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Nuclear receptor corepressor (NCoR1) interacts with various nuclear receptors and regulates the anabolism and catabolism of lipids. An imbalance in lipid/energy homeostasis is also an important factor in obesity and metabolic syndrome development. In this study, we found that the deletion of NCoR1 in intestinal epithelial cells (IECs) mainly activated the nuclear receptor PPARα and attenuated metabolic syndrome by stimulating thermogenesis. The increase in brown adipose tissue thermogenesis was mediated by gut-derived tricarboxylic acid cycle intermediate succinate, whose production was significantly enhanced by PPARα activation in the fed state. Additionally, NCoR1 deletion derepressed intestinal LXR, increased cholesterol excretion, and impaired duodenal lipid absorption by decreasing bile acid hydrophobicity, thereby reversing the possible negative effects of intestinal PPARα activation. Therefore, the simultaneous regulatory effect of intestinal NCoR1 on both lipid intake and energy expenditure strongly suggests that it is a promising target for developing metabolic syndrome treatment.

Abstract Background It is critical to age successfully for general health considering the aging of the global population. Successful aging is an important indicator to assess whether older individuals are experiencing a healthy life and high-quality aging. This study aimed to explore the longitudinal relationship between the frequency and number of participation in leisure activities and the maintenance of successful aging. Methods This longitudinal study included a total of 1,828 Chinese adults aged 60 years or older. The following six activities were gathered to reflect leisure activities: reading books or newspapers, gardening work, playing mahjong or cards, listening to radio or watching TV, raising pets or domestic animals, and engaging in social activities. We evaluated successful aging using a multidimensional model which includes the following five components: better self-reported health, good psychological status, high cognitive function, free of disability, and better physical activity level. Results This study showed a retention rate of 58.4% for successful aging during a 4-year follow-up. Participants who frequently engaged in reading books or newspapers, gardening work, playing mahjong or cards, and listening to radio or watching TV, had higher rates of future successful aging maintenance (OR: 1.50, 95% CI: 1.04–2.22 to 1.89, 95% CI: 1.26–2.812) than those who never participated in these activities. Compared to those with 0 leisure activity, participants with 3 (OR: 1.78, 95% CI: 1.14–2.77) and 4–6 (OR: 1.65, 95% CI: 1.01–2.71) leisure activities had a greater probability of maintaining successful aging. Conclusions These findings suggest that individuals who participated more frequently in more leisure activities had a higher chance of the maintenance of successful aging in older adults. Encouraging older people to frequently participate in a greater variety of leisure activities may be an effective way to maintain successful aging over time.

Abstract Background The health status of secondary school students has received widespread attention, and family plays an extremely important role in protecting and promoting their health. However, the relationship between family health and suboptimal health status (SHS) among secondary school students and its underlying mechanisms are unclear. This study aims to understand the prevalence of SHS among Chinese secondary school students and analyze the relationship between family health and SHS, and examine the mediating roles of perceived stress and problematic internet use. Methods The 2,094 secondary school students (52.6% boys, 47.4% girls, M age ± SD = 15.74 ± 1.78) of this study came from “2022 Psychology and Behavior Investigation of Chinese Residents, PBICR”. The chi-square test and t-test were used to analyze the prevalence of SHS in secondary school students with different characteristics. The SPSS PROCESS macro software was used to explore the relationship between family health and SHS and the mediating effects of perceived stress and problematic internet use. Results The prevalence of SHS among Chinese secondary school students was 40.7%, and older students, girls, non-only child, high school students, smokers, drinkers, and students living in the Northeast region of China had higher prevalence. Family health, SHS, perceived stress, and problematic internet use were significantly related (P

Abstract Background Apocrine carcinoma associated with Paget’s disease is a rare malignancy that typically manifests in elderly individuals, predominantly affecting the geriatric population. It commonly arises in regions rich in apocrine glands and often exhibits an insidious onset, potentially requiring several years to be diagnosed. Case presentation An 80-year-old male was simultaneously diagnosed with scrotal apocrine carcinoma (showing Paget changes) and early-stage gastric cancer. Whole-genome exome sequencing confirmed these as independent malignancies with minimal genetic overlap, indicating that they were two primary tumors. The patient initially underwent successful surgery but experienced recurrence and metastasis. Treatment with capecitabine and paclitaxel showed promising responses, highlighting similarities between breast and apocrine carcinomas. Challenges were noted in the use of genetic testing and drug susceptibility assessments for treatment guidance. Notably, HER-2 expression in metastatic lesions, a trait of apocrine carcinoma, has remained unexplored due to negative HER-2 FISH results and a lack of available targeted therapies in China. Conclusion Elderly patients often exhibit a lesser degree of aggressiveness toward treatment following a diagnosis of malignant tumors. It is imperative to carefully consider how to strike a balance between effective treatment and maintaining a satisfactory quality of life for these patients. This case underscores the complexity of treating coexisting rare cancers in older adults and emphasizes the need for personalized treatments and continued innovation in cancer therapy. The insights gained offer significant value in understanding and managing such rare cancer cases.

