456 results on '"Jong-Lyel Roh"'
Search Results
2. Unleashing Ferroptosis in Human Cancers: Targeting Ferroptosis Suppressor Protein 1 for Overcoming Therapy Resistance
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Jaewang Lee and Jong-Lyel Roh
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ferroptosis ,ferroptosis suppressor protein 1 ,FSP1 inhibitor ,cancer ,therapy ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Ferroptosis, a recently identified form of regulated cell death characterized by the iron-dependent accumulation of lethal lipid peroxidation, has gained increasing attention in cancer therapy. Ferroptosis suppressor protein 1 (FSP1), an NAD(P)H-ubiquinone oxidoreductase that reduces ubiquinone to ubiquinol, has emerged as a critical player in the regulation of ferroptosis. FSP1 operates independently of the canonical system xc–/glutathione peroxidase 4 pathway, making it a promising target for inducing ferroptosis in cancer cells and overcoming ferroptosis resistance. This review provides a comprehensive overview of FSP1 and ferroptosis, emphasizing the importance of FSP1 modulation and its potential as a therapeutic target in cancer treatment. We also discuss recent progress in developing FSP1 inhibitors and their implications for cancer therapy. Despite the challenges associated with targeting FSP1, advances in this field may provide a strong foundation for developing innovative and effective treatments for cancer and other diseases.
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- 2023
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3. Targeting Iron-Sulfur Clusters in Cancer: Opportunities and Challenges for Ferroptosis-Based Therapy
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Jaewang Lee and Jong-Lyel Roh
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iron ,ferroptosis ,iron-sulfur cluster ,cancer ,therapy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Iron dysregulation is a hallmark of cancer, characterized by an overexpression of genes involved in iron metabolism and iron-sulfur cluster (ISC) biogenesis. Dysregulated iron homeostasis increases intracellular labile iron, which may lead to the formation of excess cytotoxic radicals and make it vulnerable to various types of regulated cell death, including ferroptosis. The inhibition of ISC synthesis triggers the iron starvation response, increasing lipid peroxidation and ferroptosis in cancer cells treated with oxidative stress-inducing agents. Various methods, such as redox operations, iron chelation, and iron replacement with redox-inert metals, can destabilize or limit ISC formation and function, providing potential therapeutic strategies for cancer treatment. Targeting ISCs to induce ferroptosis represents a promising approach in cancer therapy. This review summarizes the state-of-the-art overview of iron metabolism and ferroptosis in cancer cells, the role of ISC modulation in ferroptosis, and the potential of targeting ISCs for ferroptosis induction in cancer therapy. Further research is necessary to develop and validate these strategies in clinical trials for various cancers, which may ultimately lead to the development of novel and effective treatments for cancer patients.
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- 2023
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4. Poly(rC)-binding protein 1 represses ferritinophagy-mediated ferroptosis in head and neck cancer
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Jaewang Lee, Ji Hyeon You, and Jong-Lyel Roh
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Ferroptosis ,poly(rC)-binding protein 1 ,Ferritinophagy ,Iron ,Lipid peroxidation ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
A cytosolic iron chaperone poly(rC)-binding protein 1 (PCBP1) is a multifunctional RNA-binding protein involving gene transcription, RNA regulation, and iron loading to ferritins. PCBP1 is also known to repress autophagy, but the role of PCBP1 in ferritinophagy and ferroptosis remains unrevealed. Therefore, we examined the role of PCBP1 in ferritinophagy-mediated ferroptosis in head and neck cancer (HNC) cells. The effects of system xc– cystine/glutamate antiporter (xCT) inhibitors and PCBP1 gene silencing/overexpression were tested on HNC cell lines and mouse tumor xenograft models. These effects were analyzed by assessing cell viability and death, lipid reactive oxygen species and iron production, lipid, malondialdehyde, mRNA/protein expression, and autophagy flux assays. Interaction between PCBP1 and BECN1 mRNA was also examined by luciferase and RNA-protein pull-down assays. PCBP1 gene silencing increased autophagosome generation and autophagic flux. Conversely, PCBP1 upregulation inhibited autophagy activation via direct binding to the CU-rich elements on the 3′-untranslated region (3′-UTR) of BECN1 mRNA. The internal deletion or mutation of the 3′-UTR F2 region recovered BECN1 mRNA stability repressed by PCBP1, resulting in enhanced ferritinophagy-mediated ferroptosis. Besides, PCBP1 knockdown promoted polyunsaturated fatty acid peroxidation by increasing ALOX15 expression. Further, excess iron accumulation caused mitochondrial dysfunction in PCBP1-suppressed cells. A ferroptosis inducer sulfasalazine significantly suppressed tumor growth in mice with the transplantation of PCBP1-silenced HNC. Our data suggest that the dual functions of PCBP1 repressing BECN1 and ALOX15 mRNAs contribute to attenuating cancer susceptibility to ferroptosis inducers.
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- 2022
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5. Comparison of Squamous Cell Carcinoma of the Tongue between Young and Old Patients
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Gyuheon Choi, Joon Seon Song, Seung-Ho Choi, Soon Yuhl Nam, Sang Yoon Kim, Jong-Lyel Roh, Bu-Kyu Lee, and Kyung-Ja Cho
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mouth neoplasms ,young adult ,smoking ,drinking ,immunohistochemistry ,Pathology ,RB1-214 - Abstract
Background The worldwide incidence of squamous cell carcinoma of the tongue (SCCOT) in young patients has been increasing. We investigated clinicopathologic features of this unique population and compared them with those of SCCOT in the elderly to delineate its pathogenesis. Methods We compared clinicopathological parameters between patients under and over 45 years old. Immunohistochemical assays of estrogen receptor, progesterone receptor, androgen receptor, p53, p16, mdm2, cyclin D1, and glutathione S-transferase P1 were also compared between them. Results Among 189 cases, 51 patients (27.0%) were under 45 years of age. A higher proportion of women was seen in the young group, but was not statistically significant. Smoking and drinking behaviors between age groups were similar. Histopathological and immunohistochemical analysis showed no significant difference by age and sex other than higher histologic grades observed in young patients. Conclusions SCCOT in young adults has similar clinicopathological features to that in the elderly, suggesting that both progress via similar pathogenetic pathways.
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- 2019
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6. SLC7A11 as a Gateway of Metabolic Perturbation and Ferroptosis Vulnerability in Cancer
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Jaewang Lee and Jong-Lyel Roh
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SLC7A11 ,cysteine ,ferroptosis ,redox ,cancer ,Therapeutics. Pharmacology ,RM1-950 - Abstract
SLC7A11 is a cell transmembrane protein composing the light chain of system xc−, transporting extracellular cystine into cells for cysteine production and GSH biosynthesis. SLC7A11 is a critical gateway for redox homeostasis by maintaining the cellular levels of GSH that counter cellular oxidative stress and suppress ferroptosis. SLC7A11 is overexpressed in various human cancers and regulates tumor development, proliferation, metastasis, microenvironment, and treatment resistance. Upregulation of SLC7A11 in cancers is needed to adapt to high oxidative stress microenvironments and maintain cellular redox homeostasis. High basal ROS levels and SLC7A11 dependences in cancer cells render them vulnerable to further oxidative stress. Therefore, cyst(e)ine depletion may be an effective new strategy for cancer treatment. However, the effectiveness of the SLC7A11 inhibitors or cyst(e)inase has been established in many preclinical studies but has not reached the stage of clinical trials for cancer patients. A better understanding of cysteine and SLC7A11 functions regulating and interacting with redox-active proteins and their substrates could be a promising strategy for cancer treatment. Therefore, this review intends to understand the role of cysteine in antioxidant and redox signaling, the regulators of cysteine bioavailability in cancer, the role of SLC7A11 linking cysteine redox signaling in cancer metabolism and targeting SLC7A11 for novel cancer therapeutics.
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- 2022
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7. Guidelines for the Surgical Management of Oral Cancer: Korean Society of Thyroid-Head and Neck Surgery
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Young-Hoon Joo, Jae-Keun Cho, Bon Seok Koo, Minsu Kwon, Seong Keun Kwon, Soon Young Kwon, Min-Su Kim, Jeong Kyu Kim, Heejin Kim, Innchul Nam, Jong-Lyel Roh, Young Min Park, Il-Seok Park, Jung Je Park, Sung-Chan Shin, Soon-Hyun Ahn, Seongjun Won, Chang Hwan Ryu, Tae Mi Yoon, Giljoon Lee, Doh Young Lee, Myung-Chul Lee, Joon Kyoo Lee, Jin Choon Lee, Jae-Yol Lim, Jae Won Chang, Jeon Yeob Jang, Man Ki Chung, Yuh-Seok Jung, Jae-Gu Cho, Yoon Seok Choi, Jeong-Seok Choi, Guk Haeng Lee, and Phil-Sang Chung
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Mouth Neoplasms ,Squamous Cell Carcinoma ,Surgery ,Guideline ,Republic of Korea ,Medicine ,Otorhinolaryngology ,RF1-547 - Abstract
Korean Society of Thyroid-Head and Neck Surgery appointed a Task Force to provide guidance on the implementation of a surgical treatment of oral cancer. MEDLINE databases were searched for articles on subjects related to “surgical management of oral cancer” published in English. Results were restricted to systematic reviews, randomized control trials/controlled clinical trials, and observational studies. The quality of evidence was rated with use RoBANS (Risk of Bias Assessment Tool for Nonrandomized Studies) and AMSTAR (A Measurement Tool to Assess the Methodological Quality of Systematic Reviews). Evidence-based recommendations for practice were ranked according to the American College of Physicians grading system. Additional directives are provided as expert opinions and Delphi questionnaire when insufficient evidence existed. The Committee developed 68 evidence-based recommendations in 34 categories intended to assist clinicians and patients and counselors, and health policy-makers. Proper surgical treatment selection for oral cancer, which is directed by patient- and subsite-specific factors, remains the greatest predictor of successful treatment outcomes. These guidelines are intended for use in conjunction with the individual patient’s treatment goals.
