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Objective To characterize the influence of mode (aerobic/resistance) and volume of exercise (moderate/high) on circulating GH immediately post-exercise as well as following the onset of sleep. Design This study used repeated measures in which subjects randomly completed 5 separate conditions: control (no exercise), moderate volume resistance exercise (MR), high-volume resistance exercise (HR), moderate volume aerobic exercise (MA), and high volume aerobic exercise (HA). Methods Subjects had two overnight stays on each of the 5 iterations. Serial blood draws began as soon as possible after the completion of the exercise session. Blood was obtained every 20 min for 24-h. GH was measured using a chemiluminescent immunoassay. Pooled samples representing post exercise (PE) and first nocturnal pulse (NP) were divided into two aliquots. One of these aliquots was chemically reduced by adding 10 mM glutathione (GSH) to break down disulfide-linked aggregates. Results No differences were observed when pooling GH response at post-exercise (2.02 ± 0.21) and nocturnal pulse (2.63 ± 0.51; p = .32). Pairwise comparisons revealed main effect differences between controls (1.19 ± 0.29) and both MA (2.86 ± 0.31; p = .009) and HA (3.73 ± 0.71; p = .001). Both MA (p = .049) and HA (p = .035) responses were significantly larger than the MR stimulus (1.96 ± 0.28). With GSH reduction, controls significantly differed from MA (p = .018) and HA (p = .003) during PE, but only differed from HA (p = .003) during NP. Conclusions This study demonstrated similar GH responses to exercise and nocturnal pulse, indicating that mode and intensity of exercise does not proportionately affect GH dimeric isoform concentration.

To determine if exercise training alters the pattern and magnitude of plasma concentrations of proenkephalin Peptide F and epinephrine, plasma proenkephalin [107–140] Peptide Fir and catecholamines were examined pre-training (T-1), and after 4- (T-2), 8- (T-3), and 12-weeks (T-4) of training. 26 healthy men were matched and randomly assigned to one of three groups: heavy resistance strength training (Strength, n = 9), high intensity endurance training (Endurance, n = 8), or both training modalities combined (Combined, n = 9). Blood was collected using a syringe with a cannula inserted into a superficial arm vein with samples collected at rest, after each 7 min stage and 5 and 15 min into recovery. With training, all groups observed shifted plasma Peptide F responses to graded exercise, where significant increases were observed at lower exercise intensities. Increases in plasma epinephrine with exercise were observed in all groups. The Combined group saw increases at 25% at T-3 and for 50% at T-2, T-3, and T-4 which was higher than T-1. The Endurance group demonstrated increases for 50% at T-1, T-2, T-3 but not at T-4. The plasma epinephrine response to graded exercise was reduced in the Strength group. Increases in plasma norepinephrine above rest were observed starting at 50% . The Strength group demonstrated a significant reduction in norepinephrine observed at 100% at T-3 and T-4. Peptide F and catecholamines responses to graded exercise can be altered by different types of physical exercise training. Simultaneous high intensity training may produce adrenal medulla exhaustion when compared to single mode training.

AB Introduction: The pulsatile secretion pattern of growth hormone (GH) is an important parameter of GH action at peripheral tissues, and more information is needed on how exercise impacts GH secretion. This study hypothesized that both aerobic and resistance exercise would exhibit dose-response relationships with respect to exercise duration and 20-h postexercise GH secretion. Methods: Eight healthy men randomly completed five separate conditions: 1) control (no exercise; CON), 2) a moderate-duration (1-h) aerobic exercise session (MA), 3) a long-duration (2-h) aerobic exercise session (LA), 4) a moderate-duration (1-h) resistance exercise session (MR), and 5) a long-duration (2-h) resistance exercise session (LR). Exercise intensity, diet, sleep, and physical activity were strictly controlled during each condition, and blood was sampled postexercise every 20 min for 20 h, and GH secretion parameters were analyzed via cluster and deconvolution analyses. Results: Only the 2-h aerobic exercise bout resulted in a significant amplification of GH secretion as evidenced by increases in GH burst peak amplitude (~100%), basal GH secretion rate (~127%), total GH basal secretion (~120%), total pulsatile secretion (~88%), and total GH secretion (~89%) over the control (i.e., no exercise) condition. GH secretions for the resistance exercise conditions were not different from control. Conclusions: The fact that the 2-h aerobic exercise condition resulted in higher energy expenditure than the other exercise conditions could offer a partial explanation for the greater GH amplification because of the metabolic effects that GH exerts in stimulating postexercise lipolysis. We conclude that extending the duration of aerobic exercise, but not resistance exercise, from 1- to 2-h significantly amplifies GH secretion during a 20-h period.

The GA-repeat binding protein (GABP) is a ubiquitous transcription factor involved in transcriptional regulation of genes encoding proteins involved in a variety of cellular processes including adipocyte differentiation, mitochondrial respiration, and neuromuscular signaling. GABP is composed of two subunits; the GABP alpha subunit is a member of the Ets-family of transcription factors, and the unrelated ankyrin repeat containing GABP beta subunit. We previously identified a bidirectional promoter directing the expression of Gabpa (GAA) gene in one direction and ATP Synthase Coupling Factor 6 (Atp5j) (CF6) gene in the other [Chinenov, Y., Coombs, C. and Martin, M. E., 2000a. "Isolation of a bi-directional promoter directing the expression of the mouse GABP alpha and ATP Synthase Coupling Factor 6 genes. Gene 261:311-320.]. In this study we characterize sequence elements and regulatory factors contributing to the promoter activities of the GAA/CF6 bidirectional promoter. The core of the GAA/CF6 bidirectional promoter is retained within a 400 bp sequence and contains four GABP binding sites, a Sp1/3 binding site and an YY1 binding site. Site-directed mutagenesis demonstrated that while no single factor binding site was essential for promoter activity in either direction, the GA1 site located proximal to the previously mapped transcription start sites functioned cooperatively with the other GABP binding sites and with the Sp1/3 and YY1 sites to provide transcriptional activation of the GAA and CF6 promoters. The other GABP sites and the Sp1/3 and YY1 binding sites were functionally redundant for basal promoter activities in both directions. Electrophoretic mobility shift assays identified multiple DNA-protein complexes containing GABP alpha, GABP beta, Sp1, Sp3 or YY1 proteins, including one ternary complex containing GABP alpha, GABP beta and Sp1 proteins. Binding of GABP to the GAA/CF6 bi-directional promoter provides the potential for autoregulation of GABP alpha expression and confirms the importance of GABP in the coordinate expression of respiratory chain components.

