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To investigate the effect of gap junction intercellular communication (GJIC) combined by connexin43 (Cx43) and its signal to the biobehavior of multiple myeloma (MM) cells, and its possible mechanism.The mesenchymal stem cell (MSC) cells were isolated and cultured from patients with MM and normal donors. The expression of connexin43 (Cx43) in MSC cells from different sources was detected by RT-PCR and Western blot. The side population (SP) cells were sorted by flow cytometry (FCM). The effect of MSC cells from different sources to the cell cycle, Cx43 expression, colony formation in vitro, stem cell related genes expression, cytokines secretion and chemoresistance in MM SP cells as well as with or without Cx43 inhibitor 18α-glycyrrhetinic acid (18α-GA) was observed.There was no significantly difference between the MSC isolated from normal donor and MM patients. Western blot showed that Cx43 expression in SP cells was up-regulated when the cells were incubated with MSC, and medium containing 18α-GA could partially inhibit it, moreover, it was more significant in MSC cells of MM patients. The ability of colony formation of SP cells in vitro was higher than those of MM cells and MM-MSC could promote the colony formation in a co-culture manner. The effect of MM-MSC to SP cells was down-regulated after 18α-GA was added. RT-PCR showed that there was several important stem cell-related genes including c-myc, Oct-4 Klf-4, and Sox-2 were found in RPMI 8226 cells, but those cells were up-regulated in SP cells (P0.001). Meanwhile, MM-MSC could up-regulate the expression of c-myc, Klf-4 and Sox-2 (P0.001), but down-regulate Oct-4 gene in the SP cells. The expression of those genes decreased after 18α-GA was added, but showed no significant difference (P0.05). Cytometry bead array assays showed that MM-MSCs could secrete high level of IL-6, but the levels of IL-6, IL-10 and TGF-β increased significantly when the MM-MSCs were co-cultured with SP cells (P0.05), especially the levels of IL-6 and IL-10 were significantly higher than cultured alone. There was no significant change in the levels of bFGF and IL-17 before and after co-cultured. The levels of IL-6, IL-10 and TGF-β in supernatant decreased significantly after GJ inhibitor 18α-GA was added. PI/Annexin V assay showed that MM cells were sensitive to bortezomib (BTZ)-induced apoptosis, but the sensitivity for SP cells was weaker. The ratio of cell apoptosis was 75.2%±0.77% and 8.12%±0.86% (P0.001), respectively. MM-MSC could down-regulate the cell apoptosis induced by BTZ, while the sensitivity of MM cells to BTZ could be partially recovered after GJ inhibitor was added.MSC derived from MM patients can enhance GJIC to maintain its "hematopoiesis" by up-regulating the expression of Cx43 in MM cells, and at the same time promote cell proliferation and drug recistance by secreting multiple cytokines, which finally contributes to the relapse of MM.连接蛋白43偶合对多发性骨髓瘤细胞生物学行为的影响.观察由连接蛋白43（Cx43）组成的细胞间隙连接通讯（GJIC）及其介导的信号对多发性骨髓瘤（MM）细胞生物学行为的影响，并探讨其可能机制。.分离、培养MM患者、正常人骨髓间充质干细胞（MSC）；采用RT-PCR及蛋白印迹检测不同来源MSC中Cx43的表达，流式细胞术分选MM侧群细胞（SP），直接共培养后观察不同来源MSC对SP细胞周期、Cx43表达、体外集落形成、干细胞相关基因表达、细胞因子分泌和耐药的变化以及加入GJIC抑制剂18α甘草次酸（18α-GA）的影响。.MM患者及正常人骨髓所获的MSC形态及表型无明显区别，蛋白印迹证实，这两种MSC均表达较高水平的Cx43，与SP细胞共培养后可上调其Cx43表达，18α-GA可部分抑制该作用，对来源于MM患者的MSC作用更为显著（P0.001）；SP细胞具较强的体外集落形成能力，MM-MSCs具促进作用，加入18α-GA后SP细胞体外集落形成能力下降；RT-PCR检测证实RPMI 8266细胞存在少量c-myc、Klf-4、Sox-2和Oct-4基因表达，但SP细胞亚群中该类基因明显上调（P0.001），MM-MSC可显著上调SP细胞c-myc、Klf-4和Sox-2基因的表达（P0.001），而下调Oct-4基因表达，加入GJ阻断剂后，上调的基因均有不同程度下调，但无明显差异（P0.05）；CBA分析结果显示，MM-MSC分泌的高水平白介素（IL），与SP细胞共培养后，其上清中IL-6、IL-10及转化生长因子-β（TGF-β）表达上调（P0.05），尤其是IL-6和IL-10较单独培养时显著上调（P0.01），碱性成纤维细胞生长因子和IL-17共培养前后则无明显变化，加入18α-GA后，上清中IL-6、IL-10和TGF-β水平均明显降低（P0.05）；PI/Annexin V检测证实，MM细胞对硼替佐米诱导的凋亡敏感，但SP细胞敏感性较差，凋亡率分别为75.2%±0.77%和8.12%±0.86%（P0.001），MM-MSC可显著减少硼替佐米介导的细胞凋亡 （P0.05），加入18α-GA可部分恢复MM细胞对硼替佐米的敏感性。.MM患者来源的MSC通过上调MM细胞Cx43表达，强化GJIC维持其“干”性，同时通过分泌多种细胞因子促进细胞增殖和耐药，是最终导致MM复发的原因之一。.

p-Nitrophenol is usually present in ammonia-rich wastewaters produced by some chemical plants. In this work, the response of anammox process to long-term p-nitrophenol stress was investigated. The changes in the efficiency, sludge characteristics, and microorganisms of the anammox system under different levels of p-nitrophenol stress were examined, and the potential stress mechanisms of p-nitrophenol on anammox were further speculated. The results showed that 10-50 mg/L p-nitrophenol had no obvious impact on nitrogen removal efficiency, but stimulated the secretion of more extracellular polymeric substances. 60 mg/L p-nitrophenol caused the nitrogen removal efficiency to decrease by 64.5% in 5 days. Long-term exposure to p-nitrophenol led to 8.6% reduction in Candidatus_Kuenenia abundance and 18.4%-35.9% decrease in the expression level of anammox bacterial functional genes. Molecular simulation indicated that p-nitrophenol could bind to key enzymes of anammox. This study provides new insights into the treatment of wastewater containing p-nitrophenol or phenol by anammox.

