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Abstract Background Investigating the molecular mechanism of colorectal cancer (CRC), a common lethal malignancies worldwide, is of great clinical significance. Solute carrier family 25 member 19 (SLC25A19) is a member of the solute carrier family that contribute to cellular functions, including tumor biology. Recently, many studies have attention on uncovering the relationship of SLC25A19 with malignant cancers, but its precise involvement in the regulation of CRC has not been thoroughly understood. This study sought to uncover the role and mechanism of SLC25A19 in CRC development. Methods The GEPIA database and immunohistochemical staining were utilized to detect the expression of SLC25A19 in CRC tissues. The functional influences of SLC25A19 on CRC cell phenotypes were evaluated through a series of assays including celigo cell count, colony formation, CCK‐8, flow cytometry, wound healing, and transwell assays following knocking down SLC25A19. Subsequently, the xenograft tumor model was constructed to evaluate the effect of SLC25A19 on tumor growth in vivo. The underlying mechanisms of SLC25A19 silencing were investigated using the human phospho‐kinase array. Results This study demonstrated the upregulation of SLC25A19 in CRC and its significant correlation with unfavorable prognosis in CRC patients. Suppression of SLC25A19 resulted in significant inhibition of cell proliferation, colony formation, and cell migration, alongside a boost in cell apoptosis. In vivo experiments revealed that silenced SLC25A19 displayed reduced growth rates and formed smaller xenografts. Mechanistically, the p53 pathway was found to be upregulated by SLC25A19 knockdown and mediated the function of SLC25A19. Conclusions Consequently, SLC25A19 was identified as a novel molecule with key regulatory ability in CRC development.

A dry gas seal (DGS) is one of the key basic components of natural gas transmission pipeline compressors, and the sealing performance of a DGS dealing with complex multi-component pipeline natural gas is different from that dealing with conventional nitrogen medium. In this paper, a spiral groove DGS of the compressor in natural gas transmission pipeline systems is taken as the research object. The thermal hydrodynamic lubrication model of the DGS is established considering turbulence effect and choking effect. Based on the finite difference method, the temperature and pressure distributions and the steady-state performance of the DGS are obtained by simulating. The influence of unitary impurity compositions such as light hydrocarbon, heavy hydrocarbon, non-hydrocarbon, and their contents on the steady-state performance of the DGS is analyzed. The steady-state performance of the DGS dealing with multi-impurity natural gas such as in the West-East gas transmission is investigated under different operating conditions. The results show that turbulence had a significant effect on the DGS, while choking had a weak effect. Increasing the content of light hydrocarbon such as C2H6 and heavy hydrocarbon such as C5H10 resulted in an increase in the gas film stiffness, leakage rate, and the temperature difference between the inlet and outlet, while non-hydrocarbon, such as N2, reduced the temperature difference between the inlet and outlet. The greatest impact on seal performance was produced by the heavy hydrocarbon, followed by the light hydrocarbon, and the least was produced by the non-hydrocarbon.

Background/Aims The aim of this study was to analyze the chronological changes in postoperative complications in surgical ulcerative colitis patients over the past decade in China and to investigate the potential parameters that contributed to the changes. Methods Ulcerative colitis patients who underwent surgery during 2008–2017 were retrospectively enrolled from 13 hospitals in China. Postoperative complications were compared among different operation years. Risk factors for complications were identified by logistic regression analysis. Results A total of 446 surgical ulcerative colitis patients were analyzed. Fewer short-term complications (24.8% vs. 41.0%, P=0.001) and more laparoscopic surgeries (66.4% vs. 25.0%, P