The pulsatile release of growth hormone (GH) and luteinizing hormone (LH) from the anterior pituitary gland is integral for signaling secretion of insulin-like growth factor (IGF)-I and testosterone, respectively. This study examined the hypothesis that 84 h of sustained physical exertion with caloric and sleep restriction alters the secretion of GH and LH. Ten male soldiers [22 yr (SD 3), 183 cm (SD 7), 87 kg (SD 8)] had blood drawn overnight from 1800 to 0600 every 20 min for GH, LH, and leptin and every 2 h for IGF-I (total and free), IGF binding proteins-1 and -3, testosterone (total and free), glucose, and free fatty acids during a control week and after 84 h of military operational stress. Time-series cluster and deconvolution analyses assessed the secretion parameters of GH and LH. Significant results (Por = 0.05) were as follows: body mass (-3%), fat-free mass (-2.3%), and fat mass (-7.3%) declined after military operational stress. GH and LH secretion burst amplitude (approximately 50%) and overnight pulsatile secretion (approximately 50%), IGF binding protein-1 (+67%), and free fatty acids (+33%) increased, whereas leptin (-47%), total (-27%) and free IGF-I (-32%), total (-24%) and free testosterone (-30%), and IGF binding protein-3 (-6%) decreased. GH and LH pulse number were unaffected. Because GH and LH positively regulate IGF-I and testosterone, these data imply that the physiological strain induced a certain degree of peripheral resistance. During periods of energy deficiency, amplitude modulation of GH and LH pulses may precede alterations in pulse numbers.

The purpose of this research was to determine the effects of high intensity endurance training (ET) and resistance training (RT) alone and in combination on various military tasks. Thirty-five male soldiers were randomly assigned to one of four training groups: total body resistance training plus endurance training (RT + ET), upper body resistance training plus endurance training [UB + ET), RT only, and ET only. Training was performed 4 days per week for 12 weeks. Testing occurred before and after the 12-week training regimen. All groups significantly improved push-up performance, whereas only the RT + ET group did not improve sit-up performance. The groups that included ET significantly decreased 2-mile run time, however, only RT + ET and UB + ET showed improved loaded 2-mile run time. Leg power increased for groups that included lower body strengthening exercises (RT and RT + ET). Army Physical Fitness Test performance, loaded running, and leg power responded positively to training, however, it appears there is a high degree of specificity when concurrent training regimens are implemented.

To characterize the impact of prolonged work, underfeeding, and sleep deprivation (i.e., sustained operations; SUSOPS) on physical and occupational related performance during military operational stress.Ten male soldiers were tested on days 1 (D1), 3 (D3), and 4 (D4) of a control and an experimental week that included prolonged physical work (total daily energy expenditure approximately 4,500 kcal x d(-1)), underfeeding (approximately 1,600 kcal x d(-1)), and sleep deprivation (approximately 2 h x d(-1)). Body composition was measured with dual-energy x-ray absorptiometry (DEXA). Ballistic power was assessed by 30 repetitive squat jumps and bench-press throws. Military-relevant occupational performance was evaluated with a 10-min box lift, obstacle course, grenade throw, rifle marksmanship, and a 25-min wall-build task.Fat-free mass (-2.3%) and fat mass (-7.3%) declined (P/= 0.05) during SUSOPS. Squat-jump mean power (-9%) and total work (-15%) declined (P/= 0.05) during SUSOPS. Bench-press power output, grenade throw, and marksmanship for pop-up targets were not affected. Obstacle course and box-lift performances were lower (P/= 0.05) on D3 but showed some recovery on D4. Wall building was approximately 25% lower (P/= 0.05) during SUSOPS.Decrements in performance during SUSOPS are primarily restricted to tasks that recruit muscles that are over-utilized without adequate recovery. General military skill tasks and occupational physical performance tasks are fairly well maintained.

Dietary creatine (Cr) supplementation has been shown to enhance muscular strength and endurance. This study determined the effects of Cr supplementation on performance of military training tasks. Two groups (Cr and placebo [Pl]) of 13 male soldiers each performed 3 consecutive military obstacle course runs ( approximately 3 minutes over 7 obstacles with a 2-minute rest between runs) followed by a rifle marksmanship task on 3 occasions (T(1), T(2), and T(3)), each separated by 5 days. They also completed a bench press protocol (5 sets to failure at 70% of 1 repetition maximum) and answered the Profile of Mood States questionnaire during each test session. Testing was done 3 times. No supplementation was given before T(1). Supplementation was provided using sports bars, with both groups receiving Pl bars between T(1) and T(2), whereas from T(2) to T(3) the Cr group consumed 24 g per day of Cr monohydrate in sports bars and the Pl group consumed an equal amount (kilocalories) of Pl sports bars. Creatine usage resulted in a significant (14%) increase in total bench press repetitions (p

To compare the physical fitness levels of recruits entering the U.S. Army in 1998 to those entering in 1978 and 1983.In 1998, 182 men and 168 women were tested before beginning basic training at Fort Jackson, SC. The measurements were 1) skin-fold estimation of percent body fat (%BF); 2) maximum oxygen uptake by treadmill running (VO2max); and 3) upper-body (UB), lower-body (LB), and upright pulling (UP) isometric strength. These data were compared to data from basic trainees at Fort Jackson in 1978 (skin folds, VO2max, UB, and LB) and 1983 (skin folds and UP).Body weight (BW) of 1998 recruits was greater (P0.05) than 1978 recruits (men, 12%; women, 6%) and 1983 recruits (men, 8%; women, 7%). %BF of 1998 recruits was greater (P0.05) than 1978 recruits (men, 15%; women, 5%) and 1983 recruits (men, 15%; women, 17%). The 1998 men had more fat-free mass (FFM) (P0.05) than men in 1978 (8%) or 1983 (5%), whereas 1998 women were only different from those measured in 1978 (4%, P0.05). The VO2max of men (50.6 +/- 6.2 mL x kg(-1) x min(-1)) was equivalent to men in 1978, whereas that of women (39.7 +/- 5.2 mL x kg(-1) x min(-1)) was 6% greater (P0.05). The 1998 recruits were stronger (P0.05) on all measures of muscle strength than recruits measured in 1978 (men, UB = 16%, LB = 12%; women, UB = 18%, LB = 6%) and 1983 (men, UP = 7%; women, UP = 6%).The aerobic capacity, muscle strength, and FFM of 1998 recruits is comparable to or greater than that of 1978 and 1983 recruits; however, 1998 recruits tended to have more BW and a greater %BF.

