Search

Colorectal cancer (CRC) is a common malignant tumor with strong invasiveness. Given the reported involvement of DEP domain-containing protein 1B (DEPDC1B) in the progression of some cancers, its role in CRC was explored in this study. DEPDC1B expression in CRC was assessed based on database and tissue microarray (TMA). In addition, the knockdown and overexpression of DEPDC1B in CRC cell lines were constructed using small hairpin RNA (shRNA) interference. The biological function of DEPDC1B in CRC was evaluated in vitro and in vivo through loss/gain-of-function assays. The results demonstrated that DEPDC1B was highly expressed in CRC. Furthermore, DEPDC1B had the ability to promote CRC proliferation and migration coupled by cell apoptosis.

In an uncooled infrared imaging system, thermal radiation effects are caused by the heat source from the target or the detection window, which affects the ability of target detection, tracking, and recognition seriously. To address this problem, a multi-scale correction method via a fast surface fitting with Chebyshev polynomials is proposed. A high-precision Chebyshev polynomial surface fitting is introduced into thermal radiation bias field estimation for the first time, to the best of our knowledge. The surface fitting in the gradient domain is added to the thermal radiation effects correction model as a regularization term, which overcomes the ill-posed matrix problem of high-order bivariate polynomials surface fitting, and achieves higher accuracy under the same order. Additionally, a multi-scale iterative strategy and vector representation are adopted to speed up the iterative optimization and surface fitting, respectively. Vector representation greatly reduces the number of basis function calls and achieves fast surface fitting. In addition, split Bregman optimization is used to solve the minimization problem of the correction model, which decomposes the multivariable optimization problem into multiple univariate optimization sub-problems. The experimental results of simulated and real degraded images demonstrate that our proposed method performs favorably against the state of the art in thermal radiation effects correction.

The threaded rod without water stop plate is used to replace the original water stop bolt. The threaded rod is composed of a full-length threaded rod, a tapered screw sleeve and a nut. With the help of the threaded rod, the water seepage path of the wall is changed, the water seepage line is extended, and the water pressure is reduced, so as to achieve the water stop effect. Its implementation not only reduces the processing difficulty, but also can adjust the bolt buckle spacing according to the thickness of the outer wall of the basement, effectively strengthen the formwork, and reduce the risk of formwork expansion and explosion; It can be manually removed to reduce on-site hot work. It is a better choice for the basement wall formwork system.

Erectile dysfunction (ED) is a major health problem affecting a large proportion of the general population. Testosterone also plays a key role in sexual dysfunction. In this study, we found that testosterone can inhibit cavernous fibrosis by affecting the expression of miR-22-3p, providing a new basis for research and treatment of ED. Old and young rats were used to study the effects of testosterone on cavernous fibrosis. Hematoxylin and eosin (HE) and Masson's staining were used to observe the cavernous tissue. A luciferase assay was used to analyze the relationship between the miR-22-3p, TGFβR1, and Galectin-1 signaling pathways. CCK-8 and flow cytometry were used to detect the proliferation and apoptosis rates of cavernosum smooth muscle cells (CSMCs) following testosterone intervention. Immunohistochemical analysis was performed to examine the positive rate of caspase 3 and Ki67. IF was used to analyze the expression of collagen IV, MMP2, and α-SMA. The levels of GnRH, tT, LH, and F-TESTO in old rats increased after testosterone intervention. miR-22-3p inhibits the expression of TGFβR1 and Galectin-1. The protein expression of TGFβR1, Galectin-1, SMAD2, and p-SMAD2 was reduced by testosterone. The expression levels of α-SMA, collagen I, collagen IV, FN, and MMP2 in the cavernous tissues of old rats treated with testosterone were significantly reduced. The levels of caspase 3 and collagen IV decreased, and the levels of MMP2, Ki67, and α-SMA increased. Testosterone and miR-22-3p inhibit CSMC apoptosis and promote cell proliferation. Testosterone promoted the expression of miR-22-3p to interfere with the expression of the cavernous TGFβR1 and Galectin-1 signaling pathways. Testosterone can reduce cavernous fibrosis during the treatment of functional ED.

