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1. Antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) abundance in animal faeces, meat, production environments and human faeces in Europe

Author

Yang, Dongsheng, Heederik, Dick J J, Scherpenisse, Peter, Van Gompel, Liese, Luiken, Roosmarijn E C, Wadepohl, Katharina, Skarżyńska, Magdalena, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Portengen, Lützen, Juraschek, Katharina, Fischer, Jennie, Zając, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Mevius, Dik J, Smit, Lidwien A M, Schmitt, Heike, Yang, Dongsheng, Heederik, Dick J J, Scherpenisse, Peter, Van Gompel, Liese, Luiken, Roosmarijn E C, Wadepohl, Katharina, Skarżyńska, Magdalena, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Portengen, Lützen, Juraschek, Katharina, Fischer, Jennie, Zając, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Mevius, Dik J, Smit, Lidwien A M, and Schmitt, Heike more...

Published
2022

2. Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries

Author

Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, Schmitt, Heike, Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, and Schmitt, Heike more...

Abstract

OBJECTIVES: The occurrence and zoonotic potential of antimicrobial resistance (AMR) in pigs and broilers has been studied intensively in past decades. Here, we describe AMR levels of European pig and broiler farms and determine the potential risk factors.METHODS: We collected faeces from 181 pig farms and 181 broiler farms in nine European countries. Real-time quantitative PCR (qPCR) was used to quantify the relative abundance of four antimicrobial resistance genes (ARGs) [aph(3')-III, erm(B), sul2 and tet(W)] in these faeces samples. Information on antimicrobial use (AMU) and other farm characteristics was collected through a questionnaire. A mixed model using country and farm as random effects was performed to evaluate the relationship of AMR with AMU and other farm characteristics. The correlation between individual qPCR data and previously published pooled metagenomic data was evaluated. Variance component analysis was conducted to assess the variance contribution of all factors.RESULTS: The highest abundance of ARG was for tet(W) in pig faeces and erm(B) in broiler faeces. In addition to the significant positive association between corresponding ARG and AMU levels, we also found on-farm biosecurity measures were associated with relative ARG abundance in both pigs and broilers. Between-country and between-farm variation can partially be explained by AMU. Different ARG targets may have different sample size requirements to represent the overall farm level precisely.CONCLUSIONS: qPCR is an efficient tool for targeted assessment of AMR in livestock-related samples. The AMR variation between samples was mainly contributed to by between-country, between-farm and within-farm differences, and then by on-farm AMU. more...

Published
2022

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3. Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries

Author

IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, dIRAS RA-I&I RA, Klinische infectiologie en microb. lab., dI&I I&I-4, dIRAS RA-I&I I&I, LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, Schmitt, Heike, IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, dIRAS RA-I&I RA, Klinische infectiologie en microb. lab., dI&I I&I-4, dIRAS RA-I&I I&I, LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, and Schmitt, Heike more...

Published
2022

4. Antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) abundance in animal faeces, meat, production environments and human faeces in Europe

Author

IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, dIRAS RA-I&I RA, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, IRAS OH Epidemiology Chemical Agents, dIRAS RA-2, dI&I I&I-4, Yang, Dongsheng, Heederik, Dick J J, Scherpenisse, Peter, Van Gompel, Liese, Luiken, Roosmarijn E C, Wadepohl, Katharina, Skarżyńska, Magdalena, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Portengen, Lützen, Juraschek, Katharina, Fischer, Jennie, Zając, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Mevius, Dik J, Smit, Lidwien A M, Schmitt, Heike, IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, dIRAS RA-I&I RA, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, IRAS OH Epidemiology Chemical Agents, dIRAS RA-2, dI&I I&I-4, Yang, Dongsheng, Heederik, Dick J J, Scherpenisse, Peter, Van Gompel, Liese, Luiken, Roosmarijn E C, Wadepohl, Katharina, Skarżyńska, Magdalena, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Portengen, Lützen, Juraschek, Katharina, Fischer, Jennie, Zając, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Mevius, Dik J, Smit, Lidwien A M, and Schmitt, Heike more...