Growing evidence shows that cancer progression links with both heterogeneity of the tumor microenvironment and dysregulated activity of immune cells. Cancer-secreted exosomes are being recognized as indispensable mediators of the exchange cargo between cancer and immune cells. The M2-phenotype tumor-associated macrophages have the function of promoting tumor progression and drug resistance. Diffuse large B-cell lymphoma(DLBCL) is a highly heterogeneous and very common malignant non-Hodgkin's lymphoma. Here, we demonstrate that different subtype DLBCL cell-derived exosomes are internalized by macrophages, which can affect macrophages polarization. The mechanism of DLBCL-derived exosomes on macrophage polarization remains unclear currently. This study showed that DLBCL-secreted exosomes could induce the transformation of macrophages to a protumor M2-like phenotype, and block the drug-induced apoptosis of DLBCL cells in an indirect co-culture system. Different DLBCL-derived exosomes could change the phenotype of macrophages through the STAT3 signaling, which upregulated the expression of oncogenic genes and classical markers of M2-like phenotype macrophages, such as IL-10, CD206, and CD163. The addition of DLBCL-derived exosomes resulted in the activation of the STAT3 signaling pathway of M0/M2 macrophages in an indirect co-culture system. GP130 was highly enriched in DLBCL-derived exosomes, which triggered the activation of STAT3 of macrophages and subsequently induced the downstream targets such as BCL2, SURVIVIN, and BAX. The parallel changes of STAT3 and GP130 in macrophages confirmed that GP130 of DLBCL-derived exosomes promoted macrophage polarization by activating STAT3 signaling. Furthermore, all of these effects could be reversed by the GP130 inhibitor SC144. The data indicated that DLBCL-derived exosomes could trigger macrophages polarization into a pro-survival M2-like phenotype, which was at least partially through the GP130/STAT3 signaling pathway. Collectively, this study showed that DLBCL-derived exosomes could promote macrophages transformation to protumor M2-like phenotype in the tumor microenvironment.

Phenol is a biotoxic organic compound and found in large quantities in ammonia-rich wastewater discharged from coking and petrochemical industries. In this work, phenol was fed to the system of anaerobic ammonia oxidation (anammox), and the possible inhibitory mechanism was speculated using the characterization of granular sludge, analysis of microbial community and molecular docking simulations. The results showed that phenol (0-300 mg/L) did not significantly inhibit anammox. However, phenol did activate denitrification, which increased the nitrogen removal rate (NRR) by 0.94 kg N/(m

Exosomes are key mediators of intercellular communication and play a role in the pathogenesis and progression of cancer. Exosomes in circulating body fluids serve as molecular markers for cancer diagnosis. This study aimed to investigate the role of exosomal microRNA (miR)-1910-3p in breast cancer and determine its clinical diagnostic value. MiR-1910-3p promoted proliferation and migration of breast cancer cells in vitro and in vivo. In vitro, exosomes enriched in miR-1910-3p transferred miR-1910-3p to mammary epithelial cells and breast cancer cells, promoting proliferation and migration, inhibiting apoptosis, and inducing autophagy. In vivo, exosomes enriched in miR-1910-3p promoted the proliferation and migration of breast cancer cells. MiR-1910-3p downregulated myotubularin-related protein 3, activated the NF-κB and wnt/β-catenin signaling pathway, and promoted breast cancer progression. Serum miR-1910-3p in exosomes was an effective diagnostic marker that improved the sensitivity of breast cancer diagnosis when used in combination with the traditional tumor marker CA153. In conclusion, breast cancer cell-derived exosomes promoted the growth, metastasis, and autophagy of breast cancer cells by transferring miR-1910-3p. MiR-1910-3p in serum exosomes may serve as a novel molecular marker for breast cancer diagnosis.

Objective To observe the effect of curcumol on the biological behavior of multiple myeloma (MM) cells, thus studying its possible mechanisms for MM treatment. Methods Bone marrow mesenchymal stem cells (BMSCs) and multiple myeloma cell line 8226 (RPMI 8226) were taken as sub- jects, which were then divided into the RMPI 8226 group (cultured by RMPI 8226 alone) and the BMSCs + RMPI 8226 group (cultured by BMSCs and RMPI 8226). Curcumol in different concentrations (0. 1, 0.5, 1. 0 , 10.0 μg/mL) was added to cells in the two groups respectively. Cell proliferation, cell cycle, and ap' optosis induced by curcumol were examined by flow cytometry. The expressions of receptor activator of nuclear factor Kb ligand ( RANKL ) and osteoprotegerin ( OPG) were detected using reverse tran- scriptase-polymerase chain reaction (RT-PCR). Results Curcumol induced arrested cell cycle of RMPI 8226. The arrest of RMPI 8226 cell cycle was more obviously in the RMPI 8226 group than in the BMSCs + RMPI 8226 group. After curcumol treatment the cell proliferation of RPMI 8226 was significantly inhibited (P0. 01) and its apoptosis was increased (P0. 01). Co-cultured with BMSCs decreased curcumol in- duced apoptosis of RPMI 8226. Curcumol down-regulated the expression of osteogenic differentiation related gene RANKL, and up-regulated the expression of OPG. Conclusions Curcumol disturbed the cell cycle and induced apoptosis of RPMI 8226 cells. Curcumol up-regulated the expression of OPG as well as down-regulated the expression of RANKL. Co-culture with BMSCs could obviously inhibit curcumol in- duced apoptosis of MM cells, which might be associated with drug resistance of MM cells.