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- 2019
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8. Initial Nutritional Status and Clinical Outcomes in Patients With Deep Neck Infection
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Marn Joon Park, Ji Won Kim, Yonghan Kim, Yoon Se Lee, Jong-Lyel Roh, Seung-Ho Choi, Sang Yoon Kim, and Soon Yuhl Nam
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Retrophnarynx Abscess ,Fasciitis ,Necrotizing ,Hypoalbuminemia ,Malnutrition ,Drainage ,Medicine ,Otorhinolaryngology ,RF1-547 - Abstract
Objectives The current study aims to determine the correlation between nutritional status upon presentation and disease severity, as well as treatment and survival outcomes. Methods Patients who were diagnosed with deep neck infection, underwent at least one surgical drainage/debridement, and had more than 1 week of hospitalization at a tertiary medical center from 2007 to 2015 were retrospectively included. Thereafter, initial serum albumin, C-reactive protein (CRP), and body mass index (BMI) were reviewed. Results A total of 135 patients were included in the final analysis. Accordingly, the proportion of patients with simultaneous mediastinitis (21.0%), necrotizing fasciitis (12.9%), disease extent >1 cervical level (72.6%), mean CRP (22.4 mg/dL), mean length of hospitalization (25.0 days), and mean 1-week follow-up CRP (7.2 mg/dL) was significantly higher in the hypoalbuminemia group (initial serum albumin 1 cervical level (2.12), initial serum CRP over 20 mg/dL (3.79), hospitalization of more than 14 days (4.10), 1-week follow-up CRP over 5 mg/dL (3.78), and increased duration for an over 50% decrease in initial CRP (2.70) (all P
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- 2018
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9. Epigenetic reprogramming of epithelial-mesenchymal transition promotes ferroptosis of head and neck cancer
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Jaewang Lee, Ji Hyeon You, Min-Su Kim, and Jong-Lyel Roh
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Ferroptosis ,Epithelial-mesenchymal transition ,E-cadherin ,ZEB1 ,Epigenetic reprogramming ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Ferroptosis is a newly defined form of cell death induced by iron-dependent accumulation of lethal lipid peroxidation. Ferroptosis represent a therapeutic strategy to suppress therapy-resistant cancer cells with more property of epithelial-mesenchymal transition (EMT). However, epigenetic reprogramming of EMT has been rarely studied in the context of ferroptosis susceptibility. Therefore, we examined the therapeutic potentiality of EMT epigenetic reprogramming in promoting ferroptosis in head and neck cancer (HNC) cells. The effects of ferroptosis inducers and EMT inhibition or induction were tested in HNC cell lines and mouse tumor xenograft models. These effects were analyzed concerning cell viability and death, lipid reactive oxygen species and iron production, labile iron pool, glutathione contents, NAD/NADH levels, and mRNA/protein expression. Cell density and the expression levels of E-cadherin, vimentin, and ZEB1 were associated with the different susceptibility to ferroptosis inducers. CDH1 silencing or ZEB1 overexpression increased the susceptibility to ferroptosis, whereas CDH overexpression or ZEB1 silencing decreased the susceptibility, in vitro and in vivo. Histone deacetylase SIRT1 gene silencing or pharmacological inhibition by EX-527 suppressed EMT and consequently decreased ferroptosis, whereas SIRT inducers, resveratrol and SRT1720, increased ferroptosis. MiR-200 family inhibitors induced EMT and increased ferroptosis susceptibility. In HNC cells with low expression of E-cadherin, the treatment of 5-azacitidine diminished the hypermethylation of CDH1, resulting in increased E-cadherin expression and decreased ferroptosis susceptibility. Our data suggest that epigenetic reprogramming of EMT contributes to promoting ferroptosis in HNC cells.
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- 2020
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10. Dihydrolipoamide dehydrogenase regulates cystine deprivation-induced ferroptosis in head and neck cancer
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Daiha Shin, Jaewang Lee, Ji Hyeon You, Doyeon Kim, and Jong-Lyel Roh
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Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Ferroptosis is a new form of regulated cell death driven by iron-dependent lipid peroxidation. Glutaminolysis and tricarboxylic acid cycle are involved in ferroptosis, but the underlying metabolic process remains unclear. We examined the role of dihydrolipoamide dehydrogenase (DLD) in ferroptosis induction in head and neck cancer (HNC). The effects of cystine deprivation or sulfasalazine treatment and of DLD gene silencing/overexpression were tested on HNC cell lines and mouse tumor xenograft models. These effects were analyzed with regard to cell death, lipid reactive oxygen species (ROS) and mitochondrial iron production, mitochondrial membrane potential, mRNA/protein expression, and α-ketoglutarate dehydrogenase (KGDH)/succinate/aconitase activities. Cystine deprivation induced ferroptosis via glutaminolysis. Cystine deprivation or import inhibition using sulfasalazine induced cancer cell death and increased lipid ROS and mitochondrial iron levels, which had been significantly decreased by short-interfering RNA (siRNA) or short hairpin RNA (shRNA) targeting DLD (P
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- 2020
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11. RITA plus 3-MA overcomes chemoresistance of head and neck cancer cells via dual inhibition of autophagy and antioxidant systems
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Daiha Shin, Eun Hye Kim, Jaewang Lee, and Jong-Lyel Roh
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Head and neck cancer ,RITA ,3-methyladenine ,Autophagy ,p62 ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Reactivation of p53 and induction of tumor cell apoptosis (RITA) is a small molecule that blocks p53–MDM2 interaction, thereby reactivating p53 in tumors. RITA can induce exclusive apoptosis in cancer cells independently of the p53 pathway; however, the resistance of cancer cells remains a major drawback. Here, we found a novel resistance mechanism of RITA treatment and an effective combined treatment to overcome RITA resistance in head and neck cancer (HNC) cells. The effects of RITA and 3-methyladenine (3-MA) were tested in different HNC cell lines, including cisplatin-resistant and acquired RITA-resistant HNC cells. The effects of each drug alone and in combination were assessed by measuring cell viability, apoptosis, cell cycle, glutathione, reactive oxygen species, protein expression, genetic inhibition of p62 and Nrf2, and a mouse xenograft model of cisplatin-resistant HNC. RITA induced apoptosis of HNC cells at different levels without significantly inhibiting normal cell viability. Following RITA treatment, RITA-resistant HNC cells exhibited a sustained expression of other autophagy-related proteins, overexpressed p62, and displayed activation of the Keap1-Nrf2 antioxidant pathway. The autophagy inhibitor 3-MA sensitized resistant HNC cells to RITA treatment via the dual inhibition of molecules related to the autophagy and antioxidant systems. Silencing of the p62 gene augmented the combined effects. The effective antitumor activity of RITA plus 3-MA was also confirmed in vivo in mouse xenograft models transplanted with resistant HNC cells, showing increased oxidative stress and DNA damage. The results indicate that RITA plus 3-MA can help overcome RITA resistance in HNC cells. Condensed abstract: This study revealed a novel RITA resistant mechanism associated with the sustained induction of autophagy, p62 overexpression, and Keap1-Nrf2 antioxidant system activation. The combined treatment of RITA with the autophagy inhibitor 3-methyladenine overcomes RITA resistance via dual inhibition of autophagy and antioxidant systems in vitro and in vivo.
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- 2017
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12. Accelerated oral wound healing using a pre-vascularized mucosal cell sheet
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Jaewang Lee, Eun Hye Kim, Daiha Shin, and Jong-Lyel Roh
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Medicine ,Science - Abstract
Abstract Cell sheets with pre-vascularization have recently been developed but remain relatively untested in oral wound healing. Therefore, we examined the potential utility of our newly developed pre-vascularized mucosal cell sheets in oral wound healing. Mucosal keratinocytes, fibroblasts, and endothelial progenitor cells were primarily cultured for in vitro cell expansion from mucosa and blood of Sprague-Dawley rats. Mucosal cell sheets were generated using cultured keratinocytes and plasma fibrin (K sheet) or keratinocytes and a mixture of fibrin, fibroblasts, and endothelial cells (PV sheet). Autologous sheets were transplanted on deep wounds in the buccal region of rats. The gross and histological characteristics of wound healing were compared among control wound, K sheet, and PV sheet groups. We successfully cultured and expanded keratinocytes, fibroblasts, and endothelial progenitor cells in vitro for generating mucosal cell sheets with or without pre-vascularization. In the in vivo oral wound model, compared with the control wound, the PV sheet group exhibited rapid wound closure more prominently than the K sheet group. The histological healing in the PV sheet group was similar to that in rat normal buccal mucosa without fibrosis. The pre-vascularized mucosal cell sheet exhibited in vivo efficacy in oral wound healing by promoting accelerated healing.