In this paper, a structural form called double-sided spiral groove gas face seal is considered. Steady-state performances, such as film load-carrying capacity and film stiffness, of gas face seals with single-sided groove on the stator, single-sided groove on the rotor, and double-sided groove are compared. Introducing axial and angular excitations, coupled with gas lubrication, seal rings vibration, contact mechanics, and instantaneous changes in fluid film thickness, the complete dynamic behavior history is obtained. Two dynamic performance indicators, logarithmic decrement and period valley, are defined. The effects of groove depths and equilibrium film thickness on the dynamic performance of gas face seals with three different surface grooving methods are studied. Combined with the transient sealing performance and film dynamic coefficients, the mechanisms of several typical unstable conditions are analyzed. The results show that the double-sided grooving method has both high gas film load-carrying capacity and gas film stiffness when the equilibrium film thickness is relatively large. In addition, double-sided grooving has both advantages of high gas film damping of single-sided grooving on the stator and high gas film stiffness of single-sided grooving on the rotor, which can ensure stable operation under various operation conditions.

Neovascularization is critical to improve the diabetic microenvironment, deliver abundant nutrients to the wound and promote wound closure. However, the excess of oxidative stress impedes the healing process. Herein, a self-adaptive multifunctional hydrogel with self-healing property and injectability is fabricated through a boronic ester-based reaction between the phenylboronic acid groups of the 3-carboxyl-4-fluorophenylboronic acid -grafted quaternized chitosan and the hydroxyl groups of the polyvinyl alcohol, in which pro-angiogenic drug of desferrioxamine (DFO) is loaded in the form of gelatin microspheres (DFO@G). The boronic ester bonds of the hydrogel can self-adaptively react with hyperglycemic and hydrogen peroxide to alleviate oxidative stress and release DFO@G in the early phase of wound healing. A sustained release of DFO is then realized by responding to overexpressed matrix metalloproteinases. In a full-thickness diabetic wound model, the DFO@G loaded hydrogel accelerates angiogenesis by upregulating expression of hypoxia-inducible factor-1 and angiogenic growth factors, resulting in collagen deposition and rapid wound closure. This multifunctional hydrogel can not only self-adaptively change the microenvironment to a pro-healing state by decreasing oxidative stress, but also respond to matrix metalloproteinases to release DFO. The self-adaptive multifunctional hydrogel has a potential for treating diabetic wounds.

IntroductionLocal knowledge tourism encompasses the rich cultural heritage, historical narratives, and traditional practices of a specific destination. Despite its significance in enhancing the tourist experience, there is a dearth of research examining the subjective perceptions and values of visitors engaging in local knowledge tourism. Consequently, there is a pressing need to explore the composition of perceived tourist values in this unique context.MethodsDue to the exploratory nature of this research, a constructivist grounded theory and content analysis are applied to analyze the data.ResultsThis study identifies and conceptualizes five distinct dimensions of perceived values in local knowledge tourism: functional value, emotional value, social value, cognitive value, and self-actualization value. Furthermore, an 18-item scale is developed to measure these dimensions quantitatively.DiscussionThis research makes several significant contributions: (1) it expands the scope of perceived value research within the tourism domain and enhances our understanding of the tourist experience in local knowledge tourism; (2) it provides a reliable instrument for future quantitative investigations into the behavior and mindset of local knowledge tourists; and (3) it offers theoretical foundations and practical insights for destination managers seeking to develop tourism products tailored to the preferences and expectations of local knowledge tourists.

Metal seals play a crucial role in ensuring the dependable functioning of the bearing system in roller cone bits. Due to the intricate nature of downhole conditions, the microstructure design of sealing end faces, specifically the surface texture, is rarely used in metal seals. This study focuses on examining the applicability of surface texture technology in metal seals for cone bits to enhance the lubrication characteristics and friction and wear properties of liquid film between end faces. A multi-field coupling model of surface-textured metal seals was established and experimentally verified. The liquid film carrying the capacity and sealing performance of five different shapes of surface-textured end faces (circle, ellipse I (horizontal), ellipse II (vertical), triangle, and chevron) were investigated under varying rotational speeds and environmental pressures. The influences of texture parameters, including depth and area ratio, on the lubrication characteristics and friction reduction effects of surface-textured metal seals were investigated, and the microscopic wear morphology characteristics of the sealing end faces were analyzed. The results show that surface textures can effectively improve the liquid film state and comprehensive performance of metal seals. Specifically, triangle textures significantly enhance the hydrodynamic pressure effect and weaken the abrasive wear and adhesive wear of the end faces. However, when the environmental pressure is p ≥ 30 MPa, the surface textures gradually lose the advantages of dynamic lubrication but can still reduce friction. The proper texture depth and area ratio can achieve zero leakage (obtained theoretically) and minimal friction in metal seals.

