13 results on '"João L.S.G. Vianez"'
Search Results
2. Congenital Zika virus infection impairs corpus callosum development
- Author
-
Átila Duque Rossi, Amilcar Tanuri, Luiza M. Higa, Raiane Oliveira Ferreira, Roberto Lent, Daniel M. Guimarães, Jéssica de Cassia Cavalheiro Gomes Ferreira, Patricia P. Garcez, João L.S.G. Vianez, Janaina M. Vasconcelos, Bruna Valério-Gomes, Jefferson H. Quintanilha, Raissa R. Christoff, and Maria Bellio
- Subjects
Pathology ,medicine.medical_specialty ,Microcephaly ,Neurogenesis ,Biology ,medicine.disease ,Corpus callosum ,biology.organism_classification ,Axonogenesis ,Zika virus ,medicine.anatomical_structure ,nervous system ,medicine ,Axon guidance ,Axon ,Ventriculomegaly - Abstract
Congenital Zika Syndrome (CZS) is a set of birth defects caused by Zika virus (ZIKV) infection during pregnancy. Microcephaly is its main feature, but other brain abnormalities are found in CZS patients, such as ventriculomegaly, brain calcifications, and dysgenesis of the corpus callosum. Many studies have focused on microcephaly, but it remains unknown how ZIKV infection leads to callosal malformation. To tackle this issue, we infected mouse embryos in utero with a Brazilian ZIKV isolate and found that they are born with a reduction in callosal area and density of callosal neurons. ZIKV infection also causes a density reduction of PH3+ cells, intermediate progenitor cells and SATB2+ neurons. Moreover, axonal tracing revealed that callosal axons are reduced and misrouted. Also, ZIKV infected cultures show a reduction of callosal axon length. GFAP labelling showed that in utero infection compromises glial cells responsible for midline axon guidance. The RNA-Seq data from infected brains identified downregulation of axon guidance and axonogenesis related genes. In sum, we showed that ZIKV infection impairs critical steps of corpus callosum formation by disrupting not only neurogenesis but also axon guidance and growth across the midline.Summary StatementZika virus infection during development impairs the formation of corpus callosum by disturbing axon guidance and growth of callosal neurons.
- Published
- 2021
3. Analysis of Kojic Acid Derivatives as Competitive Inhibitors of Tyrosinase: A Molecular Modeling Approach
- Author
-
Kauê Santana da Costa, Clauber Henrique Souza da Costa, Renan Valente, Cláudio Nahum Alves, Richelly Cardoso, Fábio Alberto de Molfetta, and João L.S.G. Vianez
- Subjects
Skin Neoplasms ,Molecular model ,Tyrosinase ,Pharmaceutical Science ,Organic chemistry ,01 natural sciences ,Analytical Chemistry ,Levodopa ,chemistry.chemical_compound ,QD241-441 ,Catalytic Domain ,Drug Discovery ,Enzyme Inhibitors ,Melanoma ,Malononitrile ,0303 health sciences ,biology ,Molecular Structure ,skin cancer ,Monophenol Monooxygenase ,Molecular Docking Simulation ,Chemistry (miscellaneous) ,Melan?citos ,Molecular Medicine ,Melanocytes ,melanogenesis ,kojic acid derivatives ,Stereochemistry ,Monofenol Mono-Oxigenase ,Molecular Dynamics Simulation ,tyrosinase ,Article ,Tirosina / uso terap?utico ,03 medical and health sciences ,Structure-Activity Relationship ,Biosynthesis ,Humans ,Chelation ,Physical and Theoretical Chemistry ,Histidine ,030304 developmental biology ,Melanins ,010405 organic chemistry ,Active site ,molecular docking ,?cido K?jico / utiliza??o ,molecular dynamics ,0104 chemical sciences ,chemistry ,Neoplasias Cut?neas ,Pyrones ,biology.protein ,Tyrosine ,Kojic acid - Abstract
