chitosan in endodontics and pediatric dentistry.
- Published
- 2021
12. Benzocaine, Methemoglobinemia and Methylene Blue
- Author
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Jongbin Kim and Joonhaeng Lee
- Subjects
Side effect ,business.industry ,030206 dentistry ,Methemoglobinemia ,medicine.disease ,Medicinal chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Benzocaine ,0302 clinical medicine ,chemistry ,Medicine ,030212 general & internal medicine ,business ,Methylene blue ,medicine.drug - Published
- 2018
13. Evaluation of the Reliability of Salivary Testing Instrument
- Author
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Joonhaeng Lee and Keumah Han
- Subjects
03 medical and health sciences ,0302 clinical medicine ,business.industry ,Medicine ,030209 endocrinology & metabolism ,030206 dentistry ,business ,Reliability (statistics) ,Reliability engineering - Published
- 2018
14. High-Efficiency Gene Transfer into Rhesus Macaque Primary T Lymphocytes by Combining 32°C Centrifugation and CH-296-Coated Plates: Effect of Gene Transfer Protocol on T Cell Homing Receptor Expression
- Author
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Patina Moore, Joonhaeng Lee, Kathleen M. Brasky, and Paul Zhou
- Subjects
Integrins ,Time Factors ,CD3 Complex ,T-Lymphocytes ,viruses ,Receptor expression ,Cell Separation ,Recombinant virus ,medicine.disease_cause ,Polymerase Chain Reaction ,Jurkat Cells ,Transduction, Genetic ,Lymphocytes ,L-Selectin ,Cells, Cultured ,Genetic transfer ,Gene Transfer Techniques ,Temperature ,Flow Cytometry ,Leukemia Virus, Murine ,Rhesus macaque ,medicine.anatomical_structure ,CD4 Antigens ,Molecular Medicine ,CD8 Antigens ,T cell ,Genetic Vectors ,Green Fluorescent Proteins ,Receptors, Antigen, T-Cell ,Biology ,Cell Line ,CD28 Antigens ,Genetics ,medicine ,Animals ,Humans ,Molecular Biology ,Dose-Response Relationship, Drug ,T lymphocyte ,Simian immunodeficiency virus ,biology.organism_classification ,Macaca mulatta ,Virology ,Molecular biology ,Culture Media ,Fibronectins ,Luminescent Proteins ,Retroviridae ,Ex vivo ,HeLa Cells - Abstract
Although steady progress has been made in transducing human T lymphocytes by Moloney murine leukemia virus (Mo-MuLV)-based vectors, few studies have been done to define ex vivo gene transfer protocols to transduce rhesus macaque primary T lymphocytes. Given the fact that simian immunodeficiency virus (SIV) infection in rhesus macaque is a well-characterized model for human immunodeficiency virus (HIV), it is of great interest to develop an efficient protocol to transduce rhesus macaque primary T cells. In this study, we have used MuLV-10A1-pseudotyped retrovirus expressing enhanced green fluorescent protein (EGFP) to evaluate a number of ex vivo gene transfer protocols in rhesus macaque primary T lymphocytes. Our objectives in designing these protocols were (1) to test whether higher efficiency gene transfer could be obtained by combining two previously defined protocols, centrifugation at 32 degrees C and the CH-296-coated plate; and (2) to study the effect of an ex vivo gene transfer protocol on the expression of lymphocyte homing receptors L-selectin and alpha 4 beta 7 and alpha 4 beta 1 integrins. From seven independent experiments we demonstrate by flow cytometry analyses of EGFP expression that whereas centrifugation at 32 degrees C or the fibronectin fragment CH-296-coated plate protocol alone yielded 10-14% transduction efficiency, combining these two protocols resulted in 28.1-51.2% transduction efficiency. EGFP in transduced cells was expressed highly throughout the 14 days of posttransduction expansion. Our results also demonstrate that whereas the transduction procedure per se did not significantly alter the expression of lymphocyte homing receptors, anti-CD3 and anti-CD28 antibody stimulation profoundly reduced the expression of L-selectin. The selective reduction of L-selectin may result in significant in vivo consequences if transduced cells are infused.
- Published
- 2001
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