Published
2022

5. Antimicrobial resistance genes aph(3′)-III, erm(B), sul2 and tet(W) abundance in animal faeces, meat, production environments and human faeces in Europe

Author

Yang, Dongsheng, primary, Heederik, Dick J J, additional, Scherpenisse, Peter, additional, Van Gompel, Liese, additional, Luiken, Roosmarijn E C, additional, Wadepohl, Katharina, additional, Skarżyńska, Magdalena, additional, Van Heijnsbergen, Eri, additional, Wouters, Inge M, additional, Greve, Gerdit D, additional, Jongerius-Gortemaker, Betty G M, additional, Tersteeg-Zijderveld, Monique, additional, Portengen, Lützen, additional, Juraschek, Katharina, additional, Fischer, Jennie, additional, Zając, Magdalena, additional, Wasyl, Dariusz, additional, Wagenaar, Jaap A, additional, Mevius, Dik J, additional, Smit, Lidwien A M, additional, and Schmitt, Heike, additional more...

Published
2022
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6. Association of antimicrobial usage with faecal abundance of aph(3')-III, ermB, sul2 and tetW resistance genes in veal calves in three European countries

Author

Yang, Dongsheng, Van Gompel, Liese, Luiken, Roosmarijn E C, Sanders, Pim, Joosten, Philip, van Heijnsbergen, Eri, Wouters, Inge M, Scherpenisse, Peter, Chauvin, Claire, Wadepohl, Katharina, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Soumet, Christophe, Skarżyńska, Magdalena, Juraschek, Katharina, Fischer, Jennie, Wasyl, Dariusz, Wagenaar, Jaap A, Dewulf, Jeroen, Schmitt, Heike, Mevius, Dik J, Heederik, Dick J J, Smit, Lidwien A M, IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, One Health Microbieel, dI&I I&I-4, Faculteit Diergeneeskunde, IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, One Health Microbieel, dI&I I&I-4, Faculteit Diergeneeskunde, Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and Laboratoire de Fougères - ANSES more...

Subjects
0301 basic medicine, Veterinary medicine, Epidemiology, animal diseases, Resistance genes, ruminant, Antimicrobial resistance, Trimethoprim, 0302 clinical medicine, Abundance (ecology), Germany, Surveys and Questionnaires, fécès, Pharmacology (medical), AMR, 030212 general & internal medicine, bacteria, résistance aux antimicrobiels, Netherlands, 2. Zero hunger, bactérie, Sulfonamides, AMU, veau, General Medicine, veal, Antimicrobial, Anti-Bacterial Agents, Europe, Drug Combinations, qPCR, Infectious Diseases, Antimicrobial resistance genes, France, medicine.drug, Microbiology (medical), Bioinformatica & Diermodellen, 030106 microbiology, Coronacrisis-Taverne, Cattle Diseases, Biology, Real-Time Polymerase Chain Reaction, 03 medical and health sciences, Antibiotic resistance, Bacterial Proteins, Negatively associated, Bio-informatics & Animal models, medicine, Animals, Epidemiology, Bio-informatics & Animal models, gene, Prescription Drug Overuse, Feces, Epidemiologie, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Veal calves, Kanamycin Kinase, gène, association, Methyltransferases, antimicrobial use, usage des antimicrobiens, Antimicrobial use, feces, Risk factors, cattle, Epidemiologie, Bioinformatica & Diermodellen, Carrier Proteins
Abstract