In this investigation, global DNA methylation patterns and the specific methylation status of 5 genes were studied in DNA from peripheral blood (PB) and impact on progression free survival (PFS) and overall-survival (OS) in patients with de novo or relapsed acute myeloid leukemia (AML) treated with decitabine-based regimens waas assessed. DNA was isolated from PB samples at the time of -1, 1, and 7 days of chemotherapy. Global methylation was determined by ELISA, and the CpG island DNA methylation profile of 5 genes using a DNA methylation PCR system. Our data demonstrated that patients with a high level of 5-mC had a poor prognosis after demethylation therapy and those who have low levels of 5-mC in PB achieved higher CR and better SO, but there was no significant correlation found between the 5-mC levels and other clinical features before treatment except the disease status. Higher methylation status of Sox2 and Oct4 genes was associated with differential response to demethylation therapy. A relatively low methylation percentage in one or both of these two genes was also associated with longer OS after decitabine based chemotherapy. We also suggest that global DNA and Oct-4/Sox2 methylation might impact on the pathogenesis of leukemia and play an important role in the initiation and progression. Moreover, dynamic analysis of 5-mC and Oct-4/Sox2 in peripheral blood nucleated cells of leukemia patients may provide clues to important molecular diagnostic and prognostic targets.

Background/Aims: Interferon regulatory factor 1 (IRF-1) has been shown to function as a transcriptional activator or repressor of a variety of target genes. However, its upstream, non-coding RNA-related regulatory capacity remains unknown. In this study, we focus on the miRNA-associated single nucleotide polymorphisms (SNPs) in the 3′untranslated region (UTR) of IRF-1 to further investigate the functional relationship and potential diagnostic value of the SNPs and miRNAs among Chinese gastric cancer (GC) patients. Methods: We performed a case-control study with 819 GC patients and 756 cancer-free controls. Genotyping by realtime PCR assay, cell transfection, and the dual luciferase reporter assay were used in our study, and the 5-year overall survival rate and relapse-free survival rate in different groups were investigated. Results: We found that patients suffering from Helicobacter pylori (Hp) infection were the susceptible population compared to controls. SNP rs56288038 (C/G) in IRF-1 3′UTR was involved in the occurrence of GC by acting as a tumor promoter factor. SNP rs56288038 (C/G) could be up-regulated by miR-502-5p, which caused a down-regulation of IRF-1 in cell lines and decreased apoptosis induced by IFN-γ. Carrying the G genotype was related to significantly low expression of IRF-1 and Hp infection, poor differentiation, big tumor size, invasion depth, as well as the high probability of metastasis, and moreover, the C/G SNP was associated with shorter survival of GC patients with five years of follow-up study. Conclusions: our findings have shown that the SNP rs56288038 (C/G) in IRF-1 3′UTR acted as a promotion factor in GC development through enhancing the regulatory role of miR-502-5p in IRF-1 expression.

A Plackett–Burman design was employed to investigate the effect of various parameters on the denitrification effect of high salinity wastewater treated by biofortification SBR.The purposes of this study were to investigate the significance of pH, DO, C/N, the initial ammonia concentration, salinity, the amount of bacteria and sludge concentration (MLSS).Factors which have significant influence on the denitrification effect of biofortification SBR treatment are quickly screened and used to establish a mathematical model.The results of quick screening provide critical factors for the optimization of operating parameters.

This research adopts the important iron electrolysis, removal of the wastewater of high COD, SS, phosphorus pretreatment experiment. Discusses the different iron dosing quantity, iron carbon ratio, and pH value, reaction time on the COD and turbidity, the influence of the phosphorus removal effect. Studies show that iron important electrochemical test in brewery wastewater treatment static optimal processing conditions for: water pH value is 4, 5% dosage of iron filings, 60 min temperature response, COD removal rate was 52.31%. When the iron carbon ratio of 2:1, removing effect is good, can reach 54.53%.

The analytic hierarchy process is a comprehensive evaluation method, which is a combination of qualitative analysis method and quantitative analysis method, it can simplify the complex problems; this article mainly introduces the features and specific algorithm,carry out a comprehensive evaluation concerning about analysis of problems of on line monitoring about liaohe rive basin water pollution source through layers analyzing method, and compare the rankings to provide thinking for the improvement and optimization of the on line monitoring about liaohe rive basin water pollution source.

Automatic monitoring indicators system of pollution is the key and foundation to construct network of pollutions in total amount monitoring.Scientific and reasonable indicators system can reflect pollutant situation and influence on water environment.Automatic monitoring indicators in pollution of sources are confined to COD and ammonia nitrogen as well as short of monitoring indicators to character features of pollutional sources.It is necessary to conduct optimization and selection of automatic monitoring indicators.Based on beer manufacturing in demonstration area as the research object,analyze sources of pollution in Qinghe basin producing and sewage situation;adopt screening methods to complete optimization and selection of automatic monitoring indicators.Build the system of automatic monitoring indicators in beer manufacturing successfully to provide theoretical support for constructing network of water pollutant total amount monitoring.

To solve the problem of high-salt ballast wastewater treatment, biological contact oxidation process by combined packing used to simulate the experimental study. When dissolved oxygen is 5 to 6, water temperature is 18 ~ 20 °C, pH is 7~8 and hydraulic retention time is 48h, the Influence of salinity dips and swells process on sludge treatment system and its effects were investigated. The results showed that salinity shock loads on the system less affected COD removal, after 3-5 days short adaptability run, the system became able to restore effluent water stability. The influence of Salinity swells process on AOB, NOB and denitrifying bacteria was greater than a dip .Salinity plunged produced smaller impact on the total nitrogen removal, while in the salinity swells TN removal reduced from 70.7% to 42%.

