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Soil flooding is a serious abiotic stress that can repress plant growth and yield. The intensity of flooding stress is continuously increasing owing to rapid climate change and changes in rain intensity and frequency. Biochar (BC) emerges as an important soil amendment to mitigate adverse impacts of abiotic stress. The role of BC against different stresses is well reported, however, its role under flooding stress is not determined yet. Thus, this experiment was conducted to determine the role of BC on the performance of brassica crops under flooding stress. The experiment was comprised of well-watered (WW) and flooding stress (FS) conditions and biochar application: control, 1% biochar, and 2.5% biochar. The results indicated that flooding stress stunted the plant growth and impaired the photosynthetic pigments, leaf water status, osmolytes yield and yield traits, and oil concentration and increased the electrolyte leakage (EL), hydrogen peroxide (H2O2) and malondialdehyde (MDA) concentration. The application of BC at the rate of 2.5% mitigated the adverse impacts of salinity and upgraded the antioxidant activities (~50-120%), reduced the oxidative stress markets (~70-300%), and increased the leaf water status, photosynthetic pigments, osmolyte accumulation, nutrient uptake, yield traits, and oil contents. In conclusion, BC application can improve the productivity and oil yield of brassica under drought stress by improving plant physiological and biochemical functioning. However, more in depth studies are direly needed to explore the mechanism of BC to induce flood tolerance.

Deep vertical rotary tillage (DVRT) is an innovative soil tillage technology that has been widely adopted in China and shown significant potential in enhancing soil quality, optimizing water use efficiency, and increasing crop yields across diverse ecological and agronomic conditions. DVRT utilizes a vertical spiral drill bit for deep plowing, which preserves soil structure, reduces soil compaction, and improves water retention, making it particularly effective in regions facing climatic challenges such as drought. This review synthesizes the effects of DVRT on soil’s physical and chemical properties, crop root systems, photosynthesis, and water use efficiency, demonstrating its advantages in promoting robust root development and improving nutrient utilization. Although the technology has been applied successfully across various crops and regions, its nationwide adoption remains limited. This paper emphasizes the need for further research to refine the theoretical framework of DVRT and develop tailored strategies for different local conditions. Additionally, integrating DVRT with other agronomic practices and advancing machinery design, supported by policy measures, is essential for maximizing its benefits. In conclusion, DVRT presents a promising approach for sustainable agriculture in China, contributing to improved soil quality, increased crop yields, and enhanced food security.

In recent years, the TikTok platform has made a significant impact in the social media market worldwide, now holding a large market share in the short video service market popular with young people. Despite all this success, there is little research on how Internet users become loyal to TikTok. Hence, this study proposes a framework based on the use and gratification theory to evaluate the antecedents of adolescents’ continuous use motivations and behavior, and how they influence teenagers’ continuous use of TikTok. The survey population for this study was reached both online and offline. A total of 362 adolescent Chinese users of the TikTok platform were surveyed. The results show that self-expression, entertainment, information seeking, communication, and passing time were derived as adolescents’ motivations to use TikTok. Among these motivations, entertainment, communication, and passing time positively affect user satisfaction with TikTok. Furthermore, user satisfaction and loyalty positively affect the intention to continue using TikTok. Finally, this study advances understanding of adolescents’ use motivation of TikTok and provides new reference material for the continuous innovation of the short video format. AcknowledgmentThis study appreciated the partial financial support from Research Project of Anhui University of Science and Technology in 2023 (Project topic: Research on the construction and improvement strategy of mobile shopping experience evaluation system from the perspective of experience economy; Grant number: 2023yjrc30) and Key Research Projects of Humanities and Social Sciences in Colleges and Universities in Anhui Province (Project topic: Research on the Management Mechanism and Practical Path of Anhui Free Trade Zone; Grant number: SK2020A0199).

