381 results on '"Jin JS"'
Search Results
2. Parallel rough set: Dimensionality reduction and feature discovery of multi-dimensional data in visualization
- Author
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Huang, TH, Huang, ML, and Jin, JS
- Subjects
Artificial Intelligence & Image Processing - Abstract
Attempt to visualize high dimensional datasets typically encounter over plotting and decline in visual comprehension that makes the knowledge discovery and feature subset analysis difficult. Hence, reshaping the datasets using dimensionality reduction technique is paramount by removing the superfluous attributes to improve visual analytics. In this work, we applied rough set theory as dimensionality reduction and feature selection methods on visualization to facilitate knowledge discovery of multi-dimensional datasets. We provided the case study using real datasets and comparison against other methods to demonstrate the effectiveness of our approach. © 2011 Springer-Verlag.
- Published
- 2011
3. Automated detection of amnestic mild cognitive impairment in community-dwelling elderly adults: A combined spatial atrophy and white matter alteration approach
- Author
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Cui, Y, Wen, W, Lipnicki, DM, Beg, MF, Jin, JS, Luo, S, Zhu, W, Kochan, NA, Reppermund, S, Zhuang, L, Raamana, PR, Liu, T, Trollor, JN, Wang, L, Brodaty, H, and Sachdev, PS
- Subjects
Aged, 80 and over ,Male ,Neurology & Neurosurgery ,Brain ,Reproducibility of Results ,Image Enhancement ,Nerve Fibers, Myelinated ,Sensitivity and Specificity ,Pattern Recognition, Automated ,Diffusion Tensor Imaging ,Image Interpretation, Computer-Assisted ,Humans ,Female ,Amnesia ,Atrophy ,Cognition Disorders ,Geriatric Assessment ,Aged - Abstract
Amnestic mild cognitive impairment (aMCI) is a syndrome widely considered to be prodromal Alzheimer's disease. Accurate diagnosis of aMCI would enable earlier treatment, and could thus help minimize the prevalence of Alzheimer's disease. The aim of the present study was to evaluate a magnetic resonance imaging-based automated classification schema for identifying aMCI. This was carried out in a sample of community-dwelling adults aged 70-90. years old: 79 with a clinical diagnosis of aMCI and 204 who were cognitively normal. Our schema was novel in using measures of both spatial atrophy, derived from T1-weighted images, and white matter alterations, assessed with diffusion tensor imaging (DTI) tract-based spatial statistics (TBSS). Subcortical volumetric features were extracted using a FreeSurfer-initialized Large Deformation Diffeomorphic Metric Mapping (FS. +. LDDMM) segmentation approach, and fractional anisotropy (FA) values obtained for white matter regions of interest. Features were ranked by their ability to discriminate between aMCI and normal cognition, and a support vector machine (SVM) selected an optimal feature subset that was used to train SVM classifiers. As evaluated via 10-fold cross-validation, the classification performance characteristics achieved by our schema were: accuracy, 71.09%; sensitivity, 51.96%; specificity, 78.40%; and area under the curve, 0.7003. Additionally, we identified numerous socio-demographic, lifestyle, health and other factors potentially implicated in the misclassification of individuals by our schema and those previously used by others. Given its high level of performance, our classification schema could facilitate the early detection of aMCI in community-dwelling elderly adults. © 2011 Elsevier Inc.
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- 2011
4. A three-level framework for affective content analysis and its case studies
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Xu, M, Wang, J, He, X, Jin, JS, Luo, S, Lu, H, Xu, M, Wang, J, He, X, Jin, JS, Luo, S, and Lu, H
- Abstract
Emotional factors directly reflect audiences' attention, evaluation and memory. Recently, video affective content analysis attracts more and more research efforts. Most of the existing methods map low-level affective features directly to emotions by applying machine learning. Compared to human perception process, there is actually a gap between low-level features and high-level human perception of emotion. In order to bridge the gap, we propose a three-level affective content analysis framework by introducing mid-level representation to indicate dialog, audio emotional events (e.g., horror sounds and laughters) and textual concepts (e.g., informative keywords). Mid-level representation is obtained from machine learning on low-level features and used to infer high-level affective content. We further apply the proposed framework and focus on a number of case studies. Audio emotional event, dialog and subtitle are studied to assist affective content detection in different video domains/genres. Multiple modalities are considered for affective analysis, since different modality has its own merit to evoke emotions. Experimental results shows the proposed framework is effective and efficient for affective content analysis. Audio emotional event, dialog and subtitle are promising mid-level representations. © 2012 Springer Science+Business Media, LLC.
- Published
- 2014
5. Camera control and multimedia interaction using individual object recognition
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Xu, RYD and Jin, JS
- Abstract
Currently, most of the automated, computervision assisted camera control policies are based on human events, such as the speaker gesture and position changes. In addition to these events, in this paper, we introduce a set of natural camera control and multimedia synchronization schemes based on the individual object interaction. We describe in detail, how our unique method, in which the head-pose estimation are used to compute the region of interest (ROI) for recognizing the hand-held object. We explain, from our results, how our approach has achieved robustness, efficiency and unambiguous object interaction during real-time video shooting. © 2007 ACADEMY PUBLISHER.
- Published
- 2007
6. Latency insensitive task scheduling for real-time video processing and streaming
- Author
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Xu, RYD and Jin, JS
- Subjects
Artificial Intelligence & Image Processing - Abstract
In recent times, computer vision and pattern recognition (CVPR) technologies made automatic feature extraction, events detection possible in real-lime, on-the-fly video processing and streaming systems. However, these multiple and computational expensive video processing tasks require specialized processors to ensure higher frame rate output. We propose a framework for achieving high video frame rate using a single processor high-end PC while multiple, computational video tasks such as background subtraction, object tracking, recognition and facial localization have been performed simultaneously. We show the framework in detail, illustrating our unique scheduler using latency insensitive tasks distribution and the execution coulent parameters generation function (PGF). The experiments have indicated successful results using high-end consumer type PC. © Springer-Verlag Berlin Heidelberg 2005.
- Published
- 2005
7. Vehicle type classification using data mining techniques
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Peng, Y, Jin, JS, Luo, S, Xu, M, Au, S, Zhang, Z, Cui, Y, Peng, Y, Jin, JS, Luo, S, Xu, M, Au, S, Zhang, Z, and Cui, Y
- Abstract
© 2013 Springer Science+Business Media, LLC. All rights reserved. In this paper, we proposed a novel and accurate visual-based vehicle type classification system. The system builts up a classifier through applying Support Vector Machine with various features of vehicle image. We made three contributions here: first, we originally incorporated color of license plate in the classification system. Moreover, the vehicle front was measured accurately based on license plate localization and background-subtraction technique. Finally, type probabilities for every vehicle image were derived from eigenvectors rather than deciding vehicle type directly. Instead of calculating eigenvectors from the whole body images of vehicle in existing methods, our eigenvectors are calculated from vehicle front images. These improvements make our system more applicable and accurate. The experiments demonstrated our system performed well with very promising classification rate under different weather or lighting conditions.
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- 2013
8. Hierarchical affective content analysis in arousal and valence dimensions
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Xu, M, Xu, C, He, X, Jin, JS, Luo, S, Rui, Y, Xu, M, Xu, C, He, X, Jin, JS, Luo, S, and Rui, Y
- Abstract
Different from the existing work focusing on emotion type detection, the proposed approach in this paper provides flexibility for users to pick up their favorite affective content by choosing either emotion intensity levels or emotion types. Specifically, we propose a hierarchical structure for movie emotions and analyze emotion intensity and emotion type by using arousal and valence related features hierarchically. Firstly, three emotion intensity levels are detected by using fuzzy c-mean clustering on arousal features. Fuzzy clustering provides a mathematical model to represent vagueness, which is close to human perception. Then, valence related features are used to detect five emotion types. Considering video is continuous time series data and the occurrence of a certain emotion is affected by recent emotional history, conditional random fields (CRFs) are used to capture the context information. Outperforming Hidden Markov Model, CRF relaxes the independence assumption for states required by HMM and avoids bias problem. Experimental results show that CRF-based hierarchical method outperforms the one-step method on emotion type detection. User study shows that majority of the viewers prefer to have option of accessing movie content by emotion intensity levels. Majority of the users are satisfied with the proposed emotion detection. © 2012 Elsevier B.V.