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- 2017
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13. Nrf2 inhibition reverses the resistance of cisplatin-resistant head and neck cancer cells to artesunate-induced ferroptosis
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Jong-Lyel Roh, Eun Hye Kim, Hyejin Jang, and Daiha Shin
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Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Artesunate, an anti-malarial drug, has been repurposed as an anticancer drug due to its induction of cell death via reactive oxygen species (ROS) production. However, the molecular mechanisms regulating cancer cell death and the resistance of cells to artesunate remain unclear. We investigated the molecular mechanisms behind the antitumor effects of artesunate and an approach to overcome artesunate resistance in head and neck cancer (HNC). The effects of artesunate and trigonelline were tested in different HNC cell lines, including three cisplatin-resistant HNC cell lines. The effects of these drugs as well as the inhibition of Keap1, Nrf2, and HO-1 were assessed by cell viability, cell death, glutathione (GSH) and ROS production, protein expression, and mouse tumor xenograft models. Artesunate selectively killed HNC cells but not normal cells. The artesunate sensitivity was relatively low in cisplatin-resistant HNC cells. Artesunate induced ferroptosis in HNC cells by decreasing cellular GSH levels and increasing lipid ROS levels. This effect was blocked by co-incubation with ferrostatin-1 and a trolox pretreatment. Artesunate activated the Nrf2–antioxidant response element (ARE) pathway in HNC cells, which contributed to ferroptosis resistance. The silencing of Keap1, a negative regulator of Nrf2, decreased artesunate sensitivity in HNC cells. Nrf2 genetic silencing or trigonelline reversed the ferroptosis resistance of Keap1-silenced and cisplatin-resistant HNC cells to artesunate in vitro and in vivo. Nrf2–ARE pathway activation contributes to the artesunate resistance of HNC cells, and inhibition of this pathway abolishes ferroptosis-resistant HNC. Condensed abstract: Our results show the effectiveness and molecular mechanism of artesunate treatment on head and neck cancer (HNC). Artesunate selectively killed HNC cells but not normal cells by inducing an iron-dependent, ROS-accumulated ferroptosis. However, this effect may be suboptimal in some cisplatin-resistant HNCs because of Nrf2–antioxidant response element (ARE) pathway activation. Inhibition of the Nrf2–ARE pathway increased artesunate sensitivity and reversed the ferroptosis resistance in resistant HNC cells. Keywords: Head and neck cancer, Artensunate, Ferroptosis, Nrf2, Reactive oxygen species, Resistance
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- 2017
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14. Guidelines for the Surgical Management of Laryngeal Cancer: Korean Society of Thyroid-Head and Neck Surgery
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Soon-Hyun Ahn, Hyun Jun Hong, Soon Young Kwon, Kee Hwan Kwon, Jong-Lyel Roh, Junsun Ryu, Jun Hee Park, Seung-Kuk Baek, Guk Haeng Lee, Sei Young Lee, Jin Choon Lee, Man Ki Chung, Young Hoon Joo, Yong Bae Ji, Jeong Hun Hah, Minsu Kwon, Young Min Park, Chang Myeon Song, Sung-Chan Shin, Chang Hwan Ryu, Doh Young Lee, Young Chan Lee, Jae Won Chang, Ha Min Jeong, Jae-Keun Cho, Wonjae Cha, Byung Joon Chun, Ik Joon Choi, Hyo Geun Choi, and Kang Dae Lee
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Laryngeal Neoplasms ,Surgery ,Practice Guideline ,Glottis ,Supraglottis ,Medicine ,Otorhinolaryngology ,RF1-547 - Abstract
Korean Society of Thyroid-Head and Neck Surgery appointed a Task Force to develop clinical practice guidelines for the surgical treatment of laryngeal cancer. This Task Force conducted a systematic search of the EMBASE, MEDLINE, Cochrane Library, and KoreaMed databases to identify relevant articles, using search terms selected according to the key questions. Evidence-based recommendations were then created on the basis of these articles. An external expert review and Delphi questionnaire were applied to reach consensus regarding the recommendations. The resulting guidelines focus on the surgical treatment of laryngeal cancer with the assumption that surgery is the selected treatment modality after a multidisciplinary discussion in any context. These guidelines do not, therefore, address non-surgical treatment such as radiation therapy or chemotherapy. The committee developed 62 evidence-based recommendations in 32 categories intended to assist clinicians during management of patients with laryngeal cancer and patients with laryngeal cancer, and counselors and health policy-makers.
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- 2017
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15. Retraction Note: Accelerated oral wound healing using a pre-vascularized mucosal cell sheet
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Jaewang Lee, Eun Hye Kim, Daiha Shin, and Jong-Lyel Roh
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Medicine ,Science - Abstract
Editor's Note: this Article has been retracted; the Retraction Note is available at https://doi.org/10.1038/s41598-021-82919-5 .
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- 2021
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16. Accuracy of Core Needle Biopsy Versus Fine Needle Aspiration Cytology for Diagnosing Salivary Gland Tumors
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In Hye Song, Joon Seon Song, Chang Ohk Sung, Jong-Lyel Roh, Seung-Ho Choi, Soon Yuhl Nam, Sang Yoon Kim, Jeong Hyun Lee, Jung Hwan Baek, and Kyung-Ja Cho
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Salivary gland neoplasms ,Biopsy, large-core needle ,Biopsy, fine-needle ,Parotid gland ,Submandibular gland ,Pathology ,RB1-214 - Abstract
Background: Core needle biopsy is a relatively new technique used to diagnose salivary gland lesions, and its role in comparison with fine needle aspiration cytology needs to be refined. Methods: We compared the results of 228 ultrasound-guided core needle biopsy and 371 fine needle aspiration procedures performed on major salivary gland tumors with their postoperative histological diagnoses. Results: Core needle biopsy resulted in significantly higher sensitivity and more accurate tumor subtyping, especially for malignant tumors, than fine needle aspiration. No patient developed major complications after core needle biopsy. Conclusions: We recommend ultrasoundguided core needle biopsy as the primary diagnostic tool for the preoperative evaluation of patients with salivary gland lesions, especially when malignancy is suspected.
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- 2015
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17. Risk Factors and Survival Outcomes for Patients With Anastomotic Leakage After Surgery for Head and Neck Squamous Cell Carcinoma
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Do-Youn Kim, Jong-Lyel Roh, Jong Woo Choi, Seung-Ho Choi, Soon Yuhl Nam, and Sang Yoon Kim
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Anastomotic leakage ,Head and neck squamous cell carcinoma ,Survival ,Risk factor ,Medicine ,Otorhinolaryngology ,RF1-547 - Abstract
ObjectivesThis study evaluated the risk factors for anastomotic leakage (AL) and survival outcomes in patients with head and neck squamous cell carcinoma (HNSCC).MethodsPatients with HNSCC who underwent surgery carrying potential AL from 2003 through 2009 were included in this study. Univariate and multivariate analyses were performed and patient survival was calculated by the Kaplan-Meier method.ResultsOf 232 eligible patients, 25 (10.8%) developed AL. Univariate analyses revealed that primary tumor site, salvage surgery, perineural invasion, radiotherapy, chemotherapy, and blood transfusion were significantly associated with the occurrence of AL (P0.1).ConclusionPatients who received salvage surgery and blood transfusion may require careful surveillance for development of AL, which has a tendency toward decreased survival.
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- 2014
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18. Chondroradionecrosis of the Larynx: Diagnostic and Therapeutic Measures for Saving the Organ from Radiotherapy Sequelae
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Jong-Lyel Roh
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Chondroradionecrosis ,Larynx ,Diagnosis ,Treatment ,Hyperbaric oxygen therapy ,Medicine ,Otorhinolaryngology ,RF1-547 - Abstract
ObjectivesChondroradionecrosis (CRN) of the larynx is a rare but fatal complication of radiotherapy. We determined the optimal diagnostic methodology and management of laryngeal CRN in six patients.MethodsWe retrospectively reviewed the records of six patients with Chandler grade IV laryngeal CRN who had received prior radiotherapy (mean total radiation dose, 66.7±4.5 Gy) at a tertiary care hospital. Two patients underwent transoral laser microresection of their laryngeal carcinoma plus postoperative radiotherapy. All patients underwent endoscopy, computed tomography (CT), positron emission tomography (PET), removal of necrotic tissue, biopsy under suspension laryngoscopy, administration of antibiotics, and hyperbaric oxygen therapy (HBO). Their diagnostic and therapeutic results were assessed.ResultsCT showed CRN of the anterior larynx in three patients and CRN of the posterior larynx in three patients, with one patient having a false-positive result on PET. HBO consisted of a mean of 36±6 dives. After early debridement and HBO, five patients showed CRN improvement, but one had aggravation and subsequently underwent total laryngectomy. None of these patients showed local tumor recurrence on pathologic examination or during a mean follow-up of 24 months.ConclusionAlthough CRN of the larynx may be detected by endoscopic and imaging work-ups, detection may require pathologic examination. Early debridement and HBO may effectively treat CRN, saving the functional larynx.
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- 2009
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19. Use of F-Fluorodeoxyglucose Positron Emission Tomography in Patients with Rare Head and Neck Cancers
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Jong-Lyel Roh, Byoung Jae Moon, Jae Seung Kim, Jeong Hyun Lee, Kyung-Ja Cho, Seung-Ho Choi, Soon Yuhl Nam, Bong-Jae Lee, and Sang Yoon Kim
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Head and neck neoplasms ,Fluorodeoxyglucose ,Positron-emission tomography ,Neoplasm staging ,Surveillance ,Medicine ,Otorhinolaryngology ,RF1-547 - Abstract
ObjectivesThe clinical utility of 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) has been demonstrated in major head and neck cancers (HNCs) but is unclear in rare HNCs. We therefore evaluated FDG PET in the management of patients with rare HNCs.MethodsFDG PET and CT/MRI scanning were performed at the initial staging and/or the follow-up in 24 patients with rare HNCs, 10 with melanoma, 9 with sarcoma, 3 with olfactory neuroblastomas, and 2 with basal cell carcinoma. The diagnostic accuracy of CT and FDG PET for detecting primary tumors and metastases were compared with a histopathologic reference. The association between the PET results and the clinicopathologic parameters predicting tumor invasion, histologic grade and disease-free survival (DFS), was assessed.ResultsThe overall accuracies of FDG PET and CT/MRI were 92% and 79%, respectively, for detecting primary tumors and 91% and 74%, respectively, for nodal metastases, but the differences were not significant due to the small number of patients. The sensitivity and specificity of FDG PET for detecting distant metastases and second primary tumors were 100% and 87%, respectively. Follow-up FDG PET correctly diagnosed locoregional recurrence in all 12 patients, as shown by biopsy, and distant metastases in 6 patients. However, thickness of melanoma, histologic grade of sarcoma, and DFS were not associated with tumor FDG uptake.ConclusionFDG PET may be useful for staging, posttreatment monitoring, and detection of distant metastases and second primary tumors in patients with rare HNCs.
