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Gully erosion is one of the most severe types of land degradation, hindering food production and sustainable agricultural development. However, the historical evolution process and the impact of land use change on gully erosion remain unclear. To address this issue, we conducted a field investigation on gully erosion in 2018 and interpreted land use and gullies using historical remote sensing images in 1968 and 1978 over an area of 84.48 km2. The study found that from 1968 to 1978 to 2018, all gully morphological parameters including gully length density and gully areal density increased significantly. The main origin of gully erosion found was from dry farmland. The annual soil loss rate induced by gully erosion was 1.46 mm during 1968–2018. Gully erosion rates were higher during 1968–1978 than during 1978–2018. Furthermore, the length, areal and volumetric erosion rates in gullies formed by multiple gullies merging was greater than that of newly formed gullies (NFG) and gullies developing continuously from a single pre-existing gully, while the widening rate of NFG was highest. The susceptibility of land use types to gully erosion was in the order of woodland

Royal jelly (RJ) is rich in various nutrients, including proteins and amino acids. RJ consumption improves sexual and urinary function in postmenopausal women, possibly owing to its hormone-like effects. Currently, hormone regulation and assisted reproductive technologies are widely used to treat thin endometria; however, high doses of these drugs can add to liver burden. In this study, RJ proteins and fatty acids were isolated, and their therapeutic effects on thin endometria were investigated in vivo and in vitro to verify their hormone-like regulatory effects. The results showed that RJ and its extracts could regulate endometrial receptivity-related factor expression levels in rats with a thin endometrium, accelerate MCF-7 cell apoptosis, and increase estrogen receptor expression in MCF-7 cells wherein. The estrogen-like effects of RJ and the fatty acids may be the main contributors. RJ may be a potential natural hormone replacement therapy.

During freeze–thaw cycling, aggregates undergo a dynamic change in breakdown–formation (turnover), however, how the turnover occurs between aggregates of various particle sizes is not clear. To clarify the influence of freeze–thaw cycling on the dynamic changes in the particle size of soil aggregates, soil aggregates from the Black Soil Region of Northeast China were selected as the research objects. The study conducted in situ dynamic monitoring experiments, innovatively applying the rare earth oxide (REO) tracer method to natural conditions of freeze–thaw cycles (autumn freeze–thaw period, freezing period, and spring freeze–thaw period), accurately tracking the turnover paths and quantifying the turnover rates between aggregates of various particle sizes. The results revealed that the total value of the formation paths of the 2–5 mm aggregates and 0.25–2 mm aggregates increased during the autumn freeze–thaw period. The number of freeze–thaw cycles and accumulated snowfall were significantly positively correlated with aggregate stability, with an increase in the number of freeze–thaw cycles and accumulated snowfall resulting in an increase in the proportion of aggregates > 0.25 mm, which improved aggregate stability. In addition, the total value of the breakdown path of macro-aggregates increased during the spring freeze–thaw cycling period. Soil moisture was significantly negatively correlated with aggregate stability, with increased soil moisture resulting in a decrease in the percentage of aggregates > 0.25 mm, which resulted in a decrease in aggregate stability. The study can provide a reference understanding for the effects of freeze–thaw cycles on the structure of black soil and provide a theoretical basis for improving the quality of arable land.

Abstract In seasonal frozen soil, freezing and thawing can change the physical and mechanical properties and affect slope stability. There are complex moisture conditions in the main water transfer canal. A study of the hydrothermal evolution of canals with different initial water contents under the action of freezing and thawing is of great importance for the prevention and control of canal slope slides. Hydrothermal coupling models are the key to revealing the canal's hydrothermal evolution. As some of the modeling parameters in the current hydrothermal coupling model are based on empirical values, particularly those in the van Genuchten equation, which are not necessarily related to soil properties, they are not suitable for analyzing the hydrothermal evolution of canals. This paper determines the soil‐water characteristic curve from the cumulative curve of particle gradation in the subsoil, and then determines the hydraulic parameters of the subsoil using the VG model, which then corrects the hydrothermal coupling model. The method of modifying the hydrothermal coupling model is original, which makes the model more realistically reflect drainage soil characteristics. During freezing and thawing of channel slopes with different initial water contents (21%, 25%, 29%, 33%, 37%, and 41%), temperature field, water field, and ice content distributions were investigated. Using the V‐G model, the optimal parameters for canal subsoil were a = 0.06, n = 1.2, and m = 0.17, and temperature distribution trends between canals with different water contents were basically similar. Water will accumulate at the bottom as the liquid water content increases at the canal boundary.

