11 results on '"Joelle Cabon"'
Search Results
2. Potential of Marine Strains of Pseudoalteromonas to Improve Resistance of Juvenile Sea Bass to Pathogens and Limit Biofilm Development
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Alexandra Rahmani, Leila Parizadeh, Marine Baud, Yoannah Francois, Alexis Bazire, Sophie Rodrigues, Yannick Fleury, Helena Cuny, Eric Debosse, Joelle Cabon, Lenaïg Louboutin, Laure Bellec, Morgane Danion, and Thierry Morin
- Abstract
The European sea bass (Dicentrarchus labrax) is one of the most produced marine fish species in Europe. Its larval stage is particularly sensitive and is thus characterized by relatively low survival rates, making it acutely vulnerable to multiple infectious hazards that can occur during its intensive production cycle in hatcheries and at sea. In this study, we investigated the potential probiotic effect of marine Pseudoalteromonas bacterial strains against two major pathogens of this species, Vibrio harveyi (a bacterial pathogen) and the nervous necrosis virus (NNV); we also investigated antibiofilm effect of these Pseudoalteromonas strains. Over an 8 to 12-week impregnation phase, seabass juveniles were immersed fortnightly for 4 hours in static hyperoxygenated seawater containing the probiotic candidates of Pseudoalteromonas strains at a concentration of 106 CFU/mL. We tested four candidates: (1) a combination of two strains producing antimicrobial compounds, hCg-42 and hOe-125; (2) strain 3J6, with known antibiofilm properties and (3) strain RA15, from the same genus, but with no identified probiotic effect. At the end of the impregnation phase, fish underwent an infection challenge with an intraperitoneal injection at a dose of 4.6 × 108 CFU/mL of V. harveyi or a 4-hour bath with a pathogenic strain of NNV. Thereafter, mortality was monitored for 2 and 6 weeks, respectively, at temperatures allowing the development of clinical signs. Immunological analyses were carried out during impregnation and after infection. The probiotic candidates were detected at different sampling times after the 4-hour immersion session in the gills and mucus, but there was no evidence of long-term persistence. For the V. harveyi challenge, no statistical difference in mortality was observed between the non-impregnated control (63%) and the 3J6-impregnated (68.7%) group, but improved survival rates of 10 and 25% were obtained for the RA15- and the double strain (hCg-42+hOe-125)-impregnated groups, respectively. For the NNV challenge, no significant benefic effect of the probiotics on infection kinetics or cumulative mortality was observed. Leucocyte mortality and phagocytosis activity revealed only slight significant differences between the treatment groups, either during impregnation or after infection challenges. Regarding biofilm development during impregnation with probiotic candidates, the maximal thickness of biofilm was significantly lower in the 3J6, double-strain and RA15 groups, compared with the non-impregnated control group. This study highlights the interesting probiotic potential of marine bacteria to limit mortalities induced by bacterial pathogens as well as biofilm development. Further investigations are in progress to investigate the mechanisms of action of these probiotics and to improve their formulation for larger-scale tests.