The purpose of this study was to determine the extent to which different types of audio display technology affected the ability of the physically active, load-carrying dismounted soldier to understand and respond to multiple radio communications in the battlefield. Independent variables were different types of auditory display configuration (existing monaural and spatial audio), number of simultaneous talkers in each simulated radio message (two, three, or four), and soldier rucksack load (22 kg or 33 kg). The dependent variables included the response time and number of accurate responses to the radio messages, soldier ratings of mental workload, and soldier physiological workload. Subjects were nine (9) male Marine Corps Infantry personnel and three (3) male Army Infantry personnel. Results indicated that spatial auditory displays enabled soldiers to identify a significantly greater number of simulated radio communications, and respond to these communications more quickly. Message response time increased and identification accuracy decreased as the number of simultaneous talkers increased. Rucksack weight was a predominant variable in physical and mental workload. Soldiers showed significantly greater physiological energy expenditure and significantly greater mental workload when they carried the heavier rucksack. The results indicated that whatever the load carried by the soldier, the speed and accuracy of understanding and responding to multiple radio communications were enhanced by presentation in different spatial locations.

It has been reported that plasma choline levels decrease following certain types of strenuous exercise. Preliminary findings also suggest that a drop in plasma choline may limit physical performance, while choline supplementation may delay fatigue during prolonged efforts. A double-blind crossover design was used to determine the relationship between plasma choline and performance during and after 4 hr of strenuous exercise. Volunteers (N = 14) received either a placebo or treatment beverage (8.425 g choline citrate) prior to and midway through a 4-hr load carriage treadmill exercise (3% grade at 5.6 km/h × 20 km) carrying a total load of 34.1 kg. Following the treadmill test, run time-to-exhaustion and squat tests were performed, and perceived exertion, plasma choline, glycerophosphocholine, and phosphatidylcholine were measured. Plasma choline levels increased 128% after the run-to-exhaustion with the choline supplemented beverage but remained unchanged with the placebo beverage. No significant effects were seen with choline supplementation on any outcome performance measure. Consequently, soldiers conditioned to carry heavy loads over long distances do not deplete plasma choline as a result of a prolonged exhaustive exercise under a placebo beverage, nor do they benefit from choline supplementation to delay fatigue under the same conditions.

The purpose of this study was to investigate the acute responses of both stress and fluid regulatory hormones to a single bout of resistance exercise in both trained and untrained men. Seven competitive power lifters (PL) and 12 untrained subjects (UT) performed one set of the leg press exercise to exhaustion at 80% of their respective one-repetition maximum. Blood samples were obtained twice prior to exercise (at P1 and P2), immediately postexercise (IP), and at 5 minutes postexercise (5PE). Compared to P1 and P2, plasma epinephrine, norepinephrine, dopamine, atrial peptide, osmolality, and blood lactic acid increased significantly (p

The primary purpose of this study was to examine the relationship between the lifting strength of soldiers entering the Army and the physical demands of their job. The muscle strength and body composition of 169 male and 153 female recruits were measured prior to basic training. Each soldier's lifting capacity was compared to the lifting standards of their assigned job. Nearly all soldiers were able to meet the standard for frequent lifting; however, 56% and 77% of women could not meet the standard for occasional lifting in the heavy (45.4 kg) and very heavy (>45.4 kg) physical demand category, respectively.

An avirulent, novel variant of equine arteritis virus (EAV; CA95G) was isolated from the semen of a persistently infected Standardbred stallion. The CA95G virus caused subclinical infection and seroconversion in susceptible horses, and virus was isolated only once from blood and nasal secretions collected from 6 experimentally infected horses. Sequence analysis of genes encoding the known EAV structural proteins shows that this highly attenuated strain of EAV is genetically similar to virulent field strains of EAV and, in particular, to a strain of EAV that was isolated during an outbreak of equine viral arteritis in western Canada in 1986. Not only is the carrier stallion the critical natural reservoir of EAV, but genetic diversity of the virus is generated in the course of persistent infection of carrier stallions. The subtle genetic changes that facilitate and maintain persistent EAV infection of the stallion's reproductive tract likely influence phenotypic properties of the virus such as virulence.

Previous studies have demonstrated that full recovery from weight loss may take months or years. The present investigation examined short-term recovery (5 wks "post") of physical performance (muscular strength, muscular power, vertical jump), body composition, metabolic hormones (testosterone, luteinizing hormone, sex hormone binding globulin, insulin-like growth factor-1, triiodothyronine, thyroxine, thyroid binding globulin, and thyroid-stimulating hormone) and metabolic markers (transferrin, ferritin, prealbumin, glycerol, nonesterified fatty acids, high-density lipoproteins, and lactate) in 10 healthy young men after an 8-week Army course with an energy deficit (1000 kcal/d) and loss of body mass (-12%). Subjects ate ad libitum after the course ended ("post"). Body composition was determined by dual-energy X-ray absorptiometry; strength from a simulated power clean, power from body mass and jump height, and metabolic hormones were measured in morning-fasted blood by radioimmunoassay. With the exception of transferrin and glycerol, all study parameters were significantly (p