The decomposition of maize residues has a vital influence on soil aggregate buildup–breakdown (turnover) processes, but the following remain unclear: i) which size aggregates of Mollisols are primarily affected by the decomposition of maize residues and ii) the turnover proportions of these aggregates. In the study, rare earth oxides (REOs) were used to trace and quantify the buildup–breakdown paths of soil aggregate fractions during the whole incubation time. The two treatments were designed as follows: i) REO-labelled soil with maize residues addition (maize residue treatment) and ii) REO-labelled soil without maize residues addition (control, CK). The soil respiration, aggregate size distribution, REO concentrations, SOC and pH were measured during 84 days of incubation. The results showed that on days 0–14, the incorporation of maize residues significantly increased soil respiration and promoted the formation of 2–5 mm and 0.25–2 mm aggregate fractions at the expense of

[Objective] The effects of erosion on the surface soil of erosion gullies in sloping cropland soils in a black soil area were studied in order to provide theoretical support for the rational utilization of soil resources and regional soil and water conservation work in black soil areas. [Methods] The study was conducted on erosion gullies with different degrees of development in Yanshou County, Harbin City, Heilongjiang Provicne. Particle size distribution and water storage properties of soil on the slope, at the bottom of the gullies and at the head of the gullies were quantitatively described. [Results] ① Soil bulk density, maximum water-storage capacity, maximum water-retention capacity, total storage capacity, dead storage capacity, and maximum effective storage capacity at different depths were significantly different (pthe 30-minute infiltration rate>the stable infiltration rate. As soil depth increased, the infiltration characteristics of the three indicators decreased between gully A2 and A4 for different erosion degree gullies. In the 20—40 cm layer, the infiltration characteristics of gully A1 to A4 followed the order of CK (control group)> gully A1> gully A4> gully A2> gully A3. Infiltration initially declined and then increased. With the development of the erosion gully, soil infiltration decreased and then increased. ③ The development of erosion gullies was the main reason for the change in clay content. The percentage of clay in the 0—40 cm layer tended to increase first and then decrease. The percentage of sand in the 0—20 cm layer tended to decrease first and then increase, showing a trend toward increased coarseness. ④ The soil degradation index followed the order of gully A1> gully A2> gully A3> gully A4 in the 0—20 cm layer. Soil degradation increased with the development of erosion gullies. Soil degradation was most severe in gully A4. [Conclusion] The differences in soil profiles was the main reason for the differences in physical properties of black soil. Soil water-holding capacity in the 0—20 cm layer was weakened and soil degradation became severe as erosion gullies developed.

ABSTRACTFour dimeric diketopiperazine stereoisomers (1–4) including two new ones (1–2) had been isolated from the culture broth of one marine-derived fungus Aspergillus sp. Z3, which was found in the gut of a marine isopod Ligia exotica. The planner structures and absolute configurations of the new compounds were determined by combination of NMR, HRESIMS, electronic circular dichroism calculation, Marfey’s method as well as single-crystal X-ray diffraction. Their cytotoxicity against the prostate cancer PC3 cell line was assayed by the MTT method.

We aimed to examine the association of milk intake with sleep disorders and their specific indicators. The current study included 768 adults aged 28–95 from Wenling, China. Milk intake was assessed using a food frequency questionnaire with ten food items, while sleep disorders were measured using the Pittsburgh Sleep Quality Index (PSQI), with higher scores indicating poorer sleep. The participants were divided into two groups according to the average intake of milk per week: rare intake (≤62.5 mL/week) and regular intake (>62.5 mL/week). Primary measurements were multivariate-adjusted odds ratios (ORs) with 95% confidence intervals (CIs) for the prevalence of sleep disorders concerning regular milk intake compared with rare intake. In secondary analyses, linear regression analyses were performed to assess the effects of milk intake on sleep disorders and their specific dimensions. Regular intake of milk did not have a significant association with sleep disorders compared with rare intake (adjusted OR: 0.72, 95%; CI: 0.51, 1.03), but this association was found to be pronounced with sleep disturbances (OR: 0.49, 95%; CI: 0.28, 0.87). Increased intake of milk was significantly associated with the lower scores of PSQI for sleep quality (β: −0.045, 95%; CI: −0.083, −0.007) and sleep disturbances (β: −0.059, 95%; CI: −0.090, −0.029), respectively. When stratified by age and gender, the benefits of milk intake for sleep disorders and sleep disturbances were more significant in older adults (≥65) and men than in younger persons and women. In summary, regular milk intake benefits sleep quality, which may contribute to nutritional psychiatric support for prevention against sleep disorders.