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- 2008
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20. Is Routine Central Neck Dissection Necessary for the Treatment of Papillary Thyroid Microcarcinoma?
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Se Jun Choi, Tae Yong Kim, Jong Cheol Lee, Young Kee Shong, Kyoung-Ja Cho, Jin Sook Ryu, Jeong Hyun Lee, Jong-Lyel Roh, and Sang Yoon Kim
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Papillary microcarcinoma ,Central compartment ,Neck dissection ,Neoplasm metastasis ,Thyroglobulin ,Medicine ,Otorhinolaryngology ,RF1-547 - Abstract
ObjectivesIt remains unclear as to whether routine central neck dissection (CND) is necessary when performing surgery to treat patients with papillary thyroid microcarcinoma (PTMC). To determine the necessity for routine CND in PTMC patients, we reviewed the clinicopathologic and laboratory data of the patients of PTMC.MethodsBetween September 2001 and July 2005, 101 patients with PTMC and clinical N0 disease were retrospectively reviewed. The study cohort was devided into groups: the total thyroidectomy plus CND group (the CND group, N=48) and the total thyroidectomy without CND group (the no CND group, N=53). The serum stimulated thyroglobulin (Tg) levels were measured after surgery and prior to radioactive iodine ablation therapy (RAI) and at 6-12 months after RAI. Pathology, the Tg levels and recurrence data were compared between the 2 groups.ResultsCentral nodal metastases were found in 18 of the 48 CND patients (37.5%). The incidence of Tg levels >5 ng/mL at RAI was higher in the no CND patients and in the 18 node-positive CND patients compared with the 30 node-negative CND patients (22-24% vs. 3%, respectively, P=0.020-0.058). The difference when performing a similar comparison using a >2 ng/mL Tg threshold level showed no significance (10-11% vs. 4%, respectively, P>0.1). Two of the no CND patients and one node-positive CND patient had recurrences in the thyroid bed or lateral neck during a mean follow-up of 24 months.ConclusionThe data showed that occult metastasis to the central neck is common in PTMC patients. A CND provides pathologic information about the nodal metastases, and it potentially provides guidance for planning the postoperative RAI. However, the long-term benefit of CND on recurrence and survival remains somewhat questionable.
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- 2008
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21. Spontaneous Pharyngeal Perforation After Forceful Vomiting: The Difference from Classic Boerhaave's Syndrome
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Jong-Lyel Roh and Chan Il Park
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Spontaneous perforation ,Pharynx ,Boerhaave's syndrome ,Mechanism ,Management ,Medicine ,Otorhinolaryngology ,RF1-547 - Abstract
Boerhaave's syndrome is spontaneous transmural perforation of the esophagus, which occurs most often after forceful vomiting or retching. This commonly occurs in the lower third of the esophagus but spontaneous perforation of the pharynx or cervical esophagus is extremely rare. This case presented a 20-yr-old healthy man with spontaneous pharyngeal perforation after forceful vomiting who had no history of instrumentation, cervical trauma, or having eaten anything sharp. Cervical pain and crepitus were the early symptom and sign of pharyngeal perforation and the rupture was detected on gastrografin swallow and CT examinations. The rupture site was higher than the upper esophageal sphincter, differing from Boerhaave's syndrome. The patient was conservatively managed without significant morbidity and mortality. Although this may resolve without surgical intervention, the pharyngeal rupture should receive early detection and clinical attention for preventing potential morbidity by late diagnosis.
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- 2008
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22. Sialocele formation after partial parotidectomy: Dependent on remnant parotid exposure
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Jong-Lyel Roh
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Otorhinolaryngology - Published
- 2023
23. Promotion of ferroptosis in head and neck cancer with divalent metal transporter 1 inhibition or salinomycin
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Jaewang Lee and Jong-Lyel Roh
- Subjects
Cancer Research ,Cell Biology - Published
- 2023
24. Transoral excision of dermoid cyst: Is it a cosmetic approach that can ensure safety?
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Jong-Lyel Roh
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Surgery - Published
- 2023
25. Supplementary Figure S4 from A Novel Polyphenol Conjugate Sensitizes Cisplatin-Resistant Head and Neck Cancer Cells to Cisplatin via Nrf2 Inhibition
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Jong-Lyel Roh, Hyejin Jang, and Eun Hye Kim
- Abstract
Supplementary Figure S4. The cells were exposed to 5 µM DPP-23 for 72 h after 48-h scr siRNA and p53 and Nrf2 siRNA transfection. The apoptotic cell fractions were measured and the error bars represent the standard error of three replicates. * and **P < 0.01 relative to control and scr siRNA, respectively.
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- 2023
26. Supplementary Figure Legends from A Novel Polyphenol Conjugate Sensitizes Cisplatin-Resistant Head and Neck Cancer Cells to Cisplatin via Nrf2 Inhibition
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Jong-Lyel Roh, Hyejin Jang, and Eun Hye Kim
- Abstract
Supplementary Figure Legends
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- 2023
27. Data from A Novel Polyphenol Conjugate Sensitizes Cisplatin-Resistant Head and Neck Cancer Cells to Cisplatin via Nrf2 Inhibition
- Author
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Jong-Lyel Roh, Hyejin Jang, and Eun Hye Kim
- Abstract
Many cancer cells show acquired resistance to chemotherapeutic agents, such as cisplatin. This is a major cause of cancer treatment failure, and novel agents to overcome resistance are thus urgently required. A novel synthetic polyphenol conjugate, (E)-3-(3,5-dimethoxyphenyl)-1-(2-methoxyphenyl)prop-2-en-1-one (DPP-23), selectively kills tumor cells via the reactive oxygen species (ROS)–mediated unfolded protein response. We investigated the ability of DPP-23 to overcome cisplatin resistance in head and neck cancer (HNC) cells and further clarified its molecular mechanisms of action. Cisplatin-resistant HNC cell lines and their parental and other HNC cell lines were used. The effects of cisplatin and DPP-23 were assessed alone and in combination in HNC and normal cells using cell viability, cell cycle, and cell death assays, by measuring glutathione (GSH), ROS, and protein levels, and via preclinical mouse studies. DPP-23 induced selective cell death in HNC cells, including cisplatin-resistant HNC cells, but spared normal cells, via cellular GSH depletion and ROS accumulation. The effect was blocked by the antioxidant N-acetyl-L-cysteine. DPP-23 activated p53 and its related cell death pathways via a robust accumulation of cellular ROS that involved inhibition of nuclear factor erythroid 2–related factor 2 antioxidant defense mechanisms. Thus, DPP-23 significantly overcame cisplatin resistance in HNC cells in vitro and in vivo. As a promising anticancer strategy, ROS generation and subsequent selective cancer cell killing by DPP-23 might help to overcome cisplatin resistance in HNC. Mol Cancer Ther; 15(11); 2620–9. ©2016 AACR.
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- 2023
28. Supplementary methods from Inhibition of Glucosylceramide Synthase Sensitizes Head and Neck Cancer to Cisplatin
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Ji Won Kim, Jin Young Park, Eun Hye Kim, and Jong-Lyel Roh
- Abstract
Supplementary methods
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- 2023
29. Data from Inhibition of Glucosylceramide Synthase Sensitizes Head and Neck Cancer to Cisplatin
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Ji Won Kim, Jin Young Park, Eun Hye Kim, and Jong-Lyel Roh
- Abstract
Glucosylceramide synthase (GCS) overexpression is associated with multidrug resistance in several human cancers. GCS blockade, which overcomes multidrug resistance by downregulating P-glycoprotein (P-gp), has not been tested in head and neck cancer (HNC). This study investigates whether GCS is targetable in HNC by assessing whether GCS inhibition sensitizes HNC to cisplatin. The effect of genetic or pharmacologic GCS inhibition (using GCS siRNA/shRNA or d,l-threo-PPMP, respectively) on cisplatin sensitivity was assessed in several human HNC cells and acquired cisplatin-resistant HNC cells by measuring cell viability, cell cycle, death, mRNA and protein expression, ceramide production, and in preclinical tumor xenograft mouse models. GCS and P-gp expression were significantly associated with cisplatin resistance in several HNC cell lines (P = 0.007). Both were significantly increased in HN9-cisR cells, which display acquired cisplatin resistance (P < 0.001). Genetic or pharmacologic inhibition of GCS induced accumulation of increased ceramide levels. GCS inhibition increased cisplatin-induced cell death in HNC cells via P-gp downregulation and proapoptotic protein activation, which were abrogated by siPUMA transfection. Genetic and pharmacologic GCS inhibition sensitized resistant HNC cells to cisplatin in vitro and in vivo. GCS and P-gp overexpression is associated with acquired cisplatin resistance, suggesting a role for these molecules as therapeutic targets for HNC. Genetic or pharmacologic GCS blockade may have therapeutic benefit in cisplatin-resistant HNC. Mol Cancer Ther; 14(8); 1907–15. ©2015 AACR.