Abstract Background Postoperative delirium (POD) often occurs in oncology patients, further increasing the medical and financial burden. Robotic technology in lower abdominal tumors resection reduces surgical trauma but increases risks such as carbon dioxide (CO2) absorption. This study aimed to investigate the differences in their occurrence of POD at different end-tidal CO2 levels. Method This study was approved by the Ethics Committee of Affiliated Hospital of He Bei University (HDFY-LL-2022-169). The study was registered with the Chinese Clinical Trials Registry on URL: http://www.chictr.org.cn , Registry Number: ChiCTR2200056019 (Registry Date: 27/08/2022). In patients scheduled robotic lower abdominal tumor resection from September 1, 2022 to December 31, 2022, a comprehensive delirium assessment was performed three days postoperatively using the CAM scale with clinical review records. Intraoperative administration of different etCO2 was performed depending on the randomized grouping after intubation. Group L received lower level etCO2 management (31-40mmHg), and Group H maintained the higher level(41-50mmHg) during pneumoperitoneum. Data were analyzed using Pearson Chi-Square or Wilcoxon Rank Sum tests and multiple logistic regression. Preoperative mental status score, alcohol impairment score, nicotine dependence score, history of hypertension and diabetes, duration of surgery and worst pain score were included in the regression model along with basic patient information for covariate correction analysis. Results Among the 103 enrolled patients, 19 (18.4%) developed postoperative delirium. The incidence of delirium in different etCO2 groups was 21.6% in Group L and 15.4% in Group H, respectively, with no statistical differences. In adjusted multivariate analysis, age and during of surgery were statistically significant predictors of postoperative delirium. The breath-hold test was significantly lower postoperatively, but no statistical differences were found between two groups. Conclusion With robotic assistant, the incidence of postoperative delirium in patients undergoing lower abdominal tumor resection was not modified by different end-tidal carbon dioxide management, however, age and duration of surgery were positively associated risk factors.

In order to explore the influence of sugar treatment on the enrichment of γ-aminobutyric acid (GABA) in peanut sprouts, this paper selected the optimal application concentration of different types of exogenous sugars (mannose, sucrose and glucose). The effects of GABA on the germination of peanut seeds and the related substances of GABA anabolic metabolism were investigated and the possible mechanism of action was studied. The results showed that mannose, sucrose and glucose treatment could significantly (P

As an important subclass of metal–organic frameworks (MOFs), anion-pillars MOFs (APMOFs) have recently exhibited exceptional performances in separation and purification processes. The adjustment of pore sizes and environments of APMOFs can be finely tuned through judicious combination of organic ligands, anion pillars, and metal ions. Compared to widely investigated anion pillars, organic ligands are more crucial as they allow for a broader range of pore sizes and environments at the nanometer scale. Furthermore, different from the bidentate ligand-based APMOFs that typically form three-dimensional (3D) frameworks with pcu topology, APMOFs constructed using multidentate nitrogen­(N)-containing ligands (with a coordination number ≥ 3) offer opportunities to create APMOFs with diverse topologies. The larger dimensions and possible distortion of multidentate N-containing ligands prove advantageous for addressing multi-component hydrocarbon separations encompassing a broad spectrum of dynamic diameters. Therefore, this Review summarizes the structural characteristics of multidentate ligand-based APMOFs and their enhanced performances for gas separation and purification processes. Additionally, it discusses current challenges and prospects associated with constructing multidentate ligand-based APMOFs while providing prospects. This critical review will provide valuable insights and guides for designing and developing advanced multidentate ligand-based APMOF adsorbents.

Abstract Iso-butene (iso-C4H8) is an important raw material in chemical industry, whereas its efficient separation remains challenging due to similar molecular properties of C4 olefins. The ideal adsorbent should possess simultaneous high uptakes for 1,3-butadiene (C4H6) and n-butene (n-C4H8) counterparts, endowing high efficiency for iso-C4H8 separation in adsorption columns. Herein, a sulfate-pillared adsorbent, SOFOUR-DPDS-Ni (DPDS = 4,4′-dipyridyldisulfide), is reported for the efficient iso-C4H8 separation from binary and ternary C4 olefin mixtures. The rigidity in pore sizes and shapes of SOFOUR-DPDS-Ni exerts the molecular sieving of iso-C4H8, while exhibiting high C4H6 and n-C4H8 uptakes. The benchmark Henry’s selectivity for C4H6/iso-C4H8 (2321.8) and n-C4H8/iso-C4H8 (233.5) outperforms most reported adsorbents. Computational simulations reveal the strong interactions for C4H6 and n-C4H8. Furthermore, dynamic breakthrough experiments demonstrate the direct production of high-purity iso-C4H8 (>99.9%) from C4H6/iso-C4H8 (50/50, v/v), n-C4H8/iso-C4H8 (50/50, v/v), and C4H6/n-C4H8/iso-C4H8 (50/15/35, v/v/v) gas-mixtures.

