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In shrimp, several glutathione peroxidase (GPX) genes have been cloned and functionally studied. Increasing evidence suggests the genes’ involvement in white spot syndrome virus (WSSV)- or Vibrio alginolyticus-infection resistance. In the present study, a novel GXP gene (LvGPX3) was cloned in Litopenaeus vannamei. Promoter of LvGPX3 was activated by NF-E2-related factor 2. Further study showed that LvGPX3 expression was evidently accelerated by oxidative stress or WSSV or V. alginolyticus infection. Consistently, downregulated expression of LvGPX3 increased the cumulative mortality of WSSV- or V. alginolyticus-infected shrimp. Similar results occurred in shrimp suffering from oxidative stress. Moreover, LvGPX3 was important for enhancing Antimicrobial peptide (AMP) gene expression in S2 cells with lipopolysaccharide treatment. Further, knockdown of LvGPX3 expression significantly suppressed expression of AMPs, such as Penaeidins 2a, Penaeidins 3a and anti-lipopolysaccharide factor 1 in shrimp. AMPs have been proven to be engaged in shrimp WSSV- or V. alginolyticus-infection resistance; it was inferred that LvGPX3 might enhance shrimp immune response under immune challenges, such as increasing expression of AMPs. The regulation mechanism remains to be further studied. more...

In shrimp, several glutathione peroxidase (GPX) genes have been cloned and functionally studied. Increasing evidence suggests the genes’ involvement in white spot syndrome virus (WSSV)- or Vibrio alginolyticus-infection resistance. In the present study, a novel GXP gene (LvGPX3) was cloned in Litopenaeus vannamei. Promoter of LvGPX3 was activated by NF-E2-related factor 2. Further study showed that LvGPX3 expression was evidently accelerated by oxidative stress or WSSV or V. alginolyticus infection. Consistently, downregulated expression of LvGPX3 increased the cumulative mortality of WSSV- or V. alginolyticus-infected shrimp. Similar results occurred in shrimp suffering from oxidative stress. Moreover, LvGPX3 was important for enhancing Antimicrobial peptide (AMP) gene expression in S2 cells with lipopolysaccharide treatment. Further, knockdown of LvGPX3 expression significantly suppressed expression of AMPs, such as Penaeidins 2a, Penaeidins 3a and anti-lipopolysaccharide factor 1 in shrimp. AMPs have been proven to be engaged in shrimp WSSV- or V. alginolyticus-infection resistance; it was inferred that LvGPX3 might enhance shrimp immune response under immune challenges, such as increasing expression of AMPs. The regulation mechanism remains to be further studied. more...

In this work, we perform a study of nucleon resonance production in the γp→pηϕ reaction within an effective Lagrangian approach. In our model, we consider the excitation of the N*(1535), N*(1650), N*(1710), and N*(1720) in the intermediate state and the background term. We find that this reaction is dominated by the excitation of the N*(1535) in the near threshold region. Especially, we study the possible role of the scalar meson exchange in this reaction. It is found that the f0(980) exchange may give a significant contribution and the parity asymmetry can be used to identify its role in this reaction. more...

The interaction between a Mode I crack and an inclusion in an infinite medium is examined with consideration of coupled mechanical and thermal loads under plane strain condition. A closed-form formula for the change of the stress intensity factor is obtained based on the transformation toughening theory and the Eshelby inclusion method. The mismatched thermal expansion strains between the inclusion and the matrix are conducted as eigen strains. Then finite element method is implemented to validate the accuracy for the stress intensity factor variation from the developed formula. Finally, the effects of temperature-dependent elastic properties on the inclusion–crack interaction are estimated. It is also found that the shielding or amplifying effect on the crack growth is the dependence on the mismatched expansion coefficient if only the temperature increase or decrease is considered. more...

The free electron laser (FEL), as a next-generation light source, is an attractive tool in scientific frontier research because of its advantages of full coherence, ultra-short pulse duration, and controllable polarization. Owing to the demand of real-time bunch diagnosis during FEL experiments, precise nondestructive measurements of the polarization and X-ray energy spectrum using one instrument are preferred. In this paper, such an instrument based on the electron time-of-flight technique is proposed. By considering the complexity and nonlinearity, a numerical model in the framework of Geant4 has been developed for optimization. Taking the Shanghai Soft X-ray FEL user facility as an example, its measurement performances' dependencies on the critical parameters were studied systematically, and, finally, an optimal design was obtained, achieving resolutions of 0.5% for the polarization degree and 0.3 eV for the X-ray energy spectrum., 16 pages, 13 figures, Design of a Nondestructive Two-in-One Instrument for Measuring the Polarization and Energy Spectrum at an X-ray FEL Facility. Accepted by JINST more...