Background Dysfunction of vascular endothelial cells (ECs) plays a central role in the pathogenesis of cardiovascular complications in diabetes. SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 (SMARCA5) is a key regulator of chromatin structure and DNA repair, but its role in ECs remains surprisingly unexplored. The current study was designed to elucidate the regulated expression and function of SMARCA5 in diabetic ECs. Methods SMARCA5 expression was evaluated in ECs from diabetic mouse and human circulating CD34+ cells using quantitative reverse transcription polymerase chain reaction and Western blot. Effects of SMARCA5 manipulation on ECs function were evaluated using cell migration, in vitro tube formation and in vivo wound healing assays. Interaction among oxidative stress, SMARCA5 and transcriptional reprogramming was elucidated using luciferase reporter assay, electrophoretic mobility shift assay and chromatin immunoprecipitation. Results Endothelial SMARCA5 expression was significantly decreased in diabetic rodents and humans. Hyperglycemia-suppressed SMARCA5 impaired EC migration and tube formation in vitro, and blunted vasculogenesis in vivo. Contrarily, overexpression of SMARCA5 in situ by a SMARCA5 adenovirus-incorporated hydrogel effectively promoted the rate of wound healing in a dorsal skin punch injury model of diabetic mice. Mechanistically, hyperglycemia-elicited oxidative stress suppressed SMARCA5 transactivation in a signal transducer and activator of transcription 3 (STAT3)-dependent manner. Moreover, SMARCA5 maintained transcriptional homeostasis of several pro-angiogenic factors through both direct and indirect chromatin-remodeling mechanisms. In contrast, depletion of SMARCA5 disrupted transcriptional homeostasis to render ECs unresponsive to established angiogenic factors, which ultimately resulted in endothelial dysfunction in diabetes. Conclusion Suppression of endothelial SMARCA5 contributes to, at least in part, multiple aspects of endothelial dysfunction, which may thereby exacerbate cardiovascular complications in diabetes.

Soil acidity is a serious problem in agricultural lands as it directly affects the soil, crop production, and human health. Soil acidification in agricultural lands occurs due to the release of protons (H+) from the transforming reactions of various carbon, nitrogen, and sulfur-containing compounds. The use of biochar (BC) has emerged as an excellent tool to manage soil acidity owing to its alkaline nature and its appreciable ability to improve the soil’s physical, chemical, and biological properties. The application of BC to acidic soils improves soil pH, soil organic matter (SOM), cation exchange capacity (CEC), nutrient uptake, microbial activity and diversity, and enzyme activities which mitigate the adverse impacts of acidity on plants. Further, BC application also reduce the concentration of H+ and Al3+ ions and other toxic metals which mitigate the soil acidity and supports plant growth. Similarly, soil salinity (SS) is also a serious concern across the globe and it has a direct impact on global production and food security. Due to its appreciable liming potential BC is also an important amendment to mitigate the adverse impacts of SS. The addition of BC to saline soils improves nutrient homeostasis, nutrient uptake, SOM, CEC, soil microbial activity, enzymatic activity, and water uptake and reduces the accumulation of toxic ions sodium (Na+ and chloride (Cl-). All these BC-mediated changes support plant growth by improving antioxidant activity, photosynthesis efficiency, stomata working, and decrease oxidative damage in plants. Thus, in the present review, we discussed the various mechanisms through which BC improves the soil properties and microbial and enzymatic activities to counter acidity and salinity problems. The present review will increase the existing knowledge about the role of BC to mitigate soil acidity and salinity problems. This will also provide new suggestions to readers on how this knowledge can be used to ameliorate acidic and saline soils.

Climate-change-induced variations in temperature and rainfall patterns are a serious threat across the globe. Flooding is the foremost challenge to agricultural productivity, and it is believed to become more intense under a changing climate. Flooding is a serious form of stress that significantly reduces crop yields, and future climatic anomalies are predicted to make the problem even worse in many areas of the world. To cope with the prevailing flooding stress, plants have developed different morphological and anatomical adaptations in their roots, aerenchyma cells, and leaves. Therefore, researchers are paying more attention to identifying developed and adopted molecular-based plant mechanisms with the objective of obtaining flooding-resistant cultivars. In this review, we discuss the various physiological, anatomical, and morphological adaptations (aerenchyma cells, ROL barriers (redial O2 loss), and adventitious roots) and the phytohormonal regulation in plants under flooding stress. This review comprises ongoing innovations and strategies to mitigate flooding stress, and it also provides new insights into how this knowledge can be used to improve productivity in the scenario of a rapidly changing climate and increasing flood intensity.