To explore the protective effect of rosiglitazone (RGZ) on hepatic ischemia reperfusion injury (HIRI) and the underlying mechanisms. Methods: A rat model of ischemia-reperfusion injury was established by clamping the left and middle lobe of liver with noninvasive vascular clamp. A total of 30 Sprague-Dawley rats were randomly divided into a sham group, an HIRI group, and a RGZ group (10 rats in each group). Two hours after reperfusion, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, lactate dehydrogenase (LDH) level, malondialdehyde (MDA) content and catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were examined. HE staining was used to observe liver pathological morphology. The liver peroxisome proliferators-activated receptor γ (PPAR-γ), p-PPAR-γ, nuclear factor related factor 2 (Nrf-2), antioxidant response element (ARE), heme oxygenase 1 (HO-1) and quinone oxidoreductase-1 (NQO-1) were detected by Western blot. Results: Compared with the HIRI group, the levels of ALT, AST, LDH and MDA in the RGZ group were significantly decreased (all P0.05), while the levels of Nrf-2, ARE, HO-1 and NQO-1 in the RGZ group were significantly increased. The hepatic swelling, necrosis and pathological damage were decreased (all P0.05). In addition, there was no difference in the level of PPAR-γ between the 2 groups (P0.05). Conclusion: PPAR-γ agonist RGZ can attenuate HIRI, which may be related to activating Nrf2/ARE signaling pathway and enhancement of antioxidant ability.目的：探讨罗格列酮(rosiglitazone，RGZ)对肝缺血再灌注损伤(hepatic ischemia reperfusion injury，HIRI)的保护作用及其相关机制。方法：用无创血管夹夹闭左、中叶肝蒂构建70%HIRI大鼠模型。30只Sprague-Dawley大鼠随机均分为假手术组、HIRI组和RGZ组，每组10只。再灌注2 h后，检测血清中谷丙转氨酶(alanine aminotransferase，ALT)、谷草转氨酶(aspartate aminotransferase，AST)、乳酸脱氢酶(lactate dehydrogenase，LDH)表达水平，以及肝丙二醛(malondialdehyde，MDA)的含量和过氧化氢酶(content and catalase，CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase，GPx)、超氧化物歧化酶(superoxide dismutase，SOD)的活性；HE染色检测肝病理形态；Western印迹检测肝过氧化物酶体增殖物激活受体-γ(peroxisome proliferators-activated receptor γ，PPAR-γ)、磷酸化修饰的PPAR-γ(p-PPAR-γ)、核因子相关因子2(nuclear factor related factor 2，Nrf-2)、抗氧化反应元件(antioxidant response element，ARE)、血红素氧化酶1(heme oxygenase 1，HO-1)、还原型辅酶/醌氧化还原酶-1(quinone oxidoreductase-1，NQO-1)表达量。结果：与HIRI组相比，RGZ组ALT，AST，LDH 和MDA表达水平均明显降低(均P0.05)，而p-PPAR-γ，Nrf-2，ARE，HO-1和NQO-1表达水平，以及CAT，GPX和SOD活性均明显增高(均P0.05)，此外，肝组织肿胀和坏死以及病理损伤均明显减轻(均P0.05)，而PPAR-γ表达水平差异无统计学意义(P0.05)。结论：PPAR-γ激动剂RGZ能减轻肝HIRI，其作用途径可能与激活Nrf2/ARE信号途径而增强机体抗氧化能力有关。.

A fiber-optic low-coherent interferometry sensor was developed to measure the ground settlement (GS) in an accuracy of the micrometer. The sensor combined optical techniques with liquid-contained chambers that were hydraulically connected together at the bottom by using a water-filled tube. The liquid surface inside each chamber was at the same level initially. The optical interferometry was employed to read out the liquid level changes, which following the GS happened at the place where the chamber was put on and, thereby, the GS information was calculated. The laboratory effort had demonstrated its potential in the practical application. Here, the denoising algorithms on the measurement signal were carried out based on the specific environment to ensure the accuracy and stability of the system in field applications. After that, we extended this technique to the high-speed railway. The 5-days continuous measurement proved that the designed system could be applied to monitor the GS of the high-speed railway piers and approached an accuracy of ±70 μm in the field situation with a reference compensation sensor. So the performance of the sensor was suitable to the GS monitoring problem in the high-speed railway. There, the difficulties were to meet the monitoring requirement of both a large span in space and its quite tiny and slow changes.