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- 2023
3. FORTIOR Genetics, une plate-forme pour améliorer la résistance aux maladies des poissons d'élevage par sélection génétique
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Yoannah, Joelle Cabon, Thierry Morin, Daniel Guemene, Pierrick Haffray, Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Laboratoire de Ploufragan-Plouzané-Niort [ANSES], and Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)
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[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2019
4. Heritabilities of resistance to VNN and vibriosis and genetic correlations with production traits and processing yields in two selected commercial lines of the European sea bass Dicentrarchus labrax
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Romain Morvezen, Anastasia Bestin, Anne-Sophie Tiriau, Laurane Pallandre, Joelle Cabon, Yoannah François, Sophie Cariou, Jean-Sébastien Bruant, Céline Coulombet, Aline Bajek, Marc Vandeputte, François Allal, Thierry Morin, Daniel Guemene, Pierrick Haffray, Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Ferme Marine du Douhet, Ecloserie Marine de Gravelines, MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Génétique Animale et Biologie Intégrative (GABI), and AgroParisTech-Institut National de la Recherche Agronomique (INRA)
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[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,[SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies ,[SDV.SA.STP]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of fishery ,[SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2018
5. The relationships between growth rate and mitochondrial metabolism varies over time
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Jean-Baptiste Quéméneur, Morgane Danion, Joëlle Cabon, Sophie Collet, José-Luis Zambonino-Infante, and Karine Salin
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Medicine ,Science - Abstract
Abstract Mitochondrial metabolism varies significantly between individuals of the same species and can influence animal performance, such as growth. However, growth rate is usually determined before the mitochondrial assay. The hypothesis that natural variation in mitochondrial metabolic traits is linked to differences in both previous and upcoming growth remains untested. Using biopsies to collect tissue in a non-lethal manner, we tested this hypothesis in a fish model (Dicentrarchus labrax) by monitoring individual growth rate, measuring mitochondrial metabolic traits in the red muscle, and monitoring the growth of the same individuals after the mitochondrial assay. Individual variation in growth rate was consistent before and after the mitochondrial assay; however, the mitochondrial traits that explained growth variation differed between the growth rates determined before and after the mitochondrial assay. While past growth was correlated with the activity of the cytochrome c oxidase, a measure of mitochondrial density, future growth was linked to mitochondrial proton leak respiration. This is the first report of temporal shift in the relationship between growth rate and mitochondrial metabolic traits, suggesting an among-individual variation in temporal changes in mitochondrial traits. Our results emphasize the need to evaluate whether mitochondrial metabolic traits of individuals can change over time.
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- 2022
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6. Characterization of a New Toti-like Virus in Sea Bass, Dicentrarchus labrax
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Lénaïg Louboutin, Joëlle Cabon, Véronique Beven, Edouard Hirchaud, Yannick Blanchard, and Thierry Morin
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sea bass ,mortality ,genome description ,sea bass toti-like virus (SBTLV) ,epidemiology ,Microbiology ,QR1-502 - Abstract
The European sea bass Dicentrarchus labrax is the main species reared in Mediterranean aquaculture. Its larval stage, which is very sensitive and highly affected by sanitary and environmental conditions, is particularly scrutinized in hatcheries. Recently, a Mediterranean sea bass farm had to deal with an abnormal increase in mortality, especially between 20 and 35 days post-hatching (dph). Biological investigations led to the observation of cytopathic effects on three different fish cell lines after almost 3 weeks of culture at 14 °C in contact with homogenized affected larvae, suggesting the presence of a viral agent. High-throughput sequencing revealed a 6818-nucleotide-long RNA genome with six putative ORFs, corresponding to the organization of viruses belonging to the Totiviridae family. This genome clustered with the newly described and suggested Pistolvirus genus, sharing 45.5% to 37.2% nucleotide identity with other piscine toti-like viruses such as Cyclopterus lumpus toti-like virus (CLuTLV) or piscine myocarditis virus (PMCV), respectively. Therefore, we propose to name this new viral agent sea bass toti-like virus (SBTLV). Specific real-time RT-PCR confirmed the presence of the viral genome in the affected larval homogenate from different production batches and the corresponding cell culture supernatant. Experimental infections performed on sea bass fingerlings did not induce mortality, although the virus could be detected in various organs and a specific immune response was developed. Additional studies are needed to understand the exact involvement of this virus in the mortality observed in hatcheries and the potential associated cofactors.
