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Linear guide rail blocks are used in linear slide rail accessories to scrape off oil stains in the rails, installed on the front and rear ends of the slider. They are also used in milling machines, lathes, automated machines, robotic arms, electronic instruments, and so on. At present, the industry relies on manpower to carry out the quality inspection of this rail block which is difficult to standardize. Thus, automatic and digital deep learning inspection technology is introduced for the inspection. To understand the suitability of deep learning techniques applied to the linear guide block inspection process, we adopt the convolutional neural network model architecture and use the Xception model. In model training, the training effect is improved by amplifying the image method and testing many different defects. Through the Xception model, the training accuracy is about 98.7% after 30 epochs, the validation accuracy is about 97.4%, and the test accuracy is about 91.8%.

This study analyzed the role of G1 to S phase transition 1 protein (GSPT1) in promoting progression of liver cancer cells.A bioinformatics database was used to analyze the expression levels of GSPT1 in liver cancer tissues and the prognosis of patients. Subsequently, Western blotting and quantitative PCR were used to verify the expression levels of GSPT1 between normal hepatocytes and hepatoma cells. We used a CRISPR/Cas9 system to construct knockouts of GSPT1 in HepG2 and HCCLM9 liver cancer cells. The effect of GSPT1 on liver cancer cell migration and invasion was analyzed using flow cytometry, migration, and tumor formation assays.The Cancer Genome Atlas Liver Hepatocellular Carcinoma dataset indicated that GSPT1 expression was upregulated in liver cancer cell lines, and patients with liver cancer had poor prognosis. Knockout of GSPT1 in cells significantly inhibited tumor proliferation, cell migration, and growth in vivo.In this study, we found that GSPT1 promotes the migration and invasion of liver cancer cells.

Background Aberrant post transcriptional modifications of RNA, such as 5-methylcytosine modifications, have been frequently observed in breast cancer (BC). The M5C core catalytic enzyme NSUN2 gene was found to be co-amplified with the oncogenic transcription factor MYC in BC. However, the function of m5C modification in the immune response and metabolism to BC remains unknown. Therefore, we aimed to investigate the crosstalk between NSUN2 and MYC in BC microenvironment. Methods We investigated the function of NSUN2 in breast cancer using vivo and vitro assays. Next, the co-occurrence of NSUN2 and MYC in BC were tested by cBioPortal analysis. Co-immunoprecipitation and mass spectrometry were performed to determine the interact between NSUN2 and MYC. Then, correlation between the expression of NSUN2 and BC microenvironment were explored by CAMOIP, PCR and western-blotting. Additionally, RNA sequencing (RNA-Seq), Gene Set Enrichment Analysis (GSEA) were used to investigate the underlying mechanism of NSUN2 in BC. Results In the current study, increased expression of NSUN2 was observed in BC tissue compared to that in the adjacent normal tissue. Elevated NSUN2 levels were associated with poor prognosis in patients with BC. NSUN2 knockout (KO) suppressed cell proliferation, migration, and invasion; induced apoptosis in vitro; and repressed tumor growth in vivo. Subsequently, we determined NSUN2 can interact with MYC protein, and found that immune infiltration and glycolysis were increased in the NSUN2 KO BC cell line. In addition, RNA sequencing demonstrated that the differentially expressed genes in BC cells after NSUN2 KO were significantly enriched in the negative regulation of immune processes and metabolic processes. Conclusions Overall, NSUN2 overexpression drove immunosuppression and promoted BC progression. NSUN2 can interact with MYC protein, thereby being involved in the functional regulation of MYC target genes. Our findings suggest that NSUN2 may act as a potential therapeutic target for BC in the context of MYC amplification.