Objective: The content of total flavonoids, antioxidant activity and anti-inflammatory capacity in different parts of Tetrastigma hemsleyanum Diels et Gilg which imitated wild planting were compared. Methods: Stems, leaves, root tubers and root whiskers of wild imitating-planted Tetrastigma hemsleyanum Diels et Gilg were first extracted by alcohol extraction technology, and the content of total flavonoids was compared among different parts. Then DPPH free radical scavenging rate, ABTS+ free radical scavenging rate, hydroxyl free radical scavenging rate and ferric ion reducing power were used to evaluate the antioxidant capacity of different parts of plants. Meanwhile, lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model was established to compare anti-inflammatory ability of different parts of wild planting Tetrastigma hemsleyanum Diels et Gilg by measuring the release of NO. Results: Under the optimal extraction conditions, the contents of total flavonoids in stem leaves, root tubers and root whiskers were 11.86±0.23, 8.48±0.10 and 7.52±0.02 mg·g−1, respectively. A total of 10 common peaks of Tetrastigma hemsleyanum Diels et Gilg were identified by HPLC fingerprint. Rutin (peak 6), quercetin (peak 8) and kaempferol (peak 9) were identified via comparisons with reference standards. The content of the three indexes was the highest in roots, followed by stems and leaves. The IC50 values of DPPH free radicals, ABTS+ free radicals and hydroxyl free radicals in different parts of the plant were detected, and the values of stem leaves were 0.2107, 0.2315 and 0.7625 mg/mL. Root tubers were 0.3134, 0.3758 and 0.8967 mg/mL. The roots were 0.2058, 0.2587 and 0.7856 mg/mL, respectively. The absorbance values of the reducing capacity of ferric ions in stem, tuber and root were 0.172, 0.153 and 0.184. Three parts of the wild imitating-wild plant were not toxic to RAW264.7 cells at the concentrations between 25~200 μg/mL and could effectively inhibit the release of NO induced by LPS that achieving a good anti-inflammatory effect. Conclusion: The results of this study provided a reference for the quality evaluation and a theoretical basis for waste recycling as well as subsequent whole resource development to the non-medicinal parts of Tetrastigma hemsleyanum Diels et Gilg from the wild.

Abstract The purpose of this study was to investigate the mechanism by which resveratrol (Res) inhibits apoptosis and promotes proliferation and differentiation of pre-osteoblastic MC3T3-E1 cells, laying the groundwork for the treatment of osteoporosis (OP). The TCMSP database was used to find the gene targets for Res. The GeneCards database acquire the gene targets for OP. After discovering the potential target genes, GO, KEGG, and Reactome enrichment analysis were conducted. Verifying the major proteins involved in apoptosis can bind to Res using molecular docking. CCK8 measured the proliferative activity of mouse pre-osteoblasts in every group following Res intervention. Alkaline phosphatase staining (ALP) and alizarin red staining to measure the ability of osteogenic differentiation. RT-qPCR to determine the expression levels of Runx2 and OPG genes for osteogenic differentiation ability of cells. Western blot to measure the degree of apoptosis-related protein activity in each group following Res intervention. The biological processes investigated for GO of Res therapeutic OP involved in cytokine-mediated signaling pathway, negative regulation of apoptotic process, Aging, extrinsic apoptotic signaling pathway in absence of ligand, according to potential therapeutic target enrichment study. Apoptosis, FoxO signaling pathway, and TNF signaling pathway are the primary KEGG signaling pathways. Recactome pathways are primarily engaged in Programmed Cell Death, Apoptosis, Intrinsic Apoptotic Pathway, and Caspase activation via extrinsic apoptotic signaling pathways. This research established a new approach for Res treatment of OP by demonstrating how Res controls the apoptosis-related proteins TNF, IL6, and CASP3 to suppress osteoblast death and increase osteoclastogenesis.

Traditional partial synchronous Byzantine fault tolerant (BFT) protocols are confronted with new challenges when applied to large-scale networks like IoT systems, which bring about rigorous demand for the liveness and consensus efficiency of BFT protocols in asynchronous network environments. HoneyBadgerBFT is the first practical asynchronous BFT protocol, which employs a reliable broadcast protocol (RBC) to broadcast transactions and an asynchronous binary agreement protocol (ABA) to determine whether transactions should be committed. DumboBFT is a follow-up proposal that requires fewer instances of ABA and achieves higher throughput than HoneyBadgerBFT, but it does not optimize the communication overhead of HoneyBadgerBFT.In this paper, we propose TortoiseBFT, a high-performance asynchronous BFT protocol with three stages. We can significantly reduce communication overhead by determining the order of transactions first and requesting missing transactions after. Our two-phase transaction recovery mechanism enables nodes to recover missing transactions by seeking help from 2f+1 nodes. To improve the overall throughput of the system, we lower the verification overhead of threshold signatures in HoneyBadgerBFT, DumboBFT, and DispersedLedger from On3 to On2. We develop a node reputation model that selects producers with stable network conditions, which helps to reduce the number of random lotteries. Experimental results show that TortoiseBFT improves system throughput, reduces transaction delays, and minimizes communication overhead compared to HoneyBadgerBFT, DumboBFT, and DispersedLedger.

Immune checkpoint inhibitors (ICIs) demonstrate unique advantages in the treatment of lung cancer and are widely used in the era of immunotherapy. However, ICIs can cause adverse reactions. Hematological toxicities induced by immunotherapy are relatively rare. Agranulocytosis, a rare hematologic adverse event associated with immune checkpoint inhibitors, has received limited attention in terms of treatment and patient demographics. Herein, we report the case of a 68-year-old male with non-small cell lung cancer(NSCLC) who received two cycles of programmed cell death-1 (PD-1) antibody sintilimab immunotherapy combined with albumin-bound paclitaxel and carboplatin chemotherapy and one cycle of sintilimab monotherapy. He was diagnosed with grade 4 neutropenia and sepsis (with symptoms of fever and chills) after the first two cycles of treatment. Teicoplanin was promptly initiated as antimicrobial therapy. The patient presented with sudden high fever and developed agranulocytosis on the day of the third cycle of treatment initiation, characterized by an absolute neutrophil count of 0.0×109/L. The patient was treated with granulocyte colony-stimulating factor but did not show improvement. He was then treated with corticosteroids, and absolute neutrophil counts gradually returned to normal levels. To the best of our knowledge, this is the first reported case of sintilimab-induced agranulocytosis in a patient with NSCLC. Sintilimab-induced severe neutropenia or agranulocytosis is a rare side effect that should be distinguished from chemotherapy-induced neutropenia and treated promptly with appropriate therapies; otherwise, the condition may worsen.