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- 2013
9. Cortical gyrification and sulcal spans in early stage Alzheimer’s disease
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Liu, T, Lipnicki, D, Zhu, W, Tao, D, Zhang, C, Jin, JS, Sachdev, PS, Wen, W, Cui, Y, Liu, T, Lipnicki, D, Zhu, W, Tao, D, Zhang, C, Jin, JS, Sachdev, PS, Wen, W, and Cui, Y
- Published
- 2012
10. Predicting the development of mild cognitive impairment: A new use of pattern recognition
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Cui, Y, Sachdev, PS, Lipnicki, DM, Jin, JS, Luo, S, Zhu, W, Kochan, NA, Reppermund, S, Liu, T, Trollor, JN, Brodaty, H, Wen, W, Cui, Y, Sachdev, PS, Lipnicki, DM, Jin, JS, Luo, S, Zhu, W, Kochan, NA, Reppermund, S, Liu, T, Trollor, JN, Brodaty, H, and Wen, W
- Abstract
While the conversion from mild cognitive impairment to Alzheimer's disease has received much recent attention, the transition from normal cognition to mild cognitive impairment is largely unexplored. The present pattern recognition study addressed this by using neuropsychological test scores and neuroimaging morphological measures to predict the later development of mild cognitive impairment in cognitively normal community-dwelling individuals aged 70-90. years. A feature selection algorithm chose a subset of neuropsychological and FreeSurfer-derived morphometric features that optimally differentiated between individuals who developed mild cognitive impairment and individuals who remained cognitively normal. Support vector machines were used to train classifiers and test prediction performance, which was evaluated via 10-fold cross-validation to reduce variability. Prediction performance was greater when using a combination of neuropsychological scores and morphological measures than when using either of these alone. Results for the combined method were: accuracy 78.51%, sensitivity 73.33%, specificity 79.75%, and an area under the receiver operating characteristic curve of 0.841. Of all the features investigated, memory performance and measures of the prefrontal cortex and parietal lobe were the most discriminative. Our prediction method offers the potential to detect elderly individuals with apparently normal cognition at risk of imminent cognitive decline. Identification at this stage will facilitate the early start of interventions designed to prevent or slow the development of Alzheimer's disease and other dementias. © 2012 Elsevier Inc.
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- 2012
11. Vehicle type classification using PCA with self-clustering
- Author
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Peng, Y, Jin, JS, Luo, S, Xu, M, Cui, Y, Peng, Y, Jin, JS, Luo, S, Xu, M, and Cui, Y
- Abstract
Different conditions, such as occlusions, changes of lighting, shadows and rotations, make vehicle type classification still a challenging task, especially for real-time applications. Most existing methods rely on presumptions on certain conditions, such as lighting conditions and special camera settings. However, these presumptions usually do not work for applications in real world. In this paper, we propose a robust vehicle type classification method based on adaptive multi-class Principal Components Analysis (PCA). We treat car images captured at daytime and night-time separately. Vehicle front is extracted by examining vehicle front width and the location of license plate. Then, after generating eigenvectors to represent extracted vehicle fronts, we propose a PCA method with self-clustering to classify vehicle type. The comparison experiments with the state of art methods and real-time evaluations demonstrate the promising performance of our proposed method. Moreover, as we do not find any public database including sufficient desired images, we built up online our own database including 4924 high-resolution images of vehicle front view for further research on this topic. © 2012 IEEE.
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- 2012
12. VEHICLE TYPE CLASSIFICATION USING PCA WITH SELF-CLUSTERING
- Author
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Zhang, APJ, Peng, Y, Jin, JS, Luo, S, Xu, M, Cui, Y, Zhang, APJ, Peng, Y, Jin, JS, Luo, S, Xu, M, and Cui, Y
- Abstract
Different conditions, such as occlusions, changes of lighting, shadows and rotations, make vehicle type classification still a challenging task, especially for real-time applications. Most existing methods rely on presumptions on certain conditions, such as lighting conditions and special camera settings. However, these presumptions usually do not work for applications in real world. In this paper, we propose a robust vehicle type classification method based on adaptive multi-class Principal Components Analysis (PCA). We treat car images captured at daytime and night-time separately. Vehicle front is extracted by examining vehicle front width and the location of license plate. Then, after generating eigenvectors to represent extracted vehicle fronts, we propose a PCA method with self-clustering to classify vehicle type. The comparison experiments with the state of art methods and real-time evaluations demonstrate the promising performance of our proposed method. Moreover, as we do not find any public database including sufficient desired images, we built up online our own database including 4924 high-resolution images of vehicle front view for further research on this topic
- Published
- 2012
13. Content on demand video adaptation based on MPEG-21 digital item adaptation
- Author
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Xu, M, He, X, Peng, Y, Jin, JS, Luo, S, Chia, LT, Hu, Y, Xu, M, He, X, Peng, Y, Jin, JS, Luo, S, Chia, LT, and Hu, Y
- Abstract
One of the major objectives in multimedia research is to provide pervasive access and personalized use of multimedia information. Pervasive access of video data implies the access of cognitive and affective aspects of video content. Personalized use requires the services satisfy individual user's needs on video content. This article attempts to provide a content-on-demand (CoD) video adaptation solution by considering users' preference on cognitive content and affective content for video media in general, sports video and movies in particular. In this article, CoD video adaptation system is developed to support users' decision in selecting their content of interest and adaptively deliver video source by selecting relevant content and dropping frames while considering network conditions. First, video contents are annotated by the description schemes (DSs) provided by MPEG-7 multimedia description schemes (MDSs). Then, to achieve a generic adaptation solution, the adaptation is developed following MPEG-21 Digital Item Adaptation (DIA) framework. We study the MPEG-21 reference software on XML generation and develop our own system for CoD video adaptation in three steps: (1) the content information is parsed from MPEG-7 annotation XML file together with bitstream to generate generic Bitstream Syntax Description (gBSD); (2) Users' preference, network characteristic and adaptation QoS (AQoS) are considered for making adaptation decision; (3) adaptation engine automatically parses adaptation decisions and gBSD to achieve adaptation. Unlike most existing adaptation work, the system adapts the content of interest in the video stream according to users' preference. We implement the above-mentioned MPEG-7 and MPEG-21 standards and provide a generic video adaptation solution. Adaptation based on gBSD avoids complex video computation. Thirty students from various departments were invited to assess the system and their responses have been positive. © 2012 Xu et al.
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- 2012
14. Accurate pedestrian counting system based on local features
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Peng, Y, Xu, M, Ni, Z, Jin, JS, Luo, S, Peng, Y, Xu, M, Ni, Z, Jin, JS, and Luo, S
- Abstract
Accurate pedestrian counting are challenging in real-world due to occlusions, pedestrians' overlays or camera view sensitive. In this paper, we propose an accurate and robust pedestrian detection and counting system to address these problems. Our proposed method is group-based, where the count of people in a dense moving group is estimated as a whole. Moving groups containing single or several pedestrians are discriminated from other moving objects. Our method utilizes 9 features of each moving group within a video frame to estimate the pedestrian number in each group. Pedestrian counts are optimized by a novel tracking method, which is based on an analysis of moving groups match, split or merge. Comparison experiments with other two current methods on three benchmark surveillance videos show the effectiveness of our proposed method. © 2012 Springer-Verlag.