In order to identify the effects of the slope and precipitation intensity on the soil runoff depth and runoff rate, different tillage patterns (slope-ridge direction, horizontal slope-ridge direction, no-ridge farming) and different slopes (3° and 5°) were set up, and five typical rainfalls from June to September 2021 were selected, to dynamically monitor the soil-erosion dynamics of the test plots under different rainfall intensities. The results show that cross-slope-ridge cropping has a retention effect on runoff, which effectively inhibits the ineffective loss of rainfall confluence. Among these results, the variation range in the soil runoff depth under cross-slope-ridge treatment conditions was 0.11~0.94 mm, while that under the slope-ridge treatment and no-ridge treatment conditions was increased to 1.44~12.49 mm and 3.45~14.96 mm, respectively. It found that the loss of soil nutrients was significantly higher in the slope-ridge direction and in the no-ridge farming condition than in the horizontal slope-ridge direction. It is worth noting that, as the slope of the cultivated land increases, the erosive capacity of the precipitation runoff for the soil phosphorus increases, while the carrying capacity of the soil nitrogen decreases, and the correlation analysis results confirm that the corresponding relationship between the free diffusion capacity of the soil ammonium nitrogen and soil erosion is weaker than that between the nitrate nitrogen and soil erosion. The effects of single factors, such as the slope, ridge direction, and precipitation intensity of the cultivated land, have a significant impact on the soil water- and fertilizer-loss process, while the influence effect of the multi-factor coupling process on soil erosion is weakened. It was confirmed that the erosion process of rainfall runoff on soil nitrogen and phosphorus loss in slope cultivated land is the result of multi-factor action, and the artificial modification of the tillage mode can effectively regulate the effect of farmland water and fertilizer loss.

Sulfamethoxazole (SMX), is a ubiquitous antibiotic in the aquatic environment and received concerns on its health hazards, especially its sub-lethal effects on non-target organisms which were remained largely unknown. In the present study, in order to investigate SMX induced tissue damages and reveal underlying mechanisms, marine mussels, Mytilus galloprovincialis were challenged to SMX series (0.5, 50 and 500 μg/L) for six-days followed by six-day-recovery. Comprehensive histopathological alteration (including qualitative, semi-quantitative and quantitative indices), together with transcriptional and (post-) translational responses of key factors (p38, NFκB and p53) in the p38-MAPK signaling pathway were analyzed in gills and digestive glands. Tissue-specific responses were clearly investigated with gills showing more prompt responses and digestive glands showing higher tolerance to SMX. The histopathology showed that SMX triggered inflammatory damages in both tissues and quantitative analysis revealed more significant responses, suggesting its potential as a valuable health indicator. SMX activated expressions of p38, NFκB and p53 at transcriptional and (post-) translational levels, especially after exposed to low level SMX, evidenced by p38 coupled with NFκB/p53 regulation on immunity defense in mussels. Less induction of targeted molecules under severe SMX exposure indicated such signaling transduction may not be efficient enough and can result in inflammatory damages. Taken together, this study expanded the understanding of aquatic SMX induced health risk in marine mussels and the underlying regulation mechanism through p38 signaling transduction.

Abstract Background Impulsivity is more commonly reported in subjects with mental disorders compared to healthy subjects, suggesting a potential application of impulsivity in predicting impulsivity-related mental disorders. However, no biomarker of impulsivity available so far. This study explored the association between cerebrospinal fluid (CSF) fibroblast growth factor 21 (FGF21), a key hormonal mediator of the stress response, and impulsivity in healthy subjects. Methods A total of 126 healthy persons subjected to surgery of anterior cruciate ligament were recruited in the present study. The impulsiveness of the subjects was evaluated by the Chinese version of the Barratt Impulsiveness Scale (BIS)-11 before surgery. CSF and blood samples of the subjects were collected before spinal anesthesia for surgery. The levels of FGF21, serotonin and dopamine in CSF and the level of FGF21 in blood of the subjects were measured by ELISA using commercial kits. Results Negative correlations were found between BIS-11 total score and either FGF21, serotonin or dopamine in CSF. However, BIS-11 total score was not correlated with FGF21 in blood. In addition, FGF21 was positively correlated with serotonin and dopamine in CSF, respectively. Multivariable linear regression models indicated that the decrease of FGF21 level associating with the decrease of serotonin and dopamine level in CSF contributed to the higher impulsivity. Furthermore, receiver operating characteristic curve (ROC) analysis indicated an important role of CSF FGF21 predicting high impulsivity. Conclusions FGF21, serotonin and dopamine in CSF associate with impulsivity in opposite directions. The decrease of CSF FGF21 is related to higher impulsivity, and indicate that CSF FGF21 may predict impulsivity in healthy subjects.