Microcystis aeruginosa is the competitively dominant algal species in eutrophic waters and poses a serious threat to the aquatic ecological environment. To investigate the effects of eucalyptus tannins (TFL) and black water in eucalyptus plantations on M. aeruginosa, this study exposed M. aeruginosa to different concentrations (0 (control), 20, 50, 80, 110, and 140 mg L–1) of tannic acid (TA; hydrolyzed tannins, HT; reagent tannin), epigallocatechin gallate (EGCG; condensed tannins, CT; reagent tannin), eucalyptus tannins (TFL, complex tannin) and mixed TFL + Fe3+ solution (tannin: Fe3+ molar ratio = 1:10). The cell density, chlorophyll-a (Chl-a) content, superoxide dismutase (SOD) activity, malondialdehyde (MDA) and soluble protein (SP) contents of algae under tannin stress were determined, and the algal cell density treated with under the combination of TFL and Fe3+ was determined. The results showed a reduction in the Chl-a content of algal cells, which inhibited photosynthesis; leading to membrane lipid peroxidation; and the complexation of soluble proteins resulting in blocked protein synthesis were the main mechanisms by which tannins inhibited the growth of M. aeruginosa. TFL achieved the same inhibition of algal cells as the tannin reagent at the same concentration. At 4 d, TFL at 80 mg L–1 and above could achieve more than 54.87 % algal density inhibition. The inhibition rate of 80 mg L–1 and above TFL + Fe3+ on algal density was more than 75 %, indicating that TFL + Fe3+ had a stronger inhibitory effect on algal density. The results may facilitate the resource utilization of eucalyptus harvesting residues, explorations of the potential application of eucalyptus tannins in the control of M. aeruginosa, and provide new ideas for ecological algal inhibition in eucalyptus plantations.

Tea is an economical and most widely used beverage across the globe owing to its unique fragrance and flavor. Plant microbe interaction has emerged as an important topic which got the attention of scientists to improve plant performance. Tea microbes remained a prominent research topic for scientists over the years as tea microbes helps in nutrient cycling and stress management which in turn improve the tea growth, yield and quality. The roots of tea plants are colonized by various microbes including arbuscular mycorrhizal fungi (AMF), bacterial communities, and endophytes increase root growth, development and nutrient uptake which in turn improve tea growth, yield and quality. These microbes also increase the concentration of nutrients, amino acids, soluble proteins, flavonoids, catechuic acid, glucose, fructose, sucrose contents caffeine, and polyphenols concentration in tea plants. Besides this, these microbes also protect the tea plants from harmful pest and diseases which in turn leads to an appreciable improvement in plant growth and development. The most important goal of any farming system is to establish a system with production of maximum food while minimizing impacts on the environment. The present review article highlights the role of various microbes in improving the growth, yield and quality of tea plants. In addition, we also discussed the research gaps to improve our understanding about the role of tea microbes in improving tea growth, yield, pest and diseases resistance. We believe that this review will provide a better insight into the existing knowledge of tea microbes in improving tea growth and yield.