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- 2012
15. 3D pose estimation of front vehicle towards a better driver assistance system
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Peng, Y, Jin, JS, Luo, S, Xu, M, Cui, Y, Peng, Y, Jin, JS, Luo, S, Xu, M, and Cui, Y
- Abstract
Driver assistance system enhances traffic safety and efficiency. Accurate 3D pose of front vehicle can help driver to make right decisions on road. We propose a novel real-time system to estimate 3D pose of the front vehicle. This system consists of two parallel threads: vehicle rear tracking and mapping. Vehicle rear is firstly identified in the video captured by an on-board camera, after license plate localization and foreground extraction. 3D pose estimation technique is then employed with respect to extracted vehicle rear. Most 3D pose estimation techniques need prior models or a stereo initialization with user cooperation. It is extremely difficult to obtain prior models due to various appearances of vehicle rears. Moreover, it is unsafe to ask for driver's cooperation when vehicle is running. In our system, two initial key frames for stereo algorithm are automatically extracted by vehicle rear detection and tracking. Map points are defined as a collection of point features extracted from vehicle rear with their 3D information. These map points are inferences that relating 2D features detected in following vehicle rears with 3D world. Relative 3D Pose between current vehicle rear and on-board camera is then estimated through mapping that matches map points with current point features. We demonstrate the abilities of our system by augmented reality, which needs accurate and real-time 3D pose estimation. © 2012 IEEE.
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- 2012
16. A cellular automata hybrid quasi-random Monte Carlo simulation for estimating the one-to-all reliability of acyclic multi-state information networks
- Author
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Yeh, WC, Cao, L, Jin, JS, Yeh, WC, Cao, L, and Jin, JS
- Abstract
Many real-world systems (such as cellular telephones and transportation) are acyclic multi-state information networks (AMIN). These networks are composed of multi-state nodes, with different states determined by a set of nodes that receive a signal directly from these multi-state nodes, without satisfying the conservation law. Evaluating the AMIN reliability arises at the design and exploitation stage of many types of technical systems. However, existing analytical methods fail to estimate AMIN reliability in a realistic time frame, even for smaller-sized AMINs. Hence, the main purpose of this article is to present a cellular automata hybrid quasi-Monte Carlo simulation (CA-HMC) by combining cellular automata (CA, to rapidly determine network states), pseudo-random sequences (PRS, to obtain the exibility of the network) and quasi-random sequences (QRS, to improve the accuracy) to obtain a high-quality estimation of AMIN reliability in order to improve the calculation efficiency. We use one benchmark example from well-known algorithms in literature to show the utility and performance of the proposed CA-HMC simulation when evaluating the one-to-all AMIN reliability. © 2012 ISSN 1349-4198.
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- 2012
17. Bag of Features using sparse coding for gender classification
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Peng, Y, Luo, S, Xu, M, Ni, Z, Jin, JS, Wang, J, Zhao, G, Peng, Y, Luo, S, Xu, M, Ni, Z, Jin, JS, Wang, J, and Zhao, G
- Abstract
Gender classification is challenging. Methods for gender classification need to discriminate subtle differences between male and female. Bag-of-Features (BoF) method with sparse coding has been proven very powerful in image classification. In this paper, we apply BoF method for gender classification. We use two sets of images: training images and testing images. All images are represented by a set of Scale-Invariant Feature Transform (SIFT) descriptors. In training stage, using sparse coding, Visual Words Dictionary (VWD) is constructed from SIFT descriptors extracted from training images. In testing, SIFT descriptors of testing images are approximated by visual words in VWD. The choices of approximating visual words determine the classification decision. We apply our method and another two popular methods on public dataset for gender classification. We achieved promising results. Copyright © 2012 ACM.
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- 2012
18. Combining front vehicle detection with 3D pose estimation for a better driver assistance
- Author
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Peng, Y, Xu, M, Ni, Z, Jin, JS, Luo, S, Peng, Y, Xu, M, Ni, Z, Jin, JS, and Luo, S
- Abstract
Driver assistant systems enhance traffic safety and efficiency. The accurate 3D pose of a front vehicle can help a driver to make the right decision on the road. We propose a novel real-time system to estimate the 3D pose of the front vehicle. This system consists of two parallel threads: vehicle rear tracking and mapping. The vehicle rear is first identified in the video captured by an onboard camera, after license plate localization and foreground extraction. The 3D pose estimation technique is then employed with respect to the extracted vehicle rear. Most current 3D pose estimation techniques need prior models or a stereo initialization with user cooperation. It is extremely difficult to obtain prior models due to the varying appearance of vehicles' rears. Moreover, it is unsafe to ask for drivers' cooperation when a vehicle is running. In our system, two initial keyframes for stereo algorithms are automatically extracted by vehicle rear detection and tracking. Map points are defined as a collection of point features extracted from the vehicle's rear with their 3D information. These map points are inferences that relate the 2D features detected in following vehicles' rears with the 3D world. The relative 3D pose of the onboard camera to the front vehicle rear is then estimated through matching the map points with point features detected on the front vehicle rear. We demonstrate the capabilities of our system by testing on real-time and synthesized videos. In order to make the experimental analysis visible, we demonstrated an estimated 3D pose through augmented reality, which needs accurate and real-time 3D pose estimation. © 2011 Peng et al.
- Published
- 2012
19. Automated detection of amnestic mild cognitive impairment in community-dwelling elderly adults: A combined spatial atrophy and white matter alteration approach
- Author
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Cui, Y, Wen, W, Lipnicki, DM, Beg, MF, Jin, JS, Luo, S, Zhu, W, Kochan, NA, Reppermund, S, Zhuang, L, Raamana, PR, Liu, T, Trollor, JN, Wang, L, Brodaty, H, Sachdev, PS, Cui, Y, Wen, W, Lipnicki, DM, Beg, MF, Jin, JS, Luo, S, Zhu, W, Kochan, NA, Reppermund, S, Zhuang, L, Raamana, PR, Liu, T, Trollor, JN, Wang, L, Brodaty, H, and Sachdev, PS
- Abstract
Amnestic mild cognitive impairment (aMCI) is a syndrome widely considered to be prodromal Alzheimer's disease. Accurate diagnosis of aMCI would enable earlier treatment, and could thus help minimize the prevalence of Alzheimer's disease. The aim of the present study was to evaluate a magnetic resonance imaging-based automated classification schema for identifying aMCI. This was carried out in a sample of community-dwelling adults aged 70-90. years old: 79 with a clinical diagnosis of aMCI and 204 who were cognitively normal. Our schema was novel in using measures of both spatial atrophy, derived from T1-weighted images, and white matter alterations, assessed with diffusion tensor imaging (DTI) tract-based spatial statistics (TBSS). Subcortical volumetric features were extracted using a FreeSurfer-initialized Large Deformation Diffeomorphic Metric Mapping (FS. +. LDDMM) segmentation approach, and fractional anisotropy (FA) values obtained for white matter regions of interest. Features were ranked by their ability to discriminate between aMCI and normal cognition, and a support vector machine (SVM) selected an optimal feature subset that was used to train SVM classifiers. As evaluated via 10-fold cross-validation, the classification performance characteristics achieved by our schema were: accuracy, 71.09%; sensitivity, 51.96%; specificity, 78.40%; and area under the curve, 0.7003. Additionally, we identified numerous socio-demographic, lifestyle, health and other factors potentially implicated in the misclassification of individuals by our schema and those previously used by others. Given its high level of performance, our classification schema could facilitate the early detection of aMCI in community-dwelling elderly adults. © 2011 Elsevier Inc.