3,6-dichlorobenzo[b]thiophene-2-carboxylic acid (BT2) is a benzothiophene carboxylate derivative that can suppress the catabolism of branched-chain amino acid (BCAA)-associated mammalian target of rapamycin complex 1 (mTORC1) activation. Previous studies have demonstrated the therapeutic effects of BT2 on arthritis, liver cancer, and kidney injury. However, the effects of BT2 on ulcerative colitis (UC) are unknown.To investigate the anti-UC effects of BT2 and the underlying mechanism.Mouse UC models were created through the administration of 3.5% dextran sodium sulfate (DSS) for 7 d. The mice in the treated groups were administered salazosulfapyridine (300 mg/kg) or BT2 (20 mg/kg) orally from day 1 to day 7. At the end of the study, all of the mice were sacrificed, and colon tissues were removed for hematoxylin and eosin staining, immunoblot analyses, and immunohistochemical assays. Cytokine levels were measured by flow cytometry. The contents of BCAAs including valine, leucine, and isoleucine, in mouse serum were detected by liquid chromatography-tandem mass spectrometry, and the abundance of intestinal flora was analyzed by 16S ribosomal DNA sequencing.Our results revealed that BT2 significantly ameliorated the inflammatory symptoms and pathological damage induced by DSS in mice. BT2 also reduced the production of the proinflammatory cytokines interleukin 6 (IL-6), IL-9, and IL-2 and increased the anti-inflammatory cytokine IL-10 level. In addition, BT2 notably improved BCAA catabolism and suppressed mTORC1 activation and cyclooxygenase-2 expression in the colon tissues of UC mice. Furthermore, high-throughput sequencing revealed that BT2 restored the gut microbial abundance and diversity in mice with colitis. Compared with the DSS group, BT2 treatment increased the ratio ofOur results indicated that BT2 significantly ameliorated DSS-induced UC and that the latent mechanism involved the suppression of BCAA-associated mTORC1 activation and modulation of the intestinal flora.

A high-speed (HS) permanent magnet (PM) synchronous motor (HSPMSM) with a carbon fiber-reinforced plastic (CFRP) protective sleeve in the surface-mounted rotor was explored in this study. In view of retaining the PMs at HS operations, the high-strength CFRP sleeve was designed on the basis of a process that could be summarized as follows. First, a multi-physics analysis of the rotor was conducted. The requirements to the CFRP sleeve were obtained from electromagnetic analysis, loss analysis, heat flow coupling computational fluid dynamics analysis, structural analysis, and rotor dynamics analysis. Second, the CFRP sleeve was designed by theoretical analysis. From the results, the thickness values of the carbon fibers in the circumferential and helical directions were chosen to be 4 and 1 mm, respectively, while the helical angle of carbon fibers was chosen to be 70°. Then, a plate-shaped CFRP product and a cylindrical CFRP product were fabricated to verify the strength and stiffness of the CFRP sleeve. The performance test results show that the hoop strength and elastic modulus and the axial strength and elastic modulus of the plate-shaped CFRP products at 20 °C are 1963 MPa @ 156 GPa and 550 MPa @ 36 GPa, respectively. The hoop strain and axial strain of the cylindrical CFRP product under 35 MPa are about 3000 and 1900 μe, which meet the design requirements of the HSPMSM.

Considering the disadvantages of the traditional development pattern in an embedded control system for an inverter, that is, the single-process thinking and separation of software and hardware, a novel method, which is a model-based design, for developing a double closed-loop control system for the diode-clamped three-level inverter was proposed. System control models, including the PWM control algorithm model, the voltage control model, the neutral-point potential balancing model, and the frequency control model, were built with the MATLAB platform. The code-generation capacity and the operation effect were verified through a series of tests. The inverter with diode clamp and neutral-point potential control was developed. Codes were generated automatically and downloaded to the eZdsp28335 control chip. Experimental waveforms of the phase voltage, line voltage and current were analyzed under regulating the voltage and frequency. The experimental results demonstrate that the models and the generated codes are correct. Further studies have proven the feasibility of the system development model.

Background: The early diagnosis and intervention of esophageal squamous cell carcinoma (ESCC) is particularly important due to poor prognosis and the lack of effective therapies. This study aims to systematically describe the cellular atlas of human intramucosal ESCC.Methods: Five paired samples of intramucosal ESCC, para-ESCC esophageal tissue from endoscopically resected specimen, and peripheral blood mononuclear cells were adopted for scRNA-seq analysis. A computational pipeline scMetabolism was applied to quantify the metabolic diversity of single cells.Results: A total of 164,715 cells were profiled. The abundance of CD8+ TEXs , Tregs and PD1+CD4+T cells suggested an exhausted and suppressive immune microenvironment. Several genes in immune cells such as CXCL13 and CXCR5 in PD1+CD4+ T cells and PADI4 in Mono2 cells were identified as new biomarkers for poor prognosis. Epithelial cells exhibited high intra-tumor heterogeneity and two evolutional trajectories during ESCC tumorigenesis initiated from proliferative cells, and then through an intermediate state, to two different terminal states of normal differentiated epithelial cells or malignant cells, respectively. Intercellular interaction analysis based on ligand-receptor pairs revealed malignant cells interacting with CAFs via the MDK-NCL pathway, which is an important hallmark in the change of tumor microenvironment, and serves as a sign of CAF activation to stimulate downstream pathways for facilitating tumor invasion, and therefore suggesting a potential early biomarker of ESCC progression.Conclusions: This study demonstrates the changes of cell subsets and transcriptional levels in human intramucosal ESCC, providing unique insights into the development of novel biomarkers and potential intervention strategies.

