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This article advocates for web scraping as an effective method to augment and enhance technical and professional communication (TPC) research practices. Web scraping is used to create consistently structured and well-sampled data sets about domains, communities, demographics, and topics of interest to TPC scholars. After providing an extended description of web scraping, the authors identify technical considerations of the method and provide practitioner narratives. They then describe an overview of project-oriented web scraping. Finally, they discuss implications for the concept as a sustainable approach to developing web scraping methods for TPC research.

Influenza virus infects millions of people annually and can cause global pandemics. Hemagglutinin (HA) is the primary component of commercial influenza vaccines (CIV), and antibody to HA is a primary correlate of protection. Persistent antigenic variation of HA requires that CIV be reformulated for new strains yearly. Differences in structural organization of HA has not been correlated with induction of broadly reactive antibodies, and CIV formulations can vary in how HA is organized. Using electron microscopy to study four current CIV, we found that these different formulations contained a variety of structures including: individual HAs, starfish-like structures with up to 12 HA molecules, and novel “spiked nanodisc” structures that displayed over 50 HA molecules along the complex’s perimeter. These spiked nanodiscs uniquely exposed conserved stem epitopes and elicited the highest levels of heterosubtypic cross-reactive antibodies. Overall, we found that HA structural organization can be an important CIV parameter and can be associated with the induction of cross-reactive antibodies to conserved HA epitopes.

Drug courts have been part of the criminal justice system for over 30 years. A plethora of quantitative quasi-experimental, experimental, and meta-analytic studies have demonstrated their effective...

This article uses a machine learning algorithm to demonstrate a proof-of-concept case for moderating and managing online comments as a form of content moderation, which is an emerging area of interest for technical and professional communication (TPC) researchers. The algorithm sorts comments by topical similarity to a reference comment/article rather than display comments by linear time and popularity. This approach has the practical benefit of enabling TPC researchers to reconceptualize content display systems in dynamic ways.

Drug courts are an alternative to incarceration for individuals who have substance use disorders and nonviolent arrests, and these programs can be an avenue to recovery for those who have opioid us...

Creating new emojis is predicated on a system of technical writing that lobbies for new emojis to the Unicode Consortium. Emojination, an activist collective working for cultural inclusivity, helps everyday people write proposals for inclusive and culturally sensitive emojis. Through a case study of Emojination, this article describes ways that Tactical Technical Communication can work toward cultural inclusivity within regulatory frameworks.

Chronos and kairos are often understood as separate from one another in discussions of rhetorical temporality. For online and other highly mediated contexts, however, chronos and kairos can be unde...

Drug courts are an alternative to incarceration for individuals who have substance use disorders. The drug court model is guided by key interventions (e.g., required treatment, frequent status hear...

Providing contextualized, effective writing instruction for engineering students is an important and challenging objective. This article presents a needs analysis conducted in a large engineering c...

Current influenza vaccines predominantly induce immunity to the hypervariable hemagglutinin (HA) head, requiring frequent vaccine reformulation. Conversely, the immunosubdominant yet conserved HA stem harbors a supersite that is targeted by broadly neutralizing antibodies (bnAbs), representing a prime target for universal vaccines. Here, we showed that the co-immunization of two HA stem immunogens derived from group 1 and 2 influenza A viruses elicits cross-group protective immunity and neutralizing antibody responses in mice, ferrets, and nonhuman primates (NHPs). Immunized mice were protected from multiple group 1 and 2 viruses, and all animal models showed broad serum-neutralizing activity. A bnAb isolated from an immunized NHP broadly neutralized and protected against diverse viruses, including H5N1 and H7N9. Genetic and structural analyses revealed strong homology between macaque and human bnAbs, illustrating common biophysical constraints for acquiring cross-group specificity. Vaccine elicitation of stem-directed cross-group-protective immunity represents a step toward the development of broadly protective influenza vaccines.

This article offers a methodology for conducting large-scale audience analysis called “big data audience analysis” (BDAA). BDAA uses distant reading and thin description to examine a large corpus o...