- Published
- 2012
20. Cascade-based license plate localization with line segment features and haar-like features
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Peng, Y, Xu, M, Jin, JS, Luo, S, Zhao, G, Peng, Y, Xu, M, Jin, JS, Luo, S, and Zhao, G
- Abstract
AdaBoost classifiers with Haar-like features are widely used for license plate (LP) localization. However, it normally requires high-dimensional Haar-like features which cause extremely high computational cost. In this paper, a rejection cascade was built for LP localization with reduced Haar-like features. We first introduced line segment features as pre-input of Haarlike features for AdaBoost to eliminate more than 70% of the background in an image. Line segment features, including density, directionality and regularity, were extracted from line segments, which were detected by applying Hough Transform on an edge image. Later, AdaBoost classifiers with Haar-like features were further applied to identify the exact location of license plates. Our method dramatically reduced the demanded dimensions of Haar-like features, therefore saved much time in AdaBoost training stage. By comparing our method with methods of only using Haar-like features and only using line segment features, experimental results demonstrated that our proposed method achieved the best detection rate with significantly reduced dimensions of Haar-like features. © 2011 IEEE.
- Published
- 2011
21. Using context saliency for movie shot classification
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Xu, M, Wang, J, Hasan, MA, He, X, Xu, C, Lu, H, Jin, JS, Xu, M, Wang, J, Hasan, MA, He, X, Xu, C, Lu, H, and Jin, JS
- Abstract
Movie shot classification is vital but challenging task due to various movie genres, different movie shooting techniques and much more shot types than other video domain. Variety of shot types are used in movies in order to attract audiences attention and enhance their watching experience. In this paper, we introduce context saliency to measure visual attention distributed in keyframes for movie shot classification. Different from traditional saliency maps, context saliency map is generated by removing redundancy from contrast saliency and incorporating geometry constrains. Context saliency is later combined with color and texture features to generate feature vectors. Support Vector Machine (SVM) is used to classify keyframes into pre-defined shot classes. Different from the existing works of either performing in a certain movie genre or classifying movie shot into limited directing semantic classes, the proposed method has three unique features: 1) context saliency significantly improves movie shot classification; 2) our method works for all movie genres; 3) our method deals with the most common types of video shots in movies. The experimental results indicate that the proposed method is effective and efficient for movie shot classification. © 2011 IEEE.
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- 2011
22. A close-up detection method for movies
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Liu, H, Xu, M, Huang, Q, Jin, JS, Jiang, S, Xu, C, Liu, H, Xu, M, Huang, Q, Jin, JS, Jiang, S, and Xu, C
- Abstract
Close-up (CU) is a photographic technique which tightly frames a person or an object. In movies, it is applied to guide audience attention and to evoke audience emotion. In this paper, we detect face CU, object CU, and lean of movies, which are widely used to romance emotions. A lean consists of shots in a sequence, with a close-up shot as focus. A set of features are extracted by considering movie making techniques and human attention for CU detection. The features are average saliency, color entropy, color variance, face height, skin area, and texture scales. These features are tested through statistical hypothesis test to be significantly discriminating for CUs. Then, Support Vector Machine (SVM) is applied on these features to detect face CU and object CU. Based on the face CU and object CU detection result, lean is further detected by investigating the changing of the face/object size. Lean detection is of challenge due to the technique of montage. We solve this problem through color similarity estimation and SIFT point matching. Experimental results on four full length movies verify the effectiveness of the proposed method. © 2010 IEEE.
- Published
- 2010
23. Using scripts for affective content retrieval
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Xu, M, He, X, Jin, JS, Peng, Y, Xu, C, Guo, W, Xu, M, He, X, Jin, JS, Peng, Y, Xu, C, and Guo, W
- Abstract
Movie affective content analysis attracts increasing research efforts since affective content not only affect users attentions but also locate movie highlights. However, affective content retrieval is still a challenging task due to the limitation of affective features in movies. Scripts provide direct access to the movie content and represent affective aspects of the movie. In this paper, we utilize scripts as an important clue to retrieve video affective content. The proposed approach includes two main steps. Firstly, affective script partitions are extracted by detecting emotional words. Secondly, affective partitions are validated by using visual and auditory features. The results are encouraging and compared with the manually labelled ground truth. © 2010 Springer-Verlag.
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- 2010
24. Computer aided abnormality detection for microscopy images of cervical tissue
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Cui, Y, Jin, JS, Park, M, Luo, S, Xu, M, Peng, Y, Felix Wong, WS, Santos, LD, Cui, Y, Jin, JS, Park, M, Luo, S, Xu, M, Peng, Y, Felix Wong, WS, and Santos, LD
- Abstract
Cervical cancer is the second most common malignancy among women worldwide, if it is detected in early stage, cure rate is relatively high. Computer aided abnormality detection for cervical smear is developed to assist medical experts to handle the microscopy images, examine cell abnormalities and diagnose dyskaryosis. The microscopy images of cells in cervix uteri are stained by the tumor marker Ki-67, so that the abnormal nuclei present brown while normal ones are bluish. Segmentation is the most important and difficult task to calculate the ratio of abnormal nuclei to all nuclei. In order to achieve accurate segmentation of nuclei, we propose a multi-level segmentation approach for abnormality identification in microscopy images. First level segmentation aims to partition abnormal (stained) nuclei regions and all nuclei regions. Because of under-segmentation after first level segmentation, second level segmentation is applied to further partition the clustered nuclei. In order to classify touching regions of clustered nuclei and separate regions of single nucleus, relevant meaningful features are extracted from regions of interest. Consequently all the nuclei regions are separated and in conjunction with the abnormal nuclei regions in the first level segmentation, the abnormality i.e. ratio of abnormal nuclei to all nuclei is obtained. Experimental results indicate that our method achieved an accuracy of 93.55% and 95.8% in term of abnormal nuclei and all nuclei respectively for identification of abnormalities. Our proposed method produces a satisfactory segmentation. © 2010 IEEE.
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- 2010
25. Learning priors for super-resolution in video sequence
- Author
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Peng, Y, Jin, JS, Luo, S, Xu, M, Peng, Y, Jin, JS, Luo, S, and Xu, M
- Abstract
Video becomes a crucial information resource in last decades, because of the rapid development of camera as well as the internet explosion. High-quality video sequences are always desired in lots of fields. Since the bottleneck of data storage and interferences of shooting condition, we cannot always obtain high-resolution video. This botheration can be circumvented by super-resolution. Currently, almost super-resolution techniques are in the framework of Maximum a Posterior (MAP). Appropriate parameters of prior distribution are crucial for recovering accurate super-resolution image. We utilise a novel Weighted Cross Validation (WCG) method to learn theses prior parameters. Comparison experiments are provided to illustrate the effectiveness of our approach. Copyright 2010 ACM.
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- 2010
26. Automatic cell segmentation in microscopic color images using ellipse fitting and watershed
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Park, M, Jin, JS, Peng, Y, Summons, P, Yu, D, Cui, Y, Luo, S, Wang, F, Santos, L, Xu, M, Park, M, Jin, JS, Peng, Y, Summons, P, Yu, D, Cui, Y, Luo, S, Wang, F, Santos, L, and Xu, M
- Abstract
This paper presents an efficient and innovative method for the automated counting of cells in a microscopic image. The performance of watershed-based algorithms for the segmentation of clustered cells has been well demonstrated. The strength of our algorithm lies in the fact that it incorporates knowledge of color in the image. Our method uses the watershed transform with iterative shape alignment and is shown to be more accurate in retaining cell shape. We report a sensitivity of 97% and specificity of 96% when all color bands are used. Our methods could be of value to computer-based systems designed to objectively interpret microscopic images, since they provide a means for accurate cell segmentation. © 2010 IEEE.