We used a simple low-temperature hydrothermal approach to synthesize Ag nanoparticles (NPs) and demonstrated their efficiency as organic molecule detectors in surface enhanced Raman Scattering (SERS). Using finite difference time domain simulation, we described an investigation on the distribution of electric fields amplitude of the neighboring Ag NPs. The enhanced electric field is confined at the interparticle gaps and the enhancement factor can be further increased with reducing the spacing between the NPs. The theoretical simulation demonstrated good consistency with the experimental measurement results, which predicts an electric fields amplitude enhancement of 115 at the center of NPs gap and an electromagnetic SERS enhancement of 108. The evidence of clear correlations between SERS enhancement and morphology distribution offer a route to develop more effective SERS substrates.

Six dominant thermophilic cellulolytic bacteria were separated from undigested dewatered sludge. By analysis of their water content, value of hydrolysis up, rate of weight loss of filter paper and CMC-enzyme activity, a dominant thermophilic cellulolytic bacterium named XW5was separated and inoculated in the dewatered sludge under simulating bio-drying conditions. The results showed that water content of the dewatered sludge which was inoculated dominant bacterium was decreased from an initial 70.05% to 62.49%.

The numbers of index of Chinese standards for drinking water quality increased from 17 by the Health Regulation for Drinking water promulgated 1959 to106 by the current Standards for Drinking Water Quality (GB5749-2006). The Standard has been developed at the same pace with the world. However, there were some hysteretic problems in the process of standards implementation due to the detection technique. The article was aimed at making suggestions on improving detection capability and testing equipment, making programs on the technical personnel and the regional differences. The present research results will lay the foundation for the implementation of Chinese standards for drinking water quality.

In order to improve beer sterilization effect of wastewater treatment, reduce the heat load of the sterilization wastewater on subsequent water treatment processes. In this paper, through optimizing transformation developed micro-flocculation -membrane treatment processes. Screening for the identification of inorganic polymeric aluminum ferric chloride flocculants as the technology. The best dosage was 15mg / L, the field test to verify the craft beer sterilized wastewater treatment effect. The results show that, micro flocculation membrane treatment process in the water after treatment of COD and turbidity removal rate is respectively 76% and 98%. To illustrate the use of this technology in the treatment of beer sterilizing water without two stage filtration, can effectively recycling sterilized wastewater.

Through running anaerobic ammonia oxidation reactors for a few days, we found that inorganic carbon source has great influence on anaerobic ammonia oxidation reactions. The inorganic carbon source increasing, the effect of anaerobic ammonia oxidation bacteria reaction is much better and the processing rate is high. And if there are no inorganic carbon source, anaerobic ammonia oxidation reactions are running anomaly. The processing rate of nitrate nitrogen is about 80%, and the processing rate of ammonia nitrogen is only 30% or so. The anaerobic ammonia oxidation bacteria metabolic is getting worse and worse.

The paper introduced the source and harmfulness of heavy metal pollution in water environment and summarized the applications of the chemical method, physiochemical method and biological method in heavy metal pollution of sudden emergency accident. Meanwhile, the heavy metal pollution emergency processing technology of rivers, lakes and other water bodies were elaborated and the emergency engineering technology of heavy metal pollution in rivers and lakes were analyzed. Finally, the application of bioremediation on heavy metal pollution caused by sudden emergency accident was prospected.

The treatment efficiency of Da Huofang Reservoir water in low temperature and low turbidity period by Coagulation/Ultrafiltration process was investigated in pilot study, and the water quality treated by the process was compared with that by the traditional treatment process. When coagulant dosage is 30 mg/L, the average removal rates of turbidity, CODMn and UV254 were 89.2%, 47.3% and 52.8% respectively in the coagulation/ultrafiltration process, and the treatment efficiency was obviously superior to the traditional process. In the study of operation parameters optimization for Coagulation/Ultrafiltration process, the optimum membrane flux was determined that was 50 L/m2h. The cleaning membrane water was treated by coagulation sedimentation process, and the quality of effluent was good which was mixed with raw water for reuse, and the experiment indicated that this method did not affect the stable operation of Coagulation/Ultrafiltration process, eventually the water recycling rate was improved from 89% to 96.5%.

This article describes the simulation method of water temperature and the model classification of water quality in lakes and reservoirs, and classic model of ecosystem dynamics of lakes and reservoirs - BA model from the water quality parameters of the relationship between two aspects of the basic equations are introduced. On this basis, establish the eutrophication of ecological models of lakes and reservoirs.

Under the unique long-time low temperature conditions (average 8°C) of the northern winter in China, the effects of temperature on water quality and the performance of Powder activated carbon-I Membrane biological reactor (PAC-IMBR) were studied and demonstrated quantitatively by the NH4+-N value, chemical oxygen demand (CODCr) and transmembrane pressure (TMP). The results showed that the supernatant NH4+-N decrease from 1.20 mg·L-1 to 0.81 mg·L-1 with the temperature rising from 8°C to 20°C, while 30.8 mg·L-1 to 23.1 mg·L-1 for the CODCr. However, the temperature rising have no marked influence on the effluent NH4+-N and CODCr due to the filtration of membrane. TMP was measured to characterize membrane fouling and it is clear that the TMP and its increasing rate descend with the temperature rising, which demonstrates that the raising temperature is advantageous to prolong the cycle of PAC-IMRB and alleviate the membrane fouling.

This paper studies the impact of cooling and warming on shortcut and simultaneous nitrification and denitrification under low temperature. The results show that the effluent concentration of NH4+-N and TN gradually increased, the nitrite accumulation rate decreased when DO was 0.3~0.5 mg•L-1, sludge return ratio was 300%, PH was 7.5~8.5, temperature dropped from 15 °Cto 8°C. From 10°C to 8°C in cooling, the average nitrite accumulation rate was 58.17% in A (HRT=24h) reactor. During the system heating process, treatment effect of the system gradually changed for the better. From 12°C to 15°Cin heating process, the average nitrite accumulation rate was 74.39% in B (HRT=48h) reactor. The system treatment effect in B reactor was better by contrasting A and B reactors, therefore, we can increase HRT to reduce the adverse effects on the system due to the temperature decreases to enhance TN removal effect of system.