Taking the mechanical seal of the submersible as the object, a transient coupled heat transfer model was established, and the deformation of the seal face under stable and alternating conditions was investigated by considering the thermal–mechanical coupling effect. The deformation characteristics of the seal face under two different alternative forms of amplitude and period were analyzed. The morphology and wear characteristics of the seal face were measured and discussed based on the pseudo-real test of the mechanical seal under alternating loads. The results showed that the seal face tended to be squeezed near the inner diameter and a tapered convergent gap appeared at the outer diameter of the seal face under alternating loads. The deformation of the seal face presented obvious alternating transient characteristics under alternating loads, and the deformation under alternating rotational speed was greater than that of alternating medium pressure. The deformation of the seal face increased with the amplitude of the alternating loads, while the period of the alternating loads had little influence on it. The wear mechanism of the seal face under alternating conditions was mainly abrasive wear, and the wear caused by alternating rotational speed is more significant.

By setting textures on the side walls of a rotor, based on SST k-ω turbulence and the mixture model, the effects of depth-to-diameter ratio, shape, and rotational speed on interface temperature are analyzed. Local Nu number, flow field in textures, and gas distribution are used to verify the conclusion. When rotational speed increases, there are three different stages on the surface: liquid-dominated, mixed two-phase, and gas-dominated. This leads to a big difference in heat transfer on the side wall and causes the temperature on the seal face to increase when cavitation is considered. The distribution of the gas phase is explained through drag reduction, which has a high correlation with the velocity gradient near the surface. For several common shapes, heat transfer enhancement of textures is compared under high speed. The key influencing factor is the depth-to-diameter ratio, which causes flow stratification and reduces heat transfer. Flow stratification leads to different results of maximum temperature on the seal face when cavitation is considered. Results show that at high speed, a deep, circular texture is better when cavitation does not occur, and a shallow triangular texture is recommended when cavitation occurs; a textured side wall can reduce the maximum temperature of the seal face by about 10 °C.

The film stiffness of gas-lubricated hydrostatic bearings and seals are considered to be enhanced remarkably by proper design of a diaphragm-type restrictor. A fluid-structure coupling numerical model by coupling iteration of small deflection bending differential equation of circular thin plate and one-dimensional Reynolds equation was established, and the influence of different parameters, including pressure and geometrical parameters, on the flow output characteristics of a diaphragm-type restrictor was investigated numerically and experimentally. The results show that the correctness of established fluid-structure coupling numerical model is verified well by comparing calculated values of diaphragm deformation and output flow characteristics to the measured values. The optimum values of diaphragm thickness is 0.6–0.8 mm and initial throttle clearance is 20–35 μm for stable operation of diaphragm-type restrictor, while the optimum value of back pressure strongly depends on the diaphragm thickness.

The rail inductance gradient is an important parameter of the electromagnetic launcher. The calculation of its value is important for the design of the launcher structure and for predicting the motion behavior of the armature. The current research on the inductance gradient analysis method of the electromagnetic rail launcher mostly does not take into account the effects of launcher size and current diffusion. This method cannot describe its dynamic characteristics, and it results in a large error compared with the actual launch. Therefore, the paper first establishes an electromagnetic rail launcher armature motion model to obtain the rail velocity skin depth under a U-shaped armature. Second, an analytical method for calculating the inductance gradient based on the dynamic skin depth of the rail is obtained, which takes into account the launcher size and velocity skin effect. Finally, the experimental results verify the correctness and accuracy of the method to achieve an accurate prediction of armature speed.