- Published
- 2010
27. Clustering nuclei using machine learning techniques
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Peng, Y, Park, M, Xu, M, Luo, S, Jin, JS, Cui, Y, Felix Wong, WS, Santos, LD, Peng, Y, Park, M, Xu, M, Luo, S, Jin, JS, Cui, Y, Felix Wong, WS, and Santos, LD
- Abstract
Cervical cancer is the second most common cancer among women. Meanwhile, cervical cancer could be largely preventable and curable with regular Pap tests. Nuclei changes in the cervix could be found by this test. Accurate nuclei detection is extremely critical as it is the previous step of analysing nuclei changes and diagnosis afterwards. Recently, computer-aided nuclei segmentation has increased dramatically. Although such algorithms could be utilised in the situation for sparse nuclei since they are intuitively detected, the segmentation for the complicated nuclei clusters is still challenging task. This paper presents a new methodology for the detection of cervical nuclei clusters. We first detect all the nuclei from the cervical microscopic image by an ellipse fitting algorithm. Second, we chose some high-relevant features from all the features we obtained in last step via F-score, which is based on to what extent one feature attributes to results. All the ellipses are then classified into single ones and cluster ones by C4.5 decision tree with selected features. We evaluated the performance of this method by the classification accuracy, sensitivity, and cluster predictive value. With the 9 selected features from the original 13 features, we came by the promising classification accuracy (97.8%). © 2010 IEEE.
- Published
- 2010
28. Adaptive Local Hyperplanes for MTV affective analysis
- Author
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Xu, M, Chen, L, He, X, Xu, C, Jin, JS, Xu, M, Chen, L, He, X, Xu, C, and Jin, JS
- Abstract
Affective analysis attracts increasing attention in multimedia domain since affective factors directly reflect audiences' attention, evaluation and memory. Existing study focuses on mapping low-level affective features to high-level emotions by applying machine learning methods. Therefore, choosing effective features and developing efficient machine learning algorithms become vital for affective analysis. In this paper, we investigate the effectiveness of a novel classification approach, called Adaptive Local Hyperplanes (ALH), in affective analysis. The reason ALH is appealing in affective analysis is two-fold. Firstly, affective features are not equally important for emotion categories; ALH inherently assigns feature weights based on discriminative ability of each feature. Secondly, ALH achieves competitive performance with state-of-the-art classifiers (e.g., SVM) while it is designed for multi-class classification. Consequently, it is worthwhile to explore the usage of ALH in affective analysis. MTV data are used in this study. As the first effort of applying ALH to affective analysis, the results presented in this paper provide a foundation for future research in affective analysis. Copyright 2010 ACM.
- Published
- 2010
29. Framework for script based virtual directing and multimedia authoring in live video streaming
- Author
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Xu, RYD, Jin, JS, Allen, JG, Xu, RYD, Jin, JS, and Allen, JG
- Abstract
We propose a novel framework that facilitates automatic editing and authoring of multimedia using static and moving cameras in a dynamic scene. The framework incorporates several video techniques such as object tracking using mean shift and object recognition using Scaled Invariant Feature Transform (SIFT). These techniques are linked together by a comprehensive yet simple-to-program script authoring mechanism based on video event detection. These combined features empower the system to play a virtual director role in live video stream editing and multimedia integration. The system requires minimum human intervention and can leverage production efficiency for both novice and professional users. The experimental results from our prototype system demonstrate that this framework is achievable using inexpensive hardware and standard video cameras. Our system provides comprehensive pre-production authoring capabilities that lend towards integration of video and heterogonous multimedia elements in realtime. We have found this framework to be useful in many applications such as live video streaming, distance education, live entertainment, sports coverage and personal video broadcasting. © 2005 IEEE.
- Published
- 2005
30. Mean shift object tracking for a SIMD computer
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Allen, JG, Xu, RYD, Jin, JS, Allen, JG, Xu, RYD, and Jin, JS
- Abstract
We use SIMD instructions to implement a popular video object-tracking algorithm in an attempt to achieve the best possible performance on the available hardware. We start with an implementation of the well-known mean shift algorithm with adaptive scale and background-weighted histogram enhancements. Due to its histogramic nature, mean shift is not normally considered worth optimizing with vector instructions. However, when we looked for basic opportunities to exploit SIMD style processing, we in fact discovered several opportunities to exploit SIMD instructions. We compare the tracking efficiency of our implementation with a standard implementation in order to demonstrate the efficiency of the modified technique. In this paper we present the optimizations we have applied as well as the performance results obtained. © 2005 IEEE.
- Published
- 2005
31. Scheduling latency insensitive computer vision tasks
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Xu, RYD, Jin, JS, Xu, RYD, and Jin, JS
- Abstract
In recent times, there are increasing numbers of computer vision and pattern recognition (CVPR) technologies being applied to real time video processing using single processor PCs. However, these multiple computational expensive tasks are generating bottlenecks in real-time processing. We propose a scheme to achieve both high throughput and accommodation to user-specified scheduling rules. The scheduler is then distributing 'slices' of the latency insensitive tasks such as video object recognition and facial localization among the latency sensitive ones. We show our proposed work in detail, and illustrating its application in a real-time e-learning streaming system. We also provide discussions into the scheduling implementations, where a novel concept using interleaved SIMD execution is discussed. The experiments have indicated successful scheduling results on a high end consumer grade PC. © Springer-Verlag Berlin Heidelberg 2005.
- Published
- 2005
32. Dependence testing and vectorization of multimedia agents
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Allen, JG, Xu, RYD, Jin, JS, Allen, JG, Xu, RYD, and Jin, JS
- Abstract
We present a dependence testing algorithm that considers the short width of modern SIMD registers in a typical microprocessor. The test works by solving the dependence system with the generalized GCD algorithm and then simplifying the solution equations for a particular set of dependence distances. We start by simplifying each solution lattice to generate points that satisfy some small constant dependence distance that corresponds to the width of the register being used. We use the Power Test to efficiently perform Fourier-Motzkin Variable Elimination on the simplified systems in order to determine if dependences exist. The improvements described in this paper also extend our SIMD dependence test to loops with symbolic and triangular lower and upper bounds as well as array indices that contain unknown symbolic additive constants. The resulting analysis is used to guide the vectorization pass of a dynamic multimedia compiler used to compile software agents that process audio, video and image data. We fully detail the proposed dependence test in this paper, including the related work.
- Published
- 2005
33. Stream-based interactive video language authoring using correlated audiovisual watermarking
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Xu, RYD, Jin, JS, Allen, JG, Xu, RYD, Jin, JS, and Allen, JG
- Abstract
We propose a novel framework that employs correlated digital video and audio watermarking where the watermarking sequence contains video interaction information and media features as a basis towards constructing a secure, self-contained, format independent stream-based scheme for interactive media authoring. We have detailed our methods and illustrate the advantages of this scheme over the traditional meta-tag approach for interactive video authoring. We also identify many of the potential applications that can benefit from this approach. We outline the prototype application and demonstrate the successful implementation within our existing real-time multimedia authoring system, where the new post production video delivering module is based on this framework. © 2005 IEEE.
- Published
- 2005
34. Robust mean-shift tracking with extended fast colour thresholding
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Xu, RYD, Allen, JG, Jin, JS, Xu, RYD, Allen, JG, and Jin, JS
- Abstract
We propose a novel adaptive approach for object tracking using fast colour thresholding and region merging. It proves to be an effective measure against large variations between consecutive frames during a mean-shift process. The approach retains mean-shift's property of efficiency and improves mean-shift's drawback of robustness. It can track non-rigid objects with significant occlusion.