Zika virus (ZIKV) is associated with severe birth defects and Guillain-Barré syndrome and no approved vaccines or specific therapies to combat ZIKV infection are currently available. To accelerate anti-ZIKV therapeutics research, we developed a stable ZIKV GFP-reporter virus system with considerably improved GFP visibility and stability. In this system a BHK-21 cell line expressing DC-SIGNR was established to facilitate the proliferation of GFP-reporter ZIKV. Using this reporter virus system, we established a high-throughput screening assay and screened a selected plant-sourced compounds library for their ability to block ZIKV infection. More than 31 out of 974 tested compounds effectively decreased ZIKV reporter infection. Four selected compounds, homoharringtonine (HHT), bruceine D (BD), dihydroartemisinin (DHA) and digitonin (DGT), were further validated to inhibit wild-type ZIKV infection in cells of BHK-21 and human cell line A549. The FDA-approved chronic myeloid leukemia treatment drug HHT and BD were identified as broad-spectrum flavivirus inhibitors. DHA, another FDA-approved antimalarial drug effectively inhibited ZIKV infection in BHK-21 cells. HHT, BD and DHA inhibited ZIKV infection at a post-entry stage. Digitonin was found to have inhibitory activity in the early stage of viral infection. Our research provides an efficient high-throughput screening assay for ZIKV inhibitors. The active compounds identified in this study represent potential therapies for the treatment of ZIKV infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12250-020-00316-0) contains supplementary material, which is available to authorized users.

The high-temperature fracture parameter Ct is an important parameter that is used to predict the service life and assess the safety of high-temperature structures in the entire creep stage. Therefo...

To investigate the clinical effect of double mini-locking plates in the treatment of ulna olecranon fractures.From March 2017 to May 2020, 19 patients with olecranon fractures were treated with double mini locking plates, including 12 males and 7 females, aged from 20 to 75 years old with an average of (40.50±7.62) years old;10 patients had the injuries on the left side and 9 patients on the right side. All the 19 patients were fresh closed fractures without ulnar coronoid process fracture, elbow dislocation and other injuries. The fracture healing time and complications were recorded, and the clinical efficacy was evaluated by Mayo elbow performance score (MEPS) before operation and 12 months after operation.All the 19 patientswas followed up, and the duration ranged from 12 to 17 months with an average of (13.51±3.17) months. Postoperative follow-up showed all fractures healed. Fracture healing time ranged from 2 to 6 months, with an average of(3.77±1.24) months. There was no internal fixation fracture, screw loosening, infection, internal fixation irritation, heterotopic ossification, elbow stiffness and other complications occurred. The MEPS score of affected elbow at 12 months after operation was 91.26±3.87, which was significantly different from that before operation 56.18±9.56 (It is a reliable fixation method to treat olecranon fracture with double mini locking plate. The incision lengh is small and the fracture fixation is reliable. Elbow joint function exercise can be performed early after operation. Postoperative internal fixation has less skin irritation and satisfactory elbow joint function recovery.

Cavitating jets have been widely studied for over a century, but despite the extensive literature on this subject, the implementation of cavitating jets in many industries is still very limited due to technical challenges. The main purpose of the present paper is to provide recommendations on using the cavitating jets based on a comprehensive literature review on the erosion mechanism in these jets. Self-resonating jets are extensively discussed in the present paper due to their importance in amplifying the erosion effect of cavitating jets. The influence of different jet nozzle geometric parameters and the operating conditions of the cavitating jet flow on the erosion mechanism is also discussed. Finally, well drilling in addition to multiple other industrial applications of cavitating jets are examined.

