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Griffith AI&BD is a technology company that uses big data platform and artificial intelligence technology to produce products for schools. The company focuses on primary and secondary school education support and data analysis assistance system and campus ARTIFICIAL intelligence products for the compulsory education stage in the Chinese market. Through big data, machine learning and data mining, scattered on campus and distributed systems enable anyone to sign up to join the huge data processing grid, and access learning support big data analysis and matching after helping students expand their knowledge in a variety of disciplines and learning and promotion. Improve the learning process based on large data sets of students, and combine ai technology to develop AI electronic devices. To provide schools with the best learning experience to survive in a competitive world.

The intelligent glasses are similar to the smart phone, which possess independent operational system. And it can use diverse functions and applications by installing software, Meanwhile the intelligent glasses also are regarded as one of popular intelligent equipment in the resent years. The intelligent glasses are in an early stage of development, So there are some core hardware and application field, which still be researching and exploring continually, But its development will be quite rapid and the scale of market also is expanding continually in near future. The intelligent glasses have a more higher requirement for the quality of data transmission. With the advent of 5G time, The experience and significance of intelligent glasses will be increase sharply.

Green manure can be used as a substitute for chemical fertilizer without reducing rice yield. We studied the responses of soil fertility and rice yield to different combinations of Chinese milk vetch (CMV; Astragalus sinicus L.) and chemical fertilizer in a subtropical double-rice cropping system. Our goal is to reduce chemical fertilizer use and decrease environmental contamination. Compared with the recommended rate of chemical fertilizer (CF), both early- and late-rice yields in the two treatments supplied with 15 and 22.5 Mg CMV ha−1 plus 60% CF (represented as 60A and 60B, respectively) showed no significant differences while the two treatments supplied with 30 and 37.5 Mg CMV ha−1 plus 60% CF (represented as 60C and 60D, respectively) showed significantly higher values. The sustainable yield index (SYI) values in the 60C and 60D treatments with double-rice croppong system were significantly higher than those in other treatments (P0.05). Therefore, a relatively high Chinese milk vetch incorporation rate (≥30 Mg ha−1) was more effective in improving the productivity and sustainability of paddy soil. The decreased coefficients of early-rice yield and the Chinese milk vetch incorporation rate in 2014 implied that the benefits of soil fertility and rice yield created by Chinese milk vetch input may decline after five years under a continuously high rate of Chinese milk vetch incorporation.

The influencing effect of pressure on structural stability and elastic properties of PbSe2 compound is mainly investigated by first-principles method and homogenization method of the Y parameter. The optimized structural parameters at zero pressure are a=b=6.446Å, c=7.887Å (GGA method) and a=b=6.316Å, c=7.651Å (LDA method), which has good agreement with the experimental and theoretical values. Our calculated lattice parameters and Se-Se bond length are in excellent agreement with experimental data. PbSe2 compound is energetically stable with a good alloying ability. The elastic constants are calculated, and then the bulk modulus, shear modulus, Young’s modulus, Poisson’s ratio and anisotropy factor are determined. Besides, Y parameter method is used to investigate changes of the Poisson ratio, Young’s and shear moduli of PbSe2 within different normal orientation crystal planes. Results show that: 1) Young’s modulus is about 48.37 GPa from GGA and 58.87 GPa from LDA by Reuss-Voigt-Hill estimation, which is averaged about 53.62 GPa; 2) The PbSe2 compound is ductile according to B/G ratio. The universal anisotropic index AU shows that PbSe2 exhibits a fairly high elastic anisotropy.

