1. Comparison and evaluation of a high volume air sampling system for the collection of Clostridioides difficile endospore aerosol in health care environments
- Author
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Bradley S. Stevenson, Kathleen A.N. Aithinne, David L. Johnson, Casey W. Cooper, and Jon E. Black
- Subjects
Air sampling ,Epidemiology ,complex mixtures ,Endospore ,03 medical and health sciences ,0302 clinical medicine ,Clostridioides ,Medicine ,Humans ,030212 general & internal medicine ,Aerosolization ,Aerosols ,Spores, Bacterial ,0303 health sciences ,030306 microbiology ,business.industry ,Clostridioides difficile ,Health Policy ,fungi ,Public Health, Environmental and Occupational Health ,Environmental engineering ,Sampling (statistics) ,Contamination ,Aerosol ,Infectious Diseases ,Volume (thermodynamics) ,business ,Delivery of Health Care ,Environmental Monitoring - Abstract
Background Environmental contamination of patient rooms and adjacent areas with C. difficile spores is a recognized transmission risk. Previous studies have shown that spores are aerosolized during patient care. These spores can remain airborne for extended periods and may contaminate distant surfaces. High-volume air sampling equipment allows for the collection of a large volume of air and was evaluated in the collection of C. difficile aerosol. Method Air samplers evaluated in this research included the DFU-1000, XMX/2L-MIL, Biocapture-650, and a MB2. Aerosols of C. difficile were generated in a 5-m 3 chamber and each air sampler sampled in the aerosol test chamber simultaneously with referee air samplers. Results The DFU-1000 achieved the highest efficiency of the 4 air samplers (P = .0145) with a mean efficiency of 38.60%. The relative efficiencies of the Biocapture-650, XMX/2L-MIL, and MB2 were 28.16%, 10.51%, and 3.05%, respectively. Discussion/Conclusions This study demonstrated high variation based on the sampling method employed. Based on the results of these studies, high-volume air samplers may be effectively applied to sample for airborne C. difficile in health care environments. The high sampling flow rate of the DFU-1000 would allow for the complete sampling of a patient room-sized volume in less than 1 hour.
- Published
- 2019