Bio-drying reduces the moisture content of sludge without other external energy consumption, this paper introduced the microbiological aspect of a bio-drying process ,and state briefly about the suitable scope of oxygen supply ,C/N ratio, pH of biomass and moisture content for a optimal heat generation.

Complex microbial inoculants are highly effective compound bacteria which are widely used in the removal of pollutants, such as organic matter, nitrogen and phosphorus in the paper, printing and dyeing, petroleum and high-salt wastewater. The development of complex microbial inoculants was introduced in this paper, especially their application and influence factors in wastewater treatment. Finally we put forward current problems and development prospects of complex microbial inoculants.

To construct a co-culture system for bone marrow mesenchymal stem cells (BMMSC) and multiple myeloma (MM) cells, and to investigate the effects of co-cultured BMMSC on the migrating and homing of multiple myeloma cells.The BMMSC from the transgenic mice with green fluorescent protein (GFP) fetal bone were cultured by adherent screening. A co-culture system of BMMSC and MM cell line XG-7 cells was constracted, the proliferation and apoptosis of cells were determined by trypan blue exclusion and Annexin V/PI, respectively, MDC staining was employed to detect the autophagy. The moving direction distribution of molecule in BMMSC and XG-7 cells labeled with PE-CD138 in co-culture process were observed dinamically by confocal microscopy.After co-culture with GFP-BMMSC, the resistance of XG-7 cells to apoptosis and autophagy were enhanced; at the same time, their proliferation increased. Apoptosis rates of XG-7 cells directly and indirectly co-cultured with BMMSC were (6.23 ± 0.12)% and (6.97 ± 0.03)% respectively, which were lower than that of XG-7 cells cultured alone (17.90 ± 1.46)% (P0. 01). There was low level of autophagy in XG-7 cells co-cultured with BMMSC. XG-7 cells are highly polarized and contained a specialized membrane domain with specific protein and lipid components to contact with BMMSC under confocal microscope. After methyl-β-cyclodextrin treatment, the molecules were normally enriched in the specialized domain.BMMSC can protect XG-7 cells from apoptosis and autophagy, and obviously promote the proliferation of XG-7 cells, and can influence the migrating and homing of multiple myeloma cells.

The invasion of indigenous flora from the open system into the biological enhanced activated carbon(BEAC) system can inhibit the development of dominant bacteria and also decrease the biodegradability and biological activity of dominant bacteria. Therefore, this study aimed to investigate the effect of backwashing on the indigenous flora and dominant bacteria in the BEAC system and to study the optimal operation process of backwashing. In order to control the inhibition of the indigenous flora, the optimal backwashing conditions were set as 7-10d of operation period and 8-10 L/(m2•s) of air backwashing intensity. In addition, the PCR-DGGE results showed the indigenous flora could be removed under the optimal backwashing process, and the dominant bacteria could also be updated to maintain the biological stability well in this system.

China has the largest population in the world, and the available water resources per capita are very limited. The rapid economic growth and urbanization has caused increased water demand in China’s urban areas. Urban water quota management has become a core strategy for establishing a water-saving society. In 2001-2008, the trend of total water consumption has changed obvious in China. And the water consumption of public service has the same trend. Whit the development of the third industry and the increase of use efficiency, in the future the trend of public service water consumption will maintain stable situation. It still need further improve the water management to control the water increasing. The comprehensive water quota as the standard can estimate the water-saving level of the industries. And it can also determine whether the industry meet the water-saving requirements. Compared with the part quota, it simplifies the process of collecting the water data and improving the quota management operation, which avoid the false data. Researching the comprehensive water quota is a critical part of water quota management.

The experiment uses the Shenyang northern sewage treatment plant two sink the pond sludge was the indigenous Thiobacillus enrichment culture, through the matrix of different proportion, to find the most suitable matrix proportion, so as to shorten the bioleaching process start time, improve the bioleaching process for heavy metal removal efficiency. By usihng the method developed by mixing S bacteria predominantly mixed bacteria liquid, will raise the mixed strain cycle was shortened to 11 days, the best ratio of S, Fe2+ matrix is determined as 3 : 1, the matrix culture mixed Thiobacillus is faster, and can greatly improve the bioleaching boot efficiency. Fe2+ dosing is able to activate the bacterial Fe2+ enzyme synthesis, be helpful for accelerating thiobacilli on s oxidation using elemental sulfur, while also promoting microorganism to use Fe2+. Using the technology of biological leaching experiment on heavy metal removal rate is higher, compared with a single strain has more engineering application and popularization value.

The passage makes an analysis about water utilization structure by consulting literature and questionnaire survey. Household water consumption rose steadily and its proportion in domestic water rose from 39.8% in 1999 to 66.8% in 2008, and the flushing and bathing accounted for the largest amount of household water, its proportions is 27.1% and 26.4%. The bathing water of the southern city was wasted; it produces water-using potential. So the prevalence rate of the advanced instruments is important for using water efficiently.

With nitrite or nitrate nitrogen as electron acceptor in the high salt conditons, halophilic denitrifying bacteria can transfer nitrite or nitrate to nitrogen, thereby purifying the high-salt wastewater. Halophilic denitrifying bacteria play an important role in the carbon and nitrogen removal of saline wastewater, such as petroleum, chemical industry, seafood processing and seafood farming. This article dissussed halophilic denitrifying bacteria screening, the main types and the corresponding morphological characteristics, then we focused on the research progress of main factors of halophilic denitrifying bacteria’s growth and nitrogen removal. Finally put forward the current problems of the research and development trend of halophilic denitrifying bacteria.

Simultaneous powdered activated carbon(PAC) adsorption and dominant bacteria biodegradation process was evaluated for the aniline pollution accident existing in source water. The jar test results showed the aniline adsorption capacity and adsorption rate of the PAC were represented by the Freundich adsorption isotherm model. The aniline adsorption ability of the PAC was high when the temperature was low; the biological activity of dominant bacteria was high when the temperature was at 25°C and pH value was at 6. The poly-aluminum-chloride in combination with PAC and potassium permanganate in combination with PAC were compared with the simultaneous adsorption and biodegradation.