Alcohol dependence (AD) is characterized by compulsive alcohol consumption, which involves behavioral impairments such as aggression. Members of fibroblast growth factor (FGF) 19 superfamily, including FGF19, FGF21, and FGF23, are major endocrine mediators that play an important role in alcohol metabolism and alcohol related disorders. The objective of the present study is to explore the possible associations among the interaction of single nucleotide polymorphisms (SNPs) of the FGF 19 superfamily, AD occurrence, and aggression in patients with AD. A total of 956 subjects were enrolled in this study, including 482 AD patients and 474 healthy controls (HCs). Michigan alcoholism screening test (MAST) was used to measure the level of AD, a Chinese version of the Buss–Perry Aggression Questionnaire was used to evaluate the aggressive behavior of subjects, and MassARRAY@ system was used to genotype rs948992 of FGF19, rs11665841 and rs11665896 of FGF21, rs7955866 and rs11063118 of FGF23. The results showed that AD patients presented a significantly higher level of aggression compared to HCs, and MAST scores were significantly positively associated Buss–Perry aggression scores (r = 0.402, p < 0.001) in AD patients. The interaction of FGF19 rs948992 TC × FGF21 rs11665896 GG presented the high-risk genotype combination predicting the high level of AD. In addition, the interaction of FGF19 rs948992 TC × FGF21 rs11665896 TG × FGF23 rs11063118 TT presented the high-risk genotype combination predicting the high level of aggression in AD patients. Our results added evidence linking the combination of rs948992 TC × rs11665896 TG × rs11063118 TT to aggressive behavior in AD patients and pointed out the potential usefulness of the SNPs of FGF19 superfamily as a predictor for the aggression in AD patients.

Marine natural products (MNPs) are an important source of biologically active metabolites, particularly for therapeutic agent development after terrestrial plants and nonmarine microorganisms. Sequencing technologies have revealed that the number of biosynthetic gene clusters (BGCs) in marine microorganisms and the marine environment is much higher than expected. Unfortunately, the majority of them are silent or only weakly expressed under traditional laboratory culture conditions. Furthermore, the large proportion of marine microorganisms are either uncultivable or cannot be genetically manipulated. Efficient heterologous expression systems can activate cryptic BGCs and increase target compound yield, allowing researchers to explore more unknown MNPs. When developing heterologous expression of MNPs, it is critical to consider heterologous host selection as well as genetic manipulations for BGCs. In this review, we summarize current progress on the heterologous expression of MNPs as a reference for future research.

Previous studies have proved that root distribution along gully headwalls greatly alters soil properties and further affects the soil erodibility of gully heads. However, it is not clear whether the gully headcut migration is affected by root distribution and soil properties. Five representative gullies developed in different land uses were selected to clarify the variations of root distribution and soil properties and their effects on headcut migration in the rainy season (May to October 2021) in the Mollisols region of northeast China. Results showed that the 68.4%–93.3% of root mass density and 65.6–88.5% of root length density were concentrated in 0–30 cm soil layer of gully heads, and the roots of 85%. The gullies developed in farmlands had relatively higher soil compactness, shear strength and aggregate stability, but lower organic matter (OMC), disintegration capacity and soil permeability than those developed in woodlands, unpaved roads in farmland and stable gully-beds. Changes in soil properties of gully heads were closely related to root density. The linear, areal, and volumetric migration rate of gully heads varied greatly and were 1.07–35.11 m yr−1, 28.95–562.46 m2 yr−1 and 56.82–6626.37 m3 yr−1, respectively, with the average of 9.07 m yr−1, 156.92 m2 yr−1 and 1503.02 m3 yr−1, respectively. The change in headcut migration rate was significantly affected by root density, soil properties and drainage area, of which soil texture, OMC, soil aggregate structure, and the drainage area were the critical factors influencing headcut migration in the Mollisols region of northeast China.