International audience; Background High antimicrobial use (AMU) and antimicrobial resistance (AMR) in veal calves remain a source of concern. As part of the EFFORT project, the association between AMU and the abundance of faecal antimicrobial resistance genes (ARGs) in veal calves in three European countries was determined. Methods In 2015, faecal samples of veal calves close to slaughter were collected from farms located in France, Germany and the Netherlands (20 farms in France, 20 farms in the Netherlands and 21 farms in Germany; 25 calves per farm). Standardized questionnaires were used to record AMU and farm characteristics. In total, 405 faecal samples were selected for DNA extraction and quantitative polymerase chain reaction to quantify the abundance (16S normalized concentration) of four ARGs [aph(3’)-III, ermB, sul2 and tetW] encoding for resistance to frequently used antimicrobials in veal calves. Multiple linear mixed models with random effects for country and farm were used to relate ARGs to AMU and farm characteristics. Results A significant positive association was found between the use of trimethoprim/sulfonamides and the concentration of sul2 in faeces from veal calves. A higher weight of calves on arrival at the farm was negatively associated with aph(3’)-III and ermB. Lower concentrations of aph(3’)-III were found at farms with non-commercial animals present. Furthermore, farms using only water for the cleaning of stables had a significantly lower abundance of faecal ermB and tetW compared with other farms. Conclusion A positive association was found between the use of trimethoprim/sulfonamides and the abundance of sul2 in faeces in veal calves. Additionally, other relevant risk factors associated with ARGs in veal calves were identified, such as weight on arrival at the farm and cleaning practices. more...

Published
2020
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7. Description and determinants of the faecal resistome and microbiome of farmers and slaughterhouse workers: A metagenome-wide cross-sectional study

Author

Van Gompel, Liese, Luiken, Roosmarijn E C, Hansen, Rasmus B, Munk, Patrick, Bouwknegt, Martijn, Heres, Lourens, Greve, Gerdit D, Scherpenisse, Peter, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique H G, García-Cobos, Silvia, Dohmen, Wietske, Dorado-García, Alejandro, Wagenaar, Jaap A, Urlings, Bert A P, Aarestrup, Frank M, Mevius, Dik J, Heederik, Dick J J, Schmitt, Heike, Bossers, Alex, Smit, Lidwien A M, Van Gompel, Liese, Luiken, Roosmarijn E C, Hansen, Rasmus B, Munk, Patrick, Bouwknegt, Martijn, Heres, Lourens, Greve, Gerdit D, Scherpenisse, Peter, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique H G, García-Cobos, Silvia, Dohmen, Wietske, Dorado-García, Alejandro, Wagenaar, Jaap A, Urlings, Bert A P, Aarestrup, Frank M, Mevius, Dik J, Heederik, Dick J J, Schmitt, Heike, Bossers, Alex, and Smit, Lidwien A M more...

Published
2020

8. Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers

Author

Van Gompel, Liese, Dohmen, Wietske, Luiken, Roosmarijn E C, Bouwknegt, Martijn, Heres, Lourens, Van Heijnsbergen, Eri, Jongerius-Gortemaker, Betty G M, Scherpenisse, Peter, Greve, Gerdit D, Tersteeg-Zijderveld, Monique H G, Wadepohl, Katharina, Ribeiro Duarte, Ana Sofia, Muñoz-Gómez, Violeta, Fischer, Jennie, Skarżyńska, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Urlings, Bert A P, Dorado-García, Alejandro, Wouters, Inge M, Heederik, Dick J J, Schmitt, Heike, Smit, Lidwien A M, Van Gompel, Liese, Dohmen, Wietske, Luiken, Roosmarijn E C, Bouwknegt, Martijn, Heres, Lourens, Van Heijnsbergen, Eri, Jongerius-Gortemaker, Betty G M, Scherpenisse, Peter, Greve, Gerdit D, Tersteeg-Zijderveld, Monique H G, Wadepohl, Katharina, Ribeiro Duarte, Ana Sofia, Muñoz-Gómez, Violeta, Fischer, Jennie, Skarżyńska, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Urlings, Bert A P, Dorado-García, Alejandro, Wouters, Inge M, Heederik, Dick J J, Schmitt, Heike, and Smit, Lidwien A M more...