In recent years, extreme environmental cues such as abiotic stresses, including frequent droughts with irregular precipitation, salinity, metal contamination, and temperature fluctuations, have been escalating the damage to plants’ optimal productivity worldwide. Therefore, yield maintenance under extreme events needs improvement in multiple mechanisms that can minimize the influence of abiotic stresses. Polyamines (PAs) are pivotally necessary for a defensive purpose under adverse abiotic conditions, but their molecular interplay in this remains speculative. The PAs’ accretion is one of the most notable metabolic responses of plants under stress challenges. Recent studies reported the beneficial roles of PAs in plant development, including metabolic and physiological processes, unveiling their potential for inducing tolerance against adverse conditions. This review presents an overview of research about the most illustrious and remarkable achievements in strengthening plant tolerance to drought, salt, and temperature stresses by the exogenous application of PAs. The knowledge of underlying processes associated with stress tolerance and PA signaling pathways was also summarized, focusing on up-to-date evidence regarding the metabolic and physiological role of PAs with exogenous applications that protect plants under unfavorable climatic conditions. Conclusively, the literature proposes that PAs impart an imperative role in abiotic stress tolerance in plants. This implies potentially important feedback on PAs and plants’ stress tolerance under unfavorable cues.

Salinity stress (SS) is a serious abiotic stress and a major constraint to agricultural productivity across the globe. High SS negatively affects plant growth and yield by altering soil physio-chemical properties and plant physiological, biochemical, and molecular processes. The application of micronutrients is considered an important practice to mitigate the adverse effects of SS. Zinc (Zn) is an important nutrient that plays an imperative role in plant growth, and it could also help alleviate the effects of salt stress. Zn application improves seed germination, seedling growth, water uptake, plant water relations, nutrient uptake, and nutrient homeostasis, therefore improving plant performance and saline conditions. Zn application also protects the photosynthetic apparatus from salinity-induced oxidative stress and improves stomata movement, chlorophyll synthesis, carbon fixation, and osmolytes and hormone accumulation. Moreover, Zn application also increases the synthesis of secondary metabolites and the expression of stress responsive genes and stimulates antioxidant activities to counter the toxic effects of salt stress. Therefore, to better understand the role of Zn in plants under SS, we have discussed the various mechanisms by which Zn induces salinity tolerance in plants. We have also identified diverse research gaps that must be filled in future research programs. The present review article will fill the knowledge gaps on the role of Zn in mitigating salinity stress. This review will also help readers to learn more about the role of Zn and will provide new suggestions on how this knowledge can be used to develop salt tolerance in plants by using Zn.

Drought stress is significant abiotic stress that limits crop growth and productivity across the globe. The intensity of drought stress continuously rises due to rapid climate change. Drought-induced alterations in physiological and bio-chemical processes by generating membrane dis-stability, oxidative stress, nutritional imbalance and leading to substantial reduction in growth and productivity. Plants accumulate various osmolytes that protect themselves from abiotic stresses' harmful effects. Trehalose (Tre) is a non-reducing sugar found in multiple microbes ranging from bacteria to yeast and in plants and it possesses an excellent ability to improve drought tolerance. Trehalose appreciably enhanced the plant growth, and counter the drought induced damages by maintaining cellular membranes, plant water relations, stomatal regulation, photosynthetic activities, nutrient uptake, osmolyte accumulation, activating stress proteins and detoxifying the reactive oxygen species (ROS) by strengthening the anti-oxidant system. Therefore, it is essential to understand the mechanism of exogenous and endogenous Tre in mitigating the drought-induced damages and to identify the potential research questions that must be answered in the future. Therefore, to better appraise the potential benefits of Tre in drought tolerance in this review, we discussed the diverse physiological and molecular mechanisms regulated by Tre under drought stress. We have a complete and updated picture on this topic to orientate future research directions on this topic.

Tea is a popular beverage all around the world. Tea composition, quality monitoring, and tea identification have all been the subject of extensive research due to concerns about the nutritional value and safety of tea intake. In the last 2 decades, research into tea employing electrochemical biosensing technologies has received a lot of interest. Despite the fact that electrochemical biosensing is not yet the most widely utilized approach for tea analysis, it has emerged as a promising technology due to its high sensitivity, speed, and low cost. Through bibliometric analysis, we give a systematic survey of the literature on electrochemical analysis of tea from 1994 to 2021 in this study. Electrochemical analysis in the study of tea can be split into three distinct stages, according to the bibliometric analysis. After chromatographic separation of materials, electrochemical techniques were initially used only as a detection tool. Many key components of tea, including as tea polyphenols, gallic acid, caffeic acid, and others, have electrochemical activity, and their electrochemical behavior is being investigated. High-performance electrochemical sensors have steadily become a hot research issue as materials science, particularly nanomaterials, and has progressed. This review not only highlights these processes, but also analyzes and contrasts the relevant literature. This evaluation also provides future views in this area based on the bibliometric findings.