This research received financial support of CAPES and CNPq. Universidade Federal do Par?. Instituto de Ci?ncias Exatas e Naturais. Laborat?rio de Modelagem Molecular. Bel?m, PA, Brasil / Universidade Federal do Par?. Instituto de Ci?ncias Exatas e Naturais. Laborat?rio de Planejamento e Desenvolvimento de F?rmacos. Bel?m, PA, Brasil. Universidade Federal do Par?. Instituto de Ci?ncias Exatas e Naturais. Laborat?rio de Sistemas Moleculares Complexos. Bel?m, PA, Brasil. Universidade Federal do Par?. Instituto de Ci?ncias Exatas e Naturais. Laborat?rio de Planejamento e Desenvolvimento de F?rmacos. Bel?m, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Universidade Federal do Par?. Instituto de Ci?ncias Exatas e Naturais. Laborat?rio de Planejamento e Desenvolvimento de F?rmacos. Bel?m, PA, Brasil / Universidade Federal do Oeste do Par?. Instituto de Biodiversidade. Santar?m, PA, Brasil Universidade Federal do Par?. Instituto de Ci?ncias Exatas e Naturais. Laborat?rio de Modelagem Molecular. Bel?m, PA, Brasil. Universidade Federal do Par?. Instituto de Ci?ncias Exatas e Naturais. Laborat?rio de Planejamento e Desenvolvimento de F?rmacos. Bel?m, PA, Brasil. Abstract: Tyrosinases belong to the functional copper-containing proteins family, and their structure contains two copper atoms, in the active site, which are coordinated by three histidine residues. The biosynthesis of melanin in melanocytes has two stages depending on the actions of the natural substrates L-DOPA and L-tyrosine. The dysregulation of tyrosinase is involved in skin cancer initiation. In the present study, using molecular modeling tools, we analyzed the inhibition activity of tyrosinase activity using kojic acid (KA) derivatives designed from aromatic aldehydes and malononitrile. All derivatives showed conformational affinity to the enzyme active site, and a favorable distance to chelate the copper ion, which is essential for enzyme function. Molecular dynamics simulations revealed that the derivatives formed promising complexes, presenting stable conformations with deviations between 0.2 and 0.35 ?. In addition, the investigated KA derivatives showed favorable binding free energies. The most stable KA derivatives showed the following binding free energies: ?17.65 kcal mol?1 (D6), ?18.07 kcal mol?1 (D2), ?18.13 (D5) kcal mol?1, and ?10.31 kcal mol?1 (D4). Our results suggest that these derivatives could be potent competitive inhibitors of the natural substrates of L-DOPA (?12.84 kcal mol?1) and L-tyrosine (?9.04 kcal mol?1) in melanogenesis
- Published
- 2021
- Full Text
- View/download PDF
4. Congenital Zika syndrome is associated with maternal protein malnutrition
- Author
-
Paula N. Gonzalez, Átila Duque Rossi, C. K. N. Amorim, Zoltán Molnár, J. C. C. G. Ferreira, Patricia P. Garcez, Flávio Alves Lara, Renato S. Aguiar, Jimena Barbeito-Andrés, F. F. Dutra, R. O. Ferreira, Janaina Mota de Vasconcelos, Marcia C. Castro, Leila Chimelli, Claudio Gustavo Barbeito, João L.S.G. Vianez, R. V. Barbosa, Paula Pezzuto, M. P. C. De Souza, Maria Bellio, Ricardo Tadeu Lopes, Amilcar Tanuri, Patricia Cuervo, Marcelo T. Bozza, Luiza M. Higa, T. M. P. Santos, and Acp Dias
- Subjects
Microcephaly ,Epidemiology ,Biología ,Physiology ,Zika virus ,Disease Outbreaks ,purl.org/becyt/ford/1 [https] ,0302 clinical medicine ,Pregnancy ,BRAIN DEVELOPMENT ,Research Articles ,2. Zero hunger ,0303 health sciences ,Multidisciplinary ,Zika Virus Infection ,Brain ,Gene Expression Regulation, Developmental ,SciAdv r-articles ,Defici?ncia de Prote?na ,Organ Size ,Syndrome ,Viral Load ,3. Good health ,Female ,Viral load ,Brazil ,Research Article ,medicine.medical_specialty ,Offspring ,Medicina ,Neurogenesis ,Biology ,03 medical and health sciences ,Microcefalia ,Virology ,medicine ,Diet, Protein-Restricted ,Animals ,IMMUNOHISTOCHEMISTRY ,purl.org/becyt/ford/1.6 [https] ,MORPHOMETRICS ,030304 developmental biology ,Gravidez / gen?tica ,Body Weight ,Malnutrition ,Outbreak ,medicine.disease ,biology.organism_classification ,Embryo, Mammalian ,Zika virus / patogenicidade ,Mice, Inbred C57BL ,Animals, Newborn ,030217 neurology & neurosurgery ,Neuroscience - Abstract