- Published
- 2004
35. Structure of alkali tellurite glasses from neutron diffraction and molecular orbital calculations
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Niida, H, Uchino, T, Jin, JS, Kim, SH, Fukunaga, T, Yoko, T, Niida, H, Uchino, T, Jin, JS, Kim, SH, Fukunaga, T, and Yoko, T
- Published
- 2001
36. Structure of alkali tellurite glasses from neutron diffraction and molecular orbital calculations
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60142072, Niida, H, Uchino, T, Jin, JS, Kim, SH, Fukunaga, T, Yoko, T, 60142072, Niida, H, Uchino, T, Jin, JS, Kim, SH, Fukunaga, T, and Yoko, T
- Published
- 2001
37. Structural study of PbO-B2O3 classes by X-ray diffraction and B-11 MAS NMR techniques
- Author
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Takaishi, T, Jin, JS, Uchino, T, Yoko, T, Takaishi, T, Jin, JS, Uchino, T, and Yoko, T
- Published
- 2000
38. Nanocrystallization behaviour of a ternary amorphous alloy during isothermalannealing: a Monte Carlo simulation.
- Author
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Jin JS Shi-Feng, Wang WW Wei-Min, Zhou ZJ Jian-Kun, Guo GH Hong-Xuan, J. JFW Webb, and Bian BX Xiu-Fang
- Published
- 2005
39. Detectivity of Fe Kα Lines in Gamma-RayBursts by Cerenkov Line Mechanism.
- Author
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Su SJ Jie and Jin JS Sheng-Zhen
- Published
- 2005
40. Detectivity of Fe Kα Lines in Gamma-Ray Bursts by Cerenkov Line Mechanism
- Author
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Jie, Su SJ and Sheng-Zhen, Jin JS
- Abstract
The Fe Kα lines in gamma-ray bursts (GRBs) produced with the Cerenkov line mechanism are studied. We theoretically predict the Fe Kα line luminosities in both the early (before 1 hour) and late (~1 day) afterglows. Assuming about 200 GRBs could be detected by Swift per year, we sampled the redshift of these GRBs using the Monte Carlo method according to the GRB formation rate derived from the statistical correlation between the spectral peak energy and the peak luminosity of GRBs. Then we obtain the Fe Kα line flux distributions of the simulated GRB sample in the early and late phases. The simulated results show that the iron line flux is relatively low, so the line detection would be still a rare event at present. In addition, our results suggest that the iron lines from GRBs could be detected in the high redshift: z~3 for the early phase and z~6 for the late phase. Therefore, it is possible that the identification of Fe Kα lines in GRBs provides a tool to directly measure redshift and to study the high-redshift GRBs in the Swift era.
- Published
- 2005
41. ProtonationDriven Polarization Retention Failure in Nano-Columnar Lead-Free Ferroelectric Thin Films.
- Author
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Sheeraz M, Ahn CW, Duong NX, Hwang SY, Jang JS, Kim EY, Kim YK, Lee J, Jin JS, Bae JS, Lee MH, Han HS, Kim GY, Cho S, Song TK, Yang SM, Bu SD, Baek SH, Choi SY, Kim IW, and Kim TH
- Abstract
Understanding microscopic mechanisms of polarization retention characteristics in ferroelectric thin films is of great significance for exploring unusual physical phenomena inaccessible in the bulk counterparts and for realizing thin-film-based functional electronic devices. Perovskite (K,Na)NbO
3 is an excellent class of lead-free ferroelectric oxides attracting tremendous interest thanks to its potential applications to nonvolatile memory and eco-friendly energy harvester/storage. Nonetheless, in-depth investigation of ferroelectric properties of (K,Na)NbO3 films and the following developments of nano-devices are limited due to challenging thin-film fabrication associated with nonstoichiometry by volatile K and Na atoms. Herein, ferroelectric (K,Na)NbO3 films of which the atomic-level geometrical structures strongly depend on thickness-dependent strain relaxation are epitaxially grown. Nanopillar crystal structures are identified in fully relaxed (K,Na)NbO3 films to the bulk states representing a continuous reduction of switchable polarization under air environments, that is, polarization retention failures. Protonation by water dissociation is responsible for the humidity-induced retention loss in nano-columnar (K,Na)NbO3 films. The protonation-driven polarization retention failure originates from domain wall pinning by the accumulation of mobile hydrogen ions at charged domain walls for effective screening of polarization-bound charges. Conceptually, the results will be utilized for rational design to advanced energy materials such as photo-catalysts enabling ferroelectric tuning of water splitting., (© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)- Published
- 2024
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42. Lactobacillus plantarum LPYC225 mixture partially modulates the vaginal bacterial community of Gardnerella vaginalis -infected bacterial vaginosis in mice.
- Author
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Kim HJ, Kim DG, Kang SH, Choi H, Go SW, Kim DK, Myung JH, Park JH, Soh JR, Kim SK, Hong SW, Kang M, Park ES, Park I, Kang J, Kwon HJ, and Jin JS
- Abstract
Bacterial vaginosis (BV) is defined as dysbiosis of the vaginal microbiome associated with the depletion of Lactobacilli and excessive growth of commensal or pathogenic bacteria. This study investigated the effects of lactic acid bacteria (LAB) mixture (LM; InoRexyne™) on the vaginal bacterial community of Gardnerella vaginalis ( G. vaginalis )-infected BV mice. Single LAB and LM exhibited antibacterial and anti-inflammatory effects by inhibiting G. vaginalis growth and pro-inflammatory markers in RAW 264.7 cells. Administering LM did not significantly alter the vaginal architecture or fecal short-chain fatty acids but did significantly inhibit the vaginal interleukin-6 levels in the high LM group compared to the GV group. LM administration decreased the relative abundances of Enterobacter, Escherichia coli , and Bacteroides vulgatus in vaginal flushing fluids compared to the GV group. LM partially alleviated BV by inhibiting G. vaginalis growth and modulating the vaginal bacterial community, providing new insights into its modulatory effects on the vaginal microbiome in BV., Supplementary Information: The online version contains supplementary material available at 10.1007/s10068-024-01641-w., Competing Interests: Conflict of interestOn behalf of all authors, the corresponding author states that there is no conflict of interest., (© The Korean Society of Food Science and Technology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
- Published
- 2024
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43. Effect of Jianpi Shengxue Tablet on Iron Metabolism and Nutritional Status in Patients with Renal Anemia: A Prospective, Randomized, Open, Parallel Controlled and Multicenter Clinical Study.
- Author
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Yang J, Liang MY, Li Y, Zhang H, Li W, Lv J, Dong LP, Jin JS, Yang C, Zhang QH, Long L, Zou R, Gao Y, Song D, Pan CQ, Yao Y, Zhan XL, and Wang XH
- Subjects
- Humans, Male, Female, Middle Aged, Prospective Studies, Tablets, Adult, Anemia drug therapy, Anemia metabolism, Anemia blood, Aged, Treatment Outcome, Hematocrit, Ferritins blood, Erythrocyte Count, Iron metabolism, Iron blood, Drugs, Chinese Herbal administration & dosage, Drugs, Chinese Herbal pharmacology, Drugs, Chinese Herbal therapeutic use, Nutritional Status drug effects
- Abstract
Objective: This study aimed to analyze the clinical efficacy of the Jianpi Shengxue tablet for treating renal anemia., Methods: A total of 200 patients with renal anemia from December 2020 to December 2022 were enrolled and randomly divided into two groups. Patients in the control group were treated with polysaccharide-iron complex, and those in the experimental group were administered Jianpi Shengxue tablet. After 8 weeks of continuous treatment, the therapeutic outcomes regarding anemia were compared between the two groups., Results: After treatment, the red blood cell (RBC) count, hematocrit (HCT), reticulocyte percentage (RET), ferritin (SF), serum iron (SI), transferrin saturation (TSAT), and serum albumin (ALB) all increased (P<0.01), and the clinical symptom score and total iron binding capacity decreased (P<0.01) in the experimental group. Moreover, the improvements in RBC, HCT, RET, SF, SI, TAST, ALB, and clinical symptoms (fatigue, anorexia, dull skin complexion, numbness of hands and feet) in the experimental group were significantly greater than those in the control group (P<0.05). The total effective rate for treating renal anemia was significantly higher in the experimental group than in the control group (P<0.01)., Conclusion: The Jianpi Shengxue tablet demonstrates efficacy in treating renal anemia, leading to significant improvements in the laboratory examination results and clinical symptoms of patients with renal anemia., (© 2024. Huazhong University of Science and Technology.)
