Search

Control of musculoskeletal systems depends on integration of voluntary commands and somatosensory feedback in the complex neural circuits of the spinal cord. It has been suggested that the various connectivity patterns that have been identified experi- mentally may result from the many transcriptional types that have been observed in spinal interneurons. We ask instead whether the muscle-specific details of observed connectivity patterns can arise as a consequence of Hebbian adaptation during early de- velopment, rather than being genetically ordained. We constructed an anatomically simplified model musculoskeletal system with realistic muscles and sensors and connected it to a recurrent, random neuronal network consisting of both excitatory and inhibitory neurons endowed with Hebbian learning rules. We then generated a wide set of randomized muscle twitches typical of those described during fetal development and allowed the network to learn. Multiple simulations consistently resulted in diverse and stable patterns of activity and connectivity that included subsets of the interneurons that were similar to “archetypi- cal” interneurons described in the literature. We also found that such learning led to an increased degree of cooperativity between interneurons when performing larger limb movements on which it had not been trained. Hebbian learning gives rise to rich sets of diverse interneurons whose connectivity reflects the mechanical properties of the system. At least some of the tran- scriptomic diversity may reflect the effects of this process rather than the cause of the connectivity. Such a learning process seems better suited to respond to the musculoskeletal mutations that underlie the evolution of new species.

Recent spinal cord literature abounds with descriptions of genetic preprogramming and the molecular control of circuit formation. In this paper, we explore to what extent circuit formation based on learning rather than preprogramming could explain the selective formation of the monosynaptic projections between muscle spindle primary afferents and homony- mous motoneurons. We adjusted the initially randomized gains in the neural network according to a Hebbian plasticity rule while exercising the model system with spontaneous muscle activity patterns similar to those observed during early fetal development. Normal connectivity patterns developed only when we modeled b motoneurons, which are known to innervate both intrafusal and extrafusal muscle fibers in vertebrate muscles but were not considered in previous literature regarding selective formation of these synapses in animals with paralyzed muscles. It was also helpful to correctly model the greatly reduced contractility of extrafusal muscle fibers during early development. Stronger and more coordinated muscle activity patterns such as observed later during neonatal locomotion impaired projection selectivity. These findings imply a generic functionality of a musculoskeletal system to imprint important aspects of its mechanical dynamics onto a neural network, without specific preprogramming other than setting a critical period for the formation and maturation of this general pattern of connectivity. Such functionality would facilitate the successful evolution of new species with altered musculoskeletal anatomy, and it may help to explain patterns of connectivity and associated reflexes that appear during abnormal development.

The spinal cord is engaged in all forms of motor performance but its functions are far from understood. Because network connectivity defines function, we explored the connectivity of muscular, tendon, and tactile sensory inputs among a wide population of spinal interneurons in the lower cervical segments. Using low noise intracellular whole cell recordings in the decerebrated, non-anesthetized cat

Manuscript "A Non-spiking Neuron Model With Dynamic Leak to Avoid Instability in Recurrent Networks"

The mammalian brain could contain dense and sparse network connectivity structures, including both excitatory and inhibitory neurons, but is without any clearly defined output layer. The neurons have time constants, which mean that the integrated network structure has state memory. The network structure contains complex mutual interactions between the neurons under different conditions, which depend on the internal state of the network. The internal state can be defined as the distribution of activity across all individual neurons across the network. Therefore, the state of a neuron/network becomes a defining factor for how information is represented within the network. Towards this study, we constructed a fully connected (with dense/sparse coding strategies) recurrent network comprising of both excitatory and inhibitory neurons, driven by pseudo-random inputs of varying frequencies. In this study we assessed the impact of varying specific intrinsic parameters of the neurons that enriched network state dynamics, such as initial neuron activity, amount of inhibition in combination with thresholded neurons and conduction delays. The impact was assessed by quantifying the changes in mutual interactions between the neurons within the network for each given input. We found such effects were more profound in sparsely connected networks than in densely connected networks. However, also densely connected networks could make use of such dynamic changes in the mutual interactions between neurons, as a given input could induce multiple different network states., Comment: 13 pages, 5 figures