Zika virus (ZIKV) infection during pregnancy is associated with a spectrum of developmental impairments known as congenital Zika syndrome (CZS). The prevalence of this syndrome varies across ZIKV endemic regions, suggesting that its occurrence could depend on cofactors. Here, we evaluate the relevance of protein malnutrition for the emergence of CZS. Epidemiological data from the ZIKV outbreak in the Americas suggest a relationship between undernutrition and cases of microcephaly. To experimentally examine this relationship, we use immunocompetent pregnant mice, which were subjected to protein malnutrition and infected with a Brazilian ZIKV strain. We found that the combination of protein restriction and ZIKV infection leads to severe alterations of placental structure and embryonic body growth, with offspring displaying a reduction in neurogenesis and postnatal brain size. RNA-seq analysis reveals gene expression deregulation required for brain development in infected low-protein progeny. These results suggest that maternal protein malnutrition increases susceptibility to CZS., La lista completa de autores que integran el documento puede consultarse en el archivo., Facultad de Ciencias Veterinarias
- Published
- 2020
5. Low Prevalence of HIV-1 Integrase Resistance Among Antiretroviral-Naive Patients Newly Diagnosed with HIV-1 from Belém, Pará, Amazon Region of Brazil
- Author
-
Susan D Flores Irias, Ana Beatriz Figueiredo de Lima, Regiane S.K. Frânces, Luiz Fernando Almeida Machado, Clayton Pereira Silva de Lima, Poliana da Silva Lemos, Maria Karoliny da Silva Torres, Maria E.S. Avelino, Danilo Leôncio Aguiar Pereira, Renato R.M. Oliveira, Mike S. Barbosa, and João L.S.G. Vianez
- Subjects
Adult ,Male ,Anti-HIV Agents ,Immunology ,Human immunodeficiency virus (HIV) ,HIV Infections ,HIV Integrase ,Newly diagnosed ,medicine.disease_cause ,Young Adult ,Virology ,Drug Resistance, Viral ,Prevalence ,Antiretroviral naive ,Humans ,Medicine ,Amazon rainforest ,business.industry ,Infectious Diseases ,Mutation ,HIV-1 ,Hiv 1 integrase ,Female ,business ,Brazil - Published
- 2020
6. Comparative analysis of the transcriptome of the Amazonian fish species Colossoma macropomum (tambaqui) and hybrid tambacu by next generation sequencing
- Author
-
Horacio Schneider, Iracilda Sampaio, João L.S.G. Vianez, Jedson Ferreira Cardoso, Márcio Roberto Teixeira Nunes, Clayton Pereira Silva de Lima, Luciana Watanabe, and Fátima Gomes
- Subjects
0106 biological sciences ,Fish species ,Ontologia Gen?tica ,Gene Expression ,Muscle Proteins ,Aquaculture ,Tambaqui ,Biochemistry ,01 natural sciences ,Transcriptome ,Caraciformes / gen?tica ,Medicine and Health Sciences ,Musculoskeletal System ,Colossoma macropomum ,0303 health sciences ,Multidisciplinary ,Gene Ontologies ,Muscles ,High-Throughput Nucleotide Sequencing ,Agriculture ,Genomics ,Peixes / gen?tica ,Medicine ,Fish Farming ,Metabolic Pathways ,Anatomy ,Characiformes ,Manejo de Esp?cimes ,Transcriptome Analysis ,Research Article ,Fish farming ,Science ,Muscle Tissue ,Fisheries ,Biology ,010603 evolutionary biology ,DNA sequencing ,03 medical and health sciences ,Genetics ,Animals ,030304 developmental biology ,Biology and Life Sciences ,Computational Biology ,Proteins ,Genome Analysis ,biology.organism_classification ,Fishery ,Aquicultura ,Biological Tissue ,Metabolism ,Skeletal Muscles - Abstract
Center of Technological Innovation (CIT), Evandro Chagas Institute (IEC) and National Council of Scientific and Technological Development (CNPq) (Bionorte Project: 550939/2010-5). Universidade Federal do Par? - Campus de Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. Universidade Federal do Par? - Campus de Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em Virologia. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Universidade Federal do Par? - Campus de Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. Universidade Federal do Par? - Campus de Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. BACKGROUND: The C. macropomum is a characiform fish from the Amazon basin that has been hybridized with other pacu species to produce commercial hybrids, such as the tambacu. However, little is known of the functional genomics of the parental species or these hybrid forms. The transcriptome of C. macropomum and tambacu were sequenced using 454 Roche platform (pyrosequencing) techniques to characterize the domains of Gene Ontology (GO) and to evaluate the levels of gene expression in the two organisms. RESULTS: The 8,188,945 reads were assembled into 400,845 contigs. A total of 58,322 contigs were annotated with a predominance of biological processes for both organisms, as determined by Gene Ontology (GO). Similar numbers of metabolic pathways were identified in both the C. macropomum and the tambacu, with the metabolism category presenting the largest number of transcripts. The BUSCO analysis indicated that our assembly was more than 40% complete. We identified 21,986 genes for the two fishes. The P and Log2FC values indicated significant differences in the levels of gene expression, with a total of 600 up-regulated genes. CONCLUSION: In spite of the lack of a reference genome, the functional annotation was successful, and confirmed a considerable difference in the specificity and levels of gene expression between the two organisms. This report provides a comprehensive baseline for the genetic management of these commercially important fishes, in particular for the identification of specific genes that may represent markers involved in the immunity, growth, and fertility of these organisms, with potential practical applications in aquaculture management.
- Published
- 2019
7. Zika virus complete genome from Salvador, Bahia, Brazil
- Author
-
Poliana da Silva Lemos, Fernando Neto Tavares, Massimo Ciccozzi, Nuno R. Faria, Márcio Roberto Teixeira Nunes, João L.S.G. Vianez, Darren P. Martin, Janaina Mota de Vasconcelos, Sandro Patroca da Silva, Marta Giovanetti, Mateus Santana do Rosário, Tulio de Oliveira, Oliver G. Pybus, Sueli Rodrigues, Luiz Carlos Junior Alcantara, Isadora Cristina de Siqueira, and José Lourenço
- Subjects
0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,Virus transmission ,030231 tropical medicine ,Genome, Viral ,Microbiology ,Genetic analysis ,Genome ,Brasil / epidemiologia ,Zika virus ,03 medical and health sciences ,0302 clinical medicine ,Phylogenetics ,Genoma Viral ,Genetics ,medicine ,Humans ,Zika virus / isolamento & purifica??o ,Molecular Biology ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,biology ,Zika Virus Infection ,Public health ,Outbreak ,Zika Virus ,Middle Aged ,biology.organism_classification ,Zika virus / gen?tica ,Virology ,Filogenia ,030104 developmental biology ,Infectious Diseases ,Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos ,Female ,Brazil - Abstract
Funda??o Oswaldo Cruz. Salvador, BA, Brazil / University of Rome ?Tor Vergata?. Roma, Italy. University of Oxford. Department of Zoology. Oxford, UK. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. University of Oxford. Department of Zoology. Oxford, UK. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. University of Cape Town. Faculty of Health Sciences. Division of Computational Biology. Cape Town, South Africa. Funda??o Oswaldo Cruz. Salvador, BA, Brazil / Hospital Santa Isabel. Salvador, BA, Brazil. Funda??o Oswaldo Cruz. Salvador, BA, Brazil. National Institute of Healthy. Rome, Italy. University of Oxford. Department of Zoology. Oxford, UK. Hospital Santa Isabel. Salvador, BA, Brazil. Funda??o Oswaldo Cruz. Salvador, BA, Brazil. In May 2015 the first autochthonous Zika virus infection was reported in Brazil. Rapid and urgent measures are needed to contain the ongoing outbreak. Here we report the full-length ZIKV coding sequence from Bahia. Genetic analysis of outbreak sequences will be essential for characterizing the diversity of circulating strains, identifying hotspots of virus transmission and guiding public health control. Rapid and urgent measures are needed to contain the ongoing outbreak.