The present vaccine against influenza virus has the inevitable risk of antigenic discordance between the vaccine and the circulating strains, which diminishes vaccine efficacy. This necessitates new approaches that provide broader protection against influenza. Here we designed a vaccine using the hypervariable receptor-binding domain (RBD) of viral hemagglutinin displayed on a nanoparticle (np) able to elicit antibody responses that neutralize H1N1 influenza viruses spanning over 90 years. Co-display of RBDs from multiple strains across time, so that the adjacent RBDs are heterotypic, provides an avidity advantage to cross-reactive B cells. Immunization with the mosaic RBD–np elicited broader antibody responses than those induced by an admixture of nanoparticles encompassing the same set of RBDs as separate homotypic arrays. Furthermore, we identified a broadly neutralizing monoclonal antibody in a mouse immunized with mosaic RBD–np. The mosaic antigen array signifies a unique approach that subverts monotypic immunodominance and allows otherwise subdominant cross-reactive B cell responses to emerge. Antigenic variation of influenza A viruses necessitates the annual reformulation of vaccines. Kanekiyo et al. develop a mosaic nanoparticle vaccine against influenza virus that is able to elicit neutralizing antibodies that span nearly 100 years of variation of influenza A virus.

This article examines how empty state pages (ESPs) constrain user-generated communication through the ethical lens of Bourdieu’s habitus. The authors define ESPs as interactive instructiona...

Background Improving persuasion in response to vaccine skepticism is a long-standing problem. Elective nonvaccination emerging from skepticism about vaccine safety and efficacy jeopardizes herd immunity, exposing those who are most vulnerable to the risk of serious diseases. Objective This article analyzes vaccine sentiments in the New York Times as a way of improving understanding of why existing persuasive approaches may be ineffective and offers insight into how existing methods might be improved. We categorize pro-vaccine and anti-vaccine arguments, offering an in-depth analysis of pro-vaccine appeals and tactics in particular to enhance current understanding of arguments that support vaccines. Methods Qualitative thematic analyses were used to analyze themes in rhetorical appeals across 808 vaccine-specific comments. Pro-vaccine and anti-vaccine comments were categorized to provide a broad analysis of the overall context of vaccine comments across viewpoints, with in-depth rhetorical analysis of pro-vaccine comments to address current gaps in understanding of pro-vaccine arguments in particular. Results Appeals across 808 anti-vaccine and pro-vaccine comments were similar, though these appeals diverged in tactics and conclusions. Anti-vaccine arguments were more heterogeneous, deploying a wide range of arguments against vaccines. Additional analysis of pro-vaccine comments reveals that these comments use rhetorical strategies that could be counterproductive to producing persuasion. Pro-vaccine comments more frequently used tactics such as ad hominem arguments levied at those who refuse vaccines or used appeals to science to correct beliefs in vaccine skepticism, both of which can be ineffective when attempting to persuade a skeptical audience. Conclusions Further study of pro-vaccine argumentation appeals and tactics could illuminate how persuasiveness could be improved in online forums.

African swine fever virus (ASFV) is a complex nucleocytoplasmic large DNA virus (NCLDV) that causes a devastating swine disease currently present in many countries of Africa, Europe, and Asia. Despite intense research efforts, relevant gaps in the architecture of the infectious virus particle remain. Here, we used single-particle cryo-EM to analyze the three-dimensional structure of the mature ASFV particle. Our results show that the ASFV virion, with a radial diameter of ∼2,080 Å, encloses a genome-containing nucleoid surrounded by two distinct icosahedral protein capsids and two lipoprotein membranes. The outer capsid forms a hexagonal lattice (triangulation number T = 277) composed of 8,280 copies of the double jelly-roll major capsid protein (MCP) p72, arranged in trimers displaying a pseudo-hexameric morphology, and of 60 copies of a penton protein at the vertices. The inner protein layer, organized as a T = 19 capsid, confines the core shell, and it is composed of the mature products derived from the ASFV polyproteins pp220 and pp62. Also, an icosahedral membrane lies between the two protein layers, whereas a pleomorphic envelope wraps the outer capsid. This high-level organization confers to ASFV a unique architecture among the NCLDVs that likely reflects the complexity of its infection process and may help explain current challenges in controlling it.

