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Abstract Coordination cages have been widely reported to bind a variety of guests, which are useful for chemical separation. Although the use of cages in the solid state benefits the recycling, the flexibility, dynamicity, and metal-ligand bond reversibility of solid-state cages are poor, preventing efficient guest encapsulation. Here we report a type of coordination cage-integrated solid materials that can be swelled into gel in water. The material is prepared through incorporation of an anionic FeII 4L6 cage as the counterion of a cationic poly(ionic liquid) (MOC@PIL). The immobilized cages within MOC@PILs have been found to greatly affect the swelling ability of MOC@PILs and thus the mechanical properties. Importantly, upon swelling, the uptake of water provides an ideal microenvironment within the gels for the immobilized cages to dynamically move and flex that leads to excellent solution-level guest binding performances. This concept has enabled the use of MOC@PILs as efficient adsorbents for the removal of pollutants from water and for the purification of toluene and cyclohexane. Importantly, MOC@PILs can be regenerated through a deswelling strategy along with the recycling of the extracted guests.

Methane, the second most prevalent greenhouse gas globally, is characterized by its high global warming potential and short atmospheric lifetime. It is primarily emitted from sectors such as coal mining, oil and gas production, agriculture, livestock rearing, and waste management. Active, prudent, and orderly control of methane emissions offers a multifaceted benefit: it mitigates global warming, enhances the economic value through the resource utilization of energy, provides environmental advantages by control of pollutants, and improves safety by reducing production accidents. Methane is also the principal greenhouse gas emitted in coal mine operations. Currently, monitoring and researching the distribution of methane emissions and their concentration within the three-dimensional space of coal mines have become a key focus in China’s methane emission control efforts, which is of significant importance for coal mining enterprises to align with national strategies. A study conducted on a high-gas mine in Shanxi and a low-gas mine in Shaanxi for methane emission monitoring and accounting utilized fixed monitoring, manual monitoring, drone monitoring, and post-mining activity monitoring to investigate the distribution of methane in high and low gas mines in China and to analyze their methane emissions. The results indicate that: ① The methane emission volumes for a certain high-gas mine in Shanxi and a low-gas mine in Shaanxi published by the U.S.-based Climate TRACE website for the year 2022 show a significant discrepancy from the results calculated using the ground measurement method, with the published annual methane emission volume for a certain low-gas mine being 10.92 times that of the ground measurement calculation. The top-down monitoring and accounting methods have been found to be insufficiently accurate and unable to provide reliable data support for carbon trading. ② In the monitoring and accounting of post-mining activities, the use of default values recommended by the IPCC results in methane emissions that are 3 to 5 times higher than those calculated using the ground measurement method, and there is a significant variation in the emissions calculated based on coal samples of different sizes and exposure durations. There is an urgent need for a unified and precise testing standard to ensure fair and accurate data for future carbon market transactions.

Abstract Polypeptide N-Acetylgalactosaminyltransferase (GALNTs) are critical enzymes that initiate mucin type-O glycosylation, and are closely associated with the occurrence and development of multiple cancers. However, the significance of GALNT2 in clear cell renal cell carcinoma (ccRCC) progression remains largely undetermined. Based on public multi-omics analysis, GALNT2 was strongly elevated in ccRCC versus adjoining nontumor tissues, and it displayed a relationship with poor overall survival (OS) of ccRCC patients. In addition, GALNT2 over-expression accelerated proliferation of renal cancer cell (RCC) lines. In contrast, GALNT2 knockdown using shRNAs suppressed cell proliferation, and this was rescued by LATS2 knockdown. Similarly, GALNT2 deficiency enhanced p-LATS2/LATS2 expression. LATS2 is activated by phosphorylation (p-LATS2) and, in turn, phosphorylate the downstream substrate protein YAP. Phosphorylated YAP (p-YAP) stimulated its degradation and cytoplasmic retention, as it was unable to translocate to the nucleus. This resulted in reduced cell proliferation. Subsequently, we explored the upstream miRNAs of GALNT2. Using dual luciferase reporter assay, we revealed that miR-139-5p interacted with the 3ʹ UTR of GALNT2. Low miR-139-5p expression was associated with worse ccRCC patient outcome. Based on our experiments, miR-139-5p overexpression inhibited RCC proliferation, and this phenotype was rescued by GALNT2 overexpression. Given these evidences, the miR-139-5p-GALNT2-LATS2 axis is critical for RCC proliferation, and it is an excellent candidate for a new therapeutic target in ccRCC.