- Published
- 2016
8. De novo transcriptome based on next-generation sequencing reveals candidate genes with sex-specific expression in Arapaima gigas (Schinz, 1822), an ancient Amazonian freshwater fish
- Author
-
Clayton Pereira Silva de Lima, Fátima Gomes, Iracilda Sampaio, Jedson Ferreira Cardoso, Luciana Watanabe, João L.S.G. Vianez, Márcio Roberto Teixeira Nunes, and Horacio Schneider
- Subjects
0106 biological sciences ,0301 basic medicine ,Male ,Serum Proteins ,Skin Neoplasms ,ved/biology.organism_classification_rank.species ,De novo transcriptome assembly ,Cell Membranes ,lcsh:Medicine ,Gene Expression ,01 natural sciences ,Biochemistry ,Transcriptome ,Medicine and Health Sciences ,lcsh:Science ,Skin Tumors ,Sex Characteristics ,Multidisciplinary ,Bacia Amaz?nica (BR) ,Pigmentation ,Liver Diseases ,Gene Ontologies ,Fishes ,High-Throughput Nucleotide Sequencing ,Genomics ,Oncology ,Liver ,Female ,Anatomy ,Cellular Structures and Organelles ,Transcriptome Analysis ,Research Article ,Transcriptoma / gen?tica ,Caracteres Sexuais ,Dermatology ,Gastroenterology and Hepatology ,Biology ,010603 evolutionary biology ,Sequenciamento Completo do Genoma / m?todos ,DNA sequencing ,03 medical and health sciences ,Pirarucu ,Arapaima ,Peixes / anatomia & histologia ,Genetics ,Animals ,Blast2GO ,ved/biology ,Gene Expression Profiling ,lcsh:R ,Biology and Life Sciences ,Computational Biology ,Cancers and Neoplasms ,Proteins ,Membrane Proteins ,Molecular Sequence Annotation ,Cell Biology ,biology.organism_classification ,Genome Analysis ,Sexual dimorphism ,Fatty Liver ,030104 developmental biology ,Evolutionary biology ,Pyrosequencing ,lcsh:Q ,Arapaima gigas - Abstract
The study was funded by the Center of Technological Innovation (CIT), Evandro Chagas Institute (IEC) and National Council of Scientific and Technological Development (CNPq) (Bionorte Project: 550939/2010-5) Universidade Federal do Par? - Campus de Bragan?a. Instituto de Estudos Costeiros. Laborat?rio de Gen?tica e Biologia Molecular. Bragan?a, PA, Brazil. Universidade Federal do Par? - Campus de Bragan?a. Instituto de Estudos Costeiros. Laborat?rio de Gen?tica e Biologia Molecular. Bragan?a, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Universidade Federal do Par? - Campus de Bragan?a. Instituto de Estudos Costeiros. Laborat?rio de Gen?tica e Biologia Molecular. Bragan?a, PA, Brazil. Universidade Federal do Par? - Campus de Bragan?a. Instituto de Estudos Costeiros. Laborat?rio de Gen?tica e Biologia Molecular. Bragan?a, PA, Brazil. BACKGROUND: The Arapaima (Arapaima gigas) is one of the world's largest freshwater bony fish, and is found in the rivers of the Amazon basin. This species is a potential aquaculture resource, although reproductive management in captivity is limited in particular due to the lack of external sexual dimorphism. In this study, using the 454 Roche platform (pyrosequencing) techniques, we evaluated a major portion of the transcriptome of this important Amazonian species. RESULTS: Four libraries obtained from the liver and skin tissue of juvenile specimens (representing males and females separately) were sequenced, yielding 5,453,919 high-quality reads. The de novo transcriptome assembly resulted in 175,792 contigs, with 51,057 significant blast hits. A total of 38,586 transcripts were mapped by Gene Ontology using Blast2GO. We identified 20,219 genes in the total transcriptome (9,551 in the liver and 16,818 in the skin). The gene expression analyses indicated 105 genes in the liver and 204 in the skin with differentiated expression profiles, with 95 being over-expressed in the females and 214 in the males. The log2 Fold Change and heatmap based on Reads Per Kilobase per Million mapped reads (RPKM) revealed that the gene expression in the skin is highly differentiated between male and female arapaima, while the levels of expression in the liver are similar between the sexes. CONCLUSION: Transcriptome analysis based on pyrosequencing proved to be a reliable tool for the identification of genes with differentiated expression profiles between male and female arapaima. These results provide useful insights into the molecular pathways of sexual dimorphism in this important Amazonian species, and for comparative analyses with other teleosts
- Published
- 2018