In order to solve the problems of insufficient precision of the armature velocity and inductance gradient in the process of finite element calculation of the electromagnetic track launcher, an improved dynamic conductivity electromagnetic-thermal coupling model is proposed to make the calculated results closer to the actual working condition. Firstly, the finite element analysis for the electromagnetic-thermal field is carried out. Then, considering the influence of dynamic conductivity on the armature velocity and inductance gradient, an improved dynamic conductivity electromagnetic-thermal coupling model based on finite element analysis is established, whose parameters are identified by the proposed PSO-GA hybrid algorithm. Moreover, the predicted values of armature velocity and inductance gradient are also obtained. Finally, the experimental platform of the electromagnetic track launcher is built to verify the improved model. By comparing the predicted value of the improved model with the experimental test value, it is found that the improved model can further reduce the calculated error from 5.79% to 1.18%, which provides a certain theoretical basis for the full true simulation of the electromagnetic track launch.

To study the influence of the laser power, scanning speed, and cleaning water content on the laser cleaning effect and obtain the best cleaning parameters, this paper conducted a simulation analysis of the laser cleaning process and carried out a pulse laser cleaning of porcelain insulators experiment to verify. The results show that the cleaning rate gradually increases as the laser power increases from 20 W to 25 W. As the scanning speed increases from 1000 mm/s to 2500 mm/s, the laser overlapping rate gradually decreases, and the cleaning takes the lead in increasing and then decreasing. The appropriate cleaning water content is conducive to laser cleaning; when the water content is 0.115 g, the cleaning efficiency reaches the highest value of 98.20%. When the laser power is 25 W, and the scanning speed is 2000 mm/s, the cleaning efficiency can reach the highest value of 96.87%. This paper shows that the reasonable choice of cleaning parameters can effectively clean the insulator surface filth and obtain a better surface morphology.

China has abundant shale gas resources with great exploration potential, and stage progress has been made in this aspect. The sedimentary environment and reservoir characteristics are important aspects of the study on shale gas accumulation. Previous studies have mostly been carried out from a “qualitative” perspective, but not from a “quantitative” one. There is a lack of comparative studies on “marine shales with different TOC contents”. This paper takes the marine shale of the first member of the Longmaxi Formation (Long 1 Fm) in southern Sichuan Basin, Southern China, as the research object. The core samples were taken to carry out analyses (mineral composition analysis, TOC content analysis, porosity analysis) and experiments (carbon dioxide and nitrogen adsorption experiments, high-pressure mercury intrusion, FIB-SEM, and FIB-HIM experiments). The element logging data were collected to conduct the quantitative comparison of genesis and pore structure characteristics of siliceous minerals in marine shale with different TOC contents in this area. The conclusions are as follows: first, a formula is used to calculate and determine whether there is excessive silicon; then the Al-Fe-Mn triangle diagram is used to analyze the genesis of excessive silicon, so as to quantitatively analyze the genesis of siliceous minerals in shale: the siliceous minerals of organic shale (1% < TOC 2%) are detrital genesis, and a small part (0–20%) are biogenic. Carbon dioxide and nitrogen adsorption experiments, as well as high-pressure mercury intrusion experiments are adopted to quantitatively characterize the whole-aperture pore structure characteristics. The pore development characteristics of different shale components are analyzed by combing FIB-SEM and FIB-HIM experiments. The organic-bearing shales in the target section of this study area mainly develop clay mineral pores (71%), and are dominated by macro-pores (57.3%) with a low number of pores, irregular-shaped pores, as well as poor storage capacity and connectivity; the organic-rich shales in the target section of this study area mainly develop organic pores (51%), and are dominated by micro-pores (32.1%) and mesopores (54%) that are large in number and elliptical-shaped, with good storage capacity and good connectivity. The results of this study help to improve the understanding of the pore size of marine shales, the origin of siliceous minerals in marine shales, and the pore structure characteristics of marine shales, which are of great theoretical and practical significance for improving the theory of shale gas formation and guiding the selection of shale gas sweet spot.