Background: Whether there is hypothalamic degeneration in Parkinson's disease (PD) and its association with clinical symptoms and pathophysiological changes remains controversial. Objectives: We aimed to quantify microstructural changes in hypothalamus using a novel deep learning-based tool in patients with PD and those with probable rapid-eye-movement sleep behavior disorder (pRBD). We further assessed whether these microstructural changes associated with clinical symptoms and free thyroxine (FT4) levels. Methods: This study included 186 PD, 67 pRBD, and 179 healthy controls. Multi-shell diffusion MRI were scanned and mean kurtosis (MK) in hypothalamic subunits were calculated. Participants were assessed using Unified Parkinson's Disease Rating Scale (UPDRS), RBD Questionnaire-Hong Kong (RBDQ-HK), Hamilton Depression Rating Scale (HAMD), and Activity of Daily Living (ADL) Scale. Additionally, a subgroup of PD (n = 31) underwent assessment of FT4. Results: PD showed significant decreases of MK in anterior-superior (a-sHyp), anterior-inferior (a-iHyp), superior tubular (supTub), and inferior tubular hypothalamus when compared with healthy controls. Similarly, pRBD exhibited decreases of MK in a-iHyp and supTub. In PD group, MK in above four subunits were significantly correlated with UPDRS-I, HAMD, and ADL. Moreover, MK in a-iHyp and a-sHyp were significantly correlated with FT4 level. In pRBD group, correlations were observed between MK in a-iHyp and UPDRS-I. Conclusions: Our study reveals that microstructural changes in the hypothalamus are already significant at the early neurodegenerative stage. These changes are associated with emotional alterations, daily activity levels, and thyroid hormone levels.

Chinese cabbage (Brassica campestris L. ssp. chinensis (L.) Makino) stands as a widely cultivated leafy vegetable in China, with its leaf morphology significantly influencing both quality and yield. Despite its agricultural importance, the precise mechanisms governing leaf wrinkling development remain elusive. This investigation focuses on ‘Wutacai’, a representative cultivar of the Tacai variety (Brassica campestris L. ssp. chinensis var. rosularis Tsen et Lee), renowned for its distinct leaf wrinkling characteristics. Within the genome of ‘Wutacai’, we identified a total of 18 YUCs, designated as BraWTC_YUCs, revealing their conservation within the Brassica genus, and their close homology to YUCs in Arabidopsis. Expression profiling unveiled that BraWTC_YUCs in Chinese Cabbage exhibited organ-specific and leaf position-dependent variation. Additionally, transcriptome sequencing data from the flat leaf cultivar ‘Suzhouqing’ and the wrinkled leaf cultivar ‘Wutacai’ revealed differentially expressed genes (DEGs) related to auxin during the early phases of leaf development, particularly the YUC gene. In summary, this study successfully identified the YUC gene family in ‘Wutacai’ and elucidated its potential function in leaf wrinkling trait, to provide valuable insights into the prospective molecular mechanisms that regulate leaf wrinkling in Chinese cabbage.

IntroductionGastric cancer, characterized by high incidence and substantial disease burden, has drawn continuous attention regarding its occurrence and prognosis. Genetics plays a crucial role in influencing the prognosis of gastric cancer, and single nucleotide polymorphisms are closely associated with the occurrence, development, and prognosis of this malignant tumor. Our study aims to conduct survival analysis on patients carrying different single nucleotide polymorphisms, exploring the relationship between miRNA single nucleotide polymorphisms and the prognosis of gastric cancer.MethodsGenetic data from 344 patients in Xianyou, Fujian, formed the basis of our study. We delineated the survival rate and median survival time, utilizing the log-rank test and COX regression analysis as statistical tools.ResultsUpon stratifying the data by sex or operation, it was discerned that the GG genotype at MSH2 rs17502941 independently posed a heightened risk for gastric cancer. Other stratification analyses suggested that the subsequent single nucleotide polymorphisms were correlated with patient prognosis: rs17502941, rs884225, rs1468063, rs7143252, and rs2271738.DiscussionThe outcomes of this study strongly suggest that miRNA polymorphisms significantly influence the survival time of gastric cancer patients and can serve as effective predictors for the prognosis of gastric cancer.