9. New virus genome sequences of the Guama Serogroup (Genus Orthobunyavirus, Family Bunyaviridae), isolated in the Brazilian Amazon Region
- Author
-
Sueli Guerreiro Rodrigues, Márcio Roberto Teixeira Nunes, Sandro Patroca da Silva, João L.S.G. Vianez, Valéria Lima Carvalho, Davi Toshio Inada, Clayton Pereira Silva de Lima, Daniele Barbosa de Almeida Medeiros, Jedson Ferreira Cardoso, and Pedro Fernando da Costa Vasconcelos
- Subjects
0301 basic medicine ,Arbovirus ,Amazon rainforest ,viruses ,Bunyaviridae ,030231 tropical medicine ,Family Bunyaviridae ,Biology ,Genome ,Virology ,Regi?o Amaz?nica (BR) ,Virus ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,parasitic diseases ,Viruses ,Genetics ,Genoma Viral ,Genus Orthobunyavirus ,Molecular Biology - Abstract
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. This is the first announcement of two nearly complete viral genome sequences belonging to the Guama serogroup (genus Orthobunyavirus, family Bunyaviridae) isolated in the Brazilian Amazon region: Mirim virus (MIRV; BEAN7722) and Ananindeua virus (ANUV; BEAN109303).
- Published
- 2017
10. Identification and characterization of the expression profile of the microRNAs in the Amazon species Colossoma macropomum by next generation sequencing
- Author
-
Fátima Gomes, Márcio Roberto Teixeira Nunes, Sérgio Ricardo Nozawa, Iracilda Sampaio, João L.S.G. Vianez, Horacio Schneider, Jedson Ferreira Cardoso, Luciana Watanabe, and Layanna Freitas de Oliveira
- Subjects
0301 basic medicine ,Gene regulatory network ,An?lise de Sequ?ncia de RNA ,Computational biology ,Biology ,Biologia Computacional ,DNA sequencing ,03 medical and health sciences ,Sequenciamento de Nucleot?deos em Larga Escala ,Caraciformes / gen?tica ,Ecossistema Amaz?nico (BR) ,MicroRNAs / gen?tica ,Peixes / anatomia & histologia ,microRNA ,Genetics ,Animals ,Gene ,Caraciformes / metabolismo ,Skin ,Regulation of gene expression ,Peixes ,Amazon rainforest ,business.industry ,Sequence Analysis, RNA ,Pele / metabolismo ,Computational Biology ,High-Throughput Nucleotide Sequencing ,Ecossistema Amaz?nico ,F?gado / metabolismo ,Biotechnology ,Transcriptoma ,MicroRNAs ,030104 developmental biology ,Functional annotation ,Liver ,Identification (biology) ,Characiformes ,business ,Transcriptome ,Express?o G?nica - Abstract
Federal University of Par? - Campus of Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. Federal University of Par? - Campus of Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. Dow AgroSciences. Ribeir?o Preto, SP, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Federal University of Par? - Campus of Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. Federal University of Par? - Campus of Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. Colossoma macropomum is a resistant species native of Amazonas and Orinoco river basins. It is regarded as the second largest finfish of Solim?es and Amazon rivers, representing a major fishery resource in Amazonas and an important species in tropical aquaculture. MicroRNAs are non-coding endogenous riboregulators of nearly 22 nucleotides that play a key role in post-transcriptional gene regulation of several organisms. We analyzed samples of liver and skin from specimens of C. macropomum using next generation sequencing. The dataset was evaluated using computational programs to check the quality of sequences, identification of miRNAs, as well as to evaluate the expression levels of these microRNAs and interaction of target genes. We identified 279 conserved miRNAs, being 257 from liver and 272 from skin, with several miRNAs shared between tissues, with divergence in the number of reads. The strands miR-5p and miR-3p were observed in 72 miRNAs, some of them presenting a higher number of 3p reads. The functional annotation of the most expressed miRNAs resulted in 27 pathways for the liver and skin mainly related to the ?biological processes? domain. Based on the identified pathways, we visualized a large gene network, suggesting the regulation of selected miRNA over this interactive dataset. We were able to identify and characterize the expression levels of miRNAs in two tissues of great activity in C. macropomum, which stands out as the beginning of several studies that can be carried out to elucidate the influence of miRNAs in this species and their applicability as biotechnological tools.