Electromagnetic forming is a novel technology in which a coil is used to generate a pulsed Lorentz force for shaping sheet metal pieces. However, the Lorentz force between the coil and the sheet metal is generally repulsive due to the Lenz's Law, which limits the practical application of this technology. To break this bottleneck, a dual-coil method is proposed to generate attractive Lorentz force, which is then used to form sheet metals. The theoretical principle of this method is presented, and an infinite element model is established to simulate the forming effect. The simulation results show that an A1060-O plate with a diameter of 140 mm and thickness of 1 mm can be attractively shaped under this method. The maximum axial displacement of the plate reaches 2.74 mm. Unfortunately, a dent exists in this situation. Further analysis indicates that this dent can be eliminated by increasing the inner and outer diameters of the coils. Further, it is found that when the capacitance value of the long pulse-width discharge circuit is set to 5.6 mF and the initial discharge voltage is set to 10.690 kV, the sheet metal attains the best shape.

Due to the fact that geometrical structure of tube electromagnetic bulging process with field shaper does not conform to the axisymmetric property, three-dimensional (3D) model is usually used to analyze the process which is complicated and time consuming. Therefore, on the basis of understanding the working principle of the field shaper, a two-dimensional (2D) model with current constraint is proposed in this paper to analyze the electromagnetic bulging process of a tube fitted with a field shaper. COMSOL finite element analysis software is used to develop three models for performance analysis and comparison, these are: conventional 3D model and the proposed 2D model with and without current constraint. Simulation results show that the induced eddy current density, magnetic flux density and electromagnetic body density of the two-dimensional model with current constraint are in good agreement with the results of the three-dimensional model. This provides an alternative and effective way to analyze the performance of the tube electromagnetic bulging with field shaper using a simple but yet reliable 2D model.

The attractive electromagnetic bulging is a novel technology which has unique advantages in the formation of small tube fittings. To generate the attractive electromagnetic force, a dual-frequency current method has been proposed in the existing research on the attraction bulging of the small tube fittings. This method, however, has a flaw that the tube fittings are subjected to a large repulsion electromagnetic force before the attraction, which will lead to a negligible effect on the final forming results. To solve this problem, a non-uniform solenoid coil structure is proposed in this paper to suppress the repulsion to improve the attraction. On this basis, a finite element model is built to analyze the process of an aluminum tube with an inner diameter of 20 mm and an outer diameter of 22 mm and the thickness of 1 mm. The influences of the coil structure on the magnetic induction intensity B, eddy current density Je and the stress of the coil have been studied. The results show that the expansion of the tube can be improved when the non-uniform solenoid coil is used. The maximum expansion in this dissertation is achieved with a 28 turns non-uniform solenoid, and its expansion uniformity standard deviation is only 0.156. These simulations have a significant outcome to the design of the coils and the application of electromagnetic bulging in small tube fittings.

Electromagnetic forming is a method of high-speed metal workpiece forming technology that uses electromagnetic force pulses generated from the interaction of the magnetic flux density and workpiece induced eddy current. The main challenge of this technology is the control of the eddy current and magnetic flux density to perform various forming processes. This paper is aimed at overcoming this challenge by introducing a new electromagnetic sheet forming method based on discretely driven rings. In this method, a set of discrete conducting rings are placed between the driving coil and the metal workpiece to enhance the shaping process of the workpiece. By changing the number and position of the rings, the induced eddy current can be adjusted, and different electromagnetic force distribution profiles can be realized. COMSOL software is used to develop an electromagnetic field-structure coupling model of the proposed electromagnetic forming method. The characteristics of the electromagnetic force distribution and its effect on the workpiece forming process are analyzed. Results show that the electromagnetic force distribution profile can be controlled by adjusting the rings physical parameters such as diameter, material, and position. The proposed method can effectively improve the performance of the conventional electromagnetic forming technology and has the potential for implementation in wide industry applications.