- Published
- 2024
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44. Gonadal efficacy of Thymus quinquecostatus Celakovski: Regulation of testosterone levels in aging mouse models.
- Author
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Kim HY, Kwon HS, Lim JO, Jang HJ, Muthamil S, Shin UC, Lyu JH, Park YJ, Nam HH, Lee NY, Oh HJ, Yun SI, Jin JS, and Park JH
- Subjects
- Animals, Male, Mice, Leydig Cells drug effects, Leydig Cells metabolism, Cell Survival drug effects, Cell Line, Hypogonadism drug therapy, Disease Models, Animal, Testosterone blood, Aging drug effects, Aging metabolism, Plant Extracts pharmacology, Testis drug effects, Testis metabolism, Thymus Plant chemistry
- Abstract
Late-onset hypogonadism (LOH) is an age-related disease in men characterized by decreased testosterone levels with symptoms such as decreased libido, erectile dysfunction, and depression. Thymus quinquecostatus Celakovski (TQC) is a plant used as a volatile oil in traditional medicine, and its bioactive compounds have anti-inflammatory potential. Based on this knowledge, the present study aimed to investigate the effects of TQC extract (TE) on LOH in TM3 Leydig cells and in an in vivo aging mouse model. The aqueous extract of T. quinquecostatus Celakovski (12.5, 25, and 50 µg/mL concentrations) was used to measure parameters such as cell viability, testosterone level, body weight, and gene expression, via in vivo studies. Interestingly, TE increased testosterone levels in TM3 cells in a dose-dependent manner without affecting cell viability. Furthermore, TE significantly increased the expression of genes involved in the cytochrome P450 family (Cyp11a1, Cyp17a1, Cyp19a1, and Srd5a2), which regulate testosterone biosynthesis. In aging mouse models, TE increased testosterone levels without affecting body weight and testicular tissue weight tissue of an aging animal group. In addition, the high-dose TE-treated group (50 mg/kg) showed significantly increased expression of the cytochrome p450 enzymes, similar to the in vitro results. Furthermore, HPLC-MS analysis confirmed the presence of caffeic acid and rosmarinic acid as bioactive compounds in TE. Thus, the results obtained in the present study confirmed that TQC and its bioactive compounds can be used for LOH treatment to enhance testosterone production., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Jun Hong Park declare that this study was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest., (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Published
- 2024
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45. Phenylacetic acid, an anti-vaginitis metabolite produced by the vaginal symbiotic bacterium Chryseobacterium gleum.
- Author
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Kwon KM, Kim EH, Sim KH, Lee YJ, Kang EJ, Han KH, Jin JS, Kim DK, Ahn JH, and Hwang IH
- Subjects
- Female, Animals, Mice, Humans, Candida albicans metabolism, Candida albicans drug effects, Symbiosis, Hydrogen-Ion Concentration, Gardnerella vaginalis metabolism, Gardnerella vaginalis drug effects, Disease Models, Animal, Vaginitis microbiology, Vaginitis metabolism, Vaginitis drug therapy, Phenylacetates metabolism, Phenylacetates pharmacology, Vagina microbiology, Chryseobacterium metabolism
- Abstract
The human microbiome contains genetic information that regulates metabolic processes in response to host health and disease. While acidic vaginal pH is maintained in normal conditions, the pH level increases in infectious vaginitis. We propose that this change in the vaginal environment triggers the biosynthesis of anti-vaginitis metabolites. Gene expression levels of Chryseobacterium gleum, a vaginal symbiotic bacterium, were found to be affected by pH changes. The distinctive difference in the metabolic profiles between two C. gleum cultures incubated under acidic and neutral pH conditions was suggested to be an anti-vaginitis molecule, which was identified as phenylacetic acid (PAA) by spectroscopic data analysis. The antimicrobial activity of PAA was evaluated in vitro, showing greater toxicity toward Gardnerella vaginalis and Candida albicans, two major vaginal pathogens, relative to commensal Lactobacillus spp. The activation of myeloperoxidase, prostaglandin E
2 , and nuclear factor-κB, and the expression of cyclooxygenase-2 were reduced by an intravaginal administration of PAA in the vaginitis mouse model. In addition, PAA displayed the downregulation of mast cell activation. Therefore, PAA was suggested to be a messenger molecule that mediates interactions between the human microbiome and vaginal health., (© 2024. The Author(s).)- Published
- 2024
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46. Adlercreutzia faecimuris sp. nov., producing propionate and acetate isolated from mouse feces.
- Author
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Suh MK, Jin JS, Do HE, Kim JS, Eom MK, Kim HS, and Lee JS
- Subjects
- Animals, Mice, DNA, Bacterial genetics, Fatty Acids metabolism, Fatty Acids analysis, Bacterial Typing Techniques, Sequence Analysis, DNA, Genome, Bacterial, Phylogeny, Feces microbiology, RNA, Ribosomal, 16S genetics, Mice, Inbred BALB C, Base Composition, Acetates metabolism, Propionates metabolism
- Abstract
A novel strictly anaerobic bacterium, strain JBNU-10
T , was isolated from BALB/c mouse feces. Cells of the strain JBNU-10T were Gram-stain positive, non-motile and rod-shaped. Optimum growth occurred at 37℃, with 1% (w/v) NaCl and at pH 7. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain JBNU-10T belonged to the genus Adlercreutzia and were closely related to Adlercreutzia muris WCA-131-CoC-2T (95.90%). The genome sequencing of strain JBNU-10T revealed a genome size of 2,790,983 bp, a DNA G + C content of 69.4 mol%. It contains a total of 2,266 CDSs, 5 rRNA genes and 49 tRNA genes. According to the data obtained strain JBNU-10T shared ANI value below 77.6- 67.7%, dDDH value below 23.8% with the closely type species. Strain JBNU-10T possessed iso-C16:0 DMA, C18:1 CIS 9 FAME, and C18:0 DMA as the major fatty acids and had DMMK-6. The major end products of fermentation is propionate and acetate. Based on phylogenetic, physiological and chemotaxonomic characteristics, strain JBNU-10T represent a novel species of the genus Adlercreutzia. The type strain is JBNU-10T (= KCTC 25028T = CCUG 75610T )., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)- Published
- 2024
- Full Text
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47. Effectively α-Terpineol Suppresses Glioblastoma Aggressive Behavior and Downregulates KDELC2 Expression.