Objective: To investigate the correlation between circulating endothelial cells (CECs) and vascular lesions in renal allografts. Methodology: Sixty-two renal transplant patients were divided into four groups according to biopsy data. CECs were isolated from peripheral blood with anti-CD136-coated immunomagnetic Dynabeads and counted by microscopy during biopsy. CEC numbers were compared in each group, as well as the correlation between CECs and C4d and vascular changes in different groups. Result: CECs counts were higher in the acute rejection (AR) with endarteritis group than in the normal group (p < 0.01), acute tubular necrosis (ATN) group (p < 0.01) and chronic allograft nephropathy (CAN) group (p < 0.01), there were no difference among ATN, normal and CAN) group (p = 0.587). There was no difference among the normal group without hyaline, normal group with hyaline and CAN with hyaline group. An increasing CECs count was related to C4d-positive AR (p = 0.008; κ score = 0.519) and infiltration of inflammatory cells (p = 0.002, κ score = 0.573) in proximal tubule cells (PTCs). The CECs count decreased after intensive therapy in five patients (p = 0.001). Conclusion: Elevation of the CEC count in blood was related to endarteritis. Elevation of CEC count was related to C4d deposition and infiltration of inflammatory cells in PTCs.

Traditional statistical approaches for identifying multi-word terms have to handle a large amount of noisy data and are extremely time consuming. This paper presents a new statistical approach for identifying multiword terms based on the co-related text-segments existing in a group of documents. The approach involves three stages: (a) using a short predefined stoplist as an initial input to segment a set of text documents into text-segments, (b) calculating the segment-weights of all text-segments, and (c) applying the short text-segments to segment the longer text-segments based on the weight values. The newly generated text-segments then segment each other again until all text-segments cannot be further divided. The resultant text-segments are identified as terms based on a specified threshold. The initial experimental result on a set of traditional Chinese documents shows that this approach can achieve a minimum of 76.39% of recall rate and a minimum of 91.05% of precision rate on retrieving multiple occurrences terms, including 18.30% of new identified terms. The approach can be applied to identify multi-word terms in any languages.

Traditional statistical approaches for identifying multi-word terms have to handle a large amount of noisy data and are extremely time consuming. This paper introduces a multi-word term extraction system for extracting multiword terms from a set of documents based on the co-related text-segments existing in these documents. The system uses a short predefined stoplist as an initial input to segment a set of documents into text-segments, calculates the segment-weights of all text-segments, and then applies the short text-segments to segment the longer text-segments based on the weight values recursively until all text-segments cannot be further divided. The resultant text-segments can thus be identified as terms based on a specified threshold. The initial experimental result on a set of traditional Chinese documents shows that this system can achieve a minimum of 76.39% of recall rate and a minimum of 91.05% of precision rate on retrieving multiple occurrences terms, which include 18.30% of new identified terms.

Parallel Web documents are the crucial lexical basis for constructing robust multilingual Web-based linguistic knowledge resources. To identify parallel Web documents efficiently and effectively, this paper develops a new automatic approach based on filenames using the commonly used parallel document naming practice on the Web. The approach involves three procedures for identifying common file descriptor, language flag, and language flag-pair respectively among all file names examined. To examine how these three procedures can be used to get the best result, five methods are developed by incorporating these procedures in different ways. An experimental study on a Hong Kong government Web site is conducted to evaluate the performance of these five methods in terms of recall and precision. The experimental result shows that the method combining the procedures of the file descriptor alignment and the language flag-pair alignment outperforms other methods, with a 95.3% of precision rate and a 91.0% of recall rate.

The current systems for the support of online learning provide a certain degree of multimedia capability by allowing users to access online learning objects but are still limited when accessing the actual content of learning objects and exchanging experiences about the learning objects. The work introduced in this paper describes an innovative multimedia tool for enriched communication between users and highly flexible control of the use of learning objects.