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- 2023
30. Altered iron metabolism as a target for ferroptosis induction in head and neck cancer
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Jaewang Lee and Jong-Lyel Roh
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Cancer Research ,Oncology ,Molecular Medicine ,General Medicine - Published
- 2023
31. Effect of the COVID-19 pandemic on surgery for indeterminate thyroid nodules (THYCOVID): a retrospective, international, multicentre, cross-sectional study
- Author
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Fabio Medas, Chiara Dobrinja, Ebtesam Abdullah Al-Suhaimi, Julia Altmeier, Said Anajar, Akif Enes Arikan, Irina Azaryan, Lovenish Bains, Giancarlo Basili, Hakan Bolukbasi, Marco Bononi, Farzad Borumandi, Mehmet Buğra Bozan, Gabriela Brenta, Laurent Brunaud, Maximilian Brunner, Antoine Buemi, Gian Luigi Canu, Federico Cappellacci, Sara Burchfield Cartwright, Ignasi Castells Fusté, Beatriz Cavalheiro, Giuseppe Cavallaro, Andres Chala, Shun Yan Bryant Chan, John Chaplin, Mustafa Sajjad Cheema, Costanza Chiapponi, Maria Grazia Chiofalo, Emmanuel Chrysos, Annamaria D'Amore, Michael de Cillia, Carmela De Crea, Nicolò de Manzini, Leandro Luongo de Matos, Loredana De Pasquale, Paolo Del Rio, Marco Stefano Demarchi, Muthuswamy Dhiwakar, Gianluca Donatini, Jose Miguel Dora, Valerio D'Orazi, Viyey Kishore Doulatram Gamgaram, Vitalijus Eismontas, El Hassane Kabiri, Hadj Omar El Malki, Islam Elzahaby, Octavian Enciu, Antoine Eskander, Francesco Feroci, David Figueroa-Bohorquez, Dimitrios Filis, Gorostidi François, Pedro Frías-Fernández, Armando Gamboa-Dominguez, Volkan Genc, Davide Giordano, Antonio Gómez-Pedraza, Giuseppa Graceffa, James Griffin, Sofia Cuco Guerreiro, Karan Gupta, Keshav Kumar Gupta, Angela Gurrado, Jiannis Hajiioannou, Tommi Hakala, Wirsma Arif Harahap, Lindsay Hargitai, Dana Hartl, Andrzej Hellmann, Jiri Hlozek, Van Trung Hoang, Maurizio Iacobone, Nadia Innaro, Orestis Ioannidis, J H Isabelle Jang, Jose Candido Xavier-Junior, Milan Jovanovic, Reto Martin Kaderli, Fahmi Kakamad, Krzysztof Kaliszewski, Martin Karamanliev, Hiroshi Katoh, Andro Košec, Bozidar Kovacevic, Luiz Paulo Kowalski, Robert Králik, Sanjay Kumar Yadav, Adriána Kumorová, Savvas Lampridis, Konstantinos Lasithiotakis, Jean-Christophe Leclere, Eugene Kwong Fei Leong, Melvin Khee-Shing Leow, James Y Lim, Leonardo S Lino-Silva, Shirley Yuk Wah Liu, Núria Perucho Llorach, Celestino Pio Lombardi, Javier López-Gómez, Eleonora Lori, Lourdes Quintanilla-Dieck, Roberta Lucchini, Amin Madani, Dimitrios Manatakis, Ivan Markovic, Gabriele Materazzi, Haggi Mazeh, Giuseppe Mercante, Goswin Yason Meyer-Rochow, Olgica Mihaljevic, Julie A Miller, Michele Minuto, Massimo Monacelli, Francesk Mulita, Barbara Mullineris, José Luis Muñoz-de-Nova, Fábio Muradás Girardi, Saki Nader, Tangjaturonrasme Napadon, Constantinos Nastos, Chiara Offi, Ohad Ronen, Luigi Oragano, Aida Orois, Yongqin Pan, Emmanouil Panagiotidis, Ramakanth Bhargav Panchangam, Theodosios Papavramidis, Pradipta Kumar Parida, Anna Paspala, Òscar Vidal Pérez, Sabrina Petrovic, Marco Raffaelli, Constanza Fernanda Ramacciotti, Tomas Ratia Gimenez, Ángel Rivo Vázquez, Jong-Lyel Roh, Leonardo Rossi, Alvaro Sanabria, Alena Santeerapharp, Arseny Semenov, Sanjeewa Seneviratne, Altinay Serdar, Patrick Sheahan, Sean C Sheppard, Rachel L Slotcavage, Constantin Smaxwil, Soo Young Kim, Salvatore Sorrenti, Eleftherios Spartalis, Chutintorn Sriphrapradang, Mario Testini, Yigit Turk, George Tzikos, Kristina Vabalayte, Kelly Vargas-Osorio, Rafael Sebastián Vázquez Rentería, David Velázquez-Fernández, Sanura Malinda Pallegoda Vithana, Levent Yücel, Erwin Danil Yulian, Petra Zahradnikova, Paul Zarogoulidis, Evgeniia Ziablitskaia, Anna Zolotoukho, Pietro Giorgio Calò, A Abdallah, AL Abentroth, V Acheimastos, M Agunaoun, HM Al Bisher, A Al Ghuzlan, H Alakus, M Alkan, MC Almaraz Almaraz, K Amram, S Anesidis, E Anestiadou, D Angelucci, GL Ansaldo, MI Antonopoulou, M Arciniegas, C Armellin, G Arredondo Saldaña, J Astl, E Athanasakis, S Avenia, H Aydın, B Baba, J Babala, MV Banús, LA Barba-Valadez, SV Barcons, F Battafarano, A Bayat, RMC Bella, F Benariba, S Bernardi, EG Bignami, M Bitenc, S Bitsianis, JDD Bolaños de la Torre, E Bonati, T Bonetti, FA Borges, K Bouchagier, M Boudina, A Bourial, I Breuskin, P Brock, C Bruns, MC Burlacu, T Burton, M Buta, D Buzanakov, C Caliseo, D Callanan, V Calu, JM Cameselle-Teijeiro, DJ Camilo-Junior, S Canberk, V Candalise, F Candanedo-Gonzalez, LJ Carrillo Lizarazo, GB Carvalho, D Casallas, C Casolino, L Castellani, C Castillo Morales, G Chambon, V Chatzipavlidou, R Chernikov, A Chorti, TCM Chow, A Chrisoulidou, E Chrysos, S Conrado-Neto, D Cordova García, A Corigliano, A Crocco, A Cuesta, M Čukman, LS Curto, RA Damilano, R D'Anna, M De, A De Virgilio, D Dellaportas, L Demarquet, A Devresse, G Di Meo, R Diaz Pedrero, D Dimitrov, Z Dmitry, P Domínguez Garijo, O Dulgeroglu, AC Dural, A Eksi, M El Hammoumi, H El Kaoui, G Eleni, A Elliyanti, Ş Ersöz, M Escobar-Jiménez, L Fedorova, L Feeley, E Fernández Rodríguez, F Ferreli, A Filoia, A Fingeret, A Francescato, F Gaino, F Galiandro, JF Gallegos-Hernández, G Garas, F García Lorenzo, JP García-Chávez, M Gaudiello, S Gay, S Gerasimos, M Gerek, R Gervasi, A Giordano, B Gjeloshi, L Gocký, E Golubinskaya, S González Romero, C González-Mínguez, M Goran, A Gosman, M Granados Garcia, E Greco, M Grünbart, R Grützmann, J Guerlain, XG Guirao, D Guzey, A Hajjij, O Hamdy, MS Hameed, LA Hauth, JD Hernández-Acevedo, JF Hernandez-Carrillo, F Hevilla Sánchez, H Hoi, K Hongkwon, R Hu Zhu, E Huang, K Hyeung Kyoo, V Ignjatovic, A Ioannidis, A Iossa, A Işık, D James, L Jung Hoon, H Kara, J Karajovic, D Kartini, D Khambri, I Kholová, M Kisiel, M Knežević, YQ Koh, C Konca, C Kosmidis, G Kotsovolis, LP Kowalski, R Kralik, P Kuczma, BG Kuravi, A Kurnia, V Kyriaki, CM Lai, B Lallemant, AA Lardhi, S Leboulleux, JW Lee, G Lelli, M Leutner, MY Lim, CM Lim, A Llanos, X Lo, T Loderer, MA López-Corrales, M Ludwig, FF Magnabosco, C Maheo, AL Maia, O Makay, P Maksimova, S Mallick, C Mallouk, Z Mamani, S Mandal, M Manyalich Blasi, G Marincola, M Marulanda, M Mavromati, S Mayilvaganan, S Metso, A Micalizzi, A Michalopoulos, K Min-Su, A Miron, AK Mishra, C Misso, C Mittermair, Y Morosán Allo, M Mourad, M Moysidis, F Nabhan, R Nasiri, C Nastos, KY Ngiam, C Nomine-Criqui, AM Ntziovara, JM Nuño Vázquez-Garza, V Nutautiene, K Obtulovičová, L O'Keeffe, NO Okudur, P Ossola, E Ovejero Merino, M Ozdemir, A Pangonis, SS Panigoro, A Panuzi, D Papaconstantinou, N Pardo Matamoros, S Paschou, A Pasculli, K Paterakis, K Peiris, F Pennestrì, M Peppa, P Perdikaris, I Perdikaris, RH Pérez-Soto, S Piana, M Piccoli, D Pietrasanta, G Placentino, I Pliakos, A Polistena, A Pongtippan, G Potard, V Quinn, P Rahul, T Ramos, A Rankin, P Ratnayake, J Reuto-Castillo, A Ridolfo, J Rios-Valencia, P Riss, E Rival, J Rivillas, D Roi, EM Rollo, A Romanchishen, M Romito, J Rotnagl, B Rovcanin, G Russo, M Sabol, S Saki, S Saleh, A Salih, A Saltiki, G Salvador-Camarmo, DK Samal, S Sánchez-Flores, K Sapalidis, D Sarin, H Sarin, N Savkovic, RS Scheffel, AL Scheinpflug, C Scheuba, N Scheyer, M Schmidt, O Senashova, E Serafini, ML Serrano Arévalo, J Shank, ML Shindo, M Shoshkova, M Shvan, M Sičák, TG Silva, O Simó Guerrero, V Skuletic, N Slijepcevic, Z Slovic, P Soares, A Somova, S Soto, S Spiezia, V Stankovic, KJ Stephenson, E Straub, M Summa, S Surani, AA Syed, S Symeonidis, A Taciak, M Tarallo, A Tarle, N Tasis, K Tausanovic, L Tchabashvili, M Thierry, U Tokarczyk, EA Toma, S Topuz, F Torresan, C Uras, C Vaccaro, Á Valdés de Anca, M Valentini, E Varaldo, JG Vartanian, GI Verras, A Vithanage, H Wijayalathge, P Wiriyaamornchai, YLC Wong, P Wongwattana, S Xenaki, S Xie, M Xu, W Yang, S Yilmaz, YF Yılmaz, T Yotsov, MT Zahid, A Zielke, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC/NEFR - Pôle de Néphrologie, and UCL - (SLuc) Service de néphrologie
- Subjects
Endocrinology ,Endocrinology, Diabetes and Metabolism ,Internal Medicine - Abstract
Since its outbreak in early 2020, the COVID-19 pandemic has diverted resources from non-urgent and elective procedures, leading to diagnosis and treatment delays, with an increased number of neoplasms at advanced stages worldwide. The aims of this study were to quantify the reduction in surgical activity for indeterminate thyroid nodules during the COVID-19 pandemic; and to evaluate whether delays in surgery led to an increased occurrence of aggressive tumours. In this retrospective, international, cross-sectional study, centres were invited to participate in June 22, 2022; each centre joining the study was asked to provide data from medical records on all surgical thyroidectomies consecutively performed from Jan 1, 2019, to Dec 31, 2021. Patients with indeterminate thyroid nodules were divided into three groups according to when they underwent surgery: from Jan 1, 2019, to Feb 29, 2020 (global prepandemic phase), from March 1, 2020, to May 31, 2021 (pandemic escalation phase), and from June 1 to Dec 31, 2021 (pandemic decrease phase). The main outcomes were, for each phase, the number of surgeries for indeterminate thyroid nodules, and in patients with a postoperative diagnosis of thyroid cancers, the occurrence of tumours larger than 10 mm, extrathyroidal extension, lymph node metastases, vascular invasion, distant metastases, and tumours at high risk of structural disease recurrence. Univariate analysis was used to compare the probability of aggressive thyroid features between the first and third study phases. The study was registered on ClinicalTrials.gov, NCT05178186. Data from 157 centres (n=49 countries) on 87 467 patients who underwent surgery for benign and malignant thyroid disease were collected, of whom 22 974 patients (18 052 [78·6%] female patients and 4922 [21·4%] male patients) received surgery for indeterminate thyroid nodules. We observed a significant reduction in surgery for indeterminate thyroid nodules during the pandemic escalation phase (median monthly surgeries per centre, 1·4 [IQR 0·6-3·4]) compared with the prepandemic phase (2·0 [0·9-3·7]; p