- Author
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Jin JS, Chou JM, Tsai WC, Chen YC, Chen Y, Ong JR, and Tsai YL
- Subjects
- Mice, Animals, Humans, Phosphatidylinositol 3-Kinases, Endothelial Cells metabolism, TOR Serine-Threonine Kinases, Phosphatidylinositol 3-Kinase, Cell Line, Tumor, Drug Resistance, Neoplasm, Mammals, Glioblastoma drug therapy, Glioblastoma metabolism, Brain Neoplasms drug therapy, Cyclohexane Monoterpenes
- Abstract
Background: Glioblastoma (GBM) is notorious for the aggressive behaviors and easily results in chemo-resistance. Studies have shown that the use of herbal medicines as treatments for GBM as limited by the blood-brain barrier (BBB) and glioma stem cells., Purpose: The aim of this study was to investigate the relationship between GBM suppression and α-terpineol, the monoterpenoid alcohol derived from Eucalyptus glubulus and Pinus merkusii., Study Design: Using serial in-vitro and in-vivo studies to confirm the mechanism of α-terpineol on down-regulating GBM development., Methods: The 3-[4,5-dimethylthiazol-2-yl)]-2,5-diphenyltetrazolium bromide (MTT) assay was performed to evaluate IC50 of α-terpineol to inhibit GBM cell survival. In order to evaluate the impact of GBM aggressive behaviors by α-terpineol, the analysis of cell migration, invasion and colony formation were implemented. In addition, the ability of tumor spheres and WB of CD44 and OCT3/4 were evaluated under the impression of α-terpineol decreased GBM stemness. The regulation of neoangiogenesis by α-terpineol via the WB of angiogenic factors and human umbilical vein endothelial cells (HUVEC) tube assay. To survey the decided factors of α-terpineol downregulating GBM chemoresistance depended on the impact of O6-methylguanine-DNA methyltransferase (MGMT) expression and autophagy-related factors activation. Additionally, WB and quantitative real-time polymerase chain reaction (qRT/PCR) of KDEL (Lys-Asp-Glu-Leu) containing 2 (KDELC2), endoplasmic reticulum (ER) stress, phosphoinositide 3-kinase (PI3k), mammalian target of rapamycin (mTOR) and mitogen-activated protein kinase (MAPK) cascade signaling factors were examined to explore the mechanism of α-terpineol inhibiting GBM viability. Finally, the orthotopic GBM mouse model was applied to prove the efficacy and toxicity of α-terpineol on regulating GBM survival., Results: α-terpineol significantly suppressed GBM growth, migration, invasion, angiogenesis and temozolomide (TMZ) resistance. Furthermore, α-terpineol specifically targeted KDELC2 to downregulate Notch and PI3k/mTOR/MAPK signaling pathway. Finally, we also demonstrated that α-terpineol could penetrate the BBB to inhibit GBM proliferation, which resulted in reduced cytotoxicity to vital organs., Conclusion: Compared to published literatures, we firstly proved α-terpineol possessed the capability to inhibit GBM through various mechanisms and potentially decreased the occurrence of chemoresistance, making it a promising alternative therapeutic option for GBM in the future., Competing Interests: Declaration of competing interest This manuscript has not been published or presented elsewhere in part or in entirety and is not under consideration by another journal. The study design was approved by the appropriate ethics review board. We have read and understood your journal's policies, and we believe that neither the manuscript nor the study violates any of these. All authors declare that there is no conflict of interest in this study., (Copyright © 2024 Elsevier GmbH. All rights reserved.)
- Published
- 2024
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48. Targeting pyruvate dehydrogenase kinase 1 overcomes EGFR C797S mutation-driven osimertinib resistance in non-small cell lung cancer.
- Author
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Park W, Wei S, Xie CL, Han JH, Kim BS, Kim B, Jin JS, Yang ES, Cho MK, Ryu D, Yang HX, Bae SJ, and Ha KT
- Subjects
- Humans, Animals, Cell Line, Tumor, Mice, Xenograft Model Antitumor Assays, Protein Kinase Inhibitors pharmacology, Protein Kinase Inhibitors therapeutic use, Female, Male, Indoles, Pyrimidines, Acrylamides pharmacology, Acrylamides therapeutic use, ErbB Receptors genetics, ErbB Receptors metabolism, Drug Resistance, Neoplasm genetics, Carcinoma, Non-Small-Cell Lung drug therapy, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung pathology, Carcinoma, Non-Small-Cell Lung metabolism, Aniline Compounds pharmacology, Aniline Compounds therapeutic use, Lung Neoplasms drug therapy, Lung Neoplasms genetics, Lung Neoplasms pathology, Lung Neoplasms metabolism, Pyruvate Dehydrogenase Acetyl-Transferring Kinase metabolism, Pyruvate Dehydrogenase Acetyl-Transferring Kinase genetics, Mutation
- Abstract
Osimertinib, a selective third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), effectively targets the EGFR T790M mutant in non-small cell lung cancer (NSCLC). However, the newly identified EGFR C797S mutation confers resistance to osimertinib. In this study, we explored the role of pyruvate dehydrogenase kinase 1 (PDK1) in osimertinib resistance. Patients exhibiting osimertinib resistance initially displayed elevated PDK1 expression. Osimertinib-resistant cell lines with the EGFR C797S mutation were established using A549, NCI-H292, PC-9, and NCI-H1975 NSCLC cells for both in vitro and in vivo investigations. These EGFR C797S mutant cells exhibited heightened phosphorylation of EGFR, leading to the activation of downstream oncogenic pathways. The EGFR C797S mutation appeared to increase PDK1-driven glycolysis through the EGFR/AKT/HIF-1α axis. Combining osimertinib with the PDK1 inhibitor leelamine helped successfully overcome osimertinib resistance in allograft models. CRISPR-mediated PDK1 knockout effectively inhibited tumor formation in xenograft models. Our study established a clear link between the EGFR C797S mutation and elevated PDK1 expression, opening new avenues for the discovery of targeted therapies and improving our understanding of the roles of EGFR mutations in cancer progression., (© 2024. The Author(s).)
- Published
- 2024
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49. Calcium Chloride as a Novel Stabilizer for Foot-and-Mouth Disease Virus and Its Application in the Vaccine Formulation.
- Author
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Jin JS, Lee G, Kim JY, Lee S, Park JH, Park SY, and Ko YJ
- Abstract
The thermal stability of the in-house-developed foot-and-mouth disease (FMD) type O and A viruses was evaluated, and the O Jincheon virus was found to exhibit the lowest thermal stability. To overcome this instability, we proposed a novel stabilizer, calcium chloride. The thermal stability of FMDVs increased up to a CaCl
2 concentration of 10 mM, and it had a decreasing trend at >30 mM. The O Jincheon virus showed a significant decrease in the amount of antigen over time at 4 °C. In contrast, the samples treated with CaCl2 showed stable preservation of the virus without significant antigen loss. After the CaCl2 -formulated vaccine was administered twice to pigs, the virus neutralization titer reached approximately 1:1000, suggesting that the vaccine could protect pigs against the FMDV challenge. In summary, the O Jincheon virus is difficult to utilize as a vaccine given its low stability during storage after antigen production. However, following its treatment with CaCl2 , it can be easily utilized as a vaccine. This study evaluated CaCl2 as a novel stabilizer in FMD vaccines and may contribute to the development of stable vaccine formulations, especially for inherently unstable FMDV strains.- Published
- 2024
- Full Text
- View/download PDF
50. Determination of Optimal Antigen Yield and Virus Inactivation Conditions for the Production of the Candidate Foot-and-Mouth Disease Recombinant Vaccine Strain Asia1 Shamir-R in a Bioreactor.
- Author
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Kim JY, Park SY, Lee G, Park SH, Jin JS, Kim D, Park JH, Jeong SY, and Ko YJ
- Subjects
- Animals, Swine, Virus Inactivation, Capsid Proteins, Vaccines, Synthetic, Bioreactors, Foot-and-Mouth Disease, Foot-and-Mouth Disease Virus, Viral Vaccines
- Abstract
Since the foot-and-mouth disease (FMD) outbreak in South Korea in 2010-2011, vaccination policies utilizing inactivated FMD vaccines composed of types O and A have been implemented nationwide. However, because type Asia1 occurred in North Korea in 2007 and intermittently in neighboring countries, the risk of type Asia1 introduction cannot be ruled out. This study evaluated the antigen yield and viral inactivation kinetics of the recombinant Asia1 Shamir vaccine strain (Asia1 Shamir-R). When Asia1 Shamir-R was proliferated in shaking flasks (1 L), a 2 L bioreactor (1 L), and a wave bioreactor (25 L), the antigen yields were 7.5 μg/mL, 5.2 μg/mL, and 3.8 μg/mL, respectively. The optimal FMDV inactivation conditions were 2 mM BEI at 26 °C and 1.0 mM BEI at 37 °C. There was no antigen loss due to BEI treatment, and only a decrease in antigen levels was observed during storage. The sera from pigs immunized with antigen derived from a bioreactor exhibited a neutralizing antibody titer of approximately 1/1000 against Asia1 Shamir and Asia1/MOG/05 viruses; therefore, Asia1 Shamir-R is expected to provide sufficient protection against both viruses. If an FMD vaccine production facility is established, this Asia1 Shamir-R can be employed for domestic antigen banks in South Korea.
- Published
- 2024
- Full Text
- View/download PDF
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