Search

Your search keyword '"Johan Quintens"' showing total 3 results
Start Over Author "Johan Quintens"
3 results on '"Johan Quintens"'

1. Determination of phage susceptibility as a clinical diagnostic tool: A routine perspective

Author

Valéry Daubie, Houssein Chalhoub, Bob Blasdel, Hafid Dahma, Maya Merabishvili, Tea Glonti, Nathalie De Vos, Johan Quintens, Jean-Paul Pirnay, Marie Hallin, and Olivier Vandenberg

Subjects
phage (bacteriophage), susceptibility, clinical microbiology, diagnosis, personalized medicine, Microbiology, QR1-502
Abstract

As the global burden of disease caused by multidrug resistant bacteria is a major source of concern, credible clinical alternatives to antibiotic therapy, such as personalized phage therapy, are actively explored. Although phage therapy has been used for more than a century, the issue of an easy to implement diagnostic tool for determining phage susceptibility that meets current routine clinical needs is still open. In this Review, we summarize the existing methods used for determining phage activity on bacteria, including the three reference methods: the spot test, the double agar overlay plaque assay, and the Appelmans method. The first two methods rely on the principle of challenging the overnight growth of a lawn of bacteria in an agar matrix to a known relative phage to bacteria concentration and represent good screening tools to determine if the tested phage can be used for a “passive” and or “active” treatment. Beside these methods, several techniques, based on “real-time” growth kinetics assays (GKA) have been developed or are under development. They all monitor the growth of clinical isolates in the presence of phages, but use various detection methods, from classical optical density to more sophisticated techniques such as computer-assisted imagery, flow-cytometry, quantitative real-time polymerase chain reaction (qPCR) or metabolic indicators. Practical considerations as well as information provided about phage activity are reviewed for each technique. Finally, we also discuss the analytical and interpretative requirements for the implementation of a phage susceptibility testing tool in routine clinical microbiology.

Published
2022
Full Text
View/download PDF

2. Oral Intake of Lactobacilli Can Be Helpful in Symptomatic Bacterial Vaginosis: A Randomized Clinical Study

Author

Sergiy Gerasymov, Johan Quintens, Nataliya Henyk, Karl Richir, Ihor Filipiuk, Nataliia Veresniuk, Viktor Maliuk, Tetyana Khyzhnyak, Larysa Zubrytska, Halyna Reznichenko, and Yevhenia Tynna

Subjects
Adult, Limosilactobacillus reuteri, medicine.medical_specialty, Placebo, Gastroenterology, law.invention, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Lactobacillus acidophilus, Lactobacillus rhamnosus, Randomized controlled trial, Anti-Infective Agents, law, Internal medicine, medicine, Humans, Prospective Studies, Prospective cohort study, 030219 obstetrics & reproductive medicine, biology, Lactobacillus crispatus, business.industry, Lacticaseibacillus rhamnosus, Probiotics, Obstetrics and Gynecology, General Medicine, Vaginosis, Bacterial, Middle Aged, biology.organism_classification, medicine.disease, Metronidazole, Treatment Outcome, 030220 oncology & carcinogenesis, Dietary Supplements, Vagina, Female, Bacterial vaginosis, business, medicine.drug
Abstract

Objective The aim of the study was to explore a role of oral intake of a mixture of 3 Lactobacillus species in recurrence of bacterial vaginosis (BV). Materials and methods A phase 2 randomized parallel group prospective placebo-controlled study conducted at 7 clinical centers enrolled 18- to 45-years-old women with recent symptomatic BV cured with metronidazole. Within 48 hours after completion of metronidazole therapy, eligible women received 1 capsule of the verum (5.4 billion Lactobacillus crispatus LMG S-29995, Lactobacillus brevis, and Lactobacillus acidophilus in proportion of 60%, 20%, and 20%, respectively), or the placebo supplement 2 times daily for the first 7 days and 1 time daily for the next 8 to 120 days. The primary outcome measure was the percentage of recurrence of BV, which was defined as 3 of 4 Amsel criteria plus abnormal vaginal discharge/vulvar odor during 4 months of intake of the test dietary supplement. Differences between the groups were assessed with Z test for proportions. Results One hundred sixty-six women were analyzed in the verum (82 patients) and the placebo group (82 patients). Recurrence of BV was documented in 15 (18.3%) of 82 women in the verum group and 27 (32.1%) of 84 in the placebo group (p = .014). Rates of survival without BV rates were higher in the verum group (Cox F test, p = .018). Both verum and placebo supplements were well tolerated. Conclusions Oral intake of L. crispatus LMG S-29995, L. brevis, and L. acidophilus can significantly decrease percent of recurrences of BV in recently treated women and prolong time to recurrence of the disease.

Published
2020

3. Phage Therapy in Europe: Regulatory and Intellectual Property Protection Issues

Author

Gilbert Verbeken, Daniel De Vos, J.-P. Pirnay, and Johan Quintens

Subjects
Clinical trial, Risk analysis (engineering), Phage therapy, Application protocol, Paradigm shift, medicine.medical_treatment, medicine, Business, Intellectual property
Abstract

Bacteriophages (phages for short) are increasingly put forward as potential (additional) tools in the fight against the worldwide antibiotic crisis. However, the setup of clinical trials, which are necessary to demonstrate the efficacy of phages and to optimize their clinical application protocols, and consequently to their implementation in the clinic is still hindered by the lack of an adapted regulatory framework. Although some (local) progresses have been made in recent years, no real paradigm shift has occurred. This chapter discusses the current status in Europe with regard to the two most pertinent barriers to phage therapy: the regulatory and intellectual property protection issues.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results