Two different kinds of water were treated with aeration-contact oxidation filtration process. One was groundwater containing iron and manganese (GIM), the other was GIM polluted by ammonia (GAIM). The results showed that ammonia has almost no influence on iron removal, but has great influence on manganese. Under the condition of 1m/h filter velocity and 10 mg/L average ammonia nitrogen concentration of inlet，the outlet manganese concentration for GAIM was less than 0.1 mg/L after 315 days. When temperature dropped to 18°C below, the manganese concentration of effluent exceeded 0.1 mg/L. When average ammonia nitrogen concentration of inlet for GAIM dropped to 3.27 mg/L, the manganese concentration of outlet for GAIM was less than 0.1 mg/L in winter. And for GIM, the manganese concentration of outlet was less than 0.1 mg/L only after 52 days. Although the filter velocity increased gradually from 1 to 7 m/h, the manganese concentration of effluent was always less than 0.1 mg/L. Therefore, ammonia pollution in the groundwater greatly increases the difficulty of water treatment.

The current situations of water environmental problem, water quality, water pollution prevention and control of the Liao River are analyzed and evaluated in this paper. The results show that a serious current situation of the Liao River water quality. The main pollution sources are: grave mountain forest vegetation destruction; serious soil and water loss; densely populated city; great pressure of sewage treatment; low coverage rate of the urban sewage pipe network; serious phenomenon for sewage straightly discharge and level of recycled water reuse. This paper aims at putting forward more attention on the work of basin pollution prevention and treatment; fundamentally relieve water source-oriented supply pressure.

The effects of temperature on electricity performance and microbial activity were investigated in single-chamber microbial fuel cell with proton exchange membrane (S-PEM-MFC) using glucose as substrate with phosphate buffer solution(PBS). The results showed that S-PEM-MFC able to adapt to a wide temperature range of 11, 18, 25, 30 and 35°C. The open circuit voltage, polarization, power density and microbial activity of S-PEM-MFC were increased with increasing temperature from 11 to 30°C. The maximum power density were 193.8mW∙m-3 at 30°C. Compared to 30°C, the battery open circuit voltage increased by only 4.8% at 35°C, while the polarization and power density is almost the same. These results demonstrate that according to the principle of economy which 30°C should be the optimal operating temperature of S-PEM-MFC.

One superior iron and manganese bacteria were separated from the stable operation of porcelain granular BAF filters of removing iron, manganese and ammonia. The bacteria was domesticated at low temperature. By analyzing the sample water containing iron and manganese in the role of iron and manganese bacteria which was not domesticated and domesticated at different temperature, observing the Iron and manganese concentration with time going on, studying the bacteria’s removal of iron and manganese property and the domesticated effect. Studies show that: the selected bacteria with 1% bacterial liquid at proper temperature within 48h ,the removal rate of iron and manganese was 75% and 35% respectively;After domesticated at low temperature, the removal rate of the iron and manganese domesticated bacteria at 10°C was improved 0.4 and 2 times more than the before domesticated; The iron and manganese domesticated bacteria at 10°C did not grow at 4°C,but the bacteria’s removal rate was better than the bacteria cultured at 30°C,and the iron removal rate was improved from 23% to 35%,the manganese removal rate was improved from 5% to 11%.

Biological aerated filter (BAF) was employed to remove iron, manganese and ammonia from groundwater. The filter was in continuous operation under hydraulic loading of 3m3/m2.h. The influence of air-water ratio, the removal effects of iron, manganese and ammonia respective, and the oxidation of the three pollutants, the change of dissolved oxygen along the filter depth were investigated in this research. And the results was obtained, that the optimal air-water ratio was determined by 3, which made the dissolved oxygen concentration in the filter central be 4.75mg/l, and the removal of iron, manganese and ammonia be above 90%, 99% and 87%, respectively. The efficient removal of manganese occurred in the central and lower parts of the filter. More iron was oxidated at the upper 0.75m of the filter, and the oxidation velocity of manganese and ammonia would be limited, when the inlet iron concentration was higher. Nitrification process consumed more dissolved oxygen, which made dissolved oxygen concentration decrease along the filter depth from 0 to 1.25m. The demand of filtration depth is 1.6m, for simultaneous removal.

Six dominant microorganisms including four kinds of Pseudomonas and two kinds of Bacillus were isolated from substrate sludge for improving the organic substance removal ability for the biological activated carbon filter (BAC). This paper examines the backwashing effect on the stabilization of these six microorganisms immobilized on the filter. The results showed the backwashing had more impact on bacteria stabilization of BAC filter during start-up period than the stabilization period. The biomass loss was 28.57% during the start-up period more than the stabilization period which biomass loss was 11.76%. The Scan Electron Microscope (SEM) and Denaturing Gradient Gel Electrophoresis of Polymerase Chain Reaction amplified 16S rRNA (PCR-DGGE) photos showed that the ecology of the population did not change with the operation. The biomass of dominant microorganism after air-water scour backwashing decreased during the start-up period and retained stability during long term performance.

Electricity can be generated during the wastewater treatment by microbial fuel cell (MFC). Effect of the anode pretreatment method, anode modification and electronic media in anolyte on electricity generation of a single chamber microbial fuel cell (SCMFC) with an air cathode was investigated by performance measurement of the MFC during one cycle of generating electricity. Results show that the discharge time of MFCs with stainless steel anode pretreated by NH4Cl and HCl is longer than that with anode pretreated by the distilled water and NaOH; the performance of MFC with anode pretreated by NH4Cl is the best; comparison with stainless steel anode and adhere activated carbon, the current of MFC with the stainless steel anode adhere the carbon fiber is the maximum and its Coulombic efficiency is the highest; The electricity generation characteristics of the MFC with the anode adhered carbon fiber and pretreated by NH4Cl and the anolyte added the electronic media is the best. The action of the electronic medium and the NH4Cl on the MFC is synergism. It's of great significance that the research results improve the performance of MFC by anode pretreatment.