The N-terminal hydrophilic ectodomain of the G(L) envelope glycoprotein of equine arteritis virus (EAV) contains neutralization determinants of the virus. We developed a panel of 17 neutralizing murine monoclonal antibodies (MAbs) to further characterize the neutralization determinants of EAV. Included were 6 MAbs previously raised against a laboratory strain (EAVUCD) of the original Bucyrus strain of EAV, as well as 11 additional MAbs that were raised against a neutralization-resistant variant [escape mutant (EM)] virus (EM6D10) that was derived from EAVUCD. All MAbs raised against EAVUCD and 4 of the MAbs raised against EM6D10 (2B3, 5F8, 8D4, and 10B4) reacted with the corresponding G(L) envelope glycoprotein in a Western immunoblotting assay, whereas the remaining 7 MAbs raised against EM6D10 did not react with any viral protein in the immunoblotting assay but competitively inhibited the binding of MAbs 2B3, 5F8, 8D4, and 10B4, indicating that they also recognize epitopes on the G(L) protein. A panel of 18 EM viruses raised to the MAb panel, 19 field isolates of EAV from North America and Europe, the modified-live virus vaccine (ARVAC), and 3 other laboratory strains of EAV were characterized by microneutralization assay with the panel of neutralizing MAbs and polyclonal rabbit and horse antisera. Comparative analysis of the nucleotide sequences of ORF5 and the deduced amino acid sequences of the G(L) protein of individual EM viruses and field isolates of EAV identified four distinct neutralization sites. These sites include amino acids 49 (site A), 61 (site B), 67 through 90 (site C), and 99 through 106 (site D). With the notable exception of site A, the sites were all located in the V1 variable region (amino acids 61-121) within the second half of the N-terminal hydrophilic ectodomain of the G(L) protein. Site D includes several overlapping linear epitopes which appear to interact with amino acids in the other three sites to form conformationally dependent epitopes. Amino acid substitutions within any of these four sites can alter the neutralization phenotype of individual strains of EAV.

The Department of Defense spends approximately one million dollars annually on research to enhance soldier physical performance (LTC K.E. Friedl, personal communication, Jan 1996). To most effectively direct this research effort, an accurate understanding of the physical demands of Army jobs is needed. The physical demands are available in printed form, however, there is no computerized means to quickly access and compile this information. The purpose of this paper is to describe the creation of a series of data bases containing the physically demanding tasks of Army occupations and to provide a preliminary summary of a selected data base.

Thirty-five healthy men were matched and randomly assigned to one of four training groups that performed high-intensity strength and endurance training (C; n = 9), upper body only high-intensity strength and endurance training (UC; n = 9), high-intensity endurance training (E; n = 8), or high-intensity strength training (ST; n = 9). The C and ST groups significantly increased one-repetition maximum strength for all exercises (P < 0.05). Only the C, UC, and E groups demonstrated significant increases in treadmill maximal oxygen consumption. The ST group showed significant increases in power output. Hormonal responses to treadmill exercise demonstrated a differential response to the different training programs, indicating that the underlying physiological milieu differed with the training program. Significant changes in muscle fiber areas were as follows: types I, IIa, and IIc increased in the ST group; types I and IIc decreased in the E group; type IIa increased in the C group; and there were no changes in the UC group. Significant shifts in percentage from type IIb to type IIa were observed in all training groups, with the greatest shift in the groups in which resistance trained the thigh musculature. This investigation indicates that the combination of strength and endurance training results in an attenuation of the performance improvements and physiological adaptations typical of single-mode training.

This study hypothesized that insulin-like growth factor-binding protein (IGFBP), rather than insulin-like growth factor I (IGF-I) itself, would be more responsive to acute exercise stress in a dose-dependent fashion.Eight men (24 +/- 5 yr, 87 +/- 9 kg, 182 +/- 6 cm, 21 +/- 5% body fat) had blood drawn every 4 h after exercise for 24 h and assayed for IGF-I, IGFBP-1, -3, -6, the acid labile subunit (ALS), insulin, glucose, and nonesterified free fatty acids on five occasions: no exercise (control, C), moderate-duration resistance exercise (MDRE; 25, 5-10 repetition maximum (RM) sets), long-duration resistance exercise (LDRE; 50, 5-10 RM sets), moderate-duration aerobic exercise (MDAE; three 15-min cycling bouts at approximately 70% (.)VO2peak), and long-duration aerobic exercise (LDAE; six 15-min cycling bouts at approximately 70% (.)VO2peak). Energy requirements were determined from resting metabolic rate, age, and a physical activity factor. Dietary control was implemented by providing all meals during the experimental trials. A two-way ANOVA with repeated measures (P0.05) was used for statistical analysis.Significant exercise effects were observed for IGFBP-1 (C: 14.0 +/- 2.7MDRE: 35.9 +/- 8.6 = LDRE: 45.2 +/- 10.6 = MDAE: 34.2 +/- 7.4 = LDAE: 47.0 +/- 11.8 ng x mL(-1) and insulin (C: 26.0 +/- 9LDRE: 13.2 +/- 6 ng x mL). In addition, a dose-response relationship was observed for the IGFBP-1 response (long-duration exercise (46 +/- 10 ng x mL(-1))moderate-duration exercise (35 +/- 7 ng x mL(-1)). There were no exercise effects for total IGF-I, IGFBP-3, and ALS. Effects of time of day were observed for all variables except ALS.For the circulating IGF-I system components measured, only IGFBP-1 seems to be a sensitive biomarker capable of assessing the physiological strain of acute physical exercise.

A poxvirus was isolated during the latter half of 1993 from a black-tailed deer (Odocoileus hemionus columbianus) that died of fulminant adenovirus infection in California (USA). The poxvirus was isolated from a pooled tissue homogenate, after repeated serial blind passages in primary black-tailed deer testicular cells. Based on electron microscopic examination of the virus, we observed morphologic features typical of the genus Orthopoxvirus, although definitive characterization was not done.

