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To proceed the clinical evaluation of DNA microarray for thalassemia gene detection.Peripheral blood samples of 166 thalassemia gene test subjects were collected and tested for thalassemia genes by microarray chip method and Gap-PCR method combined with PCR-reverse dot blot hybridization method according to double-blind control test. The specificity, sensitivity, positive predictive value, negative predictive value, and total coincidence rate of the microarray chip method were evaluated. When the two methods were inconsistent, multiplex ligation dependent probe amplification (MLPA) was used to verify the deletional α-thalassemia.Compared with Gap-PCR method, specificity, sensitivity, positive predictive value, negative predictive value, Youden index, and total coincidence rate of microarray chip method was 100% (70/70), 96.88% (93/96), 100% (93/93), 95.89% (70/73), 0.969, and 97.59% (162/166), respectively, while compared with PCR-reverse dot blot hybridization method was 100% (125/125), 100% (41/41), 100% (41/41), 100% (125/125), 1, and 100% (166/166), respectively.The microarray chip method for α-thalassemia gene detection shows the advantages of high specificity, sensitivity, and throughput.微阵列芯片法在α-地中海贫血检测中的方法学评价.对微阵列芯片法用于地中海贫血基因检测进行临床评价.收集166份地中海贫血基因受检者的外周血样本，按照双盲对照试验，分别应用微阵列芯片法和Gap-PCR法联合PCR-反向点杂交法对各样本进行地中海贫血基因检测，评价微阵列芯片法的特异性、灵敏度、阳性预测值、阴性预测值及总符合率。两种方法不一致时，对于缺失型α-地中海贫血采用多重连接依赖探针扩增（MLPA）法进行验证.微阵列芯片法与Gap-PCR法比较，特异性为100%（70/70），灵敏度为96.88%（93/96），阳性预测值为100%（93/93），阴性预测值为9589%（70/73），约登指数为0.969，总符合率为97.59%（162/166）；与PCR-反向点杂交法比较，其特异性为100%（125/125），灵敏度为100%（41/41），阳性预测值为100%（41/41），阴性预测值为100%（125/125），约登指数为1，总符合率为100%（166/166）.微阵列芯片法用于α-地中海贫血基因检测具有特异性高、灵敏度高、高通量等优点.

To perform dried blood spots thalassemia gene detection in patients with positive blood phenotypes by microarray technology, and evaluate its value in clinical detection.DNA samples were extracted from dried blood spots of 410 patients. Microarray technology was used to detect 3 deletion and 3 non-deletion types of α-thalassemia and 19 β-thalassemia point mutations which were common gene mutions in China.There were 357 positive cases in all the 410 tested samples with the positive rate 87.07%, among which 299 cases (72.93%) carried deletion or point mutations of α-thalassemia, 29 cases (7.07%) carried point mutations of β-thalassemia and 29 cases (7.07%) carried gene mutations of complex αβ-thalassemia syndrome. The mutations of α-thalassemia were involved with --The most common genetic mutations are --微阵列芯片法在地中海贫血患者基因突变检测中应用的研究.应用微阵列芯片技术对血液学表型分析阳性的患者干血斑进行地中海贫血基因检测，评价其在临床检测中的价值。.对410例患者的干血斑样本进行DNA提取，采用微阵列芯片技术对中国人常见的3种缺失型及3种非缺失型α-地中海贫血、19种β-地中海贫血点突变进行基因检测。.410例样本中，共检出阳性357例，总阳性率为87.07%。单纯α-地中海贫血基因缺失和点突变的样本有299例，占72.93%（299/410），其中177例为广西柳州地区α、β地中海贫血基因主要突变类型分别为

This article presents a synthesis technique for the planar Watt-I six-bar mechanism with a coupler point passing through three or four acceleration poles. The parametric equations for the loci of t...

To understand the pathological changes of tissues and organs in Microtus fortis infected with Schistosoma japonicum for further insight into the biological dynamic variation and explore immune mechanisms of M. fortis against S. japonicum infection.The healthy M. fortis and mouse were infected with cercariae of S. japonicum through abdominal skin. On the day 12, 20 and 40, the M. fortis and mouse were sacrificed by cervical dislocation and dissected. The liver and kidney slices of M. fortis and mouse of the different groups were prepared and stained with hematoxylin eosin stain (HE) by conventional techniques, respectively.We did not find obvious lesions on the internal organs of mouse 12 d and 20 d after the infection and the lesions of M. fortis 40 d after the infection. However, white nodules appeared on the liver, kidney and spleen of the M. fortis 12 d and 20 d after the infection, and there were a lot of S. japonicum worms on pathological sections of inflamed liver and kidney tissues, and the boundary between worms and normal organization was clear. There were no parasites on pathological section of liver and kidney of mouse 12 d and 20 d after the infection.Among M. fortis, there are severe immune responses and the immune responses have an individual difference 12 days after S. japonicum infection.