Influenza virus continues to be a major health problem due to the continually changing immunodominant head regions of the major surface glycoprotein, hemagglutinin (HA). However, some emerging vaccine platforms designed by biotechnology efforts, such as recombinant influenza virus-like particles (VLPs) have been shown to elicit protective antibodies to antigenically different influenza viruses. Here, using biochemical analyses and cryo-electron microscopy methods coupled to image analysis, we report the composition and 3D structural organization of influenza VLPs of the 1918 pandemic influenza virus. HA molecules were uniformly distributed on the VLP surfaces and the conformation of HA was in a prefusion state. Moreover, HA could be bound by antibody targeting conserved epitopes in the stem region of HA. Taken together, our analysis suggests structural parameters that may be important for VLP biotechnology such as a multi-component organization with (i) an outer component consisting of prefusion HA spikes on the surfaces, (ii) a VLP membrane with HA distribution permitting stem epitope display, and (iii) internal structural components.

Prompted by an in-depth case study of a web-writer, this article argues that audience may be understood as an emergent process for web-writers who consider their comments. Rather than a group of people or demographic that is prefigured to exist, this article posits that audience might be a concept used throughout the composing process, including production and distribution processes. Such an approach is useful for digital pedagogies that involve online comments because it allows audience to be considered before, during, and after writers’ production processes.

Since 1989, drug courts have provided an alternative to incarceration for arrestees who have a substance use disorder. Previous research has suggested that participants who graduate from the program are less likely to recidivate than those who are terminated from the program. The majority of research on drug courts is quantitative; therefore, the benefits of qualitative methods are not fully seen in the literature. This qualitative study developed an in-depth understanding of participants’ (n = 42) views on the strengths and limitations of a Midwestern drug court. Two themes emerged from the data. First, participants felt that the drug court promoted camaraderie, which enhanced their motivation for change and supported them in graduating from the program. Second, participants felt that the drug court did not adequately treat their mental illnesses, which for some resulted in relapse and was perceived as a barrier to graduating from the program. Recommendations for drug court practice are discussed.

In this new book, Ruby Warrington utilizes research and personal experiences to propose a paradigm shift in how recovery from alcohol use disorders is conceptualized. She has unique experiences tha...

This article examines the role that algorithms may play as audiences when teaching writing on the World Wide Web. It argues that introducing the provisional term “algorithmic audience” reflects three prior conceptions of audience, including concrete situations, discourse community, and participatory audiences. It then offers a three-part classroom approach: identifying the biases of those who design algorithms, managing metadata, and anticipating audience response. I argue that the term “algorithmic audience” may help students to write for audiences beyond the instructor from within the confines of the classroom.

Motivational Interviewing was first introduced nearly three-and-a-half decades ago and has been used to treat a range of problems, including substance use disorders (SUDs). Treating SUDs is complex...

This research-based position paper offers critical examination and critique of the drug court (DC) graduation model and outlines an alternative approach. To enhance reconfiguration of the current DC graduation system, we propose a redemption-oriented framework that we believe is better aligned with the rehabilitation literature and reintegrative shaming theory. These conceptual underpinnings cohere with restorative justice and together represent a useful interpretive perspective for examining current DC practices in the United States. They also align with the goals we believe redemption rituals should elevate. This is based on four main elements of redemption rituals: achievement; co-ordination of care; status elevation; and moral inclusion. We operationalise these elements by identifying two constituent factors of each. The article concludes with a discussion of the implications for DC practice and recommendations for future research in this area.

Ribonucleoprotein (RNP) complexes of influenza viruses are composed of multiple copies of the viral nucleoprotein (NP) that can form filamentous supra-structures. RNPs package distinct viral genomic RNA segments of different lengths into pleomorphic influenza virions. RNPs also function in viral RNA transcription and replication. Different RNP segments have varying lengths, but all must be incorporated into virions during assembly and then released during viral entry for productive infection cycles. RNP structures serve varied functions in the viral replication cycle, therefore understanding their molecular organization and flexibility is essential to understanding these functions. Here, we show using electron tomography and image analyses that isolated RNP filaments are not rigid helical structures, but instead display variations in lengths, curvatures, and even tolerated kinks and local unwinding. Additionally, we observed NP rings within RNP preparations, which were commonly composed of 5, 6, or 7 NP molecules and were of similar widths to filaments, suggesting plasticity in NP-NP interactions mediate RNP structural polymorphism. To demonstrate that NP alone could generate rings of variable oligomeric state, we performed 2D single particle image analysis on recombinant NP and found that rings of 4 and 5 protomers dominated, but rings of all compositions up to 7 were directly observed with variable frequency. This structural flexibility may be needed as RNPs carry out the interactions and conformational changes required for RNP assembly and genome packaging as well as virus uncoating.