- Published
- 2017
11. Isolation of Madre de Dios Virus (Orthobunyavirus; Bunyaviridae), an Oropouche Virus Species Reassortant, from a Monkey in Venezuela
- Author
-
Humberto Montañez, Rosa Hernandez, Juan Carlos Navarro, Scott C. Weaver, Jonathan Liria, Márcio Roberto Teixeira Nunes, Janaina M. Vasconcelos, Albert J. Auguste, Jorge Fernando Soares Travassos da Rosa, Rodrigo Santos de Oliveira, Sandro Patroca da Silva, Robert B. Tesh, João L.S.G. Vianez, Anderson Lima, and Dileyvic Giambalvo
- Subjects
0301 basic medicine ,Orthobunyavirus ,030106 microbiology ,Bunyaviridae Infections ,Genome, Viral ,medicine.disease_cause ,Cercopithecus aethiops ,Virus ,03 medical and health sciences ,Virology ,Reassortant Viruses ,Chlorocebus aethiops ,medicine ,Animals ,Cebus ,Vero Cells ,Phylogeny ,biology ,Phylogenetic tree ,Oropouche virus ,High-Throughput Nucleotide Sequencing ,Articles ,biology.organism_classification ,Venezuela ,030104 developmental biology ,Infectious Diseases ,RNA, Viral ,Parasitology ,Bunyaviridae - Abstract
Oropouche virus (OROV), genus Orthobunyavirus, family Bunyaviridae, is an important cause of human illness in tropical South America. Herein, we report the isolation, complete genome sequence, genetic characterization, and phylogenetic analysis of an OROV species reassortant, Madre de Dios virus (MDDV), obtained from a sick monkey (Cebus olivaceus Schomburgk) collected in a forest near Atapirire, a small rural village located in Anzoategui State, Venezuela. MDDV is one of a growing number of naturally occurring OROV species reassortants isolated in South America and was known previously only from southern Peru.
- Published
- 2015
12. Analysis of a Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) for yellow fever diagnostic
- Author
-
Robert B. Tesh, Keley N. B Nunes, Lívia Carício Martins, Sandro Patroca da Silva, Márcio Roberto Teixeira Nunes, Valéria Lima Carvalho, Hilda Guzman, Clayton Pereira Silva de Lima, Pedro Fernando da Costa Vasconcelos, and João L.S.G. Vianez
- Subjects
Molecular Sequence Data ,Context (language use) ,Human pathogen ,Biology ,Viral Nonstructural Proteins ,Genome ,Virus ,Virology ,Yellow Fever ,medicine ,V?rus da Febre Amarela / isolamento & purifica??o ,Humans ,Diagnostic ,Reverse Transcription Loop-mediated Isothermal Amplification ,RT-LAMP ,DNA Primers ,Febre Amarela / virologia ,Base Sequence ,Yellow fever ,Outbreak ,Central America ,Nucleic acid amplification technique ,South America ,medicine.disease ,Genome detection ,T?cnicas de Amplifica??o de ?cido Nucleico / m?todos ,Caribbean Region ,Population Surveillance ,Africa ,Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos ,Febre Amarela / diagn?stico ,Transcri??o Reversa ,Yellow fever virus ,Nucleic Acid Amplification Techniques - Abstract
This study was partially supported by CNPq (Conselho Nacional para o Desenvolvimento Cient?fico e Tecnol?gico) projects 401558/2013-4; 457664/2013-4, 486069/2012-5 and 301641/2010-2), and CNPq/CAPES/FAPESPA (project 573739-2008-0). Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / University of Para State. Department of Pathology. Belem, PA, Brazil. University of Texas Medical Branch. Department of Pathology. Galveston, TX, USA. University of Texas Medical Branch. Department of Pathology. Galveston, TX, USA. Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinct techniques based on the RT-PCR derived method have been previously developed, however both methods are expensive due to the use of thermo cyclers and labeled probes. We developed isothermal genome amplification, which is a rapid, sensitive, specific and low cost molecular approach for YFV genome detection. This assay used a set of degenerate primers designed for the NS1 gene and was able to amplify, within 30 min in isothermal conditions, the YFV 17D vaccine strain derived from an African wild prototype strain (Asibi),as well as field strains from Brazil, other endemic countries from South and Central America, and the Caribbean. The generic RT-LAMP assay could be helpful for YFV surveillance in field and rapid response during outbreaks in endemic areas.