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- 2023
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7. The extensive transgenerational transcriptomic effects of ocean acidification on the olfactory epithelium of a marine fish are associated with a better viral resistance
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Mishal Cohen-Rengifo, Morgane Danion, Anne-Alicia Gonzalez, Marie-Laure Bégout, Alexandre Cormier, Cyril Noël, Joëlle Cabon, Thomas Vitré, Felix C. Mark, and David Mazurais
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Anti-viral immunity ,Betanodavirus ,Climate change ,European sea bass ,Long-term transgenerational ocean acidification ,Metabolism ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Progressive CO2-induced ocean acidification (OA) impacts marine life in ways that are difficult to predict but are likely to become exacerbated over generations. Although marine fishes can balance acid–base homeostasis efficiently, indirect ionic regulation that alter neurosensory systems can result in behavioural abnormalities. In marine invertebrates, OA can also affect immune system function, but whether this is the case in marine fishes is not fully understood. Farmed fish are highly susceptible to disease outbreak, yet strategies for overcoming such threats in the wake of OA are wanting. Here, we exposed two generations of the European sea bass (Dicentrarchus labrax) to end-of-century predicted pH levels (IPCC RCP8.5), with parents (F1) being exposed for four years and their offspring (F2) for 18 months. Our design included a transcriptomic analysis of the olfactory rosette (collected from the F2) and a viral challenge (exposing F2 to betanodavirus) where we assessed survival rates. Results We discovered transcriptomic trade-offs in both sensory and immune systems after long-term transgenerational exposure to OA. Specifically, RNA-Seq analysis of the olfactory rosette, the peripheral olfactory organ, from 18-months-old F2 revealed extensive regulation in genes involved in ion transport and neuronal signalling, including GABAergic signalling. We also detected OA-induced up-regulation of genes associated with odour transduction, synaptic plasticity, neuron excitability and wiring and down-regulation of genes involved in energy metabolism. Furthermore, OA-exposure induced up-regulation of genes involved in innate antiviral immunity (pathogen recognition receptors and interferon-stimulated genes) in combination with down-regulation of the protein biosynthetic machinery. Consistently, OA-exposed F2 challenged with betanodavirus, which causes damage to the nervous system of marine fish, had acquired improved resistance. Conclusion F2 exposed to long-term transgenerational OA acclimation showed superior viral resistance, though as their metabolic and odour transduction programs were altered, odour-mediated behaviours might be consequently impacted. Although it is difficult to unveil how long-term OA impacts propagated between generations, our results reveal that, across generations, trade-offs in plastic responses is a core feature of the olfactory epithelium transcriptome in OA-exposed F2 offspring, and will have important consequences for how cultured and wild fish interacts with its environment.
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- 2022
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8. The Viral Hemorrhagic Septicemia Virus (VHSV) Markers of Virulence in Rainbow Trout (Oncorhynchus mykiss)
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Laury Baillon, Emilie Mérour, Joëlle Cabon, Lénaïg Louboutin, Estelle Vigouroux, Anna Luiza Farias Alencar, Argelia Cuenca, Yannick Blanchard, Niels Jørgen Olesen, Valentina Panzarin, Thierry Morin, Michel Brémont, and Stéphane Biacchesi
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novirhabdovirus ,viral hemorrhagic septicemia virus ,VHSV ,rainbow trout ,virulence markers ,Microbiology ,QR1-502 - Abstract
Viral hemorrhagic septicemia virus (VHSV) is a highly contagious virus leading to high mortality in a large panel of freshwater and marine fish species. VHSV isolates originating from marine fish show low pathogenicity in rainbow trout. The analysis of several nearly complete genome sequences from marine and freshwater isolates displaying varying levels of virulence in rainbow trout suggested that only a limited number of amino acid residues might be involved in regulating the level of virulence. Based on a recent analysis of 55 VHSV strains, which were entirely sequenced and phenotyped in vivo in rainbow trout, several amino acid changes putatively involved in virulence were identified. In the present study, these amino acid changes were introduced, alone or in combination, in a highly-virulent VHSV 23–75 genome backbone by reverse genetics. A total of 35 recombinant VHSV variants were recovered and characterized for virulence in trout by bath immersion. Results confirmed the important role of the NV protein (R116S) and highlighted a major contribution of the nucleoprotein N (K46G and A241E) in regulating virulence. Single amino acid changes in these two proteins drastically affect virus pathogenicity in rainbow trout. This is particularly intriguing for the N variant (K46G) which is unable to establish an active infection in the fins of infected trout, the main portal of entry of VHSV in this species, allowing further spread in its host. In addition, salmonid cell lines were selected to assess the kinetics of replication and cytopathic effect of recombinant VHSV and discriminate virulent and avirulent variants. In conclusion, three major virulence markers were identified in the NV and N proteins. These markers explain almost all phenotypes (92.7%) observed in trout for the 55 VHSV strains analyzed in the present study and herein used for the backward validation of virulence markers. The identification of VHSV specific virulence markers in this species is of importance both to predict the in vivo phenotype of viral isolates with targeted diagnostic tests and to improve prophylactic methods such as the development of safer live-attenuated vaccines.