A previous study described a novel serine protease inhibitor 16 from Musca domestica (MDSPI16), which inhibited the elastase and chymotrypsin. It also exhibited a potential anti-inflammatory activity for acute lung injury (ALI), while its effects on ALI are yet to be elucidated. The present study aimed to investigate the effects and the underlying mechanisms of MDSPI16 on lipopolysaccharide (LPS)-challenged mice and bone marrow neutrophils. The ALI model based on the results of LPS-induced mice demonstrated that MDSPI16 markedly reduced the infiltration of inflammatory cells, protein exudation in lung tissues, and downregulated the level of interleukin-6 (IL-6), IL-1β and tumor necrosis factor-α (TNF-α). Furthermore, the LPS-stimulated mouse bone marrow neutrophils model was employed to determine the role of MDSPI16. The cytokine levels were quantified by both the enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR). Consequently, the expression of IL-6, IL-1β, and TNF-α was found to be inhibited by MDSPI16 in a dose-dependent manner. Moreover, MDSPI16 also inhibited the mouse neutrophils nuclear factor-κB (NF-κB) signaling pathway, c-Jun N-terminal kinase (JNK) signaling pathway, ERK1/2 and AP-1 signaling pathway in addition to the expression of iNOS and COX-2 proteins, which in turn, might alleviate the release of pro-inflammatory cytokines during ALI. Therefore, MDSPI16 could be proposed as a potential and novel drug therapy for ALI.

Aeromonas veronii is a major pathogenic bacterium in humans and animals. When it causes outbreaks, there are enormous economic losses to the aquaculture industry. An effective live attenuated vaccine strain, ΔhisJ, was obtained in our previous studies by gene knockout in Aeromonas veronii TH0426 using the suicide vector pRE112. Here, we evaluated whether the live attenuated vaccine ΔhisJ was suitable for prevention of Aeromonas veronii infection by injection and immersion in loaches. Compared with that of the TH0426 wild-type strain, the virulence of the live vaccine was significantly weakened. Vaccine safety assessment results also indicated that 1 × 107 CFU/mL live vaccine was safe and did not induce clinical symptoms or obvious pathological changes. Additionally, after challenging loaches with Aeromonas veronii TH0426, the relative percent survival of the IN3 injection group was 65.66%, and that of the IM group was 50.78%. Our data show that the live attenuated vaccine ΔhisJ can improve the immune protection rate of loaches. Furthermore, increased enzyme activity parameters (SOD, LZM, ACP, and AKP) in the skin mucus, increased enzyme activity parameters (SOD, LZM, ACP, AKP, and GPx) in the serum, increased specific IgM antibodies and cytokine IL-1β contents in the serum, and increased cytokine (IL-15, pIgR, IL-1β, and TNF-α) expression in the liver and spleen were observed. These data are the first to indicate that the live attenuated vaccine ΔhisJ is suitable for the development of a safe and effective vaccine against Aeromonas veronii infection in loach aquaculture.

International cooperation on emergencies is significant to the response of these emergencies. Firstly, the connotation and characteristics of international cooperation on emergencies were analyzed. Secondly, the typical types of emergencies in need of international cooperation, the types of countries involved in international cooperation and the typical types of international cooperation modes on emergencies were analyzed in order to explain the mechanism of international cooperation. Finally, some strategies of international cooperation on emergencies were proposed. The result shows that, in order to have a better international cooperation, international cooperation modes on emergencies should be determined according to the types of emergencies, the situations of countries and organizations involved in it.

M. avium subsp. paratuberculosis (MAP) is the causative pathogen of Johne&rsquo, s disease, a chronic granulomatous enteritis that principally affects ruminants and can survive, proliferate and disseminate in macrophages. MicroRNAs (miRNAs) are important regulators of gene expression and can impact the processes of cells. To investigate the role of miRNAs in monocyte-derived macrophages (MDMs) during MAP infection, we used high-throughput sequencing technology to analyze small RNA libraries of MAP-infected and control MDMs. The results showed that a total of 21 miRNAs were differentially expressed in MDMs after MAP infection, and 8864 target genes were predicted. A functional analysis showed that the target genes were mainly involved in the MAPK signaling pathway, Toll-like receptor signaling pathway, NF-kappa B signaling pathway and apoptosis. In addition, using a dual-luciferase reporter assay, flow cytometry, and a small interfering (si)RNA knockdown assay, the role of miR-150 in regulating macrophage apoptosis by targeting the programmed cell death protein-4 (PDCD4) was demonstrated. These results provide an experimental basis to reveal the regulatory mechanism of MAP infection and suggest the potential of miRNAs as biomarkers for the diagnosis of Johne&rsquo, s disease in bovines.