Electromagnetic tube expansion has been given much attention due to its superior cold forming properties. The traditional electromagnetic coil is of single structure, which results in substantial tube wall thinning and mechanical strength reduction. This shortcoming restricts further development of the electromagnetic tube expansion technology in various industrial applications. This paper proposes an axial-radial electromagnetic force bidirectional loading method to increase the axial fluidity of the material and eliminate the wall thinning issue that currently exists in the traditional method. Furthermore, the performances of three proposed structures for the axial compression electromagnetic tube expansion using three-coil, two-coil and one-coil are analyzed and compared. In this context, the electromagnetic force distribution and the wall thinning of the tube during the expansion process are analyzed based on finite element modelling. Results show that with the proposed modified structure of the traditional single coil, the axial electromagnetic force can be substantially increased which results in reducing the tube wall thinning significantly. The proposed technique in this paper paves the way for further development and more industrial applications of the electromagnetic expansion technology.

In the conventional electromagnetic tube expansion, tube wall thickness tends to decrease significantly while the workpiece is expanded. This paper is aimed at introducing a new technique using three-coil electromagnetic tube bulging with axial compression as an innovative solution for this issue. To validate the feasibility of the proposed technique and investigate the electromagnetic force distribution along the workpiece, COMSOL software is used to construct a detailed finite element analysis model for the proposed electromagnetic coupling model. Results show that the ratio of the axial and the radial electromagnetic forces increases linearly with the increase of the height and the outer diameter of the top and bottom coils. Furthermore, the tube deformation profile and the wall thickness are compared with two different coil structures. Comparison analysis reveals that for the same axial to radial electromagnetic forces ratio, the effect of prohibiting the wall thickness reduction using different coil structures is quite similar. The new method is expected to have a good potential in enhancing the tube-forming performance.

Based on Laplace transform and Hurwitz stability criterion, the system characteristic equation and algebraic stability criterion of the two degree-of-freedom angular free vibration of dry gas seal are derived. The effects of rotational inertia, stiffness and damping parameters on the angular free vibration stability of dry gas seal are analyzed by using the root locus method and the concept of the closed-loop dominant pole. The results show that the constraint condition of rotational inertia is the most demanding of all stability conditions. Both the angular main damping and main stiffness are not simply the larger the better, but there are preferred values in the interval greater than the stability threshold. The approach of the absolute value of the cross coefficient to zero is beneficial to the suppression of angular free vibration. There is a threshold of rotational inertia, which makes the cross stiffness change from having only the lower limit value to both the upper and lower critical values at the same time.

The main aim of the present research work was to demonstrate the antitumor and apoptotic activities of oxypeucedanin hydrate monoacetate isolated from Angelica dahurica against Caco-2 colon cancer cells. MTT cell viability assay along with clonogenic assays were used to study the effect of the compound on cell cytotoxicity and the colony forming tendency. Fluorescence microscopy using acridine orange/ethidium bromide was used to study the effects on apoptosis. In vitro wound healing assay and Western blotting were carried out to study effect on cell migration and PI3K-signalling pathway. The results revealed that oxypeucedanin hydrate monoacetate inhibited Caco-2 cell proliferation and decreased the number of cancer colony forming cells. This compound exerted anti-migratory effects in dose- and time-dependent manner. As compared to the untreated control, drug-treated cells showed significant inhibition of cancer cell migration. Oxypeucedanin hydrate monoacetate significantly down-regulated the expression of pAkt and pPI3K in Caco-2 cells.