With the rapid development of electric vehicles, the safety accidents caused by the damage and failure of lithium-ion batteries under mechanical load are increasing gradually, which increases the significance of collision safety in lithium-ion batteries. The failure threshold of the cell in a free state is different from that of the cells in the module. Therefore, the safety characteristics of cells and modules under vertical dynamic impact conditions were studied in this paper. Lithium iron phosphate (LiFePO4) batteries and assembled 2-in-10 series modules with a 100% state of charge (SOC) were tested. Analyses included the voltage, temperature, and mechanical behavior of test samples under different impact loads, extrusion positions, and indenter shapes. The results showed that the damage behavior of a battery was closely related to the contact shape, contact area, and contact position. A smaller contact area led to greater deformation; moreover, the contact area being closer to the edge position meant greater deformation and weaker load-carrying capacity. The load-carrying capacity of the cell in a free state was weaker than that of the module, but the failure threshold of the cell in a free state was higher than that of the module. It can be concluded that the failure threshold of the cell cannot reflect the failure threshold of the module.

The concentration of greenhouse gases (GHGs) in the atmosphere has been increasing since the beginning of the industrial revolution. Nitrous oxide (N2O) is one of the mightiest GHGs, and agriculture is one of the main sources of N2O emissions. In this paper, we reviewed the mechanisms triggering N2O emissions and the role of agricultural practices in their mitigation. The amount of N2O produced from the soil through the combined processes of nitrification and denitrification is profoundly influenced by temperature, moisture, carbon, nitrogen and oxygen contents. These factors can be manipulated to a significant extent through field management practices, influencing N2O emission. The relationships between N2O occurrence and factors regulating it are an important premise for devising mitigation strategies. Here, we evaluated various options in the literature and found that N2O emissions can be effectively reduced by intervening on time and through the method of N supply (30–40%, with peaks up to 80%), tillage and irrigation practices (both in non-univocal way), use of amendments, such as biochar and lime (up to 80%), use of slow-release fertilizers and/or nitrification inhibitors (up to 50%), plant treatment with arbuscular mycorrhizal fungi (up to 75%), appropriate crop rotations and schemes (up to 50%), and integrated nutrient management (in a non-univocal way). In conclusion, acting on N supply (fertilizer type, dose, time, method, etc.) is the most straightforward way to achieve significant N2O reductions without compromising crop yields. However, tuning the rest of crop management (tillage, irrigation, rotation, etc.) to principles of good agricultural practices is also advisable, as it can fetch significant N2O abatement vs. the risk of unexpected rise, which can be incurred by unwary management.

Biochar application is considered an effective approach to mitigating nitrous oxide (N2O) emissions from agricultural soils. However, the mechanisms of biochar to mitigate N2O emissions from acidic red soils are still unclear. Therefore, the present study aims to underpin mechanisms associated with rice residue-based biochar in mitigating N2O emissions from acid soils. Soil treated with different rates of biochar control, from 1%, 2%, and 3%, and different soil properties, including soil pH, microbial biomass carbon (MBC), NH4+-N, NO3−-N, genes abundance (nosZ, nirK, AOA, and AOB), and enzymatic activities ((nitrate reductase (NR) and urease (UR)) were studied. The application of 3% biochar increased the soil pH (5.21–6.48), MBC (565–685 mg/kg), NO3−-N contents (24.23–44.5 mg/kg), genes abundance (nosZ, nirK, AOA, and AOB) and UR activity. The highest N2O emission (43.60 μg kg−1) was recorded and compared with the application of 1% (26.3 μg kg−1), 2% (18.33 μg kg−1), and 3% biochar (8.13 μg kg−1). Applying 3% biochar effectively reduced the N2O emission due to increased soil pH, MBC, NO3−-N contents, genes abundance (nosZ, nirK, AOA, and AOB), and weakened NH4+-N and NR activities. Therefore, increasing soil pH, genes abundance, and weakened nitrification following the addition of rice residue-based biochar can effectively reduce the N2O emissions from acidic red soils.