Purpose: This study aims to reveal the relationship between RNA N6-methyladenosine (m6A) regulators and tumor immune microenvironment (TME) in breast cancer, and to establish a risk model for predicting the occurrence and development of tumors.Patients and methods: In the present study, we respectively downloaded the transcriptome dataset of breast cancer from Gene Expression Omnibus (GEO) database and The Cancer Genome Atlas (TCGA) database to analyze the mutation characteristics of m6A regulators and their expression profile in different clinicopathological groups. Then we used the weighted correlation network analysis (WGCNA), the least absolute shrinkage and selection operator (LASSO), and cox regression to construct a risk prediction model based on m6A-associated hub genes. In addition, Immune infiltration analysis and gene set enrichment analysis (GSEA) was used to evaluate the immune cell context and the enriched gene sets among the subgroups.Results: Compared with adjacent normal tissue, differentially expressed 24 m6A regulators were identified in breast cancer. According to the expression features of m6A regulators above, we established two subgroups of breast cancer, which were also surprisingly distinguished by the feature of the immune microenvironment. The Model based on modification patterns of m6A regulators could predict the patient’s T stage and evaluate their prognosis. Besides, the low m6aRiskscore group presents an immune-activated phenotype as well as a lower tumor mutation load, and its 5-years survival rate was 90.5%, while that of the high m6ariskscore group was only 74.1%. Finally, the cohort confirmed that age (p < 0.001) and m6aRiskscore (p < 0.001) are both risk factors for breast cancer in the multivariate regression.Conclusion: The m6A regulators play an important role in the regulation of breast tumor immune microenvironment and is helpful to provide guidance for clinical immunotherapy.

This paper proposes a dual-band circularly polarized (CP) rectangular dielectric resonator antenna (DRA) for 5G millimeter wave wireless communications. The proposed DRA consists of a novel modified cross-flower exciting (MCF) slot and a rectangular ceramic dielectric resonator (DR). With the help of the compact MCF feeding slot, the fundamental modes TEδ11x/TE1δ1y and higher-order modes TEδ13x/TE1δ3y of the DR can be excited to achieve CP dual-band characteristics. In addition, the MCF slot works as a radiator at 26 GHz and 39 GHz to enhance the DRA bandwidth. More importantly, these two operational bandwidths can be controlled independently at the lower and upper bands. The proposed DRA is designed, fabricated and measured. The measured results show that the proposed dual-band CP DRA achieves 3 dB axial-ratio (AR) bandwidths of 19.8% and 7.8% with peak gains of 5.62 dBic and 6.74 dBic for the lower and upper bands.

The eukaryotic release factor 3a (eRF3a), a member of the eukaryotic peptide chain release factor family, is overexpressed in several types of cancer. This study aims to investigate the biological role and mechanism of eRF3a in the progression of liver cancer.Western blotting and RT-qPCR were used to detect the expression level of eRF3a in normal liver cells and liver cancer cells. The cell transfection experiments were performed to overexpress eRF3a levels in liver cancer cells HCCLM9 and Huh7, and then cell cycle and apoptosis experiments, Cell Counting Kit-8 (CCK8), plate cloning, and Transwell experiments were done to evaluate the function of eRF3a in the progression of liver cancer. The Western blotting was done to explore the mechanism of eRF3a promoting the development of liver cancer. Western blotting and RT-qPCR were used to detect the expression level of eRF3a in normal liver cells and liver cancer cells. The cell transfection experiments were performed to overexpress eRF3a levels in liver cancer cells HCCLM9 and Huh7, and then cell cycle and apoptosis experiments, Cell Counting Kit-8 (CCK8), plate cloning, and Transwell experiments were done to evaluate the function of eRF3a in the progression of liver cancer. The Western blotting was done to explore the mechanism of eRF3a promoting the development of liver cancer.eRF3a was significantly highly expressed in liver cancer cells, and its expression level was negatively correlated with the clinical prognosis of patients. In addition, in vitro experiments showed that eRF3a could promote the proliferation and migration of liver cancer cells through the ERK and JNK signaling pathways.This study suggests that eRF3a may be a potential prognostic marker for liver cancer and act as an oncogene by activating JNK and ERK signaling; therefore, eRF3a may be a new target for the treatment of liver cancer.