Insulin-like growth factor (IGF) I and IGF binding proteins (IGFBPs) modulate metabolic activity and tissue repair and are influenced by nutritional status. IGF-I circulates in free, ternary [IGF-I + IGFBP-3 + acid labile subunit (ALS)], and binary (IGF-I + IGFBP) molecular complexes, and the relative proportions regulate IGF-I extravascular shifting and bioavailability. This study examined the hypothesis that sustained physical activity and sleep deprivation superimposed on a short-term energy deficit would alter the IGFBP concentrations and alter the proportions of IGF-I circulating in ternary vs. binary molecular complexes. Components of the IGF-I system (total and free IGF-I; IGFBP-1, -3, and ALS; nonternary IGF-I and IGFBP-3), biomarkers of metabolic and nutritional status (transferrin, ferritin, prealbumin, glucose, free fatty acids, glycerol, beta-hydroxybutyrate), and body composition were measured in 12 men (22 +/- 3 yr, 87 +/- 8 kg, 183 +/- 7 cm, 20 +/- 5% body fat) on days 1, 3, and 4 during a control and experimental (Exp) period. During Exp, subjects performed prolonged work (energy expenditure of approximately 4500 kcal/day) with caloric (1600 kcal/day) and sleep (6.2 h total) restriction. IGF-I and IGFBP-3 were measured by immunoassay before and after immunoaffinity depletion of ALS-based complexes (i.e., ternary complex removal). Exp produced losses in body mass (-3.0%), lowered total IGF-I (-24%), free IGF-I (-42%), IGFBP-3 (-6%), nonternary IGF-I (-27%), and IGFBP-3 (-16%), and increased IGFBP-1 (256%). No Exp effects were observed for ALS. No changes were observed in the proportion of IGF-I circulating in free ( approximately 1.2%), ternary ( approximately 87.4%), or nonternary ( approximately 11.4%) molecular complexes. During Exp, glucose concentrations were lower on day 3, but days 1 and 4 were statistically similar. In conclusion, during a short-term energy deficit in young, healthy men, 1). IGF-I system components differentially respond (both in direction and magnitude) to a given metabolic perturbation and 2). the relative proportion of IGF-I sequestered in ternary vs. nonternary molecular complexes appears to be well maintained.

To examine correlates of the speed at which female soldiers carrying loads could cover 3.2 km on foot and traverse an obstacle course, 12 volunteers (mean +/- SD: 25.3 +/- 6 years, 166 +/- 7 cm, 61.3 +/- 7 kg) were timed over 3.2 km while carrying loads of 14, 27, and 41 kg, and while traversing an obstacle course with the two lighter loads. Pearson correlations showed that absolute VO_[2 max] and 3.2 km run time without a load were the best predictors of 3.2 km load carriage time for all loads. Also, larger subjects with greater muscle mass were able to carry the heaviest load faster than smaller, less muscular subjects, likely because the 41 kg load represented a smaller percentage of the former's bodyweight. Maximum number of sit-ups and push-ups, composite score of the Army Physical Fitness Test as well as body height were positively correlated with the speed at which some course segments were traversed.

Dietary creatine (Cr) supplementation has been shown to enhance muscular strength and endurance. This study determined the effects of Cr supplementation on performance of military training tasks. Two groups (Cr and placebo [Pl]) of 13 male soldiers each performed 3 consecutive military obstacle course runs ( approximately 3 minutes over 7 obstacles with a 2-minute rest between runs) followed by a rifle marksmanship task on 3 occasions (T(1), T(2), and T(3)), each separated by 5 days. They also completed a bench press protocol (5 sets to failure at 70% of 1 repetition maximum) and answered the Profile of Mood States questionnaire during each test session. Testing was done 3 times. No supplementation was given before T(1). Supplementation was provided using sports bars, with both groups receiving Pl bars between T(1) and T(2), whereas from T(2) to T(3) the Cr group consumed 24 g per day of Cr monohydrate in sports bars and the Pl group consumed an equal amount (kilocalories) of Pl sports bars. Creatine usage resulted in a significant (14%) increase in total bench press repetitions (p/= 0.05), but no difference between groups in obstacle course run times for the 3 runs from T(2) to T(3). Marksmanship or mood was not affected by Cr supplementation. An increase of 1.4 kg in body mass (p/= 0.005) and a 0.5% decrease in percent body fat (p/= 0.05) were observed after Cr ingestion. Creatine supplementation over 5 days improved performance during a controlled strength test but did not significantly improve military obstacle course performance.

Prior studies have suggested that angiotensin I-converting enzyme (ACE) genotype correlates with superior physical performance in highly selected populations. This study assessed whether such an association exists in a heterogeneous population. Using polymerase chain reaction techniques, we determined the ACE genotypes (insertion/insertion, deletion/insertion, or deletion/deletion) of 62 male and 85 female US Army recruits. Before and after 8 wk of basic training, we determined peak oxygen uptake and performance on the Army Physical Fitness Test (APFT), which includes standardized measures of muscular endurance (sit-ups, push-ups) and a 2-mile run. Subjects of different ACE genotypes had similar peak oxygen uptakes and APFT scores, both before and after training. Subjects with genotype II had higher APFT scores than others, but the differences were not statistically significant. Furthermore, no ACE genotype group had a performance advantage in analyses that adjusted for baseline fitness. We conclude that ACE genotype does not have a strong effect on aerobic power or muscular endurance in healthy, young American adults drawn from an ethnically and geographically diverse population.

Past investigations indicate that training-related injuries are associated with certain performance-oriented measures of physical fitness and certain lifestyle characteristics. This study examined associations between injuries, direct (physiological) measures of physical fitness, and lifestyle characteristics.Subjects were 756 men and 474 women performing the standardized activities involved in U.S. Army Basic Combat Training (BCT). Before BCT, a subsample of subjects (182 men and 168 women) were administered a series of tests that included a treadmill running test (peak VO2), dual-energy x-ray absorptiometry (for body composition), several measures of muscle strength, a hamstring flexibility test, and a vertical jump. A questionnaire addressed smoking habits and prior physical activity. All subjects were administered the Army Physical Fitness test consisting of push-ups, sit-ups, and a 3.2-km run. Gender, age, stature, and body mass were obtained from physical examination records. Injuries incurred during BCT were transcribed from medical records; for each medical visit, the diagnosis, anatomical location, disposition (final outcome of visit), and days of limited duty were recorded.Women had over twice the injury rate of men. For men and women, fewer push-ups, slower 3.2-km run times, lower peak VO2, and cigarette smoking were risk factors for time-loss injury. Among the men only, lower levels of physical activity before BCT and both high and low levels of flexibility were also time-loss injury risk factors. Multivariate analysis revealed that lower peak VO2 and cigarette smoking were independent risk factors for time-loss injury.Lower aerobic capacity and cigarette smoking were independently associated with a higher likelihood of injury in both men and women during a standardized program of physical training. Further studies are needed to assess associations between injury and body composition and muscular strength.