Abstract

OBJECTIVES: Slaughterhouse staff is occupationally exposed to antimicrobial resistant bacteria. Studies reported high antimicrobial resistance gene (ARG) abundances in slaughter pigs. This cross-sectional study investigated occupational exposure to tetracycline (tetW) and macrolide (ermB) resistance genes and assessed determinants for faecal tetW and ermB carriage among pig slaughterhouse workers.METHODS: During 2015-2016, 483 faecal samples and personal questionnaires were collected from workers in a Dutch pig abattoir, together with 60 pig faecal samples. Human dermal and respiratory exposure was assessed by examining 198 carcass, 326 gloves, and 33 air samples along the line, next to 198 packed pork chops to indicate potential consumer exposure. Samples were analyzed by qPCR (tetW, ermB). A job exposure matrix was created by calculating the percentage of tetW and ermB positive carcasses or gloves for each job position. Multiple linear regression models were used to link exposure to tetW and ermB carriage.RESULTS: Workers are exposed to tetracycline and macrolide resistance genes along the slaughter line. Tetw and ermB gradients were found for carcasses, gloves, and air filters. One packed pork chop contained tetW, ermB was non-detectable. Human faecal tetW and ermB concentrations were lower than in pig faeces. Associations were found between occupational tetW exposure and human faecal tetW carriage, yet, not after model adjustments. Sampling round, nationality, and smoking were determinants for ARG carriage.CONCLUSION: We demonstrated clear environmental tetracycline and macrolide resistance gene exposure gradients along the slaughter line. No robust link was found between ARG exposure and human faecal ARG carriage. more...

Published
2020

9. Description and determinants of the faecal resistome and microbiome of farmers and slaughterhouse workers: A metagenome-wide cross-sectional study

Author

IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., One Health Microbieel, dIRAS RA-I&I RA, dI&I I&I-4, Faculteit Diergeneeskunde, Van Gompel, Liese, Luiken, Roosmarijn E C, Hansen, Rasmus B, Munk, Patrick, Bouwknegt, Martijn, Heres, Lourens, Greve, Gerdit D, Scherpenisse, Peter, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique H G, García-Cobos, Silvia, Dohmen, Wietske, Dorado-García, Alejandro, Wagenaar, Jaap A, Urlings, Bert A P, Aarestrup, Frank M, Mevius, Dik J, Heederik, Dick J J, Schmitt, Heike, Bossers, Alex, Smit, Lidwien A M, IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., One Health Microbieel, dIRAS RA-I&I RA, dI&I I&I-4, Faculteit Diergeneeskunde, Van Gompel, Liese, Luiken, Roosmarijn E C, Hansen, Rasmus B, Munk, Patrick, Bouwknegt, Martijn, Heres, Lourens, Greve, Gerdit D, Scherpenisse, Peter, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique H G, García-Cobos, Silvia, Dohmen, Wietske, Dorado-García, Alejandro, Wagenaar, Jaap A, Urlings, Bert A P, Aarestrup, Frank M, Mevius, Dik J, Heederik, Dick J J, Schmitt, Heike, Bossers, Alex, and Smit, Lidwien A M more...

Published
2020

10. Association of antimicrobial usage with faecal abundance of aph(3')-III, ermB, sul2 and tetW resistance genes in veal calves in three European countries

Author

IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, One Health Microbieel, dI&I I&I-4, Faculteit Diergeneeskunde, Yang, Dongsheng, Van Gompel, Liese, Luiken, Roosmarijn E C, Sanders, Pim, Joosten, Philip, van Heijnsbergen, Eri, Wouters, Inge M, Scherpenisse, Peter, Chauvin, Claire, Wadepohl, Katharina, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Soumet, Christophe, Skarżyńska, Magdalena, Juraschek, Katharina, Fischer, Jennie, Wasyl, Dariusz, Wagenaar, Jaap A, Dewulf, Jeroen, Schmitt, Heike, Mevius, Dik J, Heederik, Dick J J, Smit, Lidwien A M, IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, One Health Microbieel, dI&I I&I-4, Faculteit Diergeneeskunde, Yang, Dongsheng, Van Gompel, Liese, Luiken, Roosmarijn E C, Sanders, Pim, Joosten, Philip, van Heijnsbergen, Eri, Wouters, Inge M, Scherpenisse, Peter, Chauvin, Claire, Wadepohl, Katharina, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Soumet, Christophe, Skarżyńska, Magdalena, Juraschek, Katharina, Fischer, Jennie, Wasyl, Dariusz, Wagenaar, Jaap A, Dewulf, Jeroen, Schmitt, Heike, Mevius, Dik J, Heederik, Dick J J, and Smit, Lidwien A M more...