Objective(s):Higher cellular reactive oxygen species (ROS) levels is important in reducing cellular energy charge (EC) by increasing the levels of key metabolic protein, and nitrosative modifications, and have been shown to damage the cardiac tissue of diabetic mice. However, the relation between energy production and heart function is unclear. Materials and Methods:Streptozotocin (STZ, 150 mg/kg body weight) was injected intraperitoneally once to mice that had been fasted overnight for induction of diabetes. After diabetic induction, mice received citrate (5 µg/kg) through intraperitoneal injection every other day for 5 weeks. The caspase-3, plasminogen activator inhibitor 1 (PAI1), protein kinase B (PKB), commonly known as AKT and phosphorylated-AKT (p-AKT) proteins were examined to elucidate inflammation and apoptosis in the heart. For histological analysis, heart samples were fixed with 10% formalin and stained with hematoxylin-eosin (HE) and Sirius red to assess pathological changes and fibrosis. The expression levels[AGA1] of marker proteins, tyrosine nitration, activity of ATP synthase and succinyl-CoA:3-ketoacid coenzyme A transferase-1 (SCOT), and EC were measured. Results:Intraperitoneal injection of citrate significantly reduced caspase-3 and PAI-1 protein levels and increased p-AKT level on the 5th week; EC in the heart was found to be increased as well. Further, the expression level, activity, and tyrosine nitration of ATP synthase and SCOT were not affected after induction of diabetes. Conclusion: Results indicate that application of citrate, a tricarboxylic acid (TCA) cycle intermediate, might alleviate cardiac dysfunction by reducing cardiac inflammation, apoptosis, and increasing cardiac EC.

Marine-derived fungi have been reported to have great potential to produce structurally unique metabolites. Our investigation on secondary metabolites from marine-derived fungi resulted in the isolation of seven new polyketides (phomopsiketones D−G (1−4) and letendronols A−C (5−7)) as well as one known xylarinol (8) in the cultural broth of Letendraea sp. Their structures and absolute configurations were elucidated using a set of spectroscopic and chemical methods, including HRESIMS, NMR, single-crystal X-ray diffraction, ECD calculation, and a modified version of Mosher’s method. Compound 2 showed weak inhibition against nitric oxide production in lipopolysaccaride-activated macrophages with an IC50 value of 86 μM.

Two novel aspochalasins, 20-β-methylthio-aspochalsin Q (named as aspochalasin V), (1) and aspochalasin W (2), were isolated from culture broth of Aspergillus sp., which was found in the gut of a marine isopod Ligia oceanica. The structures were determined on the basis of NMR and mass spectral data analysis. This is the first report about methylthio-substituted aspochalasin derivatives. Cytotoxicity against the prostate cancer PC3 cell line and HCT116 cell line was assayed using the MTT method. Apochalasin V showed moderate activity at IC50 values of 30.4 and 39.2 μM, respectively.

Two novel aspochalasins, tricochalasin A (1) and aspochalasin A2 (2), along with three known compounds (3–5) have been isolated from the different culture broth of Aspergillus sp., which was found in the gut of a marine isopod Ligia oceanica. Compound 1 contains a rare 5/6/6 tricyclic ring fused with the aspochalasin skeleton. The structures were determined on the basis of electrospray ionisation mass spectroscopy (ESIMS), nuclear magnetic resonance (NMR) spectral data, and the absolute configurations were further confirmed by modified Mosher’s method. Cytotoxicity against the prostate cancer PC3 cell line were assayed by the MTT method. Compound 3 showed strong activity while the remaining compounds showed weak activity.

We have recently isolated four new melophlins P (1), Q (2), R (3), and S (4) together with seven known melophlins A (5), D (6), E (7), G (8), H (9), I (10), and O (11) from two marine sponges of the genus Melophlus collected in Palau. In this study, the influence of these compounds on the colony formation of Chinese hamster V79 cells and the production of IL-8 in PMA-stimulated HL-60 cells were examined. These 11 compounds did not show any effect on IL-8 production. The EC50 values of compounds 2, 3, 4, 5, 7, 9, 10, and 11 against V79 cells were 44.0, 13.3, 16.7, 27.2, 19.8, 8.5, 23.1, and 9.6 μM, respectively. The linear-chain-type compounds (1, 6, and 8) were not active against V79 cells at 50 μM. Although the growth inhibitory activity of these melophlins was not remarkable, some structure-activity relationships of these compounds against V79 and murine leukemia L1210 cells were observed.

Abstract: Bioassay-guided isolation from the ethanol extract of a marine sponge Theonella sp. collected in Palau yielded bistheonellide A, which strongly inhibited the colony formation of Chinese hamster V79 cells (EC50 = 6.8 nM). Bistheonellide A is an actinpolymerization inhibitor and was suggested to control cytokine production. Therefore, we attempted to detect an effect of this compound on IL-8 production in PMA-stimulated HL- 60 cells. Interestingly, bistheonellide A did not modulate the production of IL-8 under cytotoxic concentrations as determined by LDH analysis. Although the correlation between the inhibition of microtubule assembly and the stimulation of IL-8 production has been observed for several compounds, the polymerization of actin was not related to an IL-8 production in the case of bistheonellide A. It will be suggested that the actin polymerization is not involved in the IL-8 production system.