The effective separation of acetylene (C2H2) from carbon dioxide (CO2) and ethylene (C2H4) presents considerable challenges in the petrochemical industry. In this work, we report a novel sulfate-pillared (SO42–) ultra-microporous material, denoted as SOFOUR-DPDS-Ni (SOFOUR = SO42–, 4-DPDS = 4,4′-dipyridyldisulfide), for efficient C2H2 capture from both CO2 and C2H4. The sulfate pillars play a crucial role in inducing robust negative electrostatic potentials within the intralayer cavities and interlayer channels, thereby facilitating the selective recognition of C2H2. As a result, SOFOUR-DPDS-Ni demonstrates a remarkable C2H2 adsorption capacity of 1.60 mmol g–1 at 0.01 bar, an exceptional selectivity of 174 for the 50/50 C2H2/CO2 mixture, and a high selectivity of 65 for the 1/99 C2H2/C2H4 mixture. These impressive metrics position SOFOUR-DPDS-Ni as a promising adsorbent for benchmark C2H2 separations. Dynamic breakthrough experiments validate its outstanding performance in separating C2H2 from both the CO2 and C2H4 mixtures. Computational simulations reveal the strong interactions between C2H2 and sulfate pillars, shedding light on the underlying mechanisms driving the adsorption process.

Abstract Olefin/paraffin separations are among the most energy-intensive processes in the petrochemical industry, with ethylene being the most widely consumed chemical feedstock. Adsorptive separation utilizing molecular sieving adsorbents can optimize energy efficiency, whereas the size-exclusive mechanism alone cannot achieve multiple olefin/paraffin sieving in a single adsorbent. Herein, an unprecedented sieving adsorbent, BFFOUR-Cu-dpds (BFFOUR = BF4 -, dpds = 4,4’-bipyridinedisulfide), is reported for simultaneous sieving of C2-C4 olefins from their corresponding paraffins. The interlayer spaces can be selectively opened through stronger guest-host interactions induced by unsaturated C = C bonds in olefins, as opposed to saturated paraffins. In equimolar six-component breakthrough experiments (C2H4/C2H6/C3H6/C3H8/n-C4H8/n-C4H10), BFFOUR-Cu-dpds can simultaneously divide olefins from paraffins in the first column, while high-purity ethylene ( > 99.99%) can be directly obtained through the subsequent column using granular porous carbons. Moreover, gas-loaded single-crystal analysis, in-situ infrared spectroscopy measurements, and computational simulations demonstrate the accommodation patterns, interaction bonds, and energy pathways for olefin/paraffin separations.

Abstract Background The sperm flagellum is an evolutionarily conserved specialized organelle responsible for sperm motility and male fertility. Deleterious mutations in genes involved in the sperm flagellum assembly can often cause sperm motility defects and male infertility. The murine Dnali1 gene encodes a protein that is known to interact with the cytoplasmic dynein heavy chain 1. Results A Dnali1-mutated mouse model was generated by inducing a nonsense mutation in the Dnali1 gene. The Dnali1-mutated male mice presented impaired sperm motility and were completely infertile. Although no obviously abnormal sperm morphology was observed in Dnali1-mutated male mice, the ultrastructural structure of sperm flagellum was disrupted, displaying as an asymmetrical distribution of the longitudinal columns (LCs). Notably, infertile Dnali1-mutated male mice were able to obtain offspring via ICSI. Conclusions Our results uncover a role of DNALI1 in sperm motility and male fertility in mice, and demonstrate that ICSI overcomes Dnali1-associated male infertility, thus providing guidance for the diagnosis and genetic counseling of DNALI1-associated human infertility.

Abstract Organic nitrates are key components of atmospheric organic aerosols. Hydrolysis is one of their main transformation pathways, affecting atmospheric nitrogen cycle and the properties of organic aerosols. Studying hydrolysis using experiments is hindered by limited authentic chemical standards. To advance our understanding on the hydrolysis of organic nitrates, we apply quantum chemistry methods here to establish a structure-activity relationship of the mechanisms and kinetics by selecting eight organic nitrates as model compounds. The results indicate that an acid-catalyzed mechanism is dominant for the most considered organic nitrates at pH corresponding to ambient organic aerosol (pH < 5). More importantly, a hydrolysis pathway driven by the shift of hydrogen or methyl cation is unveiled. Based on the revealed mechanisms, quaternary C at the α-site, tertiary/quaternary C at the β-site, and –C=C at the β/γ-site of the −ONO2 group are determined to be the key structural factors for the fast hydrolysis kinetics. An important feature for the hydrolysis of organic nitrates with such structural factors is proceeding via a carbocation intermediate. The formed carbocation could further mediate the organic aerosol chemistry, affecting the composition and properties of organic aerosols. This study provides a basis to further develop predictive models for hydrolysis kinetics of organic nitrates.

Purpose: Knee adduction, flexion moment, and adduction angle are often used as surrogate parameters of knee medial force. To verify whether these parameters are suitable as surrogates under different walking states, we investigated the correlation between knee medial loading with the surrogates during walking and turning. Methods: Sixteen healthy subjects were recruited to complete straight walk (SW), step turn (ST), and crossover turn (CT). Knee joint moments were obtained using inverse dynamics, and knee medial force was computed using a previously validated musculoskeletal model, Freebody. Linear regression was used to predict the peak of knee medial force with the peaks of the surrogate parameters and walking speed. Results: There was no significant difference in walking speed among these three tasks. The peak knee adduction moment (pKAM) was a significant predictor of the peak knee medial force (pKMF) for SW, ST, and CT (p < 0.001), while the peak knee flexion moment (pKFM) was only a significant predictor of the pKMF for SW (p = 0.034). The statistical analysis showed that the pKMF increased, while the pKFM and the peak knee adduction angle (pKAA) decreased significantly during CT compared to those of SW and ST (p < 0.001). The correlation analysis indicated that the knee parameters during SW and ST were quite similar. Conclusions: This study investigated the relationship between knee medial force and some surrogate parameters during walking and turning. KAM was still the best surrogate parameter for SW, ST, and CT. It is necessary to consider the type of movement when comparing the surrogate predictors of knee medial force, as the prediction equations differ significantly among movement types.