Published
2020

11. Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers

Author

One Health Microbieel, dIRAS RA-I&I I&I, LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, LS Klinisch Onderzoek Wagenaar, dI&I I&I-4, Faculteit Diergeneeskunde, Van Gompel, Liese, Dohmen, Wietske, Luiken, Roosmarijn E C, Bouwknegt, Martijn, Heres, Lourens, Van Heijnsbergen, Eri, Jongerius-Gortemaker, Betty G M, Scherpenisse, Peter, Greve, Gerdit D, Tersteeg-Zijderveld, Monique H G, Wadepohl, Katharina, Ribeiro Duarte, Ana Sofia, Muñoz-Gómez, Violeta, Fischer, Jennie, Skarżyńska, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Urlings, Bert A P, Dorado-García, Alejandro, Wouters, Inge M, Heederik, Dick J J, Schmitt, Heike, Smit, Lidwien A M, One Health Microbieel, dIRAS RA-I&I I&I, LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, LS Klinisch Onderzoek Wagenaar, dI&I I&I-4, Faculteit Diergeneeskunde, Van Gompel, Liese, Dohmen, Wietske, Luiken, Roosmarijn E C, Bouwknegt, Martijn, Heres, Lourens, Van Heijnsbergen, Eri, Jongerius-Gortemaker, Betty G M, Scherpenisse, Peter, Greve, Gerdit D, Tersteeg-Zijderveld, Monique H G, Wadepohl, Katharina, Ribeiro Duarte, Ana Sofia, Muñoz-Gómez, Violeta, Fischer, Jennie, Skarżyńska, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Urlings, Bert A P, Dorado-García, Alejandro, Wouters, Inge M, Heederik, Dick J J, Schmitt, Heike, and Smit, Lidwien A M more...

Published
2020

12. Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers

Author

Van Gompel, Liese, primary, Dohmen, Wietske, additional, Luiken, Roosmarijn E C, additional, Bouwknegt, Martijn, additional, Heres, Lourens, additional, van Heijnsbergen, Eri, additional, Jongerius-Gortemaker, Betty G M, additional, Scherpenisse, Peter, additional, Greve, Gerdit D, additional, Tersteeg-Zijderveld, Monique H G, additional, Wadepohl, Katharina, additional, Ribeiro Duarte, Ana Sofia, additional, Muñoz-Gómez, Violeta, additional, Fischer, Jennie, additional, Skarżyńska, Magdalena, additional, Wasyl, Dariusz, additional, Wagenaar, Jaap A, additional, Urlings, Bert A P, additional, Dorado-García, Alejandro, additional, Wouters, Inge M, additional, Heederik, Dick J J, additional, Schmitt, Heike, additional, and Smit, Lidwien A M, additional more...

Published
2019
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13. Cinnamaldehyde, Carvacrol and Organic Acids Affect Gene Expression of Selected Oxidative Stress and Inflammation Markers in IPEC-J2 Cells Exposed to Salmonella typhimurium

Author

Burt, Sara A, Adolfse, Simone J M, Ahad, Dina S A, Tersteeg-Zijderveld, Monique H G, Jongerius-Gortemaker, Betty G M, Post, Jan A, Brüggemann, Holger, Santos, Regiane R, LS IRAS VPH VV (veterinaire volksgezh.), Sub Cell Biology, dIRAS RA-I&I RA, LS IRAS VPH VV (veterinaire volksgezh.), Sub Cell Biology, and dIRAS RA-I&I RA more...