Transient elastography quantifies the propagation of a mechanically generated shear wave within a soft tissue, which can be used to characterize the elasticity and viscosity parameters of the tissue. The aim of our study was to combine numerical simulation and clinical assessment to define a viscoelastic index of liver tissue to improve the quality of early diagnosis of liver fibrosis. This is clinically relevant, as early fibrosis is reversible. We developed an idealized two-dimensional axisymmetric finite element model of the liver to evaluate the effects of different viscoelastic values on the propagation characteristics of the shear wave. The diagnostic value of the identified viscoelastic index was verified against the clinical data of 99 patients who had undergone biopsy and routine blood tests for staging of liver disease resulting from chronic hepatitis B infection. Liver stiffness measurement (LSM) and the shear wave attenuation fitting coefficient (AFC) were calculated from the ultrasound data obtained by performing transient elastography. Receiver operating curve analysis was used to evaluate the reliability and diagnostic accuracy of LSM and AFC. Compared to LSM, the AFC provided a higher diagnostic accuracy to differentiate early stages of liver fibrosis, namely F1 and F2 stages, with an overall specificity of 81.48%, sensitivity of 83.33% and diagnostic accuracy of 81.82%. AFC was influenced by the level of LSM, ALT. However, there are no correlation between AFC and Age, BMI, TBIL or DBIL. Quantification of the viscoelasticity of liver tissue provides reliable measurement to identify and differentiate early stages of liver fibrosis.

In this paper, the total flavonoid yield was evaluated, Holboellia latifolia Wall. Peel was used as the experimental material, and the ultrasonic-assisted extraction method was used to study the extraction process of total flavonoids in Holboellia latifolia Wall. Peel, and the precipitation rule during the brewing process Study and establish its dynamic model. The results showed that the best extraction process of Holboellia latifolia Wall. Total flavonoids: material-liquid ratio 1:35 (g I mL), ethanol concentration 75%, ultrasonic time 40 min, ultrasonic temperature 55°C; Holboellia latifolia Wall. When the brewing temperature of the peel is 60-90°C, the content of total flavonoids in the tea soup increases with increasing temperature; the total flavonoid concentration tends to be stable after brewing for 40 min; the leaching of total flavonoids follows a second-order kinetic reaction, temperature and leaching The rate constant k has a good linear relationship, and the apparent activation energy of total flavonoids is 247.69J I mol. [ABSTRACT FROM AUTHOR]

With n-hexane as extractant, the optimal process of ultrasonic-assisted extraction of grapefruit seed oil was optimized by response surface methodology. The fatty acid composition and physical and chemical properties of the obtained grapefruit seed oil were analyzed. The results showed that the best extraction process of grapefruit seed oil was liquidsolid ratio (mL/g) of 12, ultrasonic time of 31 min, ultrasonic power of 191 W, and ultrasonic temperature of 60 °C. Under this condition, grape seed oil extraction rate was 35.67. %. Four kinds of fatty acids were detected by GC-MS, which were palmitic acid (12.74%), linoleic acid (47.73%), oleic acid (32.37%), stearic acid (7.16%), unsaturated fatty acids. The content reaches 80.1%; the grapefruit seed oil is a pale yellow, clear and transparent oily liquid with the characteristic smell and taste of grapefruit seeds. The relative density (d2020) and refractive index (nd20) of grapefruit seed oil were 0.9025 and 1.4745, moisture and volatile matter 0.02%, insoluble impurities 0.03%, iodine value (I2) 95.256 g /100 g, saponification value (KOH) 189.56 mg / g, unsaponifiable 2.46 g / kg, acid value (KOH) and peroxide value were 1.08 mg / g and 2.935 mmol / kg, respectively, smoke point 192°C. All physical and chemical indicators can reach the Chinese edible vegetable oil standard. [ABSTRACT FROM AUTHOR]