To investigate the expression of long non-coding RNA (lncRNA) H19 in mouse GC-1 cells in vitro and its effects on the proliferation and apoptosis of GC-1 cells.We established an in vitro hypoxia-reoxygenation model in GC-1 cells and detected the expression of lncRNA H19 in the GC-1 cells at different time points of reoxygenation injury by qRT-PCR. We determined the effects of silencing lncRNA H19 on the proliferation and apoptosis of the GC-1 cells by MTT and flow cytometry, the expressions of apoptosis-related proteins Bax and caspase-3 in the GC-1 cells by Western blot, and the expressions of microRNA-203a and PTEN by qRT-PCR and Western blot, respectively.With the prolonging of the time of reoxygenation injury, the expression of lncRNA H19 was increased significantly in the GC-1 cells and peaked at 3-hour hypoxia and 12-hour reoxygenation, but that of microRNA-203a markedly decreased. Silencing lncRNA H19 enhanced the proliferation and inhibited the apoptosis of the GC-1 cells, and up-regulated the expression of microRNA-203a and down-regulated that of PTEN in the GC-1 cells.LncRNA H19 is highly expressed in GC-1 cells in vitro, which may influence the proliferation and apoptosis of GC-1 cells by regulating the microRNA-203a /PTEN signaling pathway.

Background Circadian genes have been suggested to play an important role in lung pathology. However, it remains unknown whether polymorphisms of these genes are associated with chronic obstructive pulmonary disease (COPD). Here, we aimed to investigate the association of circadian genes polymorphisms with COPD in a case-control study of 477 COPD patient and 323 control Han Chinese persons. Methods Genotyping assays were carried out for nine single nucleotide polymorphisms (SNPs) from five circadian genes (PER3, CLOCK, RORB, BMAL1 and CRY2) that were previously identified in lung pathology. Age, sex, BMI and smoking status and comorbidities were recorded for all subjects. Results No significant association was found in all SNP sites in overall subjects and no significant difference was found in age, sex, smoking status stratification analysis. Discussion The findings of this investigation indicated the effect of circadian genes polymorphisms on COPD susceptibility may only be small and possibly dependent on the subject factors, such as age and sex.

In order to realize the sustainable utilization of water resources and the sustainable development of economy, the evaluation index system of water use efficiency system and economic development system was constructed. Entropy weight method and comprehensive evaluation method were adopted to determine the index weights and conduct comprehensive evaluation for the two systems. The coupling coordination model was used to calculate the coupling degree, coordination degree and coupling coordination degree of the two systems. The annual coupling stage and coupling coordination intensity of the two systems were analyzed and determined. The results showed that the comprehensive evaluation values of both water use efficiency system and economic development system in Jinan City increased greatly from 2008 to 2017, and showed a changing trend of ups and downs. The two systems were in the antagonistic stage, and were gradually approaching the running-in stage, indicating that they were in the state of common development. The coupling coordination degree of the two systems gradually increased in waves, progressed from the moderately to the highly coordinated coupling. In the future, if the water control path appropriate to Jinan City can be explored actively, the water-saving kinetic energy of economic development can be increased constantly, and the linkage effect between economic development and water resources utilization can be given full play to, the two systems will be in the orbit with the benign interaction and healthy harmonious development.

In order to achieve the sustainable utilization of water resources and the sustainable development of the economy, an evaluation index system for water-use efficiency and economic development systems was constructed. The entropy weight and the comprehensive evaluation methods were adopted to determine the index weights and conduct a comprehensive evaluation for the two systems. A coupling coordination model was used to calculate the coupling degree, coordination degree, and coupling coordination degree of the two systems. The annual coupling stage and coupling coordination intensity of the two systems were analyzed and determined. The results showed that the comprehensive evaluation values of both the water-use efficiency system and the economic development system in Jinan increased greatly from 2008 to 2017, and showed a changing trend of ups and downs. The two systems were in the antagonistic stage, and were gradually approaching the running-in stage, indicating that they were in the state of common development. The coupling coordination degree of the two systems gradually increased in waves, progressing from moderately to highly coordinated coupling. In the future, if the water control path appropriate to Jinan can be explored actively, the water-saving kinetic energy of economic development can be increased constantly, and the linkage effect between economic development and water resource utilization can be given full play. The two systems will then be in sync, enjoying a benign interaction and a healthy harmonious development.

Energy poverty refers to the lack of adequate, affordable, reliable, high-quality, safe and environmental-friendly energy services to support economic development and the lives of residents. It has become one of the major challenges facing the current world energy system. It is an urgent problem for China to solve about how to make clean energy available and affordable to the public. Based on the field survey data of Xuanhan County, this paper analyzed the energy poverty situation of rural families by constructing a comprehensive evaluation system of energy poverty. Furthermore, this study also found that energy poverty has had a significant negative impact on the average education level, health and indoor air of local rural families. Finally, based on the empirical results and the interviews with the respondents, this study put forward appropriate policy suggestions for alleviating local energy poverty.