Prospective doctoral students face a daunting challenge choosing between Doctor of Education (EdD) programs and Doctor of Philosophy (PhD) in Education due to programmatic ambiguity, inconsistency, and ill-defined career alignment (Carpenter, 1987; Perry, 2012; Shafer & Giblin, 2008). This qualitative study employed comparative analysis to explore the distinctions between 50 US EdD programs, including completion time, modality, credits, qualifying exam (QE) inclusion and requirements, and dissertation requirements. The theoretical framework used to investigate the root causes and potential outcomes of the EdD and PhD inconsistency included Foucault’s Power Theory (Aguirre Rojas, 2021) and Adam’s Equity Theory (Adams, 1963, 1965). Findings revealed significant differences between EdD programs and between EdD and PhD programmatic features. This data provides valuable insight for prospective students, informs EdD improvement, and urges consistency or standardization for clarity, integrity, and advancement in the field (Fisher et al., 2020; McMahon et al., 2020; Schafer & Giblin, 2008).

Indoor pollutants change over time and place. Exposure to hazardous organics is associated with adverse health effects. This work sampled gaseous organics by Tenax TA tubes in two indoor rooms, i.e., an office set as samples, and the room of chassis dynamometer (RCD) set as backgrounds. Compounds are analyzed by a thermal desorption comprehensive two-dimensional gas chromatography-quadrupole mass spectrometer (TD-GC × GC-qMS). Four new chemicals of emerging concern (CECs) are screened in 469 organics quantified. We proposed a three-step pipeline for CECs screening utilizing GC × GC including 1) non-target scanning of organics with convincing molecular structures and quantification results, 2) statistical analysis between samples and backgrounds to extract useful information, and 3) pixel-based property estimation to evaluate the contamination potential of addressed chemicals. New CECs spotted in this work are all intermediate volatility organic compounds (IVOCs), containing mintketone, isolongifolene, β-funebrene, and (5α)-androstane. Mintketone and sesquiterpenes may be derived from the use of volatile chemical products (VCPs), while (5α)-androstane is probably human-emitted. The occurrence and contamination potential of the addressed new CECs are reported for the first time. Non-target scanning and the measurement of IVOCs are of vital importance to get a full glimpse of indoor organics.

Abstract Background Current clinical guidelines recommend surgery only for cT1-2N0M0 small cell lung cancer (SCLC) patients. In light of recent studies, the role of surgery in the treatment of SCLC needs to be reconsidered. Methods We reviewed all SCLC patients who underwent surgery from November 2006 to April 2021. Clinicopathological characteristics were retrospectively collected from medical records. Survival analysis was performed by the Kaplan–Meier method. Independent prognostic factors were evaluated by Cox proportional hazard model. Results 196 SCLC patients undergoing surgical resection were enrolled. The 5-year overall survival for the entire cohort was 49.0% (95% CI: 40.1–58.5%). PN0 patients had significantly superior survival to pN1–2 patients (p

The inconsistent findings about learning outcomes in VR (virtual reality) learning necessitate further robustness of empirical data. This article addressed this gap by comparing the learning outcomes across VR, phone, and mobile learning on two dimensions: recall accuracy and recall speed, as well as learners' attitudes. Additionally, this paper attempted to investigate the differences between 5 and 10 min time duration. By inviting a total of 28 participants to the lab experiments on English vocabulary acquisition, this study yielded no statistically significant difference in vocabulary recall accuracy between VR, computer, and mobile learning. Nonetheless, the significant difference in vocabulary recall speed was generated. Besides, the time spent on vocabulary acquisition was significantly shorter in VR than in computer and mobile learning. Additionally, pairwise comparisons revealed that learners' perceptions and attitudes toward SUS resulted in significantly lower scores in VR than in computer- and mobile-based learning. Further, merely two of the nine items on the VR sickness scale demonstrated a significant raise in visual fatigue as well as significant decline in headache between 5 and 10 min learning duration. Future research should anticipate a larger sample size to ensure the validity of research findings and further construct a 3D modeling for the same language learning settings for non-college, primary, as well as secondary school students.

