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Nanofibrous scaffolds plays a vital role in many biomedical applications such as biosensors, solar cells, membrane filteration, bone regeneration and wound healing applications. In recent decades, around 10-15% of people in India have been facing bone-related problems due to accidents, fractures, loss of bone mass, and aging. To overcome the obstacles, functionalized hybrid biomaterials have been designed to treat or augment bone defects, reduce bacterial infections, and also act as smart drug carriers. The electrospun nanofibers have garnered attention and provides multiple mertis in tissue engineering due to its nano architecture which mimic the micro-structural entity of extracellular matrix (ECM). Fascinatingly, this review examines various electrospinning methods that employs to fabricate nanofibrous scaffold and their effective biomedical applications are explained. The study highlights the experimental set up of electrospinning techniques, it also focuses on recent exploration in emerging biomedical developments. [ABSTRACT FROM AUTHOR]