1. FunResDB-A web resource for genotypic susceptibility testing of Aspergillus fumigatus
- Author
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Jörg Steinmann, Jonas Schaer, Birgit Spiess, Dieter Buchheidt, Kerstin Kaerger, Peter-Michael Rath, Michael Weber, Grit Walther, Oliver Kurzai, and Axel Hamprecht
- Subjects
0301 basic medicine ,Nonsynonymous substitution ,Azoles ,Antifungal Agents ,Genotype ,Genotyping Techniques ,Sequence analysis ,030106 microbiology ,Medizin ,Microbial Sensitivity Tests ,Aspergillosis ,medicine.disease_cause ,drug susceptibility ,Aspergillus fumigatus ,Fungal Proteins ,03 medical and health sciences ,Cytochrome P-450 Enzyme System ,Databases, Genetic ,medicine ,Humans ,Gene ,triazoles ,Alleles ,database ,Genetics ,Fungal protein ,Mutation ,Internet ,Polymorphism, Genetic ,biology ,Brief Report ,fungal infection ,General Medicine ,biology.organism_classification ,medicine.disease ,Editor's Choice ,Infectious Diseases - Abstract
Therapy of invasive aspergillosis is becoming more difficult due to the emergence of azole resistance in Aspergillus fumigatus. A majority of resistant strains carries mutations in the CYP51A gene. Due to a lack of sensitivity of culture-based methods, molecular detection of A. fumigatus has become an important diagnostic tool. We set up the database FunResDB (www.nrz-myk.de/funresdb) to gather all available information about CYP51A-dependent azole resistance from published literature. In summary, the screening resulted in 79 CYP51A variants, which are linked to 59 nonsynonymous mutations. A tailor-made online sequence analysis tool allows for genotypic susceptibility testing of A. fumigatus.
- Published
- 2016