The filter media from iron and manganese biological removal filter which w- as replaced by new termly can be re-used. The sand filter media was characterized u- sing the SEM and owns developed interspaces. Drying the sand filter media at 110°C, then inspect and analyse its character and mode of defluoridation。The results show as follows. Under the test condition ,the adsorption balance can come out in about 3 hou-rs , the fluoride sorption on the re-used matured filter media followed pseudo-second kinetics. The effect of defluoridation by the matured filter media will be better within the range of pH 3~7, and best at about pH 4.58. The primary mode of fluoride sorpti- on is chemism, but physical sorption is also in existence , and the adsorption isother- ms could be described by Langmuir equation. Contact-time is a vary important factor in the continuous-flow column experiment. The effect of fluoride removal is long，as the height of filter column is controlled as 40cm.The filter media from iron and manga- nese biological removal filter possess potential of perfect sorbent, space for deeply study and foreground of practical application.

The inoculation of short-cut denitrifying polyphosphate-accumulating organisms (DPAOs) mainly included two-phase inoculation and three-phase inoculation. The short-cut denitrifying phosphorus removal bacteria were quickly inoculated by sequencing batch reactor (SBR) to treatment domestic wastewater. The results showed that the average effluent concentration of TP was 0.85 mg/L after 132 cycles by 44 days in two-phase inoculation. The removal rates of NO2 --N, TP and COD were 94.73%, 95.47% and 89.96% after 126 cycles by 42 days in three-phase inoculation, and the effluent concentrations were separately 1.31 mg/L, 0.45 mg/L and 17.07 mg/L, which reached the first A class requirement of Urban sewage treatment plant pollutant discharge standard. It was indicated that the efficiency of three-phase inoculation was higher. Anoxic phosphorus uptake was influenced seriously by anaerobic residual carbon, and it was the difference of the two inoculations.

To investigate the sensitivity to bortezomib of RPMI8226 cells after co-cultured with down-regulated Caveolin (Cav)-1 expression of HUVECs by transfection with Cav-1 shRNA (HUVECs(Cav-1 low)).Exposure to bortezomib with or without 50 nmol/L dexamethasone at different concentration, the proliferation of RPMI8226 was analyzed by MTT assay when it was cultured alone or co-cultured with HUVECs(Cav-1 low). Cav-1 expression was detected by using of Western blot and cell cycle, apoptosis and the level of reactive oxygen species (ROS) were analyzed by flow cytometry.Cav-1 expression was notably down-regulated in HUVECs(Cav-1 low) (0.2199±0.0288 vs 1.3195±0.2393) (P0.01). The IC(50) of bortezomib for RPMI8226 cultured alone, co-cultured with HUVECs orHUVECCav- 1 low were 20 nmol/L, 50 nmol/L and 65 nmol/L, respectively. The percentages of G₀/G₁ phase in RPMI8226 cultured alone, co-cultured with HUVECs and HUVECs(Cav-1 low) were 28.49%, 30.41%, and 36.15% respectively. The protection of RPMI 8226 against apoptosis by HUVECs was demonstrated that the apoptosis/death rates were 66.8%, 10.7% and 8.6% in RPMI8226 cultured alone, co-cultured with HUVECs and HUVECs(Cav-1 low) after exposure to 20 nmol/L bortezomib for 24 h. RPMI8226 could induce the oxidative stress of HUVECs before and after co-culture. The ROS level was raised from 15.0% to 35.2% in RPMI8226, from 80.4% to 91.0% in HUVECs, and from 84.6% to 96.8% in HUVECs(Cav-1 low).The down-regulated Cav-1 expression of HUVECs could promote proliferation and induce apoptosis of RMPI8226 cells, lead to G₀/G₁ phase arrest, and reduce the sensitivity to bortezomib.

To construct a co- culture system of mesenchymal stem cells (MSC) and multiple myeloma (MM) cells and investigate the alterations of connexin 43 (CX43) expression and stromal derived growth factor (SDF)- 1α secretion of MSC.CX43 expression and SDF- 1α secretion of MM cell lines (RPMI8226) and human primary MM cells were analyzed by western blot and immunofluorescence. Western blot, RT- PCR and immunofluorescence were employed to detect the alterations of CX43 expression and distribution in MSC directly and indirectly co-cultured with myeloma cells. Lucifer yellow dye spread was utilized to evaluate gap junctional intercellular communication (GJIC) between co- cultured MSC. Transwell was applied to study the transmigration of RPMI8266 induced by MSC under the condition of 18α- glycyrrhetinic acid (18α-GA). The level of SDF- 1α was detected by EILSA.RPMI8266, U266 and one-third primary MM cells expressed CX43 at low or moderate levels. CX43 wasn't expressed in XG- 4 and XG- 7 cells but highly expressed in MSC. The expressions of CX43 mRNA of MSC were up- regulated after directly and indirectly co- cultured with RPMI8226, 1.36 and 2.10 times that of MSC cultured alone respectively. Western blot analysis showed that CX43 protein expression of MSC was also up-regulated, mainly distributed in cytoplasm. Lucifer yellow dye spread showed that GJIC was up-regulated in MSC. SDF-1α concentration in supernatant of MSC directly and indirectly co-cultured with RPMI8226 were (373.02±10.11)pg/ml and (309.71±10.71)pg/ml respectively, which were higher than that of MSC cultured alone (237.84±9.23)pg/ml (P0.01), and could be inhibited by 18α-GA [(237.84±9.23)pg/ml and (94.31±6.44)pg/ml] respectively (P0.01). 18α-GA could inhibit the transmigration of RPMI8226 induced by MSC, decrease from (8.00±0.67)% to (4.82±0.19)%.CX43 expression of MSC was up-regulated after directly and indirectly co-cultured with MM cells, which could improve the level of SDF-1α secretion of MSC. GJ inhibitor could downregulate SDF-1α secretion of MSC and inhibit the transmigration of MM cells induced by MSC.