Plant litter decomposition figures importantly in the cycling of C and N pools in terrestrial ecosystems. We investigated how C and N fluxes changed during the decomposition of leguminous and non-leguminous leaf litters, and how these processes responded to different precipitation regimes. We used the dual-isotope tracing method to investigate differences in leaf and soil C and N, along with δ13C and δ15N, in the soil of the Loess Plateau in China. The δ15N and δ13C values were 3604‰ and 56‰ for Robinia pseudoacacia (Leguminosae) and 8115‰ and 452‰ for Populus tomentosa (Salicaceae) leaf litters. Through decomposition, δ13C decreased in all litters, and δ15N in the leguminous litter increased while it decreased in the non-leguminous litter. In the surface soil, the fraction of litter-derived N (14%) from the leguminous litter was significantly higher than that of the non-leguminous litter after 16 months. The C and N concentrations of both litters and soil always had a positive correlation during decomposition, and the responses of N to C changes in soil were reduced by the litter cover. Increased precipitation enhanced the litters’ C and N correlation. The 600 mm precipitation treatment most benefited litter C’s transformation to SOC, and drought conditions promoted the transformation of legume litter N to soil TN, but inhibited non-legume litter N. In the soil and both litters, C and N changes always had a positive correlation. After 16 months, the proportion of soil N from legumes was higher than that from non-legumes. Reduced precipitation could promote leguminous N in soil. Our results provide a scientific basis for accurately predicting the C and N cycles in terrestrial ecosystems.

Abstract A member of the newly discovered RNA family, circular RNA (circRNA) is considered as the intermediate product of by-product splicing or abnormal RNA splicing. With the development of RNA sequencing, circRNA has recently drawn research interest. CircRNA exhibits stability, species conservatism, and tissue cell specificity. It acts as a miRNA sponge in the circRNA-microRNA (miRNA-mRNA axis, which can regulate gene transcription and protein translation. Studies have confirmed that circRNA is ubiquitous in eukaryotic cells, which play an important role in the regulation of human gene expression and participate in the occurrence and development of various human diseases. CircRNA may be closely related to the occurrence and development of female reproductive system diseases. By analyzing the biological functions and mechanism of circRNA, we find that circRNA has certain development prospects as biomarkers of the female reproductive system diseases. The production and degradation of circRNA, biological functions, and their association with the occurrence of diseases of female reproductive system are reviewed in this article.

Acidic poly(ionic liquid)s (PILs) with swelling ability were synthesized by free radical copolymerization of N-vinylimidazolium ionic liquids, divinylbenzene (DVB) and sodium acrylate (NaAA), and further acidification by sulfuric acid. The swelling ability of acidic PILs was greatly affected by cross-linker content and chain length of 3-alkyl-substituents on imidazolium. Cross-linked network structures could be observed from the cryogenic scanning electron microscopy (cryo-SEM) images of the swollen acidic PILs in formic acid. Acidic PILs with network structures in swollen state exhibited excellent activities in the esterification of cyclohexene and formic acid, and the catalytic activities were in positive correlation with their swelling abilities. Acidic PIL with 3-octyl-substituent and 2.5 mol% DVB (PIL-C8-2.5DVB-HSO4) had the highest swelling ability in formic acid and exhibited comparable catalytic activities with homogeneous catalysts such as sulfuric acid and p-toluenesulfonic acid.

An isotope-dilution liquid chromatography tandem mass spectrometry method was established for the determination of vitamin B12 in milk and dairy products. The samples were spiked with stable isotope-labeled vitamin B12 and digested by pepsin and amylase. The various forms of cobalamin were transformed to cyanocobalamin by potassium cyanide after they were released from the enzymatically digested samples. Cyanocobalamin was extracted and purified by an immunoaffinity SPE cartridge and then measured in multiple reaction monitoring mode (MRM). The linear correlation coefficient (R2) of this method was greater than 0.999 in the range of 2–100 ng/mL. The detection limit and the quantification limit were 0.5 μg/kg and 1.0 μg/kg, respectively. The spiked recoveries ranged from 92.0% to 99.4% at the three spiked levels with the relative standard deviation (RSD) between 1.89% and 4.51%. The measured results of NIST SRM1849a and NIST SRM1869a by the current method are all within the reference value range. The Z value was 0.8 during participating in the FAPAS proficiency test using the developed method in 2021. The method is simple, rapid, accurate, and sensitive, and it is suitable for the determination of vitamin B12 in different types of milk and dairy products such as whey powder, whole milk powder, pure milk, fermented milk, infant formula, and prescription food for special medical purposes.