Objective To analyze the difference of Ct value of HBsAg-/HBV DNA + in blood samples from different types of voluntary blood donors by double ELISA and HBV DNA (MP6) detection, and to investigate the correlation between Ct value and the frequency of repeated blood donation, the first nucleic acid reactivity and the interval time of previous blood donation, so as to provide reference for laboratory evaluation of the effectiveness of nucleic acid testing(NAT) strategy for repeated blood donors occult hepatitis B virus infection(OBI). Methods The Ct value and information of blood donors from February 2019 to January 2022 in our laboratory were collected. According to the cumulative number of blood donations, they were divided into two groups:first-time blood donor group (Group A) and repeated blood donor group (Group B). Group B was subdivided into Group C 1( twice of blood donation) and group C 2(three or more times of blood donation) according to the cumulative times of blood donation, and Group D 1(< 1 year), Group D 2(1-3 years), Group D 3(3 years or more) according to the first NAT reactivity and the time of previous blood donation, the difference of Ct value and resolution yeild of HBV DNA in each group was compared. The yeild of HBV DNA in two groups was compared by chi-square test, and the difference of Ct values were compared by Nonparametric test. Results From February 2019 to January 2022, a total of 270 283 blood donors were tested, including 135 695 in Group A and 134 588 in Group B. The yeild of HBV DNA in Group A was 0.150% (203/135 695), which was higher than that in Group B [0.083% (111/134 588)] (P

Abstract The study aimed to develop an active packaging, functionalized by spent coffee grounds extracts (SCGE) to preserve cake. The antioxidant extracts were obtained using three extraction methods: conventional extraction (CE), ultrasound‐assisted extraction (UAE), and microwave‐assisted extraction (MAE). The SCGE obtained with UAE and MAE produced the highest total phenolic contents and antioxidant activities, and were incorporated into bilayer films composed of polylactic acid, konjac glucomannan, and wheat gluten. The SCGE improved the film's antioxidant activity (8.40 to 90. 56%) and oxygen transmission rate (5.05 ± 0.04 to 2.22 ± 0.13). Active packaging with microwave extracts was more effective in preserving cake's lipids than the ultrasound extracts as measured by the peroxide value, thiobarbituric acid, and acid value during 21 days of storage. Overall, the study demonstrates the potential safe utilization of coffee waste in active packaging.

Abstract Objective To determine whether white matter hyperintensity (WMH) volumes in specific regions are associated with Parkinson's disease (PD) compared to non‐PD controls, and to assess their impact on motor signs through cross‐sectional and longitudinal analyses. Methods A total of 50 PD participants and 47 age‐ and gender‐matched controls were enrolled. All PD participants were followed up for at least 2 years. To detect regions of greater WMH in the PD, the WMH volume of each region was compared with the corresponding region in the control group. Linear regression and linear mixed effects models were respectively used for cross‐sectional and longitudinal analyses of the impact of increases in WMH volume on motor signs. Results The PD group had greater WMH volume in the occipital region compared with the control group. Cross‐sectional analyses only detected a significant correlation between occipital WMH volume and motor function in PD. Occipital WMH volume positively correlated with the severity of tremor, and gait and posture impairments, in the PD group. During the follow‐up period, the participants' motor signs progressed and the WMH volumes remained stable, no longitudinal association was detected between them. The baseline occipital WMH volume cannot predict the progression of signs after adjustment for baseline disease duration and the presence of vascular risk factors. Interpretation PD participants in this study were characterized by greater WMH at the occipital region, and greater occipital WMH volume had cross‐sectional associations with worse motor signs, while its longitudinal impact on motor signs progression was limited.

BackgroundsImproving quality of life (QOL) is one of the main aims of lung transplantation (LTx). There is a need to identify those who have poor quality of life early. However, research addressing inter individual quality of life variability among them is lacking. This study aims to identify group patterns in quality of life among lung transplant recipients and examine the predictors associated with quality of life subgroups.MethodsIn total, 173 lung transplant recipients were recruited from one hospital in Guangdong Province between September 2022 and August 2023. They were assessed using the Lung Transplant Quality of Life scale (LT-QOL), Mindful Attention Awareness Scale (MAAS), Life Orientation Test-Revised scale (LOT-R), and Positive and Negative Affect Scale (PANAS). Latent profile analysis was used to identify QOL subtypes, and logistic regression analysis was used to examine the associations between latent profiles and sociodemographic and psychosocial characteristics.ResultsTwo distinct QOL profiles were identified: “low HRQOL” profile [N = 53 (30.94%)] and “high HRQOL” profile [N = 120 (69.06%)]. Single lung transplant recipients, and patients who reported post-transplant infection, high levels of negative emotion or low levels of mindfulness and optimism were significantly correlated with the low QOL subgroup.ConclusionUsing the domains of the LT-QOL scale, two profiles were identified among the lung transplant recipients. Our findings highlighted that targeted intervention should be developed based on the characteristics of each latent class, and timely attention must be paid to patients who have undergone single lung transplantation, have had a hospital readmission due to infection, exhibit low levels of optimism, low levels of mindfulness or high negative emotions.