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- 2023
32. Functional gland-preserving surgery via periauricular incision for pleomorphic adenoma of the parotid gland
- Author
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Jong-Lyel Roh
- Subjects
Adult ,Male ,medicine.medical_specialty ,Adolescent ,Facial Paralysis ,Adenoma, Pleomorphic ,Pleomorphic adenoma ,Young Adult ,Postoperative Complications ,stomatognathic system ,medicine ,Paralysis ,Humans ,Parotid Gland ,Longitudinal Studies ,Radionuclide Imaging ,Aged ,Facial Nerve Injuries ,Salivary gland ,business.industry ,Recovery of Function ,General Medicine ,Middle Aged ,medicine.disease ,Facial nerve ,Parotid Neoplasms ,Parotid gland ,Surgery ,medicine.anatomical_structure ,Parotid Pleomorphic Adenoma ,Oncology ,Superficial Parotidectomy ,Patient Satisfaction ,Female ,Neoplasm Recurrence, Local ,Ultrasonography ,medicine.symptom ,Salivation ,business ,Organ Sparing Treatments - Abstract
Introduction Gland-preserving surgery is often used for benign tumours in the parotid gland. Partial superficial parotidectomy via a periauricular incision may bring satisfactory cosmetic outcomes but the disease control outcome remains unrevealed. This study evaluated functional and disease control outcomes after gland-preserving surgery via periauricular incision for pleomorphic adenoma of the parotid gland. Methods This longitudinal study included 248 consecutive patients with parotid pleomorphic adenoma who underwent the preservation of most normal parotid tissues and the facial nerve combined with the en-bloc resection of tumours via periauricular incision. Postoperative complications, subjective satisfaction, salivary function, and tumour recurrence were assessed in each patient. The secretory function of the salivary gland was measured using salivary scintigraphy at 6 months after surgery, and ultrasonography was regularly followed. Results Median tumour size was 2.5 cm (range, 0.8–5.2 cm) and median operation time was 55 min (range, 39–88 min). All tumours were safely removed by gland-preserving surgery via periauricular incision without extension to Blair or hairline incision and tumour spillage. Temporary and permanent paralysis of the facial nerve was 14 (5.6%) and none of the study patients, respectively. Postoperative complications were minor and Frey's syndrome was found in 6 (2.4%) patients. The Secretary function of the affected gland was equal to that of the unaffected gland. No patients had a recurrence for a median follow-up of 78 months (range, 24–126 months). Conclusions Functional gland-preserving surgery via periauricular incision can treat pleomorphic adenoma in the parotid gland with satisfactory functional, cosmetic, and disease control outcomes.
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- 2022
33. Early intervention of transoral laser-assisted marsupialization for adult epiglottic abscess
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Jong-Lyel Roh
- Subjects
Otorhinolaryngology - Published
- 2023
34. Transoral Complete vs Partial Excision of the Sublingual Gland for Plunging Ranula
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Jong-Lyel, Roh
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Sublingual Gland ,Otorhinolaryngology ,Oral Surgical Procedures ,Humans ,Salivary Ducts ,Surgery ,Ranula ,Mouth Floor - Abstract
Plunging ranula is a pseudocyst of saliva extravasated from the sublingual gland (SLG) to the submandibular space. This is treated by transoral excision of the SLG or transcervical cyst excision that might differently affect surgical morbidity and recurrence. This study compared the clinical outcomes of complete vs partial excision of the SLG for plunging ranula.A nonrandomized comparative study.Academic medical center.This study included 42 patients with plunging ranula who underwent complete or partial excision of the SLG with the evacuation of cystic content. Two surgical methods of complete or partial SLG resection were alternatively allocated to consecutive patients without randomization. The primary outcome was a postoperative recurrence. Secondary outcomes were operation time and complications.Complete and partial excision of the SLG was performed in 22 and 20 patients, respectively, without injury to the Wharton's duct or the lingual nerve. Postoperative complications in 42 patients were minor with temporary events: hematoma, 1 (5%); tongue numbness, 2 (5%); dysgeusia, 4 (9%); and dysphagia, 2 (5%), which did not differ between patients with complete and partial excision of the SLG (Complete SLG excision is preferred over partial SLG excision to treat plunging ranula for reducing postsurgical risks of complications and recurrence.
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- 2021
35. Selective deep lobe parotidectomy via retroauricular hairline (Roh's) incision for deep lobe parotid pleomorphic adenoma
- Author
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Jong-Lyel Roh
- Subjects
medicine.medical_specialty ,Surgical Wound ,Adenoma, Pleomorphic ,Sweating, Gustatory ,Deep lobe ,Pleomorphic adenoma ,Postoperative Complications ,Paralysis ,medicine ,Humans ,Parotid Gland ,General Dentistry ,Retrospective Studies ,business.industry ,Parotidectomy ,medicine.disease ,Facial nerve ,Lobe ,Parotid Neoplasms ,Parotid gland ,Surgery ,stomatognathic diseases ,medicine.anatomical_structure ,Parotid Pleomorphic Adenoma ,Otorhinolaryngology ,Neoplasm Recurrence, Local ,medicine.symptom ,business - Abstract
OBJECTIVE Deep lobe parotid tumour is commonly removed with the covering superficial lobe of parotid gland. Total or subtotal parotidectomy leads to an increase in surgical morbidity. This study evaluated recurrence and function after selective deep lobe parotidectomy via retroauricular hairline (Roh's) incision for pleomorphic adenoma. MATERIALS AND METHODS Twenty-eight patients with deep lobe parotid pleomorphic adenomas underwent selective deep lobe parotidectomy with preservation of the superficial lobe and the facial lobe via Roh's incision. Each patient was evaluated with any complications, cosmetic and salivary functions and local recurrence. RESULTS Superficial lobe-preserving surgery via Roh's incision was successfully applied to all patients without injury to the facial nerve and the Stensen's duct for a median operation time of 65 min. Facial nerve paralysis was found only temporarily in 9 (32%) patients, and other complications were minimal. None of the patients had postoperative Frey's syndrome. Salivary secretory function in the operated side was well preserved. No recurrence was found in the patients for a median follow-up of 94 months. CONCLUSIONS Selective deep lobe parotidectomy via Roh's incision is a reliable option of treatment for deep lobe parotid pleomorphic adenoma.
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- 2021
36. Functional gland-preserving surgery for submandibular gland pleomorphic adenoma
- Author
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Jong-Lyel Roh
- Subjects
Cancer Research ,medicine.medical_specialty ,Submandibular Gland ,Conventional surgery ,Adenoma, Pleomorphic ,Pleomorphic adenoma ,Postoperative Complications ,stomatognathic system ,Blood loss ,Salivary scintigraphy ,Paralysis ,Humans ,Medicine ,Longitudinal Studies ,Lingual nerve ,business.industry ,General Medicine ,medicine.disease ,Submandibular gland ,Surgery ,Parotid gland ,Submandibular Gland Neoplasms ,stomatognathic diseases ,medicine.anatomical_structure ,Oncology ,medicine.symptom ,business - Abstract
Conventional surgery for submandibular gland tumour is total excision of the gland affected by the tumour. Gland-preserving surgery is commonly used for benign tumours arising in the parotid gland but not in the submandibular gland. This study evaluated long-term oncological and functional outcomes after gland-preserving surgery in patients with pleomorphic adenoma of the submandibular gland via the submental approach. This longitudinal study included 105 consecutive patients with submandibular gland pleomorphic adenoma who underwent the gland-preserving surgery combined with the en-bloc resection of tumours via the submental approach. Salivary scintigraphy was performed 6 months after surgery, and ultrasonography was regularly followed. Intraoperative findings, postoperative complications, cosmetic and salivary functions, and tumour recurrence were assessed in these patients. Median tumour size and submental incision length were 2.0 and 3.3 cm, respectively. Median operation time and amount of blood loss were 25 min and 18.5 mL, respectively. None had marginal or lingual nerve paralysis and most patients were satisfied with incision scar and facial contour. The salivary function of the affected gland was equal to that of the unaffected gland. One patient (0.9%) had single nodular recurrence 54 months after surgery and others had no recurrence for follow-up of median 96 months. Pleomorphic adenoma of the submandibular gland can be safely removed by the gland-preserving surgery via the submental approach which has operation time, cosmetic and functional benefits with compromising oncological outcomes.