To investigate the influence of autophagy on the survival and proliferation of multiple myeloma (MM) cells.Multiple myeloma (MM) cell line U266 cell autophagy was induced by serum-free culture condition, and adding rapamycin or 3-MA respectively. The cells proliferation was observed. U266 cells, lymphoma cell Jurket under normal culture condition, and serum-free cultured Jurket cell were used as control group. The proliferation and apoptosis of cells were determined by CCK8 and flow cytometry, respectively. MDC staining were employed to detect the autophagy. The mRNA expression of Mtor and Beclin1 gene of U266 cells were assayed by RT-PCR. Protein LC3I/LCII and LAMP1 was analyzed by western blot.There was low level of autophagy in U266 cells, sera starvation increased the level of autophagy. Rapamycin upregulated autophagy of the U266 cells and stimulated their proliferation. But the autophagy level of sera starvation and rapamycin group declined when culture for 96h.3-MA had the same effects on U266 cells, although it was on 24 h. But rapamycin and 3-MA could inhibit cell proliferation under normal culture condition. Compared with normal culture condition, apoptosis of U266 cells increased significantly after 24h incubation in medium without sera \[(1.33 ± 0.09)% and (17.90 ± 1.46)%, respectively\] (P0.01). Rapamycin and 3-MA could inhibit the serum-free induced apoptosis \[(6.23 ± 0.12)% and (6.97 ± 0.03)%, respectively\](P0.01), but cell apoptosis was at the same level after 72 hour incubation \[(30.37 ± 0.27)%, (30.13 ± 1.93)% and (28.57 ± 2.83)%, respectively\] (P0.05). However, apoptosis of U266 cells decreased to 18.7% and 12.6% after removal of rapamycin and 3-MA.There is basically level of autophagy in MM cells which is higher than those in the Jurkat cells. Both Rapamycin and 3-MA can inhibit the cells proliferation under normal culture condition. Up-regulated autophagy promotes survival and proliferation of MM cells under sera deletion. Rapamycin strengthens this effect with limited duration. 3-MA has dual effects on cell autophagy.

To observe the effect of rosiglitazone (RGZ) and all-trans-retinoic acid (ATRA) on the growth of myeloma xenograft in nude mice and to explore the influence of RGZ and ATRA on VEGF expression and angiogenesis in the tumor.VEGF gene expression in myeloma cell line U266 cells was analyzed by semi-quantitative RT-PCR after incubation with RGZ, ATRA, or RGZ + ATRA for 24 h. Myeloma xenograft was established by subcutaneous injection of 10(7) U266 cells in the scapula area of 4-week old nude mice. 7 days later, the nude mice were administered with RGZ, ATRA or RGZ + ATRA, respectively, by intraperitoneal injection once every day for 21 days. The control mice were given equal volume of normal saline instead of the drug. On the 21(st) day of treatment, the mice were sacrificed and the tumors were taken off, and the tumor volume and weight were measured. The tumors were examined by histopathology with HE staining, and microvessel density (MVD), CD34 and VEGF expression in the tumors were analyzed by immunohistochemical staining.VEGF mRNA was highly expressed in U266 cells and was decreased in a dose-dependent manner after incubation with RGZ. The VEGF mRNA level was further more decreased after RGZ + ATRA treatment. Xenografts of U266 cells were developed in all nude mice. The volume and weight of xenografts in the RGZ group were (785 ± 262) mm(3) and (1748 ± 365) mg, respectively, significantly lower than those of the control group (both P0.01). More significant inhibition was in the RGZ + ATRA group, (154 ± 89) mm(3) and (626 ± 102) mg, respectively, both were P0.05 vs. the RGZ group. RGZ inhibited the angiogenesis in U266 xenografts and immunohistochemical staining showed that the tumor MVD and VEGF expression were significantly decreased by RGZ treatment, and further more inhibited in the RGZ + ATRA group. VEGF protein was expressed in all xenografts in the nude mice. Its immunohistochemical staining intensity was 2.20 ± 0.40 in the control group, significantly higher than that of 1.48 ± 0.37 in the RGZ group (P0.01), and that of RGZ + ATRA group was 0.58 ± 0.26, further significantly lower than that of the RGZ group (P0.01). CD34 was expressed in all xenografts, most highly in the control group and lowest in the RGZ + ATRA group. The microvessel density (MVD) was highest in the control group (56.4 ± 15.2), significantly lower in the RGZ group (44.6 ± 11.2) (P0.05), and lowest in the RGZ + ATRA group (21.5 ± 8.6, P0.01).The growth of myeloma cells can also be inhibited by RGZ and ATRA in nude mice in vivo. In addition to differentiation and apoptosis induction, RGZ can inhibit the formation of myeloma xenograft probably also through the downregulation of VEGF expression and subsequent angiogenesis.

Ferric nitrate/activated carbon powder catalyst was obtained through impregnation and Fe/C catalyst was adsorbed on carbon felt as air cathode electrodes. Effects of activated carbon powder dosage and ferric nitrate concentration on electricity generation of MFC with landfill leachate as fuel were measured. Performances of cathodes obtained at different ferric nitrate concentrations were evaluated by cyclic voltammetry tests. The results showed that with the increase of activated carbon powder dosage or the iron nitrate concentration, MFC produce electrical properties showed a decreasing trend after the first rise. When the activated carbon powder dosage was 1 g and the iron nitrate concentration was 0.25 mol x L(-1), it was proved to be an optimum cell performance for 4199.8 mW x m(-3) output power and 465 omega apparent resistance. Under the optimal ratio rang between ferric nitrate and activated carbon powder, MFC apparent resistance decreased and the power density increased respectively with the increase of catalyst total dosage. The best produce electrical properties of MFC with Fe/C catalyst for 0.25 mol x L(-1) iron nitrate and 1 g activated carbon powder dosage was observed by cyclic voltammetry tests. The output power of MFC and the removal quantity increased with the concentration of inlet and the maximum values were respectively 5478.92 mW x m(-3) and 1505.2 mg x L(-1). the maximum removal rates of COD achieved at 89.1%.