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- 2020
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9. VHSV Single Amino Acid Polymorphisms (SAPs) Associated With Virulence in Rainbow Trout
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Valentina Panzarin, Argelia Cuenca, Michele Gastaldelli, Anna L. F. Alencar, Francesco Pascoli, Thierry Morin, Yannick Blanchard, Joëlle Cabon, Lénaïg Louboutin, David Ryder, Miriam Abbadi, Anna Toffan, Carlos P. Dopazo, Stéphane Biacchesi, Michel Brémont, and Niels J. Olesen
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VHSV ,molecular markers ,single amino acid polymorphism (SAP) ,virulence ,rainbow trout ,Microbiology ,QR1-502 - Abstract
The Viral Hemorrhagic Septicemia Virus (VHSV) is an OIE notifiable pathogen widespread in the Northern Hemisphere that encompasses four genotypes and nine subtypes. In Europe, subtype Ia impairs predominantly the rainbow trout industry causing severe rates of mortality, while other VHSV genotypes and subtypes affect a number of marine and freshwater species, both farmed and wild. VHSV has repeatedly proved to be able to jump to rainbow trout from the marine reservoir, causing mortality episodes. The molecular mechanisms regulating VHSV virulence and host tropism are not fully understood, mainly due to the scarce availability of complete genome sequences and information on the virulence phenotype. With the scope of identifying in silico molecular markers for VHSV virulence, we generated an extensive dataset of 55 viral genomes and related mortality data obtained from rainbow trout experimental challenges. Using statistical association analyses that combined genetic and mortality data, we found 38 single amino acid polymorphisms scattered throughout the complete coding regions of the viral genome that were putatively involved in virulence of VHSV in trout. Specific amino acid signatures were recognized as being associated with either low or high virulence phenotypes. The phylogenetic analysis of VHSV coding regions supported the evolution toward greater virulence in rainbow trout within subtype Ia, and identified several other subtypes which may be prone to be virulent for this species. This study sheds light on the molecular basis for VHSV virulence, and provides an extensive list of putative virulence markers for their subsequent validation.
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- 2020
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10. Genome-wide association and genomic prediction of resistance to viral nervous necrosis in European sea bass (Dicentrarchus labrax) using RAD sequencing
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Christos Palaiokostas, Sophie Cariou, Anastasia Bestin, Jean-Sebastien Bruant, Pierrick Haffray, Thierry Morin, Joëlle Cabon, François Allal, Marc Vandeputte, and Ross D. Houston
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Animal culture ,SF1-1100 ,Genetics ,QH426-470 - Abstract
Abstract Background European sea bass (Dicentrarchus labrax) is one of the most important species for European aquaculture. Viral nervous necrosis (VNN), commonly caused by the redspotted grouper nervous necrosis virus (RGNNV), can result in high levels of morbidity and mortality, mainly during the larval and juvenile stages of cultured sea bass. In the absence of efficient therapeutic treatments, selective breeding for host resistance offers a promising strategy to control this disease. Our study aimed at investigating genetic resistance to VNN and genomic-based approaches to improve disease resistance by selective breeding. A population of 1538 sea bass juveniles from a factorial cross between 48 sires and 17 dams was challenged with RGNNV with mortalities and survivors being recorded and sampled for genotyping by the RAD sequencing approach. Results We used genome-wide genotype data from 9195 single nucleotide polymorphisms (SNPs) for downstream analysis. Estimates of heritability of survival on the underlying scale for the pedigree and genomic relationship matrices were 0.27 (HPD interval 95%: 0.14-0.40) and 0.43 (0.29–0.57), respectively. Classical genome-wide association analysis detected genome-wide significant quantitative trait loci (QTL) for resistance to VNN on chromosomes (unassigned scaffolds in the case of ‘chromosome’ 25) 3, 20 and 25 (P
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- 2018
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11. FORTIOR Genetics, a platform to enhance disease resistance by genetic selection in aquaculture
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Yoannah, Joelle Cabon, Thierry Morin, Daniel Guemene, Pierrick Haffray, Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Laboratoire de Ploufragan-Plouzané-Niort [ANSES], and Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)
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[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
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