Objective To systematically evaluate the efficacy and safety of butylphthalide combined with donepezil versus butylphthalide monotherapy for the treatment of vascular dementia. Methods Randomized controlled trials were searched in electronic databases, including PubMed, Embase, the Cochrane Library, China National Knowledge Infrastructure, Chinese Science and Technology Periodical Database (VIP), Wan Fang, and China Biology Medicine from inception to 29 November 2022. Two reviewers independently screened the papers and extracted data from the included studies. The data were processed using RevMan5.4 statistical software. Results Nine randomized controlled trials (n = 1024) were included in this meta-analysis. Regarding the primary outcomes, compared with butylphthalide monotherapy, combined butylphthalide and donepezil treatment exhibited significantly greater total clinical efficacy (relative risk = 1.24, 95% confidence interval [1.17, 1.31]) and did not increase the adverse event rate (relative risk = 1.39, 95% confidence interval [0.91, 2.14]). Regarding the secondary outcomes, the meta-analysis results for the Mini-Mental State Examination, abilities of daily living, and Montreal Cognitive Assessment scores and the interleukin-6, tumor necrosis factor-α, and superoxide dismutase blood levels all supported combined butylphthalide and donepezil treatment. Conclusion Butylphthalide combined with donepezil may be a better treatment strategy than donepezil alone for the treatment of vascular dementia in clinical practice.

Abstract Background Intervertebral disc degeneration (IVDD) is a common chronic disease in orthopedics, and its molecular mechanisms are still not well explained. Aim This study's objective was to bioinformatics‐based discovery of IVDD biomarkers and immune‐inflammatory infiltrates. Materials and Methods The IVDD illness gene collection was gathered from GeneCards, DisGeNet, and gene expression profiles were chosen from the extensive Gene Expression Omnibus database (GSE124272, GSE150408, and GSE153761). The STRING database was used to create a network of protein–protein interactions, while the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases were used for functional enrichment analysis. Using hub genes, the immune cell infiltration between IVDD patient samples and control tissues was examined. Finally, quantitative polymerase chain reaction and Western blot experiments were used to verify the expression of hub genes. Results A total of 27 differentially expressed hub genes were identified by bioinformatics. According to GO and KEGG analyses, hub genes were prominent in immunological responses, chemokine‐mediated signaling pathways, and inflammatory responses, with the key signaling pathways engaged in cellular senescence, apoptosis, Th1 and Th2 cell differentiation, and Th17 cell differentiation. Immune cell infiltration research revealed that T cells, lymphocytes, B cells, and NK cells were decreased in IVDD patients while monocytes, neutrophils, and CD8 T cells were increased. The expression levels of the senescence hub genes SP1, VEGFA, IL‐6, and the apoptosis key gene CASP3 were considerably greater in the IVDD model group than in the control group, according to in vitro validation. Conclusion In conclusion, the cellular senescence signaling pathway, the apoptosis signaling pathway, and associated hub genes play significant roles in the development and progression of IVDD, this finding may help direct future research on the senescence signaling route in IVDD.

AimTo investigate the association between cognitive function and body composition in older adults.MethodsWe collected data on 2080 older adults (>60 years of age) from the National Health and Nutrition Examination Survey (NHANES) for the years 1999–2000 and 2001–2002. Candidate variables included: demographic data (sex, age, race, education level, marital status, poverty-to-income ratio), alcohol consumption, cardiovascular disease, diabetes, osteoporosis, total bone mineral density, and total fat mass. A logistic regression model was established to analyze the association between cognitive function and body composition in older adults. In addition, stratified logics regression analysis was performed by sex and age.ResultsBone mineral density significantly affects cognitive function in older adults (p

Background: Parkinson's disease (PD) patients exhibit an imbalance between neuronal activity and perfusion, referred to as abnormal neurovascular coupling (NVC). Nevertheless, the underlying molecular mechanism and how levodopa, the standard treatment in PD, regulates NVC is largely unknown. Material and methods: A total of 52 drug-naïve PD patients and 49 normal controls (NCs) were enrolled. NVC was characterized in vivo by relating cerebral blood flow (CBF) and amplitude of low-frequency fluctuations (ALFF). Motor assessments and MRI scanning were conducted on drug-naïve patients before and after levodopa therapy (OFF/ON state). Regional NVC differences between patients and NCs were identified, followed by an assessment of the associated receptors/transporters. The influence of levodopa on NVC, CBF, and ALFF within these abnormal regions was analyzed. Results: Compared to NCs, OFF-state patients showed NVC dysfunction in significantly lower NVC in left precentral, postcentral, superior parietal cortex, and precuneus, along with higher NVC in left anterior cingulate cortex, right olfactory cortex, thalamus, caudate, and putamen (P-value