- Published
- 2015
13. Emergence and potential for spread of Chikungunya virus in Brazil
- Author
-
Eliana Vieira Pinto da Silva, Jedson Ferreira Cardoso, Moritz U. G. Kraemer, Simon I. Hay, Pedro Fernando da Costa Vasconcelos, Márcio Roberto Teixeira Nunes, Layanna Freitas de Oliveira, João L.S.G. Vianez, Nuno R. Faria, Janaina Mota de Vasconcelos, Sandro Patroca da Silva, Bruno Tardelli Diniz Nunes, Robert B. Tesh, Raimunda do Socorro da Silva Azevedo, Oliver G. Pybus, Ana Cecília Ribeiro Cruz, Daisy Elaine Andrade da Silva, Sueli Guerreiro Rodrigues, Giovanini E. Coelho, Nick Golding, and Valéria Lima Carvalho
- Subjects
Adult ,Male ,Risk ,medicine.medical_specialty ,Aedes albopictus ,Statistical methods ,Adolescent ,Genotype ,medicine.disease_cause ,Disease Outbreaks ,Young Adult ,Epidemiology ,medicine ,Humans ,Chikungunya ,Child ,Index case ,Phylogeny ,Aged ,Medicine(all) ,Public health ,biology ,business.industry ,Transmission (medicine) ,Infant, Newborn ,Outbreak ,virus diseases ,Infant ,General Medicine ,Middle Aged ,biology.organism_classification ,Virology ,3. Good health ,Spatial prediction ,Vector (epidemiology) ,Child, Preschool ,National surveillance ,Chikungunya Fever ,Female ,business ,Chikungunya virus ,Brazil ,Research Article - Abstract
Background In December 2013, an outbreak of Chikungunya virus (CHIKV) caused by the Asian genotype was notified in the Caribbean. The outbreak has since spread to 38 regions in the Americas. By September 2014, the first autochthonous CHIKV infections were confirmed in Oiapoque, North Brazil, and in Feira de Santana, Northeast Brazil. Methods We compiled epidemiological and clinical data on suspected CHIKV cases in Brazil and polymerase-chain-reaction-based diagnostic was conducted on 68 serum samples from patients with symptom onset between April and September 2014. Two imported and four autochthonous cases were selected for virus propagation, RNA isolation, full-length genome sequencing, and phylogenetic analysis. We then followed CDC/PAHO guidelines to estimate the risk of establishment of CHIKV in Brazilian municipalities. Results We detected 41 CHIKV importations and 27 autochthonous cases in Brazil. Epidemiological and phylogenetic analyses indicated local transmission of the Asian CHIKV genotype in Oiapoque. Unexpectedly, we also discovered that the ECSA genotype is circulating in Feira de Santana. The presumed index case of the ECSA genotype was an individual who had recently returned from Angola and developed symptoms in Feira de Santana. We estimate that, if CHIKV becomes established in Brazil, transmission could occur in 94% of municipalities in the country and provide maps of the risk of importation of each strain of CHIKV in Brazil. Conclusions The etiological strains associated with the early-phase CHIKV outbreaks in Brazil belong to the Asian and ECSA genotypes. Continued surveillance and vector mitigation strategies are needed to reduce the future public health impact of CHIKV in the Americas. Electronic supplementary material The online version of this article (doi:10.1186/s12916-015-0348-x) contains supplementary material, which is available to authorized users.
- Published
- 2015
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.