The traditional Chinese medicine, Gegen, is the dried roots of leguminous plant Pueraria lobate (Willd) Ohwi, or Pueraria thomsonii Benth. As one of the first batch of plants approved by the Chinese Ministry of Health that could serve as Chinese medicine homologous food, the utilization of wild Gegen resource mainly falls in its roots and flowers, while its leaves are simply discarded as wastes. We want to provide scientific theory basis for comprehensive utilization of Gegen LPC (leaf protein concentration). We carried out analysis for abundance and composition of amino acids in Gegen leaf protein concentration (LPC), via the method of fuzzy recognition and ratio coefficient of amino acid (RCAA), employing whole egg protein as control as recommended by FAO of the United Nations, with the amino acid reference pattern by FAO/WHO as evaluation standard, and performed a comprehensive evaluation for Gegen LPC’s nutritional value as well as comparisons with 4 types of food proteins. We found that, there was a variety of amino acids in Gegen LPC, including at least 17 types of amino acids, 33.7% of which was consisted of essential amino acids, and the first restricting amino acid was valine (Val). The SRCAA of Gegen LPC reached 65.00 m, which indicated Gegen LPC as a protein source of great nutritional values. Gegen LPC is a protein source with great nutritional values. [ABSTRACT FROM AUTHOR]

Purpose: To isolate, purify and determine the antioxidant property of total flavonoids from the roots of Ardisia mamillata, so as to provide a theoretical basis for development of natural antioxidants. Results: The best conditions for separation and purification of total flavonoids from Ardisia mamillata roots were: use of ADS-7 resin, loading total flavonoid concentration of 0.8896 mg/mL, loading buffer flow rate of 1.5 mL/min, loading buffer pH of 4.48, elution ethanol concentration of 60 %, and flow rate of 2.5 mL/min. Under these conditions, the degree of purification of total flavonoids of Ardisia mamillata root was 76.43 ± 0.36 %, adsorption rate was 96.52 ± 0.19 %, while resolution rate was 99.31 ± 0.27 %. When the concentration of the purified total flavonoids was 4.0 mg/mL, its DPPH radical scavenging activity was stronger than that of the standard, butylated hydroxytoluene (BHT), but lower than that of vitamin C. Conclusion: ADS-7 resin is the best macroporous resin for the purification of total flavonoids from the radix of Ardisia mamillata Hance, under the optimized conditions. The purified total flavonoids of Ardisia mamillata root have stronger DPPH radical scavenging ability than the standard, BHT. [ABSTRACT FROM AUTHOR]

Purpose: To optimize the extraction of total alkaloids from the root bark of Catalpa ovata using response surface methodology, and to determine the insecticidal activity of the total alkaloids extracted. Methods: A combination of Box-Behnken design and response surface methodology (RSM) was used to optimize the acid water extraction of total alkaloids from the root bark of Catalpa ovata, with extraction rate of total alkaloids as index and the single factor experiment as basis, and the extraction time, material: liquid ratio and pH as 3 factors. The insecticidal activity of total alkaloids was determined against the three instar armyworm Mythimna separata (Lepidoptera: Noctuidae) and diamondback moth Plutella xylostella (Lepidoptera: Plutellidae). Results: The optimum extraction conditions for total alkaloids were: material: liquid ratio of 1:10; extraction time of 3 h, pH of 1.0, and simmering. Under these conditions, total alkaloid extraction was 8.62 %, which was very close to the experimental value. The results were accurate and reliable, with reference value. The insecticidal activity of the total alkaloids indicate that when the concentration of the total alkaloids was 10 mg/L, the fatality rate of Plutella xyllostella and oriental armyworm was over 89 %, but the insecticidal activity of the total alkaloids was lower than that of avermectin which was employed as a reference. Conclusion: The alkaloids from the root bark of Catalpa ovata are potential botanical insecticides. [ABSTRACT FROM AUTHOR]