1. Enzymatic solubilization of an insoluble substrate, fish protein concentrate: Process and kinetic considerations
- Author
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Daniel I. C. Wang, Jon O. Ragnarsson, Michael C. Archer, and Steven R. Tannenbaum
- Subjects
chemistry.chemical_classification ,Protease ,Chromatography ,biology ,Chemistry ,medicine.medical_treatment ,Kinetics ,Aqueous two-phase system ,Salt (chemistry) ,Substrate (chemistry) ,Bioengineering ,Bacillus subtilis ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Adsorption ,Enzyme ,medicine ,Biotechnology - Abstract
The solubilization of fish protein concentrate (FPC) by Bacillus subtilis protease (Monzyme) has been investigated. Conditions have been defined which eliminate the problems of microbial contamination and salt accumulation. A Kinetic treatment revealed that enzyme is adsorbed to the surface of the substrate, exposed to aqueous phase. The overall kinetics were described by a sequence of two first-order processes—an initial, fast reaction in which loosely bound polypeptide chains are cleaved from an insoluble protein particle, and a second, slower reaction in which more compacted core protein is digested.
- Published
- 1973
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