Study objectives To measure the prevalence of exercise-induced bronchospasm (EIB) and to determine its effect on the physical performance response to training in otherwise healthy young adults. Design Observational, retrospective study. Setting Fort Jackson, SC, May to July 1998. Participants One hundred thirty-seven ethnically diverse US Army recruits undergoing an 8-week Army basic training course. Measurements and results Subjects underwent exercise challenge testing at the end of basic training to evaluate for EIB (defined as a decrease in FEV 1 of ≥ 15%, 1 or 10 min after running to peak oxygen uptake on a treadmill). Those subjects who were unable to run to peak oxygen uptake, or who were unable to perform two baseline FEV 1 maneuvers the results of which were within 5% of each other, were excluded from analysis. We measured peak oxygen uptake on a treadmill and the scores achieved on the components of the US Army physical fitness test (APFT). Of 137 subjects, 121 (58 men and 63 women) met our inclusion criteria. Eight subjects (7%) had EIB. Subjects who experienced EIB and unaffected control subjects both showed statistically significant gains in performance on the APFT events during basic training. At the end of basic training, peak oxygen uptake levels and APFT event scores were not significantly different between subjects with EIB and unaffected control subjects. Conclusions Seven percent of the US Army recruits who were tested had EIB, but this did not hinder their physical performance gains during basic training. EIB per se should not be an absolute reason to exclude individuals from employment in jobs with heavy physical demands.

This study examined the effectiveness of basic combat training (BCT) in improving the physical fitness of incoming soldiers and compared the physical fitness and trainability of current trainees to those measured in previous years. 350 soldiers (182 men and 168 women) were recruited from those entering two BCT battalions at the Ft Jackson Reception Station during May 1-14, 1998. Volunteers performed the following procedures before BCT: (1) continuous uphill treadmill running test of peak oxygen uptake (VO2peak); (2) one-repetition maximum (1-RM) isometric strength test of the lower body, upper torso and upright pulling strength; (3) 1-RM test of dynamic lifting strength; (4) dual-energy X-ray absorptiometry (DEXA) assessment of body composition; (5) anthropometric measurements (skinfolds and circumferences); (6) vertical jump; (7) photometric measurement of limb length and joint diameters; and (8) joint mobility measures.

Equine arteritis virus (EAV) is the causative agent of equine viral arteritis, an apparently emerging disease of equids. In this study, the antibody response of horses to the structural proteins of EAV was evaluated using gradient-purified EAV virions and baculovirus-expressed recombinant EAV structural proteins (GL, GS, M, N) as antigens in a Western immunoblotting assay. Thirty-three sera from horses that previously had been naturally or experimentally infected with EAV were evaluated, including samples from mares, geldings, and both persistently and nonpersistently infected stallions. Sera also were evaluated from 4 horses that had been vaccinated with the commercial modified live EAV vaccine. The data suggest that the serologic response of individual horses to EAV may vary with the infecting virus strain and duration of infection. The M protein was most consistently recognized by the various serum samples, whereas the response to the N and GL proteins was variable and the GS protein was bound by only 1 serum sample. The immunoblotting assay definitively established the protein specificity of the humoral response of horses to EAV; however, it clearly is less sensitive than the standard serum neutralization (SN) test—2 of the 37 sera that were serpositive by th SN test failed to react in the immunoblot assay with any EAV structural protein. Furthermore, the GL protein expresses the known neutralization determinants of EAV, yet only 22 of the 37 sera that had SN antibodies bound the GL protein in the immunoblotting assay. Information from this study will assist ongoing efforts to develop improved methods for the serologic diagnosis of EAV infection of horses.

Variations in the density of the fat-free mass (DFFM) across ethnic groups is a critical factor that invalidates the use of body fat equations. It has also been suggested that resistance trained athletes may have higher body densities (BDs) than untrained subjects. Thus, the validity of using anthropometric (ANT) equations, which have mainly been derived on white nonathletic groups, has been questioned for athletic white and black men. This study compared BD and percent body fat (%BF) between 34 white (20 +/- 1 yr, 184 +/- 11 cm, 84 +/- 12 kg, 25 +/- 3 BMI) and 30 black (20 +/- 1 yr, 182 +/- 9 cm, 84 +/- 12 kg, 25 +/- 2 BMI) male collegiate athletes and determined the accuracy of 5 ANT equations in estimating %BF. Subjects were underwater weighed (UWW), and skinfold measurements were obtained from the chest, mid-axillary, abdomen, suprailiac, subscapula, triceps, and thigh. BD was obtained from UWW and estimated from the five skinfold equations. From UWW, significant (P < or = 0.05) differences were found for BD (1.075 +/- 0.007 vs 1.0817 +/- 0.009), but not for %BF (10.49 +/- 2.8 vs 11.59 +/- 3.4) for white and black subjects, respectively. Differences were noted for subcutaneous skinfold sites (abdominal (vertical), suprailiac, and thigh), sum of three and seven skinfolds, and proportion of subscapular subcutaneous fat. One out of five and five out of five ANT equations (Siri conversions) yielded significantly lower estimates compared with UWW %BF for the white and black athletes, respectively. Use of the Schutte equation for the black athletes resulted in overpredictions of %BF for five out of five equations. In addition, the Schutte equation offered slightly greater accuracy than did the Siri equation for estimating %BF in black athletes. These data confirm earlier concerns that ANT equations derived on general populations may not be as accurate for athletic populations and also suggest that correction equations are necessary for converting BD into %BF for populations differing with respect to race or training status.