Abstract Polycystic ovary syndrome (PCOS) is a prevalent hormonal disorder of premenopausal women worldwide and is characterized by reproductive, endocrine, and metabolic abnormalities. The clinical manifestations of PCOS include oligomenorrhea or amenorrhea, hyperandrogenism, ovarian polycystic changes, and infertility. Women with PCOS are at an increased risk of suffering from type 2 diabetes; me\tabolic syndrome; cardiovascular events, such as hypertension, dyslipidemia; gynecological diseases, including infertility, endometrial dysplasia, endometrial cancer, and ovarian malignant tumors; pregnancy complications, such as premature birth, low birthweight, and eclampsia; and emotional and mental disorders in the future. Although numerous studies have focused on PCOS, the underlying pathophysiological mechanisms of this disease remain unclear. Mitochondria play a key role in energy production, and mitochondrial dysfunction at the cellular level can affect systemic metabolic balance. The recent wide acceptance of functional mitochondrial disorders as a correlated factor of numerous diseases has led to the presupposition that abnormal mitochondrial metabolic markers are associated with PCOS. Studies conducted in the past few years have confirmed that increased oxidative stress is associated with the progression and related complications of PCOS and have proven the relationship between other mitochondrial dysfunctions and PCOS. Thus, this review aims to summarize and discuss previous and recent findings concerning the relationship between mitochondrial dysfunction and PCOS.

The separation and simultaneous quantitation of diastereomers of DL-α-tocopherol, eight tocol forms, and retinols (trans and cis) have been conducted by reversed-phase liquid chromatography followed by solid-phase extraction. A chiral silica stationary phase modified with polysaccharide derivative on the monodisperse macroporous silica gel (Unichiral OD-5H column, 150 mm × 4.6 mm, 5 μm, NanoMicro Technology Co., Ltd.) was employed for eluting each target compound. Instead of conventional solvent extract, a green and eco-friendly solid-phase extraction column, packing with nonpolar polystyrene divinylbenzene, was optimized in terms of capacity and solvent used in steps. Validation of the method was examined and confirmed to be satisfactory, with excellent linearity regression (r > 0.9999), acceptable accuracy (74.66%∼112.92%), and precision (0.20%∼10.52%) results. Limit of detection ranged from 0.05 mg·kg−1 (retinols) to 0.4 mg·kg−1 (tocols). The method was checked by infant formula reference material SRM 1849a as well, which illustrated good agreement of mass fraction with certified value and enriched the important isomer data.

LAT1 is a member of the system L transporter family. The main role of the LAT1 is to transport specific amino acids through cell membranes to provide nutrients to cells and participate in several metabolic pathways. It also contributes to the transport of hormones and some drugs, which are essential for the development and treatment of some diseases. In recent years, many studies have shown that LAT1 is related to cancer, obesity, diabetes, and other diseases. However, the specific mechanism underlying the influence of LAT1 on such conditions remains unclear. Through the increasing number of studies on LAT1, we have obtained a preliminary understanding on the function of LAT1 in diseases. These studies also provide a theoretical basis for finding treatments for LAT1-related diseases, such as cancer. This review summarizes the function and mechanism of LAT1 in different diseases and the treatment of LAT1-related diseases. It also provides support for the development of novel and reliable disease treatments.