Abstract Increasing evidence suggests that Parkinson’s disease (PD) exhibits disparate spatial and temporal patterns of progression. Here we used a machine-learning technique—Subtype and Stage Inference (SuStaIn) — to uncover PD subtypes with distinct trajectories of clinical and neurodegeneration events. We enrolled 228 PD patients and 119 healthy controls with comprehensive assessments of olfactory, autonomic, cognitive, sleep, and emotional function. The integrity of substantia nigra (SN), locus coeruleus (LC), amygdala, hippocampus, entorhinal cortex, and basal forebrain were assessed using diffusion and neuromelanin-sensitive MRI. SuStaIn model with above clinical and neuroimaging variables as input was conducted to identify PD subtypes. An independent dataset consisting of 153 PD patients and 67 healthy controls was utilized to validate our findings. We identified two distinct PD subtypes: subtype 1 with rapid eye movement sleep behavior disorder (RBD), autonomic dysfunction, and degeneration of the SN and LC as early manifestations, and cognitive impairment and limbic degeneration as advanced manifestations, while subtype 2 with hyposmia, cognitive impairment, and limbic degeneration as early manifestations, followed later by RBD and degeneration of the LC in advanced disease. Similar subtypes were shown in the validation dataset. Moreover, we found that subtype 1 had weaker levodopa response, more GBA mutations, and poorer prognosis than subtype 2. These findings provide new insights into the underlying disease biology and might be useful for personalized treatment for patients based on their subtype.

Abstract As one of the least understood aerosol processes, nucleation can be a dominant source of atmospheric aerosols. Sulfuric acid (SA)-amine binary nucleation with dimethylamine (DMA) has been recognized as a governing mechanism in the polluted continental boundary layer. Here we demonstrate the importance of trimethylamine (TMA) for nucleation in the complex atmosphere and propose a molecular-level SA-DMA-TMA ternary nucleation mechanism as an improvement upon the conventional binary mechanism. Using the proposed mechanism, we could connect the gaseous amines to the SA-amine cluster signals measured in the atmosphere of urban Beijing. Results show that TMA can accelerate the SA-DMA-based new particle formation in Beijing by 50–100%. Considering the global abundance of TMA and DMA, our findings imply comparable importance of TMA and DMA to nucleation in the polluted continental boundary layer, with probably higher contributions from TMA in polluted rural environments and future urban environments with controlled DMA emissions.

The incidence of knee injuries during firefighter training is high, but there is a lack of research on predictive factors and risk assessment for such injuries. Biomechanical assessments can provide a better understanding of how the body’s load changes during exercise, which may alter the risk of injury. Ten firefighters were recruited for our study. Each participant completed the FMS test and the single- and double-leg jump tasks. Motion information was collected and musculoskeletal models of the participants were constructed using OpenSim 4.4 to obtain the joint angle and joint moment. The peak GRF and CoM-CoP angle were also calculated. The findings showed a significantly larger PKFM (p = 0.0195), VAFM (p = 0.0039), and peak AP GRF (p = 0.0039) during the single-leg jump. The opposite performance was observed for KFA (p = 0.0098) and MPA (p = 0.0273). A stepwise multiple linear regression analysis was used to explore the relationship between these parameters and both the FMS score and the times of injuries. The risk of injury is higher in the single-leg jump compared to the double-leg jump. The biomechanical parameters of these two jumps can be used to assess sports injuries and to provide methods and references for injury risk monitoring during firefighter occupational training.

The effect of adding different proportions of transglutaminase (TG) on the structure and digestive characteristics of high-moisture pea protein extrudates prepared with a twin-screw extruder was investigated. The macrostructure and microstructure of the extrudates were characterized by measuring texture and texturization degree as well as using a scanning electron microscope (SEM). The structural properties and nutritional value were evaluated by measuring rheological properties, protein solubility and digestive properties. These results showed that addition of appropriate amounts of TG (0.25%–­1.00%) promoted the cross-linking between pea protein molecules, forming a dense network structure, increasing the digestibility of extrudates and promoting the release of free amino acids. However, addition of excess TG (2%) inhibited the fiber formation and digestive properties of extrudates. The extrudate with 1.00% of TG had higher texturization degree and nutritional value. This study demonstrated that high-moisture extrusion combined with addition of TG can improve the structure and digestive characteristics of plant protein-based meat analogs.

Abstract Background Different anesthetics may have opposite effects on the immune system, thus affecting the prognosis of tumor patients. Cell-mediated immunity forms the primary defense against the invasion of tumor cells, so manipulation of the immune system to produce an enhanced anti-tumor response could be utilized as an adjuvant oncological therapy. Sevoflurane has proinflammatory effects, while propofol, has anti-inflammatory and antioxidant effects. Therefore, we compared the overall survival (OS) and disease-free survival (DFS) of patients with esophageal cancer under total intravenous anesthesia and inhalation anesthesia. Methods This study collected the electronic medical records of patients undergoing esophagectomy from January 1, 2014 to December 31, 2016. According to the intraoperative anesthetics, the patients were divided into total intravenous anesthesia (TIVA) group or inhalational anesthesia (INHA) group. Stabilized inverse probability of treatment weighting (SIPTW) was used to minimize differences. Kaplan–Meier survival curve was established to evaluate the correlation between different anesthesia methods in overall survival and disease-free survival of patients undergoing esophageal cancer surgery. Results A total of 420 patients with elective esophageal cancer were collected, including 363 patients eligible for study (TIVA, n = 147, INHA, n = 216). After SIPTW there were no significant differences between two groups in overall survival and disease-free survival. However, the adjuvant therapy was statistically significant in improving OS, and the degree of differentiation was correlated with OS and DFS. Conclusions In conclusion, there were no significant difference in overall survival and disease-free survival between total intravenous anesthesia and inhalational anesthesia in patients undergoing esophageal cancer surgery.