Drug courts have been a growing part of the criminal justice system since 1989. This qualitative study adds to the existing literature by developing an in-depth understanding of drug court from a woman’s (N = 25) perspective. Phenomenology and grounded theory guided the data collection and analysis, resulting in three themes. First, the women found the drug court team to be compassionate and empathetic, which they reported supported them in being successful in the program. Second, the majority of women reported histories of trauma and felt that the drug court could be improved by offering them more resources to treat their trauma. Third, the majority of women reported being single mothers and they shared examples of how being a single parent was a challenge to being successful in the drug court. Implications for drug court practice are discussed.

For nearly three decades, drug courts have provided a rehabilitative approach within the criminal justice system for individuals who have a substance use disorder. The goal of drug courts is to reduce criminal recidivism, and research has consistently suggested that participants that graduate drug court are less likely to recidivate than those who are terminated from the program. This qualitative study adds to the literature by asking drug court participants ( N = 42) their views on the most helpful aspects of the program that support them in graduating and how the program could be more helpful to support them in graduating. Two themes emerged from the data: (1) participants felt that interventions that are common to drug courts, such as drug testing and having frequent contact with the judge, were most helpful in supporting them in graduating the program; (2) participants felt that the agencies that offered treatment for their substance use disorders used punitive tactics and judgmental approaches that compromised the quality of treatment they received, and they felt that this was a barrier to them graduating the program. The findings are discussed in reference to drug court practice.

Unbroken Brain: A Revolutionary New Way of Understanding Addiction provides the reader with a critical analysis of addiction conveyed through the avenue of personal narrative, theoretical logic, an...

Negative-stain transmission electron microscopy (EM) is a technique that has provided nanometer resolution images of macromolecules for about 60 years. Developments in cryo-EM image processing have maximized the information gained from averaging large numbers of particles. These developments can now be applied back to negative-stain image analysis to ascertain domain level molecular structure (10 to 20 Å) more quickly and efficiently than possible by atomic resolution cryo-EM. Using uranyl acetate stained molecular complexes of influenza hemagglutinin bound to Fab 441D6, we describe a simple and efficient means to collect several hundred micrographs with SerialEM. Using RELION, we illustrate how tens of thousands of complexes can be auto-picked and classified to accurately describe the domain level topology of this unconventional hemagglutinin head-domain epitope. By comparing to the cryo-EM density map of the same complex, we show that questions about epitope mapping and conformational heterogeneity can readily be answered by this negative-stain method. © 2019 The Authors.

While digital writing and rhetoric scholars have critiqued interfaces from a design perspective and engaged speculative design, this chapter extends this work to the production of ethical interfaces through the framework of virtue ethics. The chapter offers a pedagogy of ethical interface production for instructors who teach interface design and social media rhetoric. Ethical interface production asks students to identify virtues, or character traits, they deem to be ethical and then produce multiple interfaces that seek to inculcate such habits.

Immunoelectron microscopy is a powerful technique for identifying viral antigens and determining their structural localization and organization within vaccines and viruses. While traditional negative staining transmission electron microscopy provides structural information, identity of components within a sample may be confounding. Immunoelectron microscopy allows for identification and visualization of antigens and their relative positions within a particulate sample. This allows for simple qualitative analysis of samples including whole virus, viral components, and viral-like particles. This article describes methods for immunogold labeling of viral antigens in a liquid suspension, with examples of immunogold-labeled influenza virus glycoproteins, and also discusses the important considerations for sample preparation and determination of morphologies. Together, these methods allow for understanding the antigenic makeup of viral particulate samples, which have important implications for molecular virology and vaccine development. © 2019 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.