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- 2021
37. Epithelial-mesenchymal plasticity: Implications for ferroptosis vulnerability and cancer therapy
- Author
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Jaewang Lee and Jong-Lyel Roh
- Subjects
Oncology ,Hematology - Published
- 2023
38. Targeting GPX4 in human cancer: Implications of ferroptosis induction for tackling cancer resilience
- Author
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Jaewang Lee and Jong-Lyel Roh
- Subjects
Cancer Research ,Oncology - Published
- 2023
39. Induction of ferroptosis in head and neck cancer: A novel bridgehead for fighting cancer resilience
- Author
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Jaewang Lee and Jong-Lyel Roh
- Subjects
Cancer Research ,Oncology ,Head and Neck Neoplasms ,Iron ,Ferroptosis ,Humans ,Lipid Peroxidation ,Mitochondria - Abstract
Most head and neck cancers (HNCs) originate from mucosal epithelial cells and show epithelial traits. It often changes to a mesenchymal or poorly differentiated state as cancer progresses, leading to invasion, metastasis, and resistance to treatment. The loss of epithelial traits by the epithelial-mesenchymal transition may render resilient cancers vulnerable to a novel non-apoptotic regulated cell death ferroptosis by the iron-dependent accumulation of excessive lipid peroxidation. By regulating mitochondrial or iron metabolism, intracellular ferrous iron and lipid peroxidation accumulation can be boosted, making resistant cancer cells more susceptible to ferroptosis. This article discusses the potential effect of ferroptosis induction as a novel treatment for resilient HNCs.
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- 2022
40. The impact of skeletal muscle depletion on older adult patients with head and neck cancer undergoing primary surgery
- Author
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Soon Yuhl Nam, Seung-Ho Choi, Jong-Lyel Roh, Sang Yoon Kim, Jae Seung Kim, and Ah Ra Jung
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Sarcopenia ,medicine.medical_specialty ,Multivariate analysis ,Logistic regression ,Tertiary referral hospital ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Prospective Studies ,030212 general & internal medicine ,Muscle, Skeletal ,Prospective cohort study ,Aged ,Retrospective Studies ,Squamous Cell Carcinoma of Head and Neck ,business.industry ,Head and neck cancer ,Cancer ,Prognosis ,musculoskeletal system ,medicine.disease ,Head and neck squamous-cell carcinoma ,Oncology ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,Geriatrics and Gerontology ,business ,human activities - Abstract
Introduction Sarcopenia may result in negative outcomes in patients with cancer, but its impact on surgical and oncological outcomes in older adult patients with head and neck squamous cell carcinoma (HNSCC) has not been systematically studied. This study evaluated the clinical impact of sarcopenia on postsurgical and oncological outcomes in older adult patients with HNSCC. Methods This is a prospective study of 190 consecutive HNSCC patients aged ≥65 years who underwent curative surgery at a tertiary referral hospital. Sarcopenia was determined from measurement of the cross-sectional area of skeletal muscles at the level of the third lumbar vertebra on pretreatment images of whole body 18F-fluorodeoxyglucose positron emission tomography/computed tomography. Primary outcomes were early complications and overall survival. Factors of early complications and readmission were identified using binary logistic regression analyses, and factors of overall survival and disease-free survival were identified using univariate and multivariate Cox proportional hazards regression analyses. Results Pretreatment sarcopenia were found in 64 (33.7%) patients. In multivariate analysis, sarcopenia and N classification were significantly associated with early complications, while sarcopenia and T classification were associated with readmission. Independent factors of overall survival outcomes were age, sarcopenia, and extranodal extension (all P Conclusion Sarcopenia may predict early complications and survival after curative surgery in older adult patients with HNSCC.
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- 2021
41. A simple widening technique of postlaryngectomy tracheostomal stenosis: Anchoring to the medial clavicle
- Author
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Jong-Lyel Roh
- Subjects
Cancer Research ,Oncology ,Oral Surgery - Published
- 2023
42. Lipid metabolism alterations and ferroptosis in cancer: Paving the way for solving cancer resistance
- Author
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Jaewang Lee, Daiha Shin, and Jong-Lyel Roh
- Subjects
Pharmacology - Abstract
Cancer often perturbs lipid metabolism, which leads to the alteration of metabolism intermediates, contributing to their deregulated growth and metastasis. Alteration of lipid metabolism shifting to contain more polyunsaturated fatty acids (PUFAs) in membrane phospholipids (PLs) also leads to cancer therapy resistance. High amounts of PL-PUFAs render cancer cells more vulnerable to lipid peroxidation (LPO), predisposing them towards ferroptosis, a new form of iron-dependent oxidative regulated cell death. The commitment of cancer undergoing ferroptotic cell death depends on the adaptive lipidome remodeling, LPO patterns, and LPO scavenging ability in heterogeneous cancer cells. Ferroptosis is receiving attention in cancer research as treating cancers, altering membrane lipid homeostasis, and refractory from conventional therapies. Therefore, a better understanding of the molecular underpinning of lipid metabolism alterations may provide new opportunities for solving cancer resistance. This review intends to understand altered lipid metabolism in cancers and discuss lipid composition and metabolic processes associated with ferroptosis induction in cancers.
- Published
- 2023
43. Ferroptosis induction via targeting metabolic alterations in head and neck cancer
- Author
-
Jaewang Lee and Jong-Lyel Roh
- Subjects
Oncology ,Hematology - Abstract
Ferroptosis is a newly regulated cell death induced by the accumulation of iron-mediated lipid peroxidation. The alteration of cancer metabolism may contribute to proliferation, metastasis, and treatment resistance in human cancers, implicating the sensitivity to ferroptosis induction. Altered metabolism in cancer cells regulates oxidative stresses and changes metabolism intermediates, contributing to their deregulated growth and proliferation. Cancer metabolic changes toward the elevation of cellular free iron and polyunsaturated fatty acids sensitize cancer cells to lipid peroxidation toxicity tightly linked to ferroptosis. The altered metabolism in cancers can be served as a promising target to reverse cancer therapeutic resistance by ferroptosis induction to selectively kill cancer cells while sparing normal cells. The role of mitochondria and lipid metabolism in inducing ferroptosis in head and neck cancer (HNC) has been elucidated in previous studies. Ferroptosis is receiving attention in cancer research as treating cancers altering cellular metabolism and refractory from conventional therapies. More in-depth studies are needed to develop highly therapeutic drugs and practical methods to induce ferroptosis in diverse cancer cells and tumor microenvironments effectively. Therefore, this review intends to understand the altered metabolism and find new therapeutic possibilities using ferroptosis in HNC.
- Published
- 2023
44. Risk Factors for Feeding Tube Dependency in Patients Undergoing Function Preservation Surgery for Advanced-Stage Laryngohypopharyngeal Cancer
- Author
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Manal Bin-Manie, Yeonjoo Choi, Jong-Lyel Roh, Seung-Ho Choi, Soon Yuhl Nam, and Sang Yoon Kim
- Subjects
Speech and Hearing ,Otorhinolaryngology ,Gastroenterology - Abstract
Dysphagia and feeding tube dependency commonly occur in patients with laryngeal or hypopharyngeal cancer (LHC) during and after treatment, often leading to poor functional outcomes. Therefore, we examined the factors related to feeding tube dependency among advanced-stage LHC patients undergoing curative surgery. This study included 69 consecutive patients who underwent conservative surgery for previously untreated, advanced-stage LHC (squamous cell carcinoma) between 2006 and 2016. Persistent feeding tube dependency was defined as 1 year or more after treatment completion. Binary logistic regression analysis was used to determine the factors associated with reactive prolonged and persistent feeding tube dependency. Cox proportional hazard regression analysis was used to determine the association between feeding tube dependency and survival. None of the study patients had a prophylactic feeding tube, but 15 (21.7%) patients had reactive feeding tube placement for 3 months or more. A total of 9 (13.0%) patients had persistent feeding tube dependency. Univariate analysis showed that age, tracheostomy, and common terminology criteria for adverse events (CTCAE) ≥ 3 were significantly associated with reactive prolonged and persistent feeding tube dependency (all P 0.05). In the multivariate analysis, advanced age and CTCAE ≥ 3 remained the independent factors of reactive prolonged and persistent feeding tube dependency (all P 0.05). Feeding tube dependency was not associated with overall survival or disease-free survival (P 0.1). Feeding tube dependency might be related to clinical factors, such as age and severe adverse events, in the patients undergoing function-preserving surgery for advanced-stage LHC.
- Published
- 2022
45. Function-preserving surgery via single transverse cervical incision for Warthin's tumor in the parotid gland
- Author
-
Jong-Lyel Roh
- Subjects
Postoperative Complications ,Otorhinolaryngology ,Humans ,Parotid Gland ,Neoplasms, Second Primary ,Adenolymphoma ,Neck - Abstract
This study evaluated functional and disease control outcomes after gland-preserving surgery via a single transverse cervical incision for Warthin's tumor of the parotid gland.One-hundred eleven patients with Warthin's tumor underwent the preservation of most normal parotid tissues and the facial nerve combined with the en-bloc resection of tumors. Postoperative complications, subjective satisfaction, salivary function, and metachronous tumor were assessed in each patient.No patients had an extension to modified Blair or periauricular incision with the median operation time of 32 min. Temporary and permanent paralysis of the facial nerve was 5 (4.0%) and none of 125 tumor resection sides, respectively. Postoperative complications were minimal. The secretory functions between the affected and unaffected glands did not differ. One patient had metachronous tumor for a median follow-up of 72 months.Functional gland-preserving surgery via single transverse cervical incision can safely treat Warthin's tumor in the parotid gland.