Subjects
0301 basic medicine, Salmonella typhimurium, Salmonella, 030106 microbiology, Fimbria, Biology, medicine.disease_cause, Cinnamaldehyde, Cinnamic acid, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Minimum inhibitory concentration, medicine, Animals, Carvacrol, Viability assay, Acrolein, essential oils, Research Articles, Pharmacology, Epithelial Cells, IPEC-J2, Oxidative Stress, 030104 developmental biology, chemistry, Biochemistry, inflammation, IPEC‐J2, Monoterpenes, gene expression, Cymenes, Oxidative stress, Research Article
Abstract

Essential oils and organic acids are used as feed additives to improve health status and reduce colonization with pathogens. Although bactericidal in vitro, concentrations achieved in the animal gut are probably not lethal to pathogens. The aim of this study was to investigate the effects of cinnamaldehyde, carvacrol and cinnamic, lactic and propionic acids on the ability of Salmonella typhimurium ATCC 14028 (ST) to invade intestinal epithelial cells (IPEC-J2) and on the expression levels of immune related genes in the cells. The minimum inhibitory concentration (MIC) and non-inhibitory concentration (NIC) were determined and influence on the invasion capacity of ST was investigated. The structure of fimbriae and flagella was analysed by electron microscopy, and expression levels of HSP70, IkBa, IL-8 and IL-10 in the IPEC-J2 cells were carried out by q-PCR. Cinnamaldehyde, carvacrol and cinnamic and propionic acids inhibited ST invasion but not cell viability, bacterial viability and motility or the development of flagella. Propionic acid and cinnamaldehyde in combination with cinnamic acid caused structural impairment of fimbriae. Cinnamaldehyde up-regulated expression of HSP70 irrespective of the presence of organic acids or ST; exposure to carvacrol induced HSP70 only in the presence of propionic acid and ST. © 2016 The Authors. Phytotherapy Research published by John Wiley & Sons Ltd. more...

Published
2016
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14. Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers.

Author

Gompel, Liese Van, Dohmen, Wietske, Luiken, Roosmarijn E C, Bouwknegt, Martijn, Heres, Lourens, Heijnsbergen, Eri van, Jongerius-Gortemaker, Betty G M, Scherpenisse, Peter, Greve, Gerdit D, Tersteeg-Zijderveld, Monique H G, Wadepohl, Katharina, Duarte, Ana Sofia Ribeiro, Muñoz-Gómez, Violeta, Fischer, Jennie, Skarżyńska, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Urlings, Bert A P, Dorado-García, Alejandro, and Wouters, Inge M more...

Subjects
FECAL analysis, DRUG resistance in microorganisms, FOOD industry, GENES, MACROLIDE antibiotics, POLYMERASE chain reaction, QUESTIONNAIRES, SWINE, TETRACYCLINE, OCCUPATIONAL hazards, ENVIRONMENTAL exposure, MULTIPLE regression analysis, CROSS-sectional method
Abstract

Objectives Slaughterhouse staff is occupationally exposed to antimicrobial resistant bacteria. Studies reported high antimicrobial resistance gene (ARG) abundances in slaughter pigs. This cross-sectional study investigated occupational exposure to tetracycline (tetW) and macrolide (ermB) resistance genes and assessed determinants for faecal tetW and ermB carriage among pig slaughterhouse workers. Methods During 2015–2016, 483 faecal samples and personal questionnaires were collected from workers in a Dutch pig abattoir, together with 60 pig faecal samples. Human dermal and respiratory exposure was assessed by examining 198 carcass, 326 gloves, and 33 air samples along the line, next to 198 packed pork chops to indicate potential consumer exposure. Samples were analyzed by qPCR (tetW , ermB). A job exposure matrix was created by calculating the percentage of tetW and ermB positive carcasses or gloves for each job position. Multiple linear regression models were used to link exposure to tetW and ermB carriage. Results Workers are exposed to tetracycline and macrolide resistance genes along the slaughter line. Tetw and ermB gradients were found for carcasses, gloves, and air filters. One packed pork chop contained tetW , ermB was non-detectable. Human faecal tetW and ermB concentrations were lower than in pig faeces. Associations were found between occupational tetW exposure and human faecal tetW carriage, yet, not after model adjustments. Sampling round, nationality, and smoking were determinants for ARG carriage. Conclusion We demonstrated clear environmental tetracycline and macrolide resistance gene exposure gradients along the slaughter line. No robust link was found between ARG exposure and human faecal ARG carriage. [ABSTRACT FROM AUTHOR] more...