M. avium subsp. paratuberculosis (MAP) is the causative pathogen of Johne’s disease, a chronic granulomatous enteritis that principally affects ruminants and can survive, proliferate and disseminate in macrophages. MicroRNAs (miRNAs) are important regulators of gene expression and can impact the processes of cells. To investigate the role of miRNAs in monocyte-derived macrophages (MDMs) during MAP infection, we used high-throughput sequencing technology to analyze small RNA libraries of MAP-infected and control MDMs. The results showed that a total of 21 miRNAs were differentially expressed in MDMs after MAP infection, and 8864 target genes were predicted. A functional analysis showed that the target genes were mainly involved in the MAPK signaling pathway, Toll-like receptor signaling pathway, NF-kappa B signaling pathway and apoptosis. In addition, using a dual-luciferase reporter assay, flow cytometry, and a small interfering (si)RNA knockdown assay, the role of miR-150 in regulating macrophage apoptosis by targeting the programmed cell death protein-4 (PDCD4) was demonstrated. These results provide an experimental basis to reveal the regulatory mechanism of MAP infection and suggest the potential of miRNAs as biomarkers for the diagnosis of Johne’s disease in bovines.

Objective: To investigate the effect of LBP on differentiation and maturation of healthy human peripheral blood-derived dendritic cells cultured in different tumor microenvironment in vitro, and discuss the molecular and immunological mechanisms of LBP in treatment of tumor. Methods: In this study, we procured the peripheral blood-derived dendritic cells precursor cell by the Density gradient centrifugation method, and used the tumor-cell supernatant to prepare conditioned medium. The GM-CSF and IL-4 induced DCs precursor cell differentiation to DCs, the TNF-α promoted the immature DCs developed to mature DCs. In this way, we detected the influence of LBP on the expressions of surface molecules of DCs cultured in different environments, and especially on the role of related-immunity and NF-κB activity. Results: In LBP-treated group, the molecular phenotype of DCs, its capacity to stimulate allogeneic lymphocyte proliferation, and the levels of IL-12p70 and IFN-γ secretion were higher than the untreated group (p

Taking advantage of improving voltage stability L indicator, voltage level, active power loss to assess device reactive capability in overload situations. Considering the intermediate buses which connect the generator bus and load bus, the compound buses which involve the two types of buses, improving voltage stability L indicator is proposed. So device reactive capability could be calculated. The example shows the best reactive device is generator, and the second one is capacitor bank and the last one is transformer tap. Assessment of reactive device capability is beneficial and practical for operation stuff to take appropriate measures in order to transfer overload situations into normal state.

Jing Rui Chen,1–3 Jing Wei,1–3 Ling Yan Wang,1–3 Yan Zhu,1–3 Lan Li,1–3 Mary Akinyi Olunga,1–3 Xiu Mei Gao,1–3 Guan Wei Fan1–31Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin, People’s Republic of China; 2Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Ministry of Education, 3Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, People’s Republic of ChinaAim: To investigate the potential cardioprotective effects of QiShenYiQi Pill® (QSYQ) on myocardial ischemia/reperfusion (I/R) injury through antioxidative stress and mitochondrial protection.Methods and results: Sprague Dawley rats were pretreated with QSYQ or saline for 7days and subjected to ischemia (30minutes occlusion of the left anterior descending coronary artery) and reperfusion (120minutes). Cardiac functions were evaluated by echocardiogram and hemodynamics. Myocardial mitochondria were obtained to evaluate changes in mitochondrial structure and function, immediately after 120minutes reperfusion. Pretreatment with QSYQ protected against I/R-induced myocardial structural injury and improved cardiac hemodynamics, as demonstrated by normalized serum creatine kinase and suppressed oxidative stress. Moreover, the impaired myocardial mitochondrial structure and function decreased level of ATP (accompanied by reduction of ATP5D and increase in the expression of cytochrome C). Myocardial fiber rupture, interstitial edema, and infiltrated leukocytes were all significantly ameliorated by pretreatment with QSYQ.Conclusion: Pretreatment of QSYQ in Sprague Dawley rats improves ventricular function and energy metabolism and reduces oxidative stress via ameliorating multiple mitochondrial dysfunctions during I/R injury.Keywords: QSYQ, ischemia/reperfusion injury, energy metabolism, mitochondria