- Published
- 2022
46. Removal of Thyroglossal Duct Cyst by a Submental Approach
- Author
-
Jong-Lyel Roh
- Subjects
Cicatrix ,Thyroid Gland ,Humans ,Pain ,Surgery ,Neck ,Thyroglossal Cyst - Abstract
Thyroglossal duct cyst (TGDC) is the most common congenital cyst in the neck and is removed by the Sistrunk procedure. This surgery involves a horizontal skin incision over the cyst that may leave a noticeable scar on the front of the neck. Therefore, this study examined the clinical outcomes and cosmetic benefits of the Sistrunk procedure by an incision in the submental area that is not easily visible from the front.This observational study was performed on 152 patients who underwent the Sistrunk procedure by a submental approach to remove TGDC at a university medical center. Intraoperative findings, postoperative complications, subjective pain levels, and satisfaction with incision scars and neck and facial deformities, and recurrence were prospectively evaluated.The length of the submental incision was about 3 cm and the median total operation time was 36 min. Postoperative complications were minimal. Hematoma occurred in two cases (1.3%), surgical site infection in 1 case (0.7%), and dysphagia for more than 1 week occurred in 1 case (0.7%). On a 0-10 visual analogue scale, the pain had a median value of 2 on the first day after surgery, and satisfaction with incision scars and neck and facial deformities showed median values of 8 and 10 at 6 months after surgery, respectively. Recurrence occurred in one patient (0.7%) during the median follow-up period of 68 months.The submental approach for TGDC excision may be a reliable new surgical method that is safe and has cosmetic advantages. This observational study evaluated the clinical outcomes and cosmetic benefits of the Sistrunk procedure by a submental incision for thyroglossal duct cyst in 152 patients. The surgical procedure showed no increased operation time, no need for wide flap elevation, easy suprahyoid dissection, and an invisible scar in a natural position of the neck.
- Published
- 2022
47. Prediction of cervical metastasis and survival in cN0 oral cavity cancer using tumour 18F-FDG PET/CT functional parameters
- Author
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Jae Seung Kim, Soon Yuhl Nam, Jong-Lyel Roh, Sang Yoon Kim, Seung-Ho Choi, and Mi Rye Bae
- Subjects
0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,medicine.diagnostic_test ,Lymphovascular invasion ,business.industry ,medicine.medical_treatment ,Cancer ,Neck dissection ,Standardized uptake value ,General Medicine ,medicine.disease ,Occult ,Metastasis ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,Positron emission tomography ,030220 oncology & carcinogenesis ,medicine ,Radiology ,Oral Cavity Squamous Cell Carcinoma ,business - Abstract
Oral cavity squamous cell carcinoma (OCC) can spread to the neck without apparent lymphadenopathy. Pretreatment detection or prediction of occult metastasis might contribute to proper management of clinically node-negative (cN0) OCC. We examined the role of tumour quantitative 18-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET)/computed tomography (CT) measurements for predicting OCC occult metastasis and survival. This study included 130 cN0 OCC patients who underwent 18F-FDG PET/CT scanning and subsequent curative surgery and neck dissection. Maximum, peak, and mean standardized uptake value (SUVmax, SUVpeak, and SUVmean), metabolic tumour volume (MTV), and total lesion glycolysis (TLG) were measured on pretreatment 18F-FDG PET/CT. Binary logistic regression was used to identify factors predicting occult cervical metastasis. Univariate and multivariate Cox proportional hazard regression were used to find factors associated with overall survival (OS). Pathological cervical metastasis (pN +) was found in 29 (22.3%) patients. Age, tumour differentiation, lymphovascular invasion, and T classification were significantly associated with pN + (all P 9.3 g had a 5.7-fold increased risk of overall mortality. Tumour 18F-FDG PET/CT parameters might predict occult metastasis and survival in cN0 OCC patients.
- Published
- 2020
48. Inhibition of Glutaredoxin 5 predisposes Cisplatin-resistant Head and Neck Cancer Cells to Ferroptosis
- Author
-
Ji Hyeon You, Daiha Shin, Jong-Lyel Roh, and Jaewang Lee
- Subjects
0301 basic medicine ,Male ,Iron ,Medicine (miscellaneous) ,Transferrin receptor ,Small hairpin RNA ,03 medical and health sciences ,gene silencing ,Mice ,0302 clinical medicine ,Cell Line, Tumor ,Antineoplastic Combined Chemotherapy Protocols ,Gene silencing ,Ferroptosis ,Animals ,Humans ,Viability assay ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Glutaredoxins ,biology ,Chemistry ,Xenograft Model Antitumor Assays ,glutaredoxin 5 ,Mitochondria ,Ferritin ,Sulfasalazine ,030104 developmental biology ,free iron ,Cell culture ,GLRX5 ,Drug Resistance, Neoplasm ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,Cancer cell ,Mutation ,biology.protein ,Cancer research ,cancer cells ,RNA Interference ,Cisplatin ,Research Paper - Abstract
Rationale: Loss of iron-sulfur cluster function predisposes cancer cells to ferroptosis by upregulating iron-starvation response, but the role of glutaredoxin 5 (GLRX5) silencing in ferroptosis remains unknown. We examined the role of GLRX5 functional loss in promoting ferroptosis in cisplatin-resistant head and neck cancer (HNC) cells. Methods: The effects of sulfasalazine treatment and GLRX5 gene silencing were tested on HNC cell lines and mouse tumor xenograft models. These effects were analyzed concerning cell viability and death, lipid reactive oxygen species (ROS) and mitochondrial iron production, labile iron pool, mRNA/protein expression, and malondialdehyde assays. Results: Cyst(e)ine deprivation, erastin, or sulfasalazine induced ferroptosis in HNC cells, which was relatively less sensitive in cisplatin-resistant HNC cells. Sulfasalazine or cyst(e)ine deprivation-induced ferroptosis resulted from increased lipid peroxidation and intracellular free iron, which were significantly promoted by short-interfering RNA or short hairpin RNA (shRNA) targeting GLRX5 (P
- Published
- 2020
49. Risk factors for survival and distant metastasis in 125 patients with head and neck adenoid cystic carcinoma undergoing primary surgery
- Author
-
Seung-Ho Choi, Jong-Lyel Roh, In Seong Jeong, Soon Yuhl Nam, Sang Yoon Kim, and Kyung-Ja Cho
- Subjects
Adult ,0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,Multivariate analysis ,Adolescent ,Adenoid cystic carcinoma ,Lymphovascular invasion ,medicine.medical_treatment ,Disease ,Metastasis ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,medicine ,Humans ,Neoplasm Metastasis ,Head and neck ,Aged ,Proportional Hazards Models ,Hematology ,business.industry ,General Medicine ,Middle Aged ,medicine.disease ,Carcinoma, Adenoid Cystic ,Surgery ,Survival Rate ,Radiation therapy ,030104 developmental biology ,Oncology ,Head and Neck Neoplasms ,Lymphatic Metastasis ,030220 oncology & carcinogenesis ,Radiotherapy, Adjuvant ,Neoplasm Recurrence, Local ,business - Abstract
Adenoid cystic carcinoma (AdCC) is generally slow growing but has highly metastatic potential to distant organs. Several factors and biomarkers are associated with metastasis and treatment outcomes, although further definition is needed. Therefore, this study aimed to evaluate the risk factors for survival and distant metastasis in patients with head and neck AdCC. This study included 125 patients with previously untreated AdCC who underwent primary surgery with or without radiotherapy in our tertiary referral centre. Univariate and multivariate Cox proportional hazard regression analyses were used to identify risk factors associated with overall survival (OS), cause-specific survival (CSS), disease-free survival (DFS), and distant metastasis-free survival (DMFS). Factors associated with OS in patients with distant metastasis were separately analysed. During a median follow-up of 9.8 years (range 3.0–22.6 years), 58 patients (46.4%) had distant metastasis and 29 (23.2%) died of disease. Multivariate analyses showed that lymphovascular invasion, lymph node metastasis, and distant metastasis were independent factors of OS and CSS outcomes (all P
- Published
- 2020
50. Positive lymph node number and extranodal extension for predicting recurrence and survival in patients with salivary gland cancer
- Author
-
Soon Yuhl Nam, Sang Yoon Kim, Kyung-Ja Cho, Jong-Lyel Roh, Hojun Lee, and Seung-Ho Choi
- Subjects
Oncology ,medicine.medical_specialty ,Multivariate analysis ,Lymph node metastasis ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Overall survival ,Humans ,Medicine ,Positive lymph node ,In patient ,030223 otorhinolaryngology ,Neoplasm Staging ,Retrospective Studies ,Extranodal Extension ,business.industry ,Cancer ,Prognosis ,Salivary Gland Neoplasms ,medicine.disease ,Survival Rate ,Otorhinolaryngology ,Salivary gland cancer ,Lymphatic Metastasis ,030220 oncology & carcinogenesis ,Lymph Nodes ,Neoplasm Recurrence, Local ,business - Abstract
BACKGROUND This study compared our proposed N classification including the addition of the number of positive lymph node (LN+) and extranodal extension (ENE) with the current and previous American Joint Committee on Cancer (AJCC) N classifications in salivary gland cancer (SGC). METHODS This study involved 172 SGC patients who underwent surgery. Cox proportional hazard regression analyses were used to identify factors associated with overall survival (OS) and disease-free survival (DFS). RESULTS In multivariate analyses, LN+ number, LN ratio, and ENE were associated with OS and DFS outcomes (all P
- Published
- 2020
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