Published
2020
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15. Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries.

Author

Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, Schmitt, Heike, Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, and Schmitt, Heike more...

16. Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries.

Author

Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, Schmitt, Heike, Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, and Schmitt, Heike more...

17. Description and determinants of the faecal resistome and microbiome of farmers and slaughterhouse workers: A metagenome-wide cross-sectional study.

Author

Van Gompel L, Luiken REC, Hansen RB, Munk P, Bouwknegt M, Heres L, Greve GD, Scherpenisse P, Jongerius-Gortemaker BGM, Tersteeg-Zijderveld MHG, García-Cobos S, Dohmen W, Dorado-García A, Wagenaar JA, Urlings BAP, Aarestrup FM, Mevius DJ, Heederik DJJ, Schmitt H, Bossers A, and Smit LAM more...

Subjects
Abattoirs, Animals, Anti-Bacterial Agents pharmacology, Chickens, Cross-Sectional Studies, Drug Resistance, Bacterial, Farmers, Humans, Macrolides, Netherlands, Swine, Metagenome, Microbiota
Abstract

Background: By studying the entire human faecal resistome and associated microbiome, the diversity and abundance of faecal antimicrobial resistance genes (ARGs) can be comprehensively characterized. Prior culture-based studies have shown associations between occupational exposure to livestock and carriage of specific antimicrobial resistant bacteria. Using shotgun metagenomics, the present study investigated 194 faecal resistomes and bacteriomes from humans occupationally exposed to ARGs in livestock (i.e. pig and poultry farmers, employees and family members and pig slaughterhouse workers) and a control population (Lifelines cohort) in the Netherlands. In addition, we sought to identify determinants for the human resistome and bacteriome composition by applying a combination of multivariate (NMDS, PERMANOVA, SIMPER and DESeq2 analysis) and multivariable regression analysis techniques., Results: Pig slaughterhouse workers and pig farmers carried higher total ARG abundances in their stools compared to broiler farmers and control subjects. Tetracycline, β-lactam and macrolide resistance gene clusters dominated the resistome of all studied groups. No significant resistome alpha diversity differences were found among the four populations. However, the resistome beta diversity showed a separation of the mean resistome composition of pig and pork exposed workers from broiler farmers and controls, independent of their antimicrobial use. We demonstrated differences in resistome composition between slaughter line positions, pig versus poultry exposed workers, as well as differences between farmers and employees versus family members. In addition, we found a significant correlation between the bacteriome and resistome, and significant differences in the bacteriome composition between and within the studied subpopulations. Finally, an in-depth analysis of pig and poultry farms - of which also farm livestock resistomes were analysed - showed positive associations between the number of on-farm working hours and human faecal AMR loads., Conclusion: We found that the total normalized faecal ARG carriage was larger in persons working in the Dutch pork production chain compared to poultry farmers and controls. Additionally, we showed significant differences in resistome and bacteriome composition of pig and pork exposed workers compared to a control group, as well as within-population (farms, slaughterhouse) compositional differences. The number of on-farm working hours and the farm type (pig or broiler) that persons live or work on are determinants for the human faecal resistome. Overall, our results may suggest direct or indirect livestock contact as a determinant for human ARG carriage. Future studies should further focus on the connection between the human and livestock resistome (i.e. transmission routes) to substantiate the evidence for livestock-associated resistome acquisition., (Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.) more...

Published
2020
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18. Association of antimicrobial usage with faecal abundance of aph(3')-III, ermB, sul2 and tetW resistance genes in veal calves in three European countries.

Author

Yang D, Van Gompel L, Luiken REC, Sanders P, Joosten P, van Heijnsbergen E, Wouters IM, Scherpenisse P, Chauvin C, Wadepohl K, Greve GD, Jongerius-Gortemaker BGM, Tersteeg-Zijderveld MHG, Soumet C, Skarżyńska M, Juraschek K, Fischer J, Wasyl D, Wagenaar JA, Dewulf J, Schmitt H, Mevius DJ, Heederik DJJ, and Smit LAM more...