Abstract Background Polycystic ovary syndrome (PCOS) is a heterogeneous endocrine disorder in women of reproductive age and is commonly complicated by adverse endometrial outcomes. Long non-coding RNAs (lncRNAs) are a class of non-protein-coding transcripts that are more than 200 nucleotides in length. Accumulating evidence indicates that lncRNAs are involved in the development of various human diseases. Among these lncRNAs, lncRNA CD36–005 (CD36–005) is indicated to be associated with the pathogenesis of PCOS. However, the mechanisms of action of CD36–005 have not yet been elucidated. Methods This study determined the CD36–005 expression level in the uteri of PCOS rat model and its effect on the proliferation activity of rat primary endometrial stromal cells. RNA sequencing (RNA-seq) and bioinformatics analysis were performed to detect the mRNA expression profiles and the biological pathways in which these differentially expressed mRNAs involved, after CD36–005 overexpression in the primary endometrial stromal cells. The differential expression of Hmgn5, Nr5a2, Dll4, Entpd1, Fam50a, and Brms1 were further validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results CD36–005 is highly expressed in the uteri of PCOS rat model and promotes the proliferation of rat primary endometrial stromal cells. A total of fifty-five mRNAs differentially expressed were identified in CD36–005 overexpressed stromal cells. Further analyses identified that these differentially expressed mRNAs participate in many biological processes and are associated with various human diseases. The results of qRT-PCR validation were consistent with the RNA-seq data. Conclusions These data provide a list of potential target mRNA genes of CD36–005 in endometrial stromal cells and laid a foundation for further studies on the molecular function and mechanism of CD36–005 in the endometrium.

Purpose: The purpose of this quantitative study was to fill the knowledge gap and to investigate relationships between cultural background and various demographic factors influencing parental involvement behaviors that prompt them to engage in their children's academic activities. Design/methodology/approach: A quantitative research method was used to collect data to answer research questions and explore relationships between variables (Fraenkel et al., 2015). The researchers created the survey on Qualtrics and conducted a pilot study to improve the survey based on the recommendations of the pilot study's participants. Some items were reworded suggested by an expert committee to finalize the instrument. This survey mainly consisted of two sections to collect data on participants' demographics and cultural background. The participants used multiple-choice options to answer items pertaining to family demographics. They were asked to use a 5-point Likert scale: very often = 5, often = 4, sometimes = 3, rarely = 2 and never = 1 to respond to survey items regarding cultural background and parental involvement behaviors. Findings: The correlation coefficient showed a statistically significant relationship between parents' expectations, educational level and their involvement behaviors in children's academic achievement. However, parents' income and cultural background had no statistically significant relationships with parental involvement in their children's academic achievement. Research limitations/implications: The results of this study have potentially broad implications for educational leaders, policymakers, educators and parents to develop policies for diverse students to enhance their educational achievements. Originality/value: The researchers reviewed extensive literature and found the gap in regional studies particularly related to one of the fastest-growing, financially stable and highly educated ethnic groups in the country. The researchers developed a brand new instrument on Qualtrics and distributed a survey via online and direct administration to collect primary data from 200 participants.

In this paper, we report a 12-week longitudinal study aiming at exploring the students' reading outcome and cognitive load with individual-based print, mobile app of Rain Classroom and collaboration-based social media of WeChat. Administered to 186 postgraduate students in a research university were the weekly reading materials and comprehension tests, together with the self-designed CLCR scale after the reading sessions. A combination of one-way and repeated measures ANOVA along with multi-regression analysis showed that social media produced the best reading outcome; The same pattern occurred about enhanced reading outcome in the initial sessions followed by a decline at the end; Reading outcome in print other than in mobile and social media significantly increased from the first session to the ending session; Reading in mobile generated the highest level of extraneous, intrinsic, and germane load; Multi-regression analysis showed a negative relationship between intrinsic load and the prediction of collaborative reading across all the reading contexts. Further efforts may contribute to more complex patterns of reading outcome and cognitive load status by arranging individual- and collaboration-based contexts in different sequences for students with high and low level of cognitive load.

Previous studies have examined patterns of withdrawal from initial teacher education (ITE) programs and have found that pre-service teachers are more likely to withdraw if they are male or older than the typical pre-service teacher. This study presents case studies based on semi-structured interviews with older male pre-service teachers who withdrew from a large one-year post-Bachelor’s ITE program. To better understand the experiences of these pre-service teachers and the reasons they withdrew, we present each case as a unique narrative before examining the similarities and differences in their expectations and why they withdrew from the program. We discuss possible interpretations of and approaches to preventing withdrawal for stakeholders of ITE programs.