The gender differences in peak oxygen uptake (VO2peak) for various modes of exercise have been examined previously; however, no direct gender comparisons have been made during repetitive lifting (RL). In the present study the VO2peak between RL and treadmill running (TR) was compared between 20 men [mean (SD) age, height, body mass and body fat: 21 (3) years, 1.79 (0.06) m, 81 (9) kg, 19 (6)%, respectively] and 20 women [mean (SD) age, height, body mass and body fat: 21 (3) years, 1.63 (0.05) m, 60 (7) kg, 27 (6)%, respectively]. VO2peak (l x min[-1]), defined as the highest value obtained during exercise to volitional fatigue, was determined using discontinuous protocols with treadmill grade or box mass incremented to increase exercise intensity. For RL VO2peak, a pneumatically driven shelf was used to lower a loaded box to the floor, and subjects then lifted the box, at a rate of 15 lifts x min(-1). VO2peak (l x min(-1) and ml x kg(-1) x min[-1]) and minute ventilation (VE, l x min[-1]) were determined using an on-line gas analysis system. A two-way repeated measures analysis of variance revealed significant gender effects, with men having higher values for VO2peak (l x min(-1) and ml x kg(-1) x min[-1]) and VE, but women having higher values of the ventilatory equivalent for oxygen (VE/VO2). There were also mode of exercise effects, with TR values being higher for VO2peak (l x min(-1) and ml x kg(-1) x min[-1]) and VE and an interaction effect for VO2peak (l x min(-1) and ml x kg(-1) x min[-1]) and VE/VO2. The women obtained a greater percentage (approximately 84%) of their TR VO2peak during RL than did the men (approximately 79%). There was a marginal tendency for women to decrease and men to increase their VE/VO2 when comparing TR with RL. The magnitude of the gender differences between the two exercise modalities appeared to be similar for heart rate, VE and R, but differed for VO2peak (l x min(-1) and ml x kg(-1) x min[-1]). Lifting to an absolute height (1.32 m for the RL protocol) may present a different physical challenge to men and women with respect to the degree of involvement of the muscle groups used during lifting and ventilation.

The abdomen is the principal site of fat deposition in men, and because abdominal fat is readily mobilized during exercise, the relative proportion of fat in the abdominal site may negatively correlate with the amount of regular physical activity, and even with physical fitness. This study presents data for regional fatness in 165 fit young men (U.S. Army Ranger candidates; initial body fat = 14.7 +/- 4.7%) assessed by dual-energy x-ray absorptiometry (DEXA), and for relative changes occurring following a 13% weight loss produced by a 1000 kcal.d-1 energy deficit over 8 wk. Fat-free mass was constant across quintiles of percent body fat; only fat mass was different (16.2 +/- 2.2 kg and 6.0 +/- 1.4 kg at upper and lower quintiles, respectively). Truncal fat accounted for about 41% of total body fat in all quintiles; only the proportion of fat distributed to the arms was significantly higher in the fattest quintiles of men. Among a group of less intensely trained soldiers with the same average fatness as the highest quintile of Ranger students (20%), relative fat distribution to the trunk approached 50% of the total fat. Following weight loss, Ranger students lost half of the fat in all regions assessed (legs, arms, and trunk). The only significant association between regional losses and initial fatness was a greater proportion of fat lost from the arms in the fattest Rangers. These data suggest a "fit fat" distribution in active young men in which fat remains in the arms and legs until extreme weight loss occurs and the metabolically more active abdominal fat approaches depletion.

Cattle bloods containing only polymerase chain reaction (PCR)-detectable bluetongue-10 viral nucleic acid, but as determined by virus isolation techniques, not bluetongue-10 virus, were incapable of infecting intrathoracically inoculated Culicoides variipennis sonorensis. These insects also failed to transmit bluetongue-10 virus when fed on sheep. Cattle whose blood contain only PCR-detectable bluetongue viral nucleic acid, but no infectious virus, are unlikely to play a role in the epidemiology of bluetongue. The biological significance of PCR-based detection assays and their effect on animal health regulations on the international trade of livestock and livestock germplasm is discussed. Bluetongue virus infection provides a very useful model with which to study arthropod-transmitted RNA virus infections of humans and other animals.

Three adult black-tailed deer (Odocoileus hemionus columbianus) and four fawns were inoculated with bluetongue virus (BTV) serotype 10 or 17, or epizootic hemorrhagic disease virus (EHDV) serotype 1. Animals were bled at irregular intervals thereafter and the presence of virus-specific antibodies in serum determined by agar gel immunodiffusion (AGID), serum neutralization (SN) and competitive enzyme-linked immunosorbent assay (C-ELISA) tests. Serum antibodies to BTV were detected in all three tests for 692 days after inoculation (DAI) of adult deer, but both the SN and AGID tests gave either erroneous or misleading results. Serum from one deer was negative by the AGID test at 409 DAI with BTV-10 but was positive at 248 and 692 DAI; also one adult and one fawn had antibodies by the SN test to serotypes of BTV with which they were not inoculated. The AGID test for EHDV had false positive results with some sera from animals inoculated only with BTV, and it consistently had false negative results with serum samples collected from an EHDV-inoculated deer at 140 DAI and thereafter. The C-ELISA was the most useful test for the detection of antibodies to BTV because it rapidly gave quantitative and accurate results.

Limited information is available regarding the physiological responses to prolonged load carriage. This study determined the energy cost of prolonged treadmill walking (fixed distance of 12 km) at speeds of 1.10 m.s-1, 1.35 m.s-1, and 1.60 m.s-1, unloaded (clothing mass 5.2 kg) and with external loads of 31.5 and 49.4 kg. Fifteen male subjects performed nine trials in random order over a 6-week period. Oxygen uptake (VO2) was determined at the end of the first 10 min and every 20 min thereafter. A 10-min rest period was allowed following each 50 min of walking. No changes occurred in VO2 over time in the unloaded condition at any speed. The 31.5 and 49.4 kg loads, however, produced significant increases (ranging from 10 to 18%) at the two fastest and at all three speeds, respectively, even at initial exercise intensities less than 30% VO2max. In addition, the 49.4 kg load elicited a significantly higher (P less than 0.05) VO2 than did the 31.5 kg load at all speeds. The measured values of metabolic cost were also compared to those predicted using the formula of Pandolf et al. In trials where VO2 increased significantly over time, predicted values underestimated the actual metabolic cost during the final minute by 10-16%. It is concluded that energy cost during prolonged load carriage is not constant but increases significantly over time even at low relative exercise intensities. It is further concluded that applying the prediction model which estimates energy expenditure from short-term load carriage efforts to prolonged load carriage can result in significant underestimations of the actual energy cost.