Subjects
Animals, Anti-Bacterial Agents administration & dosage, Bacterial Proteins genetics, Carrier Proteins genetics, Cattle, Cattle Diseases microbiology, Drug Combinations, Feces microbiology, France, Germany, Kanamycin Kinase genetics, Methyltransferases genetics, Netherlands, Prescription Drug Overuse, Real-Time Polymerase Chain Reaction, Surveys and Questionnaires, Anti-Bacterial Agents pharmacology, Bacteria drug effects, Bacteria genetics, Sulfonamides pharmacology, Trimethoprim pharmacology
Abstract

Background: High antimicrobial use (AMU) and antimicrobial resistance (AMR) in veal calves remain a source of concern. As part of the EFFORT project, the association between AMU and the abundance of faecal antimicrobial resistance genes (ARGs) in veal calves in three European countries was determined., Methods: In 2015, faecal samples of veal calves close to slaughter were collected from farms located in France, Germany and the Netherlands (20 farms in France, 20 farms in the Netherlands and 21 farms in Germany; 25 calves per farm). Standardized questionnaires were used to record AMU and farm characteristics. In total, 405 faecal samples were selected for DNA extraction and quantitative polymerase chain reaction to quantify the abundance (16S normalized concentration) of four ARGs [aph(3')-III, ermB, sul2 and tetW] encoding for resistance to frequently used antimicrobials in veal calves. Multiple linear mixed models with random effects for country and farm were used to relate ARGs to AMU and farm characteristics., Results: A significant positive association was found between the use of trimethoprim/sulfonamides and the concentration of sul2 in faeces from veal calves. A higher weight of calves on arrival at the farm was negatively associated with aph(3')-III and ermB. Lower concentrations of aph(3')-III were found at farms with non-commercial animals present. Furthermore, farms using only water for the cleaning of stables had a significantly lower abundance of faecal ermB and tetW compared with other farms., Conclusion: A positive association was found between the use of trimethoprim/sulfonamides and the abundance of sul2 in faeces in veal calves. Additionally, other relevant risk factors associated with ARGs in veal calves were identified, such as weight on arrival at the farm and cleaning practices., (Copyright © 2020 Elsevier Ltd and International Society of Antimicrobial Chemotherapy. All rights reserved.) more...

Published
2020
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19. Surface plasmon resonance (BIACORE) detection of serum antibodies against Salmonella enteritidis and Salmonella typhimurium.

Author

Jongerius-Gortemaker BG, Goverde RL, van Knapen F, and Bergwerff AA

Subjects
Animals, Antigens, Bacterial immunology, Chickens, Kinetics, Reproducibility of Results, Sensitivity and Specificity, Antibodies, Bacterial blood, Salmonella Infections diagnosis, Salmonella enteritidis immunology, Salmonella typhimurium immunology, Surface Plasmon Resonance methods
Abstract

We have used a surface plasmon resonance biosensor (BIACORE 3000) to detect serum antibodies in chickens having current or recent infections. Three well-defined Salmonella flagellar recombinant DNA antigens reflecting Salmonella enteritidis (H:g,m flagellin) and Salmonella typhimurium (H:i and H:1,2 flagellins) expressed in Escherichia coli were each immobilized in a single flow cell of a biosensor chip. Glutathione-S-transferase was immobilized on the surface of another flow cell to monitor non-specific binding. Sera collected from chickens with no history of Salmonella infection, and from chickens infected with Salmonella serotypes infantis, pullorum, gallinarum were used to test the performance of the system. The sensitivity exhibited to a range up to 900 arbitrary response units (RU) for the most positive S. typhimurium serum at a dilution of 1/40. Sera from Salmonella infantis, Salmonella pullorum and Salmonella gallinarum infected birds gave responses less than the cut-off point, which was determined as the averaged response of sera from specific pathogen-free chickens plus three times the standard deviation. A positive response was obtained when these sera and whole blood were fortified with S. enteritidis and S. typhimurium positive serum. The sensitivity, specificity, precision and reproducibility obtained suggested that this approach could be used for detecting past or present infection with a range of pathogens in animals. more...

Published
2002
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