Dealing with local challenges and providing effective support for students as an international faculty member can be both challenging and rewarding. This phenomenological study delved into the intricate dynamics of cross-cultural encounters within higher education settings, focusing on the experiences of international faculty in supporting local students. Using semi-structured focus group meetings, the researchers explored how their lived experiences as full-time international faculty working in institutions of higher learning in the United States could be applied in providing support for students. This process required intricate navigation to acquire some level of cultural understanding of the local culture and traditions and build relationships with native faculty, staff, and students. The study was based on firsthand experiences of how the authors, international scholars from various parts of the world, navigated through these hurdles to become effective educators. The researchers constructed generalizations, noting that the institutions whose policies include embracing cultural diversity, fostering inclusivity, and providing targeted support are better positioned to enable their international faculty members to address local challenges and empower students to succeed effectively. [ABSTRACT FROM AUTHOR]

Prospective doctoral students face a daunting challenge choosing between Doctor of Education (EdD) programs and Doctor of Philosophy (PhD) in Education due to programmatic ambiguity, inconsistency, and illdefined career alignment (Carpenter, 1987; Perry, 2012; Shafer & Giblin, 2008). This qualitative study employed comparative analysis to explore the distinctions between 50 US EdD programs, including completion time, modality, credits, qualifying exam (QE) inclusion and requirements, and dissertation requirements. The theoretical framework used to investigate the root causes and potential outcomes of the EdD and PhD inconsistency included Foucault’s Power Theory (Aguirre Rojas, 2021) and Adam’s Equity Theory (Adams, 1963, 1965). Findings revealed significant differences between EdD programs and between EdD and PhD programmatic features. This data provides valuable insight for prospective students, informs EdD improvement, and urges consistency or standardization for clarity, integrity, and advancement in the field (Fisher et al., 2020; McMahon et al., 2020; Schafer & Giblin, 2008). [ABSTRACT FROM AUTHOR]

The burning of incense for home use is a widespread practice that has been shown to have significant negative impacts on human health and air quality. However, there is a lack of understanding regarding its emission profiles and associated health risks. To address this knowledge gap, we utilized a state-of-the-art thermal desorption comprehensive two-dimensional gas chromatography-mass spectrometer (TD-GC×GC-MS) to (semi-)quantify the emission factors (EFs) 317 volatile compounds and thoroughly investigate the organic profiles of incense burning smoke across a full-volatility range. Results showed that toluene (70.8 ± 35.7 µg g-1) is the most abundant compound in incensing-burning smoke, followed by benzene, furfural, and phenol. Phenol, toluene, furfural, 2-furanmethanol, benzene, and benzyl alcohol are the main contributors to ozone and secondary organic aerosol (SOA) estimation. Intermediate volatility organic compounds (IVOCs) accounted for 19.2% of the total EFs, but 40.0% of the estimated SOA. Additionally, a novel pixel-based method, combined with aroma analysis, revealed that furfural can act as a key tracer of incense burning, and is responsible for the distinctive flavor of incense smoke. High bioaccumulation potential (BAP) assessment using pixel-based partition coefficient estimation revealed that acenaphthylene, dibenzofuran, and phthalate esters (PAEs) are chemicals of high-risk concern and warrant further control. Our results highlight the critical importance of investigating home-use incense burning and provide new insights into the health impacts of incense burning smoke by novel approaches. [ABSTRACT FROM AUTHOR]

Research from numerous studies worldwide consistently shows that integrating social emotional learning (SEL) development into the structures and practices of schools is a path to creating safe, supportive, and inclusive environments. Researchers developed and validated an instrument to examine teachers' perceptions of SEL needs in their schools; their knowledge, skills, training, and experiences with SEL in their classrooms; and barriers to implementing practices or receiving professional development. A pilot study was conducted to assess the feasibility of the survey questionnaire, participant recruitment, and data collection and analysis processes. This paper describes the pilot testing process to ensure methodological rigor and content and face validity of the instrument before commencing the main research project surveying PK-12 teachers in Florida. This tool can be used in multiple sites and contexts to assess readiness and barriers to SEL program implementation, providing formative feedback for school leaders, curriculum developers, and teacher educators. [ABSTRACT FROM AUTHOR]