The objectives of this study were to: (1) determine the distribution of injuries occurring during loaded treadmill marching, (2) determine if there are significant anthropometric changes that occur in the foot during this marching, and (3) identify variables (speed, load) that may affect these injuries and foot changes. This study documented a large number of minor injuries (N equals 82) among physically fit soldiers marching on a treadmill at fast speeds and over a long distance with heavy loads. Significant pre to post foot anthropometric changes (P less than .05) were observed in ball of foot and instep circumferences with a tendency for these changes to occur with the heavier loads while ankle circumference changes occurred throughout most of the conditions. The results of this study indicate that: (1) load carriage injuries occurring during a prolonged treadmill march primarily involve the lower extremities and back, (2) foot blisters were the most common injury recorded, (3) the standard Army practice of using foot powder and undergoing frequent sock changes does not prevent blister formation during prolonged treadmill marches, (4) the most common types of back injury was upper back straining.

The relationship of muscle fiber type and mass to maximal power production and the maintenance of power (endurance time to exhaustion) at 36%, 55%, and 73% of maximal power was investigated in 18 untrained but physically active men. Power output was determined at constant pedalling rate (60 rev.min-1) on a cycle ergometer instrumented with force transducers and interfaced with a computer. Maximal power was determined for each subject as the highest one-revolution average power. Fat-free mass was determined by hydrostatic weighing, fat-free thigh volume by water displacement and skinfold measurement, and percentage and area of type II fibers from biopsy specimens taken from the vastus lateralis. Maximal power averaged 771 +/- 149 W with a range of 527-1125 W. No significant correlations were found among percentage of type II fibers, relative area of type II fibers, or fat-free thigh volume and maximal power or endurance times to exhaustion at any percentage of maximal power. Weak but significant relationships were found for fat-free mass with both maximal power (r = 0.57) and endurance time at 73% of maximal power (r = -0.47). These results show maximal power to be more dependent on factors related to body size than muscle-fiber characteristics. The low correlations for so many of the relationships, however, suggest that individuals employ either different combinations of these factors or utilize other strategies for the generation of high power.

This study examined the cognitive and physiological performance of soldiers as they carried loads over various terrains. Twelve soldiers each carried a light load (total weight, including clothing, 22.77 kg 50.19 LB) and a heavy load (total weight, including clothing, 36.94 kg 81.43 LB) over three terrains: blacktop road, sand, and mud. The cognitive tasks performed by the soldiers included arithmetical, memory, and monitoring tasks. The physiological variables were oxygen uptake, ventilation rate, heart rate, and rating of perceived exertion. Test participants also rated their overall workload after each trial. The results showed a significant (p = .018) Load x Block interaction for the monitoring task. In Block 2, the error rate for the light load condition was significantly lower than the error rate for the heavy load condition. There were significant main effects of load, terrain, and time for all the physiological variables. in this study, the energy expenditure (oxygen uptake) for walking on mud or loose sand was the same, and it was approximately 40% higher than the energy expenditure for walking on the blacktop road. Subjective ratings of workload showed significant differences as a function of load (p = .006) but not terrain.

Seventeen counties in northern California experienced epizootics of high mortality in the mule deer ( Odocoileus hemionus) population during the latter half of 1993. Thirteen deer submitted to the California Veterinary Diagnostic Laboratory System as part of this natural die-off had systemic adenovirus infection. Pulmonary edema was present in all 13 deer. Erosions, ulceration, and abscessation of the upper alimentary tract occurred in 7/13 deer. Four of 13 deer had hemorrhagic enteritis. All 13 deer had widespread systemic vasculitis with endothelial intranuclear inclusions. Fluorescein isothiocyanate-labeled antibody directed against bovine adenovirus type 5 bound to antigen in endothelial cells. Adenovirus was identified by transmission electron microscopy within the nuclei of endothelial cells in 6/6 deer examined. An adenovirus was isolated from lung homogenates of one deer that were cultured on black-tailed deer pulmonary artery endothelial cells. With the exception of the intranuclear inclusions evident on histologic evaluation, gross and histologic changes were similar to those described for bluetongue virus infection and epizootic hemorrhagic disease virus infection in white-tailed deer. Nine additional deer were emaciated and had pharyngeal abscesses with focal vasculitis, which may represent the chronic affects of previous nonfatal adenovirus infection.

This study examined the extent to which aerobic power could account for performance during a 30-s max-effort test. Physically active women (n = 41) and men (n = 34) underwent a treadmill test for aerobic power and the Wingate test for anaerobic power and fatigue. Pearson product-moment correlation coefficients were calculated from Wingate segments of various durations and temporal positions. For aerobic and anaerobic power, all correlations (positive) in both genders were significant (p

Little data exist for upper and lower body mechanical power capability of adolescent athletes. This study compared arm (A) and leg (L) anaerobic peak and mean power (PP and MP) of 20 male and 20 female adolescent athletes after normalization for body mass (BM), fat-free mass (FFM), and lean A and L cross-sectional area (CSA). Power outputs were assessed by the Wingate anaerobic test. FFM and CSA were estimated via anthropometry. No significant (P > 0.05) differences existed between the sexes in Tanner sexual maturity, chronological age, or overall training activity. Males had higher (P < 0.001) absolute PP (W) (L 694 vs 442; A 494 vs 309) and MP (L 548 vs 307; A 337 vs 214). Ratio normalization and ANCOVA were used to remove the influence of body size differences. Ratio normalization showed that males had greater leg PP/BM, MP/BM, MP/FFM, MP/CSA, as well as arm PP/BM and MP/BM, whereas all leg and arm PP and MP ANCOVA adjusted means for BM, FFM, and CSA, except arm MP adjusted for FFM, were significantly (P < 0.01) higher for males than females. We conclude that factors other than muscle mass, possibly qualitative in nature, are responsible for the